FastQCFastQC Report
Thu 26 May 2016
SRR936434_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936434_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2973496
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT166680.5605522926548413No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89780.3019341542749679No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT81480.2740208831624458No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79870.268606381175559No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT65100.2189342107741191No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC57020.19176080949831445No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT49800.16747962667513258No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47100.158399405951782No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC43770.14720046705964965No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC43610.14666237990567332No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT40180.13512713654230574No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG34620.1164286079416283No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA33290.11195575847420007No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC32120.10802099616074815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG125650.022.9565451
TACATGG127200.022.4403652
GAGTACT109050.021.99198512-13
ACATGGG127850.021.4343533
AGTACTT112800.020.46095712-13
GTACTTT118200.020.26947214-15
GTGTAGC18800.020.1680451
TATTACG1200.00476848519.7893412
AGAGTAC200050.018.6231510-11
CATGGGG60500.017.9766224
TATAGCG1851.5243239E-417.9699545
ACTTTTT138400.017.414316-17
CTAGGCG1650.001466036317.2701154
TACTTTT134000.017.2061314-15
GTAGCCC16300.016.6081963
CATGGGA67850.016.589244
TAGCCCT18100.016.530654
CTAGACG2406.160514E-515.8309414
AGTACAT99100.015.2883132
GTATCAA349400.014.9314231
Produced by FastQC (version 0.11.5)