FastQCFastQC Report
Thu 26 May 2016
SRR936433_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936433_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2957160
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT149950.5070743551245114No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT95240.3220657658023238No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75980.2569357085852642No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT72600.2455058231546484No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62360.21087800457195416No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC58500.19782494014527452No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT46530.15734691393093372No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT40200.1359412409203425No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC38410.12988813591418794No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36040.12187368962112298No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC35100.1186949640871647No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC32700.11057906910684576No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG29690.10040038415236241No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG130200.024.4542771
TACATGG132000.023.3659692
ACATGGG133050.022.1457213
GAGTACT87150.021.32028612-13
AGTACTT92750.020.23796312-13
CATGGGG61300.018.994744
GTACTTT97600.018.9398314-15
AGAGTAC176500.018.38828710-11
ATTCGCG1951.4551915E-1118.26980680-81
TAGCCCT17600.017.8221054
ACCGTCG3059.246469E-817.1380658
TACTTTT110100.016.70320914-15
GTATAGC10350.016.529251
ACTTTTT115500.016.51773316-17
CATGGGA70950.016.0093354
AGTACAT101050.015.94323352
GTGTAGC18650.015.7980621
GAGTACA120900.015.4867721
GTATCAA320850.015.0333221
GTACCTG21500.015.0300861
Produced by FastQC (version 0.11.5)