##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936433_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2957160 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.208905503929447 31.0 31.0 34.0 28.0 34.0 2 31.242062654709247 31.0 31.0 34.0 28.0 34.0 3 31.298128948044745 31.0 31.0 34.0 28.0 34.0 4 34.91584222700158 37.0 35.0 37.0 32.0 37.0 5 34.88683094590756 37.0 35.0 37.0 32.0 37.0 6 35.01703222010307 37.0 35.0 37.0 32.0 37.0 7 34.8672381609382 37.0 35.0 37.0 32.0 37.0 8 34.81284036034574 37.0 35.0 37.0 32.0 37.0 9 36.255334510138105 39.0 35.0 39.0 32.0 39.0 10-11 36.162667221252825 39.0 35.0 39.0 31.5 39.0 12-13 36.04831020303264 39.0 35.0 39.0 30.0 39.0 14-15 37.052840225080814 39.0 36.0 41.0 31.0 41.0 16-17 36.93054095821667 39.0 36.0 41.0 31.0 41.0 18-19 36.87588784509462 39.0 36.0 41.0 30.5 41.0 20-21 36.77604086353122 39.0 36.0 41.0 30.0 41.0 22-23 36.64234485114096 39.0 36.0 40.5 30.0 41.0 24-25 36.78529873256774 39.0 36.0 41.0 30.0 41.0 26-27 36.768920856497445 39.0 36.0 41.0 30.0 41.0 28-29 36.681086752154094 39.0 36.0 41.0 30.0 41.0 30-31 36.51228966305509 39.0 36.0 41.0 30.0 41.0 32-33 36.346119756793684 39.0 35.0 40.5 30.0 41.0 34-35 36.11727400614103 39.0 35.0 40.0 29.0 41.0 36-37 35.92234204439394 38.0 35.0 40.0 28.5 41.0 38-39 35.71998539138903 38.0 35.0 40.0 27.0 41.0 40-41 35.56052377957229 38.0 35.0 40.0 27.0 41.0 42-43 35.32998180686876 38.0 34.0 40.0 26.5 41.0 44-45 35.07289561606406 38.0 34.0 40.0 25.5 41.0 46-47 34.80813178860799 38.0 34.0 40.0 24.5 41.0 48-49 34.71486967225311 38.0 34.0 40.0 24.0 41.0 50-51 33.78975232993818 37.5 32.0 39.5 23.5 40.5 52-53 33.94883334009658 37.5 33.0 39.5 23.5 40.5 54-55 34.380466224350386 38.0 33.5 40.0 24.0 41.0 56-57 34.27915855077168 38.0 33.5 40.0 23.0 41.0 58-59 34.01780965520973 38.0 33.0 40.0 23.0 41.0 60-61 33.676371586251676 37.0 33.0 40.0 22.0 41.0 62-63 33.3554361279065 37.0 32.0 40.0 21.5 41.0 64-65 33.01391723816094 36.0 32.0 39.0 20.5 41.0 66-67 32.596334828010654 36.0 31.5 39.0 19.5 41.0 68-69 32.18427156460929 35.0 31.0 39.0 18.0 40.0 70-71 31.733533187247225 35.0 31.0 38.0 16.0 40.0 72-73 31.33635075545456 35.0 30.5 37.0 13.5 39.5 74-75 30.90439560253757 35.0 30.0 37.0 11.0 39.0 76-77 30.47090519282014 34.5 30.0 36.0 7.5 39.0 78-79 30.005013763205234 34.0 29.0 36.0 7.0 38.5 80-81 29.509890570682682 34.0 29.0 35.0 2.0 37.0 82-83 29.013668688877168 34.0 29.0 35.0 2.0 37.0 84-85 28.688033620094956 34.0 28.5 35.0 2.0 36.0 86-87 28.376552503077278 34.0 27.5 35.0 2.0 36.0 88-89 27.94976142650381 33.5 27.0 35.0 2.0 35.5 90-91 27.519123246628524 33.0 26.0 35.0 2.0 35.0 92-93 27.130711730173545 33.0 25.5 35.0 2.0 35.0 94-95 26.64329474901595 33.0 25.0 35.0 2.0 35.0 96-97 26.026166490822277 33.0 24.5 35.0 2.0 35.0 98-99 25.289188613399343 32.0 20.5 35.0 2.0 35.0 100-101 22.740108583911592 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5805235912806808 1101 2 0.5370169184853317 1101 3 0.5088459350197283 1101 4 0.5160178565457372 1101 5 0.5179871752243699 1101 6 0.38230091375640285 1101 7 0.36171214616083347 1101 8 0.4691276367187527 1101 9 0.7279551152466723 1101 10-11 0.6625133102286327 1101 12-13 0.7190403072330938 1101 14-15 0.9736384101508406 1101 16-17 0.9725954854891015 1101 18-19 0.904439774770907 1101 20-21 0.8780744242658542 1101 22-23 0.9223860989467099 1101 24-25 0.9647355660173531 1101 26-27 0.7307590017796812 1101 28-29 0.7216518996140096 1101 30-31 0.7269035347393782 1101 32-33 0.6388147229056429 1101 34-35 0.7642362066613657 1101 36-37 0.6283722685958111 1101 38-39 0.5880346463459816 1101 40-41 0.6678079314429581 1101 42-43 0.6159259532878352 1101 44-45 0.5236729269383957 1101 46-47 0.6073933026382434 1101 48-49 0.7938789663806531 1101 50-51 0.7113577744301836 1101 52-53 0.6924064096279565 1101 54-55 0.8829744881073296 1101 56-57 0.7356137211638512 1101 58-59 0.7049785564814002 1101 60-61 0.6321453721810144 1101 62-63 0.6706010840118282 1101 64-65 0.6382031884517474 1101 66-67 0.7496900751917579 1101 68-69 0.6963239123575917 1101 70-71 0.6250843142022013 1101 72-73 0.49814402186335727 1101 74-75 0.3454729209325471 1101 76-77 0.6079589371126524 1101 78-79 0.5939462213448614 1101 80-81 0.6623475953723705 1101 82-83 0.8574610330026964 1101 84-85 0.852678310327363 1101 86-87 0.6823945768807604 1101 88-89 0.7796490104199663 1101 90-91 0.7012878388823545 1101 92-93 0.6972377840751207 1101 94-95 0.7355541334045377 1101 96-97 0.6367349944504035 1101 98-99 0.5580940306553792 1101 100-101 1.1593140461612599 1102 1 0.359646839977966 1102 2 0.4540461002203955 1102 3 0.25877389387263605 1102 4 0.354290049787501 1102 5 0.4022362795546073 1102 6 0.3538347706857934 1102 7 0.36237492471408217 1102 8 0.28431985454788844 1102 9 0.4706851845487492 1102 10-11 0.34078658383658933 1102 12-13 0.3991302728200097 1102 14-15 0.4149669452927611 1102 16-17 0.4190901992251952 1102 18-19 0.4757799389102644 1102 20-21 0.5045916811076481 1102 22-23 0.3201193983027295 1102 24-25 0.23754042959301103 1102 26-27 0.1904317733185792 1102 28-29 0.23819631220303705 1102 30-31 0.29792194855697574 1102 32-33 0.19394342002217257 1102 34-35 0.04667967368901316 1102 36-37 0.13111179009801788 1102 38-39 0.19096763974550868 1102 40-41 0.19685015267585015 1102 42-43 0.23750811543794725 1102 44-45 -0.01068723622501011 1102 46-47 0.1474894973997607 1102 48-49 0.19366897773252845 1102 50-51 0.28703526745047725 1102 52-53 0.23965149591969492 1102 54-55 0.4649035462027129 1102 56-57 0.42702925791407864 1102 58-59 0.5122895732244572 1102 60-61 0.3087711375491722 1102 62-63 0.4297442351427989 1102 64-65 0.45438806802405196 1102 66-67 0.44019658821348884 1102 68-69 0.23729383821595462 1102 70-71 0.23503542060361227 1102 72-73 0.3296253705860863 1102 74-75 0.24257135454213596 1102 76-77 0.4172589923559116 1102 78-79 0.13812225436470982 1102 80-81 0.31793935390530237 1102 82-83 0.45779758313640784 1102 84-85 0.4454168538772656 1102 86-87 0.38170215854128386 1102 88-89 0.5409219187516996 1102 90-91 0.6730674389581033 1102 92-93 0.526450363794801 1102 94-95 0.49866466832034817 1102 96-97 0.2600420274937427 1102 98-99 0.19699558740683543 1102 100-101 0.43701293846461553 1103 1 0.2692726485959156 1103 2 0.3706465241737611 1103 3 0.3171745549292311 1103 4 0.44171072984890003 1103 5 0.4900769726818126 1103 6 0.3425398170100138 1103 7 0.2619760978953778 1103 8 0.22277380672521474 1103 9 0.4637374089333761 1103 10-11 0.3866652306121665 1103 12-13 0.48801857161576123 1103 14-15 0.41413648438053485 1103 16-17 0.600837647738615 1103 18-19 0.4457007503149768 1103 20-21 0.5719139793186443 1103 22-23 0.6413667200413045 1103 24-25 0.6729022461509473 1103 26-27 0.4922951066062282 1103 28-29 0.2574082694696429 1103 30-31 0.30326605089968695 1103 32-33 0.5603479996778802 1103 34-35 0.39036742838082006 1103 36-37 0.5460540047518165 1103 38-39 0.5038815139391772 1103 40-41 0.4126921832205923 1103 42-43 0.4683301361148082 1103 44-45 0.5112025541739129 1103 46-47 0.7107250199405541 1103 48-49 0.6966965838122405 1103 50-51 0.7453785942965965 1103 52-53 0.7696341757343959 1103 54-55 0.8251289941405133 1103 56-57 0.7846047117908697 1103 58-59 0.7049903400409718 1103 60-61 0.6256071799810528 1103 62-63 0.8302969335054726 1103 64-65 0.8902518444526137 1103 66-67 0.7567265436252839 1103 68-69 0.807604482277867 1103 70-71 1.0779483925258369 1103 72-73 1.0024921553475146 1103 74-75 0.7583385479525866 1103 76-77 0.7967904793449314 1103 78-79 1.051634825632732 1103 80-81 0.8137495022889816 1103 82-83 0.7089124308695354 1103 84-85 0.7279829995107292 1103 86-87 0.9698629296064674 1103 88-89 1.1070114457787241 1103 90-91 0.9380818353270861 1103 92-93 0.8845845978006146 1103 94-95 0.9724339814976197 1103 96-97 1.0255335427158663 1103 98-99 1.323017067177684 1103 100-101 1.7522004795807824 1104 1 0.11952050674818437 1104 2 0.13302507919937412 1104 3 0.17254628695537377 1104 4 0.27147965318400225 1104 5 0.29853947930608626 1104 6 0.16190145806282885 1104 7 0.22335659604058122 1104 8 0.23549516779216617 1104 9 0.2274005205744274 1104 10-11 0.2991483939225432 1104 12-13 0.11030869261912102 1104 14-15 0.1874635798583668 1104 16-17 0.17285948575310073 1104 18-19 0.3182103003061414 1104 20-21 0.39986454017361694 1104 22-23 0.20369090083975294 1104 24-25 0.14401241882362115 1104 26-27 -0.05975979756264849 1104 28-29 0.18981172071500652 1104 30-31 0.08674870634925469 1104 32-33 -0.14587743531593134 1104 34-35 -0.16789524605832185 1104 36-37 -0.012737231337212052 1104 38-39 0.07218851579396812 1104 40-41 0.1356251345542816 1104 42-43 0.03024913231689652 1104 44-45 0.16609126641556315 1104 46-47 0.26870726689338653 1104 48-49 0.13939746484032867 1104 50-51 0.020042412526592557 1104 52-53 0.23901982977078973 1104 54-55 0.21007285344099103 1104 56-57 0.10250818511713788 1104 58-59 0.1627917995887529 1104 60-61 0.26321868854844155 1104 62-63 0.2860194759008081 1104 64-65 0.11994155254305383 1104 66-67 0.2458660507794974 1104 68-69 0.26598804966879186 1104 70-71 0.39038905009516967 1104 72-73 0.418529792249128 1104 74-75 0.12090313459805557 1104 76-77 0.4304773831605111 1104 78-79 0.33517102727555326 1104 80-81 0.24992133588728294 1104 82-83 0.4397122564303899 1104 84-85 0.3641545570977307 1104 86-87 0.40993038676951343 1104 88-89 0.484615612445392 1104 90-91 0.5633128567897252 1104 92-93 0.639156173052335 1104 94-95 0.5643838357636533 1104 96-97 0.6333929646377854 1104 98-99 0.8581740072059425 1104 100-101 1.2397570618983949 1105 1 0.1864836470729756 1105 2 0.15294949390534285 1105 3 0.16875044013768203 1105 4 0.12918395943199812 1105 5 0.3127259351794365 1105 6 0.24183545759634484 1105 7 0.1361610874251653 1105 8 0.1606396690895764 1105 9 0.37626655894289485 1105 10-11 0.3578617492681815 1105 12-13 0.22903263500811022 1105 14-15 0.022692012174175602 1105 16-17 0.2041982274073817 1105 18-19 0.2538089146972311 1105 20-21 0.227899249640366 1105 22-23 0.1601878103706369 1105 24-25 0.16655617009916313 1105 26-27 0.20977648629402523 1105 28-29 0.19942205920883538 1105 30-31 0.037351038049884266 1105 32-33 0.08462728056499458 1105 34-35 -0.056011426559216204 1105 36-37 0.053961688456368506 1105 38-39 0.029802713729765173 1105 40-41 -0.26096304950356597 1105 42-43 0.20607969589017472 1105 44-45 -0.08901101426317126 1105 46-47 0.13048813149585214 1105 48-49 0.2929436688112901 1105 50-51 0.24100311837807453 1105 52-53 0.18543080385166633 1105 54-55 0.20767081548069655 1105 56-57 0.45211167209927083 1105 58-59 0.43729360722163335 1105 60-61 0.23783174213601654 1105 62-63 0.31844507504363406 1105 64-65 0.5089712071977104 1105 66-67 0.5760470509544291 1105 68-69 0.5931496469456832 1105 70-71 0.6691180217853052 1105 72-73 0.6447903064040617 1105 74-75 0.6731015405282541 1105 76-77 0.3727396393487368 1105 78-79 0.5145771324098121 1105 80-81 0.3887691339280437 1105 82-83 0.5189807846673666 1105 84-85 0.6579322546982418 1105 86-87 0.6036733712588536 1105 88-89 0.4735921496622204 1105 90-91 0.47121022624656206 1105 92-93 0.4005412535211512 1105 94-95 0.3821462887506222 1105 96-97 0.6488901365669975 1105 98-99 0.7786718715751668 1105 100-101 1.1563222314368637 1106 1 0.18093605158470183 1106 2 0.1825608816272286 1106 3 0.28457501843971755 1106 4 0.2371767762272441 1106 5 0.28475315973566495 1106 6 0.20352969316461866 1106 7 0.3156634095042108 1106 8 0.3507568879100589 1106 9 0.5780606146307505 1106 10-11 0.43581679755661185 1106 12-13 0.553158796047633 1106 14-15 0.7230574787371324 1106 16-17 0.8222460668903793 1106 18-19 0.7075873513441877 1106 20-21 0.49351397370860184 1106 22-23 0.5534301699573021 1106 24-25 0.525046313838331 1106 26-27 0.2429729073042992 1106 28-29 0.498267175860839 1106 30-31 0.5130280014191442 1106 32-33 0.2903466483408792 1106 34-35 0.27848127274318557 1106 36-37 0.39618859829693065 1106 38-39 0.3666586896486166 1106 40-41 0.2963663737829947 1106 42-43 0.1244080917712509 1106 44-45 0.4031751953800935 1106 46-47 0.3679975252928358 1106 48-49 0.42926267802752704 1106 50-51 0.154103739551104 1106 52-53 0.3513990199724262 1106 54-55 0.4390992507012186 1106 56-57 0.37874433465982094 1106 58-59 0.4192329298622397 1106 60-61 0.4764697992322837 1106 62-63 0.5294634921409838 1106 64-65 0.39762435442938227 1106 66-67 0.5280554935473489 1106 68-69 0.43717296796165783 1106 70-71 0.5209497840428519 1106 72-73 0.6646243092839477 1106 74-75 0.47262942011648335 1106 76-77 0.6283499696350141 1106 78-79 0.6732315127532971 1106 80-81 0.6015461899402261 1106 82-83 0.8406201702210758 1106 84-85 0.6938042655751495 1106 86-87 0.7260132187508894 1106 88-89 0.7931267991656696 1106 90-91 0.8090615129621384 1106 92-93 0.972250975247043 1106 94-95 1.1255496567036793 1106 96-97 1.1006709602331348 1106 98-99 1.5072452869607993 1106 100-101 1.6451980426254842 1107 1 0.03414180072024564 1107 2 0.05220693012153177 1107 3 -0.046459824656690074 1107 4 0.12075191913796601 1107 5 0.16974720642003405 1107 6 0.20623027741419264 1107 7 0.3138975427582622 1107 8 0.24424557735411412 1107 9 0.12952915958314293 1107 10-11 0.12746977210866106 1107 12-13 0.08609010974409159 1107 14-15 -0.0769794023483712 1107 16-17 0.0339194185443219 1107 18-19 0.24862985421532358 1107 20-21 0.3286301407606729 1107 22-23 0.32691922384264416 1107 24-25 0.26686123698353015 1107 26-27 0.17515573113106342 1107 28-29 0.21312831955008704 1107 30-31 0.25153287735524543 1107 32-33 0.13336840204532763 1107 34-35 0.2830525006062814 1107 36-37 0.27616479061259724 1107 38-39 0.35391988647935335 1107 40-41 0.048764829704801116 1107 42-43 0.1882046532120043 1107 44-45 0.13096466100040516 1107 46-47 0.1799396895775658 1107 48-49 0.16614179257653916 1107 50-51 0.09769901412367687 1107 52-53 0.23645712278968745 1107 54-55 0.17511156212261625 1107 56-57 0.08404851715341266 1107 58-59 0.16532255396802498 1107 60-61 0.15507916940802602 1107 62-63 0.2598222687748333 1107 64-65 0.3155490175373572 1107 66-67 0.15807126771870372 1107 68-69 0.23795072469729206 1107 70-71 0.1736116324611281 1107 72-73 0.17331916232253874 1107 74-75 0.20897168105253527 1107 76-77 0.24325774693275903 1107 78-79 0.3769236004077463 1107 80-81 0.5110197201146143 1107 82-83 0.5129924347150769 1107 84-85 0.39137722611480896 1107 86-87 0.4393265096732968 1107 88-89 0.4110508393765606 1107 90-91 0.42885847454454407 1107 92-93 0.504262307139534 1107 94-95 0.4534645756559321 1107 96-97 0.6291295939632349 1107 98-99 0.9428632952315716 1107 100-101 1.313644475010335 1108 1 0.009681668845736624 1108 2 0.1866074735675518 1108 3 0.09429038840012893 1108 4 0.20090964444748494 1108 5 0.3649044410873259 1108 6 0.17398791308660577 1108 7 0.008956508394469154 1108 8 0.17924855635378378 1108 9 0.14996405698268234 1108 10-11 -0.06058524055384851 1108 12-13 0.21552096756946781 1108 14-15 0.1940510841072367 1108 16-17 0.2048527044161048 1108 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-2.0511477016827904 2316 86-87 -2.953594551211946 2316 88-89 -3.408681489003886 2316 90-91 -3.4550381028655828 2316 92-93 -4.0048508774764535 2316 94-95 -4.1953596033276135 2316 96-97 -5.239615913805874 2316 98-99 -6.386521775533314 2316 100-101 -6.837072485039847 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7149.0 3 2966.0 4 2968.0 5 3324.0 6 3702.0 7 4372.0 8 5416.0 9 6826.0 10 8807.0 11 10416.0 12 12994.0 13 15187.0 14 17449.0 15 20120.0 16 23235.0 17 27754.0 18 24786.0 19 20009.0 20 21078.0 21 23094.0 22 25457.0 23 29275.0 24 33897.0 25 38622.0 26 45170.0 27 52526.0 28 63037.0 29 74857.0 30 89932.0 31 108929.0 32 134106.0 33 169787.0 34 218143.0 35 290140.0 36 392643.0 37 498620.0 38 378064.0 39 52293.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.607289780390516 18.47166767418409 14.277339382722772 22.643703162702618 2 15.340693075870876 20.192501532246173 39.85740978012775 24.609395611755204 3 20.0887271649824 24.19760126826539 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26.959428921863278 27.601636511721534 22.362425090294064 48-49 23.07743683042961 26.862842901201166 27.504277810842982 22.555442457526244 50-51 22.34113189860563 27.19954861891354 27.9474655719627 22.511853910518127 52-53 22.974751136608045 27.424194567277322 26.96273711179137 22.638317184323263 54-55 22.4659165886862 26.903904276621383 27.426116958190182 23.204062176502237 56-57 22.99748733605874 27.031464613513457 27.0745374029763 22.8965106474515 58-59 23.49470028779312 26.720979676925634 27.032616046034725 22.751703989246526 60-61 22.93228950534174 26.831797386980355 27.984543472371737 22.25136963530617 62-63 22.58417481943516 27.032341541394896 27.680166629973712 22.703317009196237 64-65 22.435700499550666 27.477062009717102 27.27692048867874 22.810317002053495 66-67 22.678539602512206 27.161750347743478 26.84031801999445 23.319392029749864 68-69 22.528168116124377 27.01932238232605 27.330589662598175 23.1219198389514 70-71 22.968672350264466 26.93525862545627 27.077092115846916 23.018976908432347 72-73 22.81775121855789 26.622516153173326 27.715350422367457 22.844382205901326 74-75 22.441259159722634 27.65988098553433 27.09578562719318 22.803074227549853 76-77 22.76854144159667 26.98782072949818 27.04798406331117 23.195653765593978 78-79 22.791843873774432 27.103962405038633 27.167540527671363 22.93665319351557 80-81 22.99313532254988 27.17628334769534 27.001639568247025 22.828941761507753 82-83 22.862388566421522 27.09926987872232 27.29460467109202 22.743736883764132 84-85 22.62095617680802 27.362115640626016 27.089641237526248 22.92728694503972 86-87 22.646666983427703 27.395224945709586 27.048033429674906 22.910074641187805 88-89 22.610919885801405 27.23906087142377 26.631594539516 23.518424703258827 90-91 22.704355840886944 26.9812323482062 26.979051105171592 23.335360705735265 92-93 23.090247625741146 27.141255190190144 27.115567416538834 22.652929767529876 94-95 22.625605554120067 27.00979370913651 27.190800447511425 23.173800289231995 96-97 22.202509084821543 26.99435718017962 27.70277879706648 23.100354937932362 98-99 22.340684968805864 27.3238490978307 26.839862201228826 23.49560373213461 100-101 22.283525378593364 26.962620015601775 27.085333452410186 23.66852115339467 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 175.0 1 140.5 2 135.5 3 205.5 4 208.0 5 196.5 6 366.0 7 774.5 8 1258.0 9 1572.5 10 1602.5 11 1547.5 12 1689.0 13 1919.0 14 2123.0 15 2308.5 16 2463.5 17 2683.5 18 2997.5 19 3337.0 20 3713.5 21 4374.0 22 5243.5 23 6381.0 24 7865.5 25 10015.5 26 13135.5 27 17079.5 28 21148.5 29 25884.5 30 31674.0 31 37943.5 32 44121.0 33 49195.0 34 55074.5 35 60922.5 36 67166.0 37 73828.5 38 78004.5 39 80103.0 40 83128.0 41 88737.0 42 93345.5 43 98008.5 44 102771.0 45 106700.5 46 110017.0 47 122236.5 48 150332.5 49 148609.5 50 121301.0 51 108764.5 52 102614.5 53 100311.5 54 96887.5 55 88991.5 56 81022.0 57 73291.5 58 67119.5 59 59910.5 60 50333.0 61 41118.0 62 32947.5 63 25315.0 64 18706.5 65 14401.5 66 11025.0 67 8580.5 68 6636.5 69 4929.0 70 3744.5 71 2984.0 72 2551.0 73 2364.5 74 1932.0 75 1519.5 76 1165.5 77 762.5 78 495.5 79 330.5 80 229.0 81 168.0 82 102.5 83 67.5 84 46.5 85 25.5 86 19.0 87 18.0 88 12.0 89 9.0 90 7.0 91 5.0 92 3.5 93 2.5 94 3.5 95 3.0 96 3.0 97 2.5 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.035473224309810765 2 0.024212420024618216 3 0.023028852006655033 4 0.051806462957702665 5 0.05799483288019586 6 0.05072434362699347 7 0.05231370639397259 8 0.04402873026823033 9 0.04497558468260088 10-11 0.05386925293186706 12-13 0.04784996415479717 14-15 0.04289588659389414 16-17 0.0515528412395677 18-19 0.05532335078250754 20-21 0.012697994021290697 22-23 0.02208199759228449 24-25 0.0064419916406281705 26-27 0.011734231492377822 28-29 0.006797062046017124 30-31 0.01039849044353366 32-33 0.0037536014283975168 34-35 0.0090627493946895 36-37 0.010330857985364336 38-39 0.012562729104952049 40-41 0.002079698088706732 42-43 0.002434768494095686 44-45 0.0025700334104343356 46-47 5.579677798969281E-4 48-49 0.0013526491633864925 50-51 4.5651909264294124E-4 52-53 0.003922682573820828 54-55 0.007541019085879696 56-57 0.01815931501846366 58-59 0.011784955836004815 60-61 0.01186949640871647 62-63 0.006391267297001177 64-65 8.454057271165578E-5 66-67 0.008082078751234293 68-69 0.01815931501846366 70-71 0.005799483288019586 72-73 0.002654573983145991 74-75 0.004210120521040458 76-77 0.007084499993236754 78-79 0.005681126486223268 80-81 0.009603809060044096 82-83 0.0062729104952048585 84-85 0.018666558454733598 86-87 0.004954077560903028 88-89 0.006966143191440436 90-91 0.004413017895548431 92-93 0.016925022656873488 94-95 0.012309107386817082 96-97 0.010313949870822006 98-99 0.02282595463214706 100-101 0.036724424785943274 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2957160.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.799479170718975 #Duplication Level Percentage of deduplicated Percentage of total 1 74.11012652274846 38.38865955154457 2 14.009904947030854 14.514115589749549 3 5.0546090321014745 7.854783458234051 4 2.367873910708357 4.906185412665058 5 1.2665708399838598 3.280385492199199 6 0.7459522528228621 2.3183962909469247 7 0.48591338899152214 1.761904233028787 8 0.32621202188851267 1.351809026644171 9 0.24973592529988525 1.1642571774676473 >10 1.2279167315296369 11.58869678228291 >50 0.09711661164068595 3.493569276288784 >100 0.05361314988731971 5.022013474482522 >500 0.0025548816073382163 0.8455266839753519 >1k 0.0015067250503869656 1.7633661408055754 >5k 3.275489239971664E-4 1.2374951683421096 >10k+ 6.55097847994333E-5 0.5088362413428194 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 14995 0.5070743551245114 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 9524 0.3220657658023238 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7598 0.2569357085852642 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7260 0.2455058231546484 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6236 0.21087800457195416 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5850 0.19782494014527452 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4653 0.15734691393093372 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4020 0.1359412409203425 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3841 0.12988813591418794 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3604 0.12187368962112298 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3510 0.1186949640871647 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3270 0.11057906910684576 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2969 0.10040038415236241 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 1.6908114542331157E-5 0.0 1.6908114542331157E-5 0.0 14-15 0.0 3.3816229084662314E-5 0.0 3.3816229084662314E-5 0.0 16-17 0.0 3.3816229084662314E-5 0.0 1.0144868725398694E-4 0.0 18-19 1.6908114542331157E-5 3.3816229084662314E-5 0.0 1.0144868725398694E-4 6.763245816932463E-5 20-21 3.3816229084662314E-5 3.3816229084662314E-5 0.0 1.0144868725398694E-4 8.454057271165578E-5 22-23 3.3816229084662314E-5 3.3816229084662314E-5 0.0 1.3526491633864926E-4 1.3526491633864926E-4 24-25 6.763245816932463E-5 3.3816229084662314E-5 0.0 2.0289737450797387E-4 1.3526491633864926E-4 26-27 6.763245816932463E-5 3.3816229084662314E-5 0.0 2.8743794721962964E-4 1.3526491633864926E-4 28-29 6.763245816932463E-5 3.3816229084662314E-5 0.0 5.579677798969282E-4 1.3526491633864926E-4 30-31 6.763245816932463E-5 3.3816229084662314E-5 0.0 0.0016569952251484533 1.3526491633864926E-4 32-33 6.763245816932463E-5 3.3816229084662314E-5 0.0 0.003026552503077277 1.3526491633864926E-4 34-35 6.763245816932463E-5 3.3816229084662314E-5 0.0 0.004294661093752114 1.3526491633864926E-4 36-37 6.763245816932463E-5 3.3816229084662314E-5 0.0 0.006154553693408541 1.3526491633864926E-4 38-39 6.763245816932463E-5 3.3816229084662314E-5 0.0 0.00994197135089072 1.3526491633864926E-4 40-41 6.763245816932463E-5 3.3816229084662314E-5 0.0 0.013441951061153269 1.3526491633864926E-4 42-43 6.763245816932463E-5 3.3816229084662314E-5 0.0 0.017060287573212138 1.3526491633864926E-4 44-45 1.0144868725398694E-4 3.3816229084662314E-5 0.0 0.0188863639437839 1.3526491633864926E-4 46-47 1.3526491633864926E-4 3.3816229084662314E-5 0.0 0.02181146775960719 1.3526491633864926E-4 48-49 1.3526491633864926E-4 3.3816229084662314E-5 0.0 0.026123036967901636 1.3526491633864926E-4 50-51 1.3526491633864926E-4 3.3816229084662314E-5 0.0 0.031685806652328584 1.3526491633864926E-4 52-53 1.3526491633864926E-4 3.3816229084662314E-5 0.0 0.04233791881399722 1.3526491633864926E-4 54-55 1.3526491633864926E-4 3.3816229084662314E-5 0.0 0.050791976085162796 1.3526491633864926E-4 56-57 1.3526491633864926E-4 3.3816229084662314E-5 0.0 0.07089572427599453 1.521730308809804E-4 58-59 1.3526491633864926E-4 3.3816229084662314E-5 0.0 0.10347766099906666 1.6908114542331157E-4 60-61 1.521730308809804E-4 3.3816229084662314E-5 0.0 0.1281296920017855 1.8598925996564272E-4 62-63 1.6908114542331157E-4 3.3816229084662314E-5 0.0 0.1419098053537854 2.705298326772985E-4 64-65 1.6908114542331157E-4 3.3816229084662314E-5 0.0 0.1661729497220306 2.705298326772985E-4 66-67 1.6908114542331157E-4 3.3816229084662314E-5 0.0 0.22437067997673443 2.705298326772985E-4 68-69 1.6908114542331157E-4 3.3816229084662314E-5 0.0 0.3048702133127731 2.705298326772985E-4 70-71 2.0289737450797387E-4 3.3816229084662314E-5 0.0 0.37676351634676514 2.705298326772985E-4 72-73 2.3671360359263618E-4 3.3816229084662314E-5 0.0 0.4575843038591081 2.8743794721962964E-4 74-75 2.3671360359263618E-4 3.3816229084662314E-5 0.0 0.5532335078250754 3.043460617619608E-4 76-77 2.3671360359263618E-4 3.3816229084662314E-5 0.0 0.6154891855699387 3.043460617619608E-4 78-79 2.3671360359263618E-4 3.3816229084662314E-5 0.0 0.6985925685454963 3.3816229084662313E-4 80-81 2.3671360359263618E-4 3.3816229084662314E-5 0.0 0.8112513357410489 3.3816229084662313E-4 82-83 2.3671360359263618E-4 3.3816229084662314E-5 0.0 0.9086927998485033 3.7197851993128544E-4 84-85 2.705298326772985E-4 3.3816229084662314E-5 0.0 1.045107467976031 3.7197851993128544E-4 86-87 3.043460617619608E-4 3.3816229084662314E-5 0.0 1.2574226622840834 3.7197851993128544E-4 88-89 3.043460617619608E-4 3.3816229084662314E-5 0.0 1.5358824006817353 3.7197851993128544E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 13020 0.0 24.454277 1 TACATGG 13200 0.0 23.365969 2 ACATGGG 13305 0.0 22.145721 3 GAGTACT 8715 0.0 21.320286 12-13 AGTACTT 9275 0.0 20.237963 12-13 CATGGGG 6130 0.0 18.99474 4 GTACTTT 9760 0.0 18.93983 14-15 AGAGTAC 17650 0.0 18.388287 10-11 ATTCGCG 195 1.4551915E-11 18.269806 80-81 TAGCCCT 1760 0.0 17.822105 4 ACCGTCG 305 9.246469E-8 17.138065 8 TACTTTT 11010 0.0 16.703209 14-15 GTATAGC 1035 0.0 16.52925 1 ACTTTTT 11550 0.0 16.517733 16-17 CATGGGA 7095 0.0 16.009335 4 AGTACAT 10105 0.0 15.9432335 2 GTGTAGC 1865 0.0 15.798062 1 GAGTACA 12090 0.0 15.486772 1 GTATCAA 32085 0.0 15.033322 1 GTACCTG 2150 0.0 15.030086 1 >>END_MODULE