FastQCFastQC Report
Thu 26 May 2016
SRR936433_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936433_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2957160
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT168740.5706150495745919No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91180.308336376793951No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT84440.28554423839088855No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81530.27570371572725183No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT67780.22920640073584114No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC57340.19390225757145368No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT51170.17303764422621706No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46830.1583614008034736No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC44590.15078656548850924No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC42740.1445305631078467No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT40900.13830837695626885No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG35860.12126499749759904No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA34090.11527952494961383No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC30910.1045259641006912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG126550.022.3430211
GAGTACT107950.021.5390312-13
TACATGG128550.021.4318332
ACATGGG127650.020.4662343
GTATAGC10800.020.2404961
AGTACTT112450.019.7900212-13
GTACTTT117650.019.60135314-15
GTGTAGC19400.017.8816741
TAGCCCT20400.017.6946834
AGAGTAC200900.017.52030610-11
GTGTAGG14250.017.0075741
ACTTTTT138400.016.85004616-17
TACTTTT133900.016.61949214-15
GTATAAC8100.016.4270691
CATGGGA67650.016.358654
GGACCGT3854.66207E-916.0379686
AGTACAT101050.016.0295562
TAAGGTG10800.015.8323545
ATAGCCC10250.015.7561873
CATGGGG59550.015.4731714
Produced by FastQC (version 0.11.5)