##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936433_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2957160 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.850131545131138 31.0 31.0 34.0 30.0 34.0 2 31.99945150076425 31.0 31.0 34.0 30.0 34.0 3 32.11056993872499 33.0 31.0 34.0 30.0 34.0 4 35.85318379796832 37.0 35.0 37.0 35.0 37.0 5 35.63002881142718 37.0 35.0 37.0 33.0 37.0 6 35.723947300788595 37.0 35.0 37.0 33.0 37.0 7 35.67460130665909 37.0 35.0 37.0 33.0 37.0 8 35.647021804704515 37.0 35.0 37.0 33.0 37.0 9 37.28425854536109 39.0 37.0 39.0 34.0 39.0 10-11 37.14402112161669 39.0 37.0 39.0 33.0 39.0 12-13 37.054856348658845 39.0 37.0 39.0 33.0 39.0 14-15 38.14711327760419 40.0 37.0 41.0 33.0 41.0 16-17 38.080500716904055 40.0 37.0 41.0 33.0 41.0 18-19 38.23510614914309 40.0 37.5 41.0 33.5 41.0 20-21 38.27020249157976 40.0 38.0 41.0 33.5 41.0 22-23 38.18206065955173 40.0 37.5 41.0 33.0 41.0 24-25 38.05683933233237 40.0 37.0 41.0 33.0 41.0 26-27 37.84558850383476 40.0 37.0 41.0 33.0 41.0 28-29 37.77093427477715 40.0 37.0 41.0 32.5 41.0 30-31 37.755411780221564 40.0 37.0 41.0 33.0 41.0 32-33 37.619533099325025 40.0 37.0 41.0 32.5 41.0 34-35 37.450507750679705 40.0 36.0 41.0 32.0 41.0 36-37 37.202225784198355 39.0 36.0 41.0 31.5 41.0 38-39 37.04795479446496 39.0 36.0 41.0 31.0 41.0 40-41 36.86129681856917 39.0 35.5 41.0 31.0 41.0 42-43 36.62569120372249 39.0 35.0 40.5 30.0 41.0 44-45 36.391468672645374 39.0 35.0 40.0 30.0 41.0 46-47 36.070835869550514 39.0 35.0 40.0 29.5 41.0 48-49 35.72849981062912 39.0 35.0 40.0 28.5 41.0 50-51 35.41718878924374 38.0 34.5 40.0 27.5 41.0 52-53 35.15928289304603 38.0 34.0 40.0 27.0 41.0 54-55 34.90619851479122 38.0 34.0 40.0 26.0 41.0 56-57 34.57829894222836 38.0 34.0 40.0 26.0 41.0 58-59 34.247008278212874 37.5 33.0 40.0 25.5 41.0 60-61 33.86849697006588 37.0 33.0 40.0 24.5 41.0 62-63 33.485673247304845 36.0 32.0 39.0 23.0 40.5 64-65 33.07958176087868 36.0 32.0 39.0 22.5 40.0 66-67 32.60120351959313 35.5 31.0 38.5 22.0 40.0 68-69 32.31116882414209 35.0 31.0 38.0 21.5 40.0 70-71 31.871480068714575 35.0 31.0 37.5 20.0 40.0 72-73 31.413670379688618 34.5 30.5 37.0 19.0 39.0 74-75 30.892114055377455 34.0 30.0 36.5 18.0 39.0 76-77 29.464071947409 32.5 28.5 35.0 16.0 37.0 78-79 30.24835450229274 34.0 29.5 35.0 16.5 37.5 80-81 30.03398852277185 34.0 29.0 35.0 12.5 37.0 82-83 29.692576323229044 34.0 29.0 35.0 8.5 36.5 84-85 29.273539815228126 34.0 29.0 35.0 7.0 36.0 86-87 28.90943134629171 34.0 29.0 35.0 3.5 36.0 88-89 28.500990646431035 33.0 28.5 35.0 2.0 35.0 90-91 27.995925989801023 33.0 27.0 35.0 2.0 35.0 92-93 27.494568944527856 33.0 26.5 35.0 2.0 35.0 94-95 26.889066874974638 32.0 25.5 34.0 2.0 35.0 96-97 26.114136367325408 32.0 24.5 34.0 2.0 35.0 98-99 25.154538645186598 32.0 21.0 34.0 2.0 35.0 100-101 21.878712007466625 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3658621298122746 1101 2 0.4093712805614338 1101 3 0.3187031210456297 1101 4 0.21323418650982973 1101 5 0.22114122390262025 1101 6 0.2796356052888811 1101 7 0.26797640674670475 1101 8 0.2946675374351386 1101 9 0.38023804368810943 1101 10-11 0.3855926686806441 1101 12-13 0.32041117369568184 1101 14-15 0.5601789040153093 1101 16-17 0.43305977306339116 1101 18-19 0.43959121615242935 1101 20-21 0.3723578552752187 1101 22-23 0.355399963220826 1101 24-25 0.29456961050887287 1101 26-27 0.3731224604849004 1101 28-29 0.268341886512502 1101 30-31 0.3741058436051006 1101 32-33 0.42109859268325067 1101 34-35 0.34005673844436757 1101 36-37 0.2689759503802662 1101 38-39 0.2899312099234521 1101 40-41 0.39928042679259335 1101 42-43 0.3202102896723815 1101 44-45 0.3323453824500362 1101 46-47 0.2645391464216118 1101 48-49 0.24006488561492034 1101 50-51 0.2866000999622571 1101 52-53 0.31701731622495544 1101 54-55 0.24178243799532595 1101 56-57 0.12085623932317446 1101 58-59 0.1780948900141368 1101 60-61 0.09258779503722536 1101 62-63 0.06904378549086232 1101 64-65 0.1405608789367676 1101 66-67 0.21735549017331834 1101 68-69 0.08601504106513147 1101 70-71 0.13095361628040436 1101 72-73 0.19731552731252222 1101 74-75 0.056206102052009044 1101 76-77 -0.06192325227633688 1101 78-79 -0.15881232564744607 1101 80-81 0.011137230316357716 1101 82-83 0.2009229814267499 1101 84-85 0.24976799584861098 1101 86-87 0.15866946932411352 1101 88-89 -0.02169224255935731 1101 90-91 0.1095559828738999 1101 92-93 0.018179861585263524 1101 94-95 0.08547753631343014 1101 96-97 0.17089744219374126 1101 98-99 0.36645973599991066 1101 100-101 0.686683304477171 1102 1 0.19832534665682644 1102 2 0.24112593381710568 1102 3 0.19310864352417667 1102 4 0.07421133957623027 1102 5 0.017400927870234284 1102 6 0.15357223277520404 1102 7 0.1299698671174454 1102 8 0.19464570921859092 1102 9 0.19110116591026127 1102 10-11 0.22148820690883753 1102 12-13 0.22038126281384507 1102 14-15 0.3605661389867194 1102 16-17 0.3327698614144552 1102 18-19 0.20479897679629033 1102 20-21 0.13480726833315515 1102 22-23 0.13391997399235578 1102 24-25 0.2380392487046734 1102 26-27 0.04207576838344096 1102 28-29 0.015331922042086887 1102 30-31 0.2543736579499978 1102 32-33 0.0948461259642599 1102 34-35 0.09601421499007756 1102 36-37 0.2745676486697306 1102 38-39 0.23441750684383322 1102 40-41 0.23239020665693744 1102 42-43 0.12547463499758038 1102 44-45 0.12031111717033838 1102 46-47 -0.008408293361526376 1102 48-49 -0.008283891131419807 1102 50-51 -0.2070229783464228 1102 52-53 -0.16311810305852958 1102 54-55 -0.0033132918874301254 1102 56-57 -0.17526841887045208 1102 58-59 -0.30678992347107936 1102 60-61 -0.13839409517387224 1102 62-63 -0.10118271789994537 1102 64-65 0.03640253339911226 1102 66-67 0.11907951765799396 1102 68-69 -0.0017313271072012526 1102 70-71 0.04649142076344148 1102 72-73 -0.02001900848042837 1102 74-75 0.013679083358663746 1102 76-77 -0.2964762748618135 1102 78-79 -0.16141810665079248 1102 80-81 0.22723270858222122 1102 82-83 0.06790244221959796 1102 84-85 0.05052648119127667 1102 86-87 0.12767159451285437 1102 88-89 0.01609627595938079 1102 90-91 0.18081095270899183 1102 92-93 0.272137606415221 1102 94-95 0.37508214437646004 1102 96-97 0.4753486011073704 1102 98-99 0.39620429566333115 1102 100-101 1.1276856212401398 1103 1 0.17291812528738504 1103 2 0.1805749543386419 1103 3 0.1975411947366439 1103 4 0.1635344989424894 1103 5 0.005276512633500374 1103 6 0.18413153827746243 1103 7 0.060730527625828756 1103 8 0.14471669171224022 1103 9 0.17343320636856419 1103 10-11 0.07709907893705292 1103 12-13 0.12875831062738285 1103 14-15 0.12520563586189581 1103 16-17 0.2459233800446441 1103 18-19 0.13853641923365245 1103 20-21 0.13980930700976302 1103 22-23 0.13663649629558705 1103 24-25 0.2220754686213411 1103 26-27 0.14485976117440202 1103 28-29 0.15962339911754242 1103 30-31 0.08836799077166546 1103 32-33 0.19349408934346002 1103 34-35 0.1882861744495372 1103 36-37 0.2346437078678747 1103 38-39 0.2499832595894773 1103 40-41 0.29639480138858687 1103 42-43 0.14620246538882498 1103 44-45 0.15023383590834527 1103 46-47 0.005262118302674423 1103 48-49 0.0022305691960511353 1103 50-51 -0.06316793225062867 1103 52-53 -0.15325107959490936 1103 54-55 -0.094801474415803 1103 56-57 -0.16150478200843565 1103 58-59 0.14228970255454243 1103 60-61 0.03935808969355037 1103 62-63 -0.02335952198598079 1103 64-65 -0.08337557064306367 1103 66-67 0.03613107733125531 1103 68-69 0.020010273773600318 1103 70-71 -0.021320274044956733 1103 72-73 -0.10665990901585332 1103 74-75 0.02158095391583359 1103 76-77 -0.1187233761720492 1103 78-79 -0.09732571177112703 1103 80-81 0.005797594462016775 1103 82-83 0.1355545263524114 1103 84-85 -0.05017914646985133 1103 86-87 -0.11561476359982237 1103 88-89 -0.13216648033139222 1103 90-91 -0.02152770059949205 1103 92-93 0.15914995154432532 1103 94-95 0.23463888354463336 1103 96-97 0.3443160884984131 1103 98-99 0.8585176168445514 1103 100-101 1.1482082990904061 1104 1 0.13113056566894343 1104 2 0.1659783724626429 1104 3 0.20050224402122296 1104 4 0.098763477921473 1104 5 -0.01007137201585806 1104 6 0.06053091559595458 1104 7 0.05315512133718414 1104 8 0.006460972757992067 1104 9 -0.016540958973244813 1104 10-11 0.13119619075474986 1104 12-13 0.06805652428221265 1104 14-15 0.07136659461476569 1104 16-17 0.11743901332499007 1104 18-19 0.0386448916766895 1104 20-21 0.0016758610638092364 1104 22-23 -0.05147404071200157 1104 24-25 0.0074603939878841175 1104 26-27 0.07046450194458487 1104 28-29 0.08938183402303679 1104 30-31 -0.06990406805186922 1104 32-33 -0.011599975309430022 1104 34-35 -0.0032350342591698222 1104 36-37 0.1221445359017963 1104 38-39 0.07306304893764803 1104 40-41 0.013445399781097933 1104 42-43 -0.028123791014635913 1104 44-45 0.009324268252463241 1104 46-47 0.08900808853761077 1104 48-49 0.0366472469031649 1104 50-51 -0.05426849972987213 1104 52-53 0.003020449286914584 1104 54-55 0.02530843328602117 1104 56-57 0.11960058806752016 1104 58-59 -0.12634050853890955 1104 60-61 0.01507489105373594 1104 62-63 0.07872822752824504 1104 64-65 0.256455344831231 1104 66-67 -0.0572071138010557 1104 68-69 0.004388585909268272 1104 70-71 -0.04493966549523165 1104 72-73 -0.14371166665584667 1104 74-75 -0.024283016672402624 1104 76-77 -0.04325581302066439 1104 78-79 -0.165736217865458 1104 80-81 0.09628624487713822 1104 82-83 0.11772640411252411 1104 84-85 0.16177738726976543 1104 86-87 0.15187165664395152 1104 88-89 0.19243468333226943 1104 90-91 0.23109054264375217 1104 92-93 0.4198594662060451 1104 94-95 0.3110871148641863 1104 96-97 0.37983239524633916 1104 98-99 0.5440452400560005 1104 100-101 0.9354830738651785 1105 1 0.08263935601268813 1105 2 0.12243555780332471 1105 3 0.07412957280632071 1105 4 0.04419792936320022 1105 5 -0.04294295719326868 1105 6 -6.051597998322222E-4 1105 7 0.020869376854840027 1105 8 0.046597164775249666 1105 9 -0.06681046391508261 1105 10-11 -0.058052858051340195 1105 12-13 -0.12219501366350016 1105 14-15 -0.02091771032123546 1105 16-17 -0.005852390943353214 1105 18-19 -0.052739696795278235 1105 20-21 0.043758538401341696 1105 22-23 -0.012070195118838 1105 24-25 0.04130822847285742 1105 26-27 0.053571152284249024 1105 28-29 -0.05064037872369198 1105 30-31 0.08232085104198461 1105 32-33 0.0893006522598867 1105 34-35 0.11676008221652268 1105 36-37 -0.026241441094619233 1105 38-39 0.1362015515178996 1105 40-41 0.12999435164558548 1105 42-43 0.05268596291404748 1105 44-45 0.20231568199629635 1105 46-47 0.21885881304853427 1105 48-49 0.3550155849013592 1105 50-51 0.35329795628926064 1105 52-53 0.3129765522345238 1105 54-55 0.34726288166643826 1105 56-57 0.23631253666660967 1105 58-59 0.2521554935311272 1105 60-61 0.3861346340491565 1105 62-63 0.37118883133592817 1105 64-65 0.26729458212678026 1105 66-67 0.48234633527908954 1105 68-69 0.4073960533281422 1105 70-71 0.25713073014312116 1105 72-73 0.22438306642674988 1105 74-75 -0.05963900372741904 1105 76-77 -0.11363997145093663 1105 78-79 0.24118918447863535 1105 80-81 0.36970274390042945 1105 82-83 0.3120965023661171 1105 84-85 0.3250097336938609 1105 86-87 0.2850699641747809 1105 88-89 0.38788307468479033 1105 90-91 0.5271171214069845 1105 92-93 0.43060941518415063 1105 94-95 0.3263171507252913 1105 96-97 0.42330815255704834 1105 98-99 0.5820981898504556 1105 100-101 0.9869016647861955 1106 1 0.03296803567839035 1106 2 0.12101776075288129 1106 3 0.10612751048781632 1106 4 0.0597061403488226 1106 5 -0.03855011367121364 1106 6 -0.022491017565165805 1106 7 0.10671000625612947 1106 8 0.013138637122011687 1106 9 0.007450926509513067 1106 10-11 0.08738155954451088 1106 12-13 8.479609400282584E-4 1106 14-15 0.15036898085500638 1106 16-17 0.15561388785928187 1106 18-19 0.05535447165568286 1106 20-21 0.060061233754787224 1106 22-23 0.09961839103306858 1106 24-25 0.06789469157034489 1106 26-27 0.008424995689182424 1106 28-29 0.100578121557092 1106 30-31 0.166496591809981 1106 32-33 0.09934361597749586 1106 34-35 0.13218261351541827 1106 36-37 -0.07159081714380022 1106 38-39 0.13823778135642328 1106 40-41 0.17848605132035544 1106 42-43 0.23666258521621586 1106 44-45 0.16564121000088505 1106 46-47 0.011419751669969003 1106 48-49 0.009033830827029021 1106 50-51 0.04384549734793808 1106 52-53 -0.07597933614652419 1106 54-55 -0.03784361452423468 1106 56-57 -0.01616649667884218 1106 58-59 -0.06525888871927776 1106 60-61 -0.08123656113460953 1106 62-63 -0.25051282917956286 1106 64-65 -0.09151017404365547 1106 66-67 0.03029777432245595 1106 68-69 -0.020248671205564506 1106 70-71 -0.020895067231638365 1106 72-73 -0.03982615417151081 1106 74-75 -0.013900503011200271 1106 76-77 -0.34671790486815723 1106 78-79 -0.17307383695686696 1106 80-81 -0.06300820267898999 1106 82-83 0.14709225310410545 1106 84-85 -0.10302092480661429 1106 86-87 0.002557572996430224 1106 88-89 -0.02420351378844643 1106 90-91 0.15050839848288788 1106 92-93 0.08943778253660639 1106 94-95 0.3622891055960231 1106 96-97 0.2738456166335155 1106 98-99 0.677899106294646 1106 100-101 0.89090728867696 1107 1 -0.042621455062334945 1107 2 0.03417307753742094 1107 3 -0.0027065554003797843 1107 4 -0.036843093483113876 1107 5 -0.024552128696797126 1107 6 0.012541086271589563 1107 7 -0.04057214966691447 1107 8 -0.1330140297467679 1107 9 -0.01869857195141833 1107 10-11 -0.045745539072456154 1107 12-13 -0.118193296322886 1107 14-15 0.01610565287951715 1107 16-17 -0.07324775098666692 1107 18-19 -0.07141814701574134 1107 20-21 -0.09411248815104756 1107 22-23 -0.19217556279314607 1107 24-25 -0.05951225945906913 1107 26-27 -0.021294028452359726 1107 28-29 -0.13212172544265144 1107 30-31 -0.06838618407230257 1107 32-33 -0.061249850613052104 1107 34-35 -0.22075115272209445 1107 36-37 -0.04543481080288814 1107 38-39 -0.10626903337008287 1107 40-41 -0.07969684074617334 1107 42-43 -0.1454079823294876 1107 44-45 -0.04127511381671667 1107 46-47 0.07577822206157947 1107 48-49 -0.03239988860023146 1107 50-51 -0.04597612259168926 1107 52-53 -0.10249462394682496 1107 54-55 -0.1588533660245588 1107 56-57 -0.32040853737893116 1107 58-59 -0.15118121940306395 1107 60-61 0.02894570505980454 1107 62-63 0.08704565225684746 1107 64-65 -0.08604824983647319 1107 66-67 -0.05882396961087011 1107 68-69 0.033229600052351316 1107 70-71 0.05890209330594942 1107 72-73 -0.06937583397916569 1107 74-75 -0.08241346452331655 1107 76-77 -0.057298304565406966 1107 78-79 -0.12268225317037462 1107 80-81 0.21075116064953647 1107 82-83 0.09515202630820951 1107 84-85 0.06913555698140783 1107 86-87 0.04537282237234663 1107 88-89 -0.0071171678877455236 1107 90-91 0.12819896757003235 1107 92-93 0.05067680292960475 1107 94-95 0.3001568879736354 1107 96-97 0.3353557274152976 1107 98-99 0.5757887960556438 1107 100-101 0.7170954346479661 1108 1 0.04538317187665086 1108 2 0.07394068463449699 1108 3 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-1.1024270381000534 2316 98-99 -1.274956914272238 2316 100-101 -1.6219003965617702 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 10.0 4 36.0 5 70.0 6 198.0 7 507.0 8 1137.0 9 2132.0 10 3504.0 11 5318.0 12 7508.0 13 10454.0 14 13685.0 15 18103.0 16 22594.0 17 28028.0 18 25389.0 19 17996.0 20 17024.0 21 17907.0 22 19583.0 23 22371.0 24 25901.0 25 31608.0 26 38916.0 27 48575.0 28 60038.0 29 74458.0 30 91956.0 31 112913.0 32 140902.0 33 177730.0 34 232303.0 35 311222.0 36 436966.0 37 569661.0 38 350256.0 39 20198.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.0033652857313 19.37576453013196 15.008842839667883 22.61202734446886 2 15.650028660950257 20.93527697976148 39.09830452301922 24.316389836269035 3 20.21094771927024 24.15777149821646 29.930748222206287 25.70053256030701 4 13.061661204750665 17.066946035413235 37.30147645886587 32.56991630097023 5 13.687365072150893 37.67631924943594 33.98680344766017 14.649512230752999 6 31.65648457943009 36.486364930605575 17.06407579720231 14.793074692762026 7 28.43948247643009 32.876306997254126 20.372688660742064 18.311521865573727 8 26.3718229652775 33.95737802486169 19.529548620974175 20.141250388886633 9 25.945609789007047 17.157748114461636 20.617406233715112 36.2792358628162 10-11 25.234208044816175 26.30721880028602 26.935273582150902 21.523299572746904 12-13 26.61805303222571 23.72460224478053 27.41112022093069 22.24622450206307 14-15 23.065217255301928 25.363560383394795 26.476488772731877 25.094733588571405 16-17 22.059610771831565 28.542452139490166 26.998920896031663 22.399016192646602 18-19 21.871407025659757 27.40957540342761 29.205014270448675 21.51400330046396 20-21 23.082281648608802 25.87161330465717 29.034698832663775 22.011406214070256 22-23 22.402429801078043 25.939983047663524 29.147924437826127 22.5096627134323 24-25 22.842946688958506 26.057729505846194 29.173260884856155 21.92606292033914 26-27 22.469016229787165 25.98172762418533 29.599206231112817 21.950049914914686 28-29 21.896709036099352 26.915586238242327 29.26794872922138 21.919755996436937 30-31 22.18235807931144 26.12216181449089 29.09533476242312 22.60014534377455 32-33 22.223011269968968 26.258060545437147 29.102216964909804 22.416711219684082 34-35 23.101689042773177 26.119736759115042 28.798680650401607 21.97989354771017 36-37 22.75475519756118 25.91029927691472 29.186039098788964 22.14890642673513 38-39 22.38623700715103 26.359212219492274 29.293571171305228 21.960979602051463 40-41 22.52248520219816 26.399816171963757 28.955316700097377 22.12238192574071 42-43 22.264541490194702 26.541851691349493 28.37753776819949 22.816069050256317 44-45 22.7158089093141 26.210971448668136 28.289270339822732 22.783949302195033 46-47 23.056119582362598 26.818751852496238 27.945796615230467 22.1793319499107 48-49 23.15654385948597 26.736181972968836 27.78383185795071 22.323442309594483 50-51 22.426543380733357 26.971971026701 28.230519625996298 22.370965966569347 52-53 23.00611531185013 27.290266040667404 27.25817363075907 22.445445016723394 54-55 22.581919300987764 26.749111541189013 27.611937017747536 23.05703214007569 56-57 22.998939170185576 26.93219312445661 27.328186657351296 22.740681048006522 58-59 23.61918668548669 26.56306748358377 27.251335245545178 22.56641058538436 60-61 22.999977004233248 26.762304256989864 28.149523886176365 22.088194852600516 62-63 22.667635205718167 26.86517320920883 27.92716748657691 22.54002409849609 64-65 22.514283028091576 27.30826027163075 27.512256425058723 22.66520027521895 66-67 22.752009295692677 26.991190768830688 27.112713148173604 23.144086787303035 68-69 22.611845950864527 26.852260320563992 27.536045399000596 22.99984832957088 70-71 23.04912483599129 26.742279754899972 27.369536988191374 22.839058420917365 72-73 22.960143909974768 26.44229119045127 27.913127828653934 22.68443707092003 74-75 22.535809990950277 27.50742645576795 27.276042984163656 22.68072056911812 76-77 22.83094538044953 26.851623744272473 27.22563876337649 23.09179211190151 78-79 22.844560400261745 26.935846861668576 27.355452112144977 22.86414062592469 80-81 23.06093248387654 26.987680531831465 27.164259913757977 22.78712707053402 82-83 22.905488155942876 26.93873906990845 27.452328458455717 22.70344431569295 84-85 22.645972124220688 27.143080099620846 27.338564628510913 22.87238314764755 86-87 22.66271228855688 27.153304295482677 27.18674967704409 22.997233738916357 88-89 22.63570405910336 27.16418071971136 26.74707461797684 23.45304060320844 90-91 22.74959246758342 26.75700583735229 27.1042031642102 23.389198530854095 92-93 23.071780348855672 26.95901814109682 27.21416456623163 22.755036943815877 94-95 22.682302287313153 26.863281992388472 27.28459088551125 23.169824834787125 96-97 22.254051190607633 26.85977543387165 27.75650805368526 23.129665321835454 98-99 22.34892108044641 27.12625786022616 26.832570440426295 23.692250618901134 100-101 22.127111727195054 26.931630858478368 27.080902331512867 23.860355082813708 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 283.0 1 227.0 2 204.5 3 255.0 4 245.5 5 234.5 6 414.5 7 843.5 8 1349.5 9 1715.0 10 1738.5 11 1656.5 12 1789.0 13 2084.0 14 2379.5 15 2640.5 16 3017.0 17 3340.0 18 3587.5 19 3872.0 20 4291.5 21 5006.0 22 5856.0 23 7039.0 24 8665.5 25 10712.5 26 13732.0 27 17657.0 28 21700.5 29 26329.0 30 32233.0 31 38528.0 32 44341.0 33 49162.0 34 54910.5 35 61079.0 36 67220.5 37 73311.5 38 77211.5 39 79458.0 40 82849.5 41 88241.0 42 92758.0 43 97494.5 44 101659.0 45 105204.5 46 108457.5 47 122308.5 48 153363.5 49 150242.0 50 120493.5 51 107776.0 52 101441.0 53 99309.5 54 96515.0 55 89142.0 56 80653.5 57 72714.5 58 67079.5 59 60079.5 60 50489.0 61 40873.5 62 32170.0 63 24968.0 64 18459.0 65 13890.5 66 10716.5 67 8304.0 68 6301.0 69 4743.0 70 3630.0 71 2861.5 72 2451.0 73 2298.0 74 1928.0 75 1554.5 76 1138.0 77 724.0 78 527.0 79 372.0 80 249.5 81 188.0 82 137.0 83 92.5 84 56.5 85 28.0 86 15.0 87 11.5 88 7.5 89 6.0 90 4.5 91 2.5 92 1.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.037062587076789895 2 0.0059178400898159044 3 0.004193212406498127 4 5.748758944392593E-4 5 8.115894980318955E-4 6 2.0289737450797387E-4 7 0.0 8 0.0 9 0.0015893627669791286 10-11 4.5651909264294124E-4 12-13 0.0011328436743361875 14-15 0.001741535797860109 16-17 0.0026038496395189977 18-19 0.0 20-21 0.0 22-23 0.0015386384233521352 24-25 8.115894980318955E-4 26-27 0.004328477322836775 28-29 0.0052076992790379955 30-31 0.0011159355597938562 32-33 3.0434606176196077E-4 34-35 0.008403332927538585 36-37 0.010702836505295622 38-39 0.008284976125742267 40-41 0.0016400871106061223 42-43 3.2125417630429195E-4 44-45 0.0010144868725398693 46-47 5.072434362699347E-5 48-49 5.917840089815905E-4 50-51 7.946813834895643E-4 52-53 0.002519309066807342 54-55 0.0024685847231803487 56-57 0.0013864653924711549 58-59 8.792219562012201E-4 60-61 0.0031787255339582573 62-63 0.004362293551921438 64-65 0.008166619323945948 66-67 0.0030941849612466012 68-69 0.003060368732161939 70-71 0.0 72-73 0.0011328436743361875 74-75 0.0055458615698846195 76-77 0.0020120656305374074 78-79 0.00321254176304292 80-81 0.004497558468260087 82-83 0.004345385437379107 84-85 0.0050217100190723535 86-87 0.0033816229084662314 88-89 0.008065170636691961 90-91 0.011497517888785185 92-93 0.0011666599034208499 94-95 0.0022318711195877124 96-97 0.001200476132505512 98-99 0.0035337959393472113 100-101 0.004446834124633094 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2957160.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.148407170172106 #Duplication Level Percentage of deduplicated Percentage of total 1 71.9105037986198 35.34286720506773 2 15.094378526410864 14.83729323593487 3 5.412738970764988 7.980824965230476 4 2.6355886290987707 5.181399323040883 5 1.3604738200873263 3.343256062700569 6 0.8347129144514229 2.461488611379574 7 0.5564007444422929 1.914234723634568 8 0.3895118483945741 1.5315109538002298 9 0.269944645817528 1.1940614429442984 >10 1.3598215259824815 12.077503911991942 >50 0.1100096489716669 3.771702099301719 >100 0.060406291127912834 5.410825997002569 >500 0.0035117553419666656 1.130410010591251 >1k 0.001514874853295203 1.7846886984401202 >5k 4.131476872623281E-4 1.4668729865400318 >10k+ 6.885794787705467E-5 0.5710597723993285 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 16874 0.5706150495745919 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9118 0.308336376793951 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 8444 0.28554423839088855 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8153 0.27570371572725183 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6778 0.22920640073584114 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5734 0.19390225757145368 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5117 0.17303764422621706 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4683 0.1583614008034736 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4459 0.15078656548850924 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4274 0.1445305631078467 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4090 0.13830837695626885 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3586 0.12126499749759904 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3409 0.11527952494961383 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3091 0.1045259641006912 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.763245816932463E-5 2 0.0 0.0 0.0 0.0 6.763245816932463E-5 3 0.0 0.0 0.0 0.0 6.763245816932463E-5 4 0.0 0.0 0.0 0.0 6.763245816932463E-5 5 0.0 0.0 0.0 0.0 6.763245816932463E-5 6 0.0 0.0 0.0 0.0 6.763245816932463E-5 7 0.0 0.0 0.0 0.0 6.763245816932463E-5 8 0.0 0.0 0.0 0.0 6.763245816932463E-5 9 0.0 0.0 0.0 0.0 6.763245816932463E-5 10-11 0.0 0.0 0.0 0.0 6.763245816932463E-5 12-13 0.0 0.0 0.0 1.6908114542331157E-5 1.0144868725398694E-4 14-15 0.0 0.0 0.0 3.3816229084662314E-5 1.0144868725398694E-4 16-17 1.6908114542331157E-5 0.0 0.0 6.763245816932463E-5 1.0144868725398694E-4 18-19 3.3816229084662314E-5 0.0 0.0 6.763245816932463E-5 1.3526491633864926E-4 20-21 3.3816229084662314E-5 0.0 0.0 6.763245816932463E-5 1.8598925996564272E-4 22-23 3.3816229084662314E-5 0.0 0.0 1.0144868725398695E-4 2.0289737450797387E-4 24-25 6.763245816932463E-5 0.0 0.0 1.8598925996564272E-4 2.0289737450797387E-4 26-27 6.763245816932463E-5 0.0 0.0 3.21254176304292E-4 2.1980548905030503E-4 28-29 1.6908114542331157E-4 0.0 0.0 5.917840089815905E-4 2.3671360359263618E-4 30-31 2.0289737450797387E-4 0.0 0.0 0.0016908114542331157 2.3671360359263618E-4 32-33 2.0289737450797387E-4 0.0 0.0 0.003060368732161939 2.3671360359263618E-4 34-35 2.0289737450797387E-4 0.0 0.0 0.004413017895548431 2.3671360359263618E-4 36-37 2.3671360359263618E-4 0.0 0.0 0.006256002380662528 2.3671360359263618E-4 38-39 2.3671360359263618E-4 0.0 0.0 0.010127960610856362 2.3671360359263618E-4 40-41 2.3671360359263618E-4 0.0 0.0 0.013577215977491917 2.3671360359263618E-4 42-43 2.5362171813496733E-4 0.0 0.0 0.017094103802296798 2.3671360359263618E-4 44-45 3.043460617619608E-4 0.0 0.0 0.01885254771469924 2.3671360359263618E-4 46-47 3.5507040538895426E-4 0.0 0.0 0.02182837587414952 2.3671360359263618E-4 48-49 4.3961097810061005E-4 0.0 0.0 0.02630902622786728 2.3671360359263618E-4 50-51 4.3961097810061005E-4 0.0 0.0 0.03177034722504024 2.3671360359263618E-4 52-53 4.3961097810061005E-4 0.0 0.0 0.042270286355827885 2.3671360359263618E-4 54-55 4.3961097810061005E-4 0.0 0.0 0.05079197608516279 2.705298326772985E-4 56-57 4.5651909264294124E-4 0.0 0.0 0.0707097350160289 2.705298326772985E-4 58-59 4.7342720718527236E-4 0.0 0.0 0.103443844769982 2.705298326772985E-4 60-61 4.7342720718527236E-4 0.0 0.0 0.12782534594002354 2.705298326772985E-4 62-63 4.7342720718527236E-4 0.0 0.0 0.1416561836356504 2.705298326772985E-4 64-65 4.7342720718527236E-4 0.0 0.0 0.16581787931664166 2.705298326772985E-4 66-67 5.241515508122659E-4 0.0 0.0 0.22354218236416018 2.705298326772985E-4 68-69 5.41059665354597E-4 0.0 0.0 0.3039909913565718 2.705298326772985E-4 70-71 5.41059665354597E-4 0.0 0.0 0.37537705095429397 2.705298326772985E-4 72-73 5.41059665354597E-4 0.0 0.0 0.45631619526843326 2.705298326772985E-4 74-75 5.41059665354597E-4 0.0 0.0 0.5532165997105332 3.043460617619608E-4 76-77 5.41059665354597E-4 0.0 0.0 0.615962612777124 3.043460617619608E-4 78-79 5.579677798969282E-4 0.0 0.0 0.6998606771361712 3.043460617619608E-4 80-81 6.086921235239216E-4 0.0 0.0 0.8127561579353163 3.043460617619608E-4 82-83 6.086921235239216E-4 0.0 0.0 0.9101468976991438 3.043460617619608E-4 84-85 6.256002380662527E-4 0.0 0.0 1.045817608786809 3.043460617619608E-4 86-87 6.425083526085839E-4 0.0 0.0 1.2574057541695411 3.21254176304292E-4 88-89 6.425083526085839E-4 0.0 0.0 1.5342930379147561 3.3816229084662313E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12655 0.0 22.343021 1 GAGTACT 10795 0.0 21.53903 12-13 TACATGG 12855 0.0 21.431833 2 ACATGGG 12765 0.0 20.466234 3 GTATAGC 1080 0.0 20.240496 1 AGTACTT 11245 0.0 19.79002 12-13 GTACTTT 11765 0.0 19.601353 14-15 GTGTAGC 1940 0.0 17.881674 1 TAGCCCT 2040 0.0 17.694683 4 AGAGTAC 20090 0.0 17.520306 10-11 GTGTAGG 1425 0.0 17.007574 1 ACTTTTT 13840 0.0 16.850046 16-17 TACTTTT 13390 0.0 16.619492 14-15 GTATAAC 810 0.0 16.427069 1 CATGGGA 6765 0.0 16.35865 4 GGACCGT 385 4.66207E-9 16.037968 6 AGTACAT 10105 0.0 16.029556 2 TAAGGTG 1080 0.0 15.832354 5 ATAGCCC 1025 0.0 15.756187 3 CATGGGG 5955 0.0 15.473171 4 >>END_MODULE