FastQCFastQC Report
Thu 26 May 2016
SRR936432_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936432_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3066428
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT149080.4861682713567708No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT96940.3161332990697972No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75100.2449103647631707No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT74470.24285585704278723No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63000.2054507720383456No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC57880.1887538204060229No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT46490.1516096252708363No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT42340.138075963303231No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC38850.12669464275697978No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC35500.11576987948192491No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34920.11387842792982585No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC31770.10360588932790855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG130950.023.047081
GAGTACT89050.022.10143312-13
TACATGG136050.021.7632142
ACATGGG137650.020.6836243
GTGTAGC21600.020.2433221
GTACTTT97200.020.12321314-15
AGTACTT93250.019.70401412-13
AGAGTAC185000.017.6554210-11
CATGGGG63150.017.462724
TAGCCCT19750.017.0878944
GTGTAAG12550.017.041841
ACTTTTT119800.016.96004516-17
TAACACG3452.0803782E-816.5332974
CATGGGA72550.016.2484364
GTAGCCC18000.016.1076933
CGTACAC3251.900953E-716.0873813
TACTTTT114900.015.88564914-15
GTATCAA323650.015.1989091
AGTACAT104250.015.1810452
GTATAGC12100.014.5332931
Produced by FastQC (version 0.11.5)