##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936432_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3066428 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.145727210943807 31.0 31.0 34.0 28.0 34.0 2 31.18867816234394 31.0 31.0 34.0 28.0 34.0 3 31.139249641602543 31.0 31.0 34.0 27.0 34.0 4 34.78991288887266 37.0 35.0 37.0 32.0 37.0 5 34.83194159458497 37.0 35.0 37.0 32.0 37.0 6 34.88979196641826 37.0 35.0 37.0 32.0 37.0 7 34.74825725567337 37.0 35.0 37.0 32.0 37.0 8 34.73276985469739 37.0 35.0 37.0 32.0 37.0 9 36.18144466460651 39.0 35.0 39.0 32.0 39.0 10-11 36.04728139711743 39.0 35.0 39.0 30.0 39.0 12-13 35.94191450117205 38.5 35.0 39.0 30.0 39.0 14-15 36.872581550912 39.0 36.0 41.0 30.5 41.0 16-17 36.78552814545132 39.0 36.0 41.0 30.0 41.0 18-19 36.65260214816718 39.0 36.0 41.0 30.0 41.0 20-21 36.62983559372664 39.0 36.0 41.0 30.0 41.0 22-23 36.6303828102274 39.0 36.0 40.5 30.0 41.0 24-25 36.74762035828006 39.0 36.0 41.0 30.0 41.0 26-27 36.67770953695961 39.0 36.0 41.0 30.0 41.0 28-29 36.56465030321925 39.0 36.0 41.0 30.0 41.0 30-31 36.386497090425735 39.0 35.5 41.0 30.0 41.0 32-33 36.23098438965467 39.0 35.0 40.0 30.0 41.0 34-35 36.029169607112905 38.5 35.0 40.0 29.0 41.0 36-37 35.81692477371065 38.0 35.0 40.0 27.5 41.0 38-39 35.61846633933685 38.0 35.0 40.0 27.0 41.0 40-41 35.40933555263649 38.0 34.5 40.0 26.5 41.0 42-43 35.133101119608874 38.0 34.0 40.0 26.0 41.0 44-45 34.98947472433724 38.0 34.0 40.0 25.0 41.0 46-47 34.86381450991186 38.0 34.0 40.0 24.5 41.0 48-49 34.63311612077636 38.0 33.5 40.0 24.0 41.0 50-51 33.6547269330961 37.0 32.0 39.5 23.5 40.5 52-53 33.769972423940814 37.5 33.0 39.5 23.0 40.5 54-55 34.225173720041695 38.0 33.0 40.0 23.0 41.0 56-57 34.06654615728789 38.0 33.0 40.0 23.0 41.0 58-59 33.82792992367666 37.5 33.0 40.0 22.0 41.0 60-61 33.505545377227186 37.0 32.5 40.0 21.5 41.0 62-63 33.180266746846826 37.0 32.0 40.0 20.5 41.0 64-65 32.82106444371105 36.0 32.0 39.0 19.5 41.0 66-67 32.43811072687831 36.0 31.0 39.0 18.0 41.0 68-69 31.989559513544748 35.0 31.0 39.0 17.0 40.0 70-71 31.575495005915677 35.0 31.0 38.0 13.0 40.0 72-73 31.148105874326742 35.0 30.0 37.0 11.0 39.5 74-75 30.736105005563473 35.0 30.0 37.0 8.0 39.0 76-77 30.270191734487163 34.0 29.5 36.0 7.0 39.0 78-79 29.787425630081646 34.0 29.0 36.0 4.0 38.5 80-81 29.314037864251176 34.0 29.0 35.0 2.0 37.0 82-83 28.908508368694783 34.0 28.5 35.0 2.0 37.0 84-85 28.525277456375953 34.0 27.5 35.0 2.0 36.0 86-87 28.18441913522835 34.0 27.0 35.0 2.0 36.0 88-89 27.808821371315418 33.5 26.5 35.0 2.0 35.5 90-91 27.427267654743567 33.0 26.0 35.0 2.0 35.0 92-93 26.989064638074005 33.0 25.5 35.0 2.0 35.0 94-95 26.469701881146403 33.0 25.0 35.0 2.0 35.0 96-97 25.833707982055994 32.5 23.5 35.0 2.0 35.0 98-99 25.058445526847525 32.0 18.5 35.0 2.0 35.0 100-101 22.399464458320885 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.49630153638127084 1101 2 0.503118511604093 1101 3 0.6257394700346062 1101 4 0.537172883627342 1101 5 0.5102925845355912 1101 6 0.509088883763809 1101 7 0.47734669245935635 1101 8 0.542489311469744 1101 9 0.640650388706824 1101 10-11 0.7326010563153389 1101 12-13 0.7320797624810496 1101 14-15 1.0050357869744673 1101 16-17 1.027642600751257 1101 18-19 1.1741261833215972 1101 20-21 1.088151931656931 1101 22-23 0.8689517681136536 1101 24-25 0.8751008406688285 1101 26-27 0.8333722388057083 1101 28-29 0.8298557193669964 1101 30-31 0.7119566527344787 1101 32-33 0.5918563935141634 1101 34-35 0.8136540381824773 1101 36-37 0.7205719648291122 1101 38-39 0.6928326398613436 1101 40-41 0.6898625219703263 1101 42-43 0.7616586545387918 1101 44-45 0.7456302823786416 1101 46-47 0.8115433645502463 1101 48-49 0.8541575775999419 1101 50-51 0.7745683345330647 1101 52-53 0.7968122006126563 1101 54-55 0.9808317317069637 1101 56-57 1.0675617071778944 1101 58-59 0.8615310383114476 1101 60-61 0.9373716089028363 1101 62-63 1.0862490192621763 1101 64-65 1.036145398719185 1101 66-67 0.9854294432402995 1101 68-69 0.9843611822645606 1101 70-71 0.8623550927056165 1101 72-73 0.9417540022387989 1101 74-75 0.6052258474953618 1101 76-77 0.6353568270560324 1101 78-79 0.7068183785384967 1101 80-81 0.825504980922144 1101 82-83 0.8151539277603668 1101 84-85 0.8760242437288639 1101 86-87 0.863561077449635 1101 88-89 0.898716824966364 1101 90-91 0.8581602701177395 1101 92-93 0.9721559749855011 1101 94-95 1.1468438325172094 1101 96-97 1.1684717833586014 1101 98-99 1.2138879526872906 1101 100-101 1.5924129404748477 1102 1 0.3342801362011798 1102 2 0.479743432645936 1102 3 0.6138794720841574 1102 4 0.5107541357692895 1102 5 0.5185657612741821 1102 6 0.42267684734897415 1102 7 0.581843506413783 1102 8 0.43340452469036705 1102 9 0.5693235599015409 1102 10-11 0.6395173182514569 1102 12-13 0.5575979883752922 1102 14-15 0.8469990437127848 1102 16-17 0.5673681602621841 1102 18-19 0.6742312858663979 1102 20-21 0.7231845522949101 1102 22-23 0.4886016180931563 1102 24-25 0.6567625360516232 1102 26-27 0.694611890247451 1102 28-29 0.5613144649906943 1102 30-31 0.5686394106252521 1102 32-33 0.6572383136925097 1102 34-35 0.3369617087152932 1102 36-37 0.551338493123005 1102 38-39 0.380821140949962 1102 40-41 0.5875349573422355 1102 42-43 0.5021829408914655 1102 44-45 0.6786440521769777 1102 46-47 0.732576126724922 1102 48-49 0.9051863855669922 1102 50-51 0.7722919877085275 1102 52-53 0.5674550333960866 1102 54-55 0.8643326481255613 1102 56-57 0.7025472364159313 1102 58-59 0.6235047486055763 1102 60-61 0.710709886220684 1102 62-63 0.7114112929714622 1102 64-65 0.9032221788056063 1102 66-67 0.8518397513954952 1102 68-69 0.7601709968472896 1102 70-71 0.5984972014259604 1102 72-73 0.7759074364145704 1102 74-75 0.6332751022647685 1102 76-77 0.7294542639399069 1102 78-79 0.6878572389707145 1102 80-81 0.7743091218574101 1102 82-83 0.6651728588181385 1102 84-85 0.7275024831971919 1102 86-87 0.5714227793002813 1102 88-89 0.7987906592415932 1102 90-91 0.8759111029582876 1102 92-93 0.8263191592335168 1102 94-95 0.9995615680309093 1102 96-97 0.9218163227301623 1102 98-99 1.0154983420685966 1102 100-101 1.5247411812871405 1103 1 0.41383502283675 1103 2 0.4970064267307137 1103 3 0.5223389596987573 1103 4 0.5022899968128556 1103 5 0.4962351102146414 1103 6 0.4586813407055743 1103 7 0.5361112929845575 1103 8 0.3796349128689016 1103 9 0.5067332396646762 1103 10-11 0.43795187106503874 1103 12-13 0.4031295324924713 1103 14-15 0.7145138919186493 1103 16-17 0.904432908945374 1103 18-19 1.0054620589835679 1103 20-21 0.9117893462244311 1103 22-23 0.615245125621918 1103 24-25 0.4424083548161022 1103 26-27 0.599713890966008 1103 28-29 0.5038482894683014 1103 30-31 0.39105739463747113 1103 32-33 0.4967634618136856 1103 34-35 0.4738090973878357 1103 36-37 0.4592059370983961 1103 38-39 0.20004909639651913 1103 40-41 0.5911689183928672 1103 42-43 0.6793388177816695 1103 44-45 0.6094780979141063 1103 46-47 0.691862300130154 1103 48-49 0.6393429529408721 1103 50-51 0.5878855310045026 1103 52-53 0.6890351944608213 1103 54-55 0.6063937900139393 1103 56-57 0.745222646134998 1103 58-59 0.7554835657533019 1103 60-61 0.7391926886862095 1103 62-63 0.7251173638547002 1103 64-65 0.7929241280386137 1103 66-67 0.7389661704557327 1103 68-69 0.7485300615907668 1103 70-71 0.7106922779004314 1103 72-73 0.7948728817564934 1103 74-75 0.5938343298853965 1103 76-77 0.525798168917806 1103 78-79 0.60190871208275 1103 80-81 0.7076945889804414 1103 82-83 0.6967106104717722 1103 84-85 0.7507140823605916 1103 86-87 0.7110695439712522 1103 88-89 0.6884371733014767 1103 90-91 0.797720893952647 1103 92-93 0.7947672731143989 1103 94-95 0.9508781584048762 1103 96-97 1.0214715720902028 1103 98-99 1.3217471992820222 1103 100-101 1.830702838191339 1104 1 0.267513399018501 1104 2 0.20045180745927738 1104 3 0.4068035014520035 1104 4 0.4811327445298659 1104 5 0.4231669310103712 1104 6 0.3163039628293163 1104 7 0.4177833396641546 1104 8 0.4732424415644303 1104 9 0.5413603319274287 1104 10-11 0.5630310377636576 1104 12-13 0.5585841755330847 1104 14-15 0.47126951409605766 1104 16-17 0.5290600778828889 1104 18-19 0.5499825924866073 1104 20-21 0.5972839176508131 1104 22-23 0.4164155180433795 1104 24-25 0.49793507213923505 1104 26-27 0.1808052700383982 1104 28-29 0.16394870466216815 1104 30-31 0.15792512491096744 1104 32-33 0.1642792855690587 1104 34-35 0.2191558973663632 1104 36-37 0.18943736071882 1104 38-39 0.1591398007309479 1104 40-41 0.3292394745149636 1104 42-43 0.4500503500502404 1104 44-45 0.4560970760694687 1104 46-47 0.5093035097513052 1104 48-49 0.47510301075364936 1104 50-51 0.2890571693909578 1104 52-53 0.327878440563822 1104 54-55 0.4279572748055003 1104 56-57 0.5571818494074776 1104 58-59 0.4303458387001413 1104 60-61 0.3780062712380996 1104 62-63 0.35812374319545626 1104 64-65 0.36723256954726935 1104 66-67 0.3310279358156052 1104 68-69 0.258526055334098 1104 70-71 0.15659358022137582 1104 72-73 0.4774784916659378 1104 74-75 0.46703256866596377 1104 76-77 0.551540811973755 1104 78-79 0.3794769813798702 1104 80-81 0.5022412398265494 1104 82-83 0.3744195300476072 1104 84-85 0.32118404118624255 1104 86-87 0.3182192265377104 1104 88-89 0.29329974336304687 1104 90-91 0.4352666592051726 1104 92-93 0.33492496436044306 1104 94-95 0.6191779816695338 1104 96-97 0.626514711231902 1104 98-99 0.3358240638207093 1104 100-101 0.9251207233478702 1105 1 0.4579067300164432 1105 2 0.4470830053691017 1105 3 0.4939099515111174 1105 4 0.541996693384796 1105 5 0.5122092015946578 1105 6 0.492969023914398 1105 7 0.5807418426782718 1105 8 0.5098093797203518 1105 9 0.5863954357793659 1105 10-11 0.6938027386583983 1105 12-13 0.5764455408612719 1105 14-15 0.8723354013258486 1105 16-17 0.8029577001625228 1105 18-19 0.6728674212002446 1105 20-21 0.6819489714377909 1105 22-23 0.574931942413194 1105 24-25 0.49243449558948527 1105 26-27 0.5527352338398259 1105 28-29 0.6000718272264365 1105 30-31 0.3425638143185026 1105 32-33 0.5154142310522687 1105 34-35 0.28866845739322855 1105 36-37 0.4598152927297505 1105 38-39 0.11012568256014532 1105 40-41 0.43180840505166174 1105 42-43 0.23811130011666393 1105 44-45 0.34512465923128843 1105 46-47 0.5008705573786898 1105 48-49 0.7291675040466998 1105 50-51 0.7357472082168428 1105 52-53 0.7078418770922639 1105 54-55 0.590895434371113 1105 56-57 0.5489372791924794 1105 58-59 0.5725297580707576 1105 60-61 0.5628668228283544 1105 62-63 0.683150746158482 1105 64-65 0.8915141630140084 1105 66-67 0.7947649634462053 1105 68-69 0.8054190725138248 1105 70-71 0.8475653767212101 1105 72-73 0.6585345447085196 1105 74-75 0.5783247334278272 1105 76-77 0.540641923474098 1105 78-79 0.6208390436764368 1105 80-81 0.8835047413080623 1105 82-83 0.7878389079577239 1105 84-85 0.7343529118785384 1105 86-87 0.78914716146787 1105 88-89 0.7288452214614551 1105 90-91 0.779701585713326 1105 92-93 0.8081596456524984 1105 94-95 0.8615566690336074 1105 96-97 1.0241348926626195 1105 98-99 1.195078690814917 1105 100-101 1.4114283965682297 1106 1 0.21055230587419516 1106 2 0.12939779266890383 1106 3 0.1498864837634173 1106 4 0.22391714528617257 1106 5 0.26297409404757843 1106 6 0.05428222258796467 1106 7 0.3647281714291495 1106 8 0.37727739896728707 1106 9 0.40223811789774544 1106 10-11 0.4458837691643609 1106 12-13 0.5046055215591707 1106 14-15 0.3801778663492925 1106 16-17 0.2374210914541166 1106 18-19 0.39294320381226555 1106 20-21 0.17484659057625151 1106 22-23 -0.01914812764470497 1106 24-25 -0.21896617693910514 1106 26-27 -0.311613523443917 1106 28-29 -0.08755300907915142 1106 30-31 -0.11367207652572375 1106 32-33 -0.016837666781988503 1106 34-35 0.02896254938379883 1106 36-37 -0.12271186070604045 1106 38-39 -0.0669979026731724 1106 40-41 -0.05133082355557406 1106 42-43 0.09469837605385578 1106 44-45 -0.237239180418662 1106 46-47 -0.05978271388554646 1106 48-49 -0.033972426715777715 1106 50-51 -0.315023004136755 1106 52-53 -0.07052407511352499 1106 54-55 0.09278574554231511 1106 56-57 0.18037388881440108 1106 58-59 -0.03450485227159561 1106 60-61 0.1399644533947466 1106 62-63 0.07625746247038734 1106 64-65 -0.22650219111297076 1106 66-67 -0.19873011457094236 1106 68-69 -0.05156941411056337 1106 70-71 -0.10711009948602879 1106 72-73 0.021691877098955814 1106 74-75 0.15100415961653368 1106 76-77 0.007812986581914316 1106 78-79 0.028474059441794708 1106 80-81 0.10475130509107444 1106 82-83 0.10516704247633513 1106 84-85 0.3911188314126228 1106 86-87 0.5151146445153891 1106 88-89 0.4784803067258494 1106 90-91 0.5798206028177937 1106 92-93 0.6536486833433699 1106 94-95 0.7096005128964933 1106 96-97 0.6174708931111432 1106 98-99 0.8404603363694605 1106 100-101 1.4035806922486636 1107 1 0.2646358199603469 1107 2 0.23013508039974084 1107 3 0.24700784832605294 1107 4 0.30194992012125965 1107 5 0.3124087002742826 1107 6 0.2674952210494581 1107 7 0.40810529370906323 1107 8 0.47784227698502946 1107 9 0.5458294044200613 1107 10-11 0.5472628475013792 1107 12-13 0.3805124386906016 1107 14-15 0.14430586283487656 1107 16-17 0.32723133935223814 1107 18-19 0.5650411665084931 1107 20-21 0.3132623351456729 1107 22-23 0.3915227507078072 1107 24-25 0.34318769799303084 1107 26-27 0.35409665504824517 1107 28-29 0.2664078158627561 1107 30-31 0.15418743304881843 1107 32-33 0.37109003867876567 1107 34-35 0.2670048869335844 1107 36-37 0.29047772505383307 1107 38-39 0.38929125086621497 1107 40-41 0.351781202104263 1107 42-43 0.24095702893675508 1107 44-45 0.22297357264720574 1107 46-47 0.41509769367055327 1107 48-49 0.5023231551314637 1107 50-51 0.4806600438797446 1107 52-53 0.5085345331803524 1107 54-55 0.5255830138235282 1107 56-57 0.660464034216723 1107 58-59 0.6574971140139425 1107 60-61 0.65083861744818 1107 62-63 0.7087751739061119 1107 64-65 0.6491113087842635 1107 66-67 0.6071435263590033 1107 68-69 0.5610047235829931 1107 70-71 0.5373583182010755 1107 72-73 0.5754879549816856 1107 74-75 0.6048993405786867 1107 76-77 0.5647207173935733 1107 78-79 0.6730101321218918 1107 80-81 0.6354568335485133 1107 82-83 0.6430500245628004 1107 84-85 0.6556393222073389 1107 86-87 0.7915314488541156 1107 88-89 0.7264064709876656 1107 90-91 0.6472261694931554 1107 92-93 0.8545091629717625 1107 94-95 0.7848385860926932 1107 96-97 0.9704861517608201 1107 98-99 0.9081025438274892 1107 100-101 1.4708923394046032 1108 1 0.08136861633007442 1108 2 0.06104780965990031 1108 3 0.1906014108596672 1108 4 0.27644358260065616 1108 5 0.3164779524664496 1108 6 0.1518880449859239 1108 7 0.3834701175845652 1108 8 0.3138939178160385 1108 9 -0.13829123601682625 1108 10-11 -0.005912662951551795 1108 12-13 -0.030410873064020905 1108 14-15 0.42419359258496314 1108 16-17 0.5124967182643303 1108 18-19 0.4599784663175228 1108 20-21 0.41190568548503137 1108 22-23 0.49659638178810894 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10-11 0.27438532743342847 2311 12-13 0.4472054549011375 2311 14-15 0.6300921453106838 2311 16-17 0.7038391812665807 2311 18-19 0.8060216209486839 2311 20-21 0.8108364849377523 2311 22-23 0.6825895784692904 2311 24-25 0.6121314970751044 2311 26-27 0.5342538530425855 2311 28-29 0.724599840701913 2311 30-31 0.7123178628074598 2311 32-33 0.5838629921532075 2311 34-35 0.6439034440905829 2311 36-37 0.6556559567528666 2311 38-39 0.6867017433996168 2311 40-41 0.8094870415983095 2311 42-43 0.8316087909476764 2311 44-45 0.8869867193774184 2311 46-47 0.7746685490444349 2311 48-49 0.7911953801498015 2311 50-51 0.8475074829216354 2311 52-53 0.7774030547582171 2311 54-55 0.7114259115578037 2311 56-57 0.6158856341707022 2311 58-59 0.7234099903349858 2311 60-61 0.6721705796382764 2311 62-63 0.7323093777896972 2311 64-65 0.6829846868973206 2311 66-67 0.6881979142349266 2311 68-69 0.6820831016307771 2311 70-71 0.718498330007602 2311 72-73 0.7548953754967123 2311 74-75 0.6645421374594811 2311 76-77 0.80676114871385 2311 78-79 0.8147555452464808 2311 80-81 0.7903878968565579 2311 82-83 0.6851925664090217 2311 84-85 0.9662271007570702 2311 86-87 1.1468113414446712 2311 88-89 0.9554544015331707 2311 90-91 0.8359886450352185 2311 92-93 0.9411378089388052 2311 94-95 0.8317153915803637 2311 96-97 0.8327977948265115 2311 98-99 1.0906937144579487 2311 100-101 0.7394566083594718 2312 1 0.263357472221454 2312 2 0.2778964702810711 2312 3 0.32281503607197237 2312 4 0.2953045394842775 2312 5 0.3226216141305187 2312 6 0.2591785963312674 2312 7 0.4036195996119645 2312 8 0.47253884585608574 2312 9 0.536972021201521 2312 10-11 0.3819318658075943 2312 12-13 0.184273675162558 2312 14-15 0.4034867064793488 2312 16-17 0.29214123907759415 2312 18-19 0.30704231153161743 2312 20-21 0.36575904411277804 2312 22-23 0.290435437816555 2312 24-25 0.30057546908481214 2312 26-27 0.3013124469562669 2312 28-29 0.242298537120206 2312 30-31 0.4490000918858712 2312 32-33 0.4266997434097064 2312 34-35 0.3918006439591437 2312 36-37 0.5552277882730365 2312 38-39 0.5185642376932833 2312 40-41 0.6004665583181605 2312 42-43 0.3082005014824105 2312 44-45 0.2565306311811071 2312 46-47 0.6672279225489319 2312 48-49 0.3851253228327849 2312 50-51 0.3461468929467131 2312 52-53 0.21511303153589267 2312 54-55 0.13666039527461038 2312 56-57 0.4851096049895247 2312 58-59 0.518954193244717 2312 60-61 0.34081255889476836 2312 62-63 0.35456267092687455 2312 64-65 0.42358526955048603 2312 66-67 0.5672697600898644 2312 68-69 0.4073544698071494 2312 70-71 0.35971183424077324 2312 72-73 0.24982097805315462 2312 74-75 0.08215443933645616 2312 76-77 0.12172377907753784 2312 78-79 0.22612191323539932 2312 80-81 0.20302840514536058 2312 82-83 0.1422627494309232 2312 84-85 -0.22484448927740175 2312 86-87 -0.15929692354122338 2312 88-89 0.004498272537905024 2312 90-91 0.126979574435385 2312 92-93 0.18235605467200955 2312 94-95 0.09182333237997753 2312 96-97 -0.02709650280295861 2312 98-99 0.16803259718411567 2312 100-101 -0.018664919594424845 2313 1 0.007353095719302871 2313 2 -0.005034998146243197 2313 3 0.03331340224422519 2313 4 0.17988685153996187 2313 5 0.07822105161056925 2313 6 0.09116386482066474 2313 7 0.2014604227476724 2313 8 0.17755760012899913 2313 9 0.28175652349996483 2313 10-11 0.3037641840331702 2313 12-13 0.21604114685796105 2313 14-15 0.1311645105386532 2313 16-17 0.2738778505443946 2313 18-19 0.029307344067461827 2313 20-21 0.05243246950506375 2313 22-23 0.010179208077040869 2313 24-25 0.05527053192450637 2313 26-27 -0.1152938198241813 2313 28-29 -0.006182589755489687 2313 30-31 0.11585899905059449 2313 32-33 0.026625347635878427 2313 34-35 0.008388336150929376 2313 36-37 0.12993757656169436 2313 38-39 0.08372765077611888 2313 40-41 0.3739797036281658 2313 42-43 0.4709888568173213 2313 44-45 0.5628636999672665 2313 46-47 0.3578058000839448 2313 48-49 0.36640184520216934 2313 50-51 0.480790037862711 2313 52-53 0.4026933752913422 2313 54-55 0.17949560490118444 2313 56-57 0.43036841958439 2313 58-59 0.44474665490594845 2313 60-61 0.37463557510488954 2313 62-63 0.2141359662970359 2313 64-65 0.22266526454531288 2313 66-67 0.27371835718884796 2313 68-69 0.03656514287836998 2313 70-71 0.021084580772974704 2313 72-73 0.23369561559971785 2313 74-75 0.2265355504951927 2313 76-77 0.0879017911808937 2313 78-79 -0.0828977598085423 2313 80-81 0.06123923928294417 2313 82-83 -0.1155535695358978 2313 84-85 -0.08122010859272066 2313 86-87 0.07121599489035191 2313 88-89 0.1677074245711303 2313 90-91 0.06026930727737678 2313 92-93 0.2847747237356195 2313 94-95 0.25438340212344457 2313 96-97 0.1305436281803658 2313 98-99 0.21279178132236964 2313 100-101 -0.0521184465364577 2314 1 -0.5338701267311343 2314 2 -0.4958620740963404 2314 3 -0.49492824526772594 2314 4 -0.22466929652770062 2314 5 -0.1784338018790308 2314 6 -0.20693178325996797 2314 7 -0.4135437417811332 2314 8 -0.22717571911960022 2314 9 -0.43447401930784935 2314 10-11 -0.4179670315062154 2314 12-13 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-0.780141632172981 2314 78-79 -0.7169893392001363 2314 80-81 -0.6256590419024413 2314 82-83 -0.570078874144837 2314 84-85 -0.749986513209123 2314 86-87 -0.7683752511800854 2314 88-89 -0.5833962946999165 2314 90-91 -0.6770621855089622 2314 92-93 -0.73888975450161 2314 94-95 -0.8152843198395878 2314 96-97 -0.8723472908170358 2314 98-99 -0.6952737434214953 2314 100-101 -1.0685837173314319 2315 1 -0.7836451375668645 2315 2 -1.265157948638283 2315 3 -1.1311639782227942 2315 4 -0.8735827026246028 2315 5 -0.8615891665506084 2315 6 -0.5227204274145905 2315 7 -0.5822979611488535 2315 8 -0.34832259908597507 2315 9 -0.750834790023795 2315 10-11 -0.8250177427241496 2315 12-13 -0.8073507838165099 2315 14-15 -0.7155191037901218 2315 16-17 -1.152728540003281 2315 18-19 -1.1092653749930648 2315 20-21 -1.299240472593091 2315 22-23 -1.1406271648834405 2315 24-25 -1.364097448185973 2315 26-27 -1.2006683071973825 2315 28-29 -1.2570573928988011 2315 30-31 -1.1035687775280607 2315 32-33 -1.221889413617923 2315 34-35 -0.9612099562921657 2315 36-37 -1.0361520701754827 2315 38-39 -1.2921711748788098 2315 40-41 -1.3992158100378873 2315 42-43 -1.5243500564538195 2315 44-45 -1.5338724361256624 2315 46-47 -1.9731355146389404 2315 48-49 -2.5533319485959396 2315 50-51 -2.1676399850805907 2315 52-53 -2.0172028602491814 2315 54-55 -2.194227684544096 2315 56-57 -2.3864665245762637 2315 58-59 -2.585162291381156 2315 60-61 -2.6339754360789733 2315 62-63 -2.772892516967147 2315 64-65 -2.2182857231356614 2315 66-67 -2.1045208446721944 2315 68-69 -2.2659658145845967 2315 70-71 -2.052562923843663 2315 72-73 -2.007836548984468 2315 74-75 -2.0283536101958255 2315 76-77 -1.8956034969693327 2315 78-79 -1.884333994229884 2315 80-81 -2.205655101636868 2315 82-83 -2.230011364261337 2315 84-85 -2.076194007594246 2315 86-87 -2.065774675901313 2315 88-89 -2.145714484279228 2315 90-91 -2.019885779819216 2315 92-93 -1.9734795071842726 2315 94-95 -2.2278850264605445 2315 96-97 -2.1330686221014297 2315 98-99 -2.191828375203677 2315 100-101 -2.381098788847254 2316 1 -5.289259153969038 2316 2 -5.34020214929863 2316 3 -6.055998713906394 2316 4 -8.059909965023866 2316 5 -8.761273193958566 2316 6 -9.757993974473202 2316 7 -11.66263346013703 2316 8 -11.550187902455164 2316 9 -11.936581940945082 2316 10-11 -11.524567925562224 2316 12-13 -10.12366278012425 2316 14-15 -10.058961784941822 2316 16-17 -9.057645996601433 2316 18-19 -9.428379054121123 2316 20-21 -10.332446351813456 2316 22-23 -9.903226483234707 2316 24-25 -9.88949791681948 2316 26-27 -8.079282110584323 2316 28-29 -6.436179770148776 2316 30-31 -8.638790668393256 2316 32-33 -6.340893212389542 2316 34-35 -4.63523931689145 2316 36-37 -5.194148484419102 2316 38-39 -4.247887261078972 2316 40-41 -3.699519356741476 2316 42-43 -3.949185679700882 2316 44-45 -4.306799442906641 2316 46-47 -4.151309974112792 2316 48-49 -5.004967195516095 2316 50-51 -4.98431392976763 2316 52-53 -4.8353764321595705 2316 54-55 -4.12146824974451 2316 56-57 -4.658650285715186 2316 58-59 -5.091030683471189 2316 60-61 -5.382580363831309 2316 62-63 -5.024452498505827 2316 64-65 -5.827491531350947 2316 66-67 -5.412706309932965 2316 68-69 -5.6017603613946925 2316 70-71 -5.126610390737547 2316 72-73 -4.259283277425549 2316 74-75 -4.524751700159257 2316 76-77 -5.11321470867097 2316 78-79 -5.140368715712469 2316 80-81 -5.778339093542289 2316 82-83 -5.193850076229243 2316 84-85 -5.080627919618955 2316 86-87 -5.879640192285731 2316 88-89 -7.220096917623213 2316 90-91 -6.536020440947485 2316 92-93 -5.799863751157712 2316 94-95 -5.185822328977675 2316 96-97 -6.7395721546040015 2316 98-99 -8.122342629472861 2316 100-101 -8.549522476140895 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8244.0 3 3199.0 4 3379.0 5 3741.0 6 3948.0 7 4850.0 8 6147.0 9 7667.0 10 9495.0 11 11882.0 12 14382.0 13 16734.0 14 18748.0 15 22062.0 16 25079.0 17 29494.0 18 25395.0 19 21773.0 20 22611.0 21 24762.0 22 27684.0 23 31491.0 24 36149.0 25 41630.0 26 47911.0 27 56596.0 28 66305.0 29 78561.0 30 95332.0 31 114563.0 32 140295.0 33 175847.0 34 225362.0 35 297965.0 36 402192.0 37 508495.0 38 383818.0 39 52625.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.69439240745938 18.47910941691742 14.222306351703295 22.604191823919898 2 15.404020221481133 20.1739188302675 39.793498709652376 24.628562238598995 3 20.146165432367745 24.1596971667768 29.466356249131596 26.227781151723857 4 12.750635814431236 16.436320864727197 37.79949004371846 33.01355327712311 5 13.853598706947873 37.483115133505024 33.97690186145358 14.68638429809353 6 31.65889946481011 35.93970093826233 17.58496457620959 14.816435020717975 7 28.634967382974917 32.59571337748414 20.539359095365473 18.229960144175475 8 26.119420517602897 33.8267204321776 19.890324100570826 20.163534949648675 9 26.117173584282522 17.385482568730694 20.788484847298125 35.708858999688665 10-11 25.165320013535602 26.18918646400682 27.10946379373414 21.53602972872344 12-13 26.605673099886072 23.721686374355166 27.35440105026472 22.31823947549404 14-15 23.13434138490148 25.22164339040608 26.339775055511787 25.30424016918065 16-17 22.240196596692236 28.410649792400054 26.77112579081809 22.57802782008962 18-19 22.059991741277152 27.4785264232633 28.739392916978623 21.72208891848092 20-21 23.308709787635166 26.10330146868754 28.372978628154016 22.21501011552327 22-23 22.578549325462024 26.107999461837338 28.605074221600706 22.708376991099932 24-25 22.97974361483511 26.233959617705583 28.556496069413186 22.229800698046123 26-27 22.534699920832217 26.20016498996458 29.026115582352922 22.23901950685028 28-29 22.04577597367178 27.15852815955043 28.65706927644192 22.13862659033587 30-31 22.281348981496958 26.329239210634665 28.566855950201987 22.82255585766639 32-33 22.282231763520045 26.512950629409836 28.54875425073624 22.65606335633388 34-35 23.18995384122392 26.42620507347 28.160114936249357 22.223726149056724 36-37 22.778730524252257 26.09594063689195 28.694951849658946 22.430376989196848 38-39 22.577959201245353 26.579739299434497 28.632037918896273 22.210263580423877 40-41 22.726578221689472 26.629538017702586 28.358019362578478 22.285864398029467 42-43 22.410102313925982 26.722252895709453 27.84513624844155 23.022508541923013 44-45 22.730089904645993 26.48437564967957 27.788895536172447 22.99663890950199 46-47 23.11667143881143 26.902226906678372 27.584654478239344 22.396447176270854 48-49 23.09286261344957 26.851526490956562 27.47700027605702 22.578610619536846 50-51 22.36963347114412 27.124912662313573 27.936922827319638 22.568531039222673 52-53 22.997972392910633 27.351857090795544 27.003258683368 22.646911832925827 54-55 22.517581172439062 26.839592560639534 27.426677921921645 23.216148344999763 56-57 23.03796217512439 26.95995061130898 27.096907695262907 22.905179518303722 58-59 23.51338843988622 26.67349791377366 27.05382574840965 22.759287897930474 60-61 22.994350254847586 26.80523356829503 27.892801764503776 22.30761441235361 62-63 22.646174216266502 27.008665889951594 27.655709222662576 22.689450671119328 64-65 22.48305956826011 27.391916238111286 27.24116975358616 22.88385444004244 66-67 22.73114904110965 27.09229264589296 26.882242388409374 23.29431592458801 68-69 22.560911678087155 27.010622253754512 27.318806703288978 23.109659364869355 70-71 22.990254787611768 26.877594164449746 27.1033002296684 23.02885081827009 72-73 22.87029537910143 26.586483936109108 27.700308142536876 22.84291254225258 74-75 22.487832203869395 27.655832999699122 27.056172042730186 22.8001627537013 76-77 22.818138909357316 26.940676133355645 27.020739492519972 23.220445464767067 78-79 22.829193940926956 27.067743236497172 27.11720752020199 22.985855302373878 80-81 23.004024630477605 27.119812662257186 26.972819067746862 22.903343639518347 82-83 22.86728544033813 27.034647846902438 27.27976570958947 22.818301003169967 84-85 22.670809529671992 27.27607300939971 27.097014578562067 22.956102882366224 86-87 22.717217894264486 27.314056890758053 27.034507925989317 22.934217288988144 88-89 22.608686719330645 27.263947241663484 26.608691285006092 23.518674753999775 90-91 22.72455392522968 26.878846385905636 27.02206973475225 23.374529954112433 92-93 23.097427925976167 27.093704588599106 27.109457671580184 22.699409813844547 94-95 22.680103862826964 26.949442052050788 27.156913300110535 23.21354078501171 96-97 22.246364923488983 26.971858387510363 27.669699145050487 23.11207754395017 98-99 22.403468321410788 27.240225326072544 26.812230393120245 23.544075959396423 100-101 22.344189423596927 26.88107140503998 27.081752726689164 23.69298644467393 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 204.0 1 170.5 2 178.0 3 207.5 4 198.0 5 230.5 6 389.0 7 747.5 8 1223.5 9 1580.0 10 1631.5 11 1593.5 12 1661.5 13 1838.0 14 2099.5 15 2324.5 16 2511.5 17 2764.5 18 3064.0 19 3361.0 20 3818.0 21 4496.5 22 5332.0 23 6666.0 24 8186.5 25 10269.5 26 13618.5 27 17820.0 28 21847.0 29 26419.0 30 32379.0 31 38989.5 32 45419.0 33 50729.0 34 56534.5 35 62566.5 36 69184.5 37 75900.5 38 80174.0 39 82887.5 40 86363.5 41 91458.0 42 96394.0 43 101535.0 44 106371.5 45 110916.5 46 114674.0 47 126601.0 48 154238.0 49 153562.0 50 126806.0 51 113430.0 52 106983.0 53 104445.5 54 100731.0 55 92722.0 56 84691.5 57 76508.0 58 70002.0 59 62570.5 60 52647.5 61 43122.0 62 34483.0 63 26863.0 64 20024.0 65 15137.0 66 11561.0 67 9045.5 68 6980.0 69 5139.0 70 3911.0 71 3145.0 72 2659.5 73 2535.5 74 2073.0 75 1533.0 76 1139.5 77 744.5 78 489.0 79 339.5 80 232.5 81 155.0 82 103.5 83 74.0 84 52.5 85 30.5 86 20.0 87 14.5 88 14.0 89 12.5 90 7.0 91 4.5 92 3.5 93 5.0 94 4.5 95 2.0 96 1.5 97 1.0 98 1.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07722340129949244 2 0.031795952815458245 3 0.014675055145596113 4 0.02201258271839417 5 0.026512932963043648 6 0.031763341581801365 7 0.07774518103800251 8 0.05746099370342301 9 0.07246216118558792 10-11 0.06251573492023944 12-13 0.042264158819316805 14-15 0.05017238298111027 16-17 0.061309119274934876 18-19 0.05835780262898722 20-21 0.019419989642672192 22-23 0.014365248425855752 24-25 0.023740978102208824 26-27 0.025665040887964757 28-29 0.006929887152087054 30-31 0.0013207549631036502 32-33 0.011136736293824607 34-35 0.012408574406442937 36-37 0.004777545730732957 38-39 0.009946426265348477 40-41 0.0049732131326742385 42-43 0.0026415099262073003 44-45 4.0764042071100317E-4 46-47 7.174471404513656E-4 48-49 0.0010598650938486083 50-51 8.315864582504464E-4 52-53 1.793617851128414E-4 54-55 2.771954860834821E-4 56-57 0.004532961478306356 58-59 0.004109015440766912 60-61 0.005984161376037527 62-63 0.003114372814232064 64-65 7.011415236229255E-4 66-67 6.196134394807248E-4 68-69 0.002690426776692621 70-71 0.0016468672996724526 72-73 0.020838578306746482 74-75 0.014528304594140153 76-77 0.023838811803179465 78-79 0.018898209904162105 80-81 0.010044259966319118 82-83 0.004826462581218278 84-85 0.003603541319085268 86-87 0.001956674019412815 88-89 0.0022501751223247377 90-91 0.0077614736103375 92-93 0.011788960966962211 94-95 0.015033778715821795 96-97 0.013011882229095221 98-99 0.024050784821949188 100-101 0.020284187334579516 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3066428.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.4372860640916 #Duplication Level Percentage of deduplicated Percentage of total 1 73.71867678192574 37.91888665898222 2 14.185460796684932 14.593232099000792 3 5.171803849639285 7.980706622437982 4 2.356330240173302 4.848129305010552 5 1.2796587767344063 3.2911087281656592 6 0.7619058800395719 2.351422242330535 7 0.505615203189691 1.8205231691375354 8 0.3397669135894848 1.3981350343532657 9 0.26565372575164914 1.2298056016931382 >10 1.2581620451461752 11.691249484609502 >50 0.09942169887468633 3.5631445656213123 >100 0.052752955506931515 4.929240757003025 >500 0.003002443186589446 0.9909631918702388 >1k 0.0014053989380801402 1.696381249880442 >5k 3.194088495636683E-4 1.2072085913949862 >10k+ 6.388176991273366E-5 0.4898626985088449 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 14908 0.4861682713567708 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 9694 0.3161332990697972 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7510 0.2449103647631707 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7447 0.24285585704278723 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6300 0.2054507720383456 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5788 0.1887538204060229 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4649 0.1516096252708363 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4234 0.138075963303231 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3885 0.12669464275697978 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3550 0.11576987948192491 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3492 0.11387842792982585 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3177 0.10360588932790855 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.261123365688025E-5 0.0 5 0.0 0.0 0.0 3.261123365688025E-5 0.0 6 0.0 0.0 0.0 3.261123365688025E-5 0.0 7 0.0 0.0 0.0 3.261123365688025E-5 0.0 8 0.0 0.0 0.0 3.261123365688025E-5 0.0 9 0.0 0.0 0.0 3.261123365688025E-5 0.0 10-11 0.0 0.0 0.0 3.261123365688025E-5 0.0 12-13 0.0 0.0 0.0 3.261123365688025E-5 0.0 14-15 0.0 0.0 0.0 3.261123365688025E-5 0.0 16-17 0.0 0.0 0.0 3.261123365688025E-5 0.0 18-19 0.0 0.0 0.0 3.261123365688025E-5 0.0 20-21 0.0 0.0 0.0 3.261123365688025E-5 1.6305616828440126E-5 22-23 0.0 0.0 0.0 4.891685048532038E-5 3.261123365688025E-5 24-25 0.0 0.0 0.0 9.783370097064075E-5 3.261123365688025E-5 26-27 0.0 0.0 0.0 2.1197301876972163E-4 3.261123365688025E-5 28-29 4.891685048532038E-5 0.0 0.0 5.706965889954044E-4 3.261123365688025E-5 30-31 6.52224673137605E-5 0.0 0.0 0.0014022830472458507 3.261123365688025E-5 32-33 6.52224673137605E-5 3.261123365688025E-5 0.0 0.0025436762252366595 4.891685048532038E-5 34-35 8.152808414220062E-5 3.261123365688025E-5 0.0 0.0033752626834871063 6.52224673137605E-5 36-37 9.783370097064075E-5 3.261123365688025E-5 0.0 0.00526671423558616 6.52224673137605E-5 38-39 9.783370097064075E-5 3.261123365688025E-5 0.0 0.008071280330077863 8.152808414220062E-5 40-41 1.1413931779908089E-4 3.261123365688025E-5 0.0 0.011479154247221849 9.783370097064075E-5 42-43 1.4675055145596115E-4 3.261123365688025E-5 0.0 0.015245751734591518 9.783370097064075E-5 44-45 1.956674019412815E-4 3.261123365688025E-5 0.0 0.017332870688631853 1.1413931779908089E-4 46-47 2.2827863559816177E-4 3.261123365688025E-5 0.0 0.020300492951407956 1.4675055145596115E-4 48-49 2.9350110291192225E-4 3.261123365688025E-5 0.0 0.02445842524266019 1.9566740194128154E-4 50-51 2.9350110291192225E-4 3.261123365688025E-5 0.0 0.02990450126335919 2.2827863559816177E-4 52-53 2.9350110291192225E-4 3.261123365688025E-5 0.0 0.04074773645427188 2.60889869255042E-4 54-55 2.9350110291192225E-4 3.261123365688025E-5 0.0 0.048525515681437814 3.424179533972427E-4 56-57 2.9350110291192225E-4 3.261123365688025E-5 0.0 0.06768461545485496 3.587235702256828E-4 58-59 2.9350110291192225E-4 3.261123365688025E-5 0.0 0.09928490086837194 3.587235702256828E-4 60-61 2.9350110291192225E-4 3.261123365688025E-5 0.0 0.12581413944824402 3.587235702256828E-4 62-63 2.9350110291192225E-4 3.261123365688025E-5 0.0 0.13942932949999154 3.587235702256828E-4 64-65 2.9350110291192225E-4 3.261123365688025E-5 0.0 0.16641512535105993 3.587235702256828E-4 66-67 2.9350110291192225E-4 3.261123365688025E-5 0.0 0.2247566223632187 3.587235702256828E-4 68-69 3.91334803882563E-4 3.261123365688025E-5 0.0 0.3056650930659386 3.587235702256828E-4 70-71 3.91334803882563E-4 3.261123365688025E-5 0.0 0.37489874211949537 3.7502918705412287E-4 72-73 4.0764042071100317E-4 3.261123365688025E-5 0.0 0.4526765343911548 4.0764042071100317E-4 74-75 4.239460375394433E-4 3.261123365688025E-5 0.0 0.5463196918368864 4.4025165436788346E-4 76-77 4.239460375394433E-4 3.261123365688025E-5 0.0 0.6095528738975773 4.891685048532038E-4 78-79 4.5655727119632355E-4 3.261123365688025E-5 0.0 0.6928908815077347 4.891685048532038E-4 80-81 4.891685048532038E-4 3.261123365688025E-5 0.0 0.8060355566802808 4.891685048532038E-4 82-83 5.21779738510084E-4 3.261123365688025E-5 0.0 0.9041953699874903 4.891685048532038E-4 84-85 5.380853553385242E-4 3.261123365688025E-5 0.0 1.04082013339299 4.891685048532038E-4 86-87 5.543909721669643E-4 3.261123365688025E-5 0.0 1.253510599303163 5.054741216816439E-4 88-89 5.543909721669643E-4 3.261123365688025E-5 0.0 1.5252600093659463 5.21779738510084E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 13095 0.0 23.04708 1 GAGTACT 8905 0.0 22.101433 12-13 TACATGG 13605 0.0 21.763214 2 ACATGGG 13765 0.0 20.683624 3 GTGTAGC 2160 0.0 20.243322 1 GTACTTT 9720 0.0 20.123213 14-15 AGTACTT 9325 0.0 19.704014 12-13 AGAGTAC 18500 0.0 17.65542 10-11 CATGGGG 6315 0.0 17.46272 4 TAGCCCT 1975 0.0 17.087894 4 GTGTAAG 1255 0.0 17.04184 1 ACTTTTT 11980 0.0 16.960045 16-17 TAACACG 345 2.0803782E-8 16.533297 4 CATGGGA 7255 0.0 16.248436 4 GTAGCCC 1800 0.0 16.107693 3 CGTACAC 325 1.900953E-7 16.087381 3 TACTTTT 11490 0.0 15.885649 14-15 GTATCAA 32365 0.0 15.198909 1 AGTACAT 10425 0.0 15.181045 2 GTATAGC 1210 0.0 14.533293 1 >>END_MODULE