FastQCFastQC Report
Thu 26 May 2016
SRR936432_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936432_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3066428
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT170560.5562172012517496No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93530.305012868392801No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT86530.2821850048329848No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82920.2704123494828511No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT68450.22322389438134532No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC58890.1920475550053678No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT51120.16670862645397186No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48210.1572187574598197No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC46770.15252273981322895No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC44040.14361987302490062No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT42590.13889124414465298No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG35900.11707432882820011No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA35200.1147915424722185No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC32690.10660612282434154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG135700.023.916731
TACATGG137750.022.969872
ACATGGG136950.022.0232473
GAGTACT108950.021.99253312-13
GTACTTT119950.019.77741414-15
AGTACTT115250.019.76009412-13
AGAGTAC200850.018.49107210-11
TAGCGCG3156.830305E-918.092474
CATGGGG65400.017.7916224
TTTACGC1350.00940685617.5916233
ACTTTTT139200.017.16180816-17
CATGGGA73750.017.0651864
GTGTAGC18550.016.9067821
TATTCGC1750.002168101316.2888012
TACTTTT138200.016.23783114-15
TACACCG3854.667527E-916.0365095
AGTACAT105400.015.4156352
GAGTACA124550.015.0318851
GTATCAA348650.014.67867851
TACCTGG21050.014.4445432
Produced by FastQC (version 0.11.5)