FastQCFastQC Report
Thu 26 May 2016
SRR936431_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936431_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1384439
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42940.31016173338081343No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36150.26111659668645565No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT24740.17870054224129772No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20110.14525739306679455No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14830.10711920135159439No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG62900.026.2097361
TACATGG65900.024.367732
GTACCTG12950.023.8466531
TACCTGG12000.023.7549322
ACATGGG65000.023.6087513
GTATCAA92750.023.357951
GGTATCA69900.022.3615821
GAGTACT46450.022.09325412-13
CATGGGG30700.020.7372074
TCAACGC104700.020.3743364
ATCAACG105200.020.2323383
TATCAAC105800.020.207412
GTACTTT50150.020.18085714-15
CAACGCA106200.020.1767655
AACGCAG108000.019.8851976
TAAGGTG7150.019.2704335
GAGTACG1801.2315891E-418.4760591
ACGCAGA117650.018.2528347
ATGGGGA16500.018.1407875
AGTACTT50150.018.09481212-13
Produced by FastQC (version 0.11.5)