##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936431_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1384439 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.492967909745392 31.0 31.0 34.0 30.0 34.0 2 31.5744232862553 33.0 31.0 34.0 30.0 34.0 3 31.58947848189772 33.0 31.0 34.0 28.0 34.0 4 35.22175841622491 37.0 35.0 37.0 33.0 37.0 5 35.22552600728526 37.0 35.0 37.0 33.0 37.0 6 35.21370100091084 37.0 35.0 37.0 32.0 37.0 7 35.123885559421545 37.0 35.0 37.0 32.0 37.0 8 35.086608366276884 37.0 35.0 37.0 32.0 37.0 9 36.602001243825114 39.0 37.0 39.0 32.0 39.0 10-11 36.47779678266792 39.0 37.0 39.0 32.0 39.0 12-13 36.38630268289177 39.0 37.0 39.0 32.0 39.0 14-15 37.518203402244524 40.0 37.0 41.0 32.0 41.0 16-17 37.415020091170504 40.0 37.0 41.0 32.0 41.0 18-19 37.38463269237576 40.0 37.0 41.0 32.0 41.0 20-21 37.24350729790189 39.5 37.0 41.0 31.0 41.0 22-23 37.35048348103456 40.0 37.0 41.0 32.0 41.0 24-25 37.40341827989532 40.0 37.0 41.0 32.0 41.0 26-27 37.30857155858799 40.0 37.0 41.0 31.0 41.0 28-29 37.16979187959889 39.5 36.5 41.0 31.0 41.0 30-31 36.97194784313357 39.0 36.0 41.0 30.5 41.0 32-33 36.7835220620049 39.0 36.0 41.0 30.0 41.0 34-35 36.59734520625322 39.0 35.5 41.0 30.0 41.0 36-37 36.39897135229504 39.0 35.0 40.5 30.0 41.0 38-39 36.203700921456274 39.0 35.0 40.0 30.0 41.0 40-41 35.99263672866771 39.0 35.0 40.0 29.0 41.0 42-43 35.75307146071441 38.0 35.0 40.0 28.0 41.0 44-45 35.49049940084034 38.0 35.0 40.0 27.0 41.0 46-47 35.319248807639774 38.0 35.0 40.0 26.5 41.0 48-49 35.16186953704714 38.0 34.5 40.0 26.0 41.0 50-51 34.18530321668199 37.5 33.5 39.5 24.0 40.5 52-53 34.289360527982815 38.0 33.5 39.5 24.0 40.5 54-55 34.72035857123355 38.0 34.0 40.0 25.0 41.0 56-57 34.62282014592192 38.0 34.0 40.0 24.5 41.0 58-59 34.39497514877867 38.0 34.0 40.0 24.0 41.0 60-61 34.11194245466936 38.0 34.0 40.0 23.0 41.0 62-63 33.74592488365323 37.0 33.0 40.0 22.5 41.0 64-65 33.381244677446965 36.5 33.0 39.5 22.0 41.0 66-67 32.91697142308184 36.0 32.0 39.0 20.5 41.0 68-69 32.54117407845344 35.5 32.0 39.0 20.0 40.5 70-71 32.14287303377036 35.0 31.5 38.5 18.5 40.0 72-73 31.746187083721274 35.0 31.0 37.5 17.5 40.0 74-75 31.320653708830797 35.0 31.0 37.0 14.5 39.0 76-77 30.85164712927041 35.0 30.5 36.5 10.0 39.0 78-79 30.36635525292194 34.0 30.0 36.0 7.5 38.5 80-81 29.910347079214034 34.0 29.5 35.5 6.5 37.0 82-83 29.503827543142023 34.0 29.0 35.0 2.0 37.0 84-85 29.151724272430926 34.0 29.0 35.0 2.0 36.5 86-87 28.787976212747544 34.0 29.0 35.0 2.0 36.0 88-89 28.38386378887044 34.0 28.5 35.0 2.0 36.0 90-91 27.97502815219739 34.0 27.0 35.0 2.0 35.0 92-93 27.528028681653723 33.5 26.5 35.0 2.0 35.0 94-95 27.03264824235665 33.0 25.5 35.0 2.0 35.0 96-97 26.44580367932426 33.0 25.0 35.0 2.0 35.0 98-99 25.69474061334591 33.0 22.0 35.0 2.0 35.0 100-101 22.805861435570655 29.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3324352794254253 1101 2 0.4236649640860577 1101 3 0.271644117661733 1101 4 0.41626198384579993 1101 5 0.4403063739778048 1101 6 0.32362902830989526 1101 7 0.5137628499254845 1101 8 0.47289600318853076 1101 9 0.5182775222504716 1101 10-11 0.6893814645221639 1101 12-13 0.7581375573957061 1101 14-15 0.6722631446468412 1101 16-17 0.654802851804348 1101 18-19 0.5945461491618289 1101 20-21 0.7132997043952685 1101 22-23 0.5757571252800773 1101 24-25 0.5772418990396133 1101 26-27 0.5920058748817141 1101 28-29 0.6158723114980873 1101 30-31 0.616051012211706 1101 32-33 0.5127261999591965 1101 34-35 0.526769644450944 1101 36-37 0.40667674999851045 1101 38-39 0.2706585932717971 1101 40-41 0.28639442818575134 1101 42-43 0.47367919402885406 1101 44-45 0.143598786706562 1101 46-47 0.36350696963206275 1101 48-49 0.6713345806478728 1101 50-51 0.4566045606636635 1101 52-53 0.5692775558256713 1101 54-55 0.39908001166678275 1101 56-57 0.38122086714218284 1101 58-59 0.5019532679632235 1101 60-61 0.46980917082385787 1101 62-63 0.4262544919016449 1101 64-65 0.3874821630617191 1101 66-67 0.4573096297125403 1101 68-69 0.40105372531292716 1101 70-71 0.3104283587077319 1101 72-73 0.3008193123042737 1101 74-75 0.23673450988850675 1101 76-77 0.07177890415654886 1101 78-79 0.14029388027072898 1101 80-81 0.020438255069411326 1101 82-83 0.13127329923375797 1101 84-85 -0.07358341940176771 1101 86-87 -0.02655471390018249 1101 88-89 0.10681544899109596 1101 90-91 0.4154487778023288 1101 92-93 0.13721511832567046 1101 94-95 -0.0399578123678026 1101 96-97 -0.11847331935205574 1101 98-99 -0.004975088890962098 1101 100-101 0.38305804993918713 1102 1 0.28509728677105173 1102 2 0.29483557157058726 1102 3 0.36671178627013035 1102 4 0.40730582609629096 1102 5 0.4255243964151276 1102 6 0.4739603323149524 1102 7 0.3827210098899485 1102 8 0.3454525188339588 1102 9 0.3929651454336138 1102 10-11 0.47287038664225634 1102 12-13 0.46181787875120506 1102 14-15 0.4084328448777299 1102 16-17 0.3023166639590471 1102 18-19 0.33626511326587405 1102 20-21 0.30123980062672473 1102 22-23 0.23330860410352017 1102 24-25 0.35691816590983905 1102 26-27 0.06614977156742441 1102 28-29 0.12260350788409369 1102 30-31 0.35603461907680156 1102 32-33 0.2874544931335308 1102 34-35 0.25491077557725816 1102 36-37 0.19196703988168906 1102 38-39 0.10564448391332348 1102 40-41 0.2786586830176603 1102 42-43 0.08508305955508888 1102 44-45 0.0693588462724577 1102 46-47 0.1873311376178961 1102 48-49 0.25430668103392406 1102 50-51 0.4021164707141551 1102 52-53 0.4229481789525025 1102 54-55 0.413960535746547 1102 56-57 0.39938815220128276 1102 58-59 0.46176881391092905 1102 60-61 0.45367882663059333 1102 62-63 0.4258903390575597 1102 64-65 0.26031116764804096 1102 66-67 0.2646026976689697 1102 68-69 0.40623101518985294 1102 70-71 0.47027612780229333 1102 72-73 0.13099269160513316 1102 74-75 0.12478910778743568 1102 76-77 0.20480262414678663 1102 78-79 0.3589792384482102 1102 80-81 0.39386823698690776 1102 82-83 0.31857456375763604 1102 84-85 0.0467453192653835 1102 86-87 0.25267699868186 1102 88-89 0.24097636450388293 1102 90-91 0.18609880939978396 1102 92-93 0.06652598096746942 1102 94-95 -0.10592266098158021 1102 96-97 0.07692377618488067 1102 98-99 0.0908787619315099 1102 100-101 0.19816038189390994 1103 1 0.26656488046537774 1103 2 0.23508280753171462 1103 3 0.3043528824953441 1103 4 0.4998437251051584 1103 5 0.4340657428349033 1103 6 0.3416099827500574 1103 7 0.40117044679139013 1103 8 0.30626844895957106 1103 9 0.2371781902821155 1103 10-11 0.42906929453335607 1103 12-13 0.1432610318458316 1103 14-15 0.3535730245804487 1103 16-17 0.5075385910360097 1103 18-19 0.6068418471013146 1103 20-21 0.6189479798663697 1103 22-23 0.311098530717409 1103 24-25 0.47333838986291 1103 26-27 0.39738710908567754 1103 28-29 0.42478322347269426 1103 30-31 0.46558832086289215 1103 32-33 0.42170606408523525 1103 34-35 0.7452464479188592 1103 36-37 0.6611213951322412 1103 38-39 0.4242254498315461 1103 40-41 0.39608965440286426 1103 42-43 0.4647262911169108 1103 44-45 0.447572302752107 1103 46-47 0.5916548726560649 1103 48-49 0.6878890297755547 1103 50-51 0.5258701609231196 1103 52-53 0.8009567846162966 1103 54-55 0.8276594918756714 1103 56-57 0.7933230244348266 1103 58-59 0.6202095036503792 1103 60-61 0.6387874265058215 1103 62-63 0.6055808438845816 1103 64-65 0.5615563518851445 1103 66-67 0.6966256718326846 1103 68-69 0.5466802076852986 1103 70-71 0.5766261815073008 1103 72-73 0.3036838589010351 1103 74-75 0.41585156638313236 1103 76-77 0.5518945143699021 1103 78-79 0.39061952427459445 1103 80-81 0.5446789574057398 1103 82-83 0.3145634111260911 1103 84-85 0.31722300435468753 1103 86-87 0.22723336963871077 1103 88-89 0.1507632167475954 1103 90-91 0.21608806839939376 1103 92-93 0.35104028836589407 1103 94-95 0.26397681097283154 1103 96-97 0.35445900602299574 1103 98-99 0.3242160615025149 1103 100-101 0.38350663444248667 1104 1 0.2849318717447531 1104 2 0.3111091018580794 1104 3 0.21678622303196704 1104 4 0.33912836885607334 1104 5 0.3436340334945598 1104 6 0.40912756690654106 1104 7 0.509051773776541 1104 8 0.4647928437090698 1104 9 0.4297402140808515 1104 10-11 0.6388230117787259 1104 12-13 0.6553137599170569 1104 14-15 0.64620371198329 1104 16-17 0.6344435703672247 1104 18-19 0.6848144234852143 1104 20-21 0.4440227430292154 1104 22-23 0.7123637534732907 1104 24-25 0.6583721639910252 1104 26-27 0.4592453483696204 1104 28-29 0.4643261399679872 1104 30-31 0.5729433430652833 1104 32-33 0.5703638126161152 1104 34-35 0.6346656305082803 1104 36-37 0.5307916007048021 1104 38-39 0.35967399395401145 1104 40-41 0.25849789961208813 1104 42-43 0.35585753270823517 1104 44-45 0.28488800894159283 1104 46-47 0.20776816411738253 1104 48-49 0.46856876460159924 1104 50-51 0.35330911305378976 1104 52-53 0.49715170560086364 1104 54-55 0.279079469150453 1104 56-57 0.3419891380748368 1104 58-59 0.39945531935308054 1104 60-61 0.4025093423946444 1104 62-63 0.4491079921491661 1104 64-65 0.43545755994631463 1104 66-67 0.47784251637410335 1104 68-69 0.45606725431381534 1104 70-71 0.23627010669533632 1104 72-73 0.28934780458359555 1104 74-75 0.20077415426905887 1104 76-77 0.21619641115839627 1104 78-79 0.5212034088918536 1104 80-81 0.644265233050028 1104 82-83 0.32815043963019974 1104 84-85 0.2574410548660033 1104 86-87 0.0755505885187624 1104 88-89 0.15711756277033473 1104 90-91 0.32023716252778556 1104 92-93 0.013983552013222322 1104 94-95 0.013826239008491115 1104 96-97 0.20787127345553102 1104 98-99 0.19658227203836987 1104 100-101 -0.0055399522444403715 1105 1 0.2140187074894584 1105 2 0.23085191378601522 1105 3 0.25736946333103106 1105 4 0.14887685681424756 1105 5 0.06876155622687463 1105 6 0.2979593195970196 1105 7 0.38190944825808515 1105 8 0.46545404484728437 1105 9 0.2885660652034616 1105 10-11 0.4324707445617477 1105 12-13 0.14898930900719876 1105 14-15 0.1846851295624461 1105 16-17 0.2816076226769155 1105 18-19 0.1627998838263025 1105 20-21 0.20396650898130275 1105 22-23 0.00244498460801168 1105 24-25 0.009673140193044105 1105 26-27 -0.05916228081644448 1105 28-29 -0.09561743803607925 1105 30-31 0.045499924817036685 1105 32-33 -0.12299072125400556 1105 34-35 -0.14771292687788673 1105 36-37 -0.01878476445565269 1105 38-39 -0.18636406176129583 1105 40-41 -0.31668732316269654 1105 42-43 -0.20656808686241845 1105 44-45 0.1126716131080272 1105 46-47 0.18684541451300873 1105 48-49 0.21241553433895888 1105 50-51 0.18429610329177137 1105 52-53 0.1785356420594013 1105 54-55 0.2534993675833448 1105 56-57 0.1292203254176627 1105 58-59 0.22597252975337057 1105 60-61 0.29573644750645656 1105 62-63 0.24691914949549698 1105 64-65 0.08306159857339424 1105 66-67 0.18655578663208416 1105 68-69 0.421674270037542 1105 70-71 0.3050692316093446 1105 72-73 0.09932427349135153 1105 74-75 0.2834440310879245 1105 76-77 0.0665683602921412 1105 78-79 0.15586703831850102 1105 80-81 0.400665239064363 1105 82-83 0.047866562939137935 1105 84-85 -0.12244117690397971 1105 86-87 -0.18526506830318823 1105 88-89 -0.11019658482900851 1105 90-91 -0.04847926525694746 1105 92-93 -0.4327873130363429 1105 94-95 -0.15937838751999323 1105 96-97 -0.21203881221117626 1105 98-99 -0.15493822745229124 1105 100-101 -0.1692302740490419 1106 1 0.3044697191269101 1106 2 0.3188474712730951 1106 3 0.26408393529974106 1106 4 0.32505267409175786 1106 5 0.14668178414710553 1106 6 0.2534581365365156 1106 7 0.32953004799733066 1106 8 0.4646584970178367 1106 9 0.6094193174126517 1106 10-11 0.6047853179356437 1106 12-13 0.42777268821429715 1106 14-15 0.4155726663821824 1106 16-17 0.4749951594966575 1106 18-19 0.3885972424626445 1106 20-21 0.19318979354357424 1106 22-23 0.40489983515607975 1106 24-25 0.257339613706975 1106 26-27 0.3668704827163154 1106 28-29 0.5136025870737839 1106 30-31 0.5334127887892208 1106 32-33 0.39837317849973175 1106 34-35 0.36513354084146243 1106 36-37 0.17564036471523536 1106 38-39 0.2928345958271876 1106 40-41 0.327847661626528 1106 42-43 0.3497274851111243 1106 44-45 0.3690082300378634 1106 46-47 0.18040558159012932 1106 48-49 0.3861950504913594 1106 50-51 0.06769764368483777 1106 52-53 0.1738866971562203 1106 54-55 0.34780739790831916 1106 56-57 0.31168628224803285 1106 58-59 0.3799424220707195 1106 60-61 0.5695521797967658 1106 62-63 0.552737272050905 1106 64-65 0.4235832316797712 1106 66-67 0.4270460324189358 1106 68-69 0.5643199796324083 1106 70-71 0.3720274381862012 1106 72-73 0.21864497946460304 1106 74-75 0.5516378115698402 1106 76-77 0.6852371757829943 1106 78-79 0.6296000770939152 1106 80-81 0.6932077326661776 1106 82-83 0.3745403521129269 1106 84-85 0.5009828174945916 1106 86-87 0.5764852576420552 1106 88-89 0.5731144152192833 1106 90-91 0.5476957069773469 1106 92-93 0.5492802662770018 1106 94-95 0.5602411015910214 1106 96-97 0.6270866887787321 1106 98-99 0.9585583199425614 1106 100-101 0.8639575911303474 1107 1 0.22131702707010703 1107 2 0.15645598148434559 1107 3 0.26985191161566036 1107 4 0.33915649264763204 1107 5 0.3235270849650078 1107 6 0.3966702896801735 1107 7 0.4293958214932374 1107 8 0.43836390120269897 1107 9 0.7145650848871838 1107 10-11 0.5502561142626092 1107 12-13 0.5260302525677574 1107 14-15 0.3024987539932056 1107 16-17 0.46497897704896474 1107 18-19 0.3869520269475615 1107 20-21 0.5771365545451275 1107 22-23 0.5379126556688973 1107 24-25 0.43698109095119264 1107 26-27 0.45265188168264103 1107 28-29 0.3321038917052235 1107 30-31 0.46030638605952845 1107 32-33 0.2150009571138085 1107 34-35 0.32861452332929275 1107 36-37 0.27028384908474834 1107 38-39 0.15601262784083758 1107 40-41 0.31908279635481307 1107 42-43 0.3081636836158097 1107 44-45 0.24210898772766143 1107 46-47 0.29943375661145666 1107 48-49 0.35772899599790975 1107 50-51 0.33777579612599595 1107 52-53 0.43625686123871077 1107 54-55 0.6404951895687176 1107 56-57 0.30273262175327176 1107 58-59 0.48846204172047436 1107 60-61 0.4895958347719258 1107 62-63 0.5975996585228813 1107 64-65 0.6599918573007315 1107 66-67 0.6613998635193425 1107 68-69 0.7019059096840934 1107 70-71 0.4798658896890373 1107 72-73 0.4707769228636671 1107 74-75 0.4115141224735481 1107 76-77 0.46471620132366454 1107 78-79 0.5903965294999516 1107 80-81 0.6955905667270947 1107 82-83 0.50814778349433 1107 84-85 0.23083692457472793 1107 86-87 0.23041483263327223 1107 88-89 0.21301418439987074 1107 90-91 0.1252240576221375 1107 92-93 0.27260368942918944 1107 94-95 0.32156379716029093 1107 96-97 0.47412116115567216 1107 98-99 0.40552505184079735 1107 100-101 0.3966216736231978 1108 1 0.2480934994763544 1108 2 0.10625387018392018 1108 3 0.3141973911654077 1108 4 0.27665807459096214 1108 5 0.2669563473420169 1108 6 0.23910029673619704 1108 7 0.2766993241489075 1108 8 0.10873042803667232 1108 9 0.40732038389779035 1108 10-11 0.37767220243757293 1108 12-13 0.21901956817799118 1108 14-15 0.27843856154896685 1108 16-17 0.36549039435029584 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26 18189.0 27 21333.0 28 25277.0 29 31063.0 30 37351.0 31 46032.0 32 57931.0 33 74558.0 34 98981.0 35 136389.0 36 192487.0 37 253913.0 38 197768.0 39 27538.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.56376236493657 18.430392622179262 14.199169662858848 22.806675350025323 2 15.666346161486933 19.904960943397725 40.21706700918099 24.21162588593435 3 19.739463756536164 24.232095718750315 29.510078585660395 26.518361939053133 4 12.86880644664891 16.283582617918 36.95805617988081 33.88955475555228 5 13.888726315812292 37.28161919894104 33.87242565533207 14.957228829914602 6 32.11964058968961 35.54528366111131 17.553593199736074 14.781482549463002 7 28.974729236661567 32.59620017524762 20.283719092382075 18.145351495708745 8 26.65091613201549 34.42922374429224 19.562453037396683 19.357407086295588 9 26.558654486754275 16.99038526849134 20.339788918129543 36.11117132662484 10-11 25.29313807776284 26.447010773838276 26.868693145954275 21.39115800244461 12-13 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26.3731985391843 28.723115328163463 22.269204988038418 38-39 22.383342554885086 26.636548548941878 28.81523044007749 22.16487845609554 40-41 22.507965423812553 26.617156197428383 28.71424227167892 22.160636107080144 42-43 22.29905191873589 26.70786455981941 28.397002257336347 22.596081264108353 44-45 22.490442696811826 26.781396440728468 28.263288579090407 22.4648722833693 46-47 22.751344153496607 26.969423690689574 27.798759265151546 22.480472890662273 48-49 22.7996853571911 27.110710785039586 27.593040297538256 22.496563560231056 50-51 22.602131422683286 27.33125033182712 27.66389009380008 22.40272815168951 52-53 22.927726029969985 27.285003810356223 27.39649736164434 22.39077279802945 54-55 22.454468031006453 27.20967207158269 27.56456607652506 22.771293820885795 56-57 22.636417744852732 27.54362102939562 27.30157090602301 22.51839031972864 58-59 22.93709299343733 27.144642412115477 27.29810849174158 22.620156102705614 60-61 22.695786749688036 27.11136553662012 27.85339311931493 22.339454594376914 62-63 22.596961419848395 27.260615293158665 27.74467750073225 22.397745786260696 64-65 22.38594879307032 27.406274965292376 27.579415813964236 22.628360427673062 66-67 22.382318337687934 27.51482207549153 27.31076454925827 22.792095037562262 68-69 22.4307004352473 27.37620961243985 27.520051509924404 22.673038442388446 70-71 22.533586629599743 27.398204786200868 27.325352597726187 22.7428559864732 72-73 22.558407847483256 27.265279524689184 27.47648623128778 22.69982639653978 74-75 22.42952263621288 27.753322904285184 27.194850988652085 22.622303470849847 76-77 22.62957070356755 27.433036230376178 27.14214700238145 22.795246063674828 78-79 22.601152873347637 27.518120478914764 27.22377675700074 22.656949890736865 80-81 22.592560729988854 27.714505072821044 27.065455802043587 22.627478395146518 82-83 22.574827363164303 27.672880749592572 27.1498570845243 22.602434802718825 84-85 22.55397236224924 27.580531839765342 27.075784367024706 22.78971143096071 86-87 22.627996420857716 27.52670358755912 27.10665648939894 22.73864350218422 88-89 22.56321686693454 27.488350771202608 26.915706147910072 23.032726213952774 90-91 22.550035896711414 27.434843042668128 27.140985011542227 22.87413604907823 92-93 22.615365774269378 27.447403206618866 27.27313122745058 22.664099791661172 94-95 22.416996062421866 27.33786703259907 27.22903368776714 23.01610321721192 96-97 22.300938661021817 27.357643034275487 27.330370027869037 23.01104827683366 98-99 22.275709878807216 27.506595519491338 26.92590795216753 23.291786649533915 100-101 22.25890663113158 27.364039087147336 27.118090907218082 23.258963374502997 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 144.0 1 111.5 2 124.5 3 186.5 4 185.5 5 184.5 6 293.0 7 519.5 8 818.0 9 1037.5 10 1045.5 11 1025.5 12 1102.0 13 1187.0 14 1311.5 15 1481.5 16 1654.5 17 1781.0 18 1901.5 19 2072.5 20 2273.5 21 2622.0 22 3149.0 23 3715.0 24 4462.0 25 5561.5 26 7069.0 27 8820.5 28 10612.5 29 12881.0 30 15727.5 31 18955.0 32 22260.0 33 24593.5 34 27079.5 35 29421.0 36 32046.0 37 34960.5 38 36512.0 39 37569.0 40 39007.0 41 41584.5 42 43984.0 43 46091.5 44 48361.0 45 50497.5 46 51564.0 47 53471.0 48 59470.5 49 59788.0 50 53832.5 51 50421.0 52 47837.0 53 46304.5 54 45201.0 55 42244.5 56 38550.5 57 34693.0 58 31391.0 59 27858.0 60 23349.5 61 19272.0 62 16179.5 63 12803.0 64 9499.5 65 7481.5 66 5822.0 67 4594.5 68 3542.5 69 2548.0 70 1890.0 71 1495.5 72 1259.5 73 1113.5 74 881.0 75 671.0 76 509.0 77 341.5 78 212.5 79 140.5 80 93.0 81 56.5 82 36.0 83 25.5 84 19.5 85 13.5 86 10.5 87 9.0 88 6.5 89 6.5 90 5.0 91 4.0 92 3.0 93 1.5 94 2.0 95 1.0 96 0.5 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013146119113951572 2 0.011990416334703081 3 0.015674218943557642 4 0.020080335789442513 5 0.03221521497155166 6 0.05258447645580629 7 0.00808991945473943 8 0.02593108110938799 9 0.037632571749278955 10-11 0.04171364718850018 12-13 0.03170959500563044 14-15 0.027700750990112242 16-17 0.03109562790415468 18-19 0.024594799770881925 20-21 0.05511257628541236 22-23 0.013110003402100058 24-25 0.0029976040836757704 26-27 0.0015890913214666733 28-29 7.223142370303061E-5 30-31 0.0026003312533091024 32-33 0.002130826999239403 34-35 0.0 36-37 5.056199659212143E-4 38-39 7.223142370303061E-5 40-41 0.0013001656266545512 42-43 0.004622811116993959 44-45 0.0019141327281303115 46-47 0.0012640499148030358 48-49 8.667770844363674E-4 50-51 0.0042255382866272905 52-53 0.003900496879963653 54-55 0.00592297674364851 56-57 0.001083471355545459 58-59 0.0067897538280848775 60-61 0.00408107543922123 62-63 6.861985251787908E-4 64-65 0.0012279342029515204 66-67 0.01953860011166978 68-69 0.045361334085503226 70-71 0.05890472602982146 72-73 0.018057855925757652 74-75 0.03496000907226682 76-77 0.029398189447133457 78-79 0.0615772887068336 80-81 0.08559423708809127 82-83 0.05446249347208508 84-85 0.020333145772403118 86-87 0.022283394212384944 88-89 0.024161411228663738 90-91 0.0438805898995911 92-93 0.02856752807454861 94-95 0.015349177536894005 96-97 0.02069430289091827 98-99 0.05305398070987599 100-101 0.07349547361783365 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1384439.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.56709261046475 #Duplication Level Percentage of deduplicated Percentage of total 1 83.35886948916969 55.489575851892035 2 10.328296825896608 13.750493826356575 3 2.813608754425429 5.6188126357635575 4 1.1786427191767994 3.1383527616836804 5 0.6145734138325746 2.0455182677261234 6 0.3783494459389946 1.511137356416345 7 0.24448946684657025 1.1392467087301155 8 0.17768071035694902 0.9462150641139351 9 0.13575968988190645 0.8133415064223156 >10 0.6951707078732378 8.589612397697538 >50 0.049446568281227204 2.2772761148860243 >100 0.02293802823283763 2.93050101666901 >500 0.0015219260609951207 0.6697327404744688 >1k 6.52254026140766E-4 1.0801837511682761 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4294 0.31016173338081343 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3615 0.26111659668645565 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2474 0.17870054224129772 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2011 0.14525739306679455 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1483 0.10711920135159439 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 7.223142370303061E-5 10-11 0.0 0.0 0.0 0.0 7.223142370303061E-5 12-13 7.223142370303061E-5 0.0 0.0 0.0 7.223142370303061E-5 14-15 7.223142370303061E-5 0.0 0.0 0.0 7.223142370303061E-5 16-17 1.0834713555454591E-4 0.0 0.0 3.6115711851515304E-5 7.223142370303061E-5 18-19 1.4446284740606122E-4 0.0 0.0 7.223142370303061E-5 7.223142370303061E-5 20-21 1.4446284740606122E-4 0.0 0.0 7.223142370303061E-5 7.223142370303061E-5 22-23 1.4446284740606122E-4 0.0 0.0 2.8892569481212243E-4 1.0834713555454591E-4 24-25 1.4446284740606122E-4 0.0 0.0 5.778513896242449E-4 1.4446284740606122E-4 26-27 1.4446284740606122E-4 0.0 0.0 6.861985251787908E-4 1.4446284740606122E-4 28-29 1.4446284740606122E-4 0.0 0.0 0.0012279342029515204 1.4446284740606122E-4 30-31 1.4446284740606122E-4 0.0 0.0 0.0024197526940515257 1.4446284740606122E-4 32-33 1.4446284740606122E-4 0.0 0.0 0.003900496879963653 3.6115711851515307E-4 34-35 1.4446284740606122E-4 1.4446284740606122E-4 0.0 0.005236778218469719 4.333885422181837E-4 36-37 1.4446284740606122E-4 1.4446284740606122E-4 0.0 0.008017688031036398 4.333885422181837E-4 38-39 1.4446284740606122E-4 1.4446284740606122E-4 0.0 0.01231545774136672 4.333885422181837E-4 40-41 1.4446284740606122E-4 1.4446284740606122E-4 0.0 0.01679380601095462 4.333885422181837E-4 42-43 2.1669427110909182E-4 1.4446284740606122E-4 0.0 0.023872485533851618 4.333885422181837E-4 44-45 2.8892569481212243E-4 1.4446284740606122E-4 0.0 0.03109562790415468 4.69504254069699E-4 46-47 2.8892569481212243E-4 1.4446284740606122E-4 0.0 0.04445844128921535 5.056199659212143E-4 48-49 2.8892569481212243E-4 1.4446284740606122E-4 0.0 0.060746627334248744 5.056199659212143E-4 50-51 2.8892569481212243E-4 1.4446284740606122E-4 0.0 0.06811423255195787 5.056199659212143E-4 52-53 2.8892569481212243E-4 1.4446284740606122E-4 0.0 0.08032134315777004 5.778513896242449E-4 54-55 2.8892569481212243E-4 1.4446284740606122E-4 0.0 0.10484391150494893 6.861985251787908E-4 56-57 2.8892569481212243E-4 1.4446284740606122E-4 0.0 0.14070681337350363 7.223142370303061E-4 58-59 2.8892569481212243E-4 1.4446284740606122E-4 0.0 0.17895335222425834 7.223142370303061E-4 60-61 2.8892569481212243E-4 1.4446284740606122E-4 0.0 0.22713171183417974 7.223142370303061E-4 62-63 2.8892569481212243E-4 1.4446284740606122E-4 0.0 0.2722402359367224 7.223142370303061E-4 64-65 2.8892569481212243E-4 1.4446284740606122E-4 0.0 0.3156513215822438 7.223142370303061E-4 66-67 3.6115711851515307E-4 1.4446284740606122E-4 0.0 0.3860047282689956 7.223142370303061E-4 68-69 3.6115711851515307E-4 1.4446284740606122E-4 0.0 0.4870203743176839 7.223142370303061E-4 70-71 3.6115711851515307E-4 1.4446284740606122E-4 0.0 0.5372212137912902 7.223142370303061E-4 72-73 3.972728303666684E-4 1.4446284740606122E-4 0.0 0.6082608190032208 7.223142370303061E-4 74-75 4.333885422181837E-4 1.4446284740606122E-4 0.0 0.6834898467899272 7.223142370303061E-4 76-77 4.333885422181837E-4 1.4446284740606122E-4 0.0 0.7605607758810609 7.223142370303061E-4 78-79 4.333885422181837E-4 1.4446284740606122E-4 0.0 0.8453243515965673 7.223142370303061E-4 80-81 6.500828133272755E-4 1.4446284740606122E-4 0.0 0.9807582710397498 7.945456607333368E-4 82-83 6.500828133272755E-4 1.4446284740606122E-4 0.0 1.1417259987619532 0.0010112399318424287 84-85 6.500828133272755E-4 1.4446284740606122E-4 0.0 1.366112916495418 0.0010112399318424287 86-87 6.500828133272755E-4 1.4446284740606122E-4 0.0 1.6796334110784223 0.0010834713555454593 88-89 6.500828133272755E-4 1.4446284740606122E-4 0.0 2.0201323424145086 0.0011557027792484897 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 6290 0.0 26.209736 1 TACATGG 6590 0.0 24.36773 2 GTACCTG 1295 0.0 23.846653 1 TACCTGG 1200 0.0 23.754932 2 ACATGGG 6500 0.0 23.608751 3 GTATCAA 9275 0.0 23.35795 1 GGTATCA 6990 0.0 22.361582 1 GAGTACT 4645 0.0 22.093254 12-13 CATGGGG 3070 0.0 20.737207 4 TCAACGC 10470 0.0 20.374336 4 ATCAACG 10520 0.0 20.232338 3 TATCAAC 10580 0.0 20.20741 2 GTACTTT 5015 0.0 20.180857 14-15 CAACGCA 10620 0.0 20.176765 5 AACGCAG 10800 0.0 19.885197 6 TAAGGTG 715 0.0 19.270433 5 GAGTACG 180 1.2315891E-4 18.476059 1 ACGCAGA 11765 0.0 18.252834 7 ATGGGGA 1650 0.0 18.140787 5 AGTACTT 5015 0.0 18.094812 12-13 >>END_MODULE