FastQCFastQC Report
Thu 26 May 2016
SRR936431_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936431_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1384439
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59010.42623763127158365No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52910.382176462812735No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30120.21756104819352823No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27120.195891621082619No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16080.11614812931447323No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14430.10422994440347318No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG1056.908216E-527.1420632
GTATCAA108850.023.828251
GTACATG63300.022.9639021
CCGTATG1252.2577781E-422.7976869
GGTATCA82950.022.5635681
GAGTACT66600.022.25240312-13
TACATGG65200.022.1465912
ACATGGG65450.020.9734123
GTACTTT70650.020.84307514-15
AGTACTT69600.020.54252412-13
TATCAAC128550.020.2113882
ATCAACG129300.020.1308883
TCAACGC129550.020.0920414
CAACGCA130500.019.9821745
AACGCAG132300.019.7813996
GTAAGGT6100.019.4666444
TAGAACG1801.2338287E-418.4716824
AGAGTAC112000.018.40571210-11
GTACCTG12300.018.1518421
TACTTTT78900.018.09171714-15
Produced by FastQC (version 0.11.5)