##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936431_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1384439 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.991965698741513 31.0 31.0 34.0 30.0 34.0 2 32.1096082962124 33.0 31.0 34.0 30.0 34.0 3 32.220782569690684 34.0 31.0 34.0 30.0 34.0 4 35.94386823832614 37.0 35.0 37.0 35.0 37.0 5 35.80995695729462 37.0 35.0 37.0 35.0 37.0 6 35.86378236960964 37.0 35.0 37.0 35.0 37.0 7 35.79533153862322 37.0 35.0 37.0 33.0 37.0 8 35.76577082847276 37.0 35.0 37.0 33.0 37.0 9 37.389648081280576 39.0 37.0 39.0 34.0 39.0 10-11 37.29747970116416 39.0 37.0 39.0 34.0 39.0 12-13 37.176471119348705 39.0 37.0 39.0 33.0 39.0 14-15 38.294671704567705 40.0 38.0 41.0 33.0 41.0 16-17 38.2635479064083 40.0 38.0 41.0 33.0 41.0 18-19 38.41387991814735 40.0 38.0 41.0 33.5 41.0 20-21 38.424521412644395 40.0 38.0 41.0 34.0 41.0 22-23 38.332726107831405 40.0 38.0 41.0 34.0 41.0 24-25 38.21712729849419 40.0 38.0 41.0 33.0 41.0 26-27 38.017546096288825 40.0 37.0 41.0 33.0 41.0 28-29 38.036259452384684 40.0 37.0 41.0 33.0 41.0 30-31 37.9034179187382 40.0 37.0 41.0 33.0 41.0 32-33 37.70661293130286 40.0 37.0 41.0 33.0 41.0 34-35 37.52054803425792 40.0 36.0 41.0 32.5 41.0 36-37 37.33613976491561 40.0 36.0 41.0 32.0 41.0 38-39 37.13094437530292 39.0 36.0 41.0 31.0 41.0 40-41 36.90631114841463 39.0 36.0 41.0 31.0 41.0 42-43 36.63392103227372 39.0 35.0 41.0 30.5 41.0 44-45 36.334455328114856 39.0 35.0 40.0 30.0 41.0 46-47 35.92011674042699 39.0 35.0 40.0 29.5 41.0 48-49 35.50591900401535 38.5 35.0 40.0 27.5 41.0 50-51 35.19687324613074 38.0 34.5 40.0 27.0 41.0 52-53 34.92233316166332 38.0 34.0 40.0 26.0 41.0 54-55 34.59677782842003 38.0 34.0 40.0 25.5 41.0 56-57 34.27470657789907 38.0 33.5 40.0 24.5 41.0 58-59 33.90544039860188 37.0 33.0 40.0 23.5 41.0 60-61 33.52694593261241 37.0 33.0 39.5 23.0 41.0 62-63 33.199931163453215 36.0 32.0 39.0 22.0 40.0 64-65 32.88567246371996 36.0 32.0 39.0 22.0 40.0 66-67 32.44804429808753 35.0 31.0 39.0 20.5 40.0 68-69 32.005778152739126 35.0 31.0 38.0 19.5 40.0 70-71 31.490259953670765 35.0 30.5 37.5 18.0 39.5 72-73 30.923964147210533 34.0 30.0 37.0 14.5 39.0 74-75 30.393578193044256 34.0 29.0 36.0 11.0 39.0 76-77 29.02434451788775 32.5 28.0 35.0 9.0 37.0 78-79 29.800500419303415 34.0 29.0 35.0 7.5 37.0 80-81 29.648751949345545 34.0 29.0 35.0 7.0 37.0 82-83 29.24910776133871 34.0 29.0 35.0 3.5 36.5 84-85 28.798408958430095 33.5 29.0 35.0 2.0 36.0 86-87 28.374349465740274 33.0 28.0 35.0 2.0 36.0 88-89 27.88638285977208 33.0 27.0 35.0 2.0 35.0 90-91 27.32355055007841 32.5 26.0 34.5 2.0 35.0 92-93 26.74872782405003 32.0 25.0 34.0 2.0 35.0 94-95 26.01684906305009 32.0 24.5 34.0 2.0 35.0 96-97 25.19877329373125 31.5 21.5 34.0 2.0 35.0 98-99 24.042610400313773 31.0 9.5 34.0 2.0 35.0 100-101 20.216254742895856 26.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3387591921003832 1101 2 0.36425340777624626 1101 3 0.2236355219765187 1101 4 0.2195132485410909 1101 5 0.17448300141275297 1101 6 0.22885906805645106 1101 7 0.284495245100544 1101 8 0.18546896869067808 1101 9 0.2715581157065756 1101 10-11 0.2946312588872573 1101 12-13 0.40598032509316084 1101 14-15 0.4345065973500368 1101 16-17 0.4655167632559838 1101 18-19 0.3950935106829192 1101 20-21 0.28349184088965984 1101 22-23 0.33263914257086924 1101 24-25 0.33594635291630937 1101 26-27 0.2566247374586439 1101 28-29 0.37695055759103013 1101 30-31 0.5566482390313254 1101 32-33 0.5267963017593331 1101 34-35 0.5174056996942085 1101 36-37 0.5145939515934046 1101 38-39 0.32183542821796607 1101 40-41 0.32811072668128816 1101 42-43 0.49483040891604446 1101 44-45 0.6239162952865627 1101 46-47 0.5041398781590516 1101 48-49 0.6850091258116962 1101 50-51 0.6083674146248939 1101 52-53 0.5888569138649231 1101 54-55 0.7686755953139865 1101 56-57 0.6369651068047091 1101 58-59 0.6293624715892392 1101 60-61 0.5715177093645849 1101 62-63 0.389959363412693 1101 64-65 0.4299253797097151 1101 66-67 0.429633030237099 1101 68-69 0.4826629218320768 1101 70-71 0.40934764832177706 1101 72-73 0.528365300534503 1101 74-75 0.3944469850798846 1101 76-77 0.37250740918266345 1101 78-79 0.5592407284370005 1101 80-81 0.596209042588363 1101 82-83 0.586432283612794 1101 84-85 0.6584407739960696 1101 86-87 0.6058864971909124 1101 88-89 0.23818745914509165 1101 90-91 0.3762759787532062 1101 92-93 0.27485645112682633 1101 94-95 0.20151878199899897 1101 96-97 0.24164635523741396 1101 98-99 -0.19864999559164076 1101 100-101 0.24257816857234715 1102 1 0.20403202517196917 1102 2 0.35257674046302867 1102 3 0.30502243124489326 1102 4 0.1673843309259837 1102 5 0.13863647118869693 1102 6 0.11684765543123632 1102 7 0.22915402387456396 1102 8 0.10692583034388292 1102 9 0.26598757829956554 1102 10-11 0.26599614379118464 1102 12-13 0.21648037827700506 1102 14-15 0.31155808555886466 1102 16-17 0.3297293609421814 1102 18-19 0.281675542055126 1102 20-21 0.18454800927593595 1102 22-23 0.1329119443578506 1102 24-25 0.17283309637625877 1102 26-27 0.23901306704825487 1102 28-29 0.36370809561746853 1102 30-31 0.4497975354404744 1102 32-33 0.3036344289375279 1102 34-35 0.3665200324247948 1102 36-37 0.39541898929135755 1102 38-39 0.4160455857046941 1102 40-41 0.4348509956037816 1102 42-43 0.5560922423192451 1102 44-45 0.5468203829335252 1102 46-47 0.3870124934897632 1102 48-49 0.6599698636279072 1102 50-51 0.6341525018776721 1102 52-53 0.7031039246205708 1102 54-55 0.6683383472332949 1102 56-57 0.6881592403149028 1102 58-59 0.662423420263714 1102 60-61 0.5102752724187241 1102 62-63 0.283101464361863 1102 64-65 0.3415463604196276 1102 66-67 0.5003550164343338 1102 68-69 0.32871824553392415 1102 70-71 0.13007151159552421 1102 72-73 0.10400428866512001 1102 74-75 0.18597010753409648 1102 76-77 0.32285810964505046 1102 78-79 0.33784893876968525 1102 80-81 0.40697595348353843 1102 82-83 0.7104034892590398 1102 84-85 0.49778085646229897 1102 86-87 0.500104475829783 1102 88-89 0.287208250895965 1102 90-91 0.17221554940323358 1102 92-93 0.3816724288021298 1102 94-95 0.3629229603713213 1102 96-97 0.3197903019785002 1102 98-99 0.4045410344853799 1102 100-101 0.21279459549463198 1103 1 0.1845245675731917 1103 2 0.1773350679542034 1103 3 0.14231878637967554 1103 4 0.05707490007036853 1103 5 0.10328957546644091 1103 6 0.15492996879831367 1103 7 0.12405558614342738 1103 8 0.16848076702343207 1103 9 0.045449113297863164 1103 10-11 0.2542013033304187 1103 12-13 0.20116705622064757 1103 14-15 0.3246241587906198 1103 16-17 0.29755202509618073 1103 18-19 0.22996695118433053 1103 20-21 0.17303359253798334 1103 22-23 0.11058256225479823 1103 24-25 0.11959117946143749 1103 26-27 0.11187531854202604 1103 28-29 0.15778060701772034 1103 30-31 0.2999965976932586 1103 32-33 0.1572638579922554 1103 34-35 0.33173720001656193 1103 36-37 0.36928652124100836 1103 38-39 0.26102239929366533 1103 40-41 0.2579499350103944 1103 42-43 0.3492273280864566 1103 44-45 0.2624771597329598 1103 46-47 0.21037899314566033 1103 48-49 0.12869908107848715 1103 50-51 0.09743757574616296 1103 52-53 -0.0453871899350915 1103 54-55 0.11452304098614974 1103 56-57 0.1294808979021056 1103 58-59 0.03850186172525838 1103 60-61 -0.07319677203744845 1103 62-63 0.07354776257577811 1103 64-65 0.27880316037331454 1103 66-67 -0.026610039939846786 1103 68-69 0.02500488231445175 1103 70-71 -0.19178063563979464 1103 72-73 0.1486225329296218 1103 74-75 0.1297162082936829 1103 76-77 -0.10908414622230111 1103 78-79 -0.28250815125533535 1103 80-81 -0.10623869286741439 1103 82-83 -0.2228678850101531 1103 84-85 -0.09925668558518197 1103 86-87 -0.10071814330206408 1103 88-89 -0.24182267932612334 1103 90-91 -0.20451603492647763 1103 92-93 -0.16820858406777717 1103 94-95 -0.1396918622092187 1103 96-97 0.0891282833135989 1103 98-99 0.29101509207515264 1103 100-101 0.4246820529647728 1104 1 0.27950759337258546 1104 2 0.3275759133878964 1104 3 0.23956108873654358 1104 4 0.19487961659773134 1104 5 0.16093772503459292 1104 6 0.18453548419360288 1104 7 0.24121939589296204 1104 8 0.17925851304477902 1104 9 0.2536476173375135 1104 10-11 0.32551908685532993 1104 12-13 0.2085321862632341 1104 14-15 0.27010177166731353 1104 16-17 0.3789138648625112 1104 18-19 0.295767248145701 1104 20-21 0.254429241287049 1104 22-23 0.16707841804031887 1104 24-25 0.11954133735287087 1104 26-27 0.23096066356792022 1104 28-29 0.3427781323396033 1104 30-31 0.4223039039189871 1104 32-33 0.37337505817629335 1104 34-35 0.37581470275264905 1104 36-37 0.4270033344132713 1104 38-39 0.3787494597246024 1104 40-41 0.4169894810638084 1104 42-43 0.3587273036882195 1104 44-45 0.46079960680621923 1104 46-47 0.43565939857461444 1104 48-49 0.41765504574984647 1104 50-51 0.2490530874468604 1104 52-53 0.3734301230524366 1104 54-55 0.2257605194634209 1104 56-57 0.2997101716015038 1104 58-59 0.4849330970427488 1104 60-61 0.22885965062363312 1104 62-63 0.11439633318774867 1104 64-65 0.3237840948954229 1104 66-67 0.3768478870466936 1104 68-69 0.3407389554952154 1104 70-71 0.07522981377569238 1104 72-73 0.37219994155823244 1104 74-75 0.45815060841079713 1104 76-77 0.14840646045723815 1104 78-79 0.31897177596821535 1104 80-81 0.3165022048482129 1104 82-83 0.3171176851765942 1104 84-85 0.06505845595943782 1104 86-87 0.312537069206563 1104 88-89 0.3023701922067161 1104 90-91 0.3804069015056619 1104 92-93 0.4030771332054748 1104 94-95 0.24169855834664133 1104 96-97 0.651794801267215 1104 98-99 0.5495471548413526 1104 100-101 0.32503861574143045 1105 1 0.1074648615105076 1105 2 0.13087891191062795 1105 3 0.13609795849983897 1105 4 0.08830181175544283 1105 5 0.12531368122014896 1105 6 0.057343540397575055 1105 7 0.01803112084230918 1105 8 0.04103326861837786 1105 9 0.15886768567541054 1105 10-11 0.1434444414192768 1105 12-13 0.03082507694139025 1105 14-15 0.0902714135511502 1105 16-17 0.10839482760745511 1105 18-19 0.13826774620796556 1105 20-21 0.005318611322877587 1105 22-23 0.08571477996806465 1105 24-25 -0.06776371356224331 1105 26-27 0.07978918355242826 1105 28-29 -0.13732907321163168 1105 30-31 -0.01626791665831462 1105 32-33 0.009000793522922379 1105 34-35 -0.2205268890167318 1105 36-37 -0.04224197910282612 1105 38-39 -0.20938820092396782 1105 40-41 -0.20063835178274303 1105 42-43 -0.04014388049707662 1105 44-45 -0.10580973571401131 1105 46-47 -0.08781643694830876 1105 48-49 0.021453287308162317 1105 50-51 0.14926246154235656 1105 52-53 0.22954106501033067 1105 54-55 0.08892045195248954 1105 56-57 0.34059149746202166 1105 58-59 0.11105260943040918 1105 60-61 -0.09240478848307987 1105 62-63 -0.02205750113982674 1105 64-65 -0.12488654875136973 1105 66-67 -0.12932056700565653 1105 68-69 -0.1479982593406497 1105 70-71 -0.11262009845258092 1105 72-73 -0.31234885162752235 1105 74-75 -0.28399899069607315 1105 76-77 -0.2106592123402926 1105 78-79 -0.09141254014532052 1105 80-81 0.027493029225823307 1105 82-83 -0.11872099527793623 1105 84-85 -0.02073887593045498 1105 86-87 0.0316058995308488 1105 88-89 -0.12494072268666301 1105 90-91 0.009129118089440169 1105 92-93 0.08616463052721812 1105 94-95 0.25964966178947435 1105 96-97 0.3575766462401688 1105 98-99 0.5933347209520683 1105 100-101 0.25453574201619134 1106 1 0.1042657693283644 1106 2 0.20116719236840197 1106 3 0.06903291431615344 1106 4 0.14563195806863405 1106 5 0.230947705074513 1106 6 0.17426372002962154 1106 7 0.17818008988957956 1106 8 0.1854921995179808 1106 9 0.22527359614912257 1106 10-11 0.16572521306444798 1106 12-13 0.11847959912980599 1106 14-15 0.247830928737784 1106 16-17 0.18254314476424582 1106 18-19 0.0805687988903756 1106 20-21 0.14643177468180113 1106 22-23 0.14371552843491742 1106 24-25 0.08975475666808563 1106 26-27 0.20635794592627832 1106 28-29 0.2500942692964614 1106 30-31 0.08879208141015482 1106 32-33 0.15025399261510586 1106 34-35 0.18907280193889164 1106 36-37 0.22965188346900334 1106 38-39 0.2756786782107099 1106 40-41 0.2955323952354689 1106 42-43 0.32704067694171357 1106 44-45 0.2043356117194648 1106 46-47 0.2524661696478603 1106 48-49 0.35556768201578137 1106 50-51 0.23305815423635323 1106 52-53 0.12909379570712787 1106 54-55 0.27563576896441333 1106 56-57 0.20499767352074372 1106 58-59 0.2737228252785897 1106 60-61 0.14307736903297297 1106 62-63 0.3198258587520044 1106 64-65 0.25598891602240315 1106 66-67 -0.05925682660292608 1106 68-69 0.14393640674655828 1106 70-71 0.20133337588224975 1106 72-73 0.33233160131564077 1106 74-75 0.47889757601405236 1106 76-77 0.3041833681802544 1106 78-79 0.1099129705022257 1106 80-81 0.10913808693020144 1106 82-83 0.20343543429374833 1106 84-85 0.5034969635596198 1106 86-87 0.479457075348126 1106 88-89 0.4264028960298383 1106 90-91 0.5583414170898777 1106 92-93 0.3479566050310545 1106 94-95 0.3183433456950233 1106 96-97 0.38193260258764994 1106 98-99 0.6943306647446228 1106 100-101 0.11692407559096196 1107 1 0.11376198087937439 1107 2 0.17072913727280792 1107 3 0.020553311726956736 1107 4 0.0679436646269167 1107 5 0.0771479660008012 1107 6 0.06285321272870448 1107 7 0.04801676006871958 1107 8 0.04566581585598328 1107 9 0.036995207870731406 1107 10-11 0.07469392699891841 1107 12-13 0.1652211566590367 1107 14-15 0.25114770328743674 1107 16-17 0.27538335250171286 1107 18-19 0.2559984491487839 1107 20-21 0.13902736402396698 1107 22-23 0.1478106912629542 1107 24-25 0.06907885722506535 1107 26-27 0.05209217744818062 1107 28-29 0.17617435359572653 1107 30-31 0.21707561294711297 1107 32-33 0.32291882777993663 1107 34-35 0.2152206267168566 1107 36-37 0.21145478376504911 1107 38-39 0.29536105063280615 1107 40-41 0.20557805733592005 1107 42-43 0.261205712819077 1107 44-45 0.19932783410894928 1107 46-47 0.22937607481118505 1107 48-49 0.25547861583082465 1107 50-51 0.12535957427750333 1107 52-53 0.06631656152578813 1107 54-55 0.1685336815541163 1107 56-57 0.09680170282477008 1107 58-59 0.08497840759581266 1107 60-61 0.14252039176366793 1107 62-63 -0.022931555067053466 1107 64-65 -0.03010421459127599 1107 66-67 0.011045245753940947 1107 68-69 0.07979997240464698 1107 70-71 -0.05504740087523885 1107 72-73 0.04621902667566857 1107 74-75 0.07773218888975819 1107 76-77 -0.07535775279922419 1107 78-79 0.02555098545530754 1107 80-81 -0.04679234108926522 1107 82-83 0.24190763560841333 1107 84-85 -0.049461037343583314 1107 86-87 0.3051608236546137 1107 88-89 0.1870394056389486 1107 90-91 0.042867108293648926 1107 92-93 0.07601872555892797 1107 94-95 0.44693798165267395 1107 96-97 0.307461354657498 1107 98-99 0.7419484579103646 1107 100-101 0.5036619952055936 1108 1 0.06495331187431219 1108 2 0.22208845399762822 1108 3 0.02990198624716811 1108 4 0.08056501910789393 1108 5 0.11778115186370997 1108 6 -0.0145041492692215 1108 7 0.16239270618184065 1108 8 0.09023465470291825 1108 9 0.07469497123421576 1108 10-11 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-1.3750802305354775 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 17.0 5 36.0 6 95.0 7 241.0 8 529.0 9 1006.0 10 1621.0 11 2792.0 12 3857.0 13 5607.0 14 7229.0 15 9328.0 16 12177.0 17 15969.0 18 14323.0 19 9408.0 20 8433.0 21 8946.0 22 9690.0 23 10718.0 24 12363.0 25 14856.0 26 18003.0 27 22391.0 28 27707.0 29 34501.0 30 42319.0 31 52333.0 32 65285.0 33 84169.0 34 111083.0 35 152054.0 36 215000.0 37 266638.0 38 138193.0 39 5517.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.40166483539108 19.02749514007633 14.923704074534 22.647135949998592 2 15.934205251010436 20.30694102816937 39.54713737218316 24.21171634863704 3 19.896760286950347 24.101763208897165 30.057983849471704 25.94349265468078 4 13.223156016714398 16.876343056914912 36.574053675326034 33.32644725104465 5 13.695809992306943 37.279691410863464 33.977094191199576 15.047404405630019 6 32.2115311689428 35.87893724461677 16.99403151745942 14.91550006898101 7 28.702281805508406 32.86320001733565 20.17313984816856 18.261378328987384 8 26.873377920285236 34.17901186858527 19.315217807428574 19.632392403700923 9 26.42214849790926 16.58970397352859 20.267950076457073 36.72019745210508 10-11 25.290460612565813 26.435689835377364 26.71667007358215 21.557179478474676 12-13 26.463498933503026 23.7624048441282 27.55563805989285 22.218458162475923 14-15 23.011030897564666 25.150504232971056 26.368226045457714 25.470238824006568 16-17 22.111330102280554 28.360415883372937 27.182125408621992 22.34612860572452 18-19 21.904305117593374 26.674422073510712 29.889978442087806 21.53129436680811 20-21 22.650296511921873 25.497749976524638 30.161763324978512 21.69019018657498 22-23 22.2211708484057 25.592914639361126 30.08977689045038 22.096137621782795 24-25 22.331869919403886 25.723472552682182 30.22870718286318 21.715950345050757 26-27 22.149581382179182 25.855817202227332 30.089343177045976 21.90525823854751 28-29 21.753375738025106 26.242453616557764 30.17563805674216 21.82853258867497 30-31 21.99396575635351 25.82365908907046 30.072766304159394 22.10960885041663 32-33 21.90843413875338 25.9261921212316 30.050422875321036 22.114950864693984 34-35 22.505541115128427 25.881964586703422 29.72612787922193 21.886366418946228 36-37 22.40363386702234 25.630724191096565 29.854765947195954 22.11087599468514 38-39 22.191666531224776 25.95055829148179 29.924459541700756 21.933315635592685 40-41 22.26879951922695 25.984642132505847 29.75585021203565 21.99070813623155 42-43 22.158741440839176 26.037726581424486 29.426357899420587 22.377174078315758 44-45 22.469908629720297 26.111934190861234 29.154191049049853 22.26396613036861 46-47 22.66744869221396 26.527387627768363 28.583130062068463 22.22203361794922 48-49 22.76072168318101 26.62632435292482 28.29025518228983 22.322698781604338 50-51 22.611703979578422 26.81274730608819 28.314280802474396 22.26126791185899 52-53 22.93700378749437 26.893521459639004 27.92095199124257 22.248522761624052 54-55 22.440445943491046 26.81013308312305 28.096650223141488 22.652770750244414 56-57 22.66655830669484 27.005725018511495 27.873218832963104 22.454497841830563 58-59 22.9484093375686 26.62019672675856 27.915552321119325 22.51584161455352 60-61 22.647846282700883 26.643084603240936 28.52182430399625 22.187244810061937 62-63 22.545851687672204 26.800651384155216 28.3621170057276 22.291379922444982 64-65 22.379648213165442 26.86985851886337 28.271126008973628 22.479367258997556 66-67 22.427659236358906 27.002824287427224 27.91902023952269 22.65049623669118 68-69 22.467734391788543 26.801104443597712 28.187654285317006 22.54350687929674 70-71 22.593905001175628 26.812591625977117 27.969404011732458 22.624099361114794 72-73 22.56998130942384 26.751722332078266 28.052803134960406 22.625493223537486 74-75 22.39081172178001 27.14502838559331 27.73574778827865 22.728412104348028 76-77 22.52261688021586 26.925653676504847 27.786664374490535 22.765065068788758 78-79 22.57295781505254 26.952070095268915 27.81594465828412 22.659027431394424 80-81 22.697371272381602 27.11633809979226 27.54645347140558 22.639837156420562 82-83 22.575250896516902 27.06074283499791 27.709514437994333 22.654491830490855 84-85 22.4906132756237 27.079128750175162 27.59603063272084 22.834227341480293 86-87 22.48052440543908 27.06748289000849 27.52250934503494 22.929483359517487 88-89 22.393399590724925 26.992254400646054 27.407524853745198 23.206821154883816 90-91 22.420660074946948 26.843396993092238 27.63992956792632 23.096013364034494 92-93 22.412325388464055 26.6836873449006 27.922423846670874 22.981563419964466 94-95 22.149665259227717 26.74386814207585 27.8815931038742 23.224873494822234 96-97 22.163618312301296 26.646564610630108 27.88490326923862 23.304913807829976 98-99 22.092132112609928 26.716145692254905 27.570941004388104 23.62078119074706 100-101 21.746349056723027 26.75591094352979 27.71491672400187 23.78282327574531 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 183.0 1 153.5 2 203.5 3 265.0 4 206.5 5 189.0 6 312.0 7 619.5 8 1050.5 9 1334.5 10 1305.5 11 1237.5 12 1296.0 13 1434.5 14 1651.5 15 1857.0 16 2031.0 17 2208.5 18 2409.5 19 2616.5 20 2828.0 21 3169.5 22 3625.0 23 4238.0 24 5052.0 25 6028.5 26 7362.0 27 9117.5 28 10982.0 29 13177.5 30 15953.5 31 18885.5 32 21880.5 33 24288.0 34 26613.5 35 28828.5 36 31310.0 37 33980.5 38 36001.5 39 37374.0 40 38928.0 41 41323.0 42 43486.5 43 45519.5 44 47459.5 45 49335.5 46 50627.5 47 53389.5 48 59944.0 49 59888.0 50 53838.0 51 50496.0 52 47659.0 53 46524.0 54 45279.5 55 41924.0 56 38208.5 57 34627.5 58 31489.5 59 27908.0 60 23523.0 61 19515.5 62 16265.5 63 12750.0 64 9310.0 65 7131.5 66 5564.5 67 4499.5 68 3622.5 69 2546.5 70 1844.5 71 1460.5 72 1188.0 73 1006.5 74 832.0 75 723.5 76 558.5 77 375.0 78 241.0 79 155.5 80 103.0 81 69.0 82 54.5 83 31.5 84 14.5 85 8.5 86 7.5 87 6.0 88 3.0 89 1.0 90 1.5 91 1.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011412564945078836 2 0.009679010776206101 3 0.006356365285866693 4 2.8892569481212243E-4 5 0.005345125354024266 6 0.0 7 6.500828133272756E-4 8 0.0020224798636848573 9 1.4446284740606122E-4 10-11 0.0 12-13 0.0 14-15 6.861985251787908E-4 16-17 0.004370001134033352 18-19 0.0020585955755363723 20-21 6.500828133272756E-4 22-23 5.056199659212143E-4 24-25 3.6115711851515307E-4 26-27 1.4446284740606122E-4 28-29 7.223142370303061E-5 30-31 5.056199659212143E-4 32-33 0.003430992625893954 34-35 0.0011195870673969745 36-37 0.0013362813385060664 38-39 0.005886861031796995 40-41 1.4446284740606122E-4 42-43 0.0022030584229424336 44-45 0.005200662506618205 46-47 0.0 48-49 0.0026364469651606174 50-51 0.001516859897763643 52-53 0.0010473556436939439 54-55 2.5280998296060717E-4 56-57 0.01184595348729702 58-59 0.002275289846645464 60-61 0.003864381168112138 62-63 1.8057855925757653E-4 64-65 0.0035754554733000155 66-67 0.0013723970503575816 68-69 0.002275289846645464 70-71 0.005381241065875781 72-73 0.0037199183207060763 74-75 0.007945456607333368 76-77 0.001408512762209097 78-79 0.0068258695399363925 80-81 0.0031059512192303167 82-83 0.003864381168112138 84-85 0.002672562677012133 86-87 0.0010112399318424287 88-89 0.0028892569481212245 90-91 0.009137275098433373 92-93 0.0032142983547848625 94-95 6.861985251787908E-4 96-97 2.5280998296060717E-4 98-99 0.0010112399318424287 100-101 0.0026364469651606174 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1384439.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.03964862981739 #Duplication Level Percentage of deduplicated Percentage of total 1 82.02733657297419 52.53001812173037 2 11.103870390948241 14.22175916534717 3 3.0691053666994494 5.896332878739589 4 1.260797784233642 3.2296418838229908 5 0.6625848184787502 2.1215849481415248 6 0.4150017547348043 1.5945939932384714 7 0.2618867945606154 1.173979681311569 8 0.1894926824001924 0.9708035839063923 9 0.1466958289409654 0.8454914406755298 >10 0.7754372128179376 9.072931624335395 >50 0.05539963807508699 2.4370435636823657 >100 0.02979539202814456 3.5756112502132567 >500 0.0016928889834912952 0.7400926936546733 >1k 6.77155593396518E-4 0.7812169849329457 >5k 2.2571853113217266E-4 0.8088981862678812 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5901 0.42623763127158365 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5291 0.382176462812735 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3012 0.21756104819352823 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2712 0.195891621082619 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1608 0.11614812931447323 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1443 0.10422994440347318 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 7.223142370303061E-5 0.0 0.0 0.0 0.0 7 7.223142370303061E-5 0.0 0.0 0.0 0.0 8 7.223142370303061E-5 0.0 0.0 0.0 0.0 9 7.223142370303061E-5 0.0 0.0 0.0 7.223142370303061E-5 10-11 7.223142370303061E-5 0.0 0.0 0.0 7.223142370303061E-5 12-13 7.223142370303061E-5 0.0 0.0 0.0 7.223142370303061E-5 14-15 1.4446284740606122E-4 0.0 0.0 0.0 1.4446284740606122E-4 16-17 1.8057855925757653E-4 0.0 0.0 3.6115711851515304E-5 1.4446284740606122E-4 18-19 2.1669427110909185E-4 0.0 0.0 7.223142370303061E-5 1.4446284740606122E-4 20-21 2.1669427110909185E-4 0.0 0.0 1.0834713555454591E-4 1.4446284740606122E-4 22-23 2.1669427110909185E-4 0.0 0.0 3.6115711851515307E-4 2.1669427110909182E-4 24-25 2.1669427110909185E-4 0.0 0.0 5.056199659212143E-4 2.8892569481212243E-4 26-27 2.1669427110909185E-4 0.0 0.0 6.139671014757602E-4 2.8892569481212243E-4 28-29 3.2504140666363775E-4 3.6115711851515304E-5 0.0 0.0011918184911000052 2.8892569481212243E-4 30-31 3.6115711851515307E-4 7.223142370303061E-5 0.0 0.0025280998296060716 2.8892569481212243E-4 32-33 3.6115711851515307E-4 7.223142370303061E-5 0.0 0.004117191151072745 3.6115711851515307E-4 34-35 3.6115711851515307E-4 7.223142370303061E-5 0.0 0.005381241065875781 3.6115711851515307E-4 36-37 3.6115711851515307E-4 7.223142370303061E-5 0.0 0.008414960861403066 3.6115711851515307E-4 38-39 4.333885422181837E-4 7.223142370303061E-5 0.0 0.012604383436178842 4.333885422181837E-4 40-41 4.333885422181837E-4 7.223142370303061E-5 0.0 0.017191078841321285 4.333885422181837E-4 42-43 4.69504254069699E-4 7.223142370303061E-5 0.0 0.024739262618287988 4.333885422181837E-4 44-45 5.778513896242449E-4 7.223142370303061E-5 0.0 0.03207075212414559 4.333885422181837E-4 46-47 5.778513896242449E-4 7.223142370303061E-5 0.0 0.045289102661800196 4.333885422181837E-4 48-49 5.778513896242449E-4 7.223142370303061E-5 0.0 0.06128836301202147 4.333885422181837E-4 50-51 5.778513896242449E-4 7.223142370303061E-5 0.0 0.06887266250083969 4.333885422181837E-4 52-53 5.778513896242449E-4 7.223142370303061E-5 0.0 0.08079084741183974 5.056199659212142E-4 54-55 5.778513896242449E-4 7.223142370303061E-5 0.0 0.10534953147087014 5.778513896242449E-4 56-57 5.778513896242449E-4 7.223142370303061E-5 0.0 0.14103185478016728 6.139671014757602E-4 58-59 5.778513896242449E-4 7.223142370303061E-5 0.0 0.179278393630922 7.223142370303061E-4 60-61 5.778513896242449E-4 7.223142370303061E-5 0.0 0.22778179464750703 7.223142370303061E-4 62-63 5.778513896242449E-4 7.223142370303061E-5 0.0 0.2729986658856042 8.667770844363674E-4 64-65 5.778513896242449E-4 7.223142370303061E-5 0.0 0.31648198295482866 9.028927962878826E-4 66-67 5.778513896242449E-4 7.223142370303061E-5 0.0 0.386943736777135 9.390085081393979E-4 68-69 5.778513896242449E-4 7.223142370303061E-5 0.0 0.48792326711397177 9.390085081393979E-4 70-71 6.500828133272755E-4 7.223142370303061E-5 0.0 0.538051875163875 9.390085081393979E-4 72-73 6.500828133272755E-4 7.223142370303061E-5 0.0 0.6086580918335875 0.0010112399318424287 74-75 6.500828133272755E-4 7.223142370303061E-5 0.0 0.683128689671412 0.0010112399318424287 76-77 6.500828133272755E-4 7.223142370303061E-5 0.0 0.7590800316951487 0.0010112399318424287 78-79 7.223142370303061E-4 7.223142370303061E-5 0.0 0.8427962517669612 0.0010112399318424287 80-81 7.223142370303061E-4 7.223142370303061E-5 0.0 0.9777245512442224 0.0010112399318424287 82-83 7.223142370303061E-4 7.223142370303061E-5 0.0 1.1367781462382958 0.0010112399318424287 84-85 7.223142370303061E-4 7.223142370303061E-5 0.0 1.3594315098028877 0.0010112399318424287 86-87 7.223142370303061E-4 7.223142370303061E-5 0.0 1.6708572930985042 0.0010473556436939439 88-89 7.223142370303061E-4 7.223142370303061E-5 0.0 2.008033578944251 0.0010834713555454593 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 105 6.908216E-5 27.142063 2 GTATCAA 10885 0.0 23.82825 1 GTACATG 6330 0.0 22.963902 1 CCGTATG 125 2.2577781E-4 22.797686 9 GGTATCA 8295 0.0 22.563568 1 GAGTACT 6660 0.0 22.252403 12-13 TACATGG 6520 0.0 22.146591 2 ACATGGG 6545 0.0 20.973412 3 GTACTTT 7065 0.0 20.843075 14-15 AGTACTT 6960 0.0 20.542524 12-13 TATCAAC 12855 0.0 20.211388 2 ATCAACG 12930 0.0 20.130888 3 TCAACGC 12955 0.0 20.092041 4 CAACGCA 13050 0.0 19.982174 5 AACGCAG 13230 0.0 19.781399 6 GTAAGGT 610 0.0 19.466644 4 TAGAACG 180 1.2338287E-4 18.471682 4 AGAGTAC 11200 0.0 18.405712 10-11 GTACCTG 1230 0.0 18.151842 1 TACTTTT 7890 0.0 18.091717 14-15 >>END_MODULE