FastQCFastQC Report
Thu 26 May 2016
SRR936430_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936430_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1547345
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48920.3161544451948337No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40990.26490537016631716No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25300.16350587619438456No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22140.14308379837722035No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16410.10605262562647631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG71550.025.966411
TACATGG73550.024.4188862
ACATGGG73100.023.5934773
GTACCTG14000.022.7400571
GTATCAA104800.022.7154451
GGTATCA79700.022.5360741
GAGTACT51350.022.43783812-13
TACCTGG13800.021.690932
CATGGGA36600.021.678944
CCCTATA3001.6734703E-1020.5891692
GTACTTT55450.020.56289714-15
CGACGGT704.851362E-420.35896518-19
ATCAACG119600.019.743673
TCAACGC119750.019.7189394
CAACGCA120650.019.5737425
TATCAAC120950.019.4453622
AACGCAG122350.019.4189116
AGTACTT54100.019.18950812-13
GTGTAGC8200.019.1225091
CATGGGG34900.018.2424034
Produced by FastQC (version 0.11.5)