##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936430_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1547345 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.331891724211474 31.0 31.0 34.0 30.0 34.0 2 31.378604642145092 31.0 31.0 34.0 28.0 34.0 3 31.4205086777674 31.0 31.0 34.0 28.0 34.0 4 35.06459322258449 37.0 35.0 37.0 32.0 37.0 5 34.98585447977019 37.0 35.0 37.0 32.0 37.0 6 35.0970591561675 37.0 35.0 37.0 32.0 37.0 7 34.928322384471464 37.0 35.0 37.0 32.0 37.0 8 34.91046922308858 37.0 35.0 37.0 32.0 37.0 9 36.41131421887168 39.0 37.0 39.0 32.0 39.0 10-11 36.28655503459151 39.0 35.0 39.0 32.0 39.0 12-13 36.18849060810614 39.0 35.0 39.0 31.5 39.0 14-15 37.26976369200146 40.0 37.0 41.0 32.0 41.0 16-17 37.2019746727459 39.0 36.5 41.0 31.0 41.0 18-19 37.16496902759242 39.5 36.5 41.0 31.0 41.0 20-21 37.072486097153515 39.0 36.0 41.0 31.0 41.0 22-23 36.969693895026644 39.0 36.0 41.0 31.0 41.0 24-25 36.82535762871241 39.0 36.0 41.0 30.0 41.0 26-27 36.623292478406555 39.0 36.0 40.5 30.0 41.0 28-29 36.728516264957065 39.0 36.0 41.0 30.0 41.0 30-31 36.6640057647131 39.0 36.0 41.0 30.0 41.0 32-33 36.522917642801055 39.0 35.5 41.0 30.0 41.0 34-35 36.35580203509883 39.0 35.0 40.5 30.0 41.0 36-37 36.11831750514591 39.0 35.0 40.0 29.5 41.0 38-39 35.95053688737806 38.0 35.0 40.0 29.0 41.0 40-41 35.79498204989837 38.0 35.0 40.0 28.5 41.0 42-43 35.540327787274336 38.0 35.0 40.0 27.0 41.0 44-45 35.26862076653882 38.0 34.0 40.0 26.5 41.0 46-47 34.92815176964413 38.0 34.0 40.0 25.5 41.0 48-49 34.68372308696509 38.0 34.0 40.0 24.0 41.0 50-51 33.98351175723578 37.5 33.0 39.5 23.5 40.5 52-53 34.16793249081491 38.0 33.5 39.5 24.0 40.5 54-55 34.527495807334496 38.0 34.0 40.0 24.0 41.0 56-57 34.45968966196938 38.0 34.0 40.0 24.0 41.0 58-59 34.2272505485202 38.0 34.0 40.0 23.0 41.0 60-61 33.903855313456276 37.5 33.0 40.0 22.5 41.0 62-63 33.57958632367055 37.0 33.0 40.0 22.0 41.0 64-65 33.265240460272274 36.5 32.5 39.5 22.0 41.0 66-67 32.903409388339384 36.0 32.0 39.0 20.5 41.0 68-69 32.53431684595226 35.5 32.0 39.0 19.5 40.5 70-71 32.13311801828293 35.0 31.0 38.5 18.5 40.0 72-73 31.7168288261506 35.0 31.0 37.5 17.5 40.0 74-75 31.30305911092872 35.0 31.0 37.0 14.5 39.0 76-77 30.886072272182354 35.0 30.5 36.5 12.0 39.0 78-79 30.437212774138928 34.5 30.0 36.0 7.5 38.5 80-81 30.029065592999622 34.0 30.0 35.5 7.0 37.0 82-83 29.626782327147467 34.0 29.0 35.0 3.5 37.0 84-85 29.252456304185557 34.0 29.0 35.0 2.0 36.5 86-87 28.910738070695288 34.0 29.0 35.0 2.0 36.0 88-89 28.5377236492185 34.0 29.0 35.0 2.0 36.0 90-91 28.19265839227839 34.0 28.0 35.0 2.0 35.0 92-93 27.834441898865478 34.0 27.0 35.0 2.0 35.0 94-95 27.41991604974973 33.5 26.5 35.0 2.0 35.0 96-97 26.840856434731748 33.0 25.5 35.0 2.0 35.0 98-99 26.185237616691815 33.0 24.5 35.0 2.0 35.0 100-101 24.117443750424115 31.0 12.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2089544697542962 1101 2 0.4247625372955035 1101 3 0.3415477162050351 1101 4 0.3119292707482231 1101 5 0.37146012623622937 1101 6 0.3359193703550005 1101 7 0.17612212485636292 1101 8 0.2744836673316513 1101 9 0.3137748381582455 1101 10-11 0.16997373766236024 1101 12-13 0.2631291346860749 1101 14-15 0.5867416691897631 1101 16-17 0.5549974950957122 1101 18-19 0.612200428917653 1101 20-21 0.674472640203426 1101 22-23 0.4876646260312114 1101 24-25 0.443219962906646 1101 26-27 0.4623996024593211 1101 28-29 0.47168241885939466 1101 30-31 0.29610001838654654 1101 32-33 0.36834833115731413 1101 34-35 0.32586622571891155 1101 36-37 0.4227037481877147 1101 38-39 0.4272669006959049 1101 40-41 0.28732609787541463 1101 42-43 0.2776935940676353 1101 44-45 0.19228946207397257 1101 46-47 0.29311222802785863 1101 48-49 0.0909594902942743 1101 50-51 0.20425268688387632 1101 52-53 0.2641561187903534 1101 54-55 0.2310007892688546 1101 56-57 0.16663136029441716 1101 58-59 0.2857407260044411 1101 60-61 0.058110376234239425 1101 62-63 0.12693223694255806 1101 64-65 0.31449245966891226 1101 66-67 0.2725937184394027 1101 68-69 0.2663624227610768 1101 70-71 0.009091132472910601 1101 72-73 0.06082495361749807 1101 74-75 -0.045303301186223166 1101 76-77 -0.060727747888062567 1101 78-79 -0.06576189895810458 1101 80-81 -0.07827731135486005 1101 82-83 0.06710358883684009 1101 84-85 0.11779953160176504 1101 86-87 0.05373415243929358 1101 88-89 -0.0415263355842157 1101 90-91 0.13710493552356695 1101 92-93 -0.19746634585336764 1101 94-95 -0.19326444479657567 1101 96-97 -0.2969030035945721 1101 98-99 -0.5023431305715356 1101 100-101 -0.019670554094606274 1102 1 0.11305114832843799 1102 2 0.09090096804212777 1102 3 0.08315737899543763 1102 4 0.157148545658913 1102 5 0.08081129544906673 1102 6 0.2131849577482754 1102 7 0.019182219609469087 1102 8 0.09677091619791156 1102 9 0.33413028899556707 1102 10-11 0.26019282256013554 1102 12-13 0.35745028185741745 1102 14-15 0.3602681189883441 1102 16-17 0.17403805652055837 1102 18-19 0.1551870520080243 1102 20-21 -0.07027761948089761 1102 22-23 -0.20691082176590214 1102 24-25 0.011272457142929682 1102 26-27 0.09250619970793394 1102 28-29 -0.08001724671786548 1102 30-31 0.11027498228408916 1102 32-33 -0.19651099594359067 1102 34-35 -0.15113429364973996 1102 36-37 -0.23061957779844988 1102 38-39 -0.29485187004716806 1102 40-41 -0.4870759948002288 1102 42-43 0.03493213628849645 1102 44-45 -0.09564693426764848 1102 46-47 -0.07715264998385152 1102 48-49 0.013042158582386776 1102 50-51 -0.11950747880739243 1102 52-53 -0.3133428678073997 1102 54-55 -0.25010147568516317 1102 56-57 -0.09871091631372764 1102 58-59 -0.1762775912411314 1102 60-61 -0.4305936723077295 1102 62-63 -0.239731262842092 1102 64-65 -0.09054541623998347 1102 66-67 -0.16808899798531485 1102 68-69 -0.1991974186414751 1102 70-71 -0.10035440982396437 1102 72-73 -0.5826788213058656 1102 74-75 -0.6161651364576883 1102 76-77 -0.43240347183729355 1102 78-79 -0.4405636521439895 1102 80-81 -0.2551435549260894 1102 82-83 -0.17001638532140717 1102 84-85 -0.4992243593561554 1102 86-87 -0.6886178274953423 1102 88-89 -0.8497561118756707 1102 90-91 -0.9193032502330638 1102 92-93 -0.652921616811792 1102 94-95 -0.5651355653661092 1102 96-97 -0.9573901333860633 1102 98-99 -1.0926413875196737 1102 100-101 -0.9071398353390627 1103 1 0.20296833395887504 1103 2 0.2780947323116685 1103 3 0.332426119004797 1103 4 0.2950321156271656 1103 5 0.4693608034934016 1103 6 0.19129928994161816 1103 7 0.2076378274016264 1103 8 0.027889271341848598 1103 9 0.39785181664903035 1103 10-11 0.3878004388441383 1103 12-13 0.4652562506473572 1103 14-15 0.5592411831200224 1103 16-17 0.5864424682621703 1103 18-19 0.6400812022421505 1103 20-21 0.3167588069079059 1103 22-23 0.2847534110313532 1103 24-25 0.4536704623121537 1103 26-27 0.4213517918919152 1103 28-29 0.4746454189815381 1103 30-31 0.36223416864939395 1103 32-33 0.44668610351215676 1103 34-35 0.35095412333018317 1103 36-37 0.4918387559274464 1103 38-39 0.37851971429190456 1103 40-41 0.2129147407936216 1103 42-43 0.504419422068743 1103 44-45 0.5553765470763494 1103 46-47 0.7492796429095634 1103 48-49 0.6105022980823591 1103 50-51 0.1082998717922834 1103 52-53 0.47719503303518707 1103 54-55 0.6880906461150857 1103 56-57 0.5679788328564044 1103 58-59 0.5879954041140962 1103 60-61 0.8152211714692541 1103 62-63 0.5856758214451574 1103 64-65 0.6859986776225853 1103 66-67 0.6543782860990675 1103 68-69 0.5244269740688452 1103 70-71 0.5550007309793088 1103 72-73 0.4258700272383713 1103 74-75 0.34306252611693466 1103 76-77 0.3258953947646823 1103 78-79 0.5373702596651491 1103 80-81 0.6077108871211259 1103 82-83 0.6257486819004967 1103 84-85 0.35167884173170805 1103 86-87 0.35028561226874544 1103 88-89 0.5569013845991435 1103 90-91 0.5578997156330949 1103 92-93 0.36952870869861343 1103 94-95 0.39065830002678226 1103 96-97 0.6955901789657446 1103 98-99 0.46604878247432424 1103 100-101 0.5777172572704501 1104 1 0.30532692023017205 1104 2 0.37895382084945695 1104 3 0.3746370112743058 1104 4 0.32098498491576777 1104 5 0.29173650380016625 1104 6 0.3275028388357768 1104 7 0.40897041046823546 1104 8 0.47634256086888627 1104 9 0.5130024297932181 1104 10-11 0.5611364422321401 1104 12-13 0.4944737744502561 1104 14-15 0.6056584989942166 1104 16-17 0.2905237680091588 1104 18-19 0.4723005800655571 1104 20-21 0.5094778225960752 1104 22-23 0.4856081185251995 1104 24-25 0.41178721620094194 1104 26-27 0.48348307344669905 1104 28-29 0.5494410246391155 1104 30-31 0.5011447987709019 1104 32-33 0.4978302861538495 1104 34-35 0.5459394747971089 1104 36-37 0.6608021046110011 1104 38-39 0.7692277375990599 1104 40-41 0.3165107389430162 1104 42-43 0.5621876810014825 1104 44-45 0.39644330331102395 1104 46-47 0.5012629974282987 1104 48-49 0.416962380402488 1104 50-51 0.3876663044822024 1104 52-53 0.5737810501722151 1104 54-55 0.8127605530971351 1104 56-57 0.797062532856188 1104 58-59 0.7079982564533509 1104 60-61 0.7035440115291536 1104 62-63 0.5528274686399968 1104 64-65 0.47526005536457916 1104 66-67 0.2698604293057585 1104 68-69 0.2443311441701823 1104 70-71 0.4855381918567403 1104 72-73 0.2878076417941209 1104 74-75 0.23263039050715761 1104 76-77 0.3413656066176749 1104 78-79 0.4879990016126463 1104 80-81 0.5369812630923718 1104 82-83 0.33345397497835805 1104 84-85 0.4783825163090043 1104 86-87 0.27489980852895 1104 88-89 0.28324941783666446 1104 90-91 0.29128973325359553 1104 92-93 0.26080160703260447 1104 94-95 0.24906404745928867 1104 96-97 0.4888237032110929 1104 98-99 0.5320591521878271 1104 100-101 0.5856398004136345 1105 1 0.042123319223581035 1105 2 0.14489726992867702 1105 3 0.17420435529097844 1105 4 0.23850736200623857 1105 5 0.31407348656526324 1105 6 0.40831876600397976 1105 7 0.394967588026816 1105 8 0.13986803814271553 1105 9 0.35463911607831733 1105 10-11 0.3431340725481249 1105 12-13 0.23245564956974363 1105 14-15 0.2965900663623273 1105 16-17 0.3203421862942193 1105 18-19 0.12120751020236042 1105 20-21 -0.0867649885327424 1105 22-23 -0.25756952819530454 1105 24-25 -0.20670665524576037 1105 26-27 -0.14753629334608576 1105 28-29 0.015492621861312728 1105 30-31 -0.14029559752498244 1105 32-33 -0.3334108937167102 1105 34-35 -0.18927068802117475 1105 36-37 -0.3111722759150979 1105 38-39 -0.3783061771956753 1105 40-41 -0.6666089878045725 1105 42-43 -0.4636743414987805 1105 44-45 -0.29067771449268065 1105 46-47 -0.3499979541168301 1105 48-49 -0.11018657646412322 1105 50-51 -0.22957217976973965 1105 52-53 -0.10496458989770474 1105 54-55 -0.13900857740892292 1105 56-57 0.005976939197076092 1105 58-59 -0.19336229854859255 1105 60-61 0.07015844833922102 1105 62-63 -0.037555336471086775 1105 64-65 -0.10886293485072684 1105 66-67 -0.37247866505896354 1105 68-69 -0.14405242631650594 1105 70-71 -0.027396624077404397 1105 72-73 -0.021422648436686842 1105 74-75 0.05189569334256916 1105 76-77 -0.0650096804378073 1105 78-79 -0.20407650741443817 1105 80-81 -0.020608494951918743 1105 82-83 -0.08933630418082217 1105 84-85 -0.3002086535901114 1105 86-87 -0.1378112285740123 1105 88-89 -0.10539477350469895 1105 90-91 -0.04980018823358279 1105 92-93 -0.016657352316567398 1105 94-95 -0.020018741513595728 1105 96-97 -0.19031952633785565 1105 98-99 -0.259003296310226 1105 100-101 -0.12119122437482943 1106 1 -0.047887649112091424 1106 2 0.018841833246703743 1106 3 -0.0029641794799530885 1106 4 0.18779867021320484 1106 5 0.1789128601114669 1106 6 0.21439793744511348 1106 7 0.2794404458912396 1106 8 0.29959577495497314 1106 9 0.32420827789150763 1106 10-11 0.3032055710581574 1106 12-13 0.3719588215601135 1106 14-15 0.2246358227933598 1106 16-17 0.14214808761843045 1106 18-19 0.29638033096079397 1106 20-21 0.3989810789674735 1106 22-23 0.3547280847613976 1106 24-25 -0.008315943840479179 1106 26-27 0.019322005891687866 1106 28-29 -0.12271494612684108 1106 30-31 0.08417719495443521 1106 32-33 0.02206387502467777 1106 34-35 -0.19993678147923788 1106 36-37 0.16962708854258324 1106 38-39 0.15001860977178438 1106 40-41 -0.07156206588572189 1106 42-43 -0.14501950733968982 1106 44-45 -0.2974891980622445 1106 46-47 -0.3706001173799365 1106 48-49 -0.40526406587584773 1106 50-51 -0.15738663672269837 1106 52-53 -0.39728838955148404 1106 54-55 -0.17155167839116103 1106 56-57 -0.16250143716232657 1106 58-59 -0.18786358254675406 1106 60-61 -0.11755047720523493 1106 62-63 -0.013114092259705501 1106 64-65 -0.264590907049012 1106 66-67 -0.06243765231052123 1106 68-69 -0.1288209878952884 1106 70-71 -0.01786283555541246 1106 72-73 0.06024519243581139 1106 74-75 -0.1424151568096974 1106 76-77 -0.4139157074647031 1106 78-79 -0.22694164592441624 1106 80-81 -0.2534567804751866 1106 82-83 -0.043679258373718 1106 84-85 -0.27310133553616467 1106 86-87 -0.1538281044454699 1106 88-89 -0.0834476405557183 1106 90-91 -0.09932083436826744 1106 92-93 -0.24734842284901148 1106 94-95 -0.2967178119021945 1106 96-97 -0.2102850030518013 1106 98-99 -0.2897481460146025 1106 100-101 0.1338613206550079 1107 1 0.46332494233104526 1107 2 0.5297884082854978 1107 3 0.5824359535282468 1107 4 0.6228330306654328 1107 5 0.3863480186129138 1107 6 0.5298690081611426 1107 7 0.7284694860153849 1107 8 0.6949803903395875 1107 9 0.8774071251537592 1107 10-11 0.9006817404219376 1107 12-13 0.8786258576940043 1107 14-15 0.9044141424564032 1107 16-17 1.0686056358338973 1107 18-19 1.2698806634812954 1107 20-21 1.1962499772161905 1107 22-23 1.1580000865348339 1107 24-25 1.1654293454523597 1107 26-27 1.2441424448187561 1107 28-29 1.2875577206876159 1107 30-31 1.0999685937573602 1107 32-33 1.2823764442997785 1107 34-35 1.060382438205977 1107 36-37 1.1123725995157656 1107 38-39 1.304700498488252 1107 40-41 1.2038214410972117 1107 42-43 1.3281394374619069 1107 44-45 1.040841549000497 1107 46-47 1.2686882887384385 1107 48-49 1.078639724857922 1107 50-51 0.942721986642411 1107 52-53 1.0025719518362308 1107 54-55 1.2977212101036244 1107 56-57 1.3141356721260777 1107 58-59 1.3421774283155798 1107 60-61 1.0927266232159596 1107 62-63 1.4630153690570822 1107 64-65 1.2440612765023076 1107 66-67 1.2261613279599288 1107 68-69 1.2374170967866007 1107 70-71 1.5287720569296184 1107 72-73 1.4144615524446813 1107 74-75 1.2570638943984598 1107 76-77 1.4282744942644037 1107 78-79 1.5045037098725267 1107 80-81 1.3735483181725634 1107 82-83 1.4207332887333557 1107 84-85 1.5659299213913478 1107 86-87 1.326421285933602 1107 88-89 1.564776699945277 1107 90-91 1.5175024864031812 1107 92-93 1.4546533505936843 1107 94-95 1.4091777766590958 1107 96-97 1.501112691472695 1107 98-99 1.6614090253442981 1107 100-101 1.6665281351085675 1108 1 0.15298128875087613 1108 2 0.10259189801844926 1108 3 0.06153372988743655 1108 4 0.1111232743083761 1108 5 0.4163341873542663 1108 6 0.3027693167046053 1108 7 0.4488514415425655 1108 8 0.27801901146949604 1108 9 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-2.584914557295111 2316 78-79 -2.8486349083520217 2316 80-81 -2.9162259559301624 2316 82-83 -2.5980369702883372 2316 84-85 -3.000490780758195 2316 86-87 -3.031908293462177 2316 88-89 -3.404998553445509 2316 90-91 -3.446051839601811 2316 92-93 -3.839059130432613 2316 94-95 -3.873705981848463 2316 96-97 -4.108954144937236 2316 98-99 -4.394033857679744 2316 100-101 -4.978581789975898 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3633.0 3 1376.0 4 1537.0 5 1533.0 6 1794.0 7 2047.0 8 2651.0 9 3352.0 10 4113.0 11 5121.0 12 6544.0 13 7644.0 14 8477.0 15 10029.0 16 12046.0 17 14753.0 18 13029.0 19 9446.0 20 9925.0 21 10636.0 22 11663.0 23 13486.0 24 15411.0 25 18005.0 26 21020.0 27 24738.0 28 29459.0 29 35768.0 30 43595.0 31 53117.0 32 66723.0 33 84862.0 34 111484.0 35 151385.0 36 210889.0 37 274820.0 38 217444.0 39 33774.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.57155599795748 18.41756565467226 14.191621798062194 22.819256549308065 2 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22.633609616596384 26.70625111078797 28.1518103824342 22.508328890181442 46-47 22.831618309500513 26.899818135520704 27.711131476256153 22.55743207872263 48-49 22.835801073265717 27.024749351061033 27.58329086780581 22.55615870786744 50-51 22.68455222079215 27.22866867508617 27.603575085817766 22.483204018303912 52-53 22.941237866611 27.281678166572583 27.309758768563814 22.467325198252606 54-55 22.555114679054725 27.09399911393841 27.522165273430144 22.828720933576726 56-57 22.73497236151513 27.41693751498579 27.224408469209894 22.623681654289186 58-59 23.011144139640557 27.065520897321843 27.218721715125326 22.704613247912274 60-61 22.755184308529973 27.017441249888925 27.829319718548795 22.398054723032306 62-63 22.668250044431517 27.255263115376536 27.65705330166578 22.419433538526164 64-65 22.47879675845528 27.316037684023716 27.51238134877369 22.69278420874731 66-67 22.44591302459543 27.392143537821863 27.28059435164297 22.88134908593973 68-69 22.53455857830908 27.26324109956645 27.501861393408085 22.700338928716388 70-71 22.518336491751487 27.304374129065117 27.270118434217434 22.907170944965962 72-73 22.596341831488477 27.188538316022274 27.42159290159422 22.793526950895025 74-75 22.485555297037383 27.690721782741328 27.12534253658032 22.69838038364097 76-77 22.68082913107335 27.358158578023083 27.13599488134843 22.82501740955514 78-79 22.66572880981181 27.43205440431759 27.120893670694578 22.78132311517602 80-81 22.646155735691476 27.66865117842506 26.98822867781096 22.696964408072503 82-83 22.613221704753002 27.566449122779808 27.141217392653143 22.679111779814047 84-85 22.575990548080757 27.525063033262043 27.02793887534829 22.871007543308906 86-87 22.715730053133907 27.47739013703907 26.99862915688301 22.808250652944018 88-89 22.61265302139084 27.380172964826006 26.876860961796112 23.130313051987038 90-91 22.606559867109205 27.350942225468366 27.090266848077256 22.952231059345177 92-93 22.66331029788796 27.403960355721658 27.197187527571597 22.73554181881878 94-95 22.373812271425972 27.369357531870698 27.194076375919913 23.06275382078342 96-97 22.364569619672313 27.31887303516966 27.28878780769497 23.027769537463055 98-99 22.35608280983551 27.365113389693114 26.90792816390276 23.370875636568613 100-101 22.1750564518345 27.333129670308352 27.211578404607312 23.28023547324983 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 172.0 1 135.5 2 150.0 3 203.0 4 181.0 5 207.5 6 341.5 7 581.0 8 886.5 9 1140.0 10 1178.0 11 1148.5 12 1240.0 13 1345.0 14 1458.0 15 1628.0 16 1804.5 17 2001.0 18 2096.5 19 2221.5 20 2526.0 21 2914.0 22 3377.0 23 4042.5 24 4956.5 25 6025.0 26 7643.5 27 9690.0 28 11739.0 29 14158.5 30 17262.5 31 20685.0 32 24358.0 33 27159.5 34 29912.5 35 32468.5 36 35055.5 37 38266.0 38 40388.5 39 41777.0 40 43310.5 41 46165.5 42 48781.0 43 51064.5 44 53449.0 45 56074.5 46 57632.0 47 59754.5 48 66123.0 49 66169.5 50 60256.5 51 56590.5 52 53618.0 53 52276.0 54 51112.0 55 47848.5 56 43805.0 57 39510.5 58 35656.5 59 31778.5 60 26927.5 61 22311.5 62 18594.0 63 14830.0 64 11169.0 65 8695.5 66 6672.0 67 5323.5 68 4179.0 69 2997.5 70 2268.5 71 1777.0 72 1451.5 73 1229.5 74 962.5 75 768.5 76 589.5 77 378.5 78 251.0 79 180.0 80 119.0 81 70.0 82 43.5 83 35.0 84 21.5 85 15.5 86 16.0 87 14.0 88 9.0 89 9.0 90 7.0 91 3.0 92 4.0 93 1.5 94 2.5 95 2.5 96 0.5 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01518730470580252 2 0.0065919365106036475 3 0.008789248680804863 4 0.0341875922951895 5 0.046466689716902175 6 0.06352817245022926 7 0.0292113264979691 8 0.026303119213879254 9 0.016027453476761808 10-11 0.04808236043028542 12-13 0.0472745250735938 14-15 0.022619389987365457 16-17 0.025979985071202607 18-19 0.0329919959672859 20-21 0.024105807043678044 22-23 0.015154991291534855 24-25 0.0036837292265138027 26-27 0.0032313414267664937 28-29 0.0016156707133832468 30-31 0.00817529380971923 32-33 0.0021326853416658857 34-35 0.0018741780275245662 36-37 0.006818130410477301 38-39 0.00759365235290126 40-41 0.0049762657972204 42-43 0.0011309694993682728 44-45 0.0012925365707065974 46-47 0.0025204463128778646 48-49 7.432085281562935E-4 50-51 0.0016479841276509119 52-53 8.724621852269532E-4 54-55 0.0031344011839634987 56-57 0.002972834112625174 58-59 0.0019064914417922312 60-61 4.84701214014974E-4 62-63 0.0012925365707065974 64-65 0.0037483560550491324 66-67 0.0029405206983575093 68-69 0.007561338938633595 70-71 0.010146412080046789 72-73 0.004394624340402432 74-75 0.004200743854796442 76-77 0.004039176783458117 78-79 0.035932516665643406 80-81 0.023588792415395403 82-83 0.004588504826008421 84-85 0.009435516966158162 86-87 0.007884473081310244 88-89 0.0060749218823210075 90-91 0.013571633992419275 92-93 0.015284244948605516 94-95 0.00752902552436593 96-97 0.004782385311614411 98-99 0.02449356801489002 100-101 0.04346154219000934 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1547345.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.53933944086143 #Duplication Level Percentage of deduplicated Percentage of total 1 82.73092813215315 54.22130381110698 2 10.694711759209918 14.018486884180623 3 2.9778838922822617 5.855056296952824 4 1.1674755335034233 3.060623011167266 5 0.6497819346200003 2.1293139387799918 6 0.3819643929617611 1.5020216402786066 7 0.2589920427026294 1.1881917179418793 8 0.17689760391130321 0.9275001687214367 9 0.13470683030224165 0.7945737008563022 >10 0.7454758406490416 8.917310176171293 >50 0.05408199323893581 2.465744602654165 >100 0.024924253135551314 3.140032513842433 >500 0.001582393694380648 0.7057399999499512 >1k 5.933976353927429E-4 1.074101537396275 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4892 0.3161544451948337 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4099 0.26490537016631716 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2530 0.16350587619438456 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2214 0.14308379837722035 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1641 0.10605262562647631 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 6.462682853532988E-5 7 0.0 0.0 0.0 0.0 6.462682853532988E-5 8 0.0 0.0 0.0 0.0 1.2925365707065975E-4 9 0.0 0.0 0.0 0.0 1.2925365707065975E-4 10-11 0.0 0.0 0.0 0.0 1.2925365707065975E-4 12-13 6.462682853532988E-5 0.0 0.0 0.0 1.2925365707065975E-4 14-15 6.462682853532988E-5 0.0 0.0 0.0 1.9388048560598962E-4 16-17 6.462682853532988E-5 0.0 0.0 0.0 2.585073141413195E-4 18-19 6.462682853532988E-5 0.0 0.0 3.231341426766494E-5 2.585073141413195E-4 20-21 6.462682853532988E-5 0.0 0.0 6.462682853532988E-5 2.585073141413195E-4 22-23 6.462682853532988E-5 0.0 0.0 1.2925365707065975E-4 3.2313414267664934E-4 24-25 6.462682853532988E-5 6.462682853532988E-5 0.0 2.585073141413195E-4 3.8776097121197923E-4 26-27 2.585073141413195E-4 6.462682853532988E-5 0.0 3.8776097121197923E-4 3.8776097121197923E-4 28-29 3.554475569443143E-4 6.462682853532988E-5 0.0 7.432085281562935E-4 3.8776097121197923E-4 30-31 3.8776097121197923E-4 6.462682853532988E-5 0.0 0.0018741780275245664 3.8776097121197923E-4 32-33 3.8776097121197923E-4 6.462682853532988E-5 0.0 0.0032636548410341585 4.523877997473091E-4 34-35 3.8776097121197923E-4 6.462682853532988E-5 0.0 0.0051701462828263895 4.523877997473091E-4 36-37 3.8776097121197923E-4 6.462682853532988E-5 0.0 0.007949099909845575 4.523877997473091E-4 38-39 4.523877997473091E-4 6.462682853532988E-5 0.0 0.011891336450500696 5.816414568179688E-4 40-41 4.523877997473091E-4 6.462682853532988E-5 0.0 0.015898199819691147 5.816414568179688E-4 42-43 4.523877997473091E-4 6.462682853532988E-5 0.0 0.022490136330294796 5.816414568179688E-4 44-45 4.523877997473091E-4 6.462682853532988E-5 0.0 0.02788647651299484 6.139548710856337E-4 46-47 5.49328042550304E-4 6.462682853532988E-5 0.0 0.038194455664379956 6.462682853532987E-4 48-49 6.462682853532987E-4 6.462682853532988E-5 0.0 0.05050586650036029 6.462682853532987E-4 50-51 6.462682853532987E-4 6.462682853532988E-5 0.0 0.05706548959669628 6.462682853532987E-4 52-53 6.462682853532987E-4 6.462682853532988E-5 0.0 0.06792279679063169 6.785816996209636E-4 54-55 6.462682853532987E-4 6.462682853532988E-5 0.0 0.08711696486562466 7.108951138886286E-4 56-57 7.108951138886286E-4 6.462682853532988E-5 0.0 0.11577896332104347 7.108951138886286E-4 58-59 7.108951138886286E-4 6.462682853532988E-5 0.0 0.14589506541850716 7.108951138886286E-4 60-61 7.108951138886286E-4 6.462682853532988E-5 0.0 0.18677153446710332 7.108951138886286E-4 62-63 7.108951138886286E-4 6.462682853532988E-5 0.0 0.22448128891746832 7.108951138886286E-4 64-65 7.108951138886286E-4 6.462682853532988E-5 0.0 0.2612216409398034 7.108951138886286E-4 66-67 7.108951138886286E-4 6.462682853532988E-5 0.0 0.3229402621910433 7.108951138886286E-4 68-69 7.108951138886286E-4 6.462682853532988E-5 0.0 0.4019788734897518 7.108951138886286E-4 70-71 7.432085281562935E-4 6.462682853532988E-5 0.0 0.4480254888211743 7.108951138886286E-4 72-73 7.755219424239585E-4 6.462682853532988E-5 0.0 0.5062219479172388 9.047755994946183E-4 74-75 7.755219424239585E-4 6.462682853532988E-5 0.0 0.5682313898968878 9.047755994946183E-4 76-77 7.755219424239585E-4 6.462682853532988E-5 0.0 0.6362511269303226 9.047755994946183E-4 78-79 7.755219424239585E-4 6.462682853532988E-5 0.0 0.7123815309449412 9.047755994946183E-4 80-81 8.401487709592884E-4 6.462682853532988E-5 0.0 0.8312302686214128 9.047755994946183E-4 82-83 8.401487709592884E-4 6.462682853532988E-5 0.0 0.970371830457978 9.69402428029948E-4 84-85 8.724621852269533E-4 6.462682853532988E-5 0.0 1.170391864774824 9.69402428029948E-4 86-87 9.047755994946183E-4 6.462682853532988E-5 0.0 1.462408189511712 9.69402428029948E-4 88-89 9.047755994946183E-4 9.694024280299481E-5 0.0 1.7694825652973318 0.001034029256565278 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7155 0.0 25.96641 1 TACATGG 7355 0.0 24.418886 2 ACATGGG 7310 0.0 23.593477 3 GTACCTG 1400 0.0 22.740057 1 GTATCAA 10480 0.0 22.715445 1 GGTATCA 7970 0.0 22.536074 1 GAGTACT 5135 0.0 22.437838 12-13 TACCTGG 1380 0.0 21.69093 2 CATGGGA 3660 0.0 21.67894 4 CCCTATA 300 1.6734703E-10 20.589169 2 GTACTTT 5545 0.0 20.562897 14-15 CGACGGT 70 4.851362E-4 20.358965 18-19 ATCAACG 11960 0.0 19.74367 3 TCAACGC 11975 0.0 19.718939 4 CAACGCA 12065 0.0 19.573742 5 TATCAAC 12095 0.0 19.445362 2 AACGCAG 12235 0.0 19.418911 6 AGTACTT 5410 0.0 19.189508 12-13 GTGTAGC 820 0.0 19.122509 1 CATGGGG 3490 0.0 18.242403 4 >>END_MODULE