FastQCFastQC Report
Thu 26 May 2016
SRR936430_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936430_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1547345
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61030.3944175345511182No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57580.3721212787064294No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33130.2141086829375479No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28240.18250616378377155No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16890.10915471339617215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81250.025.2977391
GTACATG65000.024.6771161
GTACACG2751.8189894E-1224.448751
GTATCAA108150.024.3341031
TACATGG67650.024.0146922
ACATGGG68550.023.0049483
GAGTACT69500.022.61703112-13
GTACTTT76400.020.41899714-15
AGTACTT72300.020.3289412-13
TATCAAC130600.020.2595942
TAAGGTG6800.020.2526345
ATCAACG130750.020.2350443
GTGTAGC9500.020.220771
TCAACGC131050.020.1874164
CAACGCA132200.020.0085755
AACGCAG134200.019.7457716
CATGGGG31800.019.4168514
GTAAGGT5950.019.1582954
GTACCTG14100.019.0734921
GTATAGG3556.730261E-1118.9391731
Produced by FastQC (version 0.11.5)