##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936430_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1547345 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.510453066381448 31.0 31.0 34.0 30.0 34.0 2 31.734126519942222 31.0 31.0 34.0 30.0 34.0 3 32.01582646404002 33.0 31.0 34.0 30.0 34.0 4 35.82033160025721 37.0 35.0 37.0 35.0 37.0 5 35.63532502447741 37.0 35.0 37.0 33.0 37.0 6 35.7365455021343 37.0 35.0 37.0 33.0 37.0 7 35.67630425018338 37.0 35.0 37.0 33.0 37.0 8 35.65363445126975 37.0 35.0 37.0 33.0 37.0 9 37.2975929737712 39.0 37.0 39.0 34.0 39.0 10-11 37.18223085349421 39.0 37.0 39.0 33.0 39.0 12-13 37.05429784566467 39.0 37.0 39.0 33.0 39.0 14-15 38.19702878155809 40.0 37.5 41.0 33.0 41.0 16-17 38.10304780123373 40.0 37.0 41.0 33.0 41.0 18-19 38.1639989788961 40.0 37.5 41.0 33.5 41.0 20-21 38.243160381169034 40.0 38.0 41.0 33.0 41.0 22-23 38.1620249524185 40.0 37.5 41.0 33.0 41.0 24-25 38.057794803356714 40.0 37.0 41.0 33.0 41.0 26-27 37.85231541769935 40.0 37.0 41.0 33.0 41.0 28-29 37.770690117588515 40.0 37.0 41.0 32.5 41.0 30-31 37.75653070259057 40.0 37.0 41.0 33.0 41.0 32-33 37.581282131651314 40.0 36.5 41.0 32.5 41.0 34-35 37.41357841980941 40.0 36.0 41.0 32.0 41.0 36-37 37.17674274321499 39.0 36.0 41.0 31.5 41.0 38-39 37.01913180318546 39.0 36.0 41.0 31.0 41.0 40-41 36.787204211084145 39.0 35.5 41.0 30.5 41.0 42-43 36.55076986709493 39.0 35.0 41.0 30.0 41.0 44-45 36.26882692612184 39.0 35.0 40.0 30.0 41.0 46-47 35.853499704332265 39.0 35.0 40.0 28.5 41.0 48-49 35.39600864706966 38.0 35.0 40.0 27.0 41.0 50-51 35.18622866910741 38.0 34.5 40.0 27.0 41.0 52-53 34.91489777651397 38.0 34.0 40.0 26.0 41.0 54-55 34.64511114198837 38.0 34.0 40.0 26.0 41.0 56-57 34.36316076893 38.0 33.5 40.0 25.0 41.0 58-59 34.00040391767834 37.5 33.0 40.0 23.5 41.0 60-61 33.626856001731994 37.0 33.0 40.0 23.0 41.0 62-63 33.33546784976848 36.0 32.0 39.0 22.5 40.5 64-65 32.90229909942514 36.0 32.0 39.0 22.0 40.0 66-67 32.5145103386769 35.0 31.0 39.0 21.0 40.0 68-69 32.06133021401174 35.0 31.0 38.0 20.0 40.0 70-71 31.57991236602051 35.0 31.0 37.5 18.5 39.5 72-73 31.165260171454975 34.5 30.0 37.0 18.0 39.0 74-75 30.796796448109504 34.0 30.0 36.5 15.5 39.0 76-77 29.38242150263839 32.5 28.5 35.0 13.0 37.0 78-79 30.17598596305284 34.0 30.0 35.0 12.5 37.5 80-81 30.021624783096208 34.0 30.0 35.0 9.0 37.0 82-83 29.689555981374546 34.0 29.5 35.0 7.0 37.0 84-85 29.24055107296692 34.0 29.0 35.0 4.0 36.0 86-87 28.856110951339232 34.0 29.0 35.0 2.0 36.0 88-89 28.44387967777063 33.0 29.0 35.0 2.0 35.0 90-91 27.99321289046722 33.0 27.0 35.0 2.0 35.0 92-93 27.53340690020648 33.0 26.5 35.0 2.0 35.0 94-95 27.004710972666082 33.0 25.5 34.5 2.0 35.0 96-97 26.318169832842706 32.0 25.0 34.0 2.0 35.0 98-99 25.4591038197687 32.0 22.0 34.0 2.0 35.0 100-101 22.618249000707664 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4083159376838488 1101 2 0.3649978547957815 1101 3 0.29092794733055527 1101 4 0.08567704992090341 1101 5 0.18307229628515387 1101 6 0.16976031175997264 1101 7 0.1856706089923037 1101 8 0.12859172898065907 1101 9 0.19188476873199534 1101 10-11 0.29296860355518106 1101 12-13 0.25365429213460544 1101 14-15 0.32221796264624203 1101 16-17 0.252050184770475 1101 18-19 0.3099347064666489 1101 20-21 0.09422371923897543 1101 22-23 0.09332093402851172 1101 24-25 0.12930256005051177 1101 26-27 0.2305384908988941 1101 28-29 0.2538749494543069 1101 30-31 0.21611469474320444 1101 32-33 0.20708182755898008 1101 34-35 0.30628166282446756 1101 36-37 0.30956521248062785 1101 38-39 0.5300502827170348 1101 40-41 0.5705839131829649 1101 42-43 0.3551794631973806 1101 44-45 0.38523572098641523 1101 46-47 0.5213911533193212 1101 48-49 0.5276148429500367 1101 50-51 0.4620763120253599 1101 52-53 0.3127138391990911 1101 54-55 0.25462139042786447 1101 56-57 0.30113849271260307 1101 58-59 0.3780056338449995 1101 60-61 0.5286781986776745 1101 62-63 0.2066771966086165 1101 64-65 0.18911390727591737 1101 66-67 0.030519291354110578 1101 68-69 -0.06510622296695345 1101 70-71 -0.04710467167308252 1101 72-73 0.050278908911678855 1101 74-75 -0.02151517215651566 1101 76-77 0.4171268130701371 1101 78-79 0.390296157466107 1101 80-81 0.2248805022324838 1101 82-83 0.2617040028676776 1101 84-85 0.34687489248980086 1101 86-87 0.14320865922838877 1101 88-89 -0.04242495966682469 1101 90-91 -0.00601633364736287 1101 92-93 0.1590837361052948 1101 94-95 -0.045281784198376585 1101 96-97 0.0028251707855915242 1101 98-99 -0.05158862941183884 1101 100-101 -0.01950935788624264 1102 1 0.3747362209297762 1102 2 0.4413292119947343 1102 3 0.14576694304892968 1102 4 0.11765179086995659 1102 5 0.13341811980114926 1102 6 0.1813118305965915 1102 7 0.14890247542588497 1102 8 -0.06472931685046035 1102 9 -0.01684252472395542 1102 10-11 0.09098771885502543 1102 12-13 0.1483703494931703 1102 14-15 0.23128411247775915 1102 16-17 0.2750493487136296 1102 18-19 0.19555104504433984 1102 20-21 0.16411553854936045 1102 22-23 0.04663095911021742 1102 24-25 0.14081322610913816 1102 26-27 -0.010406126668442539 1102 28-29 0.057528581234599585 1102 30-31 0.153472501740751 1102 32-33 0.20884894772315477 1102 34-35 0.26626494168743164 1102 36-37 0.19372161844956537 1102 38-39 0.19051904459283975 1102 40-41 0.09545090413835311 1102 42-43 0.009497190136144695 1102 44-45 -0.07568305482367776 1102 46-47 -0.04935160718839171 1102 48-49 -0.04587170413516617 1102 50-51 -0.15740792956973593 1102 52-53 -0.1637080908254802 1102 54-55 -0.014595883013399202 1102 56-57 -0.08497916328800414 1102 58-59 -0.12419281564957174 1102 60-61 -0.032578483443380435 1102 62-63 -0.10520589477493303 1102 64-65 -0.18784702606755133 1102 66-67 -0.19822871351022542 1102 68-69 -0.30413361016261575 1102 70-71 -0.3720597019484728 1102 72-73 -0.5136205362505919 1102 74-75 -0.7997248070783556 1102 76-77 -0.3692815626994168 1102 78-79 -0.18223367456247175 1102 80-81 -0.26182714388745154 1102 82-83 -0.15133373369841152 1102 84-85 -0.10108753562197847 1102 86-87 -0.2362807127155726 1102 88-89 -0.19931134460766486 1102 90-91 -0.08566848332687371 1102 92-93 0.037831740969622274 1102 94-95 -0.27959336738603113 1102 96-97 -0.24383883570018483 1102 98-99 -0.6497255527226251 1102 100-101 -0.9533550927295451 1103 1 0.4619783485399047 1103 2 0.5133105073157438 1103 3 0.24125847475361795 1103 4 0.1448794094779089 1103 5 0.14532586515873902 1103 6 0.11009649753333406 1103 7 0.12409647837165494 1103 8 0.20941733712867716 1103 9 0.10738185605255524 1103 10-11 0.15096878178075457 1103 12-13 0.2217234050199366 1103 14-15 0.27688697408009233 1103 16-17 0.2814860696791328 1103 18-19 0.3460613725018433 1103 20-21 0.2834706102621425 1103 22-23 0.2288970014501217 1103 24-25 0.1465102077970144 1103 26-27 0.30580171635797626 1103 28-29 0.288616316731094 1103 30-31 0.2685902853179414 1103 32-33 0.18711370874857636 1103 34-35 0.1042822959614611 1103 36-37 0.13171431998180338 1103 38-39 0.1778726591244535 1103 40-41 0.02479583211152203 1103 42-43 0.06721648177023098 1103 44-45 0.2747137444025114 1103 46-47 0.2786658412999472 1103 48-49 0.1816713753534387 1103 50-51 -0.22033428853249148 1103 52-53 0.06342861098312369 1103 54-55 0.162694074056283 1103 56-57 -0.005542847443990695 1103 58-59 0.1084260592432642 1103 60-61 0.2133786239263813 1103 62-63 -0.005941080361395734 1103 64-65 0.005472709001836051 1103 66-67 -0.0059787818155925265 1103 68-69 0.005257357198409807 1103 70-71 -0.07148106912358543 1103 72-73 0.027688194588574078 1103 74-75 -0.0389468481997568 1103 76-77 -0.10279924201278234 1103 78-79 0.046103631473837225 1103 80-81 0.029060920102899956 1103 82-83 0.07944607806141946 1103 84-85 0.06467059114650198 1103 86-87 0.12220265689026633 1103 88-89 0.1258762203609436 1103 90-91 -0.051606559408060804 1103 92-93 -0.005502126244742556 1103 94-95 0.02517293404319787 1103 96-97 -0.15240275853239282 1103 98-99 -0.0347723301224967 1103 100-101 0.23635120989306557 1104 1 0.34487987976136836 1104 2 0.40949468018862945 1104 3 0.15260944888339267 1104 4 0.047388442662295915 1104 5 0.09365440368287636 1104 6 0.2393829684035893 1104 7 0.16093204722845655 1104 8 0.01687294177333598 1104 9 0.06313113729724762 1104 10-11 0.12258334561364848 1104 12-13 0.1309705459227004 1104 14-15 0.13495311292935952 1104 16-17 0.11472116612890204 1104 18-19 0.12053501803686117 1104 20-21 0.12486563407979645 1104 22-23 0.04262247032280442 1104 24-25 0.17522329012242466 1104 26-27 0.18896937269521885 1104 28-29 0.09726971743101132 1104 30-31 0.015239641164662032 1104 32-33 0.022356162245039002 1104 34-35 0.09519597976380823 1104 36-37 0.04797517044497113 1104 38-39 0.11937997438033676 1104 40-41 0.27975612152142304 1104 42-43 0.35209666021853536 1104 44-45 0.24282300701688797 1104 46-47 0.3668983572837661 1104 48-49 0.2813222589727147 1104 50-51 0.21880135946273782 1104 52-53 0.10301305187647358 1104 54-55 0.2638672927657595 1104 56-57 0.41531405989319836 1104 58-59 0.3272372987027339 1104 60-61 -0.030148694368271833 1104 62-63 -0.06339924838282229 1104 64-65 0.043246473757172055 1104 66-67 0.17992118559069326 1104 68-69 -0.08744941642372694 1104 70-71 -0.07176521555485138 1104 72-73 -0.18237260870845518 1104 74-75 -0.20650857353564334 1104 76-77 0.0012805352437581519 1104 78-79 0.19750645106400455 1104 80-81 0.14033058081435357 1104 82-83 0.11956090491230853 1104 84-85 0.22284181290780225 1104 86-87 0.24190629324994717 1104 88-89 0.06150159574028535 1104 90-91 0.02025219324969285 1104 92-93 0.0713084489117577 1104 94-95 0.17975045692638503 1104 96-97 0.2603703459592346 1104 98-99 0.30964716757372557 1104 100-101 0.17189983932541608 1105 1 0.26982804886815615 1105 2 0.35459499226783464 1105 3 0.14634345137610083 1105 4 0.04591127409545237 1105 5 -0.08597582541933946 1105 6 -0.028385302243876254 1105 7 -0.03277487859637063 1105 8 -0.06469662988583025 1105 9 -0.0192161751849369 1105 10-11 -0.09430266637047424 1105 12-13 -0.1271051336810629 1105 14-15 -0.13832053548042467 1105 16-17 -0.01166916845183863 1105 18-19 -0.10906054088231798 1105 20-21 -0.056619854062752495 1105 22-23 -0.1210647060214427 1105 24-25 -0.1579573274221815 1105 26-27 0.0011238796446306765 1105 28-29 -0.0897436595209129 1105 30-31 -0.09674124537109918 1105 32-33 -0.04295123878482343 1105 34-35 -0.07927953661900489 1105 36-37 -0.09914989934720353 1105 38-39 -0.04011729369801742 1105 40-41 -0.18748650032437553 1105 42-43 -0.24637268369858134 1105 44-45 -0.32136629249956883 1105 46-47 0.09196856163016776 1105 48-49 0.01932965718076929 1105 50-51 -0.22991498771087038 1105 52-53 -0.13717284498425641 1105 54-55 -0.15733088466377154 1105 56-57 -0.16987249803136706 1105 58-59 -0.2220152869763723 1105 60-61 -0.3470373379254923 1105 62-63 -0.4962720986967284 1105 64-65 -0.4737228925124626 1105 66-67 -0.35664801035593285 1105 68-69 -0.5487237572859982 1105 70-71 -0.4211861267956962 1105 72-73 -0.6087698868375178 1105 74-75 -0.5781780086383606 1105 76-77 -0.5175948191863391 1105 78-79 -0.6280343609887282 1105 80-81 -0.7783576148681561 1105 82-83 -0.47361374561953795 1105 84-85 -0.5381734606791007 1105 86-87 -0.6269525900689565 1105 88-89 -0.572648074821636 1105 90-91 -0.8060597014290423 1105 92-93 -0.5474812367167132 1105 94-95 -0.2539302546256188 1105 96-97 -0.4530131373367752 1105 98-99 -0.5107795150557024 1105 100-101 -0.1690331389463502 1106 1 0.33164369255907644 1106 2 0.290572931364963 1106 3 0.08702453434804269 1106 4 0.04422662927500909 1106 5 -0.1414854116793336 1106 6 -0.047207031644738606 1106 7 -0.03408650192639584 1106 8 0.0011428074107584507 1106 9 0.012596248294165946 1106 10-11 -0.14292279743472847 1106 12-13 -0.0832241082479257 1106 14-15 -7.418078400576178E-4 1106 16-17 0.013421839311000383 1106 18-19 0.034657022022145156 1106 20-21 0.04089796743944163 1106 22-23 0.06637891983898214 1106 24-25 -0.052652151054282115 1106 26-27 -0.06508220410375998 1106 28-29 -0.08898715493824483 1106 30-31 -0.047181204767007046 1106 32-33 0.028088922323846077 1106 34-35 -0.41412386307982274 1106 36-37 -0.10925384516918513 1106 38-39 -0.009226962343120704 1106 40-41 0.01701100768936925 1106 42-43 -0.25044110257458385 1106 44-45 -0.30720912981675497 1106 46-47 -0.3114626803090843 1106 48-49 -0.2488976407345902 1106 50-51 -0.22060661401591375 1106 52-53 -0.10381864293476184 1106 54-55 -0.16457227468373503 1106 56-57 -0.1471317545057076 1106 58-59 -0.23964243221772819 1106 60-61 -0.2792383118352433 1106 62-63 -0.7258102680144773 1106 64-65 -0.4123402610599811 1106 66-67 -0.33999591909806526 1106 68-69 -0.35892447292495433 1106 70-71 -0.5104167250195104 1106 72-73 -0.2878231445639834 1106 74-75 -0.12079971875063933 1106 76-77 -0.15076937591682693 1106 78-79 -0.08236799781748871 1106 80-81 -0.26800357137731723 1106 82-83 -0.10487742538990474 1106 84-85 -0.017751760047644893 1106 86-87 -0.17188451609888844 1106 88-89 -0.24309421310113066 1106 90-91 -0.3299039804857351 1106 92-93 0.07729347105314943 1106 94-95 0.12745005565895084 1106 96-97 -0.20860625294891832 1106 98-99 -0.00948963224199062 1106 100-101 0.08629794623233877 1107 1 0.5824328745586307 1107 2 0.6459619803563186 1107 3 0.43672304908978177 1107 4 0.3337056804831704 1107 5 0.12704263088328815 1107 6 0.3576699357682571 1107 7 0.21113628681224128 1107 8 0.2794558200465431 1107 9 0.36460467214696735 1107 10-11 0.29681561976766346 1107 12-13 0.45317912828499374 1107 14-15 0.4815470416389971 1107 16-17 0.4257273148153189 1107 18-19 0.4073415709026591 1107 20-21 0.4186700800530545 1107 22-23 0.4445644662810082 1107 24-25 0.5420474720891448 1107 26-27 0.4213387668561097 1107 28-29 0.5262921622863175 1107 30-31 0.48878975510884715 1107 32-33 0.6310849320777763 1107 34-35 0.7498242637213295 1107 36-37 0.6730327875065001 1107 38-39 0.7260601136932365 1107 40-41 0.6266308259114979 1107 42-43 0.6017679419831694 1107 44-45 0.40881281653660295 1107 46-47 0.5319456548715848 1107 48-49 0.7806667574032886 1107 50-51 0.5040675158887638 1107 52-53 0.3083105967016877 1107 54-55 0.4854975546224054 1107 56-57 0.44277181454771863 1107 58-59 0.31386455071288566 1107 60-61 0.3700312514544919 1107 62-63 0.4240944094406274 1107 64-65 0.5096692711945039 1107 66-67 0.509593972278573 1107 68-69 0.36011454281435107 1107 70-71 0.3974296505069823 1107 72-73 0.37371975058121976 1107 74-75 0.5739996605444162 1107 76-77 0.35771322217672363 1107 78-79 0.39667766833192175 1107 80-81 0.4348416957474903 1107 82-83 0.6325189390525701 1107 84-85 0.48591594112726 1107 86-87 0.8233759582970315 1107 88-89 0.9686504111506977 1107 90-91 1.0524779747348383 1107 92-93 1.2742398244116089 1107 94-95 1.0143310133180101 1107 96-97 0.8417196171464028 1107 98-99 1.0178103012539097 1107 100-101 1.3797783275225193 1108 1 0.3414614946245962 1108 2 0.33713763734110813 1108 3 0.11052770916000654 1108 4 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-1.5015368855482834 2316 50-51 -1.7256227842630238 2316 52-53 -1.859029901755946 2316 54-55 -1.5563545239164895 2316 56-57 -1.5390778157710443 2316 58-59 -1.649221675600593 2316 60-61 -1.3207299001882618 2316 62-63 -1.3435430581204528 2316 64-65 -1.3048636084020266 2316 66-67 -1.2617324733954263 2316 68-69 -1.6514804726094354 2316 70-71 -1.2119585392790455 2316 72-73 -1.3245155723390134 2316 74-75 -1.5734527215737835 2316 76-77 -1.524803792953275 2316 78-79 -1.3130307486489308 2316 80-81 -1.313204869399648 2316 82-83 -1.2394261718518038 2316 84-85 -1.4460374937255978 2316 86-87 -1.471676678436065 2316 88-89 -1.5135187118968432 2316 90-91 -1.716876429613201 2316 92-93 -1.7824327995907439 2316 94-95 -1.875976972776101 2316 96-97 -1.7109739146559733 2316 98-99 -2.0014204679768604 2316 100-101 -2.875819283014618 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 8.0 4 16.0 5 43.0 6 120.0 7 270.0 8 630.0 9 1210.0 10 2065.0 11 3208.0 12 4715.0 13 6312.0 14 8043.0 15 10422.0 16 13403.0 17 17082.0 18 14848.0 19 10019.0 20 9106.0 21 9308.0 22 9792.0 23 11182.0 24 12882.0 25 15753.0 26 19657.0 27 24293.0 28 30283.0 29 37586.0 30 45789.0 31 56871.0 32 71426.0 33 90942.0 34 119233.0 35 161954.0 36 229470.0 37 298940.0 38 188546.0 39 11916.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.22091431521103 19.08111092597856 14.964242192579233 22.73373256623117 2 15.802652508921305 20.433070331754287 39.472729987415484 24.291547171908924 3 19.789824045499348 24.148260651791052 30.084632660645326 25.977282642064274 4 13.346863719067825 16.80435126863525 36.44614076987464 33.402644242422284 5 13.74011613441088 37.24062830202702 33.98556882918806 15.03368673437404 6 32.302750840956605 35.842362239836625 16.944508173678138 14.910378745528632 7 28.82110970727278 32.74654327250872 20.128801269270912 18.303545750947592 8 26.93600974572574 34.265015235774825 19.264288183953802 19.534686834545624 9 26.456628181728874 16.48695252030671 20.236390240371442 36.820029057592976 10-11 25.45753415450416 26.361502794951473 26.634842231217565 21.5461208193268 12-13 26.437333908943323 23.7544140152416 27.485807939281763 22.322444136533313 14-15 23.025569604710004 25.09543766903955 26.282955643376233 25.59603708287421 16-17 22.198313886043515 28.392310699940865 27.029298572716492 22.38007684129913 18-19 22.010540635734113 26.581240770481052 29.74162193951575 21.66659665426909 20-21 22.741114618911748 25.486850056063776 30.024170433872214 21.747864891152265 22-23 22.37126041068189 25.621958872353584 29.93597353891771 22.070807178046813 24-25 22.423053682275125 25.731527228898532 30.06753503581942 21.777884053006925 26-27 22.242816644903577 25.84398626162435 29.966629926302772 21.946567167169302 28-29 21.843609537627355 26.208796357631943 30.05994138346652 21.887652721274183 30-31 22.044663601200767 25.74047804465068 30.007884473081308 22.206973881067245 32-33 21.920227391777406 25.92514438996552 29.944150578545457 22.210477639711613 34-35 22.52760799634209 25.932790786724897 29.588695383829915 21.9509058331031 36-37 22.490649859788288 25.712610910726603 29.660997231957797 22.13574199752731 38-39 22.27588767458551 25.95722927714499 29.7838198176423 21.983063230627202 40-41 22.407305676376115 25.92506899159175 29.601173664923834 22.0664516671083 42-43 22.17792313136438 26.08576220684663 29.267637677459383 22.468676984329605 44-45 22.5244893224163 26.10247998619537 29.02626951212655 22.346761179261776 46-47 22.721629509756276 26.468223603255193 28.492637554675465 22.31750933231307 48-49 22.939430995725164 26.560470115576972 28.167573038675176 22.332525850022687 50-51 22.686402027736612 26.768275383383493 28.18056911824866 22.364753470631232 52-53 23.038519032671477 26.822368841986076 27.84420476012287 22.294907365219576 54-55 22.55034775971132 26.699937758089025 28.057625797494683 22.692088684704974 56-57 22.808019867089556 26.931313447932098 27.788364438943763 22.47230224603458 58-59 23.094663445179513 26.626903252303908 27.776795792204386 22.501637510312193 60-61 22.73153984389668 26.602873689323182 28.42805387254568 22.23753259423446 62-63 22.621658556257824 26.737528125760402 28.285717332512977 22.3550959854688 64-65 22.472764198345217 26.842418074443607 28.129414536512247 22.55540319069893 66-67 22.435716806243676 26.978033909522043 27.85944364807205 22.72680563616223 68-69 22.60182720081792 26.760739966891556 28.034765479715347 22.60266735257518 70-71 22.600645306181143 26.808804212424324 27.892349874218613 22.698200607175924 72-73 22.643204973680724 26.71062368120878 27.92715910155783 22.719012243552665 74-75 22.58248806827178 27.106204498673534 27.62855084031034 22.682756592744347 76-77 22.68826425856619 26.856823967432998 27.684150729028595 22.77076104497222 78-79 22.698870623046243 26.866968656856777 27.706204932132998 22.727955787963978 80-81 22.721026525380676 27.121616782645454 27.495084421267357 22.662272270706513 82-83 22.655315578918724 27.13205350820808 27.56167815380976 22.650952759063436 84-85 22.620711897555417 27.042942263431698 27.52293311151571 22.813412727497177 86-87 22.67537532413476 27.02463036357385 27.435114878787175 22.864879433504225 88-89 22.541100707723416 27.032830143794296 27.265160976694514 23.16090817178777 90-91 22.532954275135232 26.83300883262486 27.602599108463394 23.031437783776514 92-93 22.591280749925435 26.711327243802995 27.758408919275862 22.938983086995705 94-95 22.332441090481698 26.742757757519996 27.716145911598478 23.208655240399825 96-97 22.298834082108694 26.605375024318235 27.820834246276966 23.2749566472961 98-99 22.186422542293624 26.693239580542656 27.481152114850833 23.639185762312888 100-101 21.89285013697594 26.755795017934364 27.66668422561329 23.684670619476407 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 242.0 1 179.0 2 232.0 3 306.0 4 222.5 5 211.0 6 365.0 7 743.0 8 1149.0 9 1432.5 10 1447.0 11 1318.0 12 1401.0 13 1536.0 14 1680.0 15 1924.0 16 2133.0 17 2398.5 18 2650.5 19 2795.5 20 3061.5 21 3503.0 22 4036.5 23 4662.5 24 5598.0 25 6746.0 26 8224.5 27 10131.5 28 12040.5 29 14501.0 30 17513.0 31 20719.0 32 24239.5 33 26795.5 34 29272.5 35 31934.5 36 34828.0 37 37902.5 38 40026.0 39 41412.5 40 42955.0 41 45622.0 42 48326.5 43 50831.5 44 52988.0 45 55073.5 46 56444.0 47 59321.0 48 66591.0 49 66519.0 50 59803.5 51 56361.0 52 53734.0 53 52481.5 54 51089.5 55 47452.0 56 43111.0 57 39124.5 58 36045.0 59 32098.5 60 26902.0 61 22303.0 62 18492.5 63 14479.0 64 10717.0 65 8195.0 66 6459.0 67 5365.0 68 4154.0 69 2863.0 70 2128.5 71 1677.5 72 1389.0 73 1204.0 74 1005.5 75 814.5 76 595.0 77 403.0 78 276.0 79 180.0 80 120.5 81 84.5 82 70.5 83 49.0 84 23.5 85 9.0 86 6.0 87 6.0 88 1.5 89 1.5 90 2.0 91 1.5 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0928396705324281 2 0.013313126678277953 3 0.004523877997473091 4 0.013571633992419275 5 0.0 6 0.0 7 0.0 8 0.0 9 0.004717758483079081 10-11 9.694024280299481E-5 12-13 6.462682853532988E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001034029256565278 24-25 0.0 26-27 3.231341426766494E-5 28-29 0.0 30-31 0.0 32-33 0.0038452962978521277 34-35 8.078353566916234E-4 36-37 0.0027143267984838545 38-39 9.694024280299481E-5 40-41 0.0035544755694431425 42-43 0.010340292565652779 44-45 0.002455819484342535 46-47 0.0021326853416658857 48-49 0.00975865110883481 50-51 2.585073141413195E-4 52-53 0.002068058513130556 54-55 0.010081785251511459 56-57 0.004039176783458117 58-59 0.0017126109561862416 60-61 0.006494996267800652 62-63 0.0034575353266401484 64-65 0.016447527862241452 66-67 0.0032959682553018238 68-69 3.554475569443143E-4 70-71 0.0011309694993682728 72-73 0.0 74-75 0.0 76-77 7.755219424239585E-4 78-79 0.010631113294061764 80-81 0.014411782763378561 82-83 0.011665142550627042 84-85 0.008272234052522224 86-87 0.00962939745176415 88-89 0.026690880185091238 90-91 0.022813270472971443 92-93 0.002875893869822179 94-95 0.0077875328385072495 96-97 0.00982327793737014 98-99 0.010049471837243796 100-101 0.011342008407950393 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1547345.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.27598333066021 #Duplication Level Percentage of deduplicated Percentage of total 1 81.51111932593017 51.57696227731012 2 11.310894748190968 14.314159750827676 3 3.187118518132795 6.050041746786277 4 1.343163030052021 3.3995984599972293 5 0.7004492048712485 2.216080610570366 6 0.41591878838026203 1.579060219227471 7 0.28417923187063676 1.2587204237136365 8 0.20245600370308567 1.0248482172406823 9 0.1544432025791945 0.87952909607414 >10 0.7998567683940777 9.319309223184135 >50 0.0577587738428059 2.519863838502152 >100 0.029871015268168124 3.532689376316991 >500 0.0021555246102652596 0.9536464418375995 >1k 4.105761162410018E-4 0.605130007396371 >5k 2.052880581205009E-4 0.7703603110151719 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6103 0.3944175345511182 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5758 0.3721212787064294 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3313 0.2141086829375479 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2824 0.18250616378377155 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1689 0.10915471339617215 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.462682853532988E-5 2 0.0 0.0 0.0 0.0 6.462682853532988E-5 3 0.0 0.0 0.0 0.0 6.462682853532988E-5 4 0.0 0.0 0.0 0.0 1.2925365707065975E-4 5 0.0 0.0 0.0 0.0 1.2925365707065975E-4 6 0.0 0.0 0.0 0.0 1.2925365707065975E-4 7 0.0 0.0 0.0 0.0 1.2925365707065975E-4 8 0.0 0.0 0.0 0.0 1.2925365707065975E-4 9 0.0 0.0 0.0 0.0 1.2925365707065975E-4 10-11 0.0 0.0 0.0 0.0 1.2925365707065975E-4 12-13 0.0 0.0 0.0 0.0 1.2925365707065975E-4 14-15 1.2925365707065975E-4 0.0 0.0 0.0 1.6156707133832467E-4 16-17 2.2619389987365456E-4 0.0 0.0 0.0 1.9388048560598962E-4 18-19 2.908207284089844E-4 0.0 0.0 3.231341426766494E-5 3.2313414267664934E-4 20-21 3.2313414267664934E-4 0.0 0.0 6.462682853532988E-5 3.2313414267664934E-4 22-23 3.2313414267664934E-4 0.0 0.0 1.2925365707065975E-4 3.8776097121197923E-4 24-25 3.2313414267664934E-4 0.0 0.0 2.585073141413195E-4 4.523877997473091E-4 26-27 3.2313414267664934E-4 3.231341426766494E-5 0.0 3.8776097121197923E-4 4.523877997473091E-4 28-29 3.2313414267664934E-4 6.462682853532988E-5 0.0 8.401487709592884E-4 4.523877997473091E-4 30-31 3.2313414267664934E-4 6.462682853532988E-5 0.0 0.001971118270327561 4.523877997473091E-4 32-33 3.2313414267664934E-4 6.462682853532988E-5 0.0 0.003328281669569488 4.523877997473091E-4 34-35 3.2313414267664934E-4 6.462682853532988E-5 0.0 0.005137832868558725 4.523877997473091E-4 36-37 4.523877997473091E-4 6.462682853532988E-5 0.0 0.00798141332411324 4.523877997473091E-4 38-39 4.523877997473091E-4 6.462682853532988E-5 0.0 0.012020590107571356 4.523877997473091E-4 40-41 4.523877997473091E-4 6.462682853532988E-5 0.0 0.016189020548100133 4.523877997473091E-4 42-43 4.523877997473091E-4 6.462682853532988E-5 0.0 0.02291021071577444 4.8470121401497407E-4 44-45 4.523877997473091E-4 6.462682853532988E-5 0.0 0.02827423748420682 5.49328042550304E-4 46-47 4.8470121401497407E-4 6.462682853532988E-5 0.0 0.03848527639278894 5.816414568179688E-4 48-49 5.17014628282639E-4 6.462682853532988E-5 0.0 0.05066743357169862 6.462682853532987E-4 50-51 5.17014628282639E-4 6.462682853532988E-5 0.0 0.05709780301096394 6.462682853532987E-4 52-53 5.17014628282639E-4 6.462682853532988E-5 0.0 0.06811667727623769 6.785816996209636E-4 54-55 5.17014628282639E-4 6.462682853532988E-5 0.0 0.08714927827989233 7.755219424239585E-4 56-57 5.17014628282639E-4 6.462682853532988E-5 0.0 0.11558508283543747 8.724621852269533E-4 58-59 5.17014628282639E-4 6.462682853532988E-5 0.0 0.1455396178615629 9.047755994946183E-4 60-61 5.17014628282639E-4 6.462682853532988E-5 0.0 0.18631914666735602 9.047755994946183E-4 62-63 5.17014628282639E-4 6.462682853532988E-5 0.0 0.22341494624663538 9.047755994946183E-4 64-65 5.17014628282639E-4 6.462682853532988E-5 0.0 0.2600260446118997 9.047755994946183E-4 66-67 5.816414568179688E-4 6.462682853532988E-5 0.0 0.32174466586313977 9.047755994946183E-4 68-69 5.816414568179688E-4 6.462682853532988E-5 0.0 0.40094484423318655 9.047755994946183E-4 70-71 7.432085281562935E-4 6.462682853532988E-5 0.0 0.4466360120076648 9.047755994946183E-4 72-73 7.755219424239585E-4 6.462682853532988E-5 0.0 0.504864784517997 9.047755994946183E-4 74-75 8.401487709592884E-4 6.462682853532988E-5 0.0 0.5667449728405753 9.047755994946183E-4 76-77 8.401487709592884E-4 6.462682853532988E-5 0.0 0.6339568745173183 9.047755994946183E-4 78-79 8.401487709592884E-4 6.462682853532988E-5 0.0 0.7095702639036543 0.001001715842297613 80-81 9.69402428029948E-4 6.462682853532988E-5 0.0 0.828322061337323 0.001034029256565278 82-83 9.69402428029948E-4 6.462682853532988E-5 0.0 0.9670435487884086 0.001034029256565278 84-85 0.001001715842297613 6.462682853532988E-5 0.0 1.1662557477485629 0.001066342670832943 86-87 0.001066342670832943 6.462682853532988E-5 0.0 1.4580458785855774 0.0011309694993682728 88-89 0.001098656085100608 6.462682853532988E-5 0.0 1.7646032397429146 0.0011632829136359376 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8125 0.0 25.297739 1 GTACATG 6500 0.0 24.677116 1 GTACACG 275 1.8189894E-12 24.44875 1 GTATCAA 10815 0.0 24.334103 1 TACATGG 6765 0.0 24.014692 2 ACATGGG 6855 0.0 23.004948 3 GAGTACT 6950 0.0 22.617031 12-13 GTACTTT 7640 0.0 20.418997 14-15 AGTACTT 7230 0.0 20.32894 12-13 TATCAAC 13060 0.0 20.259594 2 TAAGGTG 680 0.0 20.252634 5 ATCAACG 13075 0.0 20.235044 3 GTGTAGC 950 0.0 20.22077 1 TCAACGC 13105 0.0 20.187416 4 CAACGCA 13220 0.0 20.008575 5 AACGCAG 13420 0.0 19.745771 6 CATGGGG 3180 0.0 19.416851 4 GTAAGGT 595 0.0 19.158295 4 GTACCTG 1410 0.0 19.073492 1 GTATAGG 355 6.730261E-11 18.939173 1 >>END_MODULE