FastQCFastQC Report
Thu 26 May 2016
SRR936429_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936429_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1544821
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48170.3118160615372267No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40060.2593180698605211No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT24790.160471666296613No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22580.14616580173366364No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16100.10421919432736866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG70350.025.8699131
GTATCAA100400.025.3683031
TACATGG71950.024.5689472
GAGTACT52550.023.65068412-13
ACATGGG71700.023.1302683
GGTATCA74650.022.9156951
TCAACGC114550.022.0300774
TATCAAC115600.021.9511782
ATCAACG114900.021.919483
CAACGCA116400.021.7608975
GTACTTT57200.021.72803314-15
AACGCAG118100.021.5267416
GTACCTG13850.020.928611
AGTACTT55100.020.39972312-13
GTGTAGC8900.020.2886851
ACCGCTC2151.0749845E-619.8919988
ATACCGT1200.004752769619.7999066
GTAAGGT5300.019.7271084
ACGCAGA127900.019.6915427
CATGGGG36050.019.6425384
Produced by FastQC (version 0.11.5)