##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936429_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1544821 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.35597263372261 31.0 31.0 34.0 30.0 34.0 2 31.396084077054883 31.0 31.0 34.0 28.0 34.0 3 31.44620056304258 31.0 31.0 34.0 28.0 34.0 4 35.07262330069309 37.0 35.0 37.0 32.0 37.0 5 35.03739786033463 37.0 35.0 37.0 32.0 37.0 6 35.169035765308735 37.0 35.0 37.0 32.0 37.0 7 35.02789255195262 37.0 35.0 37.0 32.0 37.0 8 34.97437955594855 37.0 35.0 37.0 32.0 37.0 9 36.44272443215104 39.0 37.0 39.0 32.0 39.0 10-11 36.35659244663297 39.0 37.0 39.0 32.0 39.0 12-13 36.2519783845507 39.0 35.0 39.0 32.0 39.0 14-15 37.317905763839306 40.0 37.0 41.0 32.0 41.0 16-17 37.18529072300286 39.5 36.5 41.0 31.0 41.0 18-19 37.13367924180213 39.0 36.0 41.0 31.0 41.0 20-21 37.04631119074637 39.0 36.0 41.0 31.0 41.0 22-23 36.917500150502875 39.0 36.0 41.0 31.0 41.0 24-25 37.05205392728348 39.0 36.0 41.0 31.0 41.0 26-27 37.036940849457636 39.0 36.0 41.0 31.0 41.0 28-29 36.94579048316925 39.0 36.0 41.0 30.5 41.0 30-31 36.76279355342787 39.0 36.0 41.0 30.0 41.0 32-33 36.58871545635384 39.0 35.5 41.0 30.0 41.0 34-35 36.36859286609905 39.0 35.0 40.5 30.0 41.0 36-37 36.179637640865835 39.0 35.0 40.0 30.0 41.0 38-39 35.97955717846922 38.5 35.0 40.0 29.0 41.0 40-41 35.797911214308975 38.0 35.0 40.0 28.5 41.0 42-43 35.572452730769456 38.0 35.0 40.0 27.0 41.0 44-45 35.30633905157944 38.0 34.5 40.0 26.5 41.0 46-47 35.02989537299143 38.0 34.0 40.0 25.5 41.0 48-49 34.93714967624081 38.0 34.0 40.0 24.5 41.0 50-51 34.01774089036853 37.5 33.0 39.5 23.5 40.5 52-53 34.15635080051346 38.0 33.5 39.5 24.0 40.5 54-55 34.58039507489865 38.0 34.0 40.0 24.0 41.0 56-57 34.4991876081436 38.0 34.0 40.0 24.0 41.0 58-59 34.23143814072957 38.0 34.0 40.0 23.0 41.0 60-61 33.91595951893456 37.5 33.0 40.0 22.5 41.0 62-63 33.582065171304635 37.0 33.0 40.0 22.0 41.0 64-65 33.23362512549998 36.5 32.5 39.5 21.5 41.0 66-67 32.85110022455676 36.0 32.0 39.0 20.5 41.0 68-69 32.444834061680936 35.5 31.5 39.0 19.5 40.5 70-71 32.011657661308334 35.0 31.0 38.5 18.0 40.0 72-73 31.615473572666346 35.0 31.0 37.5 16.5 40.0 74-75 31.191415057149015 35.0 31.0 37.0 13.0 39.0 76-77 30.763492016227126 35.0 30.0 36.5 9.5 39.0 78-79 30.318609728894156 34.0 30.0 36.0 7.0 38.5 80-81 29.85680930023608 34.0 29.5 35.5 6.5 37.0 82-83 29.377138192709708 34.0 29.0 35.0 2.0 37.0 84-85 29.063107958786162 34.0 29.0 35.0 2.0 36.5 86-87 28.74190796215225 34.0 29.0 35.0 2.0 36.0 88-89 28.338727593682375 34.0 28.5 35.0 2.0 36.0 90-91 27.932253316079986 34.0 27.0 35.0 2.0 35.0 92-93 27.571413128122934 33.5 26.5 35.0 2.0 35.0 94-95 27.11433816603995 33.0 26.0 35.0 2.0 35.0 96-97 26.531381305665835 33.0 25.0 35.0 2.0 35.0 98-99 25.82323162359911 33.0 23.5 35.0 2.0 35.0 100-101 23.356907046188525 29.5 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4224382675706657 1101 2 0.43277393271819875 1101 3 0.522972957441727 1101 4 0.5100477091859617 1101 5 0.4317194154293489 1101 6 0.2637428487615452 1101 7 0.3587658902897246 1101 8 0.49313701160209433 1101 9 0.6756699835454896 1101 10-11 0.5903382551034895 1101 12-13 0.7211654470401001 1101 14-15 0.9338525042105843 1101 16-17 0.9070201026601197 1101 18-19 0.8748825078200326 1101 20-21 0.7873976073962368 1101 22-23 0.8008951873164634 1101 24-25 0.8135998511504781 1101 26-27 0.7451806062732729 1101 28-29 0.6501617850484038 1101 30-31 0.686201867203593 1101 32-33 0.627632360195463 1101 34-35 0.5437443168249914 1101 36-37 0.4725362855161208 1101 38-39 0.4428546533654796 1101 40-41 0.5370781572350438 1101 42-43 0.703026841578783 1101 44-45 0.6688079763650805 1101 46-47 0.7973774392332658 1101 48-49 0.9172362311255995 1101 50-51 0.9088502979990736 1101 52-53 0.8735737544913462 1101 54-55 0.9310027200224411 1101 56-57 0.66701268330457 1101 58-59 0.7773891407408797 1101 60-61 0.6195602367192734 1101 62-63 0.7574240543729687 1101 64-65 0.6917157719776625 1101 66-67 0.8445979982830565 1101 68-69 0.7383494017442231 1101 70-71 0.8202787888371113 1101 72-73 0.7502810510738556 1101 74-75 0.6293497981392839 1101 76-77 0.605518515928356 1101 78-79 0.42407904385817474 1101 80-81 0.6929799080993639 1101 82-83 0.6961279830481644 1101 84-85 0.6288904036676541 1101 86-87 0.4815386576991578 1101 88-89 0.726963633770918 1101 90-91 0.6394915461705111 1101 92-93 0.7424717163100176 1101 94-95 0.9692480547576778 1101 96-97 0.6625503644745052 1101 98-99 0.4585035213884403 1101 100-101 0.6553949637919061 1102 1 0.34523648367633797 1102 2 0.33497597707532023 1102 3 0.31504612481021255 1102 4 0.26753846582117546 1102 5 0.2920365916940142 1102 6 0.34402667353199234 1102 7 0.30225220618758186 1102 8 0.3728108631603675 1102 9 0.5062475184153286 1102 10-11 0.417317419918831 1102 12-13 0.5384097384618016 1102 14-15 0.4791684333181223 1102 16-17 0.3636645390829898 1102 18-19 0.5332161526872881 1102 20-21 0.6251864376486509 1102 22-23 0.6938408437470471 1102 24-25 0.39971574422223455 1102 26-27 0.31367052268616646 1102 28-29 0.3759503897706864 1102 30-31 0.2956202936283745 1102 32-33 0.23517622059721077 1102 34-35 0.09864435733461363 1102 36-37 0.15461739546757514 1102 38-39 0.06785135927724895 1102 40-41 0.05680514933106906 1102 42-43 0.2931786821927176 1102 44-45 0.1599989715842085 1102 46-47 0.25572514909119803 1102 48-49 0.259144357013092 1102 50-51 0.23793308287947212 1102 52-53 0.14032963221723804 1102 54-55 0.3789470863961384 1102 56-57 0.4502353250591469 1102 58-59 0.48877354030673104 1102 60-61 0.2576261993805531 1102 62-63 0.36723240891614495 1102 64-65 0.4062926792853929 1102 66-67 0.43113660714210056 1102 68-69 0.31804917701079916 1102 70-71 0.26859898769584944 1102 72-73 0.3150025305515385 1102 74-75 0.2505178542472599 1102 76-77 0.3437782322629239 1102 78-79 0.214400369141881 1102 80-81 0.5074815263393582 1102 82-83 0.5209845293360758 1102 84-85 0.41868681982223066 1102 86-87 0.37885595224457447 1102 88-89 0.30253798486618066 1102 90-91 0.319112048820795 1102 92-93 0.3747385515522872 1102 94-95 0.46272422792804946 1102 96-97 0.5667299342635204 1102 98-99 0.7020086518925552 1102 100-101 0.6051092514323031 1103 1 0.2677804237207937 1103 2 0.3576792197237175 1103 3 0.27827679480095213 1103 4 0.2861643168257544 1103 5 0.4251727617142649 1103 6 0.26025543530703743 1103 7 0.31576850931254086 1103 8 0.24310163160892273 1103 9 0.45822334109178087 1103 10-11 0.46798520552874123 1103 12-13 0.46881154834552063 1103 14-15 0.36152194727829823 1103 16-17 0.4648850600867007 1103 18-19 0.31226910365043636 1103 20-21 0.3928813256739332 1103 22-23 0.4310940138448913 1103 24-25 0.5168729672335957 1103 26-27 0.4591098751931426 1103 28-29 0.010596444072596967 1103 30-31 0.12271405026660176 1103 32-33 0.24189287618330013 1103 34-35 0.2470591361340979 1103 36-37 0.3207731273202725 1103 38-39 0.43225179634823263 1103 40-41 0.06060555158737202 1103 42-43 0.1736281749980364 1103 44-45 0.18779408512587992 1103 46-47 0.27915689330340854 1103 48-49 0.10021207095630302 1103 50-51 0.1551411197362924 1103 52-53 0.3409814036759826 1103 54-55 0.3363392575285431 1103 56-57 0.42043791830300137 1103 58-59 0.22147204012981092 1103 60-61 0.051828627239721925 1103 62-63 0.15079718593295155 1103 64-65 0.2942689729486787 1103 66-67 0.19710768939011558 1103 68-69 0.12342735516067194 1103 70-71 0.17632407041066145 1103 72-73 0.21924075280998068 1103 74-75 -0.009653678434339952 1103 76-77 0.18271886545257487 1103 78-79 0.13684498849195137 1103 80-81 0.1046914687153766 1103 82-83 -0.08864784028191508 1103 84-85 0.0030794490582302103 1103 86-87 0.16784157186639348 1103 88-89 0.1707431685375589 1103 90-91 0.17017834560900624 1103 92-93 0.32373685066667335 1103 94-95 0.2514328581623495 1103 96-97 -0.005368051368986215 1103 98-99 0.12592375681745338 1103 100-101 0.5052011717612253 1104 1 0.08735117907035672 1104 2 0.273151999513761 1104 3 0.1396962184779298 1104 4 0.13791506887103822 1104 5 0.304240122845961 1104 6 0.18865831736232508 1104 7 0.2898232035688224 1104 8 0.2306046651737219 1104 9 0.3672310697492094 1104 10-11 0.3169163196180662 1104 12-13 0.18750655352055645 1104 14-15 0.24694402141788885 1104 16-17 0.13757743064554973 1104 18-19 0.34500959454357627 1104 20-21 0.3252902739784673 1104 22-23 0.07764270479749058 1104 24-25 0.07595947320373853 1104 26-27 -0.03057468395469698 1104 28-29 0.10818405943975762 1104 30-31 0.0834056531538252 1104 32-33 -0.16391798138162272 1104 34-35 -0.32709116493345647 1104 36-37 -0.08021307835996794 1104 38-39 -0.20152701738194168 1104 40-41 -0.10542515469106917 1104 42-43 -0.238656097094605 1104 44-45 -0.06626179467833282 1104 46-47 -0.14702602089666783 1104 48-49 -0.20317756905675566 1104 50-51 -0.18819679047722815 1104 52-53 3.966644363728733E-4 1104 54-55 0.04413778598183882 1104 56-57 -0.12133768036202497 1104 58-59 -0.2557875951616069 1104 60-61 -0.08260546583224482 1104 62-63 0.024178318759219053 1104 64-65 -0.19917764672842964 1104 66-67 -0.03786659096908096 1104 68-69 0.016566201898378097 1104 70-71 -0.14647979741013017 1104 72-73 -0.024227802438460344 1104 74-75 -0.005259814760027126 1104 76-77 0.03860260193578924 1104 78-79 -0.09242956474868791 1104 80-81 -0.01181942849497375 1104 82-83 0.013886209740576305 1104 84-85 0.19668089425289992 1104 86-87 0.24621362674082903 1104 88-89 0.26849731817542377 1104 90-91 0.21732691207906996 1104 92-93 0.2552507689700967 1104 94-95 0.1974020978886557 1104 96-97 0.09549023838421178 1104 98-99 0.34892033486832474 1104 100-101 0.6487145372002168 1105 1 0.19242460753480728 1105 2 0.23210129791682022 1105 3 0.24543944766626424 1105 4 0.20654854464351047 1105 5 0.327911070611016 1105 6 0.33670329563986456 1105 7 0.36552821786226275 1105 8 0.38169673157135975 1105 9 0.3401490117315049 1105 10-11 0.3182605270601613 1105 12-13 0.15828567974857322 1105 14-15 0.1361240949949334 1105 16-17 0.26716633822255886 1105 18-19 0.16690693733058737 1105 20-21 0.24617296078867668 1105 22-23 0.2452628618173236 1105 24-25 0.1467931284614039 1105 26-27 0.3342303190466325 1105 28-29 0.14291251378692493 1105 30-31 -0.005109374274134382 1105 32-33 0.25978832853344613 1105 34-35 0.237891540300744 1105 36-37 0.3436889120239002 1105 38-39 0.258370075789891 1105 40-41 0.10738099535141998 1105 42-43 0.2838125375698084 1105 44-45 0.09331005586160046 1105 46-47 0.30789139808240407 1105 48-49 0.4707478970401553 1105 50-51 0.6242007632940343 1105 52-53 0.529447264998602 1105 54-55 0.4801535414540581 1105 56-57 0.6399975231040571 1105 58-59 0.7288365557010508 1105 60-61 0.6080629382352924 1105 62-63 0.5778778236891142 1105 64-65 0.7764397050922796 1105 66-67 0.8588523066827563 1105 68-69 0.8222354380164987 1105 70-71 0.8586390966757698 1105 72-73 0.8502377536387797 1105 74-75 0.8990589735365866 1105 76-77 0.6983628525018801 1105 78-79 0.6644877472524513 1105 80-81 0.5260155461572005 1105 82-83 0.6082875493420232 1105 84-85 0.6789687659269283 1105 86-87 0.7645514273933891 1105 88-89 0.8262659962253345 1105 90-91 0.8546483926536617 1105 92-93 0.8004774730394146 1105 94-95 0.8257704169681688 1105 96-97 1.128175859955718 1105 98-99 1.05464395143564 1105 100-101 1.0797714197443895 1106 1 0.04908709522174348 1106 2 0.1518540131875774 1106 3 0.20391083658002884 1106 4 0.17150652031161684 1106 5 0.153496021166859 1106 6 0.06796632829986748 1106 7 0.10162500814997344 1106 8 0.18318325436172245 1106 9 0.42403520702631425 1106 10-11 0.18861930099868118 1106 12-13 0.3384872302353372 1106 14-15 0.4071004411167962 1106 16-17 0.5614581930499227 1106 18-19 0.510935580398808 1106 20-21 0.4133005945053725 1106 22-23 0.3206879024344502 1106 24-25 0.192477479035162 1106 26-27 0.025159907502406043 1106 28-29 0.2744752176720979 1106 30-31 0.31068972767165093 1106 32-33 0.2520339757849115 1106 34-35 0.08674562567985333 1106 36-37 -0.03114414200006621 1106 38-39 0.10897165697937083 1106 40-41 6.372734156130377E-4 1106 42-43 -0.016204732703577918 1106 44-45 0.17544107966899247 1106 46-47 0.07659830235392207 1106 48-49 -0.05297666128859646 1106 50-51 -0.03151249899664066 1106 52-53 0.25344239094366827 1106 54-55 0.29269016083002697 1106 56-57 0.06638138371376812 1106 58-59 0.252383294177946 1106 60-61 0.2840831636443397 1106 62-63 0.3445094167758853 1106 64-65 0.20437277318832514 1106 66-67 0.2828595857831644 1106 68-69 0.37127214054563495 1106 70-71 0.5872712910239137 1106 72-73 0.5131066658674115 1106 74-75 0.22186245061829268 1106 76-77 0.3253140735741731 1106 78-79 0.5431877434146131 1106 80-81 0.5768083796333876 1106 82-83 0.6610434372259668 1106 84-85 0.5267373826419934 1106 86-87 0.6821274485782602 1106 88-89 0.6697800491107486 1106 90-91 0.7395698448917578 1106 92-93 0.7640109709733771 1106 94-95 0.8804452241167837 1106 96-97 0.8678981283741436 1106 98-99 1.2166218454857116 1106 100-101 1.687484195908855 1107 1 0.16494162019739633 1107 2 0.3177928180428289 1107 3 0.08076118080434824 1107 4 0.1042338380734833 1107 5 0.22905980988965524 1107 6 0.1973434393707123 1107 7 0.16724794270628962 1107 8 0.28930289919992447 1107 9 0.3399841150380567 1107 10-11 0.21930128361604062 1107 12-13 0.38805168641827237 1107 14-15 0.1412635803695821 1107 16-17 0.11246186016981596 1107 18-19 0.27973657689594944 1107 20-21 0.3534191860080327 1107 22-23 0.4189433806266223 1107 24-25 0.47088527371816724 1107 26-27 0.2792689590180757 1107 28-29 0.2982922684107976 1107 30-31 0.37451788536433384 1107 32-33 0.2349055405487519 1107 34-35 0.403406903996391 1107 36-37 0.4354277722678077 1107 38-39 0.2748563610611754 1107 40-41 0.1854165096691318 1107 42-43 0.3600667984265371 1107 44-45 0.44205979436498666 1107 46-47 0.5360807339425193 1107 48-49 0.19746331488099145 1107 50-51 0.1573259248066634 1107 52-53 0.5154165868301845 1107 54-55 0.5617955556548608 1107 56-57 0.47016315814091314 1107 58-59 0.5785852113007763 1107 60-61 0.5425352688162448 1107 62-63 0.4786940007930909 1107 64-65 0.44510133461616874 1107 66-67 0.44049555145590347 1107 68-69 0.33385265618467486 1107 70-71 0.5319855150949842 1107 72-73 0.4655436123936809 1107 74-75 0.4409731903388199 1107 76-77 0.3800728558244266 1107 78-79 0.5766950248572691 1107 80-81 0.5730969362117158 1107 82-83 0.646199944897198 1107 84-85 0.7109707225369455 1107 86-87 0.6380855080943242 1107 88-89 0.5168515025443234 1107 90-91 0.33846605875016067 1107 92-93 0.5923495427390328 1107 94-95 0.3463809654342569 1107 96-97 0.3821592226176911 1107 98-99 0.6449347356793922 1107 100-101 1.124667005681406 1108 1 -0.020229956444257624 1108 2 0.08203513891026404 1108 3 0.1695931085748903 1108 4 0.14654364887702798 1108 5 0.333662603845994 1108 6 0.2435706005204139 1108 7 -0.043145558692309294 1108 8 0.0808955460019618 1108 9 0.21565231637388393 1108 10-11 -0.09107566654755317 1108 12-13 0.22971805532002065 1108 14-15 0.1573813145965275 1108 16-17 0.24031239746133792 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2316 80-81 -2.8336706125594304 2316 82-83 -2.8891554508710584 2316 84-85 -2.6315765751585793 2316 86-87 -3.2414453459164 2316 88-89 -3.4490307983927906 2316 90-91 -3.0774442377076667 2316 92-93 -3.918509772402121 2316 94-95 -4.211488722784047 2316 96-97 -5.312247567032806 2316 98-99 -6.215021355556537 2316 100-101 -6.506875105475544 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3695.0 3 1441.0 4 1513.0 5 1503.0 6 1837.0 7 2055.0 8 2563.0 9 3199.0 10 4009.0 11 5122.0 12 6356.0 13 7751.0 14 8717.0 15 10103.0 16 12375.0 17 14970.0 18 13146.0 19 9913.0 20 10036.0 21 10504.0 22 11974.0 23 13500.0 24 15593.0 25 18339.0 26 21119.0 27 24894.0 28 29662.0 29 35891.0 30 43455.0 31 53125.0 32 66496.0 33 84893.0 34 111947.0 35 152222.0 36 212126.0 37 276324.0 38 211786.0 39 30651.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.596348264402444 18.422511351774194 14.194833485726424 22.786306898096942 2 15.683524600566162 19.76967643851866 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22.529686102660655 46-47 22.7942328332084 26.90887058247789 27.723758329481736 22.573138254831974 48-49 22.84347488348006 26.989772138788194 27.552725271879858 22.614027705851893 50-51 22.662106032795524 27.180027116524347 27.594898934467537 22.562967916212596 52-53 23.00430582038291 27.245067953503742 27.300513118623847 22.4501131074895 54-55 22.52836871283065 27.081313663107515 27.503854299546802 22.886463324515027 56-57 22.773449993590333 27.39388116347759 27.214442857494326 22.618225985437753 58-59 22.978294745278703 27.0194393677787 27.2637667198179 22.738499167124697 60-61 22.737867918036038 26.98496654106145 27.82690239144093 22.450263149461577 62-63 22.643680542023876 27.157740937083464 27.686959978093245 22.511618542799418 64-65 22.473736439367407 27.267463350122767 27.5090771034314 22.749723107078427 66-67 22.43187892492658 27.403573975307093 27.239334379710677 22.925212720055647 68-69 22.51123227592342 27.2406848750554 27.494772730730645 22.753310118290536 70-71 22.6282574772558 27.20370393747275 27.261772168615384 22.906266416656067 72-73 22.604961072303173 27.180769905495062 27.39707804376914 22.817190978432624 74-75 22.520905317160405 27.644993971565206 27.137796263179613 22.69630444809477 76-77 22.736943503335073 27.330075136618653 27.10275231198933 22.830229048056943 78-79 22.651848221854596 27.391605650524696 27.150853395212078 22.805692732408627 80-81 22.662004173067725 27.597922400103066 26.98847065419278 22.75160277263643 82-83 22.677712454963565 27.6131652210447 27.04571665507342 22.663405668918312 84-85 22.584761647017846 27.53915898421339 26.93036812973726 22.945711239031503 86-87 22.653103236656996 27.502942238898147 27.05872566422095 22.785228860223906 88-89 22.61380199474141 27.413015310625966 26.87314103636828 23.10004165826434 90-91 22.58422231155701 27.403672177587197 27.038208746911312 22.97389676394448 92-93 22.68104145105731 27.378182669264607 27.181783249754865 22.758992629923217 94-95 22.4373514002567 27.319661212816683 27.174545319178893 23.068442067747725 96-97 22.31836329348214 27.284624542699603 27.280254657488396 23.11675750632986 98-99 22.313871132114933 27.39852416442805 26.893597266654623 23.394007436802394 100-101 22.274963153300405 27.28284407305619 27.140767343851586 23.30142542979182 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 172.0 1 131.5 2 140.0 3 201.0 4 196.5 5 195.0 6 309.0 7 550.5 8 894.5 9 1116.5 10 1100.5 11 1078.5 12 1137.0 13 1288.5 14 1432.0 15 1559.0 16 1742.5 17 1892.0 18 2047.0 19 2229.5 20 2484.0 21 2938.0 22 3467.5 23 4067.0 24 4918.0 25 6111.5 26 7617.5 27 9599.5 28 11625.0 29 14048.5 30 17279.0 31 20662.0 32 24341.5 33 27022.5 34 29461.0 35 32140.5 36 35205.0 37 38394.5 38 40199.0 39 41375.0 40 42992.0 41 45957.5 42 48598.0 43 50941.0 44 53427.0 45 55968.0 46 57514.0 47 59635.5 48 66322.5 49 66378.5 50 60053.5 51 56775.5 52 53914.0 53 52408.5 54 51285.5 55 48000.0 56 43763.5 57 39594.0 58 35914.0 59 31840.0 60 26900.5 61 22310.0 62 18525.0 63 14693.5 64 11129.5 65 8700.0 66 6649.5 67 5299.5 68 4098.5 69 2871.0 70 2190.0 71 1750.0 72 1440.0 73 1250.0 74 997.5 75 777.0 76 587.5 77 372.5 78 229.0 79 147.5 80 98.5 81 71.5 82 47.0 83 26.5 84 17.0 85 18.0 86 15.5 87 9.0 88 7.5 89 7.0 90 5.0 91 3.5 92 4.5 93 3.0 94 0.5 95 0.0 96 0.5 97 0.5 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03683274631818185 2 0.024921981252196856 3 0.02330367078127498 4 0.05223906200135809 5 0.061754727570378705 6 0.05314531586507434 7 0.05579934503738621 8 0.046413144306039336 9 0.04699573607557121 10-11 0.05583171124680465 12-13 0.048937708640677466 14-15 0.04181714256862122 16-17 0.05343661174984027 18-19 0.05728819067063433 20-21 0.012460990626098428 22-23 0.02171772651977155 24-25 0.005534621810552809 26-27 0.010842680155176554 28-29 0.005825917695318745 30-31 0.009256735893673118 32-33 0.004304705852652184 34-35 0.007670791632169682 36-37 0.009289102103091555 38-39 0.011425271924708429 40-41 0.0018772401462693736 42-43 0.0021038036121984356 44-45 0.0021038036121984356 46-47 4.5312693185812467E-4 48-49 0.001359380795574374 50-51 4.207607224396871E-4 52-53 0.0033337195700990597 54-55 0.007606059213332807 56-57 0.01806034485548811 58-59 0.011133976039942492 60-61 0.01077794773633968 62-63 0.005955382532992496 64-65 0.0 66-67 0.00818865098286468 68-69 0.017898513808395924 70-71 0.005599354229389683 72-73 0.0022009022404537483 74-75 0.0037868465019571845 76-77 0.006570340511942808 78-79 0.005146227297531558 80-81 0.009968792500878744 82-83 0.006440875674269058 84-85 0.018384006949672488 86-87 0.005113861088113122 88-89 0.007088199862637807 90-91 0.0045312693185812465 92-93 0.017121724782353423 94-95 0.012525723044935303 96-97 0.010227722176226244 98-99 0.022009022404537483 100-101 0.03786846501957185 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1544821.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.57539011726873 #Duplication Level Percentage of deduplicated Percentage of total 1 82.58293376937853 54.15408098955562 2 10.837959013405637 14.214067807580872 3 2.9559119044193585 5.815052288537345 4 1.20189051736286 3.1525775821726194 5 0.6462364320963951 2.1188603071356478 6 0.3912606582054997 1.5394242179618987 7 0.2650810003644888 1.2167953008104042 8 0.18173805929328996 0.9534035309850243 9 0.13108599438266502 0.773641369849802 >10 0.7242804961261852 8.68253266047049 >50 0.05487306827555022 2.4907628857017188 >100 0.02456964245409757 3.1171387266118673 >500 0.001485984705963445 0.6431305768921951 >1k 6.934595294496075E-4 1.1285317557344658 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4817 0.3118160615372267 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4006 0.2593180698605211 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2479 0.160471666296613 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2258 0.14616580173366364 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1610 0.10421919432736866 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 6.473241883687495E-5 0.0 0.0 0.0 0.0 3 6.473241883687495E-5 0.0 0.0 0.0 0.0 4 6.473241883687495E-5 0.0 0.0 0.0 0.0 5 6.473241883687495E-5 0.0 0.0 0.0 0.0 6 6.473241883687495E-5 0.0 0.0 0.0 0.0 7 6.473241883687495E-5 0.0 0.0 0.0 0.0 8 6.473241883687495E-5 0.0 0.0 0.0 0.0 9 6.473241883687495E-5 0.0 0.0 0.0 0.0 10-11 6.473241883687495E-5 0.0 0.0 0.0 0.0 12-13 6.473241883687495E-5 0.0 0.0 0.0 0.0 14-15 6.473241883687495E-5 0.0 0.0 0.0 0.0 16-17 6.473241883687495E-5 0.0 0.0 0.0 0.0 18-19 6.473241883687495E-5 0.0 0.0 0.0 0.0 20-21 6.473241883687495E-5 0.0 0.0 6.473241883687495E-5 0.0 22-23 6.473241883687495E-5 0.0 0.0 1.294648376737499E-4 0.0 24-25 1.294648376737499E-4 0.0 0.0 1.9419725651062485E-4 0.0 26-27 1.294648376737499E-4 0.0 0.0 3.2366209418437477E-4 0.0 28-29 1.294648376737499E-4 3.2366209418437474E-5 0.0 9.062538637162493E-4 0.0 30-31 1.294648376737499E-4 6.473241883687495E-5 0.0 0.0016183104709218737 0.0 32-33 1.294648376737499E-4 6.473241883687495E-5 0.0 0.0023950994969643732 0.0 34-35 1.9419725651062485E-4 6.473241883687495E-5 0.0 0.004110508596141559 3.2366209418437474E-5 36-37 2.589296753474998E-4 6.473241883687495E-5 0.0 0.00692636881554562 6.473241883687495E-5 38-39 3.883945130212497E-4 6.473241883687495E-5 0.0 0.011943131275403428 6.473241883687495E-5 40-41 3.883945130212497E-4 6.473241883687495E-5 0.0 0.016733330269332176 6.473241883687495E-5 42-43 3.883945130212497E-4 6.473241883687495E-5 0.0 0.02330367078127498 6.473241883687495E-5 44-45 3.883945130212497E-4 6.473241883687495E-5 0.0 0.029809278874380916 6.473241883687495E-5 46-47 3.883945130212497E-4 6.473241883687495E-5 0.0 0.042561565385245276 6.473241883687495E-5 48-49 3.883945130212497E-4 6.473241883687495E-5 0.0 0.05660850027284715 6.473241883687495E-5 50-51 3.883945130212497E-4 6.473241883687495E-5 0.0 0.06376143255432183 6.473241883687495E-5 52-53 3.883945130212497E-4 6.473241883687495E-5 0.0 0.07525143689786713 9.709862825531242E-5 54-55 3.883945130212497E-4 6.473241883687495E-5 0.0 0.09570688125031962 1.6183104709218738E-4 56-57 3.883945130212497E-4 6.473241883687495E-5 0.0 0.12713447059562238 1.9419725651062485E-4 58-59 3.883945130212497E-4 6.473241883687495E-5 0.0 0.1610866242755633 1.9419725651062485E-4 60-61 3.883945130212497E-4 6.473241883687495E-5 0.0 0.20024973767187265 1.9419725651062485E-4 62-63 3.883945130212497E-4 6.473241883687495E-5 0.0 0.23902445655516075 2.589296753474998E-4 64-65 3.883945130212497E-4 6.473241883687495E-5 0.0 0.2812947260556401 2.589296753474998E-4 66-67 3.883945130212497E-4 6.473241883687495E-5 0.0 0.34725706085041563 2.589296753474998E-4 68-69 3.883945130212497E-4 6.473241883687495E-5 0.0 0.4287875423754597 2.589296753474998E-4 70-71 3.883945130212497E-4 6.473241883687495E-5 0.0 0.4730968830693006 2.589296753474998E-4 72-73 3.883945130212497E-4 6.473241883687495E-5 0.0 0.5324565111427149 2.589296753474998E-4 74-75 3.883945130212497E-4 6.473241883687495E-5 0.0 0.6001018888272492 2.589296753474998E-4 76-77 3.883945130212497E-4 6.473241883687495E-5 0.0 0.6648666738735426 2.589296753474998E-4 78-79 3.883945130212497E-4 6.473241883687495E-5 0.0 0.7407654349597785 2.589296753474998E-4 80-81 6.473241883687495E-4 6.473241883687495E-5 0.0 0.8641454252628622 2.589296753474998E-4 82-83 7.120566072056245E-4 6.473241883687495E-5 0.0 0.9999216737732073 3.2366209418437477E-4 84-85 7.44422816624062E-4 6.473241883687495E-5 0.0 1.1950899165663853 3.2366209418437477E-4 86-87 7.767890260424994E-4 6.473241883687495E-5 0.0 1.48146613750072 3.5602830360281226E-4 88-89 7.767890260424994E-4 6.473241883687495E-5 0.0 1.7898513808395924 4.5312693185812467E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7035 0.0 25.869913 1 GTATCAA 10040 0.0 25.368303 1 TACATGG 7195 0.0 24.568947 2 GAGTACT 5255 0.0 23.650684 12-13 ACATGGG 7170 0.0 23.130268 3 GGTATCA 7465 0.0 22.915695 1 TCAACGC 11455 0.0 22.030077 4 TATCAAC 11560 0.0 21.951178 2 ATCAACG 11490 0.0 21.91948 3 CAACGCA 11640 0.0 21.760897 5 GTACTTT 5720 0.0 21.728033 14-15 AACGCAG 11810 0.0 21.526741 6 GTACCTG 1385 0.0 20.92861 1 AGTACTT 5510 0.0 20.399723 12-13 GTGTAGC 890 0.0 20.288685 1 ACCGCTC 215 1.0749845E-6 19.891998 8 ATACCGT 120 0.0047527696 19.799906 6 GTAAGGT 530 0.0 19.727108 4 ACGCAGA 12790 0.0 19.691542 7 CATGGGG 3605 0.0 19.642538 4 >>END_MODULE