FastQCFastQC Report
Thu 26 May 2016
SRR936429_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936429_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1544821
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60940.394479360391916No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58240.3770016073059597No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32870.21277546071680797No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28820.18655883108787363No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17450.11295807087034679No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG13750.025.216662
GTACATG68400.024.6656631
GGTATCA87350.023.7216071
GTACCTG15100.023.6050451
GTATCAA115300.023.3296181
TACATGG71450.023.200142
TATAACG1050.002197331322.6176832
GAGTACT68750.022.52300612-13
ACATGGG70800.021.803773
GTACTTT73150.020.9734414-15
CATGGGG32700.020.9154934
AGTACTT71850.020.42740412-13
TCAACGC134500.019.8810564
TATCAAC135800.019.6913762
ATCAACG136000.019.6630543
CAACGCA136450.019.6323815
TACCGTG1707.9598016E-519.5576447
AACGCAG139400.019.2850636
TAAGGTG6550.018.8538275
AGAGTAC115300.018.39448710-11
Produced by FastQC (version 0.11.5)