##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936429_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1544821 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.899870599894744 31.0 31.0 34.0 30.0 34.0 2 32.042423038008934 31.0 31.0 34.0 30.0 34.0 3 32.14661828134133 33.0 31.0 34.0 30.0 34.0 4 35.890731029679166 37.0 35.0 37.0 35.0 37.0 5 35.669352630498935 37.0 35.0 37.0 33.0 37.0 6 35.772813808201725 37.0 35.0 37.0 33.0 37.0 7 35.7234501602451 37.0 35.0 37.0 33.0 37.0 8 35.696033391570936 37.0 35.0 37.0 33.0 37.0 9 37.33966330079666 39.0 37.0 39.0 34.0 39.0 10-11 37.212651821796825 39.0 37.0 39.0 33.0 39.0 12-13 37.111843378617976 39.0 37.0 39.0 33.0 39.0 14-15 38.22519016766344 40.0 38.0 41.0 33.0 41.0 16-17 38.14403319219508 40.0 37.0 41.0 33.0 41.0 18-19 38.29815590285217 40.0 38.0 41.0 33.5 41.0 20-21 38.33560522545977 40.0 38.0 41.0 34.0 41.0 22-23 38.243669331268805 40.0 38.0 41.0 33.5 41.0 24-25 38.12449532987964 40.0 37.0 41.0 33.0 41.0 26-27 37.907562105900936 40.0 37.0 41.0 33.0 41.0 28-29 37.82138674966226 40.0 37.0 41.0 33.0 41.0 30-31 37.78805635086525 40.0 37.0 41.0 33.0 41.0 32-33 37.63405857377651 40.0 36.5 41.0 33.0 41.0 34-35 37.456001698578675 40.0 36.0 41.0 32.0 41.0 36-37 37.20027983824663 39.0 36.0 41.0 31.5 41.0 38-39 37.0330581989758 39.0 36.0 41.0 31.0 41.0 40-41 36.807156298367254 39.0 35.5 41.0 31.0 41.0 42-43 36.55479728719379 39.0 35.0 41.0 30.0 41.0 44-45 36.29580190844117 39.0 35.0 40.0 30.0 41.0 46-47 35.91384762377 39.0 35.0 40.0 29.5 41.0 48-49 35.534828630631 39.0 35.0 40.0 27.5 41.0 50-51 35.211107953607566 38.0 34.5 40.0 27.0 41.0 52-53 34.93058969291587 38.0 34.0 40.0 26.0 41.0 54-55 34.66961479679523 38.0 34.0 40.0 26.0 41.0 56-57 34.34741241865562 38.0 33.5 40.0 25.0 41.0 58-59 34.004358110098195 37.0 33.0 40.0 24.0 41.0 60-61 33.64756531662891 37.0 33.0 39.5 23.0 41.0 62-63 33.24277472924047 36.0 32.0 39.0 22.0 40.5 64-65 32.8237553088675 36.0 32.0 39.0 22.0 40.0 66-67 32.3684731111242 35.0 31.0 38.5 20.5 40.0 68-69 32.08375404011209 35.0 31.0 38.0 20.0 40.0 70-71 31.648720790305156 35.0 31.0 37.5 18.5 39.5 72-73 31.200004725466577 34.5 30.0 37.0 17.5 39.0 74-75 30.670877726286733 34.0 30.0 36.0 14.0 39.0 76-77 29.260590709214853 32.5 28.5 35.0 12.0 37.0 78-79 30.043873367852974 34.0 29.5 35.0 10.5 37.5 80-81 29.84825199812794 34.0 29.0 35.0 7.0 37.0 82-83 29.503379679587475 34.0 29.0 35.0 7.0 36.5 84-85 29.08910352720477 34.0 29.0 35.0 2.0 36.0 86-87 28.708147416432062 34.0 29.0 35.0 2.0 36.0 88-89 28.30413232342129 33.0 28.5 35.0 2.0 35.0 90-91 27.815301902291594 33.0 27.0 35.0 2.0 35.0 92-93 27.319553203898703 33.0 26.0 35.0 2.0 35.0 94-95 26.72320967930912 32.5 25.0 34.0 2.0 35.0 96-97 25.955962535465275 32.0 24.5 34.0 2.0 35.0 98-99 24.989711429350066 32.0 20.0 34.0 2.0 35.0 100-101 21.722599252599494 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.36189243184930575 1101 2 0.4049488186783705 1101 3 0.3218695311372599 1101 4 0.19920084720971687 1101 5 0.19387741587085827 1101 6 0.21549431019099075 1101 7 0.2469293890155484 1101 8 0.26598938135555983 1101 9 0.39097438907029414 1101 10-11 0.37330729511111116 1101 12-13 0.34986645495102664 1101 14-15 0.5205090185406718 1101 16-17 0.4961618867642059 1101 18-19 0.4659015870043106 1101 20-21 0.3189663897596162 1101 22-23 0.30594197227541287 1101 24-25 0.23374896186204097 1101 26-27 0.3326728175379472 1101 28-29 0.18560539118016095 1101 30-31 0.1926841829057082 1101 32-33 0.3409990823696418 1101 34-35 0.20972519345259144 1101 36-37 0.3092809803944334 1101 38-39 0.3131965323967876 1101 40-41 0.2860173020258543 1101 42-43 0.22685818629861387 1101 44-45 0.2584016077114839 1101 46-47 0.2132342959980491 1101 48-49 0.3356821873787865 1101 50-51 0.3690625325699557 1101 52-53 0.38658150002824954 1101 54-55 0.4484858166758485 1101 56-57 0.4280779294233952 1101 58-59 0.27483370947495445 1101 60-61 0.33613040241586845 1101 62-63 0.3832376374911064 1101 64-65 0.2705298759832715 1101 66-67 0.0853187011280454 1101 68-69 0.3478270577410356 1101 70-71 0.27579566134595623 1101 72-73 0.37459094100527324 1101 74-75 0.36595455187922354 1101 76-77 0.3241011706711845 1101 78-79 0.14135612467281788 1101 80-81 0.24123201544206196 1101 82-83 0.38588809262808965 1101 84-85 0.41976446845388793 1101 86-87 0.49229043873462786 1101 88-89 0.3245556252633328 1101 90-91 0.32544429662335617 1101 92-93 0.310618049985834 1101 94-95 0.36434198513565264 1101 96-97 0.5871711429923572 1101 98-99 0.6936606591968904 1101 100-101 0.8812850903454361 1102 1 0.18508814481563007 1102 2 0.2868286368569599 1102 3 0.26717295803086927 1102 4 0.21261927731161023 1102 5 0.1223221588431116 1102 6 0.27307558530649345 1102 7 0.19525602283734855 1102 8 0.17244125916970887 1102 9 0.29082093052279845 1102 10-11 0.26306749161650345 1102 12-13 0.2568567246740301 1102 14-15 0.4721930177292535 1102 16-17 0.4624656623715282 1102 18-19 0.33912346882039657 1102 20-21 0.21008666167050905 1102 22-23 0.1357295388236608 1102 24-25 0.23871629369959635 1102 26-27 0.18989523621380044 1102 28-29 0.19829038870729931 1102 30-31 0.38388777226681015 1102 32-33 0.30787756146305867 1102 34-35 0.3184089615953596 1102 36-37 0.5247915701986301 1102 38-39 0.4610419535528578 1102 40-41 0.39859774672470394 1102 42-43 0.45530933359123793 1102 44-45 0.426096438475021 1102 46-47 0.4639678817862247 1102 48-49 0.5021238856942389 1102 50-51 0.485030525676585 1102 52-53 0.4185019810448054 1102 54-55 0.5423806607996013 1102 56-57 0.5091642860147232 1102 58-59 0.13546880355718116 1102 60-61 0.3583776018280176 1102 62-63 0.21068440962170598 1102 64-65 0.5003936508796798 1102 66-67 0.503476696018069 1102 68-69 0.34520787456460056 1102 70-71 0.46622307159200815 1102 72-73 0.41405151337378854 1102 74-75 0.08663532807059582 1102 76-77 -0.08875732571696204 1102 78-79 -0.06929591481211261 1102 80-81 0.21943633593754086 1102 82-83 0.2178834643575236 1102 84-85 0.06085225912270076 1102 86-87 0.004135673583199662 1102 88-89 -0.048141194210213456 1102 90-91 0.35861162833834825 1102 92-93 0.3938474024875944 1102 94-95 0.5311078830183007 1102 96-97 0.575774210569886 1102 98-99 0.6000213750344336 1102 100-101 0.6227212270142459 1103 1 0.12207238503329165 1103 2 0.1342825366248519 1103 3 0.05953907658113167 1103 4 0.03306663689417633 1103 5 -0.06361636778906643 1103 6 0.07782428297699795 1103 7 0.017906713902647198 1103 8 0.11333720673557224 1103 9 0.001362376614132188 1103 10-11 0.03286504218541353 1103 12-13 0.07642547811392575 1103 14-15 -0.022959443281756364 1103 16-17 0.16888882006253425 1103 18-19 -0.028696716080887086 1103 20-21 0.1557103042502348 1103 22-23 0.07633786084921468 1103 24-25 0.0027746661045711107 1103 26-27 0.1322219710377297 1103 28-29 0.24115333543694817 1103 30-31 0.06584133692098959 1103 32-33 0.24922202510583702 1103 34-35 0.19732864605816047 1103 36-37 0.1648532239316367 1103 38-39 0.10807627956382504 1103 40-41 0.09838304624325644 1103 42-43 0.1710449673696175 1103 44-45 0.20311788425475896 1103 46-47 0.2117704675997487 1103 48-49 0.1669333202913421 1103 50-51 0.27454510664362175 1103 52-53 0.15909175721521507 1103 54-55 0.12897572268852997 1103 56-57 0.20192148541683252 1103 58-59 0.2773366558003403 1103 60-61 0.29832228784129455 1103 62-63 0.16564254961924973 1103 64-65 0.26007681936263083 1103 66-67 0.41172789378369856 1103 68-69 0.20645266996546496 1103 70-71 0.16317286265902098 1103 72-73 0.13398003842702266 1103 74-75 -0.14141457376220856 1103 76-77 -0.12333695649564902 1103 78-79 -0.07095620328255237 1103 80-81 0.19665789119475363 1103 82-83 0.3341601337616247 1103 84-85 0.1335710365463747 1103 86-87 -0.13253499487353437 1103 88-89 -0.020178898323226946 1103 90-91 -0.14457996652430083 1103 92-93 -0.0027380329340331855 1103 94-95 -0.07283564290529654 1103 96-97 0.0024521685364078394 1103 98-99 0.12382823088880102 1103 100-101 0.48901250240282224 1104 1 0.047965955108416836 1104 2 0.13807570311480788 1104 3 0.07353059639196147 1104 4 0.020373644216107323 1104 5 -0.049291192531917716 1104 6 -0.012488059017194075 1104 7 0.08099693850235923 1104 8 -0.01900163577641223 1104 9 -0.0012954223104841844 1104 10-11 0.04806954773709293 1104 12-13 0.0631824088007491 1104 14-15 0.15439441959436095 1104 16-17 0.05059842713849605 1104 18-19 -0.07084338538079749 1104 20-21 -0.09621393688903623 1104 22-23 0.03507653761925411 1104 24-25 -0.10356223433904432 1104 26-27 0.0864256335004967 1104 28-29 0.07952456277544684 1104 30-31 -0.017208496882211932 1104 32-33 0.049868471528611735 1104 34-35 0.047675814990221 1104 36-37 0.11269513052747726 1104 38-39 0.19942084058389042 1104 40-41 0.15123031274826815 1104 42-43 0.1398487700439759 1104 44-45 0.16073205120044776 1104 46-47 0.01809521911154377 1104 48-49 0.20612374951544865 1104 50-51 0.20031351924482266 1104 52-53 0.23349428067160005 1104 54-55 0.25378883015346076 1104 56-57 0.1602350962994521 1104 58-59 0.26159395843816213 1104 60-61 0.232026975480224 1104 62-63 0.2890297715840404 1104 64-65 0.3131243671711701 1104 66-67 0.07888002863566612 1104 68-69 0.12768181319873406 1104 70-71 0.04648912195009203 1104 72-73 -0.09988381782587652 1104 74-75 0.18514724625341827 1104 76-77 -0.03668103214534568 1104 78-79 0.08955216788902831 1104 80-81 0.289114783762102 1104 82-83 0.35911386364508857 1104 84-85 0.15992248049387214 1104 86-87 0.17450490028176802 1104 88-89 0.1831377307179416 1104 90-91 0.23086487072256645 1104 92-93 0.2854532640289982 1104 94-95 0.1890888711447687 1104 96-97 0.4634662756764918 1104 98-99 0.6080711543561037 1104 100-101 0.930520090936696 1105 1 0.001241262818556521 1105 2 0.08251403925136458 1105 3 0.04873162575739798 1105 4 0.032498404258660685 1105 5 -0.04467925498325798 1105 6 0.020197265393385067 1105 7 0.005632173467496671 1105 8 0.06443372280767079 1105 9 -0.0264738674456666 1105 10-11 0.03825863123336859 1105 12-13 -0.15418917310572056 1105 14-15 0.12050871362915672 1105 16-17 0.2263554445937217 1105 18-19 0.01634645290423009 1105 20-21 0.10695409572733894 1105 22-23 0.09961810746098365 1105 24-25 -0.01598088653595653 1105 26-27 0.04688138921044782 1105 28-29 0.18557520494020707 1105 30-31 0.16962890854632207 1105 32-33 0.2921165830709356 1105 34-35 0.31420464851473184 1105 36-37 0.14943447285091338 1105 38-39 0.31429329911507864 1105 40-41 0.169405417794664 1105 42-43 0.16996261239416555 1105 44-45 0.1349898917914416 1105 46-47 0.2674414650775816 1105 48-49 0.19933703974063377 1105 50-51 0.24106037379643652 1105 52-53 0.2020298419194404 1105 54-55 0.48287434712431576 1105 56-57 0.22323989841999037 1105 58-59 0.1543503637418837 1105 60-61 0.05622894981740245 1105 62-63 0.45604654334663053 1105 64-65 0.3549071247056901 1105 66-67 0.4381034579916019 1105 68-69 0.6253069030899141 1105 70-71 0.3319649360224446 1105 72-73 0.44130862953206673 1105 74-75 0.280133547134799 1105 76-77 0.11070915203499254 1105 78-79 0.3226848680714838 1105 80-81 0.43824083348306786 1105 82-83 0.5378041565863292 1105 84-85 0.40603918153215446 1105 86-87 0.2861934280871168 1105 88-89 0.6680128339813649 1105 90-91 0.6631542282012113 1105 92-93 0.47103382732724697 1105 94-95 0.4830376346581602 1105 96-97 0.3864680170395083 1105 98-99 0.6551103914845804 1105 100-101 0.926879423869849 1106 1 0.0053522275232325 1106 2 0.04554605961749303 1106 3 0.03656759653866004 1106 4 -0.007128557836288962 1106 5 -0.09377800256861946 1106 6 -0.017107804391308434 1106 7 -0.02775958029678094 1106 8 -0.004356334631978598 1106 9 -0.05167567378833127 1106 10-11 -0.009397408884829872 1106 12-13 -0.1365834086722444 1106 14-15 0.06833745648118139 1106 16-17 0.12745178713360872 1106 18-19 0.007551726094369826 1106 20-21 5.871216071682284E-4 1106 22-23 -0.05967074105952719 1106 24-25 -0.10965101152156365 1106 26-27 -0.21341457210414205 1106 28-29 -0.04329077773834911 1106 30-31 0.0820804558348911 1106 32-33 0.060833401758657146 1106 34-35 0.04526331627305069 1106 36-37 -0.12739169864929067 1106 38-39 0.11336230345183651 1106 40-41 0.02074194506250393 1106 42-43 -0.017476461919187614 1106 44-45 -0.053389133141713785 1106 46-47 -0.3187212081048756 1106 48-49 -0.22386877074689693 1106 50-51 -0.23221899526291168 1106 52-53 -0.31532454409036603 1106 54-55 -0.4712308608225655 1106 56-57 -0.1434639739590864 1106 58-59 -0.26311436012563405 1106 60-61 -0.3359904722041094 1106 62-63 -0.18848741512796607 1106 64-65 -0.2217930377628079 1106 66-67 -0.22016356602047438 1106 68-69 -0.21390877064263947 1106 70-71 -0.040307000422952655 1106 72-73 -0.2403618536518053 1106 74-75 -0.07075107464621411 1106 76-77 -0.22018896614484618 1106 78-79 -0.2411150398203894 1106 80-81 -0.011442544838264013 1106 82-83 0.07092470309453702 1106 84-85 -0.20514427994234552 1106 86-87 0.06734900126830468 1106 88-89 -0.18190337321074423 1106 90-91 0.07644370356868535 1106 92-93 -0.055678808116468304 1106 94-95 0.29859399331696324 1106 96-97 0.3114465891077174 1106 98-99 0.6038781254967809 1106 100-101 0.7224291302752022 1107 1 0.01749035527145537 1107 2 0.12721150304177087 1107 3 0.011796612282637398 1107 4 -0.0330258810864521 1107 5 -0.04325179106979249 1107 6 0.015887801061523987 1107 7 0.021987710427737284 1107 8 -0.16348365656547514 1107 9 -0.13123249068132736 1107 10-11 -0.05298307778549116 1107 12-13 -0.04066795188573025 1107 14-15 0.21090994174034705 1107 16-17 -0.05153117047093758 1107 18-19 0.06457165994802949 1107 20-21 0.058943931199820554 1107 22-23 0.01868151885362579 1107 24-25 0.032651947595198294 1107 26-27 0.14548376667620033 1107 28-29 0.055453562864478556 1107 30-31 -0.014751129109185968 1107 32-33 -0.044453873348345496 1107 34-35 -0.02090942554111308 1107 36-37 0.04555478590852147 1107 38-39 0.0729346857239932 1107 40-41 0.016474502125788604 1107 42-43 -0.29327373132930745 1107 44-45 -0.09739815689000153 1107 46-47 0.11196787931284291 1107 48-49 0.10413965593743058 1107 50-51 0.22760498819658892 1107 52-53 0.18433390403873062 1107 54-55 0.1952385227023754 1107 56-57 -0.002310167415629394 1107 58-59 0.09328198083699846 1107 60-61 0.25885437590308413 1107 62-63 0.07739605050240073 1107 64-65 0.05007866648976744 1107 66-67 0.20407905574211327 1107 68-69 0.32131800285241496 1107 70-71 0.05713044800206646 1107 72-73 0.07669312185944932 1107 74-75 0.13393724623525216 1107 76-77 0.04650927016993833 1107 78-79 0.09468780913937636 1107 80-81 0.43767046320385106 1107 82-83 0.16208580998332778 1107 84-85 0.13615990067862782 1107 86-87 0.38688870145876564 1107 88-89 0.12309755888691498 1107 90-91 0.2869002749875733 1107 92-93 0.29094941006477626 1107 94-95 0.6730573853442046 1107 96-97 0.696772829807923 1107 98-99 0.7515051221738211 1107 100-101 1.0782893521274843 1108 1 -0.012972007354978388 1108 2 0.04152026481387594 1108 3 -0.16004734361759532 1108 4 -0.1301462358694252 1108 5 -0.043415617958032726 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589.0 9 1240.0 10 1975.0 11 3055.0 12 4593.0 13 6243.0 14 7955.0 15 10585.0 16 13308.0 17 17130.0 18 15121.0 19 10126.0 20 9466.0 21 9580.0 22 10121.0 23 11210.0 24 13426.0 25 16076.0 26 19681.0 27 24511.0 28 30230.0 29 37510.0 30 46255.0 31 57495.0 32 71570.0 33 91165.0 34 120385.0 35 163310.0 36 231400.0 37 299781.0 38 179311.0 39 9935.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.34677262684008 19.045697915074715 14.917032976995625 22.690496481089582 2 16.0285205821938 20.229894433190974 39.42030505422057 24.321279930394656 3 19.90537065856014 24.08180149200784 30.002900119370985 26.009927730061033 4 13.287433495186797 16.80423455783969 36.44279275226193 33.46553919471159 5 13.786706041596133 37.294302783454505 33.85367880906806 15.065312365881303 6 32.367976271623114 35.802946238939626 16.92168069098152 14.907396798455737 7 28.841335015513124 32.75602804467314 20.106018755571036 18.296618184242703 8 27.03083399306457 34.16337556260564 19.236403440916455 19.56938700341334 9 26.474714055169922 16.545862838403384 20.22448344144936 36.75493966497733 10-11 25.373337704947748 26.383361885844547 26.654074000470608 21.5892264087371 12-13 26.455951898151582 23.70385786430719 27.51686215513862 22.323328082402607 14-15 23.054266180341777 25.1018993923744 26.245036925886378 25.598797501397446 16-17 22.175538150715216 28.344665036662153 27.067752645844294 22.412044166778333 18-19 22.001934204674846 26.641630324807856 29.74124510218336 21.615190368333938 20-21 22.744512147362055 25.512794038921015 29.998459368431686 21.744234445285247 22-23 22.327823878705257 25.57009034832363 29.98613411841929 22.11595165455182 24-25 22.370187152404778 25.734191993127958 30.07783155280211 21.817789301665155 26-27 22.22576106408659 25.896133480799467 29.956436640866567 21.92166881424738 28-29 21.91072189214279 26.223858161794883 30.00085450941449 21.864565436647837 30-31 22.086940985812788 25.798635622001846 29.94164641332102 22.17277697886434 32-33 21.991353004309904 25.976266398414303 29.895735020526743 22.136645576749046 34-35 22.588212140937443 25.865548606703115 29.59096575094379 21.955273501415647 36-37 22.562062950361618 25.72430917712392 29.60010979751874 22.11351807499572 38-39 22.276123497399002 25.916838679960758 29.83113511294937 21.975902709690867 40-41 22.3985421990332 25.927333282625337 29.63064618710927 22.043478331232187 42-43 22.23777604438072 26.084029479258035 29.253341014943533 22.424853461417708 44-45 22.518937702048547 26.07411823651186 29.02035341746062 22.38659064397897 46-47 22.748702114162167 26.46447482554602 28.449819396434535 22.337003663857278 48-49 22.93199888918594 26.604644057241877 28.155996874702634 22.307360178869544 50-51 22.73138769339421 26.76322473167317 28.123040811960347 22.382346762972272 52-53 23.067596378641742 26.852473119950126 27.852842428320677 22.227088073087454 54-55 22.50297212241422 26.785514880356093 28.003720938663097 22.70779205856659 56-57 22.789373807900088 27.01666973609299 27.73760191817028 22.45635453783664 58-59 23.104320050025617 26.613654702184768 27.75997801669268 22.522047231096927 60-61 22.767714379302443 26.557100705480845 28.400070690501188 22.275114224715523 62-63 22.725669056048513 26.708446862957203 28.24484334368452 22.321040737309765 64-65 22.534917211517268 26.800693602177006 28.07043098305517 22.593958203250555 66-67 22.478695940382295 26.95118903970543 27.825005049275752 22.74510997063652 68-69 22.635781056859074 26.67332567094701 28.07828710385425 22.61260616833967 70-71 22.620193536985838 26.8602640694294 27.868212563138385 22.651329830446375 72-73 22.717448016126298 26.70503610162053 27.899846323745507 22.67766955850767 74-75 22.562951252875106 27.14414909560071 27.567460140206002 22.725439511318182 76-77 22.709072177428098 26.876225697646305 27.660672191281172 22.754029933644425 78-79 22.72459506890674 26.914703751823087 27.641349612790002 22.719351566480167 80-81 22.746630112688322 27.11815361410198 27.492842681409098 22.642373591800602 82-83 22.718412788631408 27.050610876732566 27.54282740150976 22.688148933126264 84-85 22.612248808535817 27.075012073244782 27.439378069290715 22.873361048928686 86-87 22.667960931249045 27.061706513098443 27.337217853342 22.933114702310515 88-89 22.54793873164668 27.023687754097935 27.243895175699823 23.184478338555557 90-91 22.548097372375338 26.828677732552908 27.539755552635036 23.083469342436718 92-93 22.58597938184371 26.713494628928537 27.741457084994952 22.959068904232804 94-95 22.3640255323639 26.724569025647266 27.717134655351604 23.19427078663723 96-97 22.30897551919275 26.58528406876739 27.789321867786292 23.316418544253565 98-99 22.2762180870467 26.65289329760414 27.425112565410288 23.645776049938874 100-101 21.951972655681036 26.703630696328517 27.56697707388598 23.777419574104464 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 192.0 1 164.5 2 214.0 3 286.5 4 233.5 5 206.5 6 356.0 7 706.0 8 1113.5 9 1391.5 10 1415.5 11 1289.0 12 1322.0 13 1547.5 14 1775.0 15 1956.5 16 2153.5 17 2363.5 18 2558.5 19 2846.0 20 3100.5 21 3392.0 22 3914.5 23 4581.0 24 5483.5 25 6568.5 26 7983.0 27 9985.0 28 12114.5 29 14552.5 30 17620.5 31 20825.0 32 24105.5 33 26632.0 34 29262.5 35 31850.5 36 34463.0 37 37524.0 38 39717.0 39 41080.0 40 42504.5 41 45366.5 42 48289.5 43 50693.5 44 52928.5 45 54987.5 46 56374.0 47 59172.0 48 66850.5 49 66857.0 50 60103.0 51 56608.5 52 53598.5 53 52240.0 54 50804.5 55 47724.0 56 43541.5 57 39321.5 58 35984.5 59 31925.0 60 26958.5 61 22243.5 62 18436.5 63 14662.0 64 10749.0 65 8174.5 66 6465.5 67 5296.0 68 4181.5 69 2990.0 70 2155.5 71 1651.5 72 1382.0 73 1189.5 74 979.0 75 811.5 76 626.5 77 419.5 78 266.5 79 177.0 80 124.0 81 84.0 82 64.5 83 46.0 84 25.0 85 11.0 86 7.5 87 5.5 88 4.0 89 3.0 90 2.5 91 2.0 92 1.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.038386324370266844 2 0.007250030909729995 3 0.003689747873701872 4 5.178593506949996E-4 5 9.062538637162493E-4 6 2.589296753474998E-4 7 0.0 8 0.0 9 0.0019419725651062484 10-11 4.207607224396871E-4 12-13 0.001003352491971562 14-15 0.0024274657063828105 16-17 0.002589296753474998 18-19 0.0 20-21 0.0 22-23 0.0014241132144112488 24-25 6.79690397787187E-4 26-27 0.0038515789207940595 28-29 0.004854931412765621 30-31 9.386200731346868E-4 32-33 4.5312693185812467E-4 34-35 0.007864988888680306 36-37 0.010324820804481554 38-39 0.008059186145190932 40-41 0.001844873936850936 42-43 3.883945130212497E-4 44-45 0.001229915957900624 46-47 6.473241883687495E-5 48-49 5.178593506949996E-4 50-51 0.0010680849108084367 52-53 0.0026863953817303104 54-55 0.00320425473242531 56-57 0.001003352491971562 58-59 8.091552354609369E-4 60-61 0.0038192127113756223 62-63 0.0045312693185812465 64-65 0.008512313077049057 66-67 0.0029129588476593726 68-69 0.0029453250570778103 70-71 0.0 72-73 9.709862825531242E-4 74-75 0.006084847370666246 76-77 0.0024274657063828105 78-79 0.003366085779517497 80-81 0.004757832784510308 82-83 0.0047901989939287465 84-85 0.005372790763460621 86-87 0.003689747873701872 88-89 0.00912727105599937 90-91 0.01255808925435374 92-93 0.0011328173296453116 94-95 0.0023627332875459355 96-97 0.001229915957900624 98-99 0.003398451988935935 100-101 0.004919663831602496 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1544821.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.20917704797546 #Duplication Level Percentage of deduplicated Percentage of total 1 81.4212060294967 51.46567427378144 2 11.407686603169752 14.421409644151492 3 3.201658064416984 6.071225143224347 4 1.3472315459961746 3.4062958926195965 5 0.6701403414244497 2.117950974404439 6 0.42840647167258744 1.6247532309870638 7 0.27742967117675726 1.2275270842641286 8 0.20287283189110508 1.025873979938322 9 0.1499932954269344 0.8532857489985347 >10 0.8035458753601159 9.318271696752754 >50 0.05663072459589737 2.469058216665627 >100 0.030432135693121233 3.626308382278223 >500 0.0020491923551722926 0.9301388616755061 >1k 5.122980887930732E-4 0.6703709342155106 >5k 2.0491923551722925E-4 0.7718559360429385 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6094 0.394479360391916 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5824 0.3770016073059597 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3287 0.21277546071680797 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2882 0.18655883108787363 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1745 0.11295807087034679 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 6.473241883687495E-5 0.0 0.0 0.0 0.0 8 6.473241883687495E-5 0.0 0.0 0.0 6.473241883687495E-5 9 6.473241883687495E-5 0.0 0.0 0.0 1.294648376737499E-4 10-11 6.473241883687495E-5 0.0 0.0 0.0 1.294648376737499E-4 12-13 6.473241883687495E-5 0.0 0.0 0.0 1.6183104709218738E-4 14-15 1.294648376737499E-4 0.0 0.0 0.0 1.9419725651062485E-4 16-17 1.294648376737499E-4 0.0 0.0 0.0 1.9419725651062485E-4 18-19 1.6183104709218738E-4 0.0 0.0 0.0 2.589296753474998E-4 20-21 1.9419725651062485E-4 0.0 0.0 6.473241883687495E-5 4.5312693185812467E-4 22-23 1.9419725651062485E-4 0.0 0.0 1.294648376737499E-4 4.5312693185812467E-4 24-25 1.9419725651062485E-4 0.0 0.0 2.589296753474998E-4 5.178593506949996E-4 26-27 1.9419725651062485E-4 0.0 0.0 3.5602830360281226E-4 5.178593506949996E-4 28-29 1.9419725651062485E-4 0.0 0.0 0.0010033524919715617 5.178593506949996E-4 30-31 1.9419725651062485E-4 0.0 0.0 0.0017801415180140612 5.178593506949996E-4 32-33 1.9419725651062485E-4 0.0 0.0 0.0026216629628934354 5.178593506949996E-4 34-35 1.9419725651062485E-4 0.0 0.0 0.004304705852652184 5.178593506949996E-4 36-37 1.9419725651062485E-4 0.0 0.0 0.007185298490893119 5.178593506949996E-4 38-39 1.9419725651062485E-4 0.0 0.0 0.012040229903658741 5.178593506949996E-4 40-41 1.9419725651062485E-4 0.0 0.0 0.016571499222239987 5.178593506949996E-4 42-43 2.265634659290623E-4 0.0 0.0 0.023141839734182795 5.502255601134371E-4 44-45 3.2366209418437477E-4 0.0 0.0 0.029712180246125604 6.14957978950312E-4 46-47 3.2366209418437477E-4 0.0 0.0 0.04210843845338716 7.120566072056245E-4 48-49 3.2366209418437477E-4 0.0 0.0 0.05634957059749965 7.120566072056245E-4 50-51 3.2366209418437477E-4 0.0 0.0 0.06353486908839276 7.120566072056245E-4 52-53 3.2366209418437477E-4 0.0 0.0 0.07486304238484588 7.120566072056245E-4 54-55 3.2366209418437477E-4 0.0 0.0 0.095512683993809 7.120566072056245E-4 56-57 3.2366209418437477E-4 0.0 0.0 0.12690790712969335 7.120566072056245E-4 58-59 3.2366209418437477E-4 0.0 0.0 0.16034220145893924 7.120566072056245E-4 60-61 3.2366209418437477E-4 0.0 0.0 0.1996671459023408 7.120566072056245E-4 62-63 3.2366209418437477E-4 0.0 0.0 0.23873316067039482 7.120566072056245E-4 64-65 3.2366209418437477E-4 0.0 0.0 0.281068162589711 7.767890260424994E-4 66-67 3.2366209418437477E-4 0.0 0.0 0.346609736662047 7.767890260424994E-4 68-69 3.2366209418437477E-4 0.0 0.0 0.4280431195588356 7.767890260424994E-4 70-71 3.5602830360281226E-4 0.0 0.0 0.4714462063889603 7.767890260424994E-4 72-73 4.207607224396872E-4 0.0 0.0 0.5307411020435377 7.767890260424994E-4 74-75 4.5312693185812467E-4 0.0 0.0 0.5987748742410933 7.767890260424994E-4 76-77 4.5312693185812467E-4 0.0 0.0 0.6637985889627342 7.767890260424994E-4 78-79 4.5312693185812467E-4 0.0 0.0 0.7393089555359488 7.767890260424994E-4 80-81 5.825917695318746E-4 0.0 0.0 0.8623329175354297 7.767890260424994E-4 82-83 5.825917695318746E-4 0.0 0.0 0.9984975605587961 7.767890260424994E-4 84-85 5.825917695318746E-4 0.0 0.0 1.1936981695613926 7.767890260424994E-4 86-87 5.825917695318746E-4 0.0 0.0 1.4789739393755004 8.091552354609369E-4 88-89 5.825917695318746E-4 0.0 0.0 1.7855143087775218 8.415214448793744E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1375 0.0 25.21666 2 GTACATG 6840 0.0 24.665663 1 GGTATCA 8735 0.0 23.721607 1 GTACCTG 1510 0.0 23.605045 1 GTATCAA 11530 0.0 23.329618 1 TACATGG 7145 0.0 23.20014 2 TATAACG 105 0.0021973313 22.617683 2 GAGTACT 6875 0.0 22.523006 12-13 ACATGGG 7080 0.0 21.80377 3 GTACTTT 7315 0.0 20.97344 14-15 CATGGGG 3270 0.0 20.915493 4 AGTACTT 7185 0.0 20.427404 12-13 TCAACGC 13450 0.0 19.881056 4 TATCAAC 13580 0.0 19.691376 2 ATCAACG 13600 0.0 19.663054 3 CAACGCA 13645 0.0 19.632381 5 TACCGTG 170 7.9598016E-5 19.557644 7 AACGCAG 13940 0.0 19.285063 6 TAAGGTG 655 0.0 18.853827 5 AGAGTAC 11530 0.0 18.394487 10-11 >>END_MODULE