FastQCFastQC Report
Thu 26 May 2016
SRR936428_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936428_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1635397
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48270.29515768953960414No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40150.24550613704195373No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25570.15635347258188684No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22260.1361137387435589No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATA650.00582946429.2464732
GTATCAA103400.026.9809821
GTACATG76300.025.1027151
GTACCTG14550.024.1717951
TACATGG78150.024.0212172
TACCTGG14850.023.6827532
ACATGGG79900.022.781323
TCAACGC123050.022.6716634
TATCAAC122200.022.5571212
ATCAACG123100.022.430813
CAACGCA124700.022.2580225
GAGTACT54400.022.01751912-13
GTACACG3251.8189894E-1221.9355261
AACGCAG127000.021.929776
GGTATCA75650.021.2347891
AGTACTT55950.020.81290612-13
GTACTTT58200.020.1267814-15
CTAGTAC3800.020.0107463
ACGCAGA138500.019.9029927
TATCCCG1200.004748721619.8029065
Produced by FastQC (version 0.11.5)