##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936428_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1635397 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.2836051429714 31.0 31.0 34.0 28.0 34.0 2 31.342165235719524 31.0 31.0 34.0 28.0 34.0 3 31.283821604173177 31.0 31.0 34.0 27.0 34.0 4 34.95147539099069 37.0 35.0 37.0 32.0 37.0 5 34.98236574972316 37.0 35.0 37.0 32.0 37.0 6 35.03797793440981 37.0 35.0 37.0 32.0 37.0 7 34.90136339983503 37.0 35.0 37.0 32.0 37.0 8 34.88382820807425 37.0 35.0 37.0 32.0 37.0 9 36.359977424441894 39.0 37.0 39.0 32.0 39.0 10-11 36.237904618878474 39.0 35.0 39.0 32.0 39.0 12-13 36.14045549796165 39.0 35.0 39.0 31.5 39.0 14-15 37.12099019381838 39.5 36.5 41.0 31.0 41.0 16-17 37.03137097597708 39.0 36.0 41.0 31.0 41.0 18-19 36.903115573772 39.0 36.0 41.0 31.0 41.0 20-21 36.892218831268494 39.0 36.0 41.0 30.5 41.0 22-23 36.88841363901243 39.0 36.0 41.0 30.5 41.0 24-25 37.00485539596808 39.0 36.0 41.0 31.0 41.0 26-27 36.93304592095986 39.0 36.0 41.0 30.0 41.0 28-29 36.81457101853556 39.0 36.0 41.0 30.0 41.0 30-31 36.619638839988085 39.0 36.0 41.0 30.0 41.0 32-33 36.4586604965033 39.0 35.0 41.0 30.0 41.0 34-35 36.273255668195546 39.0 35.0 40.0 30.0 41.0 36-37 36.06832377704007 38.5 35.0 40.0 29.5 41.0 38-39 35.87614750424515 38.0 35.0 40.0 28.5 41.0 40-41 35.64353395536374 38.0 35.0 40.0 27.5 41.0 42-43 35.37266883820871 38.0 34.5 40.0 26.5 41.0 44-45 35.22322744874792 38.0 34.0 40.0 26.0 41.0 46-47 35.08468433047144 38.0 34.0 40.0 25.5 41.0 48-49 34.84841509431655 38.0 34.0 40.0 24.0 41.0 50-51 33.86681857677371 37.5 33.0 39.5 23.5 40.5 52-53 33.970638933543356 37.5 33.0 39.5 23.5 40.5 54-55 34.413630146074624 38.0 34.0 40.0 24.0 41.0 56-57 34.270885907213966 38.0 34.0 40.0 23.0 41.0 58-59 34.04785229519193 38.0 33.0 40.0 23.0 41.0 60-61 33.74730325419455 37.0 33.0 40.0 22.0 41.0 62-63 33.40560579480089 37.0 33.0 40.0 21.5 41.0 64-65 33.04875513407448 36.0 32.0 39.0 20.5 41.0 66-67 32.69232486056902 36.0 32.0 39.0 19.5 41.0 68-69 32.26041719533544 35.0 31.0 39.0 18.0 40.0 70-71 31.861572755728425 35.0 31.0 38.0 17.5 40.0 72-73 31.44169366826526 35.0 31.0 37.5 13.5 39.5 74-75 31.028600700624985 35.0 30.5 37.0 11.0 39.0 76-77 30.568085302834724 34.5 30.0 36.0 7.5 39.0 78-79 30.1076209629833 34.0 30.0 36.0 7.0 38.5 80-81 29.66637764408275 34.0 29.0 35.5 3.5 37.0 82-83 29.282336337904496 34.0 29.0 35.0 2.0 37.0 84-85 28.913190191739375 34.0 29.0 35.0 2.0 36.0 86-87 28.559861917320383 34.0 29.0 35.0 2.0 36.0 88-89 28.2061826577889 34.0 27.5 35.0 2.0 36.0 90-91 27.854668927483665 34.0 27.0 35.0 2.0 35.0 92-93 27.43425357879463 33.0 26.5 35.0 2.0 35.0 94-95 26.95179060497237 33.0 25.5 35.0 2.0 35.0 96-97 26.34629328536129 33.0 25.0 35.0 2.0 35.0 98-99 25.590008725710028 33.0 22.0 35.0 2.0 35.0 100-101 23.03492057280281 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5980162629516315 1101 2 0.6042638465473331 1101 3 0.5451143494881059 1101 4 0.5868592969443682 1101 5 0.4215803828441622 1101 6 0.5145986336298733 1101 7 0.5687405316727947 1101 8 0.7102582095811698 1101 9 0.7675718886862484 1101 10-11 0.8938107937587958 1101 12-13 0.9267909367140632 1101 14-15 1.0516705319264972 1101 16-17 1.0456391553975166 1101 18-19 1.2273277695369842 1101 20-21 1.236153647802439 1101 22-23 1.07239729569163 1101 24-25 0.9042748386912081 1101 26-27 0.8335554853712424 1101 28-29 0.9128456304137273 1101 30-31 0.6652424242442123 1101 32-33 0.604926971658962 1101 34-35 0.7443788989846638 1101 36-37 0.8495449361401768 1101 38-39 0.7455979127048096 1101 40-41 0.7259502405360507 1101 42-43 0.8350623015018073 1101 44-45 0.7086101224877055 1101 46-47 0.7372156625974924 1101 48-49 0.8659485245140814 1101 50-51 0.8039793143699754 1101 52-53 0.6200420149271082 1101 54-55 0.6380810378822446 1101 56-57 0.6935738235779354 1101 58-59 0.8510357934783741 1101 60-61 0.9121403869415659 1101 62-63 0.8953427810665815 1101 64-65 0.7811697634951074 1101 66-67 0.702616004896818 1101 68-69 0.8265158527666685 1101 70-71 0.7051395487573515 1101 72-73 0.6854457619447523 1101 74-75 0.421192059520223 1101 76-77 0.6251449318388893 1101 78-79 0.7080879522441315 1101 80-81 0.8159080136364665 1101 82-83 0.7359851993607087 1101 84-85 0.8711159027714395 1101 86-87 0.6148682425512106 1101 88-89 0.7359680334643315 1101 90-91 0.7777061169750645 1101 92-93 0.9653494398968547 1101 94-95 1.111767202797072 1101 96-97 1.0369310628684438 1101 98-99 0.902630889105378 1101 100-101 1.2315168650985235 1102 1 0.3673108228980304 1102 2 0.4356255070184645 1102 3 0.48302241094605236 1102 4 0.3795894576556549 1102 5 0.523725487162281 1102 6 0.47092843158191755 1102 7 0.48092354811959837 1102 8 0.4398948387020667 1102 9 0.6533710317125596 1102 10-11 0.6517556216918834 1102 12-13 0.5431898029827593 1102 14-15 0.8083180391675597 1102 16-17 0.5938530070009165 1102 18-19 0.6619985059127913 1102 20-21 0.7015171878905306 1102 22-23 0.5393627326591144 1102 24-25 0.6033524789181186 1102 26-27 0.5685596100344981 1102 28-29 0.5035245745123547 1102 30-31 0.447379636282335 1102 32-33 0.4752802831410534 1102 34-35 0.03386133369406252 1102 36-37 0.2127467614860663 1102 38-39 -0.02832257272940808 1102 40-41 0.22789940394505237 1102 42-43 0.09379744409842061 1102 44-45 0.2703443935803804 1102 46-47 0.5418201032003225 1102 48-49 0.5709557756627532 1102 50-51 0.3454851936839134 1102 52-53 0.20831617323656104 1102 54-55 0.35108333633026945 1102 56-57 0.38569666371827793 1102 58-59 0.39253965470339836 1102 60-61 0.42132921144824564 1102 62-63 0.5196612491570249 1102 64-65 0.5224782426664092 1102 66-67 0.4301932519068572 1102 68-69 0.522510784569242 1102 70-71 0.3727058173637374 1102 72-73 0.5818380088818387 1102 74-75 0.40314460089958004 1102 76-77 0.6040767045995921 1102 78-79 0.39010389266269385 1102 80-81 0.6496972798251832 1102 82-83 0.4361516761717823 1102 84-85 0.5482682489239856 1102 86-87 0.33630335835380976 1102 88-89 0.2770103732800777 1102 90-91 0.4117516183599399 1102 92-93 0.4192111151155622 1102 94-95 0.4834535396210775 1102 96-97 0.3684302638604784 1102 98-99 0.32971004072564014 1102 100-101 0.8949193505768207 1103 1 0.4003827744281203 1103 2 0.45150787986420227 1103 3 0.4614069134924925 1103 4 0.40791521904962735 1103 5 0.47370319580023335 1103 6 0.43168287100610314 1103 7 0.4811838613622115 1103 8 0.2522437189989333 1103 9 0.3961346500725682 1103 10-11 0.4565125271165513 1103 12-13 0.32521397144965647 1103 14-15 0.6365053625953294 1103 16-17 0.8628162479215646 1103 18-19 0.7755213484740295 1103 20-21 0.6981685860130753 1103 22-23 0.5735626092997279 1103 24-25 0.5100594184476819 1103 26-27 0.5793890267962922 1103 28-29 0.5129206359165934 1103 30-31 0.44064117421415716 1103 32-33 0.48248950553166736 1103 34-35 0.6111280624559754 1103 36-37 0.4760896703099462 1103 38-39 0.3123967407322681 1103 40-41 0.54667552802632 1103 42-43 0.7740780184378835 1103 44-45 0.6034165232039115 1103 46-47 0.7764141303025838 1103 48-49 0.790727531111969 1103 50-51 0.8137836711631081 1103 52-53 0.849446021756215 1103 54-55 0.8027324518885735 1103 56-57 0.8541114955772997 1103 58-59 0.8274701003109897 1103 60-61 0.8466210917528301 1103 62-63 0.8925178204190374 1103 64-65 0.9138948216220584 1103 66-67 1.0128673426953299 1103 68-69 0.9579942103033829 1103 70-71 0.8956276301887272 1103 72-73 0.9599524920932687 1103 74-75 0.8177302457373372 1103 76-77 0.7930791082673672 1103 78-79 0.8833608298473585 1103 80-81 0.8840168946209488 1103 82-83 0.5842516566408698 1103 84-85 0.7445466600218218 1103 86-87 0.6365190295078378 1103 88-89 0.5597559685841098 1103 90-91 0.7666065048580215 1103 92-93 0.6172795136815523 1103 94-95 0.710141405343375 1103 96-97 0.9000575807819082 1103 98-99 0.9831008804302606 1103 100-101 1.3737369170012208 1104 1 0.2826868184098714 1104 2 0.32002597738970806 1104 3 0.37367624386871867 1104 4 0.5164465749627141 1104 5 0.3598071677054051 1104 6 0.36159129442069826 1104 7 0.3617487542856921 1104 8 0.5179505987253492 1104 9 0.5082617362295281 1104 10-11 0.5019825201807677 1104 12-13 0.5893128667575596 1104 14-15 0.6392270342165389 1104 16-17 0.6122601997077766 1104 18-19 0.6236695705369897 1104 20-21 0.7369636866570701 1104 22-23 0.4591945548408347 1104 24-25 0.5422853409092454 1104 26-27 0.1874479224975545 1104 28-29 0.09642085602202854 1104 30-31 0.18657934685895583 1104 32-33 0.23645444022023554 1104 34-35 0.30287141894520175 1104 36-37 0.19802304633389411 1104 38-39 0.201362227853636 1104 40-41 0.13523556995335895 1104 42-43 0.2349673658556526 1104 44-45 0.30626117620379745 1104 46-47 0.5294380491973456 1104 48-49 0.5108696943463258 1104 50-51 0.4027606407885571 1104 52-53 0.41839513257077243 1104 54-55 0.4491329327075846 1104 56-57 0.480072540676062 1104 58-59 0.5731802136493798 1104 60-61 0.5632540050636763 1104 62-63 0.561031955468323 1104 64-65 0.45852546388449866 1104 66-67 0.48366288065788154 1104 68-69 0.5201216435385518 1104 70-71 0.4595938548512919 1104 72-73 0.5740051392298433 1104 74-75 0.59555492727862 1104 76-77 0.5811611078080681 1104 78-79 0.4629003091259243 1104 80-81 0.6033509440923197 1104 82-83 0.5799329914111055 1104 84-85 0.4445697117995273 1104 86-87 0.26724343379147797 1104 88-89 0.3417509394208622 1104 90-91 0.3853530955376989 1104 92-93 0.40383943066044736 1104 94-95 0.5697137066512212 1104 96-97 0.5499540068597426 1104 98-99 0.2139444771554615 1104 100-101 0.8546805879433101 1105 1 0.301274620654322 1105 2 0.237907224914629 1105 3 0.44400778327481305 1105 4 0.4426589003539547 1105 5 0.3957212301386335 1105 6 0.4073783194549563 1105 7 0.49094655826804257 1105 8 0.47704877279421964 1105 9 0.3440330632133666 1105 10-11 0.5206717472155944 1105 12-13 0.4593160576498434 1105 14-15 0.8039314920639953 1105 16-17 0.6979312415056143 1105 18-19 0.43861192080009204 1105 20-21 0.4156310047975822 1105 22-23 0.4456294164786243 1105 24-25 0.27679227682644125 1105 26-27 0.3078174962000446 1105 28-29 0.43820616025366377 1105 30-31 0.22048115284700742 1105 32-33 0.2696840220708978 1105 34-35 0.28942850315342383 1105 36-37 0.23959510209473933 1105 38-39 -0.007886373994232088 1105 40-41 0.15564973067746735 1105 42-43 0.12154937890981898 1105 44-45 0.22095210124916065 1105 46-47 0.7315161177958345 1105 48-49 0.6780352703166272 1105 50-51 0.8200980121200985 1105 52-53 0.8142770296701869 1105 54-55 0.6125372444007837 1105 56-57 0.5056318727539306 1105 58-59 0.5870199966687011 1105 60-61 0.6063515637461805 1105 62-63 0.6585289568842825 1105 64-65 0.5909094592804962 1105 66-67 0.6078655137138185 1105 68-69 0.6631763246060203 1105 70-71 0.7993451077038642 1105 72-73 0.7545842861789787 1105 74-75 0.6677203750434266 1105 76-77 0.6985785363477497 1105 78-79 0.5448241892509351 1105 80-81 0.5370393591590314 1105 82-83 0.47548967390877905 1105 84-85 0.41874698865786186 1105 86-87 0.4987627543593405 1105 88-89 0.4398862617437196 1105 90-91 0.5339379917909675 1105 92-93 0.7306476266951947 1105 94-95 0.7098386072919638 1105 96-97 0.7707979408880448 1105 98-99 1.10771839104126 1105 100-101 1.4565096529833816 1106 1 0.267513254553954 1106 2 0.21526147553344543 1106 3 0.27825462864516126 1106 4 0.3787519186326449 1106 5 0.3399091365233602 1106 6 0.2287636249774252 1106 7 0.39596242645647806 1106 8 0.48746095145283874 1106 9 0.5106214449397442 1106 10-11 0.5530338404084745 1106 12-13 0.5195820856465687 1106 14-15 0.39267310388896703 1106 16-17 0.29382142294082314 1106 18-19 0.23171679164401837 1106 20-21 0.1314289339074719 1106 22-23 0.01279944404731026 1106 24-25 -0.2358020241425436 1106 26-27 -0.32853825534945713 1106 28-29 -0.13754043308423292 1106 30-31 -0.19017686305215875 1106 32-33 0.009713674604029165 1106 34-35 0.17904997575510606 1106 36-37 -0.1305539772124078 1106 38-39 0.002233925741343512 1106 40-41 -0.04183797042048809 1106 42-43 -0.004518379803606365 1106 44-45 0.01668134799597709 1106 46-47 0.008262111324654597 1106 48-49 -0.1329422213550373 1106 50-51 -0.3389274248534804 1106 52-53 -0.0856732678407397 1106 54-55 0.049189098880908944 1106 56-57 0.2643942948561886 1106 58-59 0.09440426590131779 1106 60-61 0.23650055857318364 1106 62-63 0.3344069168575885 1106 64-65 0.23756122928046608 1106 66-67 0.12774650934056098 1106 68-69 0.013932703561451376 1106 70-71 -0.1540273399395602 1106 72-73 -0.04500682988829752 1106 74-75 0.17432672288200024 1106 76-77 0.15393978755511029 1106 78-79 0.29511663137499156 1106 80-81 0.34810823019444825 1106 82-83 0.38489034355477614 1106 84-85 0.6112624696456059 1106 86-87 0.608159340798025 1106 88-89 0.6374112749085405 1106 90-91 0.6785312621021085 1106 92-93 0.7233768424659459 1106 94-95 0.831374850684913 1106 96-97 0.9244729682736939 1106 98-99 0.9670582349782606 1106 100-101 1.3248718362884162 1107 1 0.2682599521154998 1107 2 0.2878639064191262 1107 3 0.3805805434098488 1107 4 0.3769581863456466 1107 5 0.4164961830857834 1107 6 0.22677305551088267 1107 7 0.39169940384507385 1107 8 0.4285469952421579 1107 9 0.5273360238084734 1107 10-11 0.5367503777536484 1107 12-13 0.3544330175877022 1107 14-15 0.2688972853899614 1107 16-17 0.5463568382552353 1107 18-19 0.6578289016503334 1107 20-21 0.5066491388160514 1107 22-23 0.4629851957634159 1107 24-25 0.4237644633648472 1107 26-27 0.27949018762298294 1107 28-29 0.4493016700675483 1107 30-31 0.3542158046559294 1107 32-33 0.39263834346424886 1107 34-35 0.16104592615106128 1107 36-37 0.33039216241201075 1107 38-39 0.5196985451943661 1107 40-41 0.4892185772105009 1107 42-43 0.41558337646008425 1107 44-45 0.41691003749628663 1107 46-47 0.5093400355803155 1107 48-49 0.43108548989658857 1107 50-51 0.5707776643771467 1107 52-53 0.6927895621590636 1107 54-55 0.8332599164050833 1107 56-57 0.702503978630304 1107 58-59 0.7704887625397063 1107 60-61 0.6455652583657283 1107 62-63 0.6374388245388118 1107 64-65 0.6090121091774279 1107 66-67 0.6690491542599233 1107 68-69 0.4678741407563507 1107 70-71 0.4430295698076492 1107 72-73 0.647731001442029 1107 74-75 0.5333882267447976 1107 76-77 0.533167608136953 1107 78-79 0.8028709732260815 1107 80-81 0.841197934727397 1107 82-83 0.8012148852731578 1107 84-85 0.7723678173854935 1107 86-87 0.960736584013091 1107 88-89 0.8942285674826742 1107 90-91 0.992619615786424 1107 92-93 1.0082011773180852 1107 94-95 0.8930041436944371 1107 96-97 1.2716045611491502 1107 98-99 1.5057630837786888 1107 100-101 1.8103381117411885 1108 1 0.14760980990583406 1108 2 0.20716232772399934 1108 3 0.30651321487718164 1108 4 0.35900411272236 1108 5 0.23389308314064294 1108 6 0.3462123934174457 1108 7 0.3390430351134839 1108 8 0.42593087660589646 1108 9 0.07711050772411454 1108 10-11 0.30285811925283923 1108 12-13 0.2484279399680176 1108 14-15 0.6810098403933864 1108 16-17 0.7244603462037276 1108 18-19 0.7730433551486726 1108 20-21 0.6704371762566126 1108 22-23 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-6.183177958313479 2316 98-99 -7.945208134488009 2316 100-101 -8.31848119017829 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4337.0 3 1660.0 4 1681.0 5 1860.0 6 2025.0 7 2510.0 8 3006.0 9 3779.0 10 4665.0 11 5972.0 12 7236.0 13 8294.0 14 9667.0 15 11018.0 16 13245.0 17 16334.0 18 13425.0 19 10856.0 20 10935.0 21 11986.0 22 13189.0 23 15081.0 24 17037.0 25 19767.0 26 22935.0 27 27366.0 28 32577.0 29 38909.0 30 47114.0 31 57410.0 32 71153.0 33 90828.0 34 117977.0 35 160265.0 36 221850.0 37 286505.0 38 219703.0 39 31224.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.55576477413267 18.385396937684618 14.18155181709088 22.877286471091836 2 15.677806961634309 19.809205270710514 40.14610550473867 24.366882262916505 3 19.772934652050854 24.174068755641773 29.39030923648604 26.66268735582133 4 12.89934306975595 16.183443841057255 36.819469257120204 34.097743832066584 5 14.00791585292979 37.24512170730917 33.71681161530007 15.030150824460978 6 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52-53 23.041518753743365 27.18364112368063 27.213481036826913 22.56135908574909 54-55 22.614747647813093 27.001613679403054 27.443036627893115 22.94060204489074 56-57 22.812683544590197 27.291744823603043 27.181216774702865 22.714354857103896 58-59 23.069850861631956 26.973963005564023 27.160041997716668 22.796144135087356 60-61 22.78714082649692 26.970074900652758 27.75384570227044 22.488938570579883 62-63 22.761207491697533 27.05507115571707 27.649345066551216 22.53437628603418 64-65 22.573233475155103 27.197921469304493 27.43670301690243 22.792142038637973 66-67 22.538065820345103 27.33098690160335 27.1936802880779 22.93726698997365 68-69 22.534673844350415 27.188580524066307 27.481698832632716 22.79504679895056 70-71 22.61575724518486 27.178875380496798 27.238617504870483 22.966749869447867 72-73 22.633871205481512 27.116407536774712 27.398391337614207 22.851329920129565 74-75 22.560532408150635 27.607419930996585 27.075965829152487 22.75608183170029 76-77 22.745356244738034 27.283069448908527 27.05878323351975 22.91279107283369 78-79 22.69540718986047 27.34316922278085 27.11180248632089 22.849621101037787 80-81 22.7149596880352 27.5552697570213 26.980961475601074 22.748809079342422 82-83 22.697790034977864 27.458907125210967 27.066170535430373 22.777132304380793 84-85 22.648951223419804 27.43815636438795 26.96721281401842 22.94567959817383 86-87 22.793699052142845 27.4135625328289 26.942049306182746 22.850689108845504 88-89 22.694570775592567 27.37237156899464 26.80387375887609 23.129183896536702 90-91 22.63400569319721 27.302427405620428 27.037183120985027 23.026383780197335 92-93 22.705200268955707 27.314384329630354 27.141899207656984 22.83851619375696 94-95 22.476928021174757 27.247150039950352 27.163610533438888 23.112311405436 96-97 22.434713529658545 27.192727641883636 27.180618868404537 23.19193996005328 98-99 22.440790773372836 27.27083125637994 26.853646498149548 23.434731472097674 100-101 22.33921533610105 27.20192851079237 27.09813925496806 23.36071689813852 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 191.0 1 141.0 2 148.5 3 217.0 4 194.5 5 194.0 6 307.5 7 578.0 8 907.5 9 1112.0 10 1122.5 11 1104.0 12 1192.5 13 1331.5 14 1455.5 15 1591.5 16 1764.5 17 1918.0 18 2079.0 19 2298.5 20 2589.5 21 2988.5 22 3479.0 23 4172.0 24 5142.5 25 6341.0 26 7974.5 27 10050.0 28 12177.5 29 14698.5 30 17968.5 31 21594.0 32 25379.5 33 28127.0 34 30973.0 35 33750.0 36 36721.5 37 40224.0 38 42350.0 39 43893.5 40 45504.0 41 48135.5 42 51037.5 43 53916.5 44 56487.5 45 59090.5 46 60633.0 47 63033.0 48 69888.0 49 70094.0 50 63996.5 51 60184.5 52 57143.5 53 56211.5 54 55363.0 55 51674.0 56 46889.5 57 42167.0 58 38311.0 59 34152.5 60 28813.5 61 23825.0 62 19915.0 63 15851.0 64 11895.0 65 9281.5 66 7177.0 67 5763.0 68 4513.5 69 3238.5 70 2428.5 71 1895.5 72 1519.5 73 1281.0 74 1034.0 75 837.0 76 647.5 77 430.0 78 268.5 79 168.0 80 110.0 81 74.0 82 52.5 83 37.0 84 21.5 85 15.0 86 13.0 87 10.0 88 5.0 89 3.5 90 4.0 91 2.0 92 1.5 93 1.0 94 1.0 95 1.0 96 0.5 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0779015737463136 2 0.03369212490911993 3 0.016693194374210053 4 0.022502181427506596 5 0.027516254462983603 6 0.03344753598056007 7 0.08114237704973166 8 0.059129373479344764 9 0.0745384759786156 10-11 0.06484663968443136 12-13 0.04268076803369457 14-15 0.05102736522079959 16-17 0.06414344651482179 18-19 0.05952683048825454 20-21 0.019811703213348197 22-23 0.014950498258221092 24-25 0.02418373031135559 26-27 0.027332812766563717 28-29 0.007215373392515701 30-31 0.0010700765624493624 32-33 0.011281664329823278 34-35 0.012596329820832496 36-37 0.0048917785711970856 38-39 0.011159369865543351 40-41 0.004952925803337049 42-43 0.0023235948213186156 44-45 3.3630977676979965E-4 46-47 3.9745700890976316E-4 48-49 0.0010089293303093987 50-51 0.0010089293303093987 52-53 2.1401531248987248E-4 54-55 2.445889285598543E-4 56-57 0.0040051437051676145 58-59 0.004280306249797449 60-61 0.0059618551336464475 62-63 0.0024458892855985428 64-65 6.726195535395993E-4 66-67 3.668833928397814E-4 68-69 0.00308793522306816 70-71 0.0021401531248987247 72-73 0.022869064820346376 74-75 0.015928853972460508 76-77 0.026446177900534246 78-79 0.02020916022225796 80-81 0.01299378682974226 82-83 0.004708336874777195 84-85 0.003852275624817705 86-87 0.0014675335713591256 88-89 0.0021095795088287433 90-91 0.0068484899996759194 92-93 0.011220517097683314 94-95 0.014828203793941166 96-97 0.01339124383865202 98-99 0.02384742053458579 100-101 0.02158497294540714 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1635397.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.18437859159064 #Duplication Level Percentage of deduplicated Percentage of total 1 82.33559419323937 53.66994543455688 2 10.919874786992107 14.236105045761175 3 3.0259954018716457 5.9174288967604145 4 1.2531222422890742 3.267359786516559 5 0.6604067947087812 2.1524103265378036 6 0.40489771253183066 1.5835803470726333 7 0.266302862687269 1.2151150635001924 8 0.16980815833379548 0.8855071424616714 9 0.1335658978116432 0.7835769044891873 >10 0.7473581874759421 8.87362533865649 >50 0.05573796935755448 2.5245054498201775 >100 0.024790963570101014 3.0425952774279468 >500 0.0017908056847225208 0.7133587919270747 >1k 7.540234461989561E-4 1.1348861945118356 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4827 0.29515768953960414 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4015 0.24550613704195373 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2557 0.15635347258188684 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2226 0.1361137387435589 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 6.114723213996357E-5 0.0 0.0 0.0 0.0 8 6.114723213996357E-5 0.0 0.0 0.0 0.0 9 6.114723213996357E-5 0.0 0.0 0.0 0.0 10-11 6.114723213996357E-5 0.0 0.0 0.0 0.0 12-13 6.114723213996357E-5 0.0 0.0 0.0 0.0 14-15 1.834416964198907E-4 0.0 0.0 6.114723213996357E-5 0.0 16-17 1.834416964198907E-4 0.0 0.0 1.2229446427992714E-4 0.0 18-19 1.834416964198907E-4 0.0 0.0 2.1401531248987248E-4 6.114723213996357E-5 20-21 1.834416964198907E-4 0.0 0.0 2.445889285598543E-4 6.114723213996357E-5 22-23 1.834416964198907E-4 0.0 0.0 2.445889285598543E-4 6.114723213996357E-5 24-25 1.834416964198907E-4 0.0 0.0 3.0573616069981785E-4 1.2229446427992714E-4 26-27 1.834416964198907E-4 0.0 0.0 4.891778571197086E-4 1.2229446427992714E-4 28-29 1.834416964198907E-4 0.0 0.0 6.726195535395993E-4 1.2229446427992714E-4 30-31 1.834416964198907E-4 0.0 0.0 0.0017426961159889617 1.2229446427992714E-4 32-33 1.834416964198907E-4 0.0 0.0 0.0031796560712781053 1.2229446427992714E-4 34-35 1.834416964198907E-4 3.0573616069981786E-5 0.0 0.0040968645533775595 1.834416964198907E-4 36-37 1.834416964198907E-4 6.114723213996357E-5 0.0 0.007001358080025829 1.834416964198907E-4 38-39 1.834416964198907E-4 6.114723213996357E-5 0.0 0.01063961839235366 1.834416964198907E-4 40-41 1.834416964198907E-4 6.114723213996357E-5 0.0 0.015470249731410785 1.834416964198907E-4 42-43 2.445889285598543E-4 6.114723213996357E-5 0.0 0.022288166115016723 1.834416964198907E-4 44-45 3.0573616069981785E-4 6.114723213996357E-5 0.0 0.027699696159403497 1.834416964198907E-4 46-47 3.3630977676979965E-4 6.114723213996357E-5 0.0 0.03950111196241647 1.834416964198907E-4 48-49 3.668833928397814E-4 6.114723213996357E-5 0.0 0.05246432517608875 1.834416964198907E-4 50-51 3.668833928397814E-4 6.114723213996357E-5 0.0 0.0589153581668549 2.445889285598543E-4 52-53 3.668833928397814E-4 6.114723213996357E-5 0.0 0.06952440294313858 2.445889285598543E-4 54-55 3.668833928397814E-4 6.114723213996357E-5 0.0 0.08866348660294718 2.445889285598543E-4 56-57 4.2803062497974497E-4 6.114723213996357E-5 0.0 0.11990972222646856 2.445889285598543E-4 58-59 4.2803062497974497E-4 6.114723213996357E-5 0.0 0.1524400497249292 2.445889285598543E-4 60-61 4.2803062497974497E-4 6.114723213996357E-5 0.0 0.1916354255266458 2.445889285598543E-4 62-63 4.2803062497974497E-4 6.114723213996357E-5 0.0 0.22887408989988364 3.0573616069981785E-4 64-65 4.2803062497974497E-4 6.114723213996357E-5 0.0 0.26858921717479 3.0573616069981785E-4 66-67 4.891778571197086E-4 6.114723213996357E-5 0.0 0.3294918603861937 3.0573616069981785E-4 68-69 4.891778571197086E-4 6.114723213996357E-5 0.0 0.41014505957880565 3.0573616069981785E-4 70-71 4.891778571197086E-4 6.114723213996357E-5 0.0 0.45233664975538046 3.0573616069981785E-4 72-73 4.891778571197086E-4 6.114723213996357E-5 0.0 0.5098150479669463 3.9745700890976316E-4 74-75 4.891778571197086E-4 6.114723213996357E-5 0.0 0.5743865251067478 4.2803062497974497E-4 76-77 4.891778571197086E-4 6.114723213996357E-5 0.0 0.6407924192107483 4.2803062497974497E-4 78-79 4.891778571197086E-4 6.114723213996357E-5 0.0 0.7160035147429034 4.2803062497974497E-4 80-81 4.891778571197086E-4 6.114723213996357E-5 0.0 0.8299208082196555 4.891778571197085E-4 82-83 4.891778571197086E-4 6.114723213996357E-5 0.0 0.9645975870079253 6.114723213996357E-4 84-85 4.891778571197086E-4 6.114723213996357E-5 0.0 1.15815915034698 6.114723213996357E-4 86-87 4.891778571197086E-4 6.114723213996357E-5 0.0 1.4327713698875564 6.114723213996357E-4 88-89 4.891778571197086E-4 6.114723213996357E-5 0.0 1.7341660771054368 6.114723213996357E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTAATA 65 0.005829464 29.246473 2 GTATCAA 10340 0.0 26.980982 1 GTACATG 7630 0.0 25.102715 1 GTACCTG 1455 0.0 24.171795 1 TACATGG 7815 0.0 24.021217 2 TACCTGG 1485 0.0 23.682753 2 ACATGGG 7990 0.0 22.78132 3 TCAACGC 12305 0.0 22.671663 4 TATCAAC 12220 0.0 22.557121 2 ATCAACG 12310 0.0 22.43081 3 CAACGCA 12470 0.0 22.258022 5 GAGTACT 5440 0.0 22.017519 12-13 GTACACG 325 1.8189894E-12 21.935526 1 AACGCAG 12700 0.0 21.92977 6 GGTATCA 7565 0.0 21.234789 1 AGTACTT 5595 0.0 20.812906 12-13 GTACTTT 5820 0.0 20.12678 14-15 CTAGTAC 380 0.0 20.010746 3 ACGCAGA 13850 0.0 19.902992 7 TATCCCG 120 0.0047487216 19.802906 5 >>END_MODULE