FastQCFastQC Report
Thu 26 May 2016
SRR936428_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936428_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1635397
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63870.3905473716779473No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61560.37642236105361576No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35150.21493252097197196No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28580.17475878945601586No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18200.11128796249473369No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG15900.026.5929051
TACCTGG15200.026.5640982
GGTATCA78650.026.0346321
GTATCAA108050.025.3262311
GAGTACT73100.021.89605512-13
ATCACGC2005.392194E-721.3736723
ATCAACG129200.021.0648673
TCAACGC130300.020.9560974
CAACGCA131250.020.9129585
GTACATG71200.020.8184071
TATCAAC130750.020.8176982
AACGCAG133850.020.4712526
TATACCG1404.860989E-420.3521425
GTATAGC6600.020.1551931
GTACTTT79200.020.08783514-15
AGTACTT76550.019.91650612-13
TACATGG74150.019.859592
ACATGGG71700.019.6082653
GTGTAGC9700.019.1014271
TATCACG2001.2846092E-519.0011442
Produced by FastQC (version 0.11.5)