##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936428_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1635397 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.906958983048153 31.0 31.0 34.0 30.0 34.0 2 32.06691341613076 33.0 31.0 34.0 30.0 34.0 3 32.14827408879923 33.0 31.0 34.0 30.0 34.0 4 35.84651433260548 37.0 35.0 37.0 35.0 37.0 5 35.676532364924235 37.0 35.0 37.0 33.0 37.0 6 35.76628671814856 37.0 35.0 37.0 33.0 37.0 7 35.71083840804404 37.0 35.0 37.0 33.0 37.0 8 35.68081939736957 37.0 35.0 37.0 33.0 37.0 9 37.317811516102815 39.0 37.0 39.0 34.0 39.0 10-11 37.21400124862648 39.0 37.0 39.0 33.0 39.0 12-13 37.10549823681956 39.0 37.0 39.0 33.0 39.0 14-15 38.23886340747843 40.0 38.0 41.0 33.0 41.0 16-17 38.13512712815298 40.0 37.0 41.0 33.0 41.0 18-19 38.28547349664944 40.0 38.0 41.0 33.5 41.0 20-21 38.306355276425236 40.0 38.0 41.0 34.0 41.0 22-23 38.22371662660504 40.0 38.0 41.0 33.0 41.0 24-25 38.08803458732039 40.0 37.0 41.0 33.0 41.0 26-27 37.89622672659911 40.0 37.0 41.0 33.0 41.0 28-29 37.8102375141938 40.0 37.0 41.0 33.0 41.0 30-31 37.779753173082746 40.0 37.0 41.0 33.0 41.0 32-33 37.61718958760473 40.0 36.5 41.0 33.0 41.0 34-35 37.43980635894526 40.0 36.0 41.0 32.0 41.0 36-37 37.236089157556236 39.0 36.0 41.0 31.5 41.0 38-39 37.06826415848873 39.0 36.0 41.0 31.0 41.0 40-41 36.86949865995841 39.0 35.5 41.0 31.0 41.0 42-43 36.594087246093764 39.0 35.0 41.0 30.0 41.0 44-45 36.30354892420617 39.0 35.0 40.0 30.0 41.0 46-47 35.91310305693358 39.0 35.0 40.0 29.5 41.0 48-49 35.523996619781 38.0 35.0 40.0 27.5 41.0 50-51 35.22058863994492 38.0 34.5 40.0 27.0 41.0 52-53 34.85040849408431 38.0 34.0 40.0 26.0 41.0 54-55 34.66364375133378 38.0 34.0 40.0 26.0 41.0 56-57 34.3687627530196 38.0 33.5 40.0 25.0 41.0 58-59 34.000071847997766 37.0 33.0 40.0 24.0 41.0 60-61 33.58922176083239 37.0 33.0 39.5 23.0 41.0 62-63 33.252021374626466 36.0 32.0 39.0 22.5 40.5 64-65 32.8330338138079 36.0 32.0 39.0 22.0 40.0 66-67 32.42462136105178 35.0 31.0 38.5 20.5 40.0 68-69 32.06881784667576 35.0 31.0 38.0 20.0 40.0 70-71 31.695820036358143 35.0 31.0 37.5 18.5 39.5 72-73 31.23915141094181 34.5 30.5 37.0 18.0 39.0 74-75 30.739761660318564 34.0 30.0 36.5 14.5 39.0 76-77 29.24781994830613 32.5 28.0 35.0 12.0 37.0 78-79 30.018773423211613 34.0 29.5 35.0 11.0 37.0 80-81 29.79561017905744 34.0 29.0 35.0 7.0 37.0 82-83 29.50994712598837 34.0 29.0 35.0 7.0 36.5 84-85 29.137247408427434 34.0 29.0 35.0 2.0 36.0 86-87 28.756651748780264 34.0 29.0 35.0 2.0 36.0 88-89 28.293542485402625 33.0 28.0 35.0 2.0 35.0 90-91 27.832103152934728 33.0 27.0 35.0 2.0 35.0 92-93 27.218691547067777 33.0 26.0 34.5 2.0 35.0 94-95 26.688843748643297 32.0 25.0 34.0 2.0 35.0 96-97 25.981884215270057 32.0 24.5 34.0 2.0 35.0 98-99 25.101163203797 32.0 20.5 34.0 2.0 35.0 100-101 21.86505631354344 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.248091508960524 1101 2 0.2673765907467214 1101 3 0.23435241516281025 1101 4 0.2510305623007909 1101 5 0.15257632590011383 1101 6 0.1667860305666906 1101 7 0.13845757192181907 1101 8 0.17199200722856745 1101 9 0.2845659261289626 1101 10-11 0.3108543473733292 1101 12-13 0.378749567192898 1101 14-15 0.507193859913528 1101 16-17 0.3819200352790091 1101 18-19 0.27940558992138875 1101 20-21 0.29135974614627713 1101 22-23 0.28728670677314483 1101 24-25 0.35705268430017867 1101 26-27 0.470572488344537 1101 28-29 0.40206778200853677 1101 30-31 0.5500960631887111 1101 32-33 0.47063217687568937 1101 34-35 0.311999827453711 1101 36-37 0.2824064153049477 1101 38-39 0.23471065633287935 1101 40-41 0.34056825324266526 1101 42-43 0.20926806750616578 1101 44-45 0.01345915527314645 1101 46-47 -0.014156002995406425 1101 48-49 -0.05812284798638956 1101 50-51 0.06606859496880446 1101 52-53 -0.1112900159545589 1101 54-55 -0.14620851868900786 1101 56-57 -0.018475803228305665 1101 58-59 -0.3216561749439819 1101 60-61 0.019214283549167988 1101 62-63 0.020970478124233694 1101 64-65 -0.04476321597721977 1101 66-67 -0.2994548707202185 1101 68-69 -0.2688813584456433 1101 70-71 -0.1850603981409229 1101 72-73 -0.05943417098309567 1101 74-75 -0.1483816383927632 1101 76-77 -0.32579521358816166 1101 78-79 -0.14400042706215999 1101 80-81 -0.11501535502364746 1101 82-83 -0.08891762779066426 1101 84-85 0.013802503072056993 1101 86-87 0.11303235701969427 1101 88-89 0.07807229846153874 1101 90-91 0.09668455004369036 1101 92-93 0.02684756043664649 1101 94-95 -0.028792838965408407 1101 96-97 0.171298578868047 1101 98-99 -0.006653180549204052 1101 100-101 0.7132239830274649 1102 1 0.2538517556517128 1102 2 0.2591909116644615 1102 3 0.28253630590782564 1102 4 0.22254446118957105 1102 5 0.2849995942789292 1102 6 0.22954611177458872 1102 7 0.29741591515154653 1102 8 0.21396546221851054 1102 9 0.32254014985554136 1102 10-11 0.3498179003771895 1102 12-13 0.3454751620556422 1102 14-15 0.4747965472628124 1102 16-17 0.47478146491182116 1102 18-19 0.3997128734526356 1102 20-21 0.32711422731269835 1102 22-23 0.2921102497362398 1102 24-25 0.3083470399538868 1102 26-27 0.31792191485023835 1102 28-29 0.3530918689863043 1102 30-31 0.4596872325284096 1102 32-33 0.4638281125067323 1102 34-35 0.40349019853058365 1102 36-37 0.36738953631980564 1102 38-39 0.5235268112277254 1102 40-41 0.6388082623830513 1102 42-43 0.7345849928452566 1102 44-45 0.7143724802174418 1102 46-47 0.8578749613188847 1102 48-49 0.8319431559398751 1102 50-51 0.7932596108095638 1102 52-53 0.8764524224991632 1102 54-55 0.7748750460259828 1102 56-57 0.738661478666728 1102 58-59 0.8092709133666531 1102 60-61 1.0840464763517943 1102 62-63 0.9370858184252526 1102 64-65 0.7979919535127493 1102 66-67 0.759773710137928 1102 68-69 0.7956925189768782 1102 70-71 0.7089202325522201 1102 72-73 0.7931787418674361 1102 74-75 0.632424255917126 1102 76-77 0.6242844077811007 1102 78-79 0.7453851704272125 1102 80-81 0.7317322152749277 1102 82-83 0.8967544066820174 1102 84-85 0.8656134584384496 1102 86-87 0.9631398901109165 1102 88-89 0.831437306476456 1102 90-91 0.7440658934016469 1102 92-93 0.8520616563840449 1102 94-95 0.5648658910232953 1102 96-97 0.7904960687380154 1102 98-99 0.8208202435484537 1102 100-101 1.4399528565085014 1103 1 0.23698126104467931 1103 2 0.29171314811436844 1103 3 0.2621386707512272 1103 4 0.2318842747494898 1103 5 0.1772966968767804 1103 6 0.11993672411325917 1103 7 0.0791671554239386 1103 8 0.15876569777676508 1103 9 0.15085451326945076 1103 10-11 0.3464287822424623 1103 12-13 0.17975654612661884 1103 14-15 0.2706213246905378 1103 16-17 0.18768445326035277 1103 18-19 0.1381381356506992 1103 20-21 0.08128191059607559 1103 22-23 0.11171510195134005 1103 24-25 0.09026153254490765 1103 26-27 0.014214870245552902 1103 28-29 0.12988335540757845 1103 30-31 0.15290169442798884 1103 32-33 0.21331104789027933 1103 34-35 0.20942355952777092 1103 36-37 0.1735786098124379 1103 38-39 0.27557755717209176 1103 40-41 0.23264495307451938 1103 42-43 0.20788032697526404 1103 44-45 0.302687680670509 1103 46-47 0.505380109703367 1103 48-49 0.23981408450125485 1103 50-51 0.36982747123654036 1103 52-53 0.39552845593086516 1103 54-55 0.18773244245425502 1103 56-57 0.040149182205269085 1103 58-59 0.05531352875190976 1103 60-61 0.019364572010537984 1103 62-63 0.1353927137876454 1103 64-65 0.16477034412984182 1103 66-67 0.08305099746615952 1103 68-69 0.20745953832791741 1103 70-71 0.08954390390096378 1103 72-73 0.08005016849680402 1103 74-75 -0.02864196575340472 1103 76-77 0.15644392726125744 1103 78-79 -0.06378800703874532 1103 80-81 0.09038642846108402 1103 82-83 0.17899629437748388 1103 84-85 0.4894479110890444 1103 86-87 0.4785204846936715 1103 88-89 0.19875532021221076 1103 90-91 0.10516636837684956 1103 92-93 0.2532490350207901 1103 94-95 0.3871468939743039 1103 96-97 0.5029033636827158 1103 98-99 0.4110502452878322 1103 100-101 1.0584661740390118 1104 1 0.1831734370920053 1104 2 0.20691789244209957 1104 3 0.22628954102091825 1104 4 0.1916764211794657 1104 5 0.12477421922427823 1104 6 0.05179833014825874 1104 7 0.12104271978756742 1104 8 0.06685909883662333 1104 9 0.11292754199537569 1104 10-11 0.19864982247056417 1104 12-13 0.11279301189862423 1104 14-15 0.2440741801897346 1104 16-17 0.33390734572916614 1104 18-19 0.09866406326059973 1104 20-21 0.08751297675669889 1104 22-23 0.11013748687611269 1104 24-25 0.0708670914651961 1104 26-27 0.12143152282663294 1104 28-29 -0.005426385862968175 1104 30-31 0.16456612533253434 1104 32-33 0.23552940404689338 1104 34-35 0.09101956396342814 1104 36-37 0.06342712174353693 1104 38-39 0.11036008664336805 1104 40-41 0.09721836214031754 1104 42-43 0.029486032246857974 1104 44-45 0.07788122015672627 1104 46-47 0.18551789396764917 1104 48-49 0.27740916223809364 1104 50-51 0.3466928286085178 1104 52-53 0.4851826619113666 1104 54-55 0.2747602787050809 1104 56-57 0.16107600250031595 1104 58-59 0.15911739022836713 1104 60-61 0.3580141311922844 1104 62-63 0.23994235956541132 1104 64-65 0.2694922274687528 1104 66-67 0.19327580580668524 1104 68-69 0.17206204411689185 1104 70-71 0.07284886359072118 1104 72-73 0.21637959662182382 1104 74-75 0.144787230581219 1104 76-77 0.18257775946166888 1104 78-79 0.017675008596548025 1104 80-81 0.4300138804963254 1104 82-83 0.23273238883366076 1104 84-85 0.6127527955785261 1104 86-87 0.7351939309546829 1104 88-89 0.5849428801193568 1104 90-91 0.5130327224417428 1104 92-93 0.6175173044946227 1104 94-95 0.6512269892125673 1104 96-97 0.6898937733333383 1104 98-99 0.6045699191268739 1104 100-101 1.3492177834695873 1105 1 -0.014889235451224891 1105 2 0.0982754176772147 1105 3 0.06190816101698715 1105 4 0.15649597906994472 1105 5 0.19886474369870655 1105 6 0.17195992605024202 1105 7 0.11236520218779589 1105 8 0.1370507518042956 1105 9 0.07547957205814271 1105 10-11 0.149099966203174 1105 12-13 0.14031673703744474 1105 14-15 0.17390304010101687 1105 16-17 0.15863006573691507 1105 18-19 0.12681499816354602 1105 20-21 0.13821055709168917 1105 22-23 0.16969962238076874 1105 24-25 0.16452747226399822 1105 26-27 0.08357130100263532 1105 28-29 0.058289180278244146 1105 30-31 0.13734838397144244 1105 32-33 0.11756938136876727 1105 34-35 0.178035623838241 1105 36-37 0.14774787479412055 1105 38-39 0.08617084393897301 1105 40-41 0.20191951513506012 1105 42-43 0.23901568902650894 1105 44-45 0.3542013806130271 1105 46-47 0.16938807593398764 1105 48-49 0.1716070089556112 1105 50-51 0.39756063734871105 1105 52-53 0.4100871488187394 1105 54-55 0.33023565042977765 1105 56-57 0.34629020538978494 1105 58-59 0.2865284369834953 1105 60-61 0.3234373945064206 1105 62-63 0.22635086785175673 1105 64-65 0.3220700125906504 1105 66-67 0.3041812499817311 1105 68-69 0.10005158254862323 1105 70-71 0.2680095335641397 1105 72-73 0.4357786547387974 1105 74-75 0.3525993611001077 1105 76-77 0.13791053818753696 1105 78-79 0.15980817706711647 1105 80-81 0.23085946695098158 1105 82-83 0.40043486092047686 1105 84-85 0.365787277158244 1105 86-87 0.41425355790518736 1105 88-89 0.20367469986813092 1105 90-91 0.20465947921313088 1105 92-93 0.22548712777047442 1105 94-95 0.5418221191132204 1105 96-97 0.4948738445016332 1105 98-99 0.47521556199195203 1105 100-101 1.108890033248077 1106 1 0.07577092061177382 1106 2 0.09381711848794083 1106 3 0.02871562793596638 1106 4 0.14761739675952867 1106 5 0.14876547952082575 1106 6 -0.019776788741950213 1106 7 -0.02173971652821649 1106 8 -0.051374904254480214 1106 9 -0.00362933130501375 1106 10-11 0.06572129518859526 1106 12-13 0.07692254983506785 1106 14-15 0.19572848721222158 1106 16-17 0.07421482326480344 1106 18-19 0.19355389535964918 1106 20-21 0.14211325848533107 1106 22-23 0.07670739173869379 1106 24-25 0.09082586179071228 1106 26-27 0.016042936791805573 1106 28-29 -0.14589568482103488 1106 30-31 -0.10807828303314437 1106 32-33 -0.03150021056394081 1106 34-35 0.013246246935679551 1106 36-37 -0.2277002220088704 1106 38-39 -0.09947625994063714 1106 40-41 0.014888907976207122 1106 42-43 0.0626200065254423 1106 44-45 0.002818299734549612 1106 46-47 -0.010180540252974879 1106 48-49 -0.08949818854490132 1106 50-51 0.05577267126467689 1106 52-53 -0.15203772410904293 1106 54-55 -4.790798110221317E-4 1106 56-57 -0.02986752468486742 1106 58-59 -0.024376964958989333 1106 60-61 -0.12592576595235272 1106 62-63 0.1658171785753879 1106 64-65 0.23206274729762555 1106 66-67 0.20338263095060682 1106 68-69 0.10714143845364532 1106 70-71 0.3139599775073343 1106 72-73 0.20700795358170154 1106 74-75 0.1960253973195698 1106 76-77 0.07997559916812591 1106 78-79 0.018249856194607617 1106 80-81 0.2178129155715638 1106 82-83 0.18057864613813734 1106 84-85 0.49255129311935875 1106 86-87 0.6085473460796571 1106 88-89 0.40224318370285417 1106 90-91 0.5978875502778322 1106 92-93 0.5671992025185126 1106 94-95 0.5793031462792229 1106 96-97 0.57845315587576 1106 98-99 0.4649955756777331 1106 100-101 0.7618472609474303 1107 1 0.091154441542173 1107 2 0.1758543619009174 1107 3 0.1560706203125335 1107 4 0.12829713223817407 1107 5 0.025265126096456925 1107 6 -0.11526261151735184 1107 7 -0.03785736525461658 1107 8 0.10032264258193635 1107 9 0.026556559480837905 1107 10-11 0.1184827523293066 1107 12-13 0.07479825945850394 1107 14-15 0.1526022902429247 1107 16-17 0.22625322470084797 1107 18-19 0.07467894821284204 1107 20-21 0.06498437479550745 1107 22-23 -0.027899935471822346 1107 24-25 0.0835398105665135 1107 26-27 0.022575871850783358 1107 28-29 0.10923471847320343 1107 30-31 0.07656207859098885 1107 32-33 0.15313386617741997 1107 34-35 0.11215117965935661 1107 36-37 0.16391015484072824 1107 38-39 0.20373724073638755 1107 40-41 0.20628123950992716 1107 42-43 0.06286177393075576 1107 44-45 0.22276111532458742 1107 46-47 -0.014839510478942941 1107 48-49 0.0784468500215425 1107 50-51 0.22318576805107426 1107 52-53 0.10412861507511906 1107 54-55 -0.08713357252603515 1107 56-57 -0.07031422205920279 1107 58-59 0.09840517345766386 1107 60-61 0.10640575706730004 1107 62-63 -0.004396301508627687 1107 64-65 0.0050010316209139205 1107 66-67 0.15684160310215844 1107 68-69 0.08765950535207878 1107 70-71 0.14896134547224804 1107 72-73 0.17445012129392978 1107 74-75 0.12889816909461516 1107 76-77 0.1800056596286943 1107 78-79 0.31234305592470335 1107 80-81 0.489799508178244 1107 82-83 0.3098902428701038 1107 84-85 0.6343335681987838 1107 86-87 0.6494057701359388 1107 88-89 0.45861898332046636 1107 90-91 0.4712444880262012 1107 92-93 0.6695285940268612 1107 94-95 0.662800220866874 1107 96-97 0.7207454425059936 1107 98-99 0.7307125296972643 1107 100-101 1.4249985386106054 1108 1 0.10557762163535145 1108 2 0.18478476582698988 1108 3 0.10361591473799336 1108 4 0.054049875049692275 1108 5 0.2193437675745784 1108 6 0.14565127198682148 1108 7 0.09101364570782522 1108 8 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2316 72-73 -1.0742572400674426 2316 74-75 -1.2604161688976951 2316 76-77 -1.339638725602665 2316 78-79 -1.2383403102231938 2316 80-81 -0.8475908297305779 2316 82-83 -1.195329185669518 2316 84-85 -0.9268011778999927 2316 86-87 -1.1668210886408552 2316 88-89 -1.6663403173467266 2316 90-91 -1.6348998937265833 2316 92-93 -1.7991374418581252 2316 94-95 -1.533882957437946 2316 96-97 -1.663050483386435 2316 98-99 -2.31888743412555 2316 100-101 -3.275946242482636 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 7.0 4 8.0 5 53.0 6 121.0 7 294.0 8 671.0 9 1259.0 10 2108.0 11 3263.0 12 4768.0 13 6512.0 14 8286.0 15 10965.0 16 14037.0 17 17952.0 18 15735.0 19 10886.0 20 9880.0 21 10001.0 22 10674.0 23 12010.0 24 14281.0 25 16850.0 26 21063.0 27 25960.0 28 32447.0 29 39911.0 30 49202.0 31 61093.0 32 76991.0 33 96854.0 34 127587.0 35 172725.0 36 244132.0 37 315321.0 38 190411.0 39 11076.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.27665177815113 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22.072433451124727 26.621170531320914 29.618002454479914 21.68839356307445 20-21 22.835280974589047 25.576266802495052 29.835416109972073 21.75303611294383 22-23 22.414686591294526 25.600825243297265 29.77733534344729 22.207152821960914 24-25 22.465584809070823 25.736044520076778 29.899620703719037 21.898749967133362 26-27 22.286634824875243 25.87843659290793 29.820004806231225 22.014923775985597 28-29 21.91196263065238 26.18954585284749 29.951368238378183 21.947123278121943 30-31 22.13670698143124 25.81716431828424 29.809567311837426 22.236561388447097 32-33 21.991396498853856 25.918803961071742 29.795883173639492 22.29391636643491 34-35 22.65036091143705 25.890538155351056 29.471646691107566 21.987454242104327 36-37 22.524899289954433 25.685861703766932 29.556869684747593 22.23236932153104 38-39 22.31250724221609 25.91415493618748 29.69034959228043 22.082988229316 40-41 22.477166038988795 25.9501233036952 29.485084013445086 22.087626643870923 42-43 22.303786678016184 26.07312855283885 29.147404287377576 22.475680481767395 44-45 22.597892894672555 26.08670262383807 28.912360280217676 22.403044201271697 46-47 22.8294474100979 26.48597582228092 28.364885440170234 22.319691327450943 48-49 22.97666455197927 26.515581169600477 28.057539879692122 22.45021439872813 50-51 22.756144010600508 26.693728451287107 28.102288531781493 22.44783900633089 52-53 23.104063123703675 26.857689165655696 27.668993122138303 22.36925458850233 54-55 22.656178102978014 26.677167867670192 27.928812374703316 22.737841654648477 56-57 22.835237724808703 26.998693712205718 27.63131983991859 22.53474872306699 58-59 23.146392542022078 26.61002560285362 27.658360316433168 22.585221538691137 60-61 22.87283897197219 26.553440290873954 28.25143839187559 22.322282345278268 62-63 22.77753417519938 26.708240147702668 28.147564453267137 22.366661223830814 64-65 22.57905899793775 26.791196667131466 27.99264635730484 22.637097977625942 66-67 22.53955713618319 26.946151174423182 27.704854478168667 22.809437211224957 68-69 22.632428418166135 26.7215304544775 27.987410903925593 22.658630223430773 70-71 22.72012821379075 26.816023177296472 27.73473173276034 22.729116876152435 72-73 22.75808259401234 26.68572218244255 27.798265497613116 22.75792972593199 74-75 22.634334320164342 27.095986564504233 27.51980515718313 22.749873958148296 76-77 22.81915822230022 26.83182087374619 27.57538781204511 22.773633091908483 78-79 22.769095481074523 26.85481661586932 27.589839144452 22.78624875860416 80-81 22.86085770873423 27.075126189847875 27.350057533458237 22.713958567959658 82-83 22.767009811191517 27.01663025247344 27.494514936954268 22.72184499938078 84-85 22.6677496905828 27.035616542252463 27.411963275458223 22.88467049170652 86-87 22.725509003787543 27.013808773513915 27.295971174670697 22.964711048027844 88-89 22.616409202993278 26.97860012271147 27.155081973831745 23.2499087004635 90-91 22.60384820673444 26.81115157150657 27.438041302229976 23.146958919529016 92-93 22.69910788584144 26.699477319149707 27.51571697127075 23.08569782373811 94-95 22.440858311030862 26.662042554675082 27.648244534785892 23.248854599508167 96-97 22.398847422683566 26.59477333104939 27.721289185869917 23.28509006039713 98-99 22.362707214769205 26.619848079210247 27.353664893278584 23.663779812741964 100-101 22.049075989744402 26.64304775167039 27.506654482969388 23.801221775615822 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 203.0 1 169.0 2 221.5 3 299.5 4 235.0 5 207.5 6 361.5 7 732.5 8 1173.0 9 1433.0 10 1412.5 11 1337.5 12 1432.5 13 1544.5 14 1718.5 15 1980.0 16 2223.5 17 2422.0 18 2618.0 19 2877.5 20 3166.0 21 3525.0 22 3979.0 23 4779.5 24 5702.0 25 6791.5 26 8349.5 27 10398.0 28 12614.0 29 15032.5 30 18111.5 31 21583.0 32 25275.5 33 27960.5 34 30640.0 35 33479.5 36 36382.5 37 39527.0 38 41793.5 39 43432.0 40 45027.5 41 47736.5 42 50550.5 43 53249.5 44 55727.5 45 58153.5 46 59981.0 47 62954.0 48 70224.0 49 70352.0 50 63670.5 51 60146.5 52 57479.5 53 56103.0 54 54741.5 55 51088.5 56 46578.5 57 42253.5 58 38569.0 59 34246.5 60 28867.5 61 23809.0 62 19650.0 63 15576.5 64 11603.5 65 8883.0 66 7008.0 67 5716.5 68 4391.5 69 3144.0 70 2329.0 71 1758.5 72 1493.5 73 1316.0 74 1058.5 75 872.5 76 698.5 77 460.0 78 287.5 79 199.0 80 132.5 81 93.5 82 75.0 83 49.0 84 25.5 85 14.5 86 10.0 87 7.0 88 3.0 89 2.0 90 3.0 91 1.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 1.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02176841464182703 2 0.014369599552891438 3 0.010150440535233953 4 0.0 5 0.0029350671427182513 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0011923710267292896 14-15 1.834416964198907E-4 16-17 3.0573616069981786E-5 18-19 0.0012229446427992714 20-21 0.0 22-23 3.0573616069981786E-5 24-25 0.0 26-27 0.0012229446427992714 28-29 0.0028127726784383245 30-31 9.783557142394172E-4 32-33 0.008102008258545172 34-35 0.0014369599552891438 36-37 5.503250892596721E-4 38-39 0.0012535182588692532 40-41 2.445889285598543E-4 42-43 0.005197514731896903 44-45 0.001559254419569071 46-47 4.2803062497974497E-4 48-49 5.808987053296539E-4 50-51 2.1401531248987248E-4 52-53 3.0573616069981785E-4 54-55 0.0017426961159889617 56-57 0.014002716160051658 58-59 0.025865279195204587 60-61 0.01984227682941818 62-63 0.013452391070791986 64-65 0.01767155008844947 66-67 0.0025070365177385064 68-69 8.254876338895082E-4 70-71 2.1401531248987248E-4 72-73 0.0 74-75 0.0016815488838489983 76-77 0.0022318739731086706 78-79 0.008621759731734863 80-81 0.015990001204600472 82-83 0.01678491522242 84-85 0.028647478257572932 86-87 0.01030330861558386 88-89 0.04084635106949566 90-91 0.04521837816750306 92-93 0.028158300400453223 94-95 0.0298704229003722 96-97 0.04888721209590088 98-99 0.052647766872508626 100-101 0.05946568325611457 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1635397.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.2166794872626 #Duplication Level Percentage of deduplicated Percentage of total 1 80.58920909626075 50.139929924740436 2 11.89177651716824 14.797336962056248 3 3.393246131879861 6.333495210256888 4 1.3831882854421704 3.4422952890356724 5 0.7278205671561763 2.264128947549676 6 0.43194184956479537 1.6124392566904961 7 0.2899307319664282 1.2626969196983893 8 0.2082072245884756 1.036316972732295 9 0.16061711233257944 0.8993757058349174 >10 0.8273725489301186 9.438821429373538 >50 0.06309282771561717 2.7299062170240864 >100 0.030740093434842252 3.652467251013948 >500 0.002068871888251415 0.8943472794533469 >1k 5.911062537861186E-4 0.7276263830025825 >5k 1.970354179287062E-4 0.7688162515374773 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6387 0.3905473716779473 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6156 0.37642236105361576 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3515 0.21493252097197196 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2858 0.17475878945601586 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1820 0.11128796249473369 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 6.114723213996357E-5 10-11 0.0 0.0 0.0 0.0 6.114723213996357E-5 12-13 0.0 0.0 0.0 0.0 6.114723213996357E-5 14-15 1.2229446427992714E-4 6.114723213996357E-5 0.0 3.0573616069981786E-5 6.114723213996357E-5 16-17 1.2229446427992714E-4 6.114723213996357E-5 0.0 6.114723213996357E-5 6.114723213996357E-5 18-19 1.2229446427992714E-4 6.114723213996357E-5 0.0 1.5286808034990892E-4 1.2229446427992714E-4 20-21 1.2229446427992714E-4 6.114723213996357E-5 0.0 1.834416964198907E-4 1.834416964198907E-4 22-23 1.2229446427992714E-4 6.114723213996357E-5 0.0 1.834416964198907E-4 1.834416964198907E-4 24-25 1.834416964198907E-4 6.114723213996357E-5 0.0 3.0573616069981785E-4 1.834416964198907E-4 26-27 1.834416964198907E-4 6.114723213996357E-5 0.0 4.891778571197086E-4 1.834416964198907E-4 28-29 3.0573616069981785E-4 6.114723213996357E-5 0.0 7.03193169609581E-4 1.834416964198907E-4 30-31 3.0573616069981785E-4 6.114723213996357E-5 0.0 0.0017732697320589435 1.834416964198907E-4 32-33 3.3630977676979965E-4 6.114723213996357E-5 0.0 0.0032102296873480875 1.834416964198907E-4 34-35 3.668833928397814E-4 1.2229446427992714E-4 0.0 0.004158011785517522 1.834416964198907E-4 36-37 4.2803062497974497E-4 1.2229446427992714E-4 0.0 0.007154226160375738 1.834416964198907E-4 38-39 4.891778571197086E-4 1.2229446427992714E-4 0.0 0.010823060088773552 2.7516254462983604E-4 40-41 4.891778571197086E-4 1.5286808034990892E-4 0.0 0.015745412276040618 3.0573616069981785E-4 42-43 4.891778571197086E-4 1.834416964198907E-4 0.0 0.02259390227571654 3.668833928397814E-4 44-45 4.891778571197086E-4 1.834416964198907E-4 0.0 0.02809715316831326 4.5860424104972677E-4 46-47 5.197514731896903E-4 1.834416964198907E-4 0.0 0.039929142587396216 5.197514731896903E-4 48-49 5.503250892596721E-4 1.834416964198907E-4 0.0 0.05301465026534842 5.503250892596721E-4 50-51 5.503250892596721E-4 1.834416964198907E-4 0.0 0.05952683048825454 5.503250892596721E-4 52-53 5.503250892596721E-4 1.834416964198907E-4 0.0 0.07031931696095811 5.503250892596721E-4 54-55 5.503250892596721E-4 1.834416964198907E-4 0.0 0.08927495892434681 5.503250892596721E-4 56-57 5.503250892596721E-4 1.834416964198907E-4 0.0 0.12079635709249803 5.503250892596721E-4 58-59 5.503250892596721E-4 1.834416964198907E-4 0.0 0.1537241415998684 5.503250892596721E-4 60-61 5.503250892596721E-4 1.834416964198907E-4 0.0 0.19307238548193495 5.503250892596721E-4 62-63 5.503250892596721E-4 1.834416964198907E-4 0.0 0.23003588731054292 5.503250892596721E-4 64-65 5.503250892596721E-4 1.834416964198907E-4 0.0 0.2698427354336592 5.503250892596721E-4 66-67 5.503250892596721E-4 1.834416964198907E-4 0.0 0.33080652587720294 5.503250892596721E-4 68-69 5.808987053296539E-4 1.834416964198907E-4 0.0 0.41167374038230475 5.503250892596721E-4 70-71 6.114723213996357E-4 1.834416964198907E-4 0.0 0.45359016801424973 5.503250892596721E-4 72-73 6.114723213996357E-4 1.834416964198907E-4 0.0 0.5105182411365559 6.114723213996357E-4 74-75 6.114723213996357E-4 1.834416964198907E-4 0.0 0.5747534084995876 6.114723213996357E-4 76-77 6.114723213996357E-4 1.834416964198907E-4 0.0 0.6409147136750282 6.420459374696175E-4 78-79 6.114723213996357E-4 1.834416964198907E-4 0.0 0.7156672049661337 7.03193169609581E-4 80-81 6.114723213996357E-4 1.834416964198907E-4 0.0 0.8286061427286464 7.337667856795628E-4 82-83 6.114723213996357E-4 1.834416964198907E-4 0.0 0.9619682560259069 7.643404017495446E-4 84-85 6.114723213996357E-4 1.834416964198907E-4 0.0 1.1535731079364826 7.949140178195264E-4 86-87 6.114723213996357E-4 1.834416964198907E-4 0.0 1.4258311590396704 7.949140178195264E-4 88-89 6.114723213996357E-4 1.834416964198907E-4 0.0 1.7232207225523832 8.560612499594899E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCTG 1590 0.0 26.592905 1 TACCTGG 1520 0.0 26.564098 2 GGTATCA 7865 0.0 26.034632 1 GTATCAA 10805 0.0 25.326231 1 GAGTACT 7310 0.0 21.896055 12-13 ATCACGC 200 5.392194E-7 21.373672 3 ATCAACG 12920 0.0 21.064867 3 TCAACGC 13030 0.0 20.956097 4 CAACGCA 13125 0.0 20.912958 5 GTACATG 7120 0.0 20.818407 1 TATCAAC 13075 0.0 20.817698 2 AACGCAG 13385 0.0 20.471252 6 TATACCG 140 4.860989E-4 20.352142 5 GTATAGC 660 0.0 20.155193 1 GTACTTT 7920 0.0 20.087835 14-15 AGTACTT 7655 0.0 19.916506 12-13 TACATGG 7415 0.0 19.85959 2 ACATGGG 7170 0.0 19.608265 3 GTGTAGC 970 0.0 19.101427 1 TATCACG 200 1.2846092E-5 19.001144 2 >>END_MODULE