##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936427_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2199199 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.882757313003506 31.0 31.0 34.0 30.0 34.0 2 31.9774317831174 31.0 31.0 34.0 30.0 34.0 3 32.107302704302796 33.0 31.0 34.0 30.0 34.0 4 35.82421463451011 37.0 35.0 37.0 35.0 37.0 5 35.689631543120925 37.0 35.0 37.0 33.0 37.0 6 35.767640854693006 37.0 35.0 37.0 33.0 37.0 7 35.69619438713823 37.0 35.0 37.0 33.0 37.0 8 35.67220247008115 37.0 35.0 37.0 33.0 37.0 9 37.26078904182841 39.0 37.0 39.0 34.0 39.0 10-11 37.1462423364143 39.0 37.0 39.0 33.0 39.0 12-13 37.03471718566624 39.0 37.0 39.0 33.0 39.0 14-15 38.05553931226778 40.0 37.0 41.0 33.0 41.0 16-17 38.06882346708961 40.0 37.0 41.0 33.0 41.0 18-19 38.25866554140848 40.0 38.0 41.0 33.5 41.0 20-21 38.280359803728544 40.0 38.0 41.0 33.5 41.0 22-23 38.18603364224884 40.0 37.5 41.0 33.0 41.0 24-25 38.043299173926506 40.0 37.0 41.0 33.0 41.0 26-27 37.81657435275298 40.0 37.0 41.0 33.0 41.0 28-29 37.853621932349 40.0 37.0 41.0 33.0 41.0 30-31 37.758382256448826 40.0 37.0 41.0 33.0 41.0 32-33 37.56447642982741 40.0 36.5 41.0 32.5 41.0 34-35 37.33743217416887 39.0 36.0 41.0 32.0 41.0 36-37 37.14228112144467 39.0 36.0 41.0 31.0 41.0 38-39 36.929984053284855 39.0 35.5 41.0 31.0 41.0 40-41 36.76599866587789 39.0 35.0 40.5 31.0 41.0 42-43 36.48592351124205 39.0 35.0 40.0 30.0 41.0 44-45 36.186361034176535 39.0 35.0 40.0 30.0 41.0 46-47 35.79615623688443 38.0 35.0 40.0 28.5 41.0 48-49 35.351128297166376 38.0 35.0 40.0 27.0 41.0 50-51 35.01999568933962 38.0 34.0 40.0 26.5 41.0 52-53 34.7793246541127 38.0 34.0 40.0 26.0 41.0 54-55 34.47558361021444 38.0 34.0 40.0 25.0 41.0 56-57 34.08520374918322 37.5 33.0 40.0 23.5 41.0 58-59 33.70462654812047 37.0 33.0 40.0 23.0 41.0 60-61 33.23353070822604 36.5 32.0 39.0 22.0 40.0 62-63 32.93836915167749 36.0 32.0 39.0 22.0 40.0 64-65 32.63651970558371 36.0 31.5 39.0 21.5 40.0 66-67 32.13725792890958 35.0 31.0 38.5 19.5 40.0 68-69 31.65214789566565 35.0 30.5 38.0 18.0 40.0 70-71 31.08808684434651 34.5 30.0 37.0 16.0 39.0 72-73 30.477222388696973 34.0 29.0 36.5 11.5 39.0 74-75 29.9530474504581 34.0 29.0 36.0 8.0 38.5 76-77 28.609102905194117 32.0 27.0 34.5 7.5 36.5 78-79 29.388768819920344 34.0 29.0 35.0 7.0 37.0 80-81 29.17325217044933 34.0 29.0 35.0 4.0 37.0 82-83 28.734741603647507 33.5 28.5 35.0 2.0 36.0 84-85 28.259745480058875 33.0 27.0 35.0 2.0 36.0 86-87 27.9067287680651 33.0 27.0 35.0 2.0 35.0 88-89 27.376453881617806 32.5 26.0 34.5 2.0 35.0 90-91 26.73421163796455 32.0 25.0 34.0 2.0 35.0 92-93 26.14337879382448 32.0 24.5 34.0 2.0 35.0 94-95 25.346743518890285 31.0 21.5 34.0 2.0 35.0 96-97 24.430453997114405 31.0 18.0 34.0 2.0 35.0 98-99 23.294811201714808 31.0 2.0 34.0 2.0 35.0 100-101 19.430535390385316 25.0 2.0 32.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2698173159393136 1101 2 0.36493392536790736 1101 3 0.2709073530813697 1101 4 0.19700606177791258 1101 5 0.17990737759996023 1101 6 0.19951223873565738 1101 7 0.25176445521152147 1101 8 0.1460612579395999 1101 9 0.3561378752263451 1101 10-11 0.3318379244938541 1101 12-13 0.3112850786347465 1101 14-15 0.39883866196507967 1101 16-17 0.33034235961275726 1101 18-19 0.3063315387848107 1101 20-21 0.24587451220796197 1101 22-23 0.2835438887207786 1101 24-25 0.26968678614844777 1101 26-27 0.2886697209534361 1101 28-29 0.2038425953198768 1101 30-31 0.373497545054164 1101 32-33 0.27920333811360365 1101 34-35 0.34854576053160713 1101 36-37 0.30838076160056005 1101 38-39 0.2928369787292837 1101 40-41 0.39903414297987894 1101 42-43 0.3121293629315005 1101 44-45 0.30748192452104917 1101 46-47 0.4400971082342906 1101 48-49 0.45940714862963006 1101 50-51 0.3897720576176269 1101 52-53 0.38221226165165234 1101 54-55 0.35430945718936613 1101 56-57 0.3205751367484879 1101 58-59 0.2862414833979656 1101 60-61 0.20176036632931726 1101 62-63 0.26317621906266453 1101 64-65 0.3206884172402624 1101 66-67 0.20734226103677855 1101 68-69 0.04711526569092328 1101 70-71 0.023858087530928884 1101 72-73 0.3738474532711926 1101 74-75 0.3251798353083508 1101 76-77 0.33360653285979325 1101 78-79 0.4469475093210171 1101 80-81 0.459684805287349 1101 82-83 0.3094051661872861 1101 84-85 0.31959154234411 1101 86-87 0.21333582377597438 1101 88-89 0.29739369215160494 1101 90-91 0.16736169585140814 1101 92-93 0.26900768903363925 1101 94-95 0.44735657697326303 1101 96-97 0.41176542106130753 1101 98-99 -0.3225646884643396 1101 100-101 0.06193738888130795 1102 1 0.2799623796800361 1102 2 0.3905481799509545 1102 3 0.2770523463362622 1102 4 0.17657104677030588 1102 5 0.13735058266867384 1102 6 0.1728734922868682 1102 7 0.28871811665636216 1102 8 0.18571589698365898 1102 9 0.24842036282769442 1102 10-11 0.3233211322253595 1102 12-13 0.2986284679455693 1102 14-15 0.3833087749969266 1102 16-17 0.35080331731930414 1102 18-19 0.25353716746941046 1102 20-21 0.27869765544493674 1102 22-23 0.23405525358085555 1102 24-25 0.27097290768558224 1102 26-27 0.31055410070755585 1102 28-29 0.33746168992947645 1102 30-31 0.40075671359556253 1102 32-33 0.2391248051585464 1102 34-35 0.361494838439981 1102 36-37 0.4037970293370492 1102 38-39 0.42904985374813265 1102 40-41 0.3909865219743196 1102 42-43 0.38257333551251804 1102 44-45 0.6010482820290619 1102 46-47 0.37701058297002277 1102 48-49 0.3206046538230609 1102 50-51 0.37102258381387543 1102 52-53 0.4288298231945902 1102 54-55 0.43060081621478474 1102 56-57 0.5867403750982518 1102 58-59 0.5270298673342424 1102 60-61 0.2969433945177755 1102 62-63 0.2053176371199612 1102 64-65 0.3280715625135002 1102 66-67 0.40783502547120065 1102 68-69 0.37986244087364085 1102 70-71 0.11478039453193034 1102 72-73 0.10228515457185594 1102 74-75 0.3292653209067282 1102 76-77 0.25695099707942504 1102 78-79 0.2576375165849747 1102 80-81 0.44997929897397526 1102 82-83 0.5371272405850718 1102 84-85 0.38768109262497674 1102 86-87 0.48606994929164316 1102 88-89 0.26321541000860194 1102 90-91 0.4146673262981544 1102 92-93 0.4985868690877169 1102 94-95 0.5741989980829842 1102 96-97 0.5967934000173756 1102 98-99 0.5183160123213817 1102 100-101 0.5567902105658824 1103 1 0.2602583954294957 1103 2 0.3788153659646767 1103 3 0.34524063887953105 1103 4 0.1610875517844832 1103 5 0.20251570589523027 1103 6 0.23309315664644004 1103 7 0.22490792237309165 1103 8 0.2019664647811794 1103 9 0.1400876928185113 1103 10-11 0.3218243234103184 1103 12-13 0.23044232028278344 1103 14-15 0.3631318843205307 1103 16-17 0.4145400295861279 1103 18-19 0.30326367932019593 1103 20-21 0.3802901577054101 1103 22-23 0.3785769825705998 1103 24-25 0.34699311214195205 1103 26-27 0.23326396812693417 1103 28-29 0.3635090390399185 1103 30-31 0.5814408891599072 1103 32-33 0.6481634427177525 1103 34-35 0.5151239682683268 1103 36-37 0.6481075749835199 1103 38-39 0.5250543068377667 1103 40-41 0.575234456176382 1103 42-43 0.7504045292878487 1103 44-45 0.8074753131156882 1103 46-47 0.6745511835715021 1103 48-49 0.6918485113222843 1103 50-51 0.7313103123806854 1103 52-53 0.8684518348795791 1103 54-55 0.8768303308187981 1103 56-57 0.9103958488252957 1103 58-59 0.9538652137570622 1103 60-61 0.6919645231793012 1103 62-63 0.8761795311256861 1103 64-65 1.0297793004637583 1103 66-67 0.799315194780128 1103 68-69 0.8527947641748206 1103 70-71 0.7397362060220409 1103 72-73 0.8161457853224157 1103 74-75 0.8976755688596789 1103 76-77 0.721742307358312 1103 78-79 0.6616580871009354 1103 80-81 0.7767537466791659 1103 82-83 0.8174081468431496 1103 84-85 0.7840615988707889 1103 86-87 0.6972752974478489 1103 88-89 0.6716412472706423 1103 90-91 0.6135792522625998 1103 92-93 0.762045795500363 1103 94-95 0.7937603239495914 1103 96-97 0.9704828184640135 1103 98-99 0.7618649665173827 1103 100-101 0.8166806119397485 1104 1 0.24076530954939557 1104 2 0.2668639935094461 1104 3 0.163879254082282 1104 4 0.16902479309074891 1104 5 0.11356731130556597 1104 6 0.1174673428560169 1104 7 0.12654447189676432 1104 8 0.10056403982779472 1104 9 0.14581809509802213 1104 10-11 0.14347308812895676 1104 12-13 0.19661820492495963 1104 14-15 0.1935072001391589 1104 16-17 0.2082838435435761 1104 18-19 0.22907350013270644 1104 20-21 0.21881148990616595 1104 22-23 0.08882262559536258 1104 24-25 0.16670541596955246 1104 26-27 0.2756366610388099 1104 28-29 0.27911647074081714 1104 30-31 0.38575596937483425 1104 32-33 0.25290733952636657 1104 34-35 0.2559992620976246 1104 36-37 0.09891604925855546 1104 38-39 0.34168291631999637 1104 40-41 0.4471650713652835 1104 42-43 0.4359419097445567 1104 44-45 0.47173740152201304 1104 46-47 0.41490153367949034 1104 48-49 0.4941906464810515 1104 50-51 0.4213078202568994 1104 52-53 0.47203243861262223 1104 54-55 0.35911179190502196 1104 56-57 0.43774834861653034 1104 58-59 0.479437072161609 1104 60-61 0.511977183102239 1104 62-63 0.40787043464327155 1104 64-65 0.5316026851499984 1104 66-67 0.4638050003796437 1104 68-69 0.617576657223669 1104 70-71 0.5059625590533621 1104 72-73 0.6177249649376684 1104 74-75 0.7416397125845577 1104 76-77 0.5370530396160973 1104 78-79 0.5949698923061355 1104 80-81 0.6116341568567201 1104 82-83 0.4433072591496483 1104 84-85 0.4837267516504973 1104 86-87 0.745906548284804 1104 88-89 0.7324841517264389 1104 90-91 0.6757943895838885 1104 92-93 0.7028523376794347 1104 94-95 0.5829441937648667 1104 96-97 0.7742565659082672 1104 98-99 0.707781844882799 1104 100-101 0.4462700604885903 1105 1 0.1404367612716868 1105 2 0.3093018714693585 1105 3 0.144104519364177 1105 4 0.1643706392201949 1105 5 0.17464196933103437 1105 6 0.11087251587822067 1105 7 0.1252635167407803 1105 8 0.10490446638938522 1105 9 0.132645917114651 1105 10-11 0.2204460917847939 1105 12-13 0.14495343372912828 1105 14-15 0.26194453567178044 1105 16-17 0.3451625475254687 1105 18-19 0.2998394249443592 1105 20-21 0.17950878455845043 1105 22-23 0.16625109677715244 1105 24-25 0.24778401281030682 1105 26-27 0.25966933572964024 1105 28-29 0.2789591869908463 1105 30-31 0.3035521047652239 1105 32-33 0.41416485671499714 1105 34-35 0.17420364628132745 1105 36-37 0.3392136919515494 1105 38-39 0.2860839508975488 1105 40-41 0.45958838713527683 1105 42-43 0.4916897570072507 1105 44-45 0.4134300805011648 1105 46-47 0.40781275842923037 1105 48-49 0.3269468526356789 1105 50-51 0.4290911813837468 1105 52-53 0.5086578754279145 1105 54-55 0.493062766425723 1105 56-57 0.57462579231197 1105 58-59 0.5521124989954345 1105 60-61 0.38227152552509835 1105 62-63 0.5771766452677198 1105 64-65 0.35846049862357177 1105 66-67 0.47872149884008053 1105 68-69 0.3157357820000932 1105 70-71 0.1776166833491999 1105 72-73 0.2848737331840958 1105 74-75 0.4208150584239654 1105 76-77 0.3524292233611561 1105 78-79 0.3414723453276949 1105 80-81 0.4999400092237387 1105 82-83 0.41281521643030317 1105 84-85 0.5399398966735056 1105 86-87 0.5787393798560885 1105 88-89 0.6107834976545696 1105 90-91 0.6912623053792792 1105 92-93 0.6044283342971646 1105 94-95 0.8624888371441273 1105 96-97 0.8105208886437687 1105 98-99 0.722075509775653 1105 100-101 0.406348638509062 1106 1 0.1563437096771949 1106 2 0.1990266699958525 1106 3 0.15070132456749263 1106 4 0.07854425915913765 1106 5 0.10193514530877934 1106 6 0.06394345575098725 1106 7 0.09007874231880919 1106 8 0.10538073073610832 1106 9 0.12484528972323972 1106 10-11 0.062048273826576406 1106 12-13 0.11305223348681892 1106 14-15 0.1122795176214666 1106 16-17 0.16393518938521368 1106 18-19 0.13573038322191167 1106 20-21 0.03584146836595892 1106 22-23 0.10186643943828955 1106 24-25 0.03364598108414896 1106 26-27 0.14092368335277428 1106 28-29 0.24410941206699732 1106 30-31 0.24351217507124545 1106 32-33 0.2021201770124179 1106 34-35 0.1156466465291146 1106 36-37 0.20761241189898527 1106 38-39 0.1943272549437296 1106 40-41 -0.0037984841133109626 1106 42-43 0.3448913624778669 1106 44-45 0.24123410877800922 1106 46-47 0.17199197631683205 1106 48-49 0.22942962183383742 1106 50-51 0.049767687337229916 1106 52-53 -0.10667373020415027 1106 54-55 0.10536007330351538 1106 56-57 0.051301210964822985 1106 58-59 -0.03084585367042081 1106 60-61 -0.10383643661803177 1106 62-63 -0.1931123211930199 1106 64-65 -0.06082761077193055 1106 66-67 -0.44639312749421833 1106 68-69 -0.4035315676046096 1106 70-71 -0.29132303709731744 1106 72-73 -0.026055814087509788 1106 74-75 -0.00548566618573787 1106 76-77 -0.08114093771235531 1106 78-79 -0.4253737949656724 1106 80-81 -0.5138472645156007 1106 82-83 -0.27956943248530663 1106 84-85 -0.24900340014271904 1106 86-87 -0.5042688103464137 1106 88-89 -0.39008444761398664 1106 90-91 -0.14144993057829325 1106 92-93 -0.3555152720733368 1106 94-95 -0.25547345338905814 1106 96-97 -0.3148826374672389 1106 98-99 -0.09765299497891888 1106 100-101 -0.5724890974909762 1107 1 0.07871745218604076 1107 2 0.17596376965017413 1107 3 0.08330882198917067 1107 4 0.09762854485840222 1107 5 -0.004701913177278527 1107 6 0.08472706255218299 1107 7 0.06574942425974228 1107 8 0.10198522551331024 1107 9 0.0954395981693068 1107 10-11 0.11140470370591515 1107 12-13 0.1144299217642697 1107 14-15 0.3103948193907442 1107 16-17 0.22671817506415692 1107 18-19 0.21512792785146218 1107 20-21 -0.0027719084963706564 1107 22-23 0.09532795596935273 1107 24-25 0.1855062200888895 1107 26-27 0.07628157506529476 1107 28-29 0.1405993701292445 1107 30-31 0.19236489805386014 1107 32-33 0.10995484260931931 1107 34-35 0.05406420196095496 1107 36-37 0.3070592329654076 1107 38-39 0.3163813759865377 1107 40-41 0.2731910400306461 1107 42-43 0.2985498482054396 1107 44-45 0.30290909428791934 1107 46-47 0.37764581266939246 1107 48-49 0.3336817443244584 1107 50-51 0.43149510471791785 1107 52-53 0.48780383604474054 1107 54-55 0.3898908076910814 1107 56-57 0.33658574345222547 1107 58-59 0.3210350340509365 1107 60-61 0.37002983535490586 1107 62-63 0.0980754560953514 1107 64-65 0.23697541375760522 1107 66-67 0.4378639883321682 1107 68-69 0.38945250040661605 1107 70-71 0.0474866963275673 1107 72-73 0.1503173486334184 1107 74-75 0.02675954682726811 1107 76-77 -0.035580716256255585 1107 78-79 0.04247454786871785 1107 80-81 0.032709317488869516 1107 82-83 0.1734101736316589 1107 84-85 0.07822358424231268 1107 86-87 0.15416294269271802 1107 88-89 0.36666675085117717 1107 90-91 0.2685781306400763 1107 92-93 0.17504556662992243 1107 94-95 0.5947841388893913 1107 96-97 0.4075736076439114 1107 98-99 0.6991222069273348 1107 100-101 0.5808107390354031 1108 1 -0.023737205426797914 1108 2 0.15385830714903648 1108 3 -0.0023267225429677296 1108 4 -0.004266174825048097 1108 5 0.04485768755081665 1108 6 0.07592507002814841 1108 7 0.06308837068948492 1108 8 0.033590095914547646 1108 9 0.06877013214592864 1108 10-11 0.010000004622114034 1108 12-13 0.03136663579360999 1108 14-15 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2316 70-71 -1.609048806569998 2316 72-73 -1.5851032146346995 2316 74-75 -1.854024873747104 2316 76-77 -1.803182051689987 2316 78-79 -1.870607344614431 2316 80-81 -1.903428656706705 2316 82-83 -2.1798962662111627 2316 84-85 -1.978923893298191 2316 86-87 -2.2202597059016504 2316 88-89 -2.219879146949591 2316 90-91 -2.722776669100387 2316 92-93 -2.340753714571047 2316 94-95 -2.6544227589478666 2316 96-97 -2.5600365124943565 2316 98-99 -2.3540464736774673 2316 100-101 -1.9087745277261305 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 11.0 4 24.0 5 66.0 6 157.0 7 353.0 8 851.0 9 1698.0 10 2657.0 11 4226.0 12 6243.0 13 8664.0 14 11864.0 15 15347.0 16 19627.0 17 24996.0 18 22833.0 19 16127.0 20 15395.0 21 16165.0 22 17432.0 23 19210.0 24 22646.0 25 26614.0 26 32703.0 27 39966.0 28 49044.0 29 60036.0 30 73470.0 31 90120.0 32 111705.0 33 142047.0 34 184635.0 35 248071.0 36 337147.0 37 387735.0 38 182629.0 39 6681.0 >>END_MODULE >>Per base sequence content fail #Base G A 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22.120943278081867 18-19 21.23730850931526 28.414024351565065 29.219081554074837 21.12958558504484 20-21 22.78992307368761 25.561748513368038 28.816685291011545 22.83164312193281 22-23 21.74196250259186 25.83573876223631 29.363511689747874 23.058787045423955 24-25 23.46268932258907 25.86273261126429 29.11599932611537 21.55857874003127 26-27 22.689680510804394 24.98729082147219 30.94621311950993 21.376815548213486 28-29 20.979393174376938 28.02807477360502 29.70623161119835 21.286300440819687 30-31 21.73579323846661 25.905054609866674 29.267564587969986 23.091587563696734 32-33 21.904360445750722 26.258576436090504 29.7542677837491 22.08279533440967 34-35 23.660064592183687 25.58527998472159 29.2002141704929 21.55444125260182 36-37 22.89012733422973 25.011288334296573 30.399904873331927 21.698679458141765 38-39 21.91231155578949 26.40298213922334 30.385465822833734 21.299240482153433 40-41 22.093594552742736 26.786145043296454 29.410010055936286 21.710250348024516 42-43 21.388132222513494 27.14204286961842 28.108913576637224 23.36091133123086 44-45 22.480287739097633 25.607670132289684 28.381631280963294 23.53041084764939 46-47 23.6994469349977 27.072811510008872 27.911230407070935 21.316511147922494 48-49 23.403061915852792 26.59586948751925 28.078876982246744 21.92219161438122 50-51 21.388829270599533 27.14187040964434 29.60281487247951 21.866485447276617 52-53 22.867633768457143 28.168626296362397 27.07506084647363 21.888679088706827 54-55 21.604367777049482 26.62611094552033 28.109971073526175 23.65955020390402 56-57 23.046048074959717 26.44227380361729 27.702721446368955 22.808956675054038 58-59 24.904070825594545 25.95879723447507 26.967460360225786 22.169671579704602 60-61 22.7459713861842 26.812748324608222 29.392243461851088 21.049036827356492 62-63 22.048330034014775 26.980779072672846 28.965121955045753 22.00576893826663 64-65 21.568897166955114 28.60790098575884 27.756559675288 22.066642171998048 66-67 22.34127232236744 27.003627509046034 26.924120150011312 23.73098001857521 68-69 21.850000477453506 27.00081439927027 27.769605491533838 23.379579631742384 70-71 23.372306351890366 26.945427997228848 27.196395956410065 22.485869694470725 72-73 23.0717951924334 25.733020331834638 29.0161029039794 22.179081571752562 74-75 21.76451716248076 28.86102100179329 27.356082339162352 22.018379496563593 76-77 22.34449815157537 26.81136519686973 27.269310694489285 23.57482595706562 78-79 22.721977040033288 27.01788731017328 27.674990734614347 22.58514491517909 80-81 23.574912243964846 26.923567500243845 27.207497124439524 22.294023131351786 82-83 22.985446009816645 26.520697805760662 28.30968450136009 22.184171683062605 84-85 22.227254476394712 27.55450181365775 27.95995447325713 22.258289236690405 86-87 22.269984930760618 27.99584118094819 27.17369458280397 22.56047930548722 88-89 21.757014904469433 28.054820545677195 26.199867311706992 23.988297238146384 90-91 22.490453122815076 26.545263964475048 26.895761402535452 24.06852151017443 92-93 23.476874946001374 27.39456057622787 27.244523970843016 21.88404050692774 94-95 22.816610392146742 26.797213246061784 27.56588279454772 22.820293567243752 96-97 21.146682696767364 26.910243561738017 29.073124905647337 22.869948835847282 98-99 22.065454337112616 28.001570583971674 26.5972529786075 23.335722100308207 100-101 21.28762755479041 26.89028457774425 27.29041590103099 24.531671966434352 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 348.0 1 266.5 2 253.0 3 346.0 4 311.5 5 281.0 6 482.5 7 996.0 8 1555.5 9 1927.0 10 1991.5 11 1879.5 12 1981.0 13 2217.5 14 2476.5 15 2774.0 16 3073.5 17 3303.0 18 3559.0 19 3896.0 20 4336.5 21 4946.5 22 5658.5 23 6657.0 24 8020.0 25 9741.5 26 12056.0 27 15021.0 28 17703.0 29 20752.5 30 24584.5 31 28622.0 32 33969.5 33 37754.5 34 40508.0 35 43595.0 36 47674.5 37 52114.5 38 54068.0 39 54575.5 40 55113.5 41 57526.0 42 60234.5 43 62792.0 44 65089.0 45 68151.0 46 72854.0 47 101281.5 48 161624.0 49 156573.0 50 98764.5 51 74929.0 52 67439.0 53 65589.0 54 64634.5 55 60403.0 56 54566.5 57 49295.0 58 44883.0 59 39641.0 60 33607.5 61 27743.5 62 23210.5 63 18643.5 64 13835.0 65 10662.5 66 8068.0 67 6327.0 68 4664.5 69 3079.5 70 2336.5 71 1861.0 72 1634.0 73 1497.0 74 1249.0 75 1098.5 76 806.0 77 472.5 78 302.0 79 206.0 80 141.5 81 104.5 82 74.5 83 40.5 84 24.0 85 13.0 86 5.5 87 2.0 88 1.5 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012004370682234759 2 0.00950346012343585 3 0.005092763319735958 4 4.5471101069071053E-4 5 0.005092763319735958 6 0.0 7 5.001821117597816E-4 8 0.0026373238620061214 9 9.094220213814212E-5 10-11 0.0 12-13 0.0 14-15 5.001821117597816E-4 16-17 0.004638052309045248 18-19 0.0017506373911592356 20-21 7.502731676396724E-4 22-23 3.182977074834974E-4 24-25 4.31975460156175E-4 26-27 4.547110106907106E-5 28-29 2.273555053453553E-5 30-31 3.86504359087104E-4 32-33 0.0035240103328530066 34-35 7.502731676396724E-4 36-37 0.0011822486277958475 38-39 0.004842672263856068 40-41 2.273555053453553E-5 42-43 0.00193252179543552 44-45 0.005615680982030276 46-47 0.0 48-49 0.0024099683566607658 50-51 0.0010458353245886343 52-53 7.502731676396724E-4 54-55 1.1367775267267763E-4 56-57 0.011731544075820333 58-59 0.002296290603988088 60-61 0.003933250242474646 62-63 3.410332580180329E-4 64-65 0.0036831591865947554 66-67 0.0014323396836757383 68-69 0.001455075234210274 70-71 0.005160969971339565 72-73 0.0027964727157478698 74-75 0.007843764934414759 76-77 0.0013186619310030607 78-79 0.0070025495646369425 80-81 0.0028419438168169413 82-83 0.0035012747823184713 84-85 0.002364497255591695 86-87 7.73008718174208E-4 88-89 0.0027282660641442633 90-91 0.008253004844036398 92-93 0.003137505973765903 94-95 4.31975460156175E-4 96-97 1.3641330320721319E-4 98-99 7.73008718174208E-4 100-101 0.0020916706491772687 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2199199.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.12785460868556 #Duplication Level Percentage of deduplicated Percentage of total 1 75.47227182747837 35.56846253672596 2 13.917239064462883 13.117792383686517 3 4.570308561227193 6.461665121710382 4 2.012503822201181 3.7937995012848487 5 1.0427237410168317 2.4570666431832975 6 0.6380297298523375 1.8041383406699931 7 0.4245981202009969 1.4007278933167644 8 0.30298073517917073 1.1423065629405296 9 0.223984382372565 0.9500313066363427 >10 1.1933677009127286 10.549288812570621 >50 0.12046779967515653 3.949607925726781 >100 0.07111931108583047 6.143132124253113 >500 0.006166047013178024 1.922587667300195 >1k 0.0028903345374271974 2.9492875311027587 >5k 8.671003612281594E-4 3.3334145808006928 >10k+ 4.81722422904533E-4 4.456691068091143 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 40225 1.8290750405033833 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 18526 0.8423976184056103 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 14399 0.6547383842935541 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 14092 0.6407787562653493 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 10912 0.4961806548657034 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 9952 0.45252839783939514 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8900 0.40469279951473247 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 8896 0.4045109151104561 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8565 0.3894599806565936 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 8493 0.38618606137962047 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8166 0.37131701133003425 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 7817 0.35544759705692847 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6483 0.29478914823078767 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 6143 0.2793289738673035 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4745 0.21576037457274216 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4663 0.21203174428507834 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3672 0.1669698831256289 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3529 0.16046751567275175 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3524 0.16024016016740641 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3481 0.15828490282143634 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3165 0.1439160348836099 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3133 0.14246095964939962 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2605 0.11845221828493009 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2516 0.11440529028978279 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2461 0.11190437973098387 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2384 0.1084031049486654 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2202 0.10012736455409448 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.547110106907106E-5 2 0.0 0.0 0.0 0.0 4.547110106907106E-5 3 0.0 0.0 0.0 0.0 4.547110106907106E-5 4 0.0 0.0 0.0 0.0 4.547110106907106E-5 5 0.0 0.0 0.0 0.0 4.547110106907106E-5 6 0.0 0.0 0.0 0.0 4.547110106907106E-5 7 0.0 0.0 0.0 0.0 4.547110106907106E-5 8 0.0 0.0 0.0 0.0 4.547110106907106E-5 9 0.0 0.0 0.0 0.0 4.547110106907106E-5 10-11 0.0 0.0 0.0 0.0 4.547110106907106E-5 12-13 0.0 0.0 0.0 0.0 4.547110106907106E-5 14-15 0.0 0.0 0.0 0.0 4.547110106907106E-5 16-17 0.0 0.0 0.0 2.273555053453553E-5 4.547110106907106E-5 18-19 0.0 0.0 0.0 4.547110106907106E-5 4.547110106907106E-5 20-21 0.0 0.0 0.0 6.820665160360659E-5 4.547110106907106E-5 22-23 0.0 0.0 0.0 1.1367775267267765E-4 6.820665160360659E-5 24-25 0.0 0.0 0.0 1.8188440427628424E-4 9.094220213814212E-5 26-27 0.0 0.0 0.0 3.4103325801803296E-4 9.094220213814212E-5 28-29 0.0 0.0 0.0 8.866864708468856E-4 9.094220213814212E-5 30-31 0.0 0.0 0.0 0.002159877300780875 9.094220213814212E-5 32-33 0.0 0.0 0.0 0.003933250242474646 9.094220213814212E-5 34-35 0.0 0.0 0.0 0.006229540846462735 1.3641330320721316E-4 36-37 0.0 0.0 0.0 0.008207533742967326 1.3641330320721316E-4 38-39 0.0 0.0 0.0 0.01114041976192241 1.3641330320721316E-4 40-41 0.0 0.0 0.0 0.015642058767760446 1.3641330320721316E-4 42-43 0.0 0.0 0.0 0.02125773974979072 1.3641330320721316E-4 44-45 0.0 0.0 0.0 0.026918891832890066 1.3641330320721316E-4 46-47 0.0 0.0 0.0 0.03358040813950897 1.3641330320721316E-4 48-49 0.0 0.0 0.0 0.03971900678383357 1.8188440427628424E-4 50-51 0.0 0.0 0.0 0.050677542141479695 1.8188440427628424E-4 52-53 0.0 0.0 0.0 0.07495911011236364 2.273555053453553E-4 54-55 0.0 0.0 0.0 0.11215447078686377 2.728266064144263E-4 56-57 0.0 2.273555053453553E-5 0.0 0.1447572502533877 2.728266064144263E-4 58-59 0.0 4.547110106907106E-5 0.0 0.187818382965798 2.728266064144263E-4 60-61 0.0 4.547110106907106E-5 0.0 0.24101957121661116 2.728266064144263E-4 62-63 0.0 4.547110106907106E-5 0.0 0.2730539619197717 2.728266064144263E-4 64-65 0.0 4.547110106907106E-5 0.0 0.2987451340237969 2.728266064144263E-4 66-67 0.0 4.547110106907106E-5 0.0 0.3781149409398604 2.728266064144263E-4 68-69 0.0 4.547110106907106E-5 0.0 0.4677157455964649 2.728266064144263E-4 70-71 0.0 4.547110106907106E-5 0.0 0.5073665457286949 3.182977074834974E-4 72-73 0.0 4.547110106907106E-5 0.0 0.5745500975582474 3.182977074834974E-4 74-75 0.0 4.547110106907106E-5 0.0 0.6787925967590926 3.182977074834974E-4 76-77 0.0 4.547110106907106E-5 0.0 0.780397772097932 3.182977074834974E-4 78-79 0.0 4.547110106907106E-5 0.0 0.8839354692322068 3.182977074834974E-4 80-81 0.0 4.547110106907106E-5 0.0 1.0219857320779064 3.182977074834974E-4 82-83 0.0 4.547110106907106E-5 0.0 1.1789747085188744 3.182977074834974E-4 84-85 0.0 4.547110106907106E-5 0.0 1.345330731780071 3.182977074834974E-4 86-87 0.0 4.547110106907106E-5 0.0 1.5750052632799487 3.182977074834974E-4 88-89 0.0 4.547110106907106E-5 0.0 1.8646107059888624 3.182977074834974E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 380 0.0 29.373547 94-95 GTACATG 9750 0.0 23.389307 1 TACATGG 10085 0.0 22.561668 2 GAGTACT 10055 0.0 21.587692 12-13 ACATGGG 9930 0.0 21.573917 3 GTACTTT 10380 0.0 20.981369 14-15 CATGGGG 4425 0.0 20.609617 4 TAAGGTG 855 0.0 20.554539 5 GTATAGG 790 0.0 19.244368 1 AGTACTT 10365 0.0 18.94865 12-13 GTAAGGT 805 0.0 18.881481 4 GTACCTG 1840 0.0 18.332481 1 ACTTTTT 11970 0.0 18.313002 16-17 TACCTGG 1790 0.0 18.04544 2 GGGCGTA 430 3.6379788E-12 17.672739 9 TACTTTT 11350 0.0 17.095745 14-15 AGAGTAC 18185 0.0 16.206186 10-11 ATGGGGA 2570 0.0 16.078968 5 CATGGGA 5145 0.0 15.786749 4 TAGCCCT 1330 0.0 15.713864 4 >>END_MODULE