##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936426_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2298298 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.26940675230105 31.0 31.0 34.0 28.0 34.0 2 31.284618008630733 31.0 31.0 34.0 28.0 34.0 3 31.33835690584946 31.0 31.0 34.0 28.0 34.0 4 34.957780931802574 37.0 35.0 37.0 32.0 37.0 5 34.88460025636362 37.0 35.0 37.0 32.0 37.0 6 35.02939958177747 37.0 35.0 37.0 32.0 37.0 7 34.86444534172679 37.0 35.0 37.0 32.0 37.0 8 34.85557616984394 37.0 35.0 37.0 32.0 37.0 9 36.32644548270068 39.0 37.0 39.0 32.0 39.0 10-11 36.19314249066048 39.0 35.0 39.0 31.5 39.0 12-13 36.105268550901584 39.0 35.0 39.0 31.0 39.0 14-15 37.10087682276189 39.0 36.0 41.0 31.0 41.0 16-17 37.067477977181376 39.0 36.0 41.0 31.0 41.0 18-19 37.055552848238136 39.0 36.0 41.0 31.0 41.0 20-21 36.94557559550589 39.0 36.0 41.0 31.0 41.0 22-23 36.84270512353054 39.0 36.0 41.0 30.5 41.0 24-25 36.65286290115555 39.0 36.0 41.0 30.0 41.0 26-27 36.43117667943844 39.0 35.5 40.0 30.0 41.0 28-29 36.58611263639441 39.0 36.0 41.0 30.0 41.0 30-31 36.55738637896391 39.0 36.0 41.0 30.0 41.0 32-33 36.39613444383626 39.0 35.0 40.0 30.0 41.0 34-35 36.175722643451806 39.0 35.0 40.0 30.0 41.0 36-37 35.910964330996244 38.0 35.0 40.0 28.5 41.0 38-39 35.71443759686515 38.0 35.0 40.0 27.5 41.0 40-41 35.62932265528665 38.0 35.0 40.0 27.5 41.0 42-43 35.352052257801205 38.0 34.0 40.0 26.5 41.0 44-45 35.05732633453103 38.0 34.0 40.0 25.5 41.0 46-47 34.677541815726244 38.0 33.5 40.0 24.0 41.0 48-49 34.370366897591175 38.0 33.0 40.0 23.5 41.0 50-51 33.626498391418345 37.5 32.0 39.5 23.0 40.5 52-53 33.84512952628423 37.5 33.0 39.5 23.0 40.5 54-55 34.21180456146244 38.0 33.0 40.0 23.0 41.0 56-57 34.06579999634512 38.0 33.0 40.0 22.5 41.0 58-59 33.81975618479414 38.0 33.0 40.0 22.0 41.0 60-61 33.40869917652106 37.0 32.5 40.0 20.5 41.0 62-63 33.12874962254678 36.5 32.0 40.0 20.0 41.0 64-65 32.82558571603856 36.0 32.0 39.0 19.5 41.0 66-67 32.35832254999134 36.0 31.0 39.0 18.0 40.5 68-69 31.960841022356547 35.0 31.0 39.0 15.5 40.0 70-71 31.49353978465804 35.0 31.0 38.0 12.5 40.0 72-73 31.058664933790133 35.0 30.0 37.0 9.5 39.5 74-75 30.66029753321806 35.0 30.0 37.0 7.5 39.0 76-77 30.249301222034738 34.0 29.5 36.0 7.0 39.0 78-79 29.76201606580174 34.0 29.0 36.0 2.0 37.5 80-81 29.27623397836138 34.0 29.0 35.0 2.0 37.0 82-83 28.841746805679684 34.0 28.5 35.0 2.0 37.0 84-85 28.444952743290905 34.0 27.5 35.0 2.0 36.0 86-87 28.182772207955626 34.0 27.0 35.0 2.0 36.0 88-89 27.74599138144836 33.5 26.5 35.0 2.0 35.5 90-91 27.331358248582212 33.0 26.0 35.0 2.0 35.0 92-93 26.94672514182234 33.0 25.0 35.0 2.0 35.0 94-95 26.48437691717958 33.0 25.0 35.0 2.0 35.0 96-97 25.87058640785486 33.0 23.5 35.0 2.0 35.0 98-99 25.21073442173295 32.0 19.0 35.0 2.0 35.0 100-101 23.06735962873396 30.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3907368981101733 1101 2 0.4842046250124774 1101 3 0.3988515307966587 1101 4 0.3039206750208834 1101 5 0.47951354177670424 1101 6 0.3259852918332413 1101 7 0.3212754347378137 1101 8 0.40038425086111573 1101 9 0.5497242999146152 1101 10-11 0.3757308284352163 1101 12-13 0.5163651490475303 1101 14-15 0.621142965489625 1101 16-17 0.78604274365604 1101 18-19 0.8013976288051339 1101 20-21 0.6591902335225086 1101 22-23 0.6797417625351088 1101 24-25 0.54454989965528 1101 26-27 0.5461823822969407 1101 28-29 0.5888884693194782 1101 30-31 0.47178912745162904 1101 32-33 0.4492050900495528 1101 34-35 0.31311452668742135 1101 36-37 0.490351627736743 1101 38-39 0.5680013553928944 1101 40-41 0.6120896153900972 1101 42-43 0.44947563536091195 1101 44-45 0.3108936420601438 1101 46-47 0.380126121057458 1101 48-49 0.44910046988634633 1101 50-51 0.5826992815890861 1101 52-53 0.6632602557793135 1101 54-55 0.7147133650467552 1101 56-57 0.6499389255360484 1101 58-59 0.5642627687174055 1101 60-61 0.6176027876728654 1101 62-63 0.7379090868661606 1101 64-65 0.8157750609940209 1101 66-67 0.5994855169220372 1101 68-69 0.5063908109981377 1101 70-71 0.5671650319866153 1101 72-73 0.4809948832325439 1101 74-75 0.29297377519691636 1101 76-77 0.39156607438798474 1101 78-79 0.46881684895530995 1101 80-81 0.4004095048857934 1101 82-83 0.4139937864798391 1101 84-85 0.4960652917403756 1101 86-87 0.4611820170253651 1101 88-89 0.6050839073578338 1101 90-91 0.7161870077596362 1101 92-93 0.5830824985439804 1101 94-95 0.7611787862296708 1101 96-97 0.4416776206994477 1101 98-99 0.5444043484004375 1101 100-101 0.6059119991284518 1102 1 0.30434930593795784 1102 2 0.3684702065365286 1102 3 0.3271775450112919 1102 4 0.3818158049459157 1102 5 0.4376289882771047 1102 6 0.3661695594004044 1102 7 0.2658222215849193 1102 8 0.09360033737873152 1102 9 0.3709012346984437 1102 10-11 0.40169581836608614 1102 12-13 0.5440658141735071 1102 14-15 0.5995507112864686 1102 16-17 0.4310014871418488 1102 18-19 0.2731855007607038 1102 20-21 0.14948508853221654 1102 22-23 0.09783243334899083 1102 24-25 0.2637586790107491 1102 26-27 0.3690488741417255 1102 28-29 0.29546328190050986 1102 30-31 0.29419059477693565 1102 32-33 0.3562480657452909 1102 34-35 0.12638316225680768 1102 36-37 0.03397217669627395 1102 38-39 0.0460723427309091 1102 40-41 -0.19510952143216542 1102 42-43 -0.023161098501574884 1102 44-45 0.05582817352553349 1102 46-47 0.2143287064913011 1102 48-49 0.17936847389349708 1102 50-51 0.25681317288351835 1102 52-53 0.25264664759898636 1102 54-55 0.12354453936817578 1102 56-57 0.28940368615731416 1102 58-59 0.23734844980537417 1102 60-61 -0.11541468981892677 1102 62-63 0.07419883813569328 1102 64-65 0.25123817673516413 1102 66-67 0.16632318365190457 1102 68-69 0.3599642938396279 1102 70-71 0.3347659858633598 1102 72-73 0.07350818062789344 1102 74-75 0.08673476028026172 1102 76-77 0.19018147348919356 1102 78-79 0.11561967727799072 1102 80-81 0.2765640595639631 1102 82-83 0.1401042727186983 1102 84-85 0.10291172091573486 1102 86-87 0.07253521275585229 1102 88-89 -0.19300978035065697 1102 90-91 -0.3407996333541732 1102 92-93 -0.17167039152575825 1102 94-95 -0.030071803057595048 1102 96-97 -0.13855768426952508 1102 98-99 -0.3635925360633898 1102 100-101 0.1386371388920189 1103 1 0.1936613012371282 1103 2 0.3475881128323195 1103 3 0.39425921202093406 1103 4 0.20497201209980886 1103 5 0.39410524362938304 1103 6 0.20729587438083286 1103 7 0.1839760795633154 1103 8 0.016109932427987417 1103 9 0.3855045525524261 1103 10-11 0.3068039269265128 1103 12-13 0.43045141242607343 1103 14-15 0.4659359314395459 1103 16-17 0.5570449022603725 1103 18-19 0.4114357183228634 1103 20-21 0.2646415628350809 1103 22-23 0.26797921652815404 1103 24-25 0.4382926558983371 1103 26-27 0.33747160114398156 1103 28-29 0.5729143118187068 1103 30-31 0.29798483861515024 1103 32-33 0.5143431602453745 1103 34-35 0.30784844652563237 1103 36-37 0.47626667962560987 1103 38-39 0.4609092299927937 1103 40-41 0.43998147347775074 1103 42-43 0.42760438250294897 1103 44-45 0.4430667425243442 1103 46-47 0.6017538458922331 1103 48-49 0.5122941364353721 1103 50-51 -0.03083561856175976 1103 52-53 0.4366037469436961 1103 54-55 0.7244172983539272 1103 56-57 0.7268596778564813 1103 58-59 0.6567511982787195 1103 60-61 0.8024665181407542 1103 62-63 0.6952865156874921 1103 64-65 0.7151719795730074 1103 66-67 0.5009869416384802 1103 68-69 0.5018193955793784 1103 70-71 0.6580149358624929 1103 72-73 0.45357703812281613 1103 74-75 0.37104202003832754 1103 76-77 0.422985307417985 1103 78-79 0.5928744363728349 1103 80-81 0.5961714001485419 1103 82-83 0.5660827755934967 1103 84-85 0.5124373176311039 1103 86-87 0.4885975917330221 1103 88-89 0.4530292091443755 1103 90-91 0.5140957012700724 1103 92-93 0.5823420343109582 1103 94-95 0.5003981343142527 1103 96-97 0.6424929612268784 1103 98-99 0.533584238462872 1103 100-101 0.5734025033675287 1104 1 0.1615183834624112 1104 2 0.14300167666371877 1104 3 0.10404422358342913 1104 4 0.15245815468895785 1104 5 0.2248174617973504 1104 6 0.15247876431339336 1104 7 0.4339869932975233 1104 8 0.34705664430176597 1104 9 0.36279807379601436 1104 10-11 0.40259058888813826 1104 12-13 0.2912407452209962 1104 14-15 0.2842950909122379 1104 16-17 0.12718193935130984 1104 18-19 0.4034338290342063 1104 20-21 0.31826187584098165 1104 22-23 0.29430246132685056 1104 24-25 0.25900145139799946 1104 26-27 0.14714888505506707 1104 28-29 0.17869355850427127 1104 30-31 0.23328481169460957 1104 32-33 0.02734641981399477 1104 34-35 0.04965488477996871 1104 36-37 0.11951732323276332 1104 38-39 0.12452505320970886 1104 40-41 0.09303697042251002 1104 42-43 0.1298074561742908 1104 44-45 -0.027538023867172967 1104 46-47 0.17973649366116717 1104 48-49 0.11173113932993317 1104 50-51 0.018052681187590736 1104 52-53 0.0640385707293305 1104 54-55 0.3708013205257856 1104 56-57 0.23980996034030966 1104 58-59 0.22429273716375775 1104 60-61 0.14775387676242246 1104 62-63 -0.06121162776505429 1104 64-65 0.013660791176498321 1104 66-67 0.07919870203593149 1104 68-69 0.0755928282762035 1104 70-71 0.07395743079063877 1104 72-73 -0.13209433539488202 1104 74-75 -0.2644629796645752 1104 76-77 -0.1330266291756459 1104 78-79 -0.004538144616166306 1104 80-81 0.1089647977031376 1104 82-83 -0.07355799884508585 1104 84-85 0.04804610391472153 1104 86-87 -0.023406757996372818 1104 88-89 -0.0864229547459594 1104 90-91 -0.22856701038057992 1104 92-93 -0.3352918620498002 1104 94-95 -0.2350164178164107 1104 96-97 -0.20739362688515328 1104 98-99 0.07650310455013809 1104 100-101 0.09699837567198699 1105 1 0.10613037500926836 1105 2 0.18602669129092675 1105 3 0.08671203399559957 1105 4 0.050145193721675696 1105 5 0.14865386293966054 1105 6 0.14616518702106873 1105 7 0.25429129928308924 1105 8 -0.11112020494368835 1105 9 0.13731546605626477 1105 10-11 0.26263749807210246 1105 12-13 0.06497969672354031 1105 14-15 0.061342134053795405 1105 16-17 0.13063307426642723 1105 18-19 0.008879775645795007 1105 20-21 -0.3549986728685184 1105 22-23 -0.3135303181549318 1105 24-25 -0.2502628721522271 1105 26-27 -0.332034901751733 1105 28-29 -0.2583184424136107 1105 30-31 -0.28585933447894263 1105 32-33 -0.4418530964969989 1105 34-35 -0.40985861189752626 1105 36-37 -0.4233953592469035 1105 38-39 -0.5656894651496884 1105 40-41 -0.6107768711807466 1105 42-43 -0.6125729551415731 1105 44-45 -0.41007133605562984 1105 46-47 -0.48317733316471134 1105 48-49 -0.3378799331357101 1105 50-51 -0.4238489969465675 1105 52-53 -0.3854413975053035 1105 54-55 -0.21250520627233982 1105 56-57 -0.07786527780985608 1105 58-59 -0.23978567968583064 1105 60-61 -0.24159460278892908 1105 62-63 -0.3205277622656766 1105 64-65 -0.3947248336725764 1105 66-67 -0.729189385710491 1105 68-69 -0.5187375670008016 1105 70-71 -0.28948519811801177 1105 72-73 -0.4382092740086705 1105 74-75 -0.5020994602203039 1105 76-77 -0.13847721624627596 1105 78-79 -0.19473366562708705 1105 80-81 -0.1747436318934419 1105 82-83 -0.17115586297244434 1105 84-85 -0.26779435079619773 1105 86-87 -0.2750537455124906 1105 88-89 -0.19447724481279138 1105 90-91 -0.25864311691230313 1105 92-93 -0.3289143094969731 1105 94-95 -0.3435780797555168 1105 96-97 -0.30632686194287473 1105 98-99 -0.6031976954710068 1105 100-101 -0.15944007351964373 1106 1 -0.18468735564512784 1106 2 -0.10073488601453207 1106 3 -0.07895564478566186 1106 4 0.09303045662100828 1106 5 0.1126094264617663 1106 6 0.16581919109004417 1106 7 0.22598090902449286 1106 8 0.2642809141158722 1106 9 0.24777642782464682 1106 10-11 0.22962940538003096 1106 12-13 0.22567484201358212 1106 14-15 0.17059249632080764 1106 16-17 0.08695681393631105 1106 18-19 -0.011543574326772443 1106 20-21 0.15061986071805222 1106 22-23 0.1698865734763828 1106 24-25 -0.12507102604192966 1106 26-27 -0.13034277832082353 1106 28-29 -0.18673577763718185 1106 30-31 -0.10457607637523836 1106 32-33 -0.18358296751726755 1106 34-35 -0.21094414553902396 1106 36-37 -0.21787280152408073 1106 38-39 -0.12197393030717762 1106 40-41 -0.19025925030788926 1106 42-43 -0.13583559737023165 1106 44-45 -0.12077109832639366 1106 46-47 -0.19641801908509393 1106 48-49 -0.19169455502309773 1106 50-51 0.10241438098136513 1106 52-53 0.03178534211568973 1106 54-55 0.16216541892720215 1106 56-57 0.06716962716785702 1106 58-59 0.23668341038271734 1106 60-61 0.11783161212579785 1106 62-63 0.17790231341055573 1106 64-65 0.027867788090482293 1106 66-67 0.07943819715827516 1106 68-69 0.09448460573221595 1106 70-71 0.14973021390678198 1106 72-73 0.24189740842211904 1106 74-75 0.2056668595994573 1106 76-77 0.006356789717774092 1106 78-79 0.1781554990735934 1106 80-81 0.10364197290387267 1106 82-83 0.021768372675840197 1106 84-85 0.11876115225251382 1106 86-87 0.15203530246608565 1106 88-89 0.2689247336690599 1106 90-91 0.2280816301646631 1106 92-93 -0.09734888748381465 1106 94-95 0.1416033736217308 1106 96-97 0.2253905130531244 1106 98-99 0.3533430121159924 1106 100-101 0.5940302815412615 1107 1 0.4164818390099789 1107 2 0.4221908091822897 1107 3 0.48365721184127963 1107 4 0.4317116893163231 1107 5 0.42128283443095427 1107 6 0.41417298909173184 1107 7 0.49873807165841555 1107 8 0.6117807636403683 1107 9 0.7212227682604251 1107 10-11 0.7918002402181159 1107 12-13 0.7018101796806135 1107 14-15 0.7823547901693715 1107 16-17 0.7749413450546427 1107 18-19 0.7905758731271817 1107 20-21 0.8700816098452933 1107 22-23 0.8725308042456135 1107 24-25 0.8011346589225639 1107 26-27 0.8583219020692141 1107 28-29 0.6772415156094738 1107 30-31 0.687557816726958 1107 32-33 0.687837972653675 1107 34-35 0.632568855544406 1107 36-37 0.9739985118261032 1107 38-39 0.9005232858960284 1107 40-41 0.8196672424090963 1107 42-43 1.047515040645898 1107 44-45 0.7849529897920675 1107 46-47 0.7853432214349567 1107 48-49 0.9126380108851748 1107 50-51 0.7331452404974428 1107 52-53 0.5868608808180014 1107 54-55 0.9369310533319251 1107 56-57 1.0383227209441799 1107 58-59 1.029550495371275 1107 60-61 1.030971885389306 1107 62-63 1.0096823633684195 1107 64-65 0.8045576279845434 1107 66-67 0.7887631640536696 1107 68-69 0.8893846147607469 1107 70-71 0.8102360373087301 1107 72-73 0.6790584623525362 1107 74-75 0.5185543340186243 1107 76-77 0.7395230952432392 1107 78-79 0.833718954593035 1107 80-81 0.771332249176897 1107 82-83 0.7941203800190415 1107 84-85 1.0166702951440527 1107 86-87 1.1253830814476693 1107 88-89 1.122436079521954 1107 90-91 1.0111219124567086 1107 92-93 0.9633032733444011 1107 94-95 0.9956891597057549 1107 96-97 1.2899531560048523 1107 98-99 1.3770707417416084 1107 100-101 1.3284812716700678 1108 1 0.24337057527371542 1108 2 0.18002047951195976 1108 3 0.05697451953358623 1108 4 0.28466498601962087 1108 5 0.4184325597056784 1108 6 0.3396880989818385 1108 7 0.5077353244056653 1108 8 0.3891502691348734 1108 9 0.4917241418867988 1108 10-11 0.2880399380203116 1108 12-13 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-4.316928994387865 2316 94-95 -4.316364210311324 2316 96-97 -4.641375495182668 2316 98-99 -5.017631531791309 2316 100-101 -5.48623536250221 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5394.0 3 2271.0 4 2161.0 5 2525.0 6 2836.0 7 3382.0 8 4006.0 9 5103.0 10 6548.0 11 8301.0 12 10207.0 13 12213.0 14 13931.0 15 16480.0 16 19542.0 17 23680.0 18 21701.0 19 16377.0 20 17074.0 21 18358.0 22 20244.0 23 22799.0 24 26364.0 25 30118.0 26 35386.0 27 41191.0 28 48235.0 29 58089.0 30 70211.0 31 85319.0 32 105046.0 33 132495.0 34 172383.0 35 229033.0 36 306517.0 37 377260.0 38 282647.0 39 42854.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.89649939925422 18.19073907180098 13.984952852008528 21.927808676936277 2 15.338410774979232 21.067263555424628 38.50289299298259 25.091432676613557 3 21.985493100976946 23.710542587961513 29.623220089474735 24.68074422158681 4 12.424966931260112 15.855280138690663 38.50598501754308 33.21376791250614 5 13.12078132495453 38.51793423894732 33.870633538014985 14.490650898083166 6 31.054857905121473 36.21473314653213 16.81197745150454 15.918431496841853 7 28.248561997743764 33.196830475898665 20.408620701661537 18.145986824696035 8 26.046222362264487 32.011442652474045 19.470440032049176 22.471894953212296 9 25.288956466793504 18.370059236075694 21.763992194693994 34.57699210243681 10-11 25.74928968152223 25.4643134342587 26.556747775235078 22.22964910898399 12-13 28.106220330420978 23.33360104105657 26.557193796232635 22.00298483228982 14-15 23.355303241304878 25.181648852835707 27.233575879956827 24.229472025902588 16-17 21.76696411940122 28.63025365882531 27.356867554614723 22.245914667158743 18-19 21.354783602187098 28.592149288648294 28.622507548760918 21.43055956040369 20-21 23.03351531883586 25.91579748623684 27.968841157317254 23.081846037610045 22-23 21.955395026001437 26.05929197763224 28.72525511869275 23.260057877673578 24-25 23.652056127306366 26.04849264904247 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22.278907087752305 50-51 21.29696689378831 27.45187706583409 29.178013139972098 22.073142900405504 52-53 22.70130752781931 28.21985333741467 26.726228897972536 22.35261023679348 54-55 21.57760789891739 26.70868869599724 27.857437803466045 23.856265601619327 56-57 23.080603168919016 26.601032055776873 27.240720407043945 23.07764436826016 58-59 24.55689813465145 26.196072629806416 26.85133776863064 22.395691466911494 60-61 22.72227787984697 26.904975574098426 28.97868077288317 21.39406577317143 62-63 21.989087420293135 27.293654126047258 28.53034789286701 22.186910560792597 64-65 21.525746308109824 28.702213415379692 27.381513375827222 22.39052690068327 66-67 22.249351837166614 27.234521923752947 26.52971758875811 23.986408650322325 68-69 21.80018937086404 27.25134630848653 27.507793272460813 23.44067104818862 70-71 23.370206592366056 27.14113953905308 26.718107336126423 22.770546532454443 72-73 22.807837028447356 26.09944554252962 28.760406275801525 22.3323111532215 74-75 21.612809885890197 29.083096737699748 27.05966561150453 22.244427764905524 76-77 22.23983246171713 27.046006454557673 26.951368036407292 23.762793047317903 78-79 22.603423762726596 27.311886412261323 27.411233463830165 22.673456361181916 80-81 23.535632661134752 27.138100022260826 26.814219161848385 22.512048154756037 82-83 22.832250094748023 26.816214073529686 28.13842218326422 22.213113648458076 84-85 22.312038393639234 27.838151612056798 27.491798620505993 22.358011373797968 86-87 22.33839263898985 28.288568620672173 26.960411138116413 22.412627602221566 88-89 21.841902812515773 28.155247076351646 25.90831518835101 24.09453492278157 90-91 22.488540064734384 26.909938442284307 26.662484780283428 23.939036712697877 92-93 23.54996585006361 27.62006384373581 27.042528474780507 21.787441831420075 94-95 22.733638581845437 27.099127441797727 27.307623271554775 22.859610704802062 96-97 21.103773478177253 27.40692925500762 28.757770218231848 22.73152704858328 98-99 22.067730879112247 28.2365116741098 26.398914070709438 23.296843376068516 100-101 21.4540793784766 26.96736592982354 27.361237405449735 24.217317286250125 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 293.0 1 224.5 2 188.0 3 265.5 4 287.0 5 292.5 6 463.5 7 903.0 8 1461.0 9 1868.5 10 1862.0 11 1750.0 12 1909.0 13 2139.0 14 2349.5 15 2516.0 16 2720.5 17 2935.0 18 3134.0 19 3471.0 20 3840.0 21 4354.0 22 5253.0 23 6377.0 24 7744.5 25 9645.5 26 12186.5 27 15143.0 28 17861.0 29 20911.5 30 24918.0 31 29450.5 32 35155.0 33 39001.0 34 42000.5 35 45608.0 36 49726.5 37 54354.5 38 56598.5 39 57144.5 40 57639.5 41 60352.5 42 63388.5 43 66310.5 44 69414.0 45 73498.0 46 77986.0 47 104890.0 48 163299.0 49 158907.0 50 103340.5 51 79968.0 52 72215.0 53 70206.0 54 69358.5 55 64564.0 56 58314.5 57 52335.5 58 47412.5 59 42256.5 60 35810.5 61 29678.0 62 24920.0 63 19914.5 64 15034.5 65 11623.5 66 8777.0 67 7042.0 68 5258.5 69 3565.5 70 2689.5 71 2122.5 72 1800.0 73 1598.0 74 1335.5 75 1134.0 76 816.0 77 453.0 78 272.5 79 199.0 80 145.0 81 102.0 82 63.0 83 33.0 84 23.0 85 14.0 86 9.5 87 12.5 88 10.0 89 4.5 90 3.0 91 3.5 92 4.0 93 4.0 94 3.5 95 2.5 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013966857213468402 2 0.006918162918820797 3 0.009528790435356947 4 0.035286981931846956 5 0.046643211628779206 6 0.06013145379754932 7 0.028977965433551262 8 0.025192555534573842 9 0.016272911519742 10-11 0.047382889425131115 12-13 0.044228381175983274 14-15 0.01992779004289261 16-17 0.02462691957265768 18-19 0.032001942306872304 20-21 0.0255623944327498 22-23 0.015141639595909667 24-25 0.004089983109239968 26-27 0.003480836688714866 28-29 0.0017186631150529655 30-31 0.00750555411004143 32-33 0.0018056840322708367 34-35 0.002327809535578067 36-37 0.006852897230907393 38-39 0.006744121084385054 40-41 0.004655619071156134 42-43 0.001022495777309992 44-45 0.0015881317392261577 46-47 0.0028716902681897648 48-49 7.179225670474412E-4 50-51 0.0018927049494887084 52-53 0.0012835585289636068 54-55 0.0025671170579272136 56-57 0.0030239768733210403 58-59 0.0029587111854076364 60-61 8.919644014831846E-4 62-63 0.0014358451340948824 64-65 0.0036983889817595456 66-67 0.0031109977905389116 68-69 0.007048694294647604 70-71 0.010703572817798214 72-73 0.00459035338324273 74-75 0.0031762634784523154 76-77 0.003350305312888059 78-79 0.03465608028201739 80-81 0.02347389241952088 82-83 0.004220514485066776 84-85 0.00909368584926759 86-87 0.00824523190639334 88-89 0.006178485122468888 90-91 0.012705053913809262 92-93 0.014945842532169457 94-95 0.007418533192823558 96-97 0.0043510458608935825 98-99 0.02360442379534769 100-101 0.04242269714371243 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2298298.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.65355913580801 #Duplication Level Percentage of deduplicated Percentage of total 1 77.20138519715024 38.33323545252993 2 12.96105830163787 12.871253496860627 3 4.288970328479781 6.388879255106901 4 1.8956124717759406 3.7649562386360738 5 0.9637738210173351 2.3927400207713942 6 0.5915013927418371 1.7622089630051765 7 0.3563859729364508 1.2387082387660773 8 0.25778642037834404 1.0240010614931494 9 0.19704530596327208 0.880560067687264 >10 1.1075487833608293 10.354067934937026 >50 0.1047371982216447 3.6157174108023686 >100 0.06547443865471406 5.903792270131196 >500 0.00519698680016513 1.7314643242609469 >1k 0.0022901975729541185 2.5824133465106374 >5k 7.927606983302719E-4 2.9201949716563194 >10k+ 4.404226101834844E-4 4.235806946844992 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 35568 1.5475799918026296 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 22766 0.9905591006910331 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 14624 0.6362969466970776 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 13799 0.6004008183447055 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 10090 0.4390205273641625 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 9438 0.41065170835113635 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 8289 0.3606581914094691 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 8063 0.3508248277638496 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7523 0.3273291801150242 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 7309 0.318017941972712 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 7046 0.30657469135856186 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 7037 0.30618309723108145 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6572 0.28595073397792625 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5490 0.2388724177630577 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4222 0.18370115624692707 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4048 0.17613033644897225 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3911 0.17016940361954802 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3734 0.16246805244576637 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3712 0.1615108223563698 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3681 0.16016199813949278 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2972 0.12931308298575728 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2858 0.1243528907043386 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2780 0.12095907493284161 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2774 0.12069801218118799 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2349 0.10220606727239026 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2342 0.10190149406212771 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 2.1755229304467915E-5 14-15 0.0 0.0 0.0 0.0 4.351045860893583E-5 16-17 0.0 0.0 0.0 0.0 4.351045860893583E-5 18-19 0.0 0.0 0.0 4.351045860893583E-5 4.351045860893583E-5 20-21 0.0 0.0 0.0 4.351045860893583E-5 4.351045860893583E-5 22-23 0.0 0.0 0.0 1.0877614652233958E-4 8.702091721787166E-5 24-25 0.0 0.0 0.0 2.8281798095808287E-4 1.305313758268075E-4 26-27 0.0 0.0 0.0 4.133493567848904E-4 1.305313758268075E-4 28-29 0.0 0.0 0.0 7.396777963519091E-4 1.305313758268075E-4 30-31 0.0 0.0 0.0 0.0016969078857484972 1.522866051312754E-4 32-33 0.0 0.0 0.0 0.0035678576059327382 1.7404183443574332E-4 34-35 0.0 0.0 0.0 0.00563460438985719 1.7404183443574332E-4 36-37 0.0 0.0 0.0 0.008114700530566532 1.7404183443574332E-4 38-39 0.0 0.0 0.0 0.011508516302063527 1.7404183443574332E-4 40-41 0.0 0.0 0.0 0.01503286344938733 1.7404183443574332E-4 42-43 0.0 0.0 0.0 0.02158118747003217 1.7404183443574332E-4 44-45 2.1755229304467915E-5 0.0 0.0 0.02721579185988936 1.7404183443574332E-4 46-47 4.351045860893583E-5 0.0 0.0 0.03409044432010122 2.1755229304467916E-4 48-49 4.351045860893583E-5 0.0 0.0 0.040116642837438835 2.1755229304467916E-4 50-51 4.351045860893583E-5 0.0 0.0 0.049210328686706425 2.1755229304467916E-4 52-53 4.351045860893583E-5 0.0 0.0 0.07063922955160731 2.1755229304467916E-4 54-55 4.351045860893583E-5 0.0 0.0 0.10207553589656346 2.1755229304467916E-4 56-57 4.351045860893583E-5 0.0 0.0 0.12913904115132155 2.1755229304467916E-4 58-59 4.351045860893583E-5 0.0 0.0 0.1692556839887604 2.1755229304467916E-4 60-61 4.351045860893583E-5 0.0 0.0 0.2173782512102434 2.1755229304467916E-4 62-63 4.351045860893583E-5 0.0 0.0 0.24663903462475273 2.1755229304467916E-4 64-65 4.351045860893583E-5 0.0 0.0 0.2704610107131451 2.1755229304467916E-4 66-67 4.351045860893583E-5 0.0 0.0 0.34508144722747003 2.1755229304467916E-4 68-69 4.351045860893583E-5 0.0 0.0 0.4271639273932275 2.1755229304467916E-4 70-71 4.351045860893583E-5 0.0 0.0 0.45925289061731767 2.1755229304467916E-4 72-73 4.351045860893583E-5 0.0 0.0 0.5200152460646965 2.1755229304467916E-4 74-75 4.351045860893583E-5 0.0 0.0 0.6125619915259031 2.1755229304467916E-4 76-77 4.351045860893583E-5 0.0 0.0 0.7056961281783303 2.1755229304467916E-4 78-79 4.351045860893583E-5 0.0 0.0 0.7983081393274502 2.1755229304467916E-4 80-81 4.351045860893583E-5 0.0 0.0 0.9233136869109229 2.1755229304467916E-4 82-83 4.351045860893583E-5 0.0 0.0 1.0663108091291904 2.1755229304467916E-4 84-85 4.351045860893583E-5 0.0 0.0 1.2152471089475778 2.1755229304467916E-4 86-87 4.351045860893583E-5 0.0 0.0 1.4437205271030997 2.1755229304467916E-4 88-89 4.351045860893583E-5 0.0 0.0 1.7251679286150012 2.1755229304467916E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10125 0.0 25.721966 1 TACATGG 10380 0.0 24.632221 2 ACATGGG 10190 0.0 24.018297 3 GTATAGG 850 0.0 22.364527 1 GTGTAGC 1155 0.0 21.807833 1 GAGTACT 8190 0.0 21.583454 12-13 GTACTTT 8390 0.0 21.123516 14-15 CATGGGG 4590 0.0 20.501263 4 TACACCG 190 8.212521E-6 20.012545 5 AGTACTT 8545 0.0 19.824825 12-13 CATGGGA 5030 0.0 19.74724 4 TACCTGG 1670 0.0 19.63592 2 TATACCG 150 7.691761E-4 19.011917 5 GTATAGC 815 0.0 18.659973 1 GTAAGGT 765 0.0 18.016262 4 ACTTTTT 10285 0.0 17.577627 16-17 GTACCTG 1875 0.0 17.48906 1 TACTTTT 9665 0.0 17.402864 14-15 ATGGGAG 2210 0.0 16.77522 5 TAGCGCG 170 0.0017818507 16.77376 4 >>END_MODULE