##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936426_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2298298 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.43432096272981 31.0 31.0 34.0 30.0 34.0 2 31.630918618908428 31.0 31.0 34.0 30.0 34.0 3 31.916403355874653 31.0 31.0 34.0 30.0 34.0 4 35.70860784806844 37.0 35.0 37.0 33.0 37.0 5 35.52875780251299 37.0 35.0 37.0 33.0 37.0 6 35.650279902780234 37.0 35.0 37.0 33.0 37.0 7 35.58640567933314 37.0 35.0 37.0 33.0 37.0 8 35.56955668934142 37.0 35.0 37.0 33.0 37.0 9 37.18207212467661 39.0 37.0 39.0 33.0 39.0 10-11 37.055466697530086 39.0 37.0 39.0 33.0 39.0 12-13 36.94235103541838 39.0 37.0 39.0 33.0 39.0 14-15 37.98732061725677 40.0 37.0 41.0 33.0 41.0 16-17 37.93264863825318 40.0 37.0 41.0 33.0 41.0 18-19 38.030261741514806 40.0 37.0 41.0 33.0 41.0 20-21 38.12055769095217 40.0 37.0 41.0 33.0 41.0 22-23 38.04145415433508 40.0 37.0 41.0 33.0 41.0 24-25 37.90973842382493 40.0 37.0 41.0 33.0 41.0 26-27 37.681743620714116 39.5 37.0 41.0 32.0 41.0 28-29 37.62400545969235 39.0 37.0 41.0 32.0 41.0 30-31 37.64141878033223 39.0 37.0 41.0 32.0 41.0 32-33 37.46763800864814 39.0 36.0 41.0 32.0 41.0 34-35 37.26044860153035 39.0 36.0 41.0 31.0 41.0 36-37 37.0067530407284 39.0 35.5 41.0 31.0 41.0 38-39 36.84622229145219 39.0 35.0 41.0 31.0 41.0 40-41 36.67848251184137 39.0 35.0 40.5 30.5 41.0 42-43 36.4345905100209 39.0 35.0 40.0 30.0 41.0 44-45 36.1434152577255 39.0 35.0 40.0 30.0 41.0 46-47 35.74491928374823 38.0 35.0 40.0 28.5 41.0 48-49 35.26302159250019 38.0 34.0 40.0 27.0 41.0 50-51 35.04423686571541 38.0 34.0 40.0 26.5 41.0 52-53 34.80198607839367 38.0 34.0 40.0 26.0 41.0 54-55 34.55605147809379 38.0 34.0 40.0 25.5 41.0 56-57 34.22995103332988 38.0 33.0 40.0 24.5 41.0 58-59 33.856978947029496 37.0 33.0 40.0 23.0 41.0 60-61 33.39731792831043 36.5 32.0 39.5 22.0 41.0 62-63 33.15245455550151 36.0 32.0 39.0 22.0 40.0 64-65 32.73178586936942 36.0 31.5 39.0 21.5 40.0 66-67 32.267305632254825 35.0 31.0 38.5 20.0 40.0 68-69 31.781793309657843 35.0 31.0 38.0 19.0 40.0 70-71 31.24615324035438 34.5 30.0 37.0 18.0 39.0 72-73 30.80869887194785 34.0 30.0 37.0 15.5 39.0 74-75 30.45841466163222 34.0 29.5 36.0 12.5 39.0 76-77 29.049875168494253 32.5 28.0 35.0 11.0 37.0 78-79 29.85701114476887 34.0 29.0 35.0 9.0 37.0 80-81 29.650972589281288 34.0 29.0 35.0 7.0 37.0 82-83 29.288354251711482 34.0 29.0 35.0 4.5 36.5 84-85 28.82365406922862 33.5 29.0 35.0 2.0 36.0 86-87 28.511719324474022 33.0 29.0 35.0 2.0 36.0 88-89 28.05852026151526 33.0 27.0 35.0 2.0 35.0 90-91 27.54728303292262 33.0 26.0 35.0 2.0 35.0 92-93 27.097783664259378 33.0 25.5 34.5 2.0 35.0 94-95 26.511186756460653 32.0 25.0 34.0 2.0 35.0 96-97 25.756358183316525 32.0 23.5 34.0 2.0 35.0 98-99 24.895871423113974 32.0 19.0 34.0 2.0 35.0 100-101 22.00255797986162 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.45481487547059274 1101 2 0.4444341082552157 1101 3 0.2744821907915842 1101 4 0.19605306086373275 1101 5 0.20279218612206762 1101 6 0.18997986701584324 1101 7 0.28351185256732947 1101 8 0.1586126136269641 1101 9 0.28636522888206173 1101 10-11 0.29882286670397207 1101 12-13 0.275246277415377 1101 14-15 0.4568787805011212 1101 16-17 0.36928567243678145 1101 18-19 0.3314212814884101 1101 20-21 0.1922999736470743 1101 22-23 0.13817666421507369 1101 24-25 0.044719469237186615 1101 26-27 0.22768154282569242 1101 28-29 0.21076828266991754 1101 30-31 0.2250274584774843 1101 32-33 0.29919254528586237 1101 34-35 0.2896100472775416 1101 36-37 0.2912428902610671 1101 38-39 0.29705677977549527 1101 40-41 0.2786927624069264 1101 42-43 0.4136121564909132 1101 44-45 0.27258713677249347 1101 46-47 0.49154211224716704 1101 48-49 0.4911978017461962 1101 50-51 0.4915867245567256 1101 52-53 0.29058795424878525 1101 54-55 0.23319625756466422 1101 56-57 0.30161085497538664 1101 58-59 0.39900245638109055 1101 60-61 0.4998628243666161 1101 62-63 0.25657161575705345 1101 64-65 0.3119408372594705 1101 66-67 0.11529473392677403 1101 68-69 0.02484681945269429 1101 70-71 0.1451289088881964 1101 72-73 0.3754769277866252 1101 74-75 0.27032724279969855 1101 76-77 0.5063787057034901 1101 78-79 0.312023213942183 1101 80-81 0.24607010954077424 1101 82-83 0.3975226094486679 1101 84-85 0.27480651779558585 1101 86-87 0.4021211886980396 1101 88-89 0.18886161713769312 1101 90-91 0.12411829319559686 1101 92-93 0.23336336242495648 1101 94-95 0.2732741640856986 1101 96-97 0.41136678146570205 1101 98-99 0.4305551941228636 1101 100-101 0.4811139405972611 1102 1 0.4668955847674958 1102 2 0.5751956723773617 1102 3 0.24872329127274995 1102 4 0.18518039047003754 1102 5 0.2451090971294363 1102 6 0.17506705591059557 1102 7 0.23763391701998415 1102 8 0.012281054987752782 1102 9 0.22394177217417877 1102 10-11 0.3106270273283087 1102 12-13 0.30935319974731357 1102 14-15 0.4421664551735489 1102 16-17 0.49776775169848264 1102 18-19 0.3608737951829326 1102 20-21 0.392955331549409 1102 22-23 0.2490955808488664 1102 24-25 0.18983161957206107 1102 26-27 0.04486141251634024 1102 28-29 0.2291542056218887 1102 30-31 0.3077239985925715 1102 32-33 0.49473471519531387 1102 34-35 0.6089051059756798 1102 36-37 0.5392612516837616 1102 38-39 0.4356915741142444 1102 40-41 0.4918177912307655 1102 42-43 0.37749445148299543 1102 44-45 0.2692802423676497 1102 46-47 0.1736312381035532 1102 48-49 0.1678265725441932 1102 50-51 0.15768198540602185 1102 52-53 0.20571681600199554 1102 54-55 0.32604311077063386 1102 56-57 0.540492701596726 1102 58-59 0.4889935081820056 1102 60-61 0.46530384824043125 1102 62-63 0.35255674393984293 1102 64-65 0.3730854765387974 1102 66-67 0.4145018222839951 1102 68-69 0.38555356064285107 1102 70-71 0.36787165040630754 1102 72-73 0.36900949999993315 1102 74-75 -0.3193848583424952 1102 76-77 0.09565316496836473 1102 78-79 0.3570008850788895 1102 80-81 0.4162351355975211 1102 82-83 0.4429017325379654 1102 84-85 0.4938681354660055 1102 86-87 0.36093961647960526 1102 88-89 0.26073208539047954 1102 90-91 0.23248293775288786 1102 92-93 0.4351976151318375 1102 94-95 0.20593512277922343 1102 96-97 0.15635223503755213 1102 98-99 0.008847292292994524 1102 100-101 -0.16862843838106656 1103 1 0.4107058172552911 1103 2 0.49314002605037643 1103 3 0.2877075177059929 1103 4 0.0818375881206137 1103 5 0.05538233909079793 1103 6 0.17732511562800113 1103 7 0.16361536411208988 1103 8 0.2425640965439868 1103 9 0.07317181286016705 1103 10-11 0.14536028433926163 1103 12-13 0.1397873571309134 1103 14-15 0.1621822361783316 1103 16-17 0.18724835767418568 1103 18-19 0.2740917503217375 1103 20-21 0.24859005211529706 1103 22-23 0.1360529770946428 1103 24-25 0.08144885954497738 1103 26-27 0.1624022379633061 1103 28-29 0.21989921346899877 1103 30-31 0.1664128797404132 1103 32-33 0.12550569103910192 1103 34-35 0.14676402156155888 1103 36-37 0.23640766977997885 1103 38-39 0.24607615397753335 1103 40-41 0.28448244425940317 1103 42-43 0.3077883246597537 1103 44-45 0.2336992987057087 1103 46-47 0.28856511408196184 1103 48-49 0.3620678229501806 1103 50-51 0.018074096267262973 1103 52-53 0.3487774197710891 1103 54-55 0.3952422123814685 1103 56-57 0.30535983681749457 1103 58-59 0.3020517472797266 1103 60-61 0.44014326443003426 1103 62-63 0.20084827661879956 1103 64-65 0.13195330411551964 1103 66-67 0.22468301871473528 1103 68-69 0.17426761639826793 1103 70-71 0.1343340865322844 1103 72-73 0.10939864494963913 1103 74-75 0.2700560471871789 1103 76-77 0.2074801168914675 1103 78-79 0.3389517482614224 1103 80-81 0.31766676901209223 1103 82-83 0.42053164690153366 1103 84-85 0.4707667992252311 1103 86-87 0.5160357157325777 1103 88-89 0.5000603048766123 1103 90-91 0.04132784692223268 1103 92-93 0.43904253049953823 1103 94-95 0.4317252112421066 1103 96-97 0.1722957141230701 1103 98-99 0.0620878063347412 1103 100-101 0.17951967992316398 1104 1 0.2221649956451266 1104 2 0.25836877791935464 1104 3 0.0802286388132778 1104 4 0.005039459462238938 1104 5 -0.03551648574255495 1104 6 0.0705543865056697 1104 7 -0.0814252531687174 1104 8 -0.048791528697783804 1104 9 0.016412058103895788 1104 10-11 -0.1824485773703799 1104 12-13 -0.12245201683303719 1104 14-15 -0.009879301518310513 1104 16-17 -0.06641327594205393 1104 18-19 -0.12435474024577076 1104 20-21 -0.14009289056698293 1104 22-23 -0.11018299724110392 1104 24-25 -0.12163595290249418 1104 26-27 0.0423978482168792 1104 28-29 -0.1432904587430528 1104 30-31 -0.15395402787725487 1104 32-33 -0.28956916201959615 1104 34-35 -0.3367278186031655 1104 36-37 -0.18367302515810735 1104 38-39 -0.20190156138985316 1104 40-41 -0.22935980862636995 1104 42-43 -0.2548168716116379 1104 44-45 -0.20171443489488894 1104 46-47 -0.15612673360111273 1104 48-49 -0.12911880614898052 1104 50-51 -0.19744145944188318 1104 52-53 -0.3411981789381784 1104 54-55 -0.13126027534361384 1104 56-57 -0.07372106608786311 1104 58-59 -0.14570883025083958 1104 60-61 -0.3290012443245942 1104 62-63 -0.41408797201546577 1104 64-65 -0.28652223820612477 1104 66-67 -0.24826456261986962 1104 68-69 -0.24884990087271674 1104 70-71 -0.32366690843648627 1104 72-73 -0.2426662941941622 1104 74-75 -0.28301054538735926 1104 76-77 -0.1306313856993313 1104 78-79 -0.12398384615510949 1104 80-81 -0.15061284146460707 1104 82-83 -0.16214110235300083 1104 84-85 0.03882802891204307 1104 86-87 -0.02893478080594747 1104 88-89 -0.08929308971914907 1104 90-91 -0.22538471805995997 1104 92-93 -0.16063336191044542 1104 94-95 -0.12534141649890884 1104 96-97 -0.29326592179718247 1104 98-99 -0.24225334365755558 1104 100-101 -0.3866151240964868 1105 1 0.24801700795240578 1105 2 0.36642254236785377 1105 3 0.0915402457877228 1105 4 0.029474404731857362 1105 5 -0.1724324036283562 1105 6 -0.001042189706581098 1105 7 -0.07497186139863743 1105 8 -0.12348685027408379 1105 9 -0.0594092881278101 1105 10-11 -0.04688199684505889 1105 12-13 -0.10921491971198805 1105 14-15 0.08427891620901562 1105 16-17 -0.0058971097241169446 1105 18-19 -0.05484997483974752 1105 20-21 0.00362557984160361 1105 22-23 -0.09398344008206294 1105 24-25 -0.13643653931234923 1105 26-27 -0.05988447671016672 1105 28-29 -0.05387406930251615 1105 30-31 -0.032448188407705914 1105 32-33 -0.037088468732015656 1105 34-35 -0.19759807136852459 1105 36-37 -0.010058434954196116 1105 38-39 -0.10746701859370944 1105 40-41 -0.123297837323328 1105 42-43 0.05228637965315386 1105 44-45 -0.13906613543539947 1105 46-47 -0.006171501265789914 1105 48-49 0.03631168127642326 1105 50-51 0.06826874704502472 1105 52-53 0.02408636680142706 1105 54-55 -0.10980934046381918 1105 56-57 -0.1948275107093167 1105 58-59 0.03378329929035573 1105 60-61 0.10999068578198035 1105 62-63 -0.02639781831865662 1105 64-65 -0.04043982185152828 1105 66-67 -0.11056367129130251 1105 68-69 -0.3741015218796697 1105 70-71 -0.213711682108233 1105 72-73 -0.2128279924159493 1105 74-75 -0.32381196918231225 1105 76-77 -0.23351944821607518 1105 78-79 -0.11837700026160292 1105 80-81 -0.3360127709416467 1105 82-83 -0.04599867239840094 1105 84-85 -0.06866617727211377 1105 86-87 -0.175201599090979 1105 88-89 -0.14821257760469564 1105 90-91 -0.4033197408207343 1105 92-93 -0.28069345059877904 1105 94-95 0.05209944993636739 1105 96-97 0.03442249585734558 1105 98-99 -0.08652239176575094 1105 100-101 -0.019662641195601793 1106 1 0.268590485238974 1106 2 0.24877251916224452 1106 3 0.02265474268468992 1106 4 -0.04720338493540055 1106 5 -0.2793462869798873 1106 6 -0.15930791294001523 1106 7 -0.10611701746164215 1106 8 -0.14773957836651164 1106 9 -0.1564321201536032 1106 10-11 -0.15748441022838477 1106 12-13 -0.07073661016666932 1106 14-15 -0.09587816071712041 1106 16-17 -0.15224302598958417 1106 18-19 -0.10960842225474465 1106 20-21 -0.21259592539066574 1106 22-23 -0.1084078082044968 1106 24-25 -0.1203443246281779 1106 26-27 -0.2516144080831211 1106 28-29 -0.1323011062208579 1106 30-31 -0.20433392930033278 1106 32-33 -0.26528417379325475 1106 34-35 -0.534922829791789 1106 36-37 -0.2899905774082683 1106 38-39 -0.14175172154079263 1106 40-41 4.019394320735614E-4 1106 42-43 -0.2606826739020107 1106 44-45 -0.22183750979286998 1106 46-47 -0.1764554817911872 1106 48-49 -0.40212404944677616 1106 50-51 -0.4335187487227401 1106 52-53 -0.19979276305763705 1106 54-55 -0.36033644576176727 1106 56-57 -0.43299574322649903 1106 58-59 -0.44542052200051074 1106 60-61 -0.20994291720716518 1106 62-63 -0.7557369050738316 1106 64-65 -0.46522490592583665 1106 66-67 -0.5628295224724411 1106 68-69 -0.39394646676860745 1106 70-71 -0.3918416630910997 1106 72-73 -0.3578689326625657 1106 74-75 -0.3375633293810445 1106 76-77 -0.2796147197639449 1106 78-79 -0.30057950526031263 1106 80-81 -0.3861855456022063 1106 82-83 -0.3480313660535863 1106 84-85 -0.13206728063997275 1106 86-87 -0.14253042528549287 1106 88-89 -0.0848276940220174 1106 90-91 -0.13450637426928225 1106 92-93 0.3343555225907444 1106 94-95 0.21043714375148426 1106 96-97 -0.019531223890112415 1106 98-99 0.08101388170918256 1106 100-101 0.2022723022267705 1107 1 0.563824173694492 1107 2 0.550128303440566 1107 3 0.3362569757411471 1107 4 0.24222044436518786 1107 5 0.11096835729602361 1107 6 0.26792288638585404 1107 7 0.1918628489161165 1107 8 0.16736740971582975 1107 9 0.28558741156663103 1107 10-11 0.2892863854860792 1107 12-13 0.3332400811779834 1107 14-15 0.4077975796223541 1107 16-17 0.39633309956716545 1107 18-19 0.36905452766700364 1107 20-21 0.35345416790413964 1107 22-23 0.27123699437164817 1107 24-25 0.36069914774452627 1107 26-27 0.3771847221684723 1107 28-29 0.43424668479876516 1107 30-31 0.31214125728929076 1107 32-33 0.38772834576856496 1107 34-35 0.3340980261128621 1107 36-37 0.5709399630997396 1107 38-39 0.5348203712724953 1107 40-41 0.6030224703096465 1107 42-43 0.47096119572601225 1107 44-45 0.4276094927351153 1107 46-47 0.5656434257566687 1107 48-49 0.6495236573065597 1107 50-51 0.6752423112266897 1107 52-53 0.5258264436355091 1107 54-55 0.527188382696167 1107 56-57 0.5603588823904531 1107 58-59 0.39760907653085553 1107 60-61 0.3003571309449953 1107 62-63 0.09199053864832507 1107 64-65 0.1651267553237048 1107 66-67 0.2624853475167299 1107 68-69 0.291975045209238 1107 70-71 0.4027641663298738 1107 72-73 0.3309123662419715 1107 74-75 0.6241523170617178 1107 76-77 0.41411684698698537 1107 78-79 0.5109235945350434 1107 80-81 0.6277353193309843 1107 82-83 0.4902575017687347 1107 84-85 0.3532308865684044 1107 86-87 0.682001595901454 1107 88-89 0.810779549868677 1107 90-91 0.6661658750385335 1107 92-93 0.6182727008741189 1107 94-95 0.7182483270908371 1107 96-97 0.779769065022851 1107 98-99 0.8546321404066681 1107 100-101 0.8937444793063385 1108 1 0.4192499978703168 1108 2 0.4629373693746288 1108 3 0.3207006570598274 1108 4 0.13648555425529452 1108 5 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-1.5936272328647192 2316 90-91 -1.8922925012625846 2316 92-93 -2.229419929076574 2316 94-95 -2.275678310787413 2316 96-97 -2.4899728865739377 2316 98-99 -2.903873366183518 2316 100-101 -3.3899483399711556 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 17.0 4 36.0 5 74.0 6 175.0 7 410.0 8 997.0 9 1896.0 10 3071.0 11 4694.0 12 6811.0 13 9372.0 14 12221.0 15 15878.0 16 20135.0 17 25259.0 18 22023.0 19 15727.0 20 14741.0 21 15009.0 22 16439.0 23 18507.0 24 21725.0 25 25723.0 26 32003.0 27 39405.0 28 48700.0 29 60625.0 30 74103.0 31 91622.0 32 112130.0 33 141333.0 34 183068.0 35 244301.0 36 340642.0 37 420638.0 38 244012.0 39 14771.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.36660628712429 19.06867744175441 14.759082107822119 21.805634163299175 2 15.416261604646122 21.993801571974263 37.81202105133068 24.777915772048935 3 22.303276452264328 23.707818380314567 30.038852364929397 23.950052802491705 4 12.88273931732909 16.528864121178046 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23.444109510603063 25.85156494066479 29.111194457811823 21.593131090920327 26-27 22.676541510282828 25.00602619851734 30.88022092870463 21.437211362495205 28-29 21.050990776652984 28.04068488942687 29.635647770654632 21.272676563265513 30-31 21.762304488431113 25.861752014262727 29.275474563236482 23.100468934069678 32-33 21.906856286539274 26.2186865267434 29.757263472647434 22.117193714069895 34-35 23.6469664270672 25.55239135353395 29.15800076579007 21.642641453608785 36-37 22.873559217310714 24.9916457447688 30.389821732376653 21.744973305543834 38-39 21.89437716981879 26.36902811697909 30.376658134239747 21.35993657896237 40-41 22.162801671820308 26.726389867608034 29.362567925042647 21.74824053552901 42-43 21.464225601679324 27.08305411328124 28.057263753727046 23.39545653131239 44-45 22.51220442385482 25.62960555328208 28.315699221732064 23.542490801131038 46-47 23.690620994924195 26.99530143624153 27.909081475057928 21.404996093776344 48-49 23.394726959657834 26.584908098850036 28.03562733375485 21.984737607737284 50-51 21.383130733076737 27.15153814625334 29.558810348233422 21.9065207724365 52-53 22.89236781836255 28.0850177863708 27.07392172778531 21.948692667481346 54-55 21.676390751559786 26.55266975737074 28.075009769050084 23.695929722019397 56-57 23.09345767349959 26.420022700260613 27.635097988152964 22.851421638086833 58-59 24.911797575953507 25.936592589756312 26.98906065859695 22.162549175693226 60-61 22.7423499802451 26.76795476737082 29.35373628687944 21.135958965504635 62-63 22.08744105741379 26.98229187833493 28.854421983012003 22.075845081239276 64-65 21.606425248413302 28.526770454075173 27.728727690019017 22.13807660749251 66-67 22.37510524380666 26.97622305812939 26.910063592278423 23.738608105785527 68-69 21.874077440500965 27.037669470848698 27.725266594540688 23.362986494109652 70-71 23.410653743334123 26.908412059147047 27.12137640801437 22.55955778950446 72-73 23.023129077066827 25.78345492696224 28.965136149693414 22.228279846277516 74-75 21.82760895236388 28.870951460602583 27.23471890938425 22.066720677649286 76-77 22.444541473885916 26.775513684958458 27.194915831310617 23.58502900984501 78-79 22.688912357929265 27.020669146146126 27.65677007114252 22.633648424782088 80-81 23.59313910328617 26.911728324817517 27.166821984269223 22.328310587627087 82-83 23.01038664570394 26.48200841845271 28.291262102124108 22.216342833719242 84-85 22.310571908962427 27.501274950742516 27.90886756525311 22.279285575041946 86-87 22.35623523493773 27.922998228518885 27.129508715330253 22.591257821213127 88-89 21.91038864951908 28.032489010749877 26.139944292118205 23.91717804761283 90-91 22.56785927225092 26.57228530270654 26.90780626433455 23.952049160707993 92-93 23.512085218198347 27.376496334262523 27.21334985901177 21.89806858852736 94-95 22.827662911139697 26.8150021495794 27.571728070373926 22.78560686890699 96-97 21.230277315859063 26.945996522754722 29.01311027210282 22.810615889283397 98-99 22.105828159458053 27.87352420538153 26.623678681893264 23.396968953267155 100-101 21.312087121161024 26.76658075291085 27.41019416102672 24.51113796490141 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 393.0 1 303.0 2 282.0 3 364.5 4 315.0 5 273.5 6 507.5 7 1084.5 8 1696.0 9 2073.5 10 2127.5 11 2016.5 12 2038.0 13 2246.0 14 2520.5 15 2814.0 16 3102.0 17 3404.0 18 3749.5 19 4098.0 20 4528.0 21 5116.0 22 5868.0 23 6916.5 24 8328.5 25 10109.5 26 12578.0 27 15742.0 28 18544.5 29 21514.0 30 25546.0 31 30033.0 32 35403.5 33 39040.0 34 42056.5 35 45513.0 36 49793.0 37 54178.0 38 56122.0 39 57142.0 40 57654.0 41 59907.5 42 62886.5 43 65752.0 44 68229.0 45 71499.5 46 75714.5 47 105274.0 48 168531.5 49 162215.5 50 101781.5 51 78116.5 52 70948.0 53 68872.5 54 67952.0 55 63663.5 56 57548.0 57 51807.5 58 47245.0 59 41882.5 60 35268.5 61 29301.5 62 24720.0 63 19676.0 64 14785.0 65 11543.0 66 8601.5 67 6735.5 68 4980.0 69 3306.0 70 2506.5 71 2047.5 72 1764.0 73 1535.0 74 1330.0 75 1134.0 76 797.5 77 465.0 78 323.0 79 254.0 80 181.5 81 122.5 82 68.5 83 43.5 84 26.0 85 10.0 86 3.0 87 2.0 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0594361566689785 2 0.012879095748245006 3 0.004829660905591877 4 0.014097388589295209 5 0.0 6 0.0 7 0.0 8 0.0 9 0.005177744574463364 10-11 2.8281798095808287E-4 12-13 1.7404183443574332E-4 14-15 6.526568791340374E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0012835585289636068 24-25 0.0 26-27 0.0 28-29 0.0 30-31 2.1755229304467915E-5 32-33 0.0037418994403684816 34-35 7.831882549608448E-4 36-37 0.0029587111854076364 38-39 3.045732102625508E-4 40-41 0.0036766337524550777 42-43 0.01000740548005524 44-45 0.002327809535578067 46-47 0.0018491944908797728 48-49 0.010050915938664176 50-51 5.221255033072299E-4 52-53 0.0021537677011423235 54-55 0.009746342728401625 56-57 0.0037636546696729494 58-59 0.0014576003633993502 60-61 0.005830401453597401 62-63 0.003785409898977417 64-65 0.015816051704348175 66-67 0.002741158892362957 68-69 1.957970637402112E-4 70-71 0.0011312719238323315 72-73 2.1755229304467915E-5 74-75 0.0 76-77 0.0011530271531367995 78-79 0.01022495777309992 80-81 0.013858081066946061 82-83 0.010921125110842892 84-85 0.007396777963519091 86-87 0.009615811352574818 88-89 0.026019254248143626 90-91 0.022973522145518118 92-93 0.0024583409114048746 94-95 0.007570819797954834 96-97 0.009332993371616735 98-99 0.009311238142312267 100-101 0.011182187862496508 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2298298.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.16028324812246 #Duplication Level Percentage of deduplicated Percentage of total 1 75.30822272100912 35.515571144354816 2 13.882595047063667 13.094142292770089 3 4.683247841342148 6.625898841565612 4 2.029612041884251 3.828683151162459 5 1.1044282968867094 2.604257565420935 6 0.6550334308763374 1.853493728227051 7 0.41546412164514396 1.3715383959352176 8 0.3015327936820402 1.1376297566914149 9 0.21367084270178757 0.9069099717313188 >10 1.2103595915733714 10.740166744977838 >50 0.11426350013880184 3.784464248409937 >100 0.07165471096171012 6.206098164513661 >500 0.005745175089496595 1.8151068630618248 >1k 0.0028725875447482923 2.9502314752691374 >5k 7.413129147737529E-4 2.829355453041402 >10k+ 5.559846860803147E-4 4.736452202867282 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 40217 1.749860113875572 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 19247 0.8374457968461878 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 14146 0.6154989474820062 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 14000 0.6091464205251016 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 10702 0.46564892803283126 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 10072 0.43823733910920165 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 9776 0.4253582433609566 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8914 0.387852228040054 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8802 0.38297905667585314 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8430 0.366793166073329 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 8273 0.3599620240717261 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 7824 0.34042582815631395 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6616 0.2878651941567194 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 6109 0.26580539164198896 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4879 0.21228752755299793 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4727 0.20567393784443966 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3808 0.16568782638282764 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3625 0.15772541245739238 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3584 0.155941483654426 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3493 0.15198203192101284 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3288 0.143062387906181 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3241 0.14101739635156102 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2710 0.11791334283021611 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2573 0.11195241000079188 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2540 0.11051656486669702 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2392 0.1040770169925745 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2362 0.10277170323430643 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 4.351045860893583E-5 0.0 20-21 0.0 0.0 0.0 4.351045860893583E-5 0.0 22-23 0.0 0.0 0.0 1.0877614652233958E-4 0.0 24-25 0.0 0.0 0.0 2.8281798095808287E-4 0.0 26-27 0.0 0.0 0.0 4.133493567848904E-4 0.0 28-29 0.0 0.0 0.0 7.83188254960845E-4 0.0 30-31 0.0 0.0 0.0 0.0017404183443574333 0.0 32-33 0.0 0.0 0.0 0.0035025919180193344 0.0 34-35 0.0 0.0 0.0 0.00563460438985719 4.351045860893583E-5 36-37 0.0 0.0 0.0 0.008092945301262065 4.351045860893583E-5 38-39 0.0 0.0 0.0 0.011465005843454591 4.351045860893583E-5 40-41 0.0 0.0 0.0 0.015098129137300732 4.351045860893583E-5 42-43 0.0 0.0 0.0 0.021537677011423235 4.351045860893583E-5 44-45 0.0 0.0 0.0 0.027085260484062554 4.351045860893583E-5 46-47 0.0 0.0 0.0 0.03393815771496995 4.351045860893583E-5 48-49 0.0 0.0 0.0 0.03989909054439415 4.351045860893583E-5 50-51 0.0 0.0 0.0 0.04897102116435728 4.351045860893583E-5 52-53 0.0 0.0 0.0 0.07039992202925817 8.702091721787166E-5 54-55 0.0 0.0 0.0 0.10157516562256069 1.305313758268075E-4 56-57 0.0 0.0 0.0 0.12846462904288303 1.305313758268075E-4 58-59 0.0 0.0 0.0 0.16858127188032188 1.305313758268075E-4 60-61 0.0 0.0 0.0 0.21709543322928532 1.305313758268075E-4 62-63 0.0 0.0 0.0 0.24596462251631426 1.305313758268075E-4 64-65 0.0 0.0 0.0 0.2697430881460977 1.305313758268075E-4 66-67 0.0 0.0 0.0 0.34397193053294217 1.305313758268075E-4 68-69 0.0 0.0 0.0 0.4258803688642639 1.305313758268075E-4 70-71 0.0 0.0 0.0 0.45749071704365574 1.305313758268075E-4 72-73 0.0 0.0 0.0 0.5181225411152078 1.305313758268075E-4 74-75 0.0 0.0 0.0 0.6107780627229367 1.305313758268075E-4 76-77 0.0 0.0 0.0 0.7046736324010203 1.7404183443574332E-4 78-79 0.0 0.0 0.0 0.7970898464863999 1.7404183443574332E-4 80-81 0.0 0.0 0.0 0.9221824149870905 1.7404183443574332E-4 82-83 0.0 0.0 0.0 1.0648967192243999 1.7404183443574332E-4 84-85 0.0 0.0 0.0 1.2135066906032201 1.7404183443574332E-4 86-87 0.0 0.0 0.0 1.4403049561022985 1.7404183443574332E-4 88-89 0.0 0.0 0.0 1.7213172530281104 1.7404183443574332E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGGT 910 0.0 27.665798 4 TAAGGTG 995 0.0 27.206083 5 GTACATG 9770 0.0 24.570894 1 TACATGG 10170 0.0 23.216217 2 ACATGGG 9910 0.0 22.720268 3 GGTAAGG 970 0.0 22.526558 3 GAGTACT 10055 0.0 21.86678 12-13 GTACTTT 10580 0.0 20.647053 14-15 ATTCGCG 315 0.0 19.602152 80-81 AGTACTT 10455 0.0 19.41771 12-13 CATGGGG 4280 0.0 19.311419 4 TCGCGCT 340 0.0 18.85931 60-61 AGGTAAG 1040 0.0 18.728653 2 GTACCTG 1810 0.0 18.567993 1 CGCGGAA 320 0.0 18.554123 82-83 ACTTTTT 12115 0.0 18.051208 16-17 TACCTGG 1715 0.0 18.0055 2 ACCGGTC 160 0.0011929685 17.809244 8 CGATCGT 375 0.0 17.728935 24-25 TCGCGGA 335 0.0 17.723341 82-83 >>END_MODULE