##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936425_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2297313 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.280561682278382 31.0 31.0 34.0 28.0 34.0 2 31.281013079192952 31.0 31.0 34.0 28.0 34.0 3 31.345011324099065 31.0 31.0 34.0 28.0 34.0 4 34.95124652147966 37.0 35.0 37.0 32.0 37.0 5 34.93345356074684 37.0 35.0 37.0 32.0 37.0 6 35.082830245595616 37.0 35.0 37.0 32.0 37.0 7 34.94702027977903 37.0 35.0 37.0 32.0 37.0 8 34.896347167321125 37.0 35.0 37.0 32.0 37.0 9 36.332995982698044 39.0 37.0 39.0 32.0 39.0 10-11 36.23763305217878 39.0 35.0 39.0 32.0 39.0 12-13 36.1336755157003 39.0 35.0 39.0 31.0 39.0 14-15 37.112076586864745 39.0 36.0 41.0 31.0 41.0 16-17 37.01889185322157 39.0 36.0 41.0 31.0 41.0 18-19 36.99267557359402 39.0 36.0 41.0 31.0 41.0 20-21 36.890868810649664 39.0 36.0 41.0 30.5 41.0 22-23 36.747176592828225 39.0 36.0 40.5 30.0 41.0 24-25 36.873365536172045 39.0 36.0 41.0 30.0 41.0 26-27 36.848400283287475 39.0 36.0 41.0 30.0 41.0 28-29 36.773902163092274 39.0 36.0 41.0 30.0 41.0 30-31 36.61438907105823 39.0 36.0 41.0 30.0 41.0 32-33 36.428191326127525 39.0 35.0 40.5 30.0 41.0 34-35 36.1519529554745 39.0 35.0 40.0 30.0 41.0 36-37 35.93187671858384 38.0 35.0 40.0 28.5 41.0 38-39 35.713433694059106 38.0 35.0 40.0 27.5 41.0 40-41 35.59269807814607 38.0 35.0 40.0 27.0 41.0 42-43 35.339651366618305 38.0 34.0 40.0 26.5 41.0 44-45 35.05020256273307 38.0 34.0 40.0 25.5 41.0 46-47 34.726403846580766 38.0 34.0 40.0 24.0 41.0 48-49 34.57082339237187 38.0 33.5 40.0 24.0 41.0 50-51 33.614746880377204 37.5 32.0 39.5 22.5 40.5 52-53 33.78238011102536 37.5 33.0 39.5 23.0 40.5 54-55 34.21275986337082 38.0 33.0 40.0 23.0 41.0 56-57 34.05404748939304 38.0 33.0 40.0 22.5 41.0 58-59 33.77152307935401 38.0 33.0 40.0 22.0 41.0 60-61 33.35585116177029 37.0 32.5 40.0 20.5 41.0 62-63 33.06997305112538 36.5 32.0 40.0 20.0 41.0 64-65 32.73648497179096 36.0 32.0 39.0 18.5 41.0 66-67 32.25856097971848 35.5 31.0 39.0 17.5 40.5 68-69 31.826405457157993 35.0 31.0 38.5 13.5 40.0 70-71 31.31122641973471 35.0 30.5 37.5 10.0 40.0 72-73 30.901558255231222 35.0 30.0 37.0 8.0 39.0 74-75 30.496760780964543 34.5 30.0 36.5 7.0 39.0 76-77 30.06869394810372 34.0 29.0 36.0 4.5 39.0 78-79 29.58123882117935 34.0 29.0 36.0 2.0 37.5 80-81 29.02864455126489 34.0 28.5 35.0 2.0 37.0 82-83 28.51480425174976 34.0 27.5 35.0 2.0 36.5 84-85 28.177461669350237 34.0 27.0 35.0 2.0 36.0 86-87 27.935470917545846 34.0 27.0 35.0 2.0 36.0 88-89 27.467565151113497 33.0 26.0 35.0 2.0 35.5 90-91 26.989120333189252 33.0 25.0 35.0 2.0 35.0 92-93 26.593506631442906 33.0 25.0 35.0 2.0 35.0 94-95 26.090286782863284 33.0 24.0 35.0 2.0 35.0 96-97 25.435854626687785 32.0 20.5 35.0 2.0 35.0 98-99 24.731599264009738 32.0 10.0 35.0 2.0 35.0 100-101 22.208306834985045 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5894129507303596 1101 2 0.5077553311383625 1101 3 0.5544859752835727 1101 4 0.6443687663860942 1101 5 0.5478766005918843 1101 6 0.36213457686889683 1101 7 0.47161454422408866 1101 8 0.547019843779843 1101 9 0.7014389036799002 1101 10-11 0.6995188468077131 1101 12-13 0.7456070814515243 1101 14-15 0.9134247283825374 1101 16-17 1.002893402933978 1101 18-19 0.9641514485772333 1101 20-21 0.8578877451753186 1101 22-23 0.8661755629067329 1101 24-25 0.8733676568570701 1101 26-27 0.8637230485239158 1101 28-29 0.7403803326076712 1101 30-31 0.7932053981685812 1101 32-33 0.7614030730377834 1101 34-35 0.6731726719978894 1101 36-37 0.6128014620047182 1101 38-39 0.7759036217505297 1101 40-41 0.6520623764717257 1101 42-43 0.7027109042651887 1101 44-45 0.6961953820949915 1101 46-47 0.672356752854185 1101 48-49 0.7766386455054857 1101 50-51 0.6614274715622486 1101 52-53 0.7813788243481881 1101 54-55 0.904072594873405 1101 56-57 0.7419590579055466 1101 58-59 0.8094550951100601 1101 60-61 0.6857989283889623 1101 62-63 0.8330050951558761 1101 64-65 0.8798946102894831 1101 66-67 0.8839207960337987 1101 68-69 0.9707156128473748 1101 70-71 0.9552631774985727 1101 72-73 0.9850235392766535 1101 74-75 0.8190754584368207 1101 76-77 0.8479282121768641 1101 78-79 0.5822955601074185 1101 80-81 0.7792256675591283 1101 82-83 0.8348033293745196 1101 84-85 0.8246356231768921 1101 86-87 0.626812823494113 1101 88-89 0.6445821406991641 1101 90-91 0.6607890172491722 1101 92-93 0.7222631701412681 1101 94-95 0.6607791020145974 1101 96-97 0.6057559769584913 1101 98-99 0.7495575537998711 1101 100-101 1.2834363294834397 1102 1 0.3879672596972412 1102 2 0.49186360444113575 1102 3 0.450160336576797 1102 4 0.4107991489265004 1102 5 0.4853259954086653 1102 6 0.42536628803774335 1102 7 0.38633604480020267 1102 8 0.4497987210184462 1102 9 0.667433819773052 1102 10-11 0.4854651787265425 1102 12-13 0.49519972122098466 1102 14-15 0.5597808753280802 1102 16-17 0.5494260233369275 1102 18-19 0.6807157254558476 1102 20-21 0.6225142923166018 1102 22-23 0.5800153358820666 1102 24-25 0.3633380153932464 1102 26-27 0.4266281198769235 1102 28-29 0.5298751643421866 1102 30-31 0.44262580240234684 1102 32-33 0.3722317626483971 1102 34-35 0.17059377668815046 1102 36-37 0.41149914066310345 1102 38-39 0.32616425393650417 1102 40-41 0.26134838261431526 1102 42-43 0.46412158846565177 1102 44-45 0.11402303941184755 1102 46-47 0.3545958627946746 1102 48-49 0.49184702731504615 1102 50-51 0.580950137646802 1102 52-53 0.5432316917020401 1102 54-55 0.7640315110209244 1102 56-57 0.7846486829134349 1102 58-59 0.8347927495243397 1102 60-61 0.5844349572803935 1102 62-63 0.6998139321022379 1102 64-65 0.5838284411388344 1102 66-67 0.6869539485757983 1102 68-69 0.7278304037539165 1102 70-71 0.5970090253347919 1102 72-73 0.5203933938999441 1102 74-75 0.49887336554317585 1102 76-77 0.6098313748658839 1102 78-79 0.6953250104649626 1102 80-81 0.7641749684219832 1102 82-83 0.9958510715617486 1102 84-85 0.8430697031040957 1102 86-87 0.7505241554569153 1102 88-89 0.7526920835831774 1102 90-91 0.6141379749203288 1102 92-93 0.4325416495749579 1102 94-95 0.6599773352010452 1102 96-97 0.6718223779954222 1102 98-99 0.9541921609885087 1102 100-101 0.6710591192673299 1103 1 0.34647535430438836 1103 2 0.39548142932957475 1103 3 0.3654418583324812 1103 4 0.4217759844909921 1103 5 0.43780996610664147 1103 6 0.40181806306401313 1103 7 0.3085280613701613 1103 8 0.2759679843558942 1103 9 0.5775806344727954 1103 10-11 0.5684767837889098 1103 12-13 0.5060996465952456 1103 14-15 0.45748346895290837 1103 16-17 0.6364198248324939 1103 18-19 0.5996196410221657 1103 20-21 0.7322727486300877 1103 22-23 0.7467311184622858 1103 24-25 0.6760472339552521 1103 26-27 0.5345255018845094 1103 28-29 0.2563775782560782 1103 30-31 0.3134631335425979 1103 32-33 0.5072639735498541 1103 34-35 0.292582095477087 1103 36-37 0.3638357636204219 1103 38-39 0.4758170158499695 1103 40-41 0.21455728896663828 1103 42-43 0.2050043042330003 1103 44-45 0.2976937115003011 1103 46-47 0.4754937321539643 1103 48-49 0.3653796418046227 1103 50-51 0.5316255766440747 1103 52-53 0.5469184311668656 1103 54-55 0.5594823874925012 1103 56-57 0.7227569206801547 1103 58-59 0.48689331150176685 1103 60-61 0.4838786910036035 1103 62-63 0.5242907905113441 1103 64-65 0.534016909179222 1103 66-67 0.456181115387146 1103 68-69 0.516202877520687 1103 70-71 0.623785955323676 1103 72-73 0.7353318657477708 1103 74-75 0.5388738985840966 1103 76-77 0.6431376754095837 1103 78-79 0.7660370769884679 1103 80-81 0.6556333683854341 1103 82-83 0.4771200135516658 1103 84-85 0.6356733795634852 1103 86-87 0.7201046522510559 1103 88-89 0.927997820626473 1103 90-91 1.0022644883758005 1103 92-93 1.028999973389702 1103 94-95 0.9922868492200188 1103 96-97 1.0218791087792987 1103 98-99 1.1214977152749164 1103 100-101 1.606692143436927 1104 1 0.18753228647345566 1104 2 0.28252896234763725 1104 3 0.23507527963525376 1104 4 0.3474539100652976 1104 5 0.42220011352925724 1104 6 0.2136036065557505 1104 7 0.45328945164156664 1104 8 0.3094424230341062 1104 9 0.33819305528553656 1104 10-11 0.4599648547784767 1104 12-13 0.2044627655547302 1104 14-15 0.4778244833763523 1104 16-17 0.3092287067940376 1104 18-19 0.4479449744499675 1104 20-21 0.44325003598315504 1104 22-23 0.09131730785045278 1104 24-25 0.21501326830466638 1104 26-27 -0.1520532543811015 1104 28-29 0.25299533037118493 1104 30-31 0.1490134148676816 1104 32-33 -0.0167897645609969 1104 34-35 -0.10647488982787934 1104 36-37 -0.06401704402170338 1104 38-39 -0.06196615423805696 1104 40-41 -0.00785956810367594 1104 42-43 0.2045978145679399 1104 44-45 0.13482596676518455 1104 46-47 0.031358312350803885 1104 48-49 0.045550060456506 1104 50-51 0.0977127667584341 1104 52-53 0.2178585529071242 1104 54-55 0.22738505432447909 1104 56-57 0.2563259707873087 1104 58-59 0.24315936297274732 1104 60-61 0.26015306709953734 1104 62-63 0.40050671711294683 1104 64-65 0.05038143919215088 1104 66-67 0.326531723793245 1104 68-69 0.3279944726014854 1104 70-71 0.29501079694916044 1104 72-73 0.32320964726474344 1104 74-75 0.11297206421461325 1104 76-77 0.44117563215695554 1104 78-79 0.236652479315822 1104 80-81 0.39057553880998697 1104 82-83 0.34221799025452526 1104 84-85 0.46390330452378237 1104 86-87 0.5617028326415436 1104 88-89 0.3832969462456539 1104 90-91 0.49033946313625165 1104 92-93 0.3362666034516337 1104 94-95 0.23220062145592024 1104 96-97 0.39836307649628466 1104 98-99 0.4614113399110842 1104 100-101 0.9144340446395702 1105 1 0.22535605008809156 1105 2 0.2193310183238033 1105 3 0.1818919929334939 1105 4 0.20957320963932347 1105 5 0.3355613638864057 1105 6 0.26807777549502276 1105 7 0.12665688219325233 1105 8 0.3032762341825759 1105 9 0.3244969657623571 1105 10-11 0.3399141335444682 1105 12-13 0.27754172314226366 1105 14-15 0.18485429249502516 1105 16-17 0.20187193118079705 1105 18-19 0.18256672796811557 1105 20-21 0.12276559541901833 1105 22-23 0.1480183813349143 1105 24-25 0.32349521674073145 1105 26-27 0.2371558487859886 1105 28-29 0.10864621306867406 1105 30-31 0.027647559440012515 1105 32-33 0.0841841763066924 1105 34-35 -0.02481317232941649 1105 36-37 3.502276022686601E-4 1105 38-39 -0.20849138703472647 1105 40-41 -0.19057240518744578 1105 42-43 0.03478849244033455 1105 44-45 -0.1964954862056274 1105 46-47 0.00974023657913392 1105 48-49 -0.059216104983406126 1105 50-51 0.08083642755656939 1105 52-53 -0.058367247105195474 1105 54-55 -0.04710720996493478 1105 56-57 0.13515619500512344 1105 58-59 -0.007965514993479417 1105 60-61 -0.12896625222987979 1105 62-63 0.058269893591408106 1105 64-65 0.1905202989639463 1105 66-67 0.33710085896996844 1105 68-69 0.34431220595616807 1105 70-71 0.4156054549140187 1105 72-73 0.1525439141141014 1105 74-75 0.17028702916058336 1105 76-77 0.0027670499425305195 1105 78-79 0.19577422236656616 1105 80-81 0.029233112688526575 1105 82-83 0.16163954645463718 1105 84-85 0.3139990149793057 1105 86-87 0.3444091386514003 1105 88-89 0.2210287293408797 1105 90-91 0.39216378520930206 1105 92-93 0.3436336695605533 1105 94-95 0.322060458417905 1105 96-97 0.5873863610278995 1105 98-99 0.6749226133112742 1105 100-101 0.9837847615391873 1106 1 0.08894012881464874 1106 2 0.18689138101589364 1106 3 0.2984132274272575 1106 4 0.18359031081806165 1106 5 0.31291909910131466 1106 6 0.18863821499578393 1106 7 0.3509765241872671 1106 8 0.25597295553856725 1106 9 0.35911502107546056 1106 10-11 0.398880614706556 1106 12-13 0.5811878074130092 1106 14-15 0.7060604679120601 1106 16-17 0.7548116472369912 1106 18-19 0.6789853491826747 1106 20-21 0.44193781519732767 1106 22-23 0.4702949127174634 1106 24-25 0.30090470082697607 1106 26-27 0.14848598098224386 1106 28-29 0.5447756923136495 1106 30-31 0.5684716000880883 1106 32-33 0.17610630938430916 1106 34-35 0.2880688053309015 1106 36-37 0.41975651986158624 1106 38-39 0.45822601990459333 1106 40-41 0.33756998101740976 1106 42-43 0.14959115234783837 1106 44-45 0.31956536177759887 1106 46-47 0.4451675759656126 1106 48-49 0.4480942959190557 1106 50-51 0.12942272132102772 1106 52-53 0.44170762195154367 1106 54-55 0.5914501990846759 1106 56-57 0.376822027638255 1106 58-59 0.3101036857386177 1106 60-61 0.5420846377808815 1106 62-63 0.6357427642262152 1106 64-65 0.5459605639305849 1106 66-67 0.7239387236268797 1106 68-69 0.706043411301426 1106 70-71 0.8020851894508425 1106 72-73 0.8251793902541458 1106 74-75 0.5848445220014753 1106 76-77 0.7376697628402553 1106 78-79 0.7722576576847473 1106 80-81 0.9670305814161431 1106 82-83 1.1369481970504474 1106 84-85 1.116864443862628 1106 86-87 1.0661739225746487 1106 88-89 1.1845908516497587 1106 90-91 1.2002150406431298 1106 92-93 1.3480512542459877 1106 94-95 1.3876850098824285 1106 96-97 1.3892879574844592 1106 98-99 1.8569414710034309 1106 100-101 2.299267992810094 1107 1 0.131645017118867 1107 2 0.10306037544647495 1107 3 0.01548525491782371 1107 4 0.022446550905598883 1107 5 0.16658963034785756 1107 6 0.2187106297639687 1107 7 0.2639663074347496 1107 8 0.26280871294294883 1107 9 0.3642596661939166 1107 10-11 0.28523737731152465 1107 12-13 0.39953992576013775 1107 14-15 0.20331264096983404 1107 16-17 0.170131243484704 1107 18-19 0.3272608879903558 1107 20-21 0.4595228318564111 1107 22-23 0.5474340836974605 1107 24-25 0.4278401668510483 1107 26-27 0.24931808998552185 1107 28-29 0.43818697807535756 1107 30-31 0.38889442426405196 1107 32-33 0.21037961058794252 1107 34-35 0.3483981296236607 1107 36-37 0.4486737100702243 1107 38-39 0.38501679470572725 1107 40-41 0.18623418058218277 1107 42-43 0.42219648571395396 1107 44-45 0.5270853864896026 1107 46-47 0.48486798906174045 1107 48-49 0.5354380785763908 1107 50-51 0.4734872307078959 1107 52-53 0.36137633053434826 1107 54-55 0.5352638232655593 1107 56-57 0.5154288424949627 1107 58-59 0.7230040262970263 1107 60-61 0.5998171272998007 1107 62-63 0.5455081539012738 1107 64-65 0.6166410353027771 1107 66-67 0.43070048334263333 1107 68-69 0.5245335357220462 1107 70-71 0.6435397242873862 1107 72-73 0.6173543844142451 1107 74-75 0.5400016153292313 1107 76-77 0.41465287015919117 1107 78-79 0.33055769333887497 1107 80-81 0.4190188370685526 1107 82-83 0.6621520556879688 1107 84-85 0.5828517098055563 1107 86-87 0.5073231689201023 1107 88-89 0.49959386380904647 1107 90-91 0.270104051794263 1107 92-93 0.4656139102328112 1107 94-95 0.49983369375323505 1107 96-97 0.3740322927808215 1107 98-99 0.5970092599555663 1107 100-101 0.951572832550923 1108 1 -0.06324693370142143 1108 2 0.11001828655814094 1108 3 0.05419444362748038 1108 4 0.08151406055786481 1108 5 0.1824830743022261 1108 6 -4.36472056527748E-4 1108 7 -0.05838884606443173 1108 8 0.10866979524649878 1108 9 0.1279390234590423 1108 10-11 -0.08465754103465883 1108 12-13 0.030015401501387373 1108 14-15 0.10794448033610138 1108 16-17 0.07072412392152216 1108 18-19 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5469.0 3 2371.0 4 2229.0 5 2524.0 6 2867.0 7 3252.0 8 4215.0 9 5092.0 10 6606.0 11 8278.0 12 10332.0 13 12327.0 14 13993.0 15 16848.0 16 19882.0 17 24501.0 18 21735.0 19 17109.0 20 17779.0 21 19110.0 22 21094.0 23 23644.0 24 27021.0 25 30796.0 26 35967.0 27 42113.0 28 49619.0 29 58896.0 30 70854.0 31 85809.0 32 105598.0 33 134363.0 34 173296.0 35 229809.0 36 306444.0 37 374901.0 38 271969.0 39 38583.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.98021174702179 18.11232154474129 14.012934386639925 21.894532321596994 2 15.380382014742441 21.135202915398583 38.41420777875016 25.07020729110882 3 22.07165080550991 23.655110618833284 29.61281884495251 24.66041973070429 4 12.413066550878796 15.91400921454276 38.48702590811675 33.1858983264617 5 13.062850199149395 38.50928674246466 33.850649930422925 14.577213127963015 6 31.085876291802577 36.23240034665819 16.763013835842543 15.918709525696691 7 28.187741165608372 33.24292072783018 20.426411281542066 18.14292682501938 8 26.133301397900972 31.9169969080695 19.51617819971258 22.433523494316944 9 25.301198523206196 18.361504420539593 21.74638966766914 34.590907388585066 10-11 25.746938263555947 25.518248474004118 26.54247474057907 22.192338521860865 12-13 28.146553591885166 23.33525023641926 26.499642785363974 22.0185533863316 14-15 23.349137946050647 25.1768656352444 27.254247562245904 24.21974885645905 16-17 21.783877770773383 28.66302801068297 27.32543542474395 22.227658793799694 18-19 21.357028329076417 28.60840170926956 28.57732685240009 21.457243109253927 20-21 23.06332203189131 25.84238406444434 27.923791015614796 23.17050288804955 22-23 21.972163469953042 26.074586017821023 28.65319626373301 23.300054248492927 24-25 23.626160990712076 26.027283064075196 28.33304892615977 22.01350701905296 26-27 22.66676098951928 25.27653646485204 30.308218059924037 21.748484485704648 28-29 21.214696840552783 28.292153750651888 28.83566939204431 21.657480016751016 30-31 21.821098536090574 26.196545430602246 28.527329537972296 23.45502649533488 32-33 22.011897322852057 26.48363394413164 28.994190636196183 22.51027809682012 34-35 23.858459464977493 25.878343346522215 28.294241919397926 21.968955269102366 36-37 22.920482154292333 25.30643979579532 29.7779772557395 21.995100794172846 38-39 22.136030107985626 26.748089409746783 29.380448528883598 21.735431953383994 40-41 22.225339439984694 27.059389672945333 28.806676079261685 21.908594807808292 42-43 21.609357914458243 27.31636416188997 27.33758505770342 23.736692865948363 44-45 22.537663363164537 26.038944672760017 27.6742413458822 23.749150618193244 46-47 23.708609300350563 27.158070961983423 27.487239914847883 21.64607982281813 48-49 23.226997897522732 26.829677576622892 27.643375455427915 22.299949070426457 50-51 21.32617045222307 27.409577259281686 29.16543787256946 22.09881441592578 52-53 22.75787871757022 28.212462558216778 26.69378770024634 22.335871023966657 54-55 21.57099013995603 26.69492632174651 27.79252552075398 23.94155801754348 56-57 23.13602513257315 26.60226793087062 27.19681994236243 23.064886994193795 58-59 24.592830189336357 26.24423397397387 26.784573083345958 22.378362753343815 60-61 22.793382378229047 26.86938498850061 28.979173115073536 21.358059518196807 62-63 22.020505025045782 27.302930141263708 28.50393937296518 22.172625460725328 64-65 21.508528227772285 28.741829581702444 27.345436753910658 22.404205436614617 66-67 22.237850276277005 27.2674935169695 26.4837836084785 24.010872598274993 68-69 21.80088235607837 27.226717012379847 27.4974275317126 23.474973099829185 70-71 23.359169414607074 27.074580735033248 26.755514925941647 22.810734924418036 72-73 22.813377542656383 26.09917148873024 28.732729916992167 22.35472105162122 74-75 21.64059930659712 29.070248558965687 27.033570772343896 22.255581362093295 76-77 22.23725285254277 27.050063099626016 26.93213492654629 23.780549121284924 78-79 22.61211780346529 27.277203112335023 27.409408571023107 22.70127051317658 80-81 23.566484406289725 27.209130722482456 26.7318291047765 22.49255576645132 82-83 22.899734217548083 26.79544632885676 28.108208348542203 22.19661110505296 84-85 22.320967165431906 27.84499283611798 27.489454443500676 22.344585554949433 86-87 22.310210234590123 28.258422845531307 27.011502383642487 22.41986453623609 88-89 21.8671962454697 28.131573981307014 25.92266184740669 24.078567925816596 90-91 22.475135293257274 26.881525244294004 26.676408825926156 23.966930636522562 92-93 23.542457304986524 27.600742033609194 27.066420367002753 21.79038029440153 94-95 22.751836751219003 27.08910112650592 27.25557490155344 22.903487220721633 96-97 21.161497962207903 27.375349027945745 28.736699499454527 22.726453510391824 98-99 22.033759203735766 28.246573368329464 26.355201995101503 23.364465432833274 100-101 21.51104182678386 26.94299356192807 27.286342883394042 24.25962172789403 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 306.0 1 222.0 2 190.5 3 264.0 4 274.5 5 287.0 6 477.5 7 876.5 8 1419.5 9 1800.0 10 1803.0 11 1779.0 12 1834.5 13 2035.0 14 2273.5 15 2461.0 16 2635.5 17 2805.0 18 3080.0 19 3438.0 20 3832.5 21 4332.0 22 5153.0 23 6201.5 24 7707.0 25 9670.5 26 12069.0 27 15169.5 28 17921.5 29 20980.5 30 24941.5 31 29235.5 32 34767.5 33 38654.5 34 41858.5 35 45415.0 36 49741.5 37 54351.5 38 56410.0 39 57082.5 40 57675.0 41 60311.0 42 63248.5 43 66245.5 44 69533.0 45 73773.5 46 78678.5 47 105456.5 48 162715.0 49 158623.5 50 104397.5 51 80840.5 52 72129.5 53 69807.0 54 69044.5 55 64465.5 56 58368.5 57 52285.5 58 47354.5 59 41970.0 60 35410.0 61 29578.5 62 25030.0 63 20107.0 64 15200.0 65 12002.0 66 8957.5 67 6869.0 68 5165.0 69 3534.0 70 2673.5 71 2140.0 72 1814.0 73 1625.5 74 1365.5 75 1139.0 76 849.5 77 491.5 78 272.0 79 178.5 80 129.0 81 92.0 82 56.0 83 24.0 84 19.0 85 15.5 86 10.5 87 7.0 88 5.0 89 5.0 90 4.5 91 3.5 92 2.0 93 0.5 94 2.0 95 2.0 96 1.0 97 1.5 98 2.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03477976227009554 2 0.023636309027111238 3 0.02206926091481657 4 0.050493772507272626 5 0.05650079027106885 6 0.0480126129961394 7 0.051059650992267926 8 0.04409499271540273 9 0.042963235745412144 10-11 0.051777881377069644 12-13 0.04677203324057279 14-15 0.040786780033891766 16-17 0.049427309208627646 18-19 0.05417198265974206 20-21 0.011361098814136342 22-23 0.020676329259443533 24-25 0.005963488649565819 26-27 0.01035992918683697 28-29 0.006050546878026634 30-31 0.009162878545500765 32-33 0.003199389895934947 34-35 0.0076611241045517085 36-37 0.008966997531463933 38-39 0.010795220329141044 40-41 0.0015888126694098715 42-43 0.0021329265972899643 44-45 0.0023505721684420012 46-47 5.44113927880093E-4 48-49 0.001001169627299371 50-51 2.8293924249764833E-4 52-53 0.003482329138432595 54-55 0.007095245619556412 56-57 0.01625812416505718 58-59 0.010316400072606563 60-61 0.010185812729915339 62-63 0.005223493707648893 64-65 4.352911423040744E-5 66-67 0.0070517165053260045 68-69 0.017172235563895737 70-71 0.006007017763796226 72-73 0.0026117468538244463 74-75 0.0039829139520822805 76-77 0.006616425363021931 78-79 0.005702313964183374 80-81 0.010142283615684934 82-83 0.005811136749759392 84-85 0.01627988872217238 86-87 0.004069972180543095 88-89 0.0061593696636026525 90-91 0.0035258582526630023 92-93 0.016628121636015642 94-95 0.011078159571638692 96-97 0.009532876016459229 98-99 0.021220443187323624 100-101 0.03560681544047328 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2297313.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.40212329076655 #Duplication Level Percentage of deduplicated Percentage of total 1 77.10903744552465 38.09350174716143 2 13.041679717867046 12.885733386815149 3 4.236460866305325 6.278704861011701 4 1.8921381352657487 3.7390256576623857 5 0.9666848144667722 2.387814119379964 6 0.5887428619123934 1.7451088470452885 7 0.38577048527260144 1.334051675276313 8 0.2765057461186837 1.0927976768288488 9 0.2090267136665111 0.9293727131655065 >10 1.11177139491793 10.414201856085588 >50 0.1068725298361859 3.6504762947854146 >100 0.06635363588959156 5.895832023422293 >500 0.005497529537783397 1.8204828253431844 >1k 0.0022167457813642654 2.534106485865948 >5k 7.980284812911355E-4 2.9372418702985206 >10k+ 4.433491562728531E-4 4.261547959852528 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 35540 1.5470247197486802 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 22968 0.999776695643998 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 14740 0.6416191437562055 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 14020 0.6102781815103123 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 10017 0.4360311372459913 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 9570 0.4165736231849992 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 8569 0.37300097984036135 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 8143 0.35445757717820775 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7488 0.32594600735729085 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 7157 0.31153787054702603 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 7060 0.30731554646667647 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 6863 0.29874031096328624 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6565 0.28576863492262483 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5638 0.24541714603103712 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4377 0.19052693298649337 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4114 0.17907877594389618 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3887 0.1691976670135937 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3773 0.16423534799132725 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3594 0.15644363654408433 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3547 0.15439776817525516 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2933 0.12767089203778503 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2919 0.1270614844385593 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2797 0.12175093250244962 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2767 0.12044505907553738 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2397 0.10433928681028662 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2328 0.10133577792838852 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.352911423040744E-5 0.0 10-11 0.0 0.0 0.0 4.352911423040744E-5 0.0 12-13 0.0 0.0 0.0 4.352911423040744E-5 0.0 14-15 0.0 0.0 0.0 4.352911423040744E-5 2.176455711520372E-5 16-17 0.0 0.0 0.0 4.352911423040744E-5 4.352911423040744E-5 18-19 0.0 0.0 0.0 4.352911423040744E-5 4.352911423040744E-5 20-21 0.0 0.0 0.0 6.529367134561116E-5 4.352911423040744E-5 22-23 0.0 0.0 0.0 2.394101282672409E-4 4.352911423040744E-5 24-25 0.0 0.0 0.0 3.917620280736669E-4 4.352911423040744E-5 26-27 0.0 0.0 0.0 4.352911423040743E-4 4.352911423040744E-5 28-29 0.0 0.0 0.0 8.923468417233525E-4 4.352911423040744E-5 30-31 0.0 0.0 0.0 0.001937045583253131 4.352911423040744E-5 32-33 0.0 0.0 0.0 0.0042658531945799286 8.705822846081487E-5 34-35 0.0 0.0 0.0 0.006899364605519579 1.7411645692162975E-4 36-37 0.0 0.0 0.0 0.009489346902228822 1.7411645692162975E-4 38-39 0.0 0.0 0.0 0.012732265912394175 1.7411645692162975E-4 40-41 0.0 0.0 0.0 0.016932825435628494 1.7411645692162975E-4 42-43 0.0 0.0 0.0 0.022678668514042273 1.7411645692162975E-4 44-45 0.0 0.0 0.0 0.02827215969264963 1.7411645692162975E-4 46-47 0.0 0.0 0.0 0.03477976227009554 1.9588101403683345E-4 48-49 0.0 0.0 0.0 0.04067795724831575 2.611746853824446E-4 50-51 0.0 0.0 0.0 0.05060259529284865 2.611746853824446E-4 52-53 0.0 0.0 0.0 0.07343361570669735 2.611746853824446E-4 54-55 0.0 0.0 0.0 0.10749514759199116 2.611746853824446E-4 56-57 0.0 0.0 0.0 0.13966316300826226 2.611746853824446E-4 58-59 0.0 0.0 0.0 0.18116817342695574 2.611746853824446E-4 60-61 0.0 0.0 0.0 0.2287019661665607 2.611746853824446E-4 62-63 0.0 0.0 0.0 0.25847588030015933 2.611746853824446E-4 64-65 0.0 0.0 0.0 0.28409276402475414 2.611746853824446E-4 66-67 0.0 0.0 0.0 0.35974636455720227 2.611746853824446E-4 68-69 0.0 0.0 0.0 0.4431699119797781 2.611746853824446E-4 70-71 0.0 0.0 0.0 0.47496792992509074 3.0470379961285203E-4 72-73 0.0 0.0 0.0 0.537279856945919 3.482329138432595E-4 74-75 0.0 0.0 0.0 0.6309327462126406 3.482329138432595E-4 76-77 0.0 0.0 0.0 0.7215603620403489 3.482329138432595E-4 78-79 0.0 0.0 0.0 0.8149085475074576 3.482329138432595E-4 80-81 0.0 0.0 0.0 0.9409253332044871 3.482329138432595E-4 82-83 0.0 0.0 0.0 1.0894902000728677 3.482329138432595E-4 84-85 0.0 0.0 0.0 1.2458903075027217 3.482329138432595E-4 86-87 0.0 0.0 0.0 1.4673229115928041 3.482329138432595E-4 88-89 0.0 0.0 0.0 1.7473892325512457 3.482329138432595E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10320 0.0 24.26212 1 TACATGG 10580 0.0 23.441862 2 ACATGGG 10315 0.0 23.261057 3 GTACCTG 1830 0.0 22.587385 1 TACCTGG 1780 0.0 22.154676 2 GAGTACT 7915 0.0 21.762646 12-13 GTACTTT 8195 0.0 21.135044 14-15 GTATAGG 685 0.0 20.114313 1 AGTACTT 8095 0.0 19.840584 12-13 TACTGCG 145 6.135971E-4 19.661175 7 GTACACG 295 3.0122465E-9 19.326672 1 GTAAGCG 310 0.0 19.15386 94-95 GTGTAGC 945 0.0 19.105112 1 CATGGGA 5170 0.0 19.024183 4 CATGGGG 4545 0.0 18.399456 4 GTAAGGT 880 0.0 18.357876 4 ACTTTTT 9740 0.0 18.24439 16-17 TAAGGTG 1010 0.0 17.875965 5 TACTTTT 9350 0.0 17.152079 14-15 CTCGCGC 290 0.0 16.377892 60-61 >>END_MODULE