##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936425_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2297313 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.80198823582159 31.0 31.0 34.0 30.0 34.0 2 31.924882242863728 31.0 31.0 34.0 30.0 34.0 3 32.03845144305543 33.0 31.0 34.0 30.0 34.0 4 35.775595227990266 37.0 35.0 37.0 35.0 37.0 5 35.558909473807006 37.0 35.0 37.0 33.0 37.0 6 35.67681243261149 37.0 35.0 37.0 33.0 37.0 7 35.62617675519183 37.0 35.0 37.0 33.0 37.0 8 35.608467370358326 37.0 35.0 37.0 33.0 37.0 9 37.216421532459876 39.0 37.0 39.0 33.0 39.0 10-11 37.07291844863978 39.0 37.0 39.0 33.0 39.0 12-13 36.9877075957869 39.0 37.0 39.0 33.0 39.0 14-15 37.99995712382248 40.0 37.0 41.0 33.0 41.0 16-17 37.95594353055069 40.0 37.0 41.0 33.0 41.0 18-19 38.15670938178646 40.0 37.5 41.0 33.0 41.0 20-21 38.19909933909746 40.0 37.5 41.0 33.0 41.0 22-23 38.110466227283794 40.0 37.0 41.0 33.0 41.0 24-25 37.96032103592327 40.0 37.0 41.0 33.0 41.0 26-27 37.72055418656491 39.5 37.0 41.0 32.5 41.0 28-29 37.65814845430292 39.5 37.0 41.0 32.5 41.0 30-31 37.65886320235858 39.5 37.0 41.0 33.0 41.0 32-33 37.50199058639376 39.0 36.0 41.0 32.0 41.0 34-35 37.28613536771002 39.0 36.0 41.0 31.5 41.0 36-37 37.00776537633314 39.0 35.5 41.0 31.0 41.0 38-39 36.83409966338936 39.0 35.0 41.0 31.0 41.0 40-41 36.67805105355691 39.0 35.0 40.5 30.5 41.0 42-43 36.41725224207585 39.0 35.0 40.0 30.0 41.0 44-45 36.15860093944535 39.0 35.0 40.0 30.0 41.0 46-47 35.78633538398991 38.0 35.0 40.0 28.5 41.0 48-49 35.38462042394745 38.0 35.0 40.0 27.0 41.0 50-51 35.04055106988034 38.0 34.0 40.0 26.5 41.0 52-53 34.795635379245226 38.0 34.0 40.0 26.0 41.0 54-55 34.548829001533534 38.0 34.0 40.0 25.5 41.0 56-57 34.176752797724994 38.0 33.0 40.0 24.5 41.0 58-59 33.83436823802416 37.0 33.0 40.0 23.0 41.0 60-61 33.38164847367337 36.5 32.0 39.0 22.0 40.5 62-63 33.026231514817525 36.0 32.0 39.0 22.0 40.0 64-65 32.62293122443481 36.0 31.0 39.0 21.5 40.0 66-67 32.099053764114856 35.0 31.0 38.0 19.5 40.0 68-69 31.78429778615278 35.0 31.0 38.0 18.5 40.0 70-71 31.290843259059606 35.0 30.0 37.0 17.5 39.0 72-73 30.807028689603897 34.0 30.0 37.0 15.0 39.0 74-75 30.298581429696345 34.0 29.0 36.0 11.0 39.0 76-77 28.905705491589522 32.5 28.0 34.5 9.0 36.5 78-79 29.696697837865365 34.0 29.0 35.0 7.5 37.0 80-81 29.442066231288464 34.0 29.0 35.0 7.0 37.0 82-83 29.07537588478366 34.0 29.0 35.0 2.0 36.0 84-85 28.63717438590214 33.0 28.5 35.0 2.0 36.0 86-87 28.319387475716198 33.0 28.0 35.0 2.0 35.5 88-89 27.876757542398444 33.0 27.0 35.0 2.0 35.0 90-91 27.330516346705913 33.0 26.0 35.0 2.0 35.0 92-93 26.839051753069782 32.0 25.0 34.0 2.0 35.0 94-95 26.183975801294817 32.0 24.5 34.0 2.0 35.0 96-97 25.331098330963172 32.0 21.5 34.0 2.0 35.0 98-99 24.359276467769085 31.0 10.0 34.0 2.0 35.0 100-101 21.0356111683519 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3041893193821359 1101 2 0.4099898181003212 1101 3 0.33907561855359347 1101 4 0.22537749366676252 1101 5 0.3110153596891365 1101 6 0.26759953879327725 1101 7 0.22126251169005684 1101 8 0.24406256369952217 1101 9 0.3209979042655462 1101 10-11 0.2967575342060087 1101 12-13 0.4103738400206538 1101 14-15 0.5128503197615046 1101 16-17 0.5741344441711789 1101 18-19 0.5016708684357596 1101 20-21 0.4110315166023 1101 22-23 0.4633693587497234 1101 24-25 0.2764998368088172 1101 26-27 0.4313908514049274 1101 28-29 0.4215825071220607 1101 30-31 0.5245663527740874 1101 32-33 0.4586097324926115 1101 34-35 0.425908782634572 1101 36-37 0.45028171134534745 1101 38-39 0.3947542096580676 1101 40-41 0.475625958546523 1101 42-43 0.4614530220065092 1101 44-45 0.5272814661476986 1101 46-47 0.5070721401382983 1101 48-49 0.4998595974444626 1101 50-51 0.6671321901699443 1101 52-53 0.5768099441213224 1101 54-55 0.5514151452126228 1101 56-57 0.6410599883569859 1101 58-59 0.6815877270180053 1101 60-61 0.5264643715776032 1101 62-63 0.5445985979804462 1101 64-65 0.5614179866108984 1101 66-67 0.4678173812948643 1101 68-69 0.3323228594724448 1101 70-71 0.4721074664024272 1101 72-73 0.3844728572127103 1101 74-75 0.21430544209111346 1101 76-77 0.28380139999886467 1101 78-79 0.0967896580551475 1101 80-81 0.2817110013763262 1101 82-83 0.400207871788286 1101 84-85 0.5739892887260147 1101 86-87 0.497785483062696 1101 88-89 0.296373695132214 1101 90-91 0.2584738364418264 1101 92-93 0.27386220781814785 1101 94-95 0.41387113411863297 1101 96-97 0.342389929059852 1101 98-99 0.8505789376181028 1101 100-101 1.0421517487949465 1102 1 0.29512784313008567 1102 2 0.2867600099982184 1102 3 0.3079552000068446 1102 4 0.17627565320932348 1102 5 0.13066108542523125 1102 6 0.2346696076315169 1102 7 0.2113283434061799 1102 8 0.2736170301891576 1102 9 0.2932585476460332 1102 10-11 0.32736188583197645 1102 12-13 0.33050708667649076 1102 14-15 0.4335347361237254 1102 16-17 0.3692763465822395 1102 18-19 0.3410892176772933 1102 20-21 0.22599433883426912 1102 22-23 0.24673359692732078 1102 24-25 0.2956388510362373 1102 26-27 0.1386243441001085 1102 28-29 0.10140928007260896 1102 30-31 0.27258296542724736 1102 32-33 0.31525795220566266 1102 34-35 0.27529784223973763 1102 36-37 0.45218637729715994 1102 38-39 0.5030666132553421 1102 40-41 0.5194920466169393 1102 42-43 0.5331453041113718 1102 44-45 0.6287848888691911 1102 46-47 0.6077239005150048 1102 48-49 0.5447689339058641 1102 50-51 0.41051699426201793 1102 52-53 0.3153719133316244 1102 54-55 0.4105136033287664 1102 56-57 0.5020390442279847 1102 58-59 0.38592991760774 1102 60-61 0.4534592133072195 1102 62-63 0.4497990076995251 1102 64-65 0.5685946271863642 1102 66-67 0.48229548123465094 1102 68-69 0.3499041576038415 1102 70-71 0.3571380873858949 1102 72-73 0.29527563952826696 1102 74-75 0.38680220967629 1102 76-77 0.20102527341133936 1102 78-79 0.28581120189005205 1102 80-81 0.5700232130677172 1102 82-83 0.5505926031160477 1102 84-85 0.48086279795709075 1102 86-87 0.40454336899543364 1102 88-89 0.3901426708265383 1102 90-91 0.5602088256031372 1102 92-93 0.6441413429302862 1102 94-95 0.8051155878385288 1102 96-97 0.8322026049236051 1102 98-99 1.0662229457964933 1102 100-101 1.6349577567286566 1103 1 0.21207092958932705 1103 2 0.18619117918540695 1103 3 0.1253521327370848 1103 4 0.14981550182569947 1103 5 -0.019306170632397368 1103 6 0.1708006443199679 1103 7 0.16008940400531912 1103 8 0.2457961014795771 1103 9 0.2182345608045324 1103 10-11 0.17048565793413673 1103 12-13 0.28526333467758747 1103 14-15 0.25645473429614896 1103 16-17 0.30967551808434024 1103 18-19 0.1813520830006894 1103 20-21 0.24953304522009745 1103 22-23 0.13462519163346798 1103 24-25 0.09624385248306311 1103 26-27 0.21073297784007394 1103 28-29 0.27058961543149707 1103 30-31 0.1813575804955576 1103 32-33 0.2562571754888907 1103 34-35 0.25227199736988126 1103 36-37 0.2731421196708723 1103 38-39 0.3650505154194903 1103 40-41 0.43672145220016034 1103 42-43 0.28816502662549226 1103 44-45 0.29103949967317533 1103 46-47 0.21516553253400872 1103 48-49 0.3510188387199804 1103 50-51 0.3306670833560048 1103 52-53 0.237767246939093 1103 54-55 0.18695243307549703 1103 56-57 0.08642018371718052 1103 58-59 0.1781665573177662 1103 60-61 0.1681170771838012 1103 62-63 0.0934904683839477 1103 64-65 0.19874418323941967 1103 66-67 0.5237839599358622 1103 68-69 0.381920118372026 1103 70-71 0.28075598842304217 1103 72-73 0.16878464791985337 1103 74-75 0.19632193980528356 1103 76-77 -0.02290369507832679 1103 78-79 -0.173584025109232 1103 80-81 0.060158910056795634 1103 82-83 0.2869555126056973 1103 84-85 0.09977394765020975 1103 86-87 0.08016283869586616 1103 88-89 0.04192721568573532 1103 90-91 0.18176538124499686 1103 92-93 0.3094498974756057 1103 94-95 0.4650788841635922 1103 96-97 0.5967812741488672 1103 98-99 0.729367354197219 1103 100-101 0.8865207088615819 1104 1 0.1140133420774454 1104 2 0.1914507480761074 1104 3 0.11487078048736521 1104 4 0.1153503737386572 1104 5 0.020856307153842124 1104 6 0.12669075927063744 1104 7 0.16769964624599965 1104 8 0.035525226449315994 1104 9 0.07481101451430305 1104 10-11 0.1546821545068724 1104 12-13 0.0740383749723108 1104 14-15 0.14453812224139284 1104 16-17 0.2949875734401459 1104 18-19 0.17215366763143436 1104 20-21 0.06769947277519606 1104 22-23 0.13122478898139178 1104 24-25 0.15028941356869296 1104 26-27 0.17048444940941465 1104 28-29 0.17781344157081946 1104 30-31 -0.00999543673819403 1104 32-33 0.129625460439037 1104 34-35 0.09895749776249829 1104 36-37 0.20065042953584822 1104 38-39 0.06172612662998489 1104 40-41 0.09401169821939703 1104 42-43 -0.10894998899055963 1104 44-45 0.02747892179070277 1104 46-47 0.11022256507089878 1104 48-49 0.05863690374651753 1104 50-51 -0.04888932040601901 1104 52-53 0.09598530488084833 1104 54-55 0.15631043327622507 1104 56-57 0.14316047313073454 1104 58-59 -0.0708200229144964 1104 60-61 0.0859660177129129 1104 62-63 -0.11861964692096905 1104 64-65 -0.05906118584847064 1104 66-67 -0.17860907453753327 1104 68-69 0.11398527479822818 1104 70-71 0.027523997066484895 1104 72-73 0.03419863565136083 1104 74-75 0.15599961150805441 1104 76-77 0.009542563858836672 1104 78-79 0.02593834215432267 1104 80-81 0.08250344325788106 1104 82-83 0.023605559542410504 1104 84-85 0.15897782420960027 1104 86-87 0.15251917987560404 1104 88-89 0.22870685518814682 1104 90-91 0.2516938544143166 1104 92-93 0.3074226151409327 1104 94-95 0.3582178581683273 1104 96-97 0.6008039572758541 1104 98-99 0.8800290438008815 1104 100-101 1.214286727860621 1105 1 0.10907729500181773 1105 2 0.13805398518888268 1105 3 0.11974210653715289 1105 4 0.07131294935831534 1105 5 -0.012569817554577867 1105 6 0.04477177938015586 1105 7 0.08252500709889432 1105 8 0.10504710761090053 1105 9 -0.10049568795975006 1105 10-11 -0.004407876715490033 1105 12-13 -0.04277644239792977 1105 14-15 0.061819417511095764 1105 16-17 0.19225886717365626 1105 18-19 0.06385755257366554 1105 20-21 0.08389749434632421 1105 22-23 0.14615721226928002 1105 24-25 0.11263253879517521 1105 26-27 0.12370847055181144 1105 28-29 0.11891318006249207 1105 30-31 0.16585505503840636 1105 32-33 0.11948408848892456 1105 34-35 0.25628391788775673 1105 36-37 0.02638582403482559 1105 38-39 0.23860006569904613 1105 40-41 0.3841253182653972 1105 42-43 0.19655035466482218 1105 44-45 0.23069232809514517 1105 46-47 0.33788901973566254 1105 48-49 0.24659118563946691 1105 50-51 0.267720851734218 1105 52-53 0.17695189792181765 1105 54-55 0.32722244541016465 1105 56-57 0.16413988948566782 1105 58-59 0.22674491851666545 1105 60-61 0.17367443342181588 1105 62-63 0.3295236999787292 1105 64-65 0.15601083730130227 1105 66-67 0.28576907729534184 1105 68-69 0.39627155756749133 1105 70-71 0.314181381647078 1105 72-73 0.16225559841008774 1105 74-75 -0.08451227136074735 1105 76-77 -0.0703534785462594 1105 78-79 0.18376365966329544 1105 80-81 0.3503550944106628 1105 82-83 0.2139853319072138 1105 84-85 0.16013439926136996 1105 86-87 0.24776811109410346 1105 88-89 0.3293159110012631 1105 90-91 0.48512342894507654 1105 92-93 0.3660577268429357 1105 94-95 0.39700543432610047 1105 96-97 0.3454573223711428 1105 98-99 0.4867602811165206 1105 100-101 0.8061510737698825 1106 1 0.08739056110040977 1106 2 0.1359255316111856 1106 3 0.16338944767704078 1106 4 0.0963255762380939 1106 5 -0.0772888025629257 1106 6 0.027616601816355058 1106 7 0.09938815654916766 1106 8 0.11127978201894706 1106 9 -4.93593177850471E-4 1106 10-11 0.14092653969765934 1106 12-13 0.07040338221452203 1106 14-15 0.27290857220452835 1106 16-17 0.13103995237928956 1106 18-19 0.09778650222673946 1106 20-21 0.07673590054314872 1106 22-23 0.0018379116471365364 1106 24-25 -0.06256951067736338 1106 26-27 0.07040775374847641 1106 28-29 0.1041660706675458 1106 30-31 0.2028214384440048 1106 32-33 0.18440882763038502 1106 34-35 0.08729708630744426 1106 36-37 0.07121539801049437 1106 38-39 0.11238899081990184 1106 40-41 0.26440938620770993 1106 42-43 0.24794582853940028 1106 44-45 0.23493038221344875 1106 46-47 0.1671287613171586 1106 48-49 0.1667988483516467 1106 50-51 0.36088216468144196 1106 52-53 0.2507684431299211 1106 54-55 0.1043228327896557 1106 56-57 0.19734202740455942 1106 58-59 0.20286634448900998 1106 60-61 0.28139091167448527 1106 62-63 0.2220440872495857 1106 64-65 0.14200667721962645 1106 66-67 0.29484041392911564 1106 68-69 0.24592409531816983 1106 70-71 0.3539407299992199 1106 72-73 0.12195571874366706 1106 74-75 0.2626619842352298 1106 76-77 -0.08438168982751648 1106 78-79 -0.003373060544443973 1106 80-81 0.14886655791064385 1106 82-83 0.33918904944681927 1106 84-85 0.1585663386337295 1106 86-87 0.27414071693296194 1106 88-89 0.2662926131473249 1106 90-91 0.42281065007723484 1106 92-93 0.3535338017181999 1106 94-95 0.6358025019417859 1106 96-97 0.6240932624665909 1106 98-99 0.9748043522242789 1106 100-101 1.3075701721292 1107 1 -0.02079098604295737 1107 2 0.08183606433307489 1107 3 0.06569728420900134 1107 4 0.0505544327240699 1107 5 0.028265404009729878 1107 6 0.03903791069177487 1107 7 -0.0028409950054779642 1107 8 -0.11534017568787647 1107 9 0.007748043469675281 1107 10-11 -0.004169250041321959 1107 12-13 -0.0781248091375133 1107 14-15 0.094478168452234 1107 16-17 0.10497947252387263 1107 18-19 -0.003933502199288341 1107 20-21 0.04843059361027002 1107 22-23 0.037173997930125324 1107 24-25 0.09315021097452814 1107 26-27 0.005599994057320146 1107 28-29 -0.14886929454139874 1107 30-31 -0.15788125528700903 1107 32-33 -0.06020201983140794 1107 34-35 -0.011064129990174365 1107 36-37 -0.041709915807324194 1107 38-39 0.05320205867340633 1107 40-41 0.17126097281651198 1107 42-43 -0.0749407421479873 1107 44-45 -0.002422775021265977 1107 46-47 0.00924189863474112 1107 48-49 -0.0661112202518197 1107 50-51 0.026344070087347404 1107 52-53 -0.020017684339741493 1107 54-55 0.03899593666611878 1107 56-57 -0.18907828254049264 1107 58-59 0.006883504576052246 1107 60-61 0.07097114576229302 1107 62-63 0.2562419307894217 1107 64-65 0.12945142868574777 1107 66-67 0.22423278763358212 1107 68-69 0.26458288529075347 1107 70-71 -0.0536051900617629 1107 72-73 0.024522508765432605 1107 74-75 0.023769091086972338 1107 76-77 -0.1164310646507829 1107 78-79 0.08073779921187807 1107 80-81 0.251001041601949 1107 82-83 0.34210692507537743 1107 84-85 0.16649292800879678 1107 86-87 0.21377527746506786 1107 88-89 0.1579292606755125 1107 90-91 0.19870907268932925 1107 92-93 0.17097912180622998 1107 94-95 0.3442526845155207 1107 96-97 0.511498364080861 1107 98-99 0.6448435871358207 1107 100-101 0.9312991484868256 1108 1 -0.06680622350750198 1108 2 0.0017100538124132925 1108 3 0.02818135328050886 1108 4 -0.1265576647857074 1108 5 -0.14286353655228368 1108 6 -0.040798844146856084 1108 7 0.017876865127220753 1108 8 0.08385874850589659 1108 9 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-0.8703822780086341 2316 76-77 -0.9152518934865022 2316 78-79 -1.0467448821386363 2316 80-81 -1.6311861979351256 2316 82-83 -1.262419066562483 2316 84-85 -1.0395282916067075 2316 86-87 -1.2812596160269472 2316 88-89 -1.0299176247003068 2316 90-91 -1.384490812536189 2316 92-93 -1.323072417401555 2316 94-95 -1.588477684265822 2316 96-97 -1.5673060171617088 2316 98-99 -1.749160727154969 2316 100-101 -1.7848605690859678 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 14.0 4 29.0 5 76.0 6 180.0 7 384.0 8 982.0 9 1909.0 10 2943.0 11 4735.0 12 6686.0 13 9086.0 14 12208.0 15 16099.0 16 20062.0 17 25477.0 18 22935.0 19 15978.0 20 15072.0 21 15593.0 22 17066.0 23 19195.0 24 22006.0 25 26713.0 26 32777.0 27 40187.0 28 49386.0 29 61461.0 30 74784.0 31 91770.0 32 113619.0 33 142999.0 34 186198.0 35 246910.0 36 342774.0 37 417936.0 38 228880.0 39 12203.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.558615983734875 19.013284434607026 14.645754942308706 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29.172581185062725 21.190038101033686 20-21 22.803227074412586 25.51787675427771 28.7807320987606 22.898164072549104 22-23 21.81585930467214 25.815509324957038 29.31208526968462 23.056546100686205 24-25 23.481069659873302 25.813664877457843 29.112746273763314 21.59251918890554 26-27 22.688355903494983 24.979834207365727 30.891491276145338 21.440318612993952 28-29 21.080983194007104 27.965469934635262 29.622105127293757 21.33144174406388 30-31 21.793996737016407 25.859232032260703 29.19062249160313 23.15614873911976 32-33 21.899981761205865 26.194129545932377 29.724576796858575 22.18131189600318 34-35 23.69072671186541 25.559854669816122 29.108699002313337 21.64071961600513 36-37 22.99252738832984 24.977634405793506 30.315686825086907 21.71415138078975 38-39 21.93139499627031 26.396468774956666 30.28958387432189 21.382552354451132 40-41 22.14276075515721 26.743351891593065 29.32981321307465 21.78407414017508 42-43 21.440792304526383 27.031119377825735 28.07908941741789 23.448998900229988 44-45 22.51340658938847 25.575804825218647 28.38453400090411 23.526254584488772 46-47 23.70065940179153 26.980256687224774 27.901268853848382 21.417815057135314 48-49 23.398134809211776 26.572667853132714 28.044548847265606 21.984648490389908 50-51 21.399642882949706 27.142223475875447 29.528669917459123 21.929463723715724 52-53 22.894443185698794 28.10125364537457 27.042862574792892 21.96144059413375 54-55 21.730214259145153 26.48916102203447 28.078589038200818 23.70203568061956 56-57 23.148556036509135 26.4099668826895 27.588878712638554 22.85259836816281 58-59 24.899506789399446 25.915419202892792 27.011317007897766 22.173756999809992 60-61 22.790457546896718 26.784965713039494 29.331947315657253 21.092629424406535 62-63 22.152209651156742 26.936629336544186 28.83249720911028 22.078663803188785 64-65 21.648451869184306 28.522109158186865 27.678990041900203 22.15044893072863 66-67 22.39322389371459 26.974421791982888 26.84570258724731 23.786651727055215 68-69 21.941579114273257 26.973501067692652 27.72287743985346 23.362042378180632 70-71 23.445085628297058 26.841292414224792 27.145843861937834 22.567778095540312 72-73 23.061930511678423 25.723411363594316 29.00636292505199 22.20829519967527 74-75 21.81952812441437 28.860224939594797 27.21595842232776 22.10428851366308 76-77 22.447228602453333 26.78605903118237 27.13356246301274 23.633149903351562 78-79 22.749759329347803 27.006065792815814 27.649493857048924 22.59468102078746 80-81 23.641664129172145 26.8994588443782 27.11387078301603 22.34500624343362 82-83 23.025260162962617 26.4735022509875 28.275641712091254 22.225595873958632 84-85 22.31932352429044 27.47927912240989 27.904775378721926 22.296621974577747 86-87 22.392624884181593 27.91625684490313 27.11557811694345 22.575540153971822 88-89 21.944308948663753 27.95281220676591 26.148269604606988 23.954609239963354 90-91 22.571154742368865 26.52522024410838 26.87817240651992 24.02545260700284 92-93 23.542068635292708 27.343399552733388 27.221821387179446 21.89271042479446 94-95 22.837244095876347 26.81213343742784 27.49940527273333 22.85121719396249 96-97 21.217594719694908 26.939767983296854 28.97431605871523 22.868321238293007 98-99 22.10148105568993 27.889018440119507 26.580865396900162 23.4286351072904 100-101 21.354536564386173 26.70705156235002 27.330433171972224 24.60797870129158 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 371.0 1 288.0 2 266.5 3 354.0 4 327.0 5 293.0 6 512.5 7 1076.5 8 1681.5 9 2071.0 10 2056.0 11 1918.5 12 2020.0 13 2229.5 14 2500.5 15 2784.5 16 3056.0 17 3291.5 18 3642.5 19 4052.0 20 4486.5 21 5060.0 22 5769.0 23 6841.5 24 8313.0 25 10042.5 26 12456.0 27 15629.5 28 18508.0 29 21621.5 30 25367.0 31 29445.0 32 35039.0 33 39001.5 34 41765.5 35 45087.0 36 49419.0 37 54003.0 38 56307.0 39 56829.5 40 57132.0 41 59717.5 42 62675.5 43 65697.0 44 68227.5 45 71318.0 46 75452.5 47 104555.5 48 168315.5 49 163189.0 50 102674.5 51 78463.5 52 70951.5 53 69110.5 54 68462.5 55 63796.5 56 57632.5 57 51858.0 58 47227.0 59 42009.0 60 35454.5 61 29543.5 62 24912.5 63 19941.5 64 14953.0 65 11672.0 66 8802.0 67 6821.5 68 5032.0 69 3387.0 70 2516.0 71 1994.0 72 1735.5 73 1567.5 74 1371.5 75 1201.5 76 849.5 77 479.5 78 316.0 79 222.5 80 161.5 81 123.0 82 84.5 83 44.5 84 24.0 85 16.0 86 11.0 87 6.0 88 2.0 89 1.5 90 2.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03769621292353284 2 0.005702313964183374 3 0.0037435038238150396 4 9.576405130689636E-4 5 6.094075992257042E-4 6 2.611746853824446E-4 7 0.0 8 0.0 9 0.001610577226525075 10-11 3.482329138432595E-4 12-13 9.794050701841673E-4 14-15 0.002154691154405168 16-17 0.002785863310746076 18-19 0.0 20-21 0.0 22-23 0.001610577226525075 24-25 9.576405130689636E-4 26-27 0.004614086108423188 28-29 0.005506432950146541 30-31 0.0012623443126818156 32-33 5.223493707648892E-4 34-35 0.00866229373185108 36-37 0.011230511471445118 38-39 0.008988762088579136 40-41 0.0018717519119075198 42-43 6.311721563409078E-4 44-45 0.001240579755566612 46-47 1.5235189980642604E-4 48-49 6.311721563409078E-4 50-51 0.0011752860842210006 52-53 0.0022852784970963905 54-55 0.0031123316674741323 56-57 0.001088227855760186 58-59 8.052886132625375E-4 60-61 0.0036564455953542246 62-63 0.004635850665538392 64-65 0.008183473475316599 66-67 0.0028076278678612794 68-69 0.003025273439013317 70-71 0.0 72-73 8.923468417233525E-4 74-75 0.0051582000363032815 76-77 0.003025273439013317 78-79 0.0037652683809302435 80-81 0.00433114686592554 82-83 0.004309382308810336 84-85 0.00491878990803604 86-87 0.0032429190101653537 88-89 0.008422883603583838 90-91 0.01129580514279073 92-93 0.0011099924128753897 94-95 0.0018064582405619084 96-97 9.358759559537599E-4 98-99 0.0028946860963220944 100-101 0.0036782101524694285 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2297313.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.656051739493485 #Duplication Level Percentage of deduplicated Percentage of total 1 75.13763333669657 35.05625308540006 2 13.973418979214024 13.038891177436597 3 4.668358890734427 6.534215818338895 4 2.0429812204337376 3.8126975009348003 5 1.1196171024367136 2.6118456729854547 6 0.65427238011274 1.8315459610956926 7 0.4259059901974431 1.3909764340368478 8 0.2895309211494796 1.0806695709866663 9 0.23643096037573083 0.9927841608097409 >10 1.250727731695848 10.918824602055194 >50 0.11737992981739188 3.8333050020128776 >100 0.07327774784215887 6.2711034938561685 >500 0.006166762619523745 1.9072136087989762 >1k 0.0029899455124963556 3.0217674450541363 >5k 7.474863781240889E-4 2.900054521722325 >10k+ 5.606147835930667E-4 4.797851944475571 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 40494 1.762667951646119 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 19466 0.8473377376091112 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 14392 0.6264710120040238 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 14377 0.6258180752905678 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 11071 0.4819108236448408 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 10259 0.44656518288974983 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 9792 0.4262370865441496 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 9403 0.4093042611085211 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8937 0.38901969387715124 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8550 0.37217392666998356 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 8388 0.36512221016465757 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 8091 0.35219406323822655 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6778 0.2950403362537016 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 6586 0.2866827463214634 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4967 0.21620911038243373 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4707 0.2048915406825278 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3944 0.17167882652472694 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3617 0.1574448061713837 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3612 0.15722716060023165 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3564 0.15513776311717212 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3397 0.14786840104069407 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3163 0.1376825883107787 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2787 0.12131564136014554 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2634 0.1146556868828932 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2493 0.10851808177640575 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2338 0.1017710690706926 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2328 0.10133577792838852 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.352911423040744E-5 0.0 10-11 0.0 0.0 0.0 4.352911423040744E-5 0.0 12-13 0.0 0.0 0.0 4.352911423040744E-5 0.0 14-15 0.0 0.0 0.0 4.352911423040744E-5 2.176455711520372E-5 16-17 0.0 0.0 0.0 4.352911423040744E-5 4.352911423040744E-5 18-19 0.0 0.0 0.0 4.352911423040744E-5 4.352911423040744E-5 20-21 0.0 0.0 0.0 6.529367134561116E-5 4.352911423040744E-5 22-23 0.0 0.0 0.0 2.1764557115203719E-4 6.529367134561116E-5 24-25 0.0 0.0 0.0 3.482329138432595E-4 8.705822846081487E-5 26-27 0.0 0.0 0.0 3.699974709584632E-4 8.705822846081487E-5 28-29 0.0 0.0 0.0 8.270531703777413E-4 8.705822846081487E-5 30-31 0.0 0.0 0.0 0.0019152810261379273 8.705822846081487E-5 32-33 0.0 0.0 0.0 0.004200559523234317 8.705822846081487E-5 34-35 0.0 0.0 0.0 0.006834070934173968 8.705822846081487E-5 36-37 0.0 0.0 0.0 0.009358759559537598 8.705822846081487E-5 38-39 0.0 0.0 0.0 0.012579914012587748 8.705822846081487E-5 40-41 0.0 0.0 0.0 0.016845767207167676 8.705822846081487E-5 42-43 0.0 0.0 0.0 0.02263513939981187 8.705822846081487E-5 44-45 0.0 0.0 0.0 0.02831568880688004 8.705822846081487E-5 46-47 0.0 0.0 0.0 0.034888585055671564 1.305873426912223E-4 48-49 0.0 0.0 0.0 0.0409608964908134 1.305873426912223E-4 50-51 0.0 0.0 0.0 0.051016121878037515 1.305873426912223E-4 52-53 0.0 0.0 0.0 0.07421713976284469 1.5235189980642604E-4 54-55 0.0 0.0 0.0 0.10810455519121687 1.7411645692162975E-4 56-57 0.0 0.0 0.0 0.1398155149080687 1.7411645692162975E-4 58-59 0.0 0.0 0.0 0.1813422898838774 1.9588101403683345E-4 60-61 0.0 0.0 0.0 0.22900666996617353 2.1764557115203719E-4 62-63 0.0 0.0 0.0 0.258758819542657 2.1764557115203719E-4 64-65 0.0 0.0 0.0 0.2840709994676389 2.1764557115203719E-4 66-67 0.0 0.0 0.0 0.35933283797201343 2.1764557115203719E-4 68-69 0.0 0.0 0.0 0.44293050185151084 2.1764557115203719E-4 70-71 0.0 0.0 0.0 0.47444558055432584 2.1764557115203719E-4 72-73 0.0 0.0 0.0 0.5372145632745734 2.1764557115203719E-4 74-75 0.0 0.0 0.0 0.6313027436835991 2.1764557115203719E-4 76-77 0.0 0.0 0.0 0.7220827114111137 2.1764557115203719E-4 78-79 0.0 0.0 0.0 0.8159750108061026 2.1764557115203719E-4 80-81 0.0 0.0 0.0 0.9420135610602474 2.1764557115203719E-4 82-83 0.0 0.0 0.0 1.0908396026140104 2.1764557115203719E-4 84-85 0.0 0.0 0.0 1.2469350062442515 2.1764557115203719E-4 86-87 0.0 0.0 0.0 1.4665829166508875 2.1764557115203719E-4 88-89 0.0 0.0 0.0 1.7453215996253015 2.1764557115203719E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9980 0.0 23.753668 1 TACATGG 10325 0.0 22.77046 2 GAGTACT 10445 0.0 21.966534 12-13 ACATGGG 10385 0.0 21.908094 3 ACTACCG 110 0.0028781972 21.590954 8 GTACTTT 10830 0.0 21.097454 14-15 CATGGGG 4360 0.0 20.26292 4 AGTACTT 10915 0.0 19.693264 12-13 TAAGGTG 920 0.0 19.618752 5 GTACCTG 1475 0.0 19.325018 1 CGCGGAA 275 0.0 19.00066 82-83 TACCTGG 1450 0.0 18.670828 2 GTAAGGT 745 0.0 18.489166 4 CGTATAC 130 0.007567178 18.268473 3 ACTTTTT 12810 0.0 18.001986 16-17 ATGGGGA 2460 0.0 17.377329 5 CATGGGA 5305 0.0 17.369684 4 TACTTTT 12275 0.0 17.220745 14-15 ATTCGCG 310 0.0 16.85524 80-81 AGAGTAC 18825 0.0 16.74319 10-11 >>END_MODULE