##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936424_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2370355 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.219487376363457 31.0 31.0 34.0 28.0 34.0 2 31.24086476498246 31.0 31.0 34.0 28.0 34.0 3 31.187007009498576 31.0 31.0 34.0 27.0 34.0 4 34.83430878497103 37.0 35.0 37.0 32.0 37.0 5 34.87919531040709 37.0 35.0 37.0 32.0 37.0 6 34.96094888740294 37.0 35.0 37.0 32.0 37.0 7 34.83097890400383 37.0 35.0 37.0 32.0 37.0 8 34.819559939333985 37.0 35.0 37.0 32.0 37.0 9 36.26188819818128 39.0 35.0 39.0 32.0 39.0 10-11 36.12656817227799 39.0 35.0 39.0 31.0 39.0 12-13 36.03254470321956 39.0 35.0 39.0 30.5 39.0 14-15 36.93226035762576 39.0 36.0 41.0 31.0 41.0 16-17 36.87867007262625 39.0 36.0 41.0 31.0 41.0 18-19 36.77756770610309 39.0 36.0 41.0 30.0 41.0 20-21 36.742801816605535 39.0 36.0 41.0 30.0 41.0 22-23 36.75234954257907 39.0 36.0 41.0 30.0 41.0 24-25 36.853309947244185 39.0 36.0 41.0 30.0 41.0 26-27 36.76300364291424 39.0 36.0 41.0 30.0 41.0 28-29 36.668832305709486 39.0 36.0 41.0 30.0 41.0 30-31 36.503016003931904 39.0 36.0 41.0 30.0 41.0 32-33 36.31576873506289 39.0 35.0 40.0 30.0 41.0 34-35 36.07003339162277 38.5 35.0 40.0 29.0 41.0 36-37 35.82887204659218 38.0 35.0 40.0 28.0 41.0 38-39 35.62447418213728 38.0 35.0 40.0 27.0 41.0 40-41 35.45653541347182 38.0 34.5 40.0 27.0 41.0 42-43 35.15160239710929 38.0 34.0 40.0 26.0 41.0 44-45 34.971078804651626 38.0 34.0 40.0 25.0 41.0 46-47 34.786274629749556 38.0 34.0 40.0 24.0 41.0 48-49 34.493764436128764 38.0 33.0 40.0 24.0 41.0 50-51 33.47134121260318 37.0 32.0 39.5 22.5 40.5 52-53 33.59807286250371 37.5 33.0 39.5 23.0 40.0 54-55 34.0501549345984 38.0 33.0 40.0 23.0 41.0 56-57 33.82752435816576 38.0 33.0 40.0 22.0 41.0 58-59 33.58101065030344 37.5 33.0 40.0 21.0 41.0 60-61 33.19039004706046 37.0 32.0 40.0 20.0 41.0 62-63 32.89213640994703 36.5 32.0 39.5 18.5 41.0 64-65 32.542259070898666 36.0 31.5 39.0 18.0 41.0 66-67 32.08408170927983 35.5 31.0 39.0 13.5 40.5 68-69 31.621091777391996 35.0 31.0 38.5 12.0 40.0 70-71 31.15085061098443 35.0 30.0 37.5 8.0 40.0 72-73 30.715866610697553 35.0 30.0 37.0 7.0 39.0 74-75 30.320898346450214 34.5 29.5 36.5 6.0 39.0 76-77 29.86850640515872 34.0 29.0 36.0 2.0 39.0 78-79 29.365143617728144 34.0 29.0 35.5 2.0 37.5 80-81 28.831061381101144 34.0 28.0 35.0 2.0 37.0 82-83 28.408971651925555 34.0 27.5 35.0 2.0 36.5 84-85 28.02389810808929 33.5 26.5 35.0 2.0 36.0 86-87 27.74922617076345 33.0 26.0 35.0 2.0 36.0 88-89 27.339580358216384 33.0 26.0 35.0 2.0 35.5 90-91 26.910837406211307 33.0 25.0 35.0 2.0 35.0 92-93 26.45632468554288 33.0 25.0 35.0 2.0 35.0 94-95 25.919914949448497 32.5 23.5 35.0 2.0 35.0 96-97 25.257037026099468 32.0 19.5 35.0 2.0 35.0 98-99 24.5090231210093 32.0 9.5 34.5 2.0 35.0 100-101 21.889416353246666 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5317839620571156 1101 2 0.45642348925165876 1101 3 0.6654945572365314 1101 4 0.7114214741830835 1101 5 0.5406832230799381 1101 6 0.653881241621562 1101 7 0.6211094131498314 1101 8 0.5907578738370631 1101 9 0.805413960994521 1101 10-11 0.8630496350094106 1101 12-13 0.906858463978466 1101 14-15 1.0939195675352096 1101 16-17 1.2239421001854254 1101 18-19 1.3074067874376496 1101 20-21 1.1839132260739476 1101 22-23 1.155162769327795 1101 24-25 1.1714525160587712 1101 26-27 1.0699744717103457 1101 28-29 1.0990014333837976 1101 30-31 0.8683831203493568 1101 32-33 0.9390278913790127 1101 34-35 0.9612146304408569 1101 36-37 0.9328799467784776 1101 38-39 0.9517914439896487 1101 40-41 0.7998475052275822 1101 42-43 0.9507915895624635 1101 44-45 0.9763661685536533 1101 46-47 1.1623686774973905 1101 48-49 1.2115392190788725 1101 50-51 1.0816695222413344 1101 52-53 1.0297561991634723 1101 54-55 1.0803175549020239 1101 56-57 1.1999206238903781 1101 58-59 1.2320181706305746 1101 60-61 1.1886072446352216 1101 62-63 1.1652415090800687 1101 64-65 1.038419347671919 1101 66-67 1.218789331683105 1101 68-69 1.1958080853488227 1101 70-71 1.18658001533354 1101 72-73 1.35567970137339 1101 74-75 1.2329757592432244 1101 76-77 1.362855319062998 1101 78-79 1.3624271968396613 1101 80-81 1.3481297645033976 1101 82-83 1.4626522361933318 1101 84-85 1.452443552520652 1101 86-87 1.411068639347203 1101 88-89 1.450014643809343 1101 90-91 1.4014182850639436 1101 92-93 1.555335462485921 1101 94-95 1.6265210687704048 1101 96-97 1.6079211569424636 1101 98-99 1.4764015207854229 1101 100-101 1.788217814441925 1102 1 0.2996717460333542 1102 2 0.4399147607045144 1102 3 0.48323504714037213 1102 4 0.4148611811409779 1102 5 0.46458582782053526 1102 6 0.3539960979175021 1102 7 0.3257943536400063 1102 8 0.27514696466575117 1102 9 0.39817511766135283 1102 10-11 0.5020733170715488 1102 12-13 0.467394039520606 1102 14-15 0.5797309327379523 1102 16-17 0.37747253864646524 1102 18-19 0.42993596729582606 1102 20-21 0.585995362808994 1102 22-23 0.3769320053577374 1102 24-25 0.4673425426551674 1102 26-27 0.43236999593138137 1102 28-29 0.22557963619214405 1102 30-31 0.15742727634754772 1102 32-33 0.394626874418293 1102 34-35 -0.0013396631980668872 1102 36-37 0.13608800430500878 1102 38-39 -0.018906745511067413 1102 40-41 0.23733103994936755 1102 42-43 0.18028384451841362 1102 44-45 0.4134419385716086 1102 46-47 0.373623703210221 1102 48-49 0.6178885731600445 1102 50-51 0.4275192951426092 1102 52-53 0.14714966913483352 1102 54-55 0.47957661952509767 1102 56-57 0.31417144323347657 1102 58-59 0.26977017187426355 1102 60-61 0.40403770207042555 1102 62-63 0.49222140897118294 1102 64-65 0.4964361234443544 1102 66-67 0.4308061523627984 1102 68-69 0.4758413955600673 1102 70-71 0.33346246648790157 1102 72-73 0.708585226285166 1102 74-75 0.5309575724917899 1102 76-77 0.48331765121999837 1102 78-79 0.4215559070228281 1102 80-81 0.6456586486935905 1102 82-83 0.6355356434265182 1102 84-85 0.6627666670545409 1102 86-87 0.5214196409417546 1102 88-89 0.6954396318155069 1102 90-91 0.815923447426794 1102 92-93 0.7659716422705607 1102 94-95 0.9939974543262444 1102 96-97 0.8517712201982697 1102 98-99 1.096238493748686 1102 100-101 1.5030479373352499 1103 1 0.2937282464029174 1103 2 0.43471610227536317 1103 3 0.4091193021492501 1103 4 0.5983196144805518 1103 5 0.43576023536242303 1103 6 0.5052469314019419 1103 7 0.4623644384376817 1103 8 0.32263737334798037 1103 9 0.30490100991210056 1103 10-11 0.30258580589298134 1103 12-13 0.2953316765606786 1103 14-15 0.6885731734677734 1103 16-17 0.7552321990940598 1103 18-19 0.9409738951809103 1103 20-21 0.7481763244775124 1103 22-23 0.6739684892503703 1103 24-25 0.6271611916702895 1103 26-27 0.6538098572624165 1103 28-29 0.6519029429634458 1103 30-31 0.5519610938038682 1103 32-33 0.5788967801209282 1103 34-35 0.6119997050147745 1103 36-37 0.5520439782408033 1103 38-39 0.3863404108851256 1103 40-41 0.5652175137759343 1103 42-43 0.579312647741844 1103 44-45 0.651700457008701 1103 46-47 0.6721346395255878 1103 48-49 0.7955895981852734 1103 50-51 0.8750446375910883 1103 52-53 0.8840210695647954 1103 54-55 0.8299070862906675 1103 56-57 0.954327950313747 1103 58-59 0.8070089003803602 1103 60-61 0.8710769321187968 1103 62-63 0.8298814843881388 1103 64-65 0.8350154709253914 1103 66-67 0.7787676433587833 1103 68-69 0.9459075841005529 1103 70-71 0.8240136918111745 1103 72-73 0.8403500144766056 1103 74-75 0.751992411546599 1103 76-77 0.7680097605198526 1103 78-79 0.7644953121683713 1103 80-81 0.9547589423942355 1103 82-83 0.7734014663523041 1103 84-85 0.9846020795180905 1103 86-87 0.9193487920274386 1103 88-89 0.7079217901320973 1103 90-91 0.9199370706437584 1103 92-93 0.9278403893524718 1103 94-95 1.0559634829043496 1103 96-97 1.2845372843764515 1103 98-99 1.5689442281692507 1103 100-101 2.174618419540206 1104 1 0.35335902796229846 1104 2 0.3816287109350789 1104 3 0.6603640151510426 1104 4 0.620001248248343 1104 5 0.5554552694471653 1104 6 0.41085806706892214 1104 7 0.4690133889438286 1104 8 0.4828783382858006 1104 9 0.589411587502056 1104 10-11 0.5815800723972231 1104 12-13 0.6069748414647123 1104 14-15 0.6409522328223005 1104 16-17 0.573854695743087 1104 18-19 0.8062030553342865 1104 20-21 0.8028183717900816 1104 22-23 0.4294299988253627 1104 24-25 0.5080649314691286 1104 26-27 0.2708497132332681 1104 28-29 0.29981738612619324 1104 30-31 0.32889266737984 1104 32-33 0.4160419627986158 1104 34-35 0.4684603988558038 1104 36-37 0.5682888080326975 1104 38-39 0.32299615318505914 1104 40-41 0.6237185756675032 1104 42-43 0.6508537740296774 1104 44-45 0.6846974797594072 1104 46-47 0.780895200046217 1104 48-49 0.9381002530363816 1104 50-51 0.8915603305876374 1104 52-53 0.7071772682445285 1104 54-55 0.815466888214651 1104 56-57 0.8894043310423001 1104 58-59 0.7797561540161269 1104 60-61 0.8607901944437444 1104 62-63 0.872412847023206 1104 64-65 0.8019838603359375 1104 66-67 0.8012113325605483 1104 68-69 0.6252634956562986 1104 70-71 0.5853872512501468 1104 72-73 0.732406760093383 1104 74-75 0.6863105549792223 1104 76-77 0.5958685576895775 1104 78-79 0.6549437232839175 1104 80-81 0.7925844274325229 1104 82-83 0.8267913889833522 1104 84-85 0.7609146655384933 1104 86-87 0.9479192452089826 1104 88-89 0.9669007032294807 1104 90-91 0.9531393744633334 1104 92-93 0.8301207610684251 1104 94-95 0.9912637861992692 1104 96-97 0.8541567642472288 1104 98-99 0.771384153136097 1104 100-101 1.3276022270413677 1105 1 0.3777288380348942 1105 2 0.41934377920565424 1105 3 0.42664290610961686 1105 4 0.5101955858235598 1105 5 0.43933856825351825 1105 6 0.3327066822178537 1105 7 0.5032685056102082 1105 8 0.41126488378887416 1105 9 0.3431584902288165 1105 10-11 0.5131404162743394 1105 12-13 0.47704125512695583 1105 14-15 0.7067668229377233 1105 16-17 0.5969438885080862 1105 18-19 0.44103134888906226 1105 20-21 0.4944302014879298 1105 22-23 0.44179812897176163 1105 24-25 0.355653845697141 1105 26-27 0.33429811715831903 1105 28-29 0.4580679562565848 1105 30-31 0.2759582367268436 1105 32-33 0.38715970850596193 1105 34-35 0.22992444437338833 1105 36-37 0.35329822316305837 1105 38-39 0.0769003132509809 1105 40-41 0.11138286896694893 1105 42-43 -0.07468313502219104 1105 44-45 0.06879787783518054 1105 46-47 0.30381407905091606 1105 48-49 0.3551224170366396 1105 50-51 0.3538604222727244 1105 52-53 0.6109050940892828 1105 54-55 0.4539118104273925 1105 56-57 0.42004949233262323 1105 58-59 0.36764538043768624 1105 60-61 0.24956174479220294 1105 62-63 0.45764864598914556 1105 64-65 0.5503000897575703 1105 66-67 0.48405628320728056 1105 68-69 0.44753702477792245 1105 70-71 0.6443851564199115 1105 72-73 0.5407287778617231 1105 74-75 0.5716963577170091 1105 76-77 0.5722665377161569 1105 78-79 0.6098546059560164 1105 80-81 0.7030503820890246 1105 82-83 0.5329073793274013 1105 84-85 0.6893963053089323 1105 86-87 0.6219282628438556 1105 88-89 0.7657518832398083 1105 90-91 0.7442001506530644 1105 92-93 1.0006632988885222 1105 94-95 0.8944541227067653 1105 96-97 1.1105558613231707 1105 98-99 1.3496845262596011 1105 100-101 1.9640727282338553 1106 1 0.21634981849975077 1106 2 0.093762705100076 1106 3 0.1339298698383793 1106 4 0.25664225496210946 1106 5 0.37059552042259014 1106 6 0.17644736722007082 1106 7 0.31491834166624955 1106 8 0.38126931863659763 1106 9 0.489416860095119 1106 10-11 0.6433471819617083 1106 12-13 0.5534307801620244 1106 14-15 0.4272896260522927 1106 16-17 0.3530029083340409 1106 18-19 0.5362366241767802 1106 20-21 0.29465376143470934 1106 22-23 0.06355905432818787 1106 24-25 -0.05103732168564079 1106 26-27 0.008304456809490546 1106 28-29 0.20014121121671025 1106 30-31 0.16933213801149805 1106 32-33 0.1895876058792183 1106 34-35 0.28621102277931953 1106 36-37 0.12040619352955417 1106 38-39 0.20913802108903212 1106 40-41 0.15020632567628667 1106 42-43 0.2380132523944738 1106 44-45 0.14085542907221793 1106 46-47 0.29258409948029396 1106 48-49 0.2828245842958239 1106 50-51 0.25068587102843054 1106 52-53 0.37535760850253297 1106 54-55 0.5149967486666824 1106 56-57 0.611434982383301 1106 58-59 0.35057630586680233 1106 60-61 0.5724764575812884 1106 62-63 0.3855187558424973 1106 64-65 0.2695200654749641 1106 66-67 0.12097784456448579 1106 68-69 0.35751506864656335 1106 70-71 0.09270209535714713 1106 72-73 0.25342770986314633 1106 74-75 0.39020348618945633 1106 76-77 0.34391550134313675 1106 78-79 0.49239802747738537 1106 80-81 0.48808822369952765 1106 82-83 0.47968120936367953 1106 84-85 0.6464400794370491 1106 86-87 0.8217127001634594 1106 88-89 0.7646958805643571 1106 90-91 1.1817102381140785 1106 92-93 1.0774835857722813 1106 94-95 1.0207265968428771 1106 96-97 0.9997875421304592 1106 98-99 1.1928919389985175 1106 100-101 1.7052771892544492 1107 1 0.1204937991410091 1107 2 0.213721366120442 1107 3 0.15994822031226263 1107 4 0.12926274296372497 1107 5 0.1763174401644747 1107 6 0.06920548377582492 1107 7 0.19187942252932544 1107 8 0.2968369734057106 1107 9 0.4124104071812269 1107 10-11 0.37626460482921686 1107 12-13 0.20218682976880586 1107 14-15 -0.009746570761578255 1107 16-17 0.11916292043324717 1107 18-19 0.4411620123598752 1107 20-21 0.23508908105559811 1107 22-23 0.2035393931061691 1107 24-25 0.11447487835469161 1107 26-27 -0.12576491201486562 1107 28-29 0.047476863565968586 1107 30-31 -0.004236121718292907 1107 32-33 0.17149424028131932 1107 34-35 0.2590003956367468 1107 36-37 0.052196877135124 1107 38-39 0.28421490150846296 1107 40-41 0.029909894944289306 1107 42-43 0.018827176572735027 1107 44-45 0.04984917781187903 1107 46-47 0.17721809827037305 1107 48-49 0.18477054114196534 1107 50-51 0.2631417593123544 1107 52-53 0.24317370045655906 1107 54-55 0.2975726208183289 1107 56-57 0.5008809392294324 1107 58-59 0.6175441925375083 1107 60-61 0.5089908758107669 1107 62-63 0.6283966041990183 1107 64-65 0.5763296541016985 1107 66-67 0.5149131137719891 1107 68-69 0.5470353852426513 1107 70-71 0.5337297723081456 1107 72-73 0.47206554007084733 1107 74-75 0.5318109171231136 1107 76-77 0.6448267233637033 1107 78-79 0.7601649663763581 1107 80-81 0.6853848056716991 1107 82-83 0.8259329738323267 1107 84-85 0.7799473389651794 1107 86-87 1.019184468665177 1107 88-89 0.9627310186970455 1107 90-91 0.9665169027642087 1107 92-93 1.0474674534875454 1107 94-95 1.111161916463768 1107 96-97 1.2396002563873658 1107 98-99 1.4187867766674032 1107 100-101 1.863189570782982 1108 1 0.10694799543647804 1108 2 0.14640591042889284 1108 3 0.28481177558816384 1108 4 0.4137124986036227 1108 5 0.3258543738342965 1108 6 0.34814600176961363 1108 7 0.491746022439564 1108 8 0.38989791345347413 1108 9 -0.027549685556081727 1108 10-11 0.21757953165650434 1108 12-13 0.16303924562976846 1108 14-15 0.5674102425662539 1108 16-17 0.7141855353005369 1108 18-19 0.6926225911110393 1108 20-21 0.6370758882039453 1108 22-23 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0.3751816481347987 2313 50-51 0.3014983609102515 2313 52-53 0.48285484286657265 2313 54-55 0.3651213709212868 2313 56-57 0.44706278174652425 2313 58-59 0.439832821974953 2313 60-61 0.41458967327390894 2313 62-63 0.35495459983365407 2313 64-65 0.3742187797915193 2313 66-67 0.5699294324442619 2313 68-69 0.2910317096805066 2313 70-71 0.15746468911895306 2313 72-73 0.2833493614199938 2313 74-75 0.4410222156158241 2313 76-77 0.3341657913976377 2313 78-79 0.20893231461785433 2313 80-81 0.20163140809324887 2313 82-83 -0.004004068009368922 2313 84-85 -0.0838522347931594 2313 86-87 0.008491105268422672 2313 88-89 0.19426440762712005 2313 90-91 0.08569983662468061 2313 92-93 0.20235070033706393 2313 94-95 0.18843880881201258 2313 96-97 0.2607102864507844 2313 98-99 0.38725493209117445 2313 100-101 0.3217108005004725 2314 1 -0.48857750205348793 2314 2 -0.38288816594367603 2314 3 -0.5523103150095743 2314 4 -0.2451569448133597 2314 5 -0.3170388565967315 2314 6 -0.24683616421125976 2314 7 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2315 90-91 -1.6553997783062862 2315 92-93 -1.7511562609710118 2315 94-95 -1.7987862657734333 2315 96-97 -1.5442874441859082 2315 98-99 -1.7324912027639314 2315 100-101 -2.166876614795907 2316 1 -5.292312471401527 2316 2 -5.418404492931078 2316 3 -6.131689099873846 2316 4 -8.131336672240955 2316 5 -8.638066070314885 2316 6 -9.32745221887496 2316 7 -10.99447072900523 2316 8 -10.893384245700453 2316 9 -11.113916053739935 2316 10-11 -11.306121103968323 2316 12-13 -9.786501435907553 2316 14-15 -9.548797398865904 2316 16-17 -8.301329460153255 2316 18-19 -9.15757822326568 2316 20-21 -10.53091675622617 2316 22-23 -9.653327149771705 2316 24-25 -9.822220761291874 2316 26-27 -7.754627976000869 2316 28-29 -6.296870737492636 2316 30-31 -8.338463188777666 2316 32-33 -6.173028105729699 2316 34-35 -4.7709290127382005 2316 36-37 -5.186943591127996 2316 38-39 -4.396891567522992 2316 40-41 -3.8994411209775137 2316 42-43 -3.973297295190619 2316 44-45 -4.238079814376626 2316 46-47 -4.205692112170571 2316 48-49 -4.905441874020028 2316 50-51 -4.819305843880542 2316 52-53 -4.891888140581564 2316 54-55 -4.166457931535792 2316 56-57 -4.806991654359159 2316 58-59 -5.013399803706143 2316 60-61 -5.399057263088789 2316 62-63 -4.860895932190612 2316 64-65 -5.420927783897188 2316 66-67 -5.020837569200651 2316 68-69 -5.370639733210417 2316 70-71 -4.99719341461293 2316 72-73 -4.1277951975396014 2316 74-75 -4.740310993492841 2316 76-77 -5.1449564328236015 2316 78-79 -5.217514367298463 2316 80-81 -5.719252471828618 2316 82-83 -5.1604355859491235 2316 84-85 -5.1256543706007065 2316 86-87 -5.785977577264735 2316 88-89 -7.273134649122138 2316 90-91 -6.485374878086947 2316 92-93 -5.870943994841305 2316 94-95 -5.264003490972097 2316 96-97 -6.679081979921168 2316 98-99 -8.209654765143327 2316 100-101 -8.526623732243438 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6522.0 3 2497.0 4 2532.0 5 2721.0 6 3081.0 7 3662.0 8 4608.0 9 5895.0 10 7300.0 11 9031.0 12 11218.0 13 13531.0 14 15370.0 15 18020.0 16 21514.0 17 25684.0 18 22032.0 19 18442.0 20 18779.0 21 20258.0 22 22632.0 23 25108.0 24 28817.0 25 32886.0 26 37667.0 27 44544.0 28 52190.0 29 61809.0 30 74048.0 31 90323.0 32 110797.0 33 138161.0 34 177960.0 35 234175.0 36 311340.0 37 381615.0 38 274848.0 39 38722.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.85756372378073 18.144822514061158 14.009816844002025 21.987796918156093 2 15.422492326819059 21.14362785209593 38.338558509666335 25.095321311418683 3 22.052294509363442 23.644598520988943 29.598196089219996 24.70491088042762 4 12.44336177657302 15.870686359481928 38.436055034335276 33.249896829609774 5 13.09331607149036 38.56042812521363 33.852721820730586 14.493533982565426 6 31.091657502069946 36.168576813387425 16.802862561433745 15.936903123108879 7 28.27772298463503 33.150798985351095 20.3800373569139 18.191440673099983 8 26.14924115596552 31.942238870611117 19.47511141841403 22.433408555009333 9 25.264555513807206 18.393035813044342 21.720402416525587 34.62200625662286 10-11 25.745503067747528 25.485236840999942 26.511294912598377 22.25796517865416 12-13 28.082494580471636 23.292165386723152 26.520522460893194 22.10481757191202 14-15 23.358954353417282 25.058020309271413 27.211625292528513 24.371400044782792 16-17 21.81904640092394 28.535008482542928 27.23765852266563 22.408286593867498 18-19 21.36716167037316 28.49583132742194 28.534707842566576 21.602299159638328 20-21 23.052292125325565 25.83537543131958 27.96991021801484 23.142422225340017 22-23 22.029474403724013 25.966399053173543 28.669369172655536 23.334757370446905 24-25 23.65099231332728 26.026071222407136 28.288082281493583 22.034854182771994 26-27 22.70508018547057 25.24110077155623 30.257904189292063 21.79591485368114 28-29 21.229924966295062 28.297615202758074 28.773566298055247 21.698893532891613 30-31 21.876431103776923 26.141935119146936 28.4940764564589 23.48755732061724 32-33 22.038134998781715 26.496303476704362 28.962768959119373 22.50279256539455 34-35 23.864894713358527 25.865377819923612 28.24154484399772 22.028182622720145 36-37 22.964646489150596 25.274997885240158 29.739472134848477 22.020883490760767 38-39 22.14561545074337 26.71225521995264 29.395503378004207 21.74662595129978 40-41 22.297441540020603 27.050265498971843 28.721963975168908 21.93032898583865 42-43 21.606835068306594 27.266928250387977 27.31814527705395 23.808091404251478 44-45 22.565024748480973 26.024644065901732 27.630632217428037 23.779698968189265 46-47 23.75245665352584 27.111814992605517 27.468893287210722 21.666835066657917 48-49 23.26516939872959 26.785255867355712 27.60087742705212 22.348697306862583 50-51 21.355008782526834 27.363514343879746 29.128077654960922 22.1533992186325 52-53 22.760986553895794 28.16512062343483 26.677978629334376 22.395914193335 54-55 21.612799797498262 26.656654080621006 27.774801189697722 23.95574493218301 56-57 23.180806212970946 26.564032659611765 27.136563096056264 23.118598031361024 58-59 24.6304234098925 26.163470138549034 26.757914676747053 22.448191774811416 60-61 22.783168722022758 26.83013884728486 28.94814005391882 21.43855237677356 62-63 22.026452347804078 27.287600725646545 28.438109100113913 22.24783782643547 64-65 21.56635922099701 28.675768746011908 27.299783258713155 22.458088774277922 66-67 22.286758014754803 27.23208880387555 26.4486170168334 24.032536164536246 68-69 21.808609490990406 27.236808992357233 27.449336671055036 23.50524484559732 70-71 23.36847221185086 27.057084118568508 26.710378267137326 22.86406540244331 72-73 22.82988490177946 26.054926365452935 28.72153480047539 22.39365393229221 74-75 21.669477519018084 29.052848946890293 26.937895282704797 22.339778251386825 76-77 22.318734289067237 26.97547902388716 26.89823514294865 23.807551544096953 78-79 22.67377903279641 27.24051981063884 27.355526458702467 22.73017469786228 80-81 23.573915393441823 27.12632926234708 26.752035651350415 22.547719692860678 82-83 22.90390165758956 26.754277715126136 28.0943689882371 22.247451639047206 84-85 22.33521651688811 27.793565899681766 27.481577047745898 22.389640535684226 86-87 22.38788181776952 28.1723267614428 26.950277863033406 22.48951355775427 88-89 21.900919539841944 28.088195835491973 25.878912347551974 24.131972277114105 90-91 22.50450171461745 26.83591482266535 26.645425211626993 24.0141582510902 92-93 23.589349149125542 27.543937112058224 27.042253758827872 21.824459979988358 94-95 22.791746912982507 27.058372061476433 27.238263046737693 22.91161797880337 96-97 21.14702962380706 27.312394017603577 28.69962157507298 22.840954783516384 98-99 22.081662845696524 28.216334847796265 26.36336476614017 23.338637540367042 100-101 21.579661583370573 26.84452506604656 27.255910434069687 24.31990291651318 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 296.0 1 211.0 2 188.5 3 280.0 4 286.5 5 276.5 6 459.0 7 910.0 8 1479.0 9 1836.5 10 1810.0 11 1723.5 12 1843.0 13 2043.0 14 2275.5 15 2469.0 16 2640.0 17 2842.0 18 3104.5 19 3491.5 20 3919.0 21 4550.5 22 5379.5 23 6461.0 24 7850.0 25 9666.0 26 12203.0 27 15443.0 28 18318.5 29 21297.5 30 25379.5 31 30062.0 32 35735.5 33 39689.0 34 42980.0 35 46613.5 36 50933.0 37 55542.0 38 57632.5 39 58695.5 40 59473.5 41 61981.0 42 65032.5 43 68377.5 44 71751.0 45 76108.5 46 81338.0 47 108155.5 48 166337.5 49 162871.5 50 107582.0 51 83467.5 52 75499.0 53 73157.0 54 71916.5 55 67154.5 56 60621.5 57 54423.0 58 49591.5 59 44036.5 60 37001.5 61 30872.5 62 26286.5 63 21113.5 64 15924.5 65 12490.5 66 9382.5 67 7221.5 68 5406.5 69 3692.5 70 2780.5 71 2240.5 72 1890.5 73 1683.0 74 1417.0 75 1171.0 76 828.5 77 482.5 78 292.5 79 202.5 80 150.5 81 100.0 82 57.5 83 32.0 84 21.0 85 18.0 86 12.0 87 10.0 88 8.5 89 5.0 90 5.0 91 2.0 92 1.0 93 3.0 94 2.5 95 0.5 96 1.0 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07479892252426323 2 0.031134576888272012 3 0.016579795009608263 4 0.020629821271497307 5 0.02451109643914097 6 0.030754886926219912 7 0.0780895688620481 8 0.057248808722744064 9 0.07024264297963807 10-11 0.06051836117374823 12-13 0.038580718921849264 14-15 0.04815734352027439 16-17 0.05788162532616423 18-19 0.05549801611994828 20-21 0.018879028668701524 22-23 0.014027434709146941 24-25 0.022739209949564517 26-27 0.025270476363245167 28-29 0.006475823241666333 30-31 0.0010757882258142766 32-33 0.009386779617399081 34-35 0.011707107163273013 36-37 0.0046195612049671885 38-39 0.010230535088625965 40-41 0.004260965129695763 42-43 0.0020882947912865372 44-45 3.164083017100814E-4 46-47 2.7422052814873724E-4 48-49 6.53910490200835E-4 50-51 9.914126786915883E-4 52-53 1.2656332068403258E-4 54-55 2.1093886780672094E-4 56-57 0.0037125240733982888 58-59 0.0031640830171008136 60-61 0.005273471695168024 62-63 0.0023203275458739303 64-65 7.382860373235232E-4 66-67 3.375021884907535E-4 68-69 0.003185176903881486 70-71 0.0019406375838218325 72-73 0.021494670629504865 74-75 0.015778227311942724 76-77 0.02480641085407038 78-79 0.02075638459218134 80-81 0.012255548219570485 82-83 0.004471903997502484 84-85 0.003290646337784847 86-87 0.001371102640743686 88-89 0.0016453231688924235 90-91 0.006201602713517595 92-93 0.00989303290013521 94-95 0.013014928143674682 96-97 0.011306323314440243 98-99 0.022697022176003175 100-101 0.018393869272746066 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2370355.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.41831254462586 #Duplication Level Percentage of deduplicated Percentage of total 1 76.84338499767134 37.974704168019365 2 13.25913569510794 13.104882237048987 3 4.313553461685342 6.395055994425573 4 1.8950846661063614 3.7460754531268847 5 0.9619856056576139 2.3769852661909585 6 0.5957169961276901 1.766359722167032 7 0.36834562764545303 1.2742113546000566 8 0.27213965056168965 1.0758945845794277 9 0.1912168175683278 0.8504651208942298 >10 1.1160627334404298 10.354812183215675 >50 0.10842753283351386 3.6972595288970207 >100 0.06598104850329016 5.89594507038278 >500 0.005344618842793429 1.769362095535458 >1k 0.002413698832229272 2.634319190632277 >5k 7.758317675022661E-4 2.883999723254545 >10k+ 4.310176486123701E-4 4.199668307029687 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 35888 1.5140348175695202 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 23345 0.9848735737895801 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 14895 0.6283868871962217 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 14172 0.5978851269113697 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 10283 0.43381687553130227 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 9756 0.41158391886447393 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 8817 0.37196959949037167 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 8140 0.34340847678934167 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7362 0.3105863889586159 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 7293 0.30767543258288316 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 7233 0.30514416616920254 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 6951 0.29324721402490345 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6501 0.27426271592229856 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5646 0.23819216952734928 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4224 0.17820115552311785 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4096 0.1728011205072658 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3970 0.16748546103853643 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3895 0.16432137802143562 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3613 0.15242442587713656 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3501 0.147699395238266 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3062 0.1291789626448359 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2941 0.12407424204391325 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2923 0.12331486211980906 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2819 0.11892733366942927 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2412 0.10175690982996217 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.2187773561344186E-5 2 0.0 0.0 0.0 0.0 4.2187773561344186E-5 3 0.0 0.0 0.0 0.0 4.2187773561344186E-5 4 0.0 0.0 0.0 0.0 4.2187773561344186E-5 5 0.0 0.0 0.0 0.0 4.2187773561344186E-5 6 0.0 0.0 0.0 0.0 4.2187773561344186E-5 7 0.0 0.0 0.0 0.0 4.2187773561344186E-5 8 0.0 0.0 0.0 0.0 4.2187773561344186E-5 9 0.0 0.0 0.0 0.0 4.2187773561344186E-5 10-11 0.0 0.0 0.0 0.0 4.2187773561344186E-5 12-13 0.0 0.0 0.0 2.1093886780672093E-5 4.2187773561344186E-5 14-15 0.0 0.0 0.0 4.2187773561344186E-5 4.2187773561344186E-5 16-17 0.0 0.0 0.0 4.2187773561344186E-5 4.2187773561344186E-5 18-19 0.0 0.0 0.0 6.328166034201628E-5 4.2187773561344186E-5 20-21 0.0 0.0 0.0 8.437554712268837E-5 4.2187773561344186E-5 22-23 0.0 0.0 0.0 1.2656332068403255E-4 6.328166034201628E-5 24-25 0.0 0.0 0.0 1.4765720746470464E-4 8.437554712268837E-5 26-27 0.0 0.0 0.0 2.531266413680651E-4 8.437554712268837E-5 28-29 0.0 0.0 0.0 6.328166034201628E-4 8.437554712268837E-5 30-31 0.0 0.0 0.0 0.0016453231688924232 8.437554712268837E-5 32-33 0.0 0.0 0.0 0.003543772979152912 1.2656332068403255E-4 34-35 0.0 0.0 0.0 0.005695349430781465 1.2656332068403255E-4 36-37 0.0 0.0 0.0 0.008121146410558756 1.2656332068403255E-4 38-39 0.0 0.0 0.0 0.011137572220194864 1.2656332068403255E-4 40-41 0.0 0.0 0.0 0.015377443463109958 1.2656332068403255E-4 42-43 0.0 0.0 0.0 0.021009511233549404 1.2656332068403255E-4 44-45 0.0 0.0 0.0 0.027485334475215735 1.2656332068403255E-4 46-47 0.0 0.0 0.0 0.03455178654674089 1.2656332068403255E-4 48-49 0.0 0.0 0.0 0.041407299750459314 1.2656332068403255E-4 50-51 0.0 0.0 0.0 0.05159564706552394 1.2656332068403255E-4 52-53 0.0 0.0 0.0 0.07469345309035988 1.2656332068403255E-4 54-55 0.0 0.0 0.0 0.10627100160102601 1.2656332068403255E-4 56-57 0.0 0.0 0.0 0.13487431207561734 1.2656332068403255E-4 58-59 0.0 0.0 0.0 0.1767245834484708 1.2656332068403255E-4 60-61 0.0 0.0 0.0 0.22720225451461912 1.2656332068403255E-4 62-63 0.0 0.0 0.0 0.25854777027069786 1.2656332068403255E-4 64-65 0.0 0.0 0.0 0.28630732527406233 1.2656332068403255E-4 66-67 0.0 0.0 0.0 0.3614859377603777 1.2656332068403255E-4 68-69 0.0 0.0 0.0 0.44290834073377194 1.2656332068403255E-4 70-71 0.0 0.0 0.0 0.476996061771338 1.6875109424537675E-4 72-73 0.0 0.0 0.0 0.5411636653581425 2.1093886780672094E-4 74-75 0.0 0.0 0.0 0.6380478873417694 2.1093886780672094E-4 76-77 0.0 0.0 0.0 0.7314727118933662 2.1093886780672094E-4 78-79 0.0 0.0 0.0 0.8274920845189856 2.1093886780672094E-4 80-81 0.0 0.0 0.0 0.953549151920282 2.1093886780672094E-4 82-83 0.0 0.0 0.0 1.0956797610484506 2.1093886780672094E-4 84-85 0.0 0.0 0.0 1.2469018353790888 2.1093886780672094E-4 86-87 0.0 0.0 0.0 1.4711298518576332 2.1093886780672094E-4 88-89 0.0 0.0 0.0 1.7508769783429066 2.1093886780672094E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGTAT 65 0.005822951 29.253702 8 GTACATG 10310 0.0 26.876944 1 TACATGG 10800 0.0 25.347319 2 ACATGGG 10870 0.0 24.398632 3 GAGTACT 8275 0.0 21.970171 12-13 AATACCG 135 3.7791452E-4 21.126335 5 CATGGGA 5370 0.0 21.065989 4 GTACTTT 8700 0.0 20.785015 14-15 AGTACTT 8670 0.0 20.229132 12-13 GTACCTG 1720 0.0 19.89638 1 TACACCG 170 7.914442E-5 19.572927 5 TACCTGG 1680 0.0 19.5197 2 CATGGGG 4810 0.0 19.071789 4 GTATAGG 805 0.0 18.894007 1 GTGTAGC 1160 0.0 18.848198 1 ACTTTTT 10105 0.0 18.247023 16-17 GCTTATG 745 0.0 17.863714 1 TACTTTT 9625 0.0 17.775291 14-15 AGACCCG 680 0.0 17.475828 5 TAGCGCG 165 0.001457998 17.284088 4 >>END_MODULE