##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936424_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2370355 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.815424693769497 31.0 31.0 34.0 30.0 34.0 2 31.95526492867102 31.0 31.0 34.0 30.0 34.0 3 32.04323867100076 33.0 31.0 34.0 30.0 34.0 4 35.734122104073016 37.0 35.0 37.0 35.0 37.0 5 35.56670709661633 37.0 35.0 37.0 33.0 37.0 6 35.674408263741086 37.0 35.0 37.0 33.0 37.0 7 35.61923129657794 37.0 35.0 37.0 33.0 37.0 8 35.59551501779269 37.0 35.0 37.0 33.0 37.0 9 37.199535934490825 39.0 37.0 39.0 33.0 39.0 10-11 37.07820853838349 39.0 37.0 39.0 33.0 39.0 12-13 36.98440465668645 39.0 37.0 39.0 33.0 39.0 14-15 38.031175920906364 40.0 37.0 41.0 33.0 41.0 16-17 37.95668433631249 40.0 37.0 41.0 33.0 41.0 18-19 38.155256491116305 40.0 37.5 41.0 33.0 41.0 20-21 38.18112561198639 40.0 37.0 41.0 33.0 41.0 22-23 38.09544561890519 40.0 37.0 41.0 33.0 41.0 24-25 37.92978857597279 40.0 37.0 41.0 33.0 41.0 26-27 37.708764931835105 39.5 37.0 41.0 32.5 41.0 28-29 37.65004651202035 39.0 37.0 41.0 32.5 41.0 30-31 37.65178865612957 39.5 37.0 41.0 32.5 41.0 32-33 37.491034254362745 39.0 36.0 41.0 32.0 41.0 34-35 37.261471172039634 39.0 36.0 41.0 31.0 41.0 36-37 37.03703221669328 39.0 35.5 41.0 31.0 41.0 38-39 36.86197911283331 39.0 35.0 41.0 31.0 41.0 40-41 36.73364559317064 39.0 35.0 41.0 30.5 41.0 42-43 36.43595938161162 39.0 35.0 40.0 30.0 41.0 44-45 36.154134718217314 39.0 35.0 40.0 30.0 41.0 46-47 35.77219762440647 38.0 35.0 40.0 28.5 41.0 48-49 35.342857926344365 38.0 34.5 40.0 27.0 41.0 50-51 35.02960864511856 38.0 34.0 40.0 26.0 41.0 52-53 34.70882294002375 38.0 34.0 40.0 26.0 41.0 54-55 34.53525526767088 38.0 34.0 40.0 25.5 41.0 56-57 34.17652777748481 38.0 33.0 40.0 24.5 41.0 58-59 33.79510642920575 37.0 33.0 40.0 23.0 41.0 60-61 33.293882772833605 36.5 32.0 39.0 22.0 41.0 62-63 33.01020838650751 36.0 32.0 39.0 22.0 40.0 64-65 32.603965228837026 36.0 31.0 39.0 21.5 40.0 66-67 32.11949581391817 35.0 31.0 38.0 19.5 40.0 68-69 31.732441975990938 35.0 31.0 38.0 19.0 40.0 70-71 31.30613958668638 35.0 30.5 37.0 17.5 39.0 72-73 30.811367284647236 34.0 30.0 37.0 14.5 39.0 74-75 30.335280158457277 34.0 29.0 36.0 11.0 39.0 76-77 28.859800114328866 32.5 27.5 34.5 8.0 36.5 78-79 29.63406430682324 34.0 29.0 35.0 7.5 37.0 80-81 29.363736655479876 34.0 29.0 35.0 7.0 37.0 82-83 29.039459279306264 34.0 29.0 35.0 2.0 36.0 84-85 28.645296168717344 33.0 28.5 35.0 2.0 36.0 86-87 28.335210970508637 33.0 28.0 35.0 2.0 35.5 88-89 27.838270216908438 33.0 26.5 35.0 2.0 35.0 90-91 27.309768157090396 33.0 26.0 35.0 2.0 35.0 92-93 26.702549829034048 32.0 25.0 34.0 2.0 35.0 94-95 26.108574664976345 32.0 24.5 34.0 2.0 35.0 96-97 25.319244585726608 32.0 21.5 34.0 2.0 35.0 98-99 24.426783118984286 31.0 10.0 34.0 2.0 35.0 100-101 21.137331328007832 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.35753616489229856 1101 2 0.31571604434427414 1101 3 0.3580727453990846 1101 4 0.28834958314605785 1101 5 0.2552751422990056 1101 6 0.2584981165172451 1101 7 0.3030931823052043 1101 8 0.30086922057948584 1101 9 0.28135485705593766 1101 10-11 0.4260899318709477 1101 12-13 0.34216186862150266 1101 14-15 0.5807729661312493 1101 16-17 0.6089597010520791 1101 18-19 0.4576019251828356 1101 20-21 0.4698153639540834 1101 22-23 0.42493924493504664 1101 24-25 0.371297090855812 1101 26-27 0.5163926077722181 1101 28-29 0.37380828176697634 1101 30-31 0.4716554577853742 1101 32-33 0.5750336432895793 1101 34-35 0.5648631168672793 1101 36-37 0.5627730964067297 1101 38-39 0.4816739337593816 1101 40-41 0.6332421447284275 1101 42-43 0.5666187955573321 1101 44-45 0.5435987408581795 1101 46-47 0.4032098270558606 1101 48-49 0.4526355995856832 1101 50-51 0.48476806408145734 1101 52-53 0.6714573145579479 1101 54-55 0.5038096282873212 1101 56-57 0.5307528861136177 1101 58-59 0.5228513886483555 1101 60-61 0.6392524253564815 1101 62-63 0.7208228238809156 1101 64-65 0.678404046247536 1101 66-67 0.4068406872074277 1101 68-69 0.5734663950909002 1101 70-71 0.5476011781168388 1101 72-73 0.5577818786470559 1101 74-75 0.6319190850493541 1101 76-77 0.47747697766765285 1101 78-79 0.6175915460787422 1101 80-81 0.5792719385999483 1101 82-83 0.5276019469755688 1101 84-85 0.6041259818668223 1101 86-87 0.5839748502590538 1101 88-89 0.5826528317109343 1101 90-91 0.7261768306845582 1101 92-93 0.6862750318711868 1101 94-95 0.7306528244348485 1101 96-97 0.6361871256054101 1101 98-99 0.5040578368491531 1101 100-101 1.199495262095649 1102 1 0.24600163438647726 1102 2 0.19768161762219805 1102 3 0.2728468370684993 1102 4 0.20418070925367715 1102 5 0.20202542558320857 1102 6 0.2236343200410218 1102 7 0.20279214945554713 1102 8 0.21596126244881475 1102 9 0.37760858097266947 1102 10-11 0.34029299869344953 1102 12-13 0.4249322758394385 1102 14-15 0.5269585664323628 1102 16-17 0.4623205546463822 1102 18-19 0.36704062395578774 1102 20-21 0.35039952502919647 1102 22-23 0.3328152741809802 1102 24-25 0.25746132078985795 1102 26-27 0.311704924854439 1102 28-29 0.3819417221235426 1102 30-31 0.4309759150783208 1102 32-33 0.46116762081915397 1102 34-35 0.42683279350249137 1102 36-37 0.40408629415101416 1102 38-39 0.3928916967371876 1102 40-41 0.5306244461633867 1102 42-43 0.4952712849017118 1102 44-45 0.5399625389627118 1102 46-47 0.3916196424099212 1102 48-49 0.6119184513401663 1102 50-51 0.506679803505584 1102 52-53 0.68730751767756 1102 54-55 0.7110921619201989 1102 56-57 0.5396546347025932 1102 58-59 0.7147945820030444 1102 60-61 0.6926225517841118 1102 62-63 0.7230069103620025 1102 64-65 0.6313435601213868 1102 66-67 0.5415772693233336 1102 68-69 0.5823122667175262 1102 70-71 0.6418360468154027 1102 72-73 0.6798115081776501 1102 74-75 0.4318456222700071 1102 76-77 0.414910634487466 1102 78-79 0.6331375968373045 1102 80-81 0.739162923674801 1102 82-83 0.7089736818369836 1102 84-85 0.4781034634509922 1102 86-87 0.6683704029327728 1102 88-89 0.5840941488166393 1102 90-91 0.45030146921974534 1102 92-93 0.5351940097615611 1102 94-95 0.48975858392703486 1102 96-97 0.6553926888940396 1102 98-99 0.6524923306235877 1102 100-101 1.4860421806611477 1103 1 0.17119244937601152 1103 2 0.2045736594342067 1103 3 0.21125203924886193 1103 4 0.15053738871828415 1103 5 0.27494133372548646 1103 6 0.12752029698949485 1103 7 0.12925323995066407 1103 8 0.07782724909688454 1103 9 0.16469441475933166 1103 10-11 0.12852584033640113 1103 12-13 0.16907813335630806 1103 14-15 0.2776154782969442 1103 16-17 0.1589272199518703 1103 18-19 0.17311895650144749 1103 20-21 0.21518276672088632 1103 22-23 0.06156623973790687 1103 24-25 0.08447397452341221 1103 26-27 0.13896006842317377 1103 28-29 0.10043577219876454 1103 30-31 0.104724284399623 1103 32-33 0.06309545070408262 1103 34-35 0.033658965651717665 1103 36-37 -0.00876895107784037 1103 38-39 0.10228440605065714 1103 40-41 0.1030594200987025 1103 42-43 0.26588185003390663 1103 44-45 0.15887274823691655 1103 46-47 0.0852041085919879 1103 48-49 -0.0692100330531531 1103 50-51 0.06729774089989604 1103 52-53 0.09478277252986089 1103 54-55 -0.014502866252890101 1103 56-57 -0.1253605310257626 1103 58-59 0.13424631224773975 1103 60-61 0.05952180879906166 1103 62-63 0.21992331538237408 1103 64-65 0.2556437544464174 1103 66-67 0.11311771991180564 1103 68-69 0.17933033185328995 1103 70-71 0.1291128264742447 1103 72-73 0.18392027117489107 1103 74-75 0.13607822404078007 1103 76-77 0.199182491531257 1103 78-79 0.2309461931138621 1103 80-81 0.2653070878082957 1103 82-83 0.30872624225835565 1103 84-85 0.6250099451876281 1103 86-87 0.5799056372531552 1103 88-89 0.34824172922498065 1103 90-91 0.4605260209943012 1103 92-93 0.41439504501944313 1103 94-95 0.2478841351214207 1103 96-97 0.682767625772911 1103 98-99 0.6440156570034823 1103 100-101 1.0921004286708964 1104 1 0.21523759144668375 1104 2 0.28351568482617395 1104 3 0.18886741888857017 1104 4 0.1908340176168295 1104 5 0.24656672856588102 1104 6 0.12590365242311208 1104 7 0.1523129969553736 1104 8 0.1681217218704134 1104 9 0.2427663538703584 1104 10-11 0.26226443533347776 1104 12-13 0.26778688398967176 1104 14-15 0.3017568913494131 1104 16-17 0.4464143730403407 1104 18-19 0.3086428679278299 1104 20-21 0.21756708163012917 1104 22-23 0.2226287781438785 1104 24-25 0.3065384552386732 1104 26-27 0.28612930087860633 1104 28-29 0.3275368261845415 1104 30-31 0.3748842778593726 1104 32-33 0.46317949466028807 1104 34-35 0.38904519052837117 1104 36-37 0.3960457659127812 1104 38-39 0.2972358393347534 1104 40-41 0.4834135726158024 1104 42-43 0.4517966489572842 1104 44-45 0.6187607375425372 1104 46-47 0.6018210945448317 1104 48-49 0.6332824474608643 1104 50-51 0.5783353041911496 1104 52-53 0.5468249373512393 1104 54-55 0.6069628854980706 1104 56-57 0.45875956334063517 1104 58-59 0.5880764886546999 1104 60-61 0.7405828558852434 1104 62-63 0.6417658050599186 1104 64-65 0.5695955452879176 1104 66-67 0.6239071042303124 1104 68-69 0.755602580368258 1104 70-71 0.8180232915863392 1104 72-73 0.822818742841612 1104 74-75 0.590565120191016 1104 76-77 0.67515741939091 1104 78-79 0.6014515708489974 1104 80-81 0.9774295862540896 1104 82-83 0.844168445530638 1104 84-85 1.0575535528159996 1104 86-87 1.1311041732442675 1104 88-89 1.017874864554166 1104 90-91 1.088654199032728 1104 92-93 1.15581726101286 1104 94-95 1.268281302332511 1104 96-97 1.4066558288942623 1104 98-99 1.3744928434040844 1104 100-101 1.9335160864507337 1105 1 0.05403460181638309 1105 2 0.12273282181414302 1105 3 0.11996099570965413 1105 4 0.1628147927620489 1105 5 0.2907740297165873 1105 6 0.18608606513368642 1105 7 0.17970710937986212 1105 8 0.23164338682524033 1105 9 0.16623875259605114 1105 10-11 0.22932119884977453 1105 12-13 0.2575496343102799 1105 14-15 0.3343584848130732 1105 16-17 0.33261743520630915 1105 18-19 0.3609205605970871 1105 20-21 0.27133748591496243 1105 22-23 0.2904560155792524 1105 24-25 0.2501075150022629 1105 26-27 0.20962701455419364 1105 28-29 0.2175129764552537 1105 30-31 0.2802585184108821 1105 32-33 0.1981234826135605 1105 34-35 0.2148228454790697 1105 36-37 0.2693107126135672 1105 38-39 0.30700668463648384 1105 40-41 0.3292545947304717 1105 42-43 0.33857216265491985 1105 44-45 0.38361894480886605 1105 46-47 0.3450429260415646 1105 48-49 0.27722571876169155 1105 50-51 0.3108939565363684 1105 52-53 0.4840329087315709 1105 54-55 0.41892300248360215 1105 56-57 0.2426541051763138 1105 58-59 0.18984113650851953 1105 60-61 0.45615002681990546 1105 62-63 0.3601044915259237 1105 64-65 0.41812938172867575 1105 66-67 0.29931062017944754 1105 68-69 0.3020604458464966 1105 70-71 0.30861983946859084 1105 72-73 0.4490682423454686 1105 74-75 0.3181353492296566 1105 76-77 0.16919745327957614 1105 78-79 0.12913510060893074 1105 80-81 0.1825073009741871 1105 82-83 0.4489526083819264 1105 84-85 0.5964402898132555 1105 86-87 0.8599526259132944 1105 88-89 0.6913418956400577 1105 90-91 0.6096000351949513 1105 92-93 0.6214217152834713 1105 94-95 0.7697174361231092 1105 96-97 0.7996930571030951 1105 98-99 0.7055717680192082 1105 100-101 1.1199053476894143 1106 1 0.0693537290965125 1106 2 0.11856261090441578 1106 3 0.011259242854265494 1106 4 0.09414081223175685 1106 5 0.1369495188221279 1106 6 -0.016675172843150676 1106 7 0.09740069775329374 1106 8 0.04192132818346295 1106 9 0.2698666034397661 1106 10-11 0.12803036735218143 1106 12-13 0.17547696096953302 1106 14-15 0.2684551935744466 1106 16-17 0.11441456636875103 1106 18-19 0.2256017241266406 1106 20-21 0.14973868695896186 1106 22-23 0.046951249935510475 1106 24-25 -0.02469418914250099 1106 26-27 0.05778691626051824 1106 28-29 -0.11787601785215429 1106 30-31 -0.06313683008817605 1106 32-33 -0.14282906451578725 1106 34-35 -0.01364778637767472 1106 36-37 -0.11270234474137197 1106 38-39 0.19475596306494225 1106 40-41 0.19694963715234337 1106 42-43 0.1685859034126267 1106 44-45 -0.009166430371983836 1106 46-47 0.103447689504236 1106 48-49 0.0435921244266666 1106 50-51 0.14852582055376473 1106 52-53 -0.10889714426850361 1106 54-55 0.020925590689820694 1106 56-57 -0.27326045493768447 1106 58-59 -0.04072768723900566 1106 60-61 -0.022133759813023346 1106 62-63 -0.12930567372667667 1106 64-65 -0.05391362902401653 1106 66-67 0.051883922396150695 1106 68-69 -0.2028164326023223 1106 70-71 -0.009226787502552014 1106 72-73 0.19825724683121138 1106 74-75 0.0053688039760366735 1106 76-77 -0.03548530970325814 1106 78-79 -0.07405108302284447 1106 80-81 -0.030198537757300414 1106 82-83 0.11345383341773285 1106 84-85 0.4457205358382481 1106 86-87 0.5325743479428553 1106 88-89 0.46737045315130743 1106 90-91 0.4597138144849424 1106 92-93 0.5024656493569104 1106 94-95 0.3526792600814659 1106 96-97 0.6374499692858393 1106 98-99 0.6542900243243288 1106 100-101 0.9855363683587122 1107 1 0.04533174885856184 1107 2 0.06880459517543969 1107 3 0.08621639147293791 1107 4 0.10362105018295154 1107 5 0.06309390277050397 1107 6 -0.03176642107868588 1107 7 0.0018824481061869847 1107 8 -0.010730416119820063 1107 9 -0.04876618542895983 1107 10-11 0.042241901935760495 1107 12-13 0.00484023985640647 1107 14-15 0.062483727553065194 1107 16-17 0.151052497403235 1107 18-19 -0.017655484988097214 1107 20-21 -0.02909147033081183 1107 22-23 -0.03710551554140551 1107 24-25 0.04917150958708305 1107 26-27 0.050008130214941104 1107 28-29 0.09162690612446056 1107 30-31 0.10302822335001594 1107 32-33 0.04729747309260546 1107 34-35 0.1401685072299088 1107 36-37 0.12417126889041441 1107 38-39 0.032597513780814324 1107 40-41 0.041314680507852586 1107 42-43 0.13640955737511717 1107 44-45 0.08341649965424125 1107 46-47 0.17824351527556104 1107 48-49 0.2339165345900085 1107 50-51 0.17338466306232903 1107 52-53 0.2071658220553516 1107 54-55 0.13241858322863465 1107 56-57 0.05906839160395805 1107 58-59 0.01390908428751203 1107 60-61 0.25440132790627246 1107 62-63 0.17189416495047283 1107 64-65 0.14970479202862208 1107 66-67 0.33355991553992936 1107 68-69 0.4390720529794443 1107 70-71 0.37977553151337773 1107 72-73 0.3436923143851516 1107 74-75 0.5067740772166083 1107 76-77 0.20514662213783552 1107 78-79 0.40544957235122325 1107 80-81 0.5454807826702037 1107 82-83 0.5041840713689325 1107 84-85 0.6659904995406087 1107 86-87 0.6745094659101838 1107 88-89 0.46189177801518966 1107 90-91 0.3942784948640501 1107 92-93 0.6303984483083163 1107 94-95 0.6631740675023146 1107 96-97 0.9624108989087503 1107 98-99 0.9576752834491522 1107 100-101 1.5912431640836537 1108 1 0.0478376520662529 1108 2 0.11677619211998191 1108 3 0.08803062705546694 1108 4 0.12626960578587187 1108 5 0.19056202995954408 1108 6 0.15161345569256213 1108 7 0.18815402272340975 1108 8 0.1722475971096742 1108 9 0.1476916644277182 1108 10-11 0.20971455097061664 1108 12-13 0.1981850920563204 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-0.9433115296190344 2316 82-83 -1.1663256503197168 2316 84-85 -0.9822038915466003 2316 86-87 -1.2178956649217625 2316 88-89 -1.2310760048848834 2316 90-91 -1.3069956759644796 2316 92-93 -1.4992182866556156 2316 94-95 -1.341195890238751 2316 96-97 -1.6259606915637832 2316 98-99 -2.1963897376803168 2316 100-101 -3.243816275878782 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 5.0 4 27.0 5 70.0 6 180.0 7 452.0 8 1015.0 9 1798.0 10 3112.0 11 4870.0 12 6822.0 13 9720.0 14 12516.0 15 16357.0 16 20939.0 17 26371.0 18 23714.0 19 16867.0 20 15745.0 21 16312.0 22 17638.0 23 19663.0 24 23114.0 25 27609.0 26 33698.0 27 41695.0 28 51723.0 29 63593.0 30 77437.0 31 95185.0 32 117590.0 33 147654.0 34 190176.0 35 255129.0 36 351658.0 37 428526.0 38 238122.0 39 13249.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.435939491809854 19.041876295698355 14.695587631510879 21.826596580980908 2 15.60529575772305 21.927404001944264 37.636199773169146 24.831100467163534 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22.884378078389105 22-23 21.83203630347651 25.765292615884185 29.3142810254091 23.088390055230203 24-25 23.507364930569473 25.8245283934263 29.038582828310528 21.629523847693697 26-27 22.65956607528354 24.989695479806727 30.85911643116391 21.49162201374583 28-29 21.083108659557688 28.044033458374372 29.543239145783307 21.329618736284633 30-31 21.81073787052471 25.836395154917835 29.1865321194893 23.16633485506815 32-33 21.95603754853577 26.195078051725645 29.648854781472465 22.200029618266115 34-35 23.70781506361834 25.551099498925666 29.045921055795727 21.695164381660266 36-37 22.92671844706888 24.985882770356284 30.339376724047558 21.74802205852728 38-39 21.995446197929652 26.354718146483584 30.210156657710098 21.439678997876666 40-41 22.152539562739495 26.73718089614652 29.293007063516807 21.81727247759718 42-43 21.549839224226037 27.071273568158993 27.953362263057986 23.425524944556987 44-45 22.538392641303187 25.616701774577262 28.26251491892836 23.58239066519119 46-47 23.77306647265879 26.940551618842868 27.867629702864615 21.418752205633723 48-49 23.392750994688946 26.583180718032317 28.023438841448417 22.00062944583032 50-51 21.453691266107313 27.137045468352376 29.442378178430882 21.966885087109425 52-53 22.953588509378676 28.05964771876564 26.962636960230142 22.02412681162554 54-55 21.7308248059857 26.500612787751837 28.016571637103088 23.751990769159374 56-57 23.14028088666854 26.385351023947308 27.601513927244568 22.872854162139582 58-59 24.90626980076174 25.902471641458487 26.984886908704286 22.206371649075486 60-61 22.828295189016124 26.72550333092459 29.280066671238007 21.16613480882128 62-63 22.164634718787823 26.908800002025302 28.79133963835273 22.135225640834147 64-65 21.63257314750857 28.478697229380273 27.634337776693812 22.25439184641734 66-67 22.46152995883197 26.919620670248236 26.81929522428939 23.799554146630406 68-69 21.95907370879511 26.97026516721585 27.681599369202953 23.389061754786088 70-71 23.476260066015513 26.85160263117039 27.052922941402795 22.619214361411302 72-73 23.040156432264368 25.693893952593598 28.950093973265606 22.31585564187643 74-75 21.855650618072772 28.82808862885743 27.190687806222936 22.125572946846862 76-77 22.46365958667592 26.726356845877834 27.109937792553247 23.700045774893 78-79 22.76842334432294 26.969986304479356 27.6135618564139 22.648028494783805 80-81 23.74961681575953 26.860260592710333 27.022838308170638 22.3672842833595 82-83 23.045735635498932 26.462316029677872 28.21436309806244 22.27758523676075 84-85 22.395638485328227 27.472621844161267 27.821873870475017 22.309865800035492 86-87 22.427733421544175 27.892509068263127 27.03753756731316 22.642219942879542 88-89 21.994679172952626 27.931535343700233 26.091171550562947 23.9826139327842 90-91 22.621161541828446 26.527437592145574 26.809240030339186 24.042160835686786 92-93 23.581458561171754 27.311682115896335 27.156911999632012 21.9499473232999 94-95 22.871903036779003 26.770557640647713 27.51680188993963 22.84073743263366 96-97 21.270152482456602 26.952799029177438 28.91601329604812 22.86103519231784 98-99 22.180968131637133 27.835705162136176 26.534576190198806 23.44875051602789 100-101 21.42674417672022 26.66069905107736 27.288649527607152 24.62390724459527 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 385.0 1 292.0 2 272.0 3 358.0 4 303.5 5 276.5 6 506.0 7 1056.0 8 1710.0 9 2141.5 10 2094.0 11 1902.5 12 2010.5 13 2251.5 14 2546.0 15 2873.0 16 3125.0 17 3353.0 18 3692.5 19 4111.0 20 4505.0 21 5066.5 22 5896.5 23 6922.0 24 8478.0 25 10320.0 26 12760.5 27 16035.5 28 19024.5 29 22084.0 30 25888.0 31 30385.0 32 36117.0 33 40274.5 34 43194.5 35 46227.0 36 50501.5 37 55220.0 38 57454.0 39 58329.0 40 59081.0 41 61524.5 42 64316.0 43 67352.5 44 70181.5 45 73645.5 46 78138.0 47 108368.5 48 172966.5 49 167545.5 50 106143.5 51 81122.0 52 73491.5 53 71590.5 54 70799.5 55 66272.0 56 60434.5 57 54165.0 58 48890.0 59 43801.0 60 37102.0 61 30745.5 62 26005.5 63 20810.0 64 15530.0 65 12204.0 66 9124.5 67 7080.0 68 5287.5 69 3554.0 70 2667.0 71 2160.5 72 1901.5 73 1659.0 74 1367.5 75 1165.0 76 860.5 77 490.5 78 328.5 79 247.5 80 164.5 81 118.5 82 77.0 83 52.5 84 34.5 85 18.0 86 10.5 87 5.0 88 1.0 89 0.5 90 2.0 91 1.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02084076013930403 2 0.012951646483332666 3 0.009492249051302441 4 0.0 5 0.00312189524353947 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 9.070371315689001E-4 14-15 2.3203275458739305E-4 16-17 2.1093886780672093E-5 18-19 0.0014765720746470464 20-21 0.0 22-23 1.4765720746470464E-4 24-25 0.0 26-27 0.0015187598482083908 28-29 0.0028054869418293886 30-31 0.0014343843010857023 32-33 0.00826880361802346 34-35 0.0020250131309445213 36-37 0.0010336004522529326 38-39 0.001181257659717637 40-41 2.5312664136806516E-4 42-43 0.005421128902632728 44-45 0.0018562620366991444 46-47 1.8984498102604886E-4 48-49 6.75004376981507E-4 50-51 1.2656332068403258E-4 52-53 1.8984498102604886E-4 54-55 0.0016875109424537675 56-57 0.015187598482083908 58-59 0.02761189779589977 60-61 0.021832172817995616 62-63 0.014280561350515008 64-65 0.019068873649727574 66-67 0.002910956375732749 68-69 8.648493580075558E-4 70-71 1.2656332068403258E-4 72-73 0.0 74-75 0.0018351681499184723 76-77 0.0025312664136806513 78-79 0.009998502334038572 80-81 0.017360268820493133 82-83 0.018625902027333458 84-85 0.030354103077387142 86-87 0.011643825502930995 88-89 0.041618238618266044 90-91 0.04817843740705506 92-93 0.030016600888896388 94-95 0.03187286292559553 96-97 0.05189096148045335 98-99 0.054717542309063415 100-101 0.061509773852439824 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2370355.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.42815704851809 #Duplication Level Percentage of deduplicated Percentage of total 1 74.87536866709434 34.76325375541546 2 14.121395595869549 13.11260744938566 3 4.7535256492181395 6.62092306128176 4 2.069502196509386 3.8433269196716364 5 1.0942464655454835 2.540192337606577 6 0.6665329672205881 1.8567538368079333 7 0.4411822369421744 1.4338294728637455 8 0.3006480503240764 1.1166827917421591 9 0.23083250894822518 0.9645415179616489 >10 1.2401561455768115 10.783321252757181 >50 0.12174776072620883 3.9551768582208844 >100 0.07422390839584318 6.317538673451682 >500 0.006455445997219427 2.018935705073581 >1k 0.002818575012870438 2.8080712157818617 >5k 7.273741968697905E-4 2.6570320129727114 >10k+ 6.364524222610666E-4 5.207813139005615 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 41882 1.7669083322962174 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 20676 0.8722744061543525 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 14568 0.6145914852416622 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 14262 0.6016820265318908 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 11153 0.4705202385296717 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 10633 0.4485825962777727 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 10195 0.430104351457904 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 9444 0.39842133351333453 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9171 0.38690407133108756 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8942 0.37724307118553974 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 8598 0.3627304770804373 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 8234 0.34737412750410807 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 7117 0.30025038443608654 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 6426 0.27109863290519776 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5011 0.21140293331589574 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4934 0.2081544747516722 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4002 0.16883546979249942 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3673 0.1549556922908172 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3664 0.15457600232876512 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3656 0.15423850014027435 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3609 0.15225567478289118 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3391 0.14305874014651812 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2862 0.12074140793256706 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2629 0.11091165669277388 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2604 0.10985696235374026 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2470 0.10420380069652013 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2389 0.10078659103805127 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 4.2187773561344186E-5 16-17 0.0 0.0 0.0 0.0 4.2187773561344186E-5 18-19 0.0 0.0 0.0 2.1093886780672093E-5 4.2187773561344186E-5 20-21 0.0 0.0 0.0 4.2187773561344186E-5 4.2187773561344186E-5 22-23 0.0 0.0 0.0 8.437554712268837E-5 6.328166034201628E-5 24-25 0.0 0.0 0.0 1.0546943390336047E-4 8.437554712268837E-5 26-27 0.0 0.0 0.0 2.3203275458739302E-4 8.437554712268837E-5 28-29 0.0 0.0 0.0 6.328166034201628E-4 8.437554712268837E-5 30-31 0.0 0.0 0.0 0.001560947621769735 8.437554712268837E-5 32-33 0.0 0.0 0.0 0.0035648668659335834 8.437554712268837E-5 34-35 0.0 0.0 0.0 0.005821912751465498 8.437554712268837E-5 36-37 0.0 0.0 0.0 0.008332085278365476 8.437554712268837E-5 38-39 0.0 0.0 0.0 0.011411792748343603 8.437554712268837E-5 40-41 0.0 0.0 0.0 0.01582041508550407 8.437554712268837E-5 42-43 0.0 0.0 0.0 0.02164232783696957 8.437554712268837E-5 44-45 0.0 0.0 0.0 0.02801268164473254 8.437554712268837E-5 46-47 0.0 0.0 0.0 0.035142415376599706 1.2656332068403255E-4 48-49 0.0 0.0 0.0 0.04206121024066015 1.2656332068403255E-4 50-51 0.0 0.0 0.0 0.05201752480113739 1.2656332068403255E-4 52-53 0.0 0.0 0.0 0.07509423693919265 1.2656332068403255E-4 54-55 0.0 0.0 0.0 0.10681944265732349 1.2656332068403255E-4 56-57 0.0 0.0 0.0 0.13519072037732743 1.4765720746470464E-4 58-59 0.0 0.0 0.0 0.17683005288237416 1.6875109424537675E-4 60-61 0.0 0.0 0.0 0.22680147066578635 1.6875109424537675E-4 62-63 0.0 0.0 0.0 0.25825245585576845 1.6875109424537675E-4 64-65 0.0 0.0 0.0 0.2858221658781069 1.6875109424537675E-4 66-67 0.0 0.0 0.0 0.3609796844776415 1.8984498102604886E-4 68-69 0.0 0.0 0.0 0.4428028712998686 2.1093886780672094E-4 70-71 0.0 0.0 0.0 0.4767218412431893 2.1093886780672094E-4 72-73 0.0 0.0 0.0 0.5405730365282837 2.1093886780672094E-4 74-75 0.0 0.0 0.0 0.6370142868895166 2.1093886780672094E-4 76-77 0.0 0.0 0.0 0.7294055109888603 2.1093886780672094E-4 78-79 0.0 0.0 0.0 0.8251084753127695 2.1093886780672094E-4 80-81 0.0 0.0 0.0 0.9506381955445492 2.1093886780672094E-4 82-83 0.0 0.0 0.0 1.092494584144569 2.1093886780672094E-4 84-85 0.0 0.0 0.0 1.2424932130419282 2.1093886780672094E-4 86-87 0.0 0.0 0.0 1.4644430897481602 2.1093886780672094E-4 88-89 0.0 0.0 0.0 1.742080827555366 2.1093886780672094E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9950 0.0 22.681679 1 GAGTACT 10430 0.0 21.925146 12-13 GTACACG 310 1.0913936E-11 21.457098 1 TACATGG 10430 0.0 21.449743 2 ACATGGG 10000 0.0 20.944016 3 GTAAGCG 330 0.0 20.88316 94-95 GTCTACG 185 6.5424465E-6 20.545794 1 GTACTTT 11125 0.0 20.38382 14-15 CATGGGG 4445 0.0 19.658443 4 AGTACTT 10825 0.0 19.457912 12-13 ACTTTTT 12905 0.0 17.75626 16-17 TAAGGTG 1035 0.0 17.435978 5 TACTTTT 12360 0.0 16.983063 14-15 ATTCGCG 280 0.0 16.965939 80-81 AATTCGC 295 0.0 16.908426 78-79 GTACCTG 1760 0.0 16.737234 1 AGAGTAC 18745 0.0 16.531311 10-11 CTCGCGC 275 0.0 16.411901 60-61 TACCTGG 1695 0.0 16.253384 2 GTATAGG 800 0.0 16.035349 1 >>END_MODULE