FastQCFastQC Report
Thu 26 May 2016
SRR936423_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936423_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1532006
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97490.6363552100970884No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73370.4789145734416184No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45680.2981711559876397No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31290.20424201993986968No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT29370.19170943194739445No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA28130.18361546886892088No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA22150.1445816791840241No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT20140.13146162612940157No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC19560.12767574017334135No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19160.1250647843415757No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT17820.11631808230516069No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG95600.025.094661
ATGGGCG2101.2623786E-924.8889315
GAGTACT75800.024.09766412-13
TACATGG97900.024.0683752
GTATCAA152100.023.8310491
ACATGGG95650.023.2965933
GTACTTT82200.022.36921514-15
AGTACTT78750.022.01862112-13
GGTATCA114450.021.5011541
CCGTCTA1353.7893633E-421.1171919
ATCAACG173250.020.8422623
TCAACGC174500.020.6119544
TATCAAC175850.020.5044062
CATTCCG2551.1887096E-820.49619
CAACGCA175700.020.4448015
AACGCAG178750.020.2022846
CATGGGG37450.020.1726974
GGATCGT1456.1340537E-419.6614786
ACTTTTT95950.019.38803916-17
CATGGGA52900.019.3108544
Produced by FastQC (version 0.11.5)