##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936423_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1532006 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11822864923506 33.0 31.0 34.0 31.0 34.0 2 32.242052576817585 33.0 31.0 34.0 31.0 34.0 3 32.26636645026194 34.0 31.0 34.0 31.0 34.0 4 35.88070803900246 37.0 35.0 37.0 35.0 37.0 5 35.90778952562849 37.0 35.0 37.0 35.0 37.0 6 35.93080118485176 37.0 36.0 37.0 35.0 37.0 7 35.88835226493891 37.0 35.0 37.0 35.0 37.0 8 35.86880469136544 37.0 35.0 37.0 35.0 37.0 9 37.502749989229805 39.0 37.0 39.0 35.0 39.0 10-11 37.40541420856054 39.0 37.0 39.0 35.0 39.0 12-13 37.33768307695922 39.0 37.0 39.0 35.0 39.0 14-15 38.56230328079655 40.0 38.0 41.0 34.5 41.0 16-17 38.477431224159695 40.0 38.0 41.0 34.0 41.0 18-19 38.47663879906476 40.0 38.0 41.0 34.0 41.0 20-21 38.37016858941806 40.0 38.0 41.0 34.0 41.0 22-23 38.49269650379959 40.0 38.0 41.0 34.0 41.0 24-25 38.53072931829249 40.0 38.0 41.0 34.0 41.0 26-27 38.443309295133304 40.0 38.0 41.0 34.0 41.0 28-29 38.312349951632044 40.0 38.0 41.0 34.0 41.0 30-31 38.092364520765585 40.0 38.0 41.0 34.0 41.0 32-33 37.88861825606427 40.0 37.0 41.0 33.5 41.0 34-35 37.70715160384489 40.0 37.0 41.0 33.0 41.0 36-37 37.51139878042253 40.0 37.0 41.0 33.0 41.0 38-39 37.31294590230064 39.5 36.5 41.0 33.0 41.0 40-41 37.10194248586494 39.0 36.0 41.0 32.0 41.0 42-43 36.848169654688036 39.0 36.0 41.0 31.0 41.0 44-45 36.5512556739334 39.0 35.5 40.5 31.0 41.0 46-47 36.25585049928003 39.0 36.0 41.0 30.0 41.0 48-49 36.0310938730005 39.0 36.0 41.0 29.5 41.0 50-51 35.06786461671821 38.0 34.0 40.0 28.0 40.5 52-53 35.16678100477413 38.5 35.0 39.5 28.0 40.5 54-55 35.59190531890867 39.0 35.0 40.0 28.0 41.0 56-57 35.492542131036046 39.0 35.0 41.0 28.0 41.0 58-59 35.27558247160911 38.5 35.0 40.0 28.0 41.0 60-61 35.00891249773173 38.0 35.0 40.0 27.0 41.0 62-63 34.66757049254376 38.0 34.0 40.0 26.0 41.0 64-65 34.31993706290967 37.0 34.0 40.0 26.0 41.0 66-67 33.8882315082317 37.0 34.0 39.5 25.5 41.0 68-69 33.51317488312709 36.0 33.5 39.0 24.5 41.0 70-71 33.11484452410761 36.0 33.0 39.0 23.5 40.0 72-73 32.7300043211319 35.0 33.0 38.0 22.5 40.0 74-75 32.30475402837848 35.0 32.5 37.0 21.5 39.5 76-77 31.85265462406805 35.0 32.0 37.0 20.0 39.0 78-79 31.38976609752181 35.0 31.5 36.0 18.5 39.0 80-81 30.952016832832246 35.0 31.0 36.0 17.0 37.5 82-83 30.560060469737063 34.5 31.0 35.5 12.0 37.0 84-85 30.205202198947 34.0 31.0 35.0 7.5 37.0 86-87 29.86926324048339 34.0 30.0 35.0 4.5 36.0 88-89 29.488726545457393 34.0 30.0 35.0 2.0 36.0 90-91 29.117598756140644 34.0 29.5 35.0 2.0 35.5 92-93 28.721512513658563 34.0 29.0 35.0 2.0 35.0 94-95 28.28002795028218 34.0 29.0 35.0 2.0 35.0 96-97 27.759709491999377 34.0 28.0 35.0 2.0 35.0 98-99 27.077280702555996 34.0 27.0 35.0 2.0 35.0 100-101 24.06450301108481 30.0 13.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3171415683952361 1101 2 0.2558883962306737 1101 3 0.25584529318214777 1101 4 0.24194978453629545 1101 5 0.05840261321974083 1101 6 0.16868047980138812 1101 7 0.220342288051242 1101 8 0.20711891310330088 1101 9 0.30372663952730505 1101 10-11 0.3025990626477508 1101 12-13 0.2973354686213199 1101 14-15 0.32287643221339124 1101 16-17 0.3381378912700441 1101 18-19 0.3292195792476136 1101 20-21 0.355141743204598 1101 22-23 0.24157647016497208 1101 24-25 0.1740131629046644 1101 26-27 0.16559261440946216 1101 28-29 0.15272343982709913 1101 30-31 0.19223786534667653 1101 32-33 0.1358072773751431 1101 34-35 0.04370910844684062 1101 36-37 0.06066562147520216 1101 38-39 0.014611590827463772 1101 40-41 -0.04841292993659607 1101 42-43 0.11572391137075755 1101 44-45 0.14725292581000105 1101 46-47 0.14356567009551213 1101 48-49 0.2820299638570205 1101 50-51 0.06054061204894623 1101 52-53 0.18964616836365877 1101 54-55 0.14204353019690785 1101 56-57 -0.022004885146039044 1101 58-59 0.039238806182780195 1101 60-61 0.07275756989123039 1101 62-63 -0.12837102934913247 1101 64-65 0.02018121956428587 1101 66-67 0.038075377673749244 1101 68-69 0.10942994361452207 1101 70-71 0.05790990490056913 1101 72-73 -0.1740147151863738 1101 74-75 -0.1288850918208837 1101 76-77 0.013542714454221283 1101 78-79 -0.06536039770938729 1101 80-81 -0.24503531231354359 1101 82-83 -0.2887149218012368 1101 84-85 -0.34446152599847224 1101 86-87 -0.1330400304438193 1101 88-89 -0.0193155661348392 1101 90-91 0.08419111090811171 1101 92-93 -0.09113782169123752 1101 94-95 -0.18388492124720202 1101 96-97 -0.3828491571099022 1101 98-99 -0.4413016257791753 1101 100-101 -0.13265670887805214 1102 1 0.22163893582870742 1102 2 0.20665878352459544 1102 3 0.16529555013502062 1102 4 0.12848843527893195 1102 5 0.18340872553432774 1102 6 0.2308163552291802 1102 7 0.22132968460583413 1102 8 0.2970235492129092 1102 9 0.10586055901442393 1102 10-11 0.28236140996508396 1102 12-13 0.3317940471591072 1102 14-15 0.3374272757717165 1102 16-17 0.20305514967749616 1102 18-19 0.21992447198606158 1102 20-21 0.1536763194635995 1102 22-23 0.13411505728062423 1102 24-25 0.10645603264666903 1102 26-27 -0.06339000776466008 1102 28-29 -0.13644103180674705 1102 30-31 0.18609756666510435 1102 32-33 0.03531419769353761 1102 34-35 0.07466923376843937 1102 36-37 -0.1757921405162719 1102 38-39 -0.28615685230856513 1102 40-41 -0.1976227770374166 1102 42-43 -0.25770139437752704 1102 44-45 -0.2974088290104788 1102 46-47 -0.11581975213728413 1102 48-49 -0.04295119843126827 1102 50-51 -0.01589056800187194 1102 52-53 0.08421978802628871 1102 54-55 -0.08702996663028273 1102 56-57 -0.03843095736385038 1102 58-59 -0.1299384261965173 1102 60-61 -0.18860676942205146 1102 62-63 -0.2517654053127458 1102 64-65 -0.17972931971565487 1102 66-67 -0.12517482425355553 1102 68-69 -0.05071932647201294 1102 70-71 0.023926024915205346 1102 72-73 -0.3828985741394888 1102 74-75 -0.313957876968459 1102 76-77 -0.2339460815994876 1102 78-79 -0.1290180185432206 1102 80-81 -0.04227982198219138 1102 82-83 -0.08630275207978855 1102 84-85 -0.4192444430954545 1102 86-87 -0.29598726776599094 1102 88-89 -0.30757615163271623 1102 90-91 -0.4024703458081582 1102 92-93 -0.36408403980336956 1102 94-95 -0.3591981810520899 1102 96-97 -0.2535679594143794 1102 98-99 -0.21281343830579758 1102 100-101 0.004232479424246094 1103 1 0.2608249914523384 1103 2 0.18577261439305204 1103 3 0.23808428293073547 1103 4 0.2566844074101269 1103 5 0.27420377962868514 1103 6 0.3119730317743574 1103 7 0.34549262308359374 1103 8 0.32494578441651356 1103 9 0.09541895860028404 1103 10-11 0.4450701410510547 1103 12-13 0.2630987529883768 1103 14-15 0.3037331748589054 1103 16-17 0.3965851093560602 1103 18-19 0.4969311340459157 1103 20-21 0.3933662148843382 1103 22-23 0.2502410178235621 1103 24-25 0.2820109866260978 1103 26-27 0.17856974916691826 1103 28-29 0.2640608489101979 1103 30-31 0.2724812768542222 1103 32-33 0.2810344980383306 1103 34-35 0.473712341843175 1103 36-37 0.43367517371543585 1103 38-39 0.39313074303333195 1103 40-41 0.2518287992503545 1103 42-43 0.21702943988191237 1103 44-45 0.3884848736747699 1103 46-47 0.4296395156179216 1103 48-49 0.46047555927919603 1103 50-51 0.5323277376431221 1103 52-53 0.5285908164365125 1103 54-55 0.5537688275261914 1103 56-57 0.52129100760542 1103 58-59 0.3930451460258553 1103 60-61 0.4062955259075025 1103 62-63 0.5352030309376019 1103 64-65 0.41752446546467326 1103 66-67 0.49475323594838017 1103 68-69 0.4168519602993186 1103 70-71 0.5047151661494524 1103 72-73 0.47317819568165476 1103 74-75 0.4503736848814768 1103 76-77 0.4978303173024763 1103 78-79 0.4338208132750907 1103 80-81 0.5092683262714957 1103 82-83 0.3488049438994665 1103 84-85 0.4175229202913542 1103 86-87 0.6032836575847433 1103 88-89 0.26966117727427985 1103 90-91 0.22680148784337462 1103 92-93 0.17255877357822058 1103 94-95 0.3632489890863013 1103 96-97 0.3708391220073679 1103 98-99 0.10954830406447513 1103 100-101 0.32070095248155184 1104 1 0.19836121913608196 1104 2 0.2617251609577025 1104 3 0.22191224490615724 1104 4 0.23665049829813967 1104 5 0.25104221381989333 1104 6 0.3028074277864121 1104 7 0.2993240629166607 1104 8 0.3083679963339705 1104 9 0.4660547302982536 1104 10-11 0.4125376810464161 1104 12-13 0.3994102434435902 1104 14-15 0.30135422362619835 1104 16-17 0.21445042931896552 1104 18-19 0.27556670286885776 1104 20-21 0.08488024224348578 1104 22-23 0.18850421436115994 1104 24-25 0.2412620879476819 1104 26-27 0.09458673270506068 1104 28-29 0.04356467455360047 1104 30-31 0.2751126142836995 1104 32-33 0.2076951750612963 1104 34-35 0.21245398532412452 1104 36-37 0.1953465258292013 1104 38-39 0.1515379808415105 1104 40-41 -0.05324694076046654 1104 42-43 0.3079512653707397 1104 44-45 0.1913905754291534 1104 46-47 0.2537202985223175 1104 48-49 0.30762250842519023 1104 50-51 0.23820241883950644 1104 52-53 0.28114124537228946 1104 54-55 0.2634136030111591 1104 56-57 0.08999077188396143 1104 58-59 0.21271822740773416 1104 60-61 0.36988670374195465 1104 62-63 0.14243214447034092 1104 64-65 0.26725444846182 1104 66-67 0.3085115441638564 1104 68-69 0.23605166661356947 1104 70-71 0.2471170132782845 1104 72-73 0.254263963515335 1104 74-75 0.3015938332700756 1104 76-77 0.27957059862251654 1104 78-79 0.39653165839449755 1104 80-81 0.37731744134994116 1104 82-83 0.35927112173392217 1104 84-85 0.373400016666654 1104 86-87 0.3211448717434884 1104 88-89 0.4471357695920801 1104 90-91 0.33547608595760536 1104 92-93 0.2268930536616871 1104 94-95 0.34130346586194094 1104 96-97 0.38485078249117777 1104 98-99 0.3170568537978369 1104 100-101 0.41373002157498817 1105 1 0.26912994283978975 1105 2 0.20359844651969183 1105 3 0.20861754336986849 1105 4 0.23272259559536224 1105 5 0.24054008290794116 1105 6 0.2279104486601966 1105 7 0.29508480961393246 1105 8 0.3793024438922643 1105 9 0.45246599259458975 1105 10-11 0.4065107125809462 1105 12-13 0.28540305300709434 1105 14-15 0.27774584022529325 1105 16-17 0.1885886961937402 1105 18-19 0.28224095284473805 1105 20-21 0.31865028060732214 1105 22-23 0.03067311294063302 1105 24-25 0.15469487208370225 1105 26-27 0.07841072407752847 1105 28-29 0.12701668389350118 1105 30-31 0.10866711888068181 1105 32-33 -0.14351077516713673 1105 34-35 -0.08542876875899452 1105 36-37 0.0011507874057556933 1105 38-39 0.05978601998202038 1105 40-41 7.58165760395002E-4 1105 42-43 -0.07515869117002438 1105 44-45 0.00445205774245494 1105 46-47 0.2935025262466837 1105 48-49 0.3846060312691151 1105 50-51 0.47757213020791767 1105 52-53 0.5553074008509427 1105 54-55 0.4621588631852376 1105 56-57 0.3403271665738714 1105 58-59 0.3520661720609084 1105 60-61 0.5281307234606203 1105 62-63 0.6430642758522467 1105 64-65 0.6688730696393392 1105 66-67 0.6494246826026355 1105 68-69 0.6998507437543111 1105 70-71 0.40352029238446363 1105 72-73 0.18278132865445684 1105 74-75 0.39577507085624575 1105 76-77 0.40417051155565176 1105 78-79 0.393826742085043 1105 80-81 0.5391124656767197 1105 82-83 0.38370688766258 1105 84-85 0.3842221553948342 1105 86-87 0.14878804354965425 1105 88-89 0.14789924331248017 1105 90-91 0.2692763627411807 1105 92-93 0.11548797509126629 1105 94-95 0.35060286208294045 1105 96-97 0.411205667758324 1105 98-99 0.5803461445739444 1105 100-101 0.8592294440149857 1106 1 0.1504187675592732 1106 2 0.11036087074822376 1106 3 0.15488521382940945 1106 4 0.17824798898310945 1106 5 0.07411277831587881 1106 6 0.1442658173096234 1106 7 0.22626598505996753 1106 8 0.19949823697648839 1106 9 0.2499309827081717 1106 10-11 0.23725148727375256 1106 12-13 0.22699472804507081 1106 14-15 0.14803515003494283 1106 16-17 0.2712028292510382 1106 18-19 0.18855043527518234 1106 20-21 -0.07649146502973281 1106 22-23 0.15522669630547625 1106 24-25 0.10400645147870335 1106 26-27 0.1392564361224018 1106 28-29 0.07420376572604681 1106 30-31 0.22292845133805628 1106 32-33 0.0940268002223732 1106 34-35 0.1641381832323603 1106 36-37 0.07877937767772636 1106 38-39 0.17714807803751143 1106 40-41 0.13199797218268117 1106 42-43 0.06728752557233264 1106 44-45 0.08042118767404816 1106 46-47 0.1860058779523257 1106 48-49 0.12183692400051882 1106 50-51 -0.13702421366371453 1106 52-53 0.020363402663981844 1106 54-55 0.10102610763121334 1106 56-57 0.048893495969188905 1106 58-59 0.15761281519879589 1106 60-61 0.18016845227102607 1106 62-63 0.21304346535698215 1106 64-65 0.2908648103745648 1106 66-67 0.44537010550322265 1106 68-69 0.40027032764626114 1106 70-71 0.24721128740496567 1106 72-73 0.006278058433771605 1106 74-75 0.12447709817662655 1106 76-77 0.17515607216582296 1106 78-79 0.06115738386823821 1106 80-81 0.29403530323772387 1106 82-83 0.1617580856824965 1106 84-85 0.27299120409610467 1106 86-87 0.26556808890113004 1106 88-89 0.04202736058971368 1106 90-91 0.1183253122854353 1106 92-93 0.014981049506136657 1106 94-95 0.18629432564197757 1106 96-97 0.16496543481319037 1106 98-99 0.1987016188991788 1106 100-101 -0.12470385417795526 1107 1 0.013327851864239904 1107 2 -0.04601609651464855 1107 3 -0.00876926398002098 1107 4 0.11130397262751757 1107 5 0.056192719369342115 1107 6 0.13455598371943722 1107 7 0.14640795489138014 1107 8 0.2222569972311561 1107 9 0.2525747557017368 1107 10-11 0.24956098773296986 1107 12-13 0.1906707400211829 1107 14-15 0.027250528024424625 1107 16-17 0.064384022399544 1107 18-19 0.09820163366453727 1107 20-21 0.1020332629392584 1107 22-23 0.13591988891433004 1107 24-25 0.09665629555058075 1107 26-27 0.17406460042550265 1107 28-29 0.14521073449372324 1107 30-31 0.059750060225695734 1107 32-33 -0.25314742415846325 1107 34-35 -0.12095537364118769 1107 36-37 -0.29123663025099944 1107 38-39 -0.36771498541822467 1107 40-41 -0.37798622744682575 1107 42-43 -0.24487940832556632 1107 44-45 -0.39799729642439274 1107 46-47 -0.43174969109450956 1107 48-49 -0.547259471231861 1107 50-51 -0.6819171136154552 1107 52-53 -0.6323073528998719 1107 54-55 -0.4698057338773225 1107 56-57 -0.7108552547896991 1107 58-59 -0.6445692704501127 1107 60-61 -0.5634556182572581 1107 62-63 -0.5519441849281463 1107 64-65 -0.5677205764880355 1107 66-67 -0.6568724314810623 1107 68-69 -0.6405946453452245 1107 70-71 -0.7429011572677879 1107 72-73 -0.6447899583992012 1107 74-75 -0.5855244844549006 1107 76-77 -0.6053828787310884 1107 78-79 -0.6850650562901279 1107 80-81 -0.8038991031716769 1107 82-83 -0.5530471728053392 1107 84-85 -0.6621157143387002 1107 86-87 -0.5837634438645303 1107 88-89 -0.7068596083855212 1107 90-91 -0.8084650071985742 1107 92-93 -0.5094349335156032 1107 94-95 -0.4673311977939427 1107 96-97 -0.4968787460322943 1107 98-99 -0.5249268103519995 1107 100-101 -0.7455533381362969 1108 1 0.008405681118382802 1108 2 0.029850116416533012 1108 3 0.059751054713395035 1108 4 0.07632605384196722 1108 5 -0.08398357386589339 1108 6 0.07306244081222957 1108 7 0.15342204820422012 1108 8 0.09866389485137717 1108 9 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-1.7014355976994402 2316 82-83 -1.5396265418833686 2316 84-85 -1.1047690335262637 2316 86-87 -0.8817648177500566 2316 88-89 -1.1985092032113656 2316 90-91 -1.194878355861423 2316 92-93 -1.6792665493070729 2316 94-95 -1.546909188547403 2316 96-97 -1.7958650338113742 2316 98-99 -3.2233114960784626 2316 100-101 -4.274407431050022 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2084.0 3 660.0 4 576.0 5 649.0 6 742.0 7 855.0 8 1032.0 9 1373.0 10 1760.0 11 2488.0 12 3771.0 13 5371.0 14 6210.0 15 8685.0 16 12409.0 17 19096.0 18 13873.0 19 7647.0 20 6767.0 21 6892.0 22 7358.0 23 8037.0 24 8868.0 25 10424.0 26 12228.0 27 14805.0 28 18423.0 29 22867.0 30 28976.0 31 38026.0 32 50932.0 33 71353.0 34 104491.0 35 158539.0 36 237432.0 37 329566.0 38 266360.0 39 40375.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.19947318085825 19.07320305385371 14.73285308143501 21.994470683853027 2 15.15740822341686 20.571924705175785 40.56833426358411 23.702332807823247 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21.229750384396993 22-23 21.680226001609167 26.08843243207956 30.570699759604008 21.66064180670726 24-25 21.702811800737873 26.358151454465222 30.712996059980206 21.226040684816695 26-27 21.438971016597613 26.301772685660385 30.868751417675288 21.39050488006671 28-29 20.90406986136799 26.754832146490337 31.008504865028225 21.332593127113448 30-31 21.092437605603507 26.490133445955315 30.885654633238218 21.531774315202956 32-33 20.85607224929152 26.687308886217682 30.687443353295603 21.7691755111952 34-35 21.752199403918784 26.57199123241032 30.259183058029798 21.416626305641103 36-37 21.772959395980493 26.100364653213916 30.53850725536854 21.588168695437055 38-39 21.424636342570683 26.45161079761489 30.544221461418207 21.57953139839622 40-41 21.46547492539106 26.538491163116163 30.714437711000347 21.28159620049243 42-43 21.24399783804672 26.813736497583466 30.093123265280546 21.849142399089263 44-45 21.34363937933956 26.88169761896525 29.854319342080615 21.920343659614577 46-47 21.779865828762777 27.05990055466801 29.23137929402626 21.928854322542954 48-49 21.834964428781518 27.253153889988035 28.869083947292324 22.042797733938123 50-51 21.675998913812595 27.51490896652924 28.93540579282542 21.873686326832743 52-53 21.937917601196897 27.628815037429078 28.658897989769994 21.77436937160403 54-55 21.37415667151125 27.387732175419504 28.947867440886633 22.290243712182605 56-57 21.82508366508899 27.803212922184777 28.384152490762084 21.98755092196415 58-59 21.943676084448338 27.24632288744519 28.542618277122017 22.267382750984453 60-61 21.6944978250312 27.236523287571085 29.211652140764606 21.857326746633106 62-63 21.78896155712743 27.337164707191675 29.12110668371192 21.752767051968977 64-65 21.47125134301391 27.610636278542717 28.86846753463768 22.049644843805687 66-67 21.43660583429353 27.70724733239544 28.45988882396911 22.39625800934192 68-69 21.52606214509053 27.589180160558733 28.786236964683155 22.098520729667584 70-71 21.54953347740785 27.620187888925273 28.521072611791887 22.309206021874992 72-73 21.56623765003641 27.432725359397036 28.70064788246004 22.30038910810651 74-75 21.431877862116963 28.106070201600108 28.357303188856953 22.104748747425973 76-77 21.62684726933351 27.658891032858225 28.429610106245633 22.284651591562636 78-79 21.621943725564336 27.69018433152639 28.517642003582537 22.170229939326735 80-81 21.651815092595765 28.09424666839758 28.19770964070458 22.05622859830207 82-83 21.640285007462133 28.05608729537086 28.20568911357821 22.097938583588792 84-85 21.578983789542818 27.917748462745195 28.085950480124872 22.417317267587116 86-87 21.704640154699025 27.93950834618085 28.217328105756266 22.138523393363858 88-89 21.7202091211064 27.8376478053774 27.955389412329996 22.486753661186203 90-91 21.502090226619416 27.89065853973063 28.20256834249355 22.40468289115641 92-93 21.72332668165878 27.944900239571275 28.240912270691055 22.090860808078897 94-95 21.454718148154797 27.66458153475066 28.25509872688906 22.625601590205484 96-97 21.51286068677373 27.49529964747356 28.241970231100666 22.74986943465204 98-99 21.43929519261914 27.75084372093995 27.816375761438554 22.99348532500235 100-101 21.532554112073445 27.572754404877408 28.03400392604202 22.86068755700713 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 463.0 1 374.0 2 435.0 3 597.5 4 481.5 5 441.0 6 825.0 7 1675.5 8 2777.5 9 3248.0 10 2708.5 11 2171.5 12 2180.0 13 2338.0 14 2517.0 15 2659.0 16 2825.5 17 2966.0 18 3098.5 19 3344.0 20 3653.5 21 4118.0 22 4841.0 23 5781.0 24 6790.5 25 8138.5 26 10368.0 27 12931.0 28 14823.0 29 17059.0 30 20474.5 31 24214.0 32 28143.0 33 31380.0 34 34794.0 35 37461.0 36 40528.5 37 44286.0 38 44798.5 39 44027.5 40 44055.0 41 45620.0 42 47152.0 43 48692.0 44 50458.0 45 52533.5 46 53117.5 47 55855.0 48 69487.0 49 68833.5 50 55535.5 51 49978.0 52 45736.0 53 44457.0 54 43730.0 55 41309.5 56 37770.5 57 33446.0 58 30338.5 59 27085.5 60 22868.0 61 19052.0 62 15894.0 63 12427.5 64 9229.5 65 7458.5 66 5710.5 67 4301.5 68 3069.5 69 2100.5 70 1700.5 71 1365.0 72 1125.5 73 1030.0 74 840.5 75 652.5 76 485.0 77 314.5 78 219.5 79 163.0 80 109.0 81 63.0 82 38.0 83 24.5 84 14.5 85 12.0 86 10.5 87 8.5 88 7.0 89 4.0 90 3.0 91 3.0 92 1.5 93 1.5 94 1.5 95 0.5 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011161836180798248 2 0.010509097222856829 3 0.015012996032652614 4 0.018668334197124554 5 0.027545584025127842 6 0.04105728045451519 7 0.005221911663531344 8 0.02173620729944922 9 0.03080927881483493 10-11 0.03746721618583739 12-13 0.028100412139378046 14-15 0.023009048267434985 16-17 0.025424182411818232 18-19 0.02180148119524336 20-21 0.046442376857531886 22-23 0.010345912483371475 24-25 0.002219312457000821 26-27 5.548281142502053E-4 28-29 3.26369478970709E-5 30-31 0.0024477710922803176 32-33 0.002121401613309608 34-35 0.0 36-37 1.631847394853545E-4 38-39 6.52738957941418E-5 40-41 0.0011749301242945523 42-43 0.003785885956060224 44-45 0.001631847394853545 46-47 5.548281142502053E-4 48-49 5.874650621472762E-4 50-51 0.0027415036233539554 52-53 0.0027415036233539554 54-55 0.00362270121657487 56-57 3.26369478970709E-4 58-59 0.004144892382928004 60-61 0.001958216873824254 62-63 3.590064268677799E-4 64-65 2.610955831765672E-4 66-67 0.013120053054622502 68-69 0.035345814572527785 70-71 0.04063300013185327 72-73 0.011063925337107035 74-75 0.023498602485891047 76-77 0.020855009706228306 78-79 0.0449737142021637 80-81 0.06680783234530413 82-83 0.03965389169494114 84-85 0.014164435387328771 86-87 0.016808028166991514 88-89 0.01710176069806515 90-91 0.030907189658526138 92-93 0.02033281853987517 94-95 0.00838769560954722 96-97 0.01344642253359321 98-99 0.04530008368113441 100-101 0.06184701626494936 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1532006.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.728190480347024 #Duplication Level Percentage of deduplicated Percentage of total 1 76.86729605984294 40.530734283526265 2 13.206578219837843 13.92717943938424 3 4.264787221466639 6.746235389149286 4 1.8631062280071335 3.9295288030192395 5 1.00231411603817 2.6425104815800635 6 0.5880283797465673 1.86034034490761 7 0.3804482557173351 1.4042243666765593 8 0.2645195643463828 1.1158110379707586 9 0.20430693512767 0.9695461492680918 >10 1.1714307392035253 11.829751558684878 >50 0.11847504764723156 4.386196550051046 >100 0.06238410472757095 6.106802916679607 >500 0.004464796440738875 1.598898494202939 >1k 0.0016122876036001495 1.834907682979152 >5k 2.480442467077153E-4 1.1173325019203033 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9749 0.6363552100970884 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7337 0.4789145734416184 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4568 0.2981711559876397 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3129 0.20424201993986968 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2937 0.19170943194739445 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2813 0.18361546886892088 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2215 0.1445816791840241 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2014 0.13146162612940157 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1956 0.12767574017334135 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1916 0.1250647843415757 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1782 0.11631808230516069 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 6.52738957941418E-5 0.0 3 0.0 0.0 0.0 6.52738957941418E-5 0.0 4 0.0 0.0 0.0 1.305477915882836E-4 0.0 5 0.0 0.0 0.0 1.305477915882836E-4 0.0 6 0.0 0.0 0.0 1.305477915882836E-4 0.0 7 0.0 0.0 0.0 1.305477915882836E-4 0.0 8 0.0 0.0 0.0 1.305477915882836E-4 0.0 9 0.0 0.0 0.0 1.305477915882836E-4 0.0 10-11 0.0 0.0 0.0 1.305477915882836E-4 0.0 12-13 0.0 0.0 0.0 1.305477915882836E-4 0.0 14-15 0.0 0.0 0.0 1.305477915882836E-4 0.0 16-17 0.0 0.0 0.0 2.2845863527949629E-4 0.0 18-19 0.0 0.0 0.0 2.610955831765672E-4 0.0 20-21 0.0 0.0 0.0 3.916433747648508E-4 0.0 22-23 0.0 0.0 0.0 4.5691727055899257E-4 0.0 24-25 0.0 0.0 0.0 5.874650621472762E-4 0.0 26-27 0.0 0.0 0.0 7.506498016326307E-4 0.0 28-29 0.0 0.0 0.0 0.001599210446956474 0.0 30-31 0.0 0.0 0.0 0.004079618487133863 0.0 32-33 0.0 0.0 0.0 0.007506498016326307 0.0 34-35 0.0 0.0 0.0 0.012075670721916232 0.0 36-37 0.0 0.0 0.0 0.019647442634036682 0.0 38-39 6.52738957941418E-5 0.0 0.0 0.032767495688659186 0.0 40-41 6.52738957941418E-5 0.0 0.0 0.045561179264310976 0.0 42-43 6.52738957941418E-5 0.0 0.0 0.06086790782803722 0.0 44-45 6.52738957941418E-5 0.0 0.0 0.07914459865039694 0.0 46-47 6.52738957941418E-5 0.0 0.0 0.10212100996993484 0.0 48-49 6.52738957941418E-5 0.0 0.0 0.12956868315137146 0.0 50-51 6.52738957941418E-5 0.0 0.0 0.1596925860603679 0.0 52-53 6.52738957941418E-5 0.0 0.0 0.1892942978030112 0.0 54-55 6.52738957941418E-5 0.0 0.0 0.22490120795871557 0.0 56-57 6.52738957941418E-5 0.0 0.0 0.2728448844195127 0.0 58-59 6.52738957941418E-5 0.0 0.0 0.3262715681270178 0.0 60-61 6.52738957941418E-5 0.0 0.0 0.3884449538709378 0.0 62-63 6.52738957941418E-5 0.0 0.0 0.45603607296577164 0.0 64-65 6.52738957941418E-5 0.0 0.0 0.5245736635496205 0.0 66-67 6.52738957941418E-5 0.0 0.0 0.606720861406548 0.0 68-69 6.52738957941418E-5 0.0 0.0 0.7041095139314075 0.0 70-71 6.52738957941418E-5 0.0 0.0 0.8132801046471096 3.26369478970709E-5 72-73 6.52738957941418E-5 0.0 0.0 0.9255512054130337 6.52738957941418E-5 74-75 6.52738957941418E-5 0.0 0.0 1.0446434282894455 6.52738957941418E-5 76-77 6.52738957941418E-5 0.0 0.0 1.1772147106473474 6.52738957941418E-5 78-79 6.52738957941418E-5 0.0 0.0 1.3334151432827288 6.52738957941418E-5 80-81 6.52738957941418E-5 0.0 0.0 1.505281310908704 6.52738957941418E-5 82-83 6.52738957941418E-5 0.0 0.0 1.6820430207192398 6.52738957941418E-5 84-85 6.52738957941418E-5 0.0 0.0 1.8743072807808847 6.52738957941418E-5 86-87 6.52738957941418E-5 0.0 0.0 2.0819109063541523 6.52738957941418E-5 88-89 6.52738957941418E-5 0.0 0.0 2.3129152235696204 6.52738957941418E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9560 0.0 25.09466 1 ATGGGCG 210 1.2623786E-9 24.888931 5 GAGTACT 7580 0.0 24.097664 12-13 TACATGG 9790 0.0 24.068375 2 GTATCAA 15210 0.0 23.831049 1 ACATGGG 9565 0.0 23.296593 3 GTACTTT 8220 0.0 22.369215 14-15 AGTACTT 7875 0.0 22.018621 12-13 GGTATCA 11445 0.0 21.501154 1 CCGTCTA 135 3.7893633E-4 21.117191 9 ATCAACG 17325 0.0 20.842262 3 TCAACGC 17450 0.0 20.611954 4 TATCAAC 17585 0.0 20.504406 2 CATTCCG 255 1.1887096E-8 20.4961 9 CAACGCA 17570 0.0 20.444801 5 AACGCAG 17875 0.0 20.202284 6 CATGGGG 3745 0.0 20.172697 4 GGATCGT 145 6.1340537E-4 19.661478 6 ACTTTTT 9595 0.0 19.388039 16-17 CATGGGA 5290 0.0 19.310854 4 >>END_MODULE