FastQCFastQC Report
Thu 26 May 2016
SRR936423_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936423_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1532006
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93050.6073736003644894No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83860.5473868901296731No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51580.3366827545061834No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT51000.3328968685501232No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA27020.17637006643577113No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23300.1520881772003504No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA23270.15189235551296795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGGT5600.022.0519814
GAGTACT96850.021.82483712-13
TAAGGTG6600.021.5893525
GTACTTT101700.020.66793614-15
AGTACTT99350.020.00866912-13
GGTATCA161700.019.5964471
GTACATG94550.018.7416931
GTATCAA199100.018.5400661
ACTTTTT116500.018.28635616-17
CGCGCTT2101.8189894E-1218.09481862-63
TACATGG96200.018.071012
TACTTTT112250.017.58285914-15
GTAAGCG1507.6957804E-817.4159894-95
ACATGGG96150.017.3388423
TCGCGCT2205.456968E-1217.27204360-61
ATTCGCG2156.730261E-1116.56911380-81
CTCGCGC2301.0913936E-1116.52108660-61
GTGTAGC9000.016.3636681
CGCGGAA2056.148184E-1016.21834282-83
CATGGGA53900.015.9496844
Produced by FastQC (version 0.11.5)