##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936423_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1532006 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.214133626108513 31.0 31.0 33.0 30.0 34.0 2 31.347536497898833 31.0 31.0 34.0 30.0 34.0 3 31.41488218714548 31.0 31.0 34.0 28.0 34.0 4 35.27319214154514 37.0 35.0 37.0 33.0 37.0 5 35.10543888209315 37.0 35.0 37.0 32.0 37.0 6 35.19342939910157 37.0 35.0 37.0 32.0 37.0 7 35.101582500329634 37.0 35.0 37.0 32.0 37.0 8 35.06050433222846 37.0 35.0 37.0 32.0 37.0 9 36.506789790640504 38.0 35.0 39.0 32.0 39.0 10-11 36.3606418643269 38.0 35.0 39.0 32.0 39.0 12-13 36.190308327774176 38.0 35.0 39.0 32.0 39.0 14-15 36.98550266774412 38.0 36.0 40.0 31.0 41.0 16-17 36.94251132175722 38.0 36.0 40.0 31.5 41.0 18-19 37.1611726716475 39.0 36.0 40.0 31.0 41.0 20-21 37.183491122097436 39.0 36.0 40.0 31.0 41.0 22-23 37.04871162384482 39.0 36.0 40.0 31.0 41.0 24-25 36.874639198541 39.0 36.0 40.0 31.0 41.0 26-27 36.54552168855736 38.0 35.0 40.0 30.0 41.0 28-29 36.46933660834226 38.0 35.0 40.0 30.0 41.0 30-31 36.254688297565416 38.0 35.0 40.0 30.0 41.0 32-33 35.939400041514205 38.0 34.5 40.0 29.5 41.0 34-35 35.66927740491878 38.0 34.0 40.0 29.0 41.0 36-37 35.38246553864671 38.0 34.0 40.0 27.0 41.0 38-39 35.080105430396486 38.0 34.0 40.0 27.0 41.0 40-41 34.74077614578533 38.0 33.0 40.0 25.5 41.0 42-43 34.33890010874631 37.5 33.0 40.0 24.5 41.0 44-45 33.90862666334205 37.0 33.0 40.0 24.0 41.0 46-47 33.32322654088822 37.0 32.0 40.0 21.5 41.0 48-49 32.743058447551775 37.0 31.0 39.0 18.5 40.0 50-51 32.30880949552417 36.0 31.0 39.0 15.0 40.0 52-53 31.928064250401107 36.0 30.0 39.0 12.5 40.0 54-55 31.484255283595495 35.5 30.0 39.0 9.0 40.0 56-57 31.029991396900535 35.0 29.0 38.5 8.0 40.0 58-59 30.541459041283126 35.0 28.0 38.0 7.0 40.0 60-61 30.02996430823378 34.0 28.0 38.0 4.0 40.0 62-63 29.55946810913273 34.0 27.0 38.0 2.0 40.0 64-65 29.068059459297157 34.0 26.0 37.5 2.0 39.0 66-67 28.465135906778436 33.0 26.0 37.0 2.0 39.0 68-69 27.88555495213465 32.5 25.5 36.0 2.0 39.0 70-71 27.246051582043414 32.0 23.5 36.0 2.0 38.5 72-73 26.51858674182738 31.0 22.0 35.0 2.0 38.0 74-75 25.843660207597097 31.0 20.5 35.0 2.0 37.0 76-77 24.880688783203198 29.5 20.5 33.5 2.0 36.0 78-79 25.54167183418342 31.0 21.0 34.0 2.0 36.0 80-81 25.260640950492363 31.0 20.0 34.0 2.0 36.0 82-83 24.662571491234367 31.0 18.0 34.0 2.0 35.5 84-85 24.000762725472356 30.0 14.0 34.0 2.0 35.0 86-87 23.327433769841633 30.0 7.5 34.0 2.0 35.0 88-89 22.52199240734044 29.0 2.0 34.0 2.0 35.0 90-91 21.60705277916666 29.0 2.0 33.5 2.0 35.0 92-93 20.668916766644514 27.5 2.0 33.0 2.0 34.0 94-95 19.542177054136864 26.0 2.0 32.5 2.0 34.0 96-97 18.287430010065236 25.0 2.0 32.0 2.0 34.0 98-99 16.76868236808472 18.0 2.0 32.0 2.0 34.0 100-101 13.443332793735795 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3576284942187691 1101 2 0.4950355095917516 1101 3 0.3407523112924373 1101 4 0.3701133379380366 1101 5 0.2635268713064818 1101 6 0.3165296204803383 1101 7 0.3824331319757519 1101 8 0.3214326311321045 1101 9 0.3948128970088831 1101 10-11 0.4509370666752375 1101 12-13 0.364957582318695 1101 14-15 0.4478278500507855 1101 16-17 0.557224753774193 1101 18-19 0.3918264680930008 1101 20-21 0.28887098957329016 1101 22-23 0.47232672435877987 1101 24-25 0.5408392159053861 1101 26-27 0.25205404727574887 1101 28-29 0.35104157107258516 1101 30-31 0.6485583319000483 1101 32-33 0.7264195932882132 1101 34-35 0.5253802291498815 1101 36-37 0.527486939881264 1101 38-39 0.5212783961007972 1101 40-41 0.4171410394343198 1101 42-43 0.699384436170547 1101 44-45 0.6986765613962334 1101 46-47 0.7364023549122436 1101 48-49 0.6702421108181724 1101 50-51 0.7354529585906207 1101 52-53 0.7273419085190085 1101 54-55 0.7794410788085919 1101 56-57 0.6506705329686895 1101 58-59 0.8604880316351284 1101 60-61 0.47833282405460764 1101 62-63 0.5124353791045131 1101 64-65 0.8514661410752637 1101 66-67 0.5539781989654742 1101 68-69 0.5122992788715592 1101 70-71 0.6016081418534576 1101 72-73 0.5617547342007114 1101 74-75 0.8897254694389183 1101 76-77 0.9897170323174116 1101 78-79 0.9789717573297843 1101 80-81 1.053310221420471 1101 82-83 0.658909577730018 1101 84-85 0.6805856013138225 1101 86-87 0.6740329634151685 1101 88-89 0.7190223119296562 1101 90-91 1.251599647622637 1101 92-93 1.1969831100954202 1101 94-95 1.240868060657771 1101 96-97 0.9983825184458972 1101 98-99 0.4212125953894734 1101 100-101 0.23505494658534332 1102 1 0.36265532065201 1102 2 0.4961569352132784 1102 3 0.39098581643827046 1102 4 0.420789580858802 1102 5 0.21374729757193478 1102 6 0.17241390891560826 1102 7 0.28393443426696763 1102 8 0.23966959805726873 1102 9 0.4540973408589508 1102 10-11 0.43918242293627685 1102 12-13 0.41834165424547365 1102 14-15 0.5811275631991251 1102 16-17 0.5819239542539947 1102 18-19 0.5004710188835375 1102 20-21 0.3385456966093159 1102 22-23 0.3447096963634948 1102 24-25 0.4233773199272619 1102 26-27 0.47932507407979585 1102 28-29 0.5244547111055695 1102 30-31 0.6177602197362759 1102 32-33 0.7030341722336928 1102 34-35 0.7558047684286464 1102 36-37 0.7831164552616201 1102 38-39 0.7209796438377722 1102 40-41 0.9347361102281795 1102 42-43 1.0383306908092749 1102 44-45 1.0487142368196558 1102 46-47 0.778318251820032 1102 48-49 0.8501128007526759 1102 50-51 0.8008195236701354 1102 52-53 0.8067541306738093 1102 54-55 0.9720541036060375 1102 56-57 1.2465589041355294 1102 58-59 1.2500414526755108 1102 60-61 1.1102021083040405 1102 62-63 1.0591670434863012 1102 64-65 0.8740361940918611 1102 66-67 0.8495536785391522 1102 68-69 0.8884007474177373 1102 70-71 0.6433197548976928 1102 72-73 0.4269518335171334 1102 74-75 0.598586057411179 1102 76-77 0.6964075276829504 1102 78-79 0.7634852730565527 1102 80-81 0.9129736876890355 1102 82-83 1.0384835773871401 1102 84-85 0.8240087802711997 1102 86-87 0.9840541252792754 1102 88-89 0.9526569210036868 1102 90-91 1.064102703779934 1102 92-93 1.2330124798406707 1102 94-95 1.2458291981197505 1102 96-97 1.209098598929323 1102 98-99 1.0700884008157985 1102 100-101 1.193855736973898 1103 1 0.24487364054486704 1103 2 0.48504538875101844 1103 3 0.35921369189301444 1103 4 0.23053083979932865 1103 5 0.2654883855381982 1103 6 0.26253011205203336 1103 7 0.3051901571556712 1103 8 0.3207155568904625 1103 9 0.09857890888296339 1103 10-11 0.4369233957323715 1103 12-13 0.35911539592234476 1103 14-15 0.5449365255752809 1103 16-17 0.5873982475603512 1103 18-19 0.3338445727261856 1103 20-21 0.3737583814752412 1103 22-23 0.45672737676509456 1103 24-25 0.5010201190609891 1103 26-27 0.21146716794466158 1103 28-29 0.5165741627530949 1103 30-31 0.6666429800683531 1103 32-33 0.5976245285725597 1103 34-35 0.6522492754053459 1103 36-37 0.5373207600137562 1103 38-39 0.3840723249517666 1103 40-41 0.6195975854511602 1103 42-43 0.6339621498371883 1103 44-45 0.8135173136440912 1103 46-47 0.8234904846489925 1103 48-49 0.8581931159152489 1103 50-51 1.081868124293237 1103 52-53 0.9373235580999761 1103 54-55 1.0036377408936197 1103 56-57 1.1422347689803694 1103 58-59 0.9771990232736378 1103 60-61 0.5922840940276899 1103 62-63 0.8693501343686272 1103 64-65 0.9363762025455884 1103 66-67 0.8377263257432475 1103 68-69 1.0488348677076331 1103 70-71 0.6537171275931044 1103 72-73 1.0064875196463738 1103 74-75 0.9882137075123403 1103 76-77 0.7427261192349235 1103 78-79 0.3843719467996536 1103 80-81 0.6326122492730164 1103 82-83 0.7619782617496433 1103 84-85 0.7315336665258165 1103 86-87 0.7577910528716707 1103 88-89 0.7014891904971279 1103 90-91 0.8633379501253593 1103 92-93 0.9722238847742304 1103 94-95 1.212951083775959 1103 96-97 1.4685216260762388 1103 98-99 1.269797477412272 1103 100-101 1.076296976442359 1104 1 0.3067070511639258 1104 2 0.4184589060243802 1104 3 0.35462968230958225 1104 4 0.3432424094724098 1104 5 0.18599035632419003 1104 6 0.20872234097235065 1104 7 0.2439967689494793 1104 8 0.22031656933346255 1104 9 0.30854490316382055 1104 10-11 0.37957376477171323 1104 12-13 0.44632026105875866 1104 14-15 0.5681777922883242 1104 16-17 0.5139900919841836 1104 18-19 0.5099944394083096 1104 20-21 0.4277303847212437 1104 22-23 0.3951173701958055 1104 24-25 0.40644412787155915 1104 26-27 0.5350673302446864 1104 28-29 0.6995505326425757 1104 30-31 0.6182995512871443 1104 32-33 0.8333544439446925 1104 34-35 0.9106854953605179 1104 36-37 0.8994430260312214 1104 38-39 0.9308990213734347 1104 40-41 0.7873100245886491 1104 42-43 1.059073489585245 1104 44-45 1.2845532804885167 1104 46-47 1.0750458598711887 1104 48-49 1.0889054970884402 1104 50-51 1.0453155989821639 1104 52-53 0.9422625989841293 1104 54-55 0.8427892402366943 1104 56-57 1.2437121339235695 1104 58-59 1.0155532720563833 1104 60-61 1.169909151991984 1104 62-63 1.095989316299221 1104 64-65 1.1091073834714749 1104 66-67 1.3831256514252956 1104 68-69 1.3232692328053979 1104 70-71 0.8746190520076631 1104 72-73 0.9137543099701979 1104 74-75 1.049029120218112 1104 76-77 1.0073282909638053 1104 78-79 1.1640291363042543 1104 80-81 1.3480015278107693 1104 82-83 1.2865031960567777 1104 84-85 1.2449910434483016 1104 86-87 1.562407908888364 1104 88-89 1.6743570705496857 1104 90-91 1.6744612981429796 1104 92-93 1.5589191471592656 1104 94-95 1.568107973798373 1104 96-97 1.7118714309297793 1104 98-99 1.4461502191233748 1104 100-101 1.1474747142891797 1105 1 0.2323418724666162 1105 2 0.3405615830830051 1105 3 0.3095255854674903 1105 4 0.3017282747117065 1105 5 0.2577089003288222 1105 6 0.2606532712273406 1105 7 0.28971645570665316 1105 8 0.2338994324975019 1105 9 0.37544756142391833 1105 10-11 0.3577320718654349 1105 12-13 0.19587518738584464 1105 14-15 0.43792950950901144 1105 16-17 0.49353973740933554 1105 18-19 0.5883147292024375 1105 20-21 0.3928884902255021 1105 22-23 0.18549617287806086 1105 24-25 0.45738241552364656 1105 26-27 0.5221977598652288 1105 28-29 0.42289496370268864 1105 30-31 0.588348365619531 1105 32-33 0.5654921332594611 1105 34-35 0.1858166212686001 1105 36-37 0.38546811464401287 1105 38-39 0.5310826471145518 1105 40-41 0.5138163493035179 1105 42-43 0.6200018956433127 1105 44-45 0.5899600830817278 1105 46-47 0.6638860587892879 1105 48-49 0.5737229040295446 1105 50-51 1.1118503255501722 1105 52-53 1.0597744160843519 1105 54-55 0.9632574966980734 1105 56-57 1.2379790470323222 1105 58-59 1.2295506922059332 1105 60-61 1.176126424622563 1105 62-63 0.9666831241634348 1105 64-65 1.0276684861040053 1105 66-67 1.1503416081590032 1105 68-69 1.227809509885791 1105 70-71 1.0372649570206853 1105 72-73 1.1460965260196865 1105 74-75 1.1621296218419594 1105 76-77 0.9423860284126953 1105 78-79 0.9552149152187361 1105 80-81 1.1020337808884122 1105 82-83 1.1730607343896935 1105 84-85 1.4129176958017027 1105 86-87 1.5681799532528906 1105 88-89 1.4527038885280028 1105 90-91 1.7894679332808927 1105 92-93 1.5565416591371424 1105 94-95 1.6611186320683622 1105 96-97 1.4482871764274101 1105 98-99 1.3220176412764495 1105 100-101 1.0538466109863265 1106 1 0.12677333024531023 1106 2 0.2886690311904516 1106 3 0.2637294455629018 1106 4 0.21104760791008204 1106 5 0.21601947516703035 1106 6 0.13760843285403723 1106 7 0.15980810082437102 1106 8 0.2617959364106923 1106 9 0.23250002118753343 1106 10-11 0.24242114307693186 1106 12-13 0.31403618002546096 1106 14-15 0.29312022888116473 1106 16-17 0.31539196697794125 1106 18-19 0.27342007397931667 1106 20-21 0.1858891548372199 1106 22-23 0.18865578409293704 1106 24-25 0.25420889457119245 1106 26-27 0.22021672039722517 1106 28-29 0.29613094160498576 1106 30-31 0.21897432927469396 1106 32-33 0.2744337390988747 1106 34-35 0.2648634000025538 1106 36-37 0.4659518615771816 1106 38-39 0.4922240286156878 1106 40-41 0.3497525860478916 1106 42-43 0.5361823580959282 1106 44-45 0.620637420458749 1106 46-47 0.3852445722489435 1106 48-49 0.6231039047921954 1106 50-51 0.4641836601566993 1106 52-53 0.5658778306862047 1106 54-55 0.744705439903047 1106 56-57 0.49859628288417923 1106 58-59 0.6136133477753667 1106 60-61 0.5076771152712745 1106 62-63 0.5756005073248254 1106 64-65 0.5491022140028008 1106 66-67 0.1403560399096122 1106 68-69 0.17145167758948077 1106 70-71 0.2303521675740079 1106 72-73 0.3682923292895275 1106 74-75 0.27580209813174505 1106 76-77 0.12176896444318075 1106 78-79 0.029599226307947646 1106 80-81 0.14202379898062034 1106 82-83 0.18376375226164043 1106 84-85 0.41409572643750536 1106 86-87 0.4287840114586139 1106 88-89 0.5271470852339739 1106 90-91 0.6520284263158338 1106 92-93 0.5612485297199292 1106 94-95 0.39335745685150414 1106 96-97 0.14903429166879079 1106 98-99 0.4418067416442817 1106 100-101 0.3455377868015894 1107 1 0.05108073436637639 1107 2 0.03982227662287485 1107 3 -0.07904908461346949 1107 4 -0.11500691763774284 1107 5 -0.009683239175764413 1107 6 -0.059433083981531354 1107 7 -0.03560408388170089 1107 8 -0.07035042327734686 1107 9 -0.12405648394693713 1107 10-11 -3.7399175808872087E-4 1107 12-13 0.08349087265766286 1107 14-15 0.06736692221907248 1107 16-17 0.07828068463129512 1107 18-19 -0.0161625783035646 1107 20-21 -0.1009984835133153 1107 22-23 -0.06280685283064003 1107 24-25 -0.03609372533259858 1107 26-27 0.02654153865404396 1107 28-29 0.15284895298955803 1107 30-31 0.04463449417971077 1107 32-33 -0.005314544653984399 1107 34-35 -0.06383100682196385 1107 36-37 0.11474257542183608 1107 38-39 0.1268378665230827 1107 40-41 0.09678580055413732 1107 42-43 0.03385965174413741 1107 44-45 0.044960391447297354 1107 46-47 0.12531161355822462 1107 48-49 -0.07158377195660393 1107 50-51 0.19579594656631372 1107 52-53 -0.04482270429514301 1107 54-55 0.04027484997066111 1107 56-57 0.2104198376302584 1107 58-59 0.00719759306768708 1107 60-61 -0.08123564281441631 1107 62-63 -0.3712224247465521 1107 64-65 -0.37042681042161263 1107 66-67 -0.22793204572891312 1107 68-69 -0.20915044884461764 1107 70-71 -0.2578961447181065 1107 72-73 0.050490189364143845 1107 74-75 -0.16242273413525865 1107 76-77 -0.21083986093288942 1107 78-79 0.07109725191025618 1107 80-81 0.17145425918911172 1107 82-83 0.3458479223140998 1107 84-85 -0.09288471638798868 1107 86-87 0.0735805265558831 1107 88-89 0.1116245833270284 1107 90-91 -0.02296067687693082 1107 92-93 0.29997941490389834 1107 94-95 0.24594116768137653 1107 96-97 0.4924179838184166 1107 98-99 0.5309173286708493 1107 100-101 0.22987725866966713 1108 1 -0.03809007866928482 1108 2 0.07988038291708222 1108 3 -0.07400283129031848 1108 4 0.08336581819798994 1108 5 0.024617709747460026 1108 6 -0.07081406136833834 1108 7 -0.01856021075030867 1108 8 0.007256373431516749 1108 9 0.05740365949526449 1108 10-11 -0.03796981298594204 1108 12-13 -0.09333177015041372 1108 14-15 -0.040559897852972426 1108 16-17 -0.021643666128909445 1108 18-19 0.04753274481158343 1108 20-21 -0.1761188299025136 1108 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2316 70-71 -1.5772224303220526 2316 72-73 -1.5706805763939222 2316 74-75 -1.921758885524568 2316 76-77 -2.10427772646079 2316 78-79 -1.9932392657005558 2316 80-81 -1.9218839496991968 2316 82-83 -1.8093040374148543 2316 84-85 -1.8857908948776902 2316 86-87 -1.9413385606869547 2316 88-89 -1.9685690479700355 2316 90-91 -2.1150204397873473 2316 92-93 -2.2271811809939734 2316 94-95 -2.163240060914088 2316 96-97 -1.841840428733434 2316 98-99 -1.5756842023569888 2316 100-101 -1.3436586321474948 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 30.0 4 44.0 5 113.0 6 282.0 7 678.0 8 1477.0 9 2622.0 10 4264.0 11 6225.0 12 8677.0 13 11953.0 14 15579.0 15 20292.0 16 25113.0 17 30229.0 18 26729.0 19 21377.0 20 21189.0 21 22530.0 22 24321.0 23 27217.0 24 30818.0 25 35241.0 26 41857.0 27 49005.0 28 57631.0 29 67386.0 30 77719.0 31 89260.0 32 103523.0 33 120635.0 34 139781.0 35 157325.0 36 157830.0 37 108145.0 38 24531.0 39 370.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.199913570084284 22.07892670233968 16.351508639401207 20.369651088174834 2 17.023416916685598 23.331596909426562 37.42692005399887 22.218066119888974 3 20.87180561729616 26.393406560409726 30.935991910947315 21.7987959113468 4 14.219106763568194 19.16453111680011 36.632523978428225 29.983838141203474 5 12.935232450357065 39.141284906719584 33.717834902150216 14.205647740773136 6 30.03232363319726 38.38803503380535 16.68929495054197 14.890346382455421 7 26.931047875170123 34.792771564061475 20.561290596310062 17.71488996445834 8 26.61846741524334 34.633159570593236 19.210900446091124 19.537472568072303 9 26.410275155580333 16.925912822795734 20.58151208285085 36.08229993877309 10-11 24.917004241497747 27.61891924705256 26.572839793055643 20.891236718394055 12-13 26.649993537884313 25.10466669190591 27.523031894130963 20.722307876078812 14-15 23.118497676730524 26.16487396707769 27.134455896065308 23.582172460126483 16-17 21.063278480603646 29.679744874263235 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42-43 21.411383194303603 26.87203836326234 30.543415362994388 21.173163079439668 44-45 21.804590543183377 26.73183274453435 30.167788126365924 21.295788585916345 46-47 22.10725676009102 27.259553813757908 29.43376200876498 21.199427417386094 48-49 22.276937216502315 27.411602900561956 28.949580901565884 21.36187898136985 50-51 22.01423244100985 27.668370547385006 28.917756151627326 21.39964085997782 52-53 22.397102610020433 27.864793689554073 28.538945345608795 21.199158354816696 54-55 21.818147401003067 27.545806817147078 28.803701294611077 21.832344487238778 56-57 22.259254520363015 27.921148731661017 28.322004953500546 21.497591794475422 58-59 22.5082834195836 27.425756017065538 28.384746236233465 21.681214327117395 60-61 22.024911828810488 27.546716206592457 28.985262698903302 21.443109265693757 62-63 22.163773017638004 27.493163368645458 28.967995851187123 21.37506776252942 64-65 21.903527570218824 27.72668494824607 28.739569741005155 21.630217740529947 66-67 21.879139826637644 27.873094761825033 28.420388206607928 21.827377204929395 68-69 21.97538552119816 27.688661340894765 28.560341178386594 21.77561195952048 70-71 22.011915586937633 27.824174920346252 28.43023532859904 21.733674164117073 72-73 22.10995199966579 27.548281834776628 28.433645992068374 21.908120173489205 74-75 21.922834640529278 28.359754684526784 27.960709051801512 21.756701623142426 76-77 22.0019497497212 27.939123683127487 28.17966026573907 21.879266301412247 78-79 22.012850607606705 27.9277791413606 28.169470570111077 21.88989968092162 80-81 21.93924942345439 28.271459801079523 27.975663998422945 21.813626777043137 82-83 22.106314779765523 28.089527892037015 28.005289922547416 21.798867405650046 84-85 21.827152386830242 28.11496498484477 27.906705017919624 22.151177610405362 86-87 22.09714236105898 28.06914364826609 27.758699581396595 22.075014409278335 88-89 22.027397904082857 27.992755773008565 27.663965257998075 22.315881064910506 90-91 21.924216654214863 28.01296945995072 27.844356036089668 22.21845784974475 92-93 22.013192778339327 28.081015708812384 27.875920911216213 22.029870601632076 94-95 21.744959696501503 27.910980475861308 27.7856541869098 22.55840564072739 96-97 21.80116905623676 27.7851899961162 27.742305018586755 22.671335929060284 98-99 21.789918064487704 27.995541768181738 27.317733873586608 22.89680629374395 100-101 21.855680600948972 28.07463819626535 27.346494105329256 22.723187097456425 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 770.0 1 546.0 2 436.5 3 548.0 4 447.0 5 397.5 6 669.5 7 1365.5 8 2216.5 9 2670.0 10 2449.5 11 2078.0 12 2095.5 13 2272.5 14 2458.0 15 2746.5 16 3089.0 17 3391.5 18 3624.0 19 3931.5 20 4419.5 21 4969.0 22 5673.0 23 6595.5 24 7673.0 25 9146.5 26 11426.0 27 13958.0 28 16010.5 29 18463.5 30 21687.0 31 25126.0 32 29203.0 33 32489.0 34 35197.0 35 37792.5 36 40979.5 37 44726.5 38 45488.0 39 44376.5 40 44628.5 41 46257.0 42 47359.0 43 48655.0 44 50362.0 45 51954.5 46 52792.5 47 56085.0 48 66314.5 49 66242.0 50 56079.5 51 49774.5 52 45373.0 53 43685.5 54 42414.0 55 39585.5 56 35936.5 57 32142.0 58 29055.5 59 26089.5 60 22234.5 61 18564.0 62 15371.0 63 11990.5 64 9250.0 65 7337.5 66 5584.0 67 4257.0 68 3080.0 69 2166.5 70 1633.5 71 1302.5 72 1106.5 73 973.0 74 818.5 75 677.0 76 526.0 77 364.5 78 261.0 79 187.0 80 117.5 81 78.5 82 54.0 83 29.0 84 16.5 85 12.5 86 6.0 87 2.0 88 1.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008420332557444291 2 0.0079634152868853 3 0.0038511598518543664 4 2.610955831765672E-4 5 0.0043080771224133585 6 0.0 7 6.52738957941418E-5 8 0.0020887646654125375 9 0.0 10-11 0.0 12-13 0.0 14-15 3.590064268677799E-4 16-17 0.00362270121657487 18-19 0.0017297582385447576 20-21 3.26369478970709E-4 22-23 2.610955831765672E-4 24-25 4.569172705589926E-4 26-27 9.79108436912127E-5 28-29 6.52738957941418E-5 30-31 5.221911663531344E-4 32-33 0.00326369478970709 34-35 9.79108436912127E-4 36-37 0.0012402040200886941 38-39 0.004895542184560635 40-41 3.26369478970709E-5 42-43 0.001272840967985765 44-45 0.004830268288766493 46-47 0.0 48-49 0.0017623951864418287 50-51 8.485606453238433E-4 52-53 4.242803226619217E-4 54-55 9.79108436912127E-5 56-57 0.010443823327062687 58-59 0.001958216873824254 60-61 0.0028067775191480972 62-63 9.79108436912127E-5 64-65 0.002937325310736381 66-67 8.159236974267724E-4 68-69 0.0014033887595740486 70-71 0.004112255435030934 72-73 0.0024477710922803176 74-75 0.006592663475208322 76-77 9.46471489015056E-4 78-79 0.006135746204649329 80-81 0.0024151341443832464 82-83 0.0023824971964861758 84-85 0.0017297582385447576 86-87 4.569172705589926E-4 88-89 0.0017950321343388994 90-91 0.006494752631517109 92-93 0.002056127717515467 94-95 3.26369478970709E-4 96-97 6.52738957941418E-5 98-99 5.548281142502053E-4 100-101 0.001892942978030112 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1532006.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.251349242617074 #Duplication Level Percentage of deduplicated Percentage of total 1 78.23534403556151 42.443729723895466 2 12.561870374128986 13.62996833614713 3 3.988052266940779 6.490716489948449 4 1.6970042020805554 3.6825907053304374 5 0.9039266944777047 2.4519621395917195 6 0.535142887828868 1.7419334221383944 7 0.3497227343866066 1.3281051140903561 8 0.24604227169474835 1.0678500168126936 9 0.18583537704330116 0.9073637947448601 >10 1.1180011179901501 11.546985451514784 >50 0.1138927635936359 4.247097190892118 >100 0.058769538301139 5.762970219053008 >500 0.004102924963218771 1.4612327296879417 >1k 0.0018101139543612228 1.4077458506487368 >5k 4.8269705449632605E-4 1.8297488155039552 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9305 0.6073736003644894 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8386 0.5473868901296731 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5158 0.3366827545061834 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5100 0.3328968685501232 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2702 0.17637006643577113 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2330 0.1520881772003504 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2327 0.15189235551296795 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.26369478970709E-5 0.0 12-13 0.0 0.0 0.0 6.52738957941418E-5 0.0 14-15 0.0 0.0 0.0 6.52738957941418E-5 0.0 16-17 0.0 0.0 0.0 9.79108436912127E-5 0.0 18-19 0.0 0.0 0.0 1.305477915882836E-4 0.0 20-21 0.0 0.0 0.0 1.958216873824254E-4 0.0 22-23 0.0 0.0 0.0 2.610955831765672E-4 0.0 24-25 0.0 0.0 0.0 3.916433747648508E-4 0.0 26-27 0.0 0.0 0.0 5.548281142502053E-4 0.0 28-29 0.0 0.0 0.0 0.0014033887595740486 0.0 30-31 0.0 0.0 0.0 0.0038511598518543664 0.0 32-33 0.0 0.0 0.0 0.007180128537355598 0.0 34-35 0.0 0.0 0.0 0.01158611650346017 0.0 36-37 0.0 0.0 0.0 0.019157888415580616 0.0 38-39 0.0 0.0 0.0 0.032147393678614834 0.0 40-41 0.0 0.0 0.0 0.04487580335847249 0.0 42-43 0.0 0.0 0.0 0.0596603407558456 0.0 44-45 0.0 0.0 0.0 0.07738220346395511 0.0 46-47 0.0 0.0 0.0 0.0999343344608311 0.0 48-49 0.0 0.0 0.0 0.12659872089273802 0.0 50-51 0.0 6.52738957941418E-5 0.0 0.15473176998001315 0.0 52-53 0.0 6.52738957941418E-5 0.0 0.1832890993899502 0.0 54-55 0.0 1.305477915882836E-4 0.0 0.21749262078608048 0.0 56-57 0.0 1.305477915882836E-4 0.0 0.2630538000503915 0.0 58-59 0.0 1.305477915882836E-4 0.0 0.3147833624672488 0.0 60-61 0.0 1.305477915882836E-4 0.0 0.37408469679622663 0.0 62-63 0.0 1.305477915882836E-4 0.0 0.4386405797366329 0.0 64-65 0.0 1.305477915882836E-4 0.0 0.5037186538433922 0.0 66-67 0.0 1.305477915882836E-4 0.0 0.5803175705578176 0.0 68-69 0.0 1.305477915882836E-4 0.0 0.6713746551906454 0.0 70-71 0.0 1.305477915882836E-4 0.0 0.7739525824311393 0.0 72-73 0.0 1.305477915882836E-4 0.0 0.8803490325755905 0.0 74-75 0.0 1.305477915882836E-4 0.0 0.9919347574356758 0.0 76-77 0.0 1.305477915882836E-4 0.0 1.1184682044326197 0.0 78-79 0.0 1.305477915882836E-4 0.0 1.2662156675626597 0.0 80-81 0.0 1.305477915882836E-4 0.0 1.4278664704968518 0.0 82-83 0.0 1.305477915882836E-4 0.0 1.5905290188158534 0.0 84-85 0.0 1.305477915882836E-4 0.0 1.766768537460036 0.0 86-87 0.0 1.305477915882836E-4 0.0 1.9555080071487971 0.0 88-89 0.0 1.305477915882836E-4 0.0 2.163699097784212 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGGT 560 0.0 22.051981 4 GAGTACT 9685 0.0 21.824837 12-13 TAAGGTG 660 0.0 21.589352 5 GTACTTT 10170 0.0 20.667936 14-15 AGTACTT 9935 0.0 20.008669 12-13 GGTATCA 16170 0.0 19.596447 1 GTACATG 9455 0.0 18.741693 1 GTATCAA 19910 0.0 18.540066 1 ACTTTTT 11650 0.0 18.286356 16-17 CGCGCTT 210 1.8189894E-12 18.094818 62-63 TACATGG 9620 0.0 18.07101 2 TACTTTT 11225 0.0 17.582859 14-15 GTAAGCG 150 7.6957804E-8 17.41598 94-95 ACATGGG 9615 0.0 17.338842 3 TCGCGCT 220 5.456968E-12 17.272043 60-61 ATTCGCG 215 6.730261E-11 16.569113 80-81 CTCGCGC 230 1.0913936E-11 16.521086 60-61 GTGTAGC 900 0.0 16.363668 1 CGCGGAA 205 6.148184E-10 16.218342 82-83 CATGGGA 5390 0.0 15.949684 4 >>END_MODULE