FastQCFastQC Report
Thu 26 May 2016
SRR936422_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936422_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1500323
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98980.6597246059681815No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72860.48562876127340576No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45660.30433446664484914No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT29140.19422484358368164No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29060.19369162507006826No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25320.16876365955864173No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA20890.13923668436729958No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT19770.13177162517671195No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC18740.1249064368139394No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18610.12403995672931763No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT17220.11477528505528477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG97300.025.2536811
TACATGG99750.024.2498132
GTATCAA151250.023.8502731
GAGTACT75100.023.72588312-13
ACATGGG98000.023.6160053
GTACTTT79950.022.25540414-15
AGTACTT77650.021.87588512-13
ATGGGTA6850.021.507465
CGATCGT2100.021.48415424-25
ATGGGCG1553.891905E-521.46275
ATTCGCG2000.021.3746480-81
GTACCTG10250.021.3295141
GGTATCA113000.021.3243831
CATGGGG36300.020.946764
CATTCCG2056.8091686E-720.8609569
CGCGGAA2050.020.85052782-83
ATCAACG174500.020.4798093
TATCAAC175600.020.4327092
TCAACGC175100.020.3824944
CAACGCA175950.020.2306825
Produced by FastQC (version 0.11.5)