##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936422_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1500323 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.057906864055276 33.0 31.0 34.0 31.0 34.0 2 32.17135243544224 33.0 31.0 34.0 31.0 34.0 3 32.21189303903226 34.0 31.0 34.0 30.0 34.0 4 35.84217131910928 37.0 35.0 37.0 35.0 37.0 5 35.80179601325848 37.0 35.0 37.0 35.0 37.0 6 35.93047363800995 37.0 35.0 37.0 35.0 37.0 7 35.827839071986496 37.0 35.0 37.0 35.0 37.0 8 35.826921269619945 37.0 35.0 37.0 35.0 37.0 9 37.464106729017686 39.0 37.0 39.0 35.0 39.0 10-11 37.37191424779864 39.0 37.0 39.0 35.0 39.0 12-13 37.30680126879345 39.0 37.0 39.0 35.0 39.0 14-15 38.51318082839495 40.0 38.0 41.0 34.0 41.0 16-17 38.464853568198315 40.0 38.0 41.0 34.0 41.0 18-19 38.462048172293564 40.0 38.0 41.0 34.0 41.0 20-21 38.3903562766151 40.0 38.0 41.0 34.0 41.0 22-23 38.295734985066545 40.0 38.0 41.0 34.0 41.0 24-25 38.16554935170626 40.0 38.0 41.0 34.0 41.0 26-27 37.978793566452026 40.0 38.0 41.0 33.5 41.0 28-29 38.08507767993959 40.0 38.0 41.0 34.0 41.0 30-31 37.999227499678405 40.0 37.5 41.0 34.0 41.0 32-33 37.830227557665914 40.0 37.0 41.0 33.5 41.0 34-35 37.66330650133338 40.0 37.0 41.0 33.0 41.0 36-37 37.446055282762444 40.0 37.0 41.0 33.0 41.0 38-39 37.268474521819634 39.5 36.0 41.0 32.5 41.0 40-41 37.10113755504648 39.0 36.0 41.0 32.0 41.0 42-43 36.83081309824618 39.0 36.0 41.0 31.0 41.0 44-45 36.53326517023335 39.0 35.5 41.0 30.5 41.0 46-47 36.12380367427548 39.0 35.0 40.0 30.0 41.0 48-49 35.80747978935203 39.0 35.0 40.0 29.0 41.0 50-51 35.09355485452133 38.0 34.5 40.0 27.5 40.5 52-53 35.26368988544466 38.5 35.0 40.0 28.0 40.5 54-55 35.614678639199695 39.0 35.0 40.5 28.0 41.0 56-57 35.54606008172907 39.0 35.0 41.0 28.0 41.0 58-59 35.33543243688193 39.0 35.0 40.0 28.0 41.0 60-61 35.039996387444575 38.0 35.0 40.0 27.0 41.0 62-63 34.74006330636803 38.0 34.5 40.0 26.0 41.0 64-65 34.43734215898843 37.0 34.0 40.0 26.0 41.0 66-67 34.08003276627766 37.0 34.0 39.5 26.0 41.0 68-69 33.70901232601247 36.0 34.0 39.0 25.5 41.0 70-71 33.309053450490325 36.0 33.5 39.0 24.5 40.5 72-73 32.90853369574418 35.0 33.0 38.5 24.0 40.0 74-75 32.495127715831856 35.0 33.0 37.5 22.5 39.5 76-77 32.08657902331698 35.0 32.5 37.0 21.5 39.0 78-79 31.65203259564774 35.0 32.0 36.5 20.0 39.0 80-81 31.25752387985787 35.0 31.5 36.0 18.5 37.5 82-83 30.878050926367187 35.0 31.0 35.5 17.5 37.0 84-85 30.51211205853673 34.0 31.0 35.0 14.0 37.0 86-87 30.204760908151112 34.0 31.0 35.0 8.5 36.0 88-89 29.851338345143013 34.0 30.5 35.0 6.0 36.0 90-91 29.537829853971445 34.0 30.0 35.0 2.0 36.0 92-93 29.226842486584555 34.0 30.0 35.0 2.0 35.0 94-95 28.873455249302985 34.0 29.5 35.0 2.0 35.0 96-97 28.386351472316296 34.0 29.0 35.0 2.0 35.0 98-99 27.83072211783729 34.0 29.0 35.0 2.0 35.0 100-101 25.743326270409774 32.0 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1336327840857443 1101 2 0.26681574695106747 1101 3 0.23750003513951867 1101 4 0.20944785946806377 1101 5 0.27100261231915823 1101 6 0.1425419909251815 1101 7 0.25007283213118114 1101 8 0.2629058280831984 1101 9 0.39975056446061785 1101 10-11 0.3266785717878591 1101 12-13 0.4034906907869953 1101 14-15 0.43624032336360585 1101 16-17 0.49268368440578314 1101 18-19 0.4769937739314045 1101 20-21 0.41155612471809633 1101 22-23 0.3539571379722659 1101 24-25 0.4294360386403042 1101 26-27 0.4239899949345656 1101 28-29 0.3791538616351957 1101 30-31 0.3400047292294488 1101 32-33 0.29816967011832674 1101 34-35 0.292950003455843 1101 36-37 0.3863691395408537 1101 38-39 0.2327398079170706 1101 40-41 0.22334782074779724 1101 42-43 0.07938521017565137 1101 44-45 0.09427742450296961 1101 46-47 0.19861394514001773 1101 48-49 0.2841026533017583 1101 50-51 0.3202245253795155 1101 52-53 0.2987133487170439 1101 54-55 0.2974858876902644 1101 56-57 0.2675146295970876 1101 58-59 0.3765267356480564 1101 60-61 0.3225844447704205 1101 62-63 0.33841273214871137 1101 64-65 0.4572815064370843 1101 66-67 0.4300242936100531 1101 68-69 0.41669293779653316 1101 70-71 0.34679611576839875 1101 72-73 0.3606164834921941 1101 74-75 0.248543674984532 1101 76-77 0.28173893009400786 1101 78-79 0.19022326879596463 1101 80-81 0.2203428477153473 1101 82-83 0.1881084352629543 1101 84-85 0.1542502821996976 1101 86-87 0.21725812122230437 1101 88-89 0.14440123579042563 1101 90-91 0.11423104039893062 1101 92-93 0.05019327974373766 1101 94-95 0.031753497566011646 1101 96-97 -0.06471159533564119 1101 98-99 -0.03533483792261194 1101 100-101 0.2486593173746705 1102 1 0.046135502531253536 1102 2 0.05042341086784319 1102 3 0.0737719009990414 1102 4 0.12652728069456032 1102 5 0.16715548704002714 1102 6 0.03982830857373898 1102 7 -0.04617881538781177 1102 8 0.06770953281520775 1102 9 0.26248426459869023 1102 10-11 0.24905167600507383 1102 12-13 0.28832276762922504 1102 14-15 0.1444364287321207 1102 16-17 0.09679544682963126 1102 18-19 0.08735310385574024 1102 20-21 0.12878828027773181 1102 22-23 0.024485772996122535 1102 24-25 0.001643278510918833 1102 26-27 -0.07024486371567207 1102 28-29 0.14680965598220297 1102 30-31 0.06730923461024929 1102 32-33 0.12367649177137707 1102 34-35 0.09442858711909707 1102 36-37 -0.0824243595043157 1102 38-39 -0.036757165285798976 1102 40-41 -0.19037561291053606 1102 42-43 -0.006192864519391605 1102 44-45 0.17204580479830156 1102 46-47 0.25057949875340313 1102 48-49 0.48933228438912124 1102 50-51 0.4591997638663159 1102 52-53 0.3122010688641197 1102 54-55 0.3069973133088908 1102 56-57 0.5321027326141063 1102 58-59 0.4275042479147473 1102 60-61 0.1021420165890845 1102 62-63 0.3414702391007225 1102 64-65 0.4721461544869143 1102 66-67 0.42621972336260683 1102 68-69 0.47527300988312504 1102 70-71 0.4777701935494676 1102 72-73 0.4391761116151187 1102 74-75 0.3401394636032222 1102 76-77 0.44159480704416154 1102 78-79 0.4179917117643903 1102 80-81 0.3984647982775549 1102 82-83 0.3516914811879204 1102 84-85 0.2843497569364857 1102 86-87 0.14410686785440774 1102 88-89 -0.1417942002294339 1102 90-91 -0.26861116566895404 1102 92-93 -0.20489201520524958 1102 94-95 -0.06582553477393205 1102 96-97 -0.17099968099221385 1102 98-99 -0.3771681575723207 1102 100-101 0.04864565463990189 1103 1 0.17780039450476792 1103 2 0.24473194174154855 1103 3 0.20239244737054918 1103 4 0.12054638721438238 1103 5 0.31743522840069716 1103 6 0.09554312342801552 1103 7 0.24271156372800817 1103 8 0.028477742012988472 1103 9 0.2785727615161093 1103 10-11 0.2575958990811813 1103 12-13 0.2961860475253886 1103 14-15 0.34762197681774154 1103 16-17 0.38537904114418353 1103 18-19 0.2422825621533704 1103 20-21 0.25810199108276066 1103 22-23 0.2639231628873233 1103 24-25 0.26324124815332794 1103 26-27 0.3979424298156502 1103 28-29 0.4507846657121988 1103 30-31 0.3298122037481406 1103 32-33 0.3126090812168556 1103 34-35 0.27143244966195823 1103 36-37 0.32209960386702363 1103 38-39 0.32079190304730787 1103 40-41 0.3187611842812075 1103 42-43 0.4423523675935499 1103 44-45 0.550959191207383 1103 46-47 0.4947634355137396 1103 48-49 0.32232462385668725 1103 50-51 -0.12343345876544021 1103 52-53 0.13903044950979648 1103 54-55 0.2802152195135861 1103 56-57 0.2615689897329787 1103 58-59 0.2828121263615273 1103 60-61 0.3455176271033764 1103 62-63 0.34690650338313134 1103 64-65 0.37375942263187056 1103 66-67 0.31847276473123287 1103 68-69 0.1298865957806825 1103 70-71 0.1675775427219648 1103 72-73 0.1678078764706754 1103 74-75 0.22617674406720312 1103 76-77 0.040798952744069084 1103 78-79 0.10811681352982916 1103 80-81 0.25998044680934385 1103 82-83 0.2019815949458561 1103 84-85 0.09111324935711451 1103 86-87 0.1490248256390636 1103 88-89 0.08069795153221548 1103 90-91 0.2119094095948526 1103 92-93 0.02754322311859525 1103 94-95 -0.16133823516331702 1103 96-97 0.04315929934159968 1103 98-99 -0.018064169745940717 1103 100-101 0.088693292459606 1104 1 0.24428342861502372 1104 2 0.2689331204368486 1104 3 0.28180477944714966 1104 4 0.1792285371063116 1104 5 0.1896933708073547 1104 6 0.21414622121038462 1104 7 0.3487652703454742 1104 8 0.27142387660891387 1104 9 0.4561139049634093 1104 10-11 0.436856793351609 1104 12-13 0.3568487650860064 1104 14-15 0.3692884750743701 1104 16-17 0.18069490182664794 1104 18-19 0.2746399265056496 1104 20-21 0.30856824630402 1104 22-23 0.32398780609644007 1104 24-25 0.22520463799806834 1104 26-27 0.3033430116832676 1104 28-29 0.2172023479788905 1104 30-31 0.2386132467530615 1104 32-33 0.07553969146921702 1104 34-35 0.2441854551249989 1104 36-37 0.15482283356038096 1104 38-39 0.3034480749252779 1104 40-41 -0.031380017694047524 1104 42-43 0.10157924741045576 1104 44-45 0.06351973680341416 1104 46-47 0.28919317506434083 1104 48-49 0.2847597805239488 1104 50-51 -0.029987868112065996 1104 52-53 -0.03898967833310962 1104 54-55 0.20505469412676547 1104 56-57 0.15084945018789853 1104 58-59 0.3233492975190586 1104 60-61 0.24062529360951146 1104 62-63 0.13502414719984301 1104 64-65 0.23758678353283358 1104 66-67 0.3201833295522576 1104 68-69 0.32548151408430925 1104 70-71 0.3729978744941178 1104 72-73 0.09458067645598334 1104 74-75 0.008561994497306102 1104 76-77 0.013963687944432479 1104 78-79 0.008093389224452352 1104 80-81 0.30592554863617494 1104 82-83 0.14545684326317598 1104 84-85 0.11067582505961582 1104 86-87 0.1235884577318096 1104 88-89 0.10020213050702864 1104 90-91 0.005426454221762356 1104 92-93 0.13375834091594996 1104 94-95 0.08475935429850168 1104 96-97 0.23412545679754615 1104 98-99 0.4513142922286022 1104 100-101 0.6582838198542298 1105 1 0.03997929295085001 1105 2 0.1787474637698523 1105 3 0.1553010767249674 1105 4 0.09030776873568414 1105 5 0.25732602182662845 1105 6 0.19141338644089956 1105 7 0.31725258292770064 1105 8 0.051069653801981474 1105 9 0.18993029807798223 1105 10-11 0.2417415201803621 1105 12-13 0.1408564125560261 1105 14-15 0.2504897065658298 1105 16-17 0.0809914846676989 1105 18-19 0.06325890645538124 1105 20-21 -0.08735531144614583 1105 22-23 -0.1788580235580497 1105 24-25 -0.17220014383404703 1105 26-27 -0.2177297839564858 1105 28-29 -0.11474159130647621 1105 30-31 -0.23546330366466606 1105 32-33 -0.13149027509337685 1105 34-35 -0.3703626532213633 1105 36-37 -0.36978231982845244 1105 38-39 -0.318715729123177 1105 40-41 -0.4272112947106663 1105 42-43 -0.43385925846643403 1105 44-45 -0.3455499381926259 1105 46-47 -0.2582462204934757 1105 48-49 -0.386361487852767 1105 50-51 -0.26344325088443554 1105 52-53 -0.12330418459400505 1105 54-55 -0.2916897903247744 1105 56-57 -0.1641209185699637 1105 58-59 -0.4576445220379668 1105 60-61 -0.3669429434296205 1105 62-63 -0.4015308541454985 1105 64-65 -0.36984860981294787 1105 66-67 -0.6347721796413381 1105 68-69 -0.3516910718449324 1105 70-71 -0.16458573590676906 1105 72-73 -0.36548103332685855 1105 74-75 -0.2516727163090735 1105 76-77 -0.14815260335711145 1105 78-79 -0.21030941719152452 1105 80-81 -0.33232315909553023 1105 82-83 -0.24237677017993065 1105 84-85 -0.43342321075883206 1105 86-87 -0.24474654093637227 1105 88-89 -0.13387994999422048 1105 90-91 -0.11458210456275353 1105 92-93 -0.022033508903749777 1105 94-95 0.11491250651371132 1105 96-97 -0.051274852292205964 1105 98-99 -0.15593383545277817 1105 100-101 0.3813696199788943 1106 1 -0.054899490891088476 1106 2 -0.08400797454936537 1106 3 -0.0398653422249069 1106 4 0.010183884768970586 1106 5 0.1444097129020605 1106 6 0.07880570636032758 1106 7 0.18042120029139852 1106 8 0.2070974018018248 1106 9 0.23203285212987623 1106 10-11 0.15554270461765185 1106 12-13 0.29646934246554224 1106 14-15 0.16183092731321835 1106 16-17 0.07082451820676994 1106 18-19 0.12130885341306197 1106 20-21 0.11617494917609861 1106 22-23 0.11702322861717107 1106 24-25 0.04618266194739107 1106 26-27 0.04274354458293317 1106 28-29 -0.08121124698828197 1106 30-31 -0.042895121101466316 1106 32-33 -0.14542146725162297 1106 34-35 -0.2576714515496832 1106 36-37 -0.29081779924238305 1106 38-39 -0.3678063725428231 1106 40-41 -0.23867254941071536 1106 42-43 -0.2416317881976866 1106 44-45 -0.2137868688944664 1106 46-47 -0.10919243816852742 1106 48-49 -0.10114868041833347 1106 50-51 0.07692815690249688 1106 52-53 -0.06244048415857861 1106 54-55 0.14444688098934222 1106 56-57 0.12380032492877291 1106 58-59 0.01855548619213465 1106 60-61 0.0404502692466977 1106 62-63 0.07135461979332547 1106 64-65 0.07858369515280827 1106 66-67 0.038699549607812855 1106 68-69 -0.04497136751485442 1106 70-71 0.08338986811649818 1106 72-73 0.17233890318975398 1106 74-75 0.08513394114639539 1106 76-77 -0.030391170314921112 1106 78-79 0.11784395007121873 1106 80-81 0.13837279371668743 1106 82-83 0.19471560435111002 1106 84-85 0.14506790456652396 1106 86-87 0.31905628254675733 1106 88-89 0.22161653791415503 1106 90-91 0.21411755319052617 1106 92-93 0.1734105016391574 1106 94-95 0.15091658948151476 1106 96-97 0.12093882209285667 1106 98-99 0.2739814363393833 1106 100-101 0.5074101525362771 1107 1 0.3128779699185422 1107 2 0.3000872241564423 1107 3 0.34802139164303014 1107 4 0.2952794180433216 1107 5 0.3643963229107712 1107 6 0.2594143601224843 1107 7 0.40536616684723725 1107 8 0.4242483349870767 1107 9 0.46741468980649614 1107 10-11 0.5184252427858311 1107 12-13 0.5000042784172365 1107 14-15 0.5658811640666812 1107 16-17 0.604529670888553 1107 18-19 0.70026938556191 1107 20-21 0.7866762256475184 1107 22-23 0.6954206489233385 1107 24-25 0.8268981746546515 1107 26-27 0.7037551087651721 1107 28-29 0.5679302022843515 1107 30-31 0.5887661186435409 1107 32-33 0.63050224822085 1107 34-35 0.6112093967392553 1107 36-37 0.6139476094876457 1107 38-39 0.6728924588596428 1107 40-41 0.5964489555170118 1107 42-43 0.6797021942155368 1107 44-45 0.5297266517706731 1107 46-47 0.7078581969498572 1107 48-49 0.4950517373755048 1107 50-51 0.4182143500235753 1107 52-53 0.38353104597960197 1107 54-55 0.4829582938364112 1107 56-57 0.5356453200117386 1107 58-59 0.6103918325151554 1107 60-61 0.5389050193044227 1107 62-63 0.6245149513460362 1107 64-65 0.5118131660379106 1107 66-67 0.4156897883984101 1107 68-69 0.37908048589699916 1107 70-71 0.6105049629574353 1107 72-73 0.5763866025869859 1107 74-75 0.3596961837633188 1107 76-77 0.5067227392181763 1107 78-79 0.6775463109361972 1107 80-81 0.6271915710227205 1107 82-83 0.7351574378157224 1107 84-85 0.7115651241678194 1107 86-87 0.5149561323715197 1107 88-89 0.7340365341661155 1107 90-91 0.6314124269697388 1107 92-93 0.5077932734253494 1107 94-95 0.6493718832584285 1107 96-97 0.5238686348323434 1107 98-99 0.6688252385024427 1107 100-101 0.781889931075824 1108 1 0.08432628985177004 1108 2 -0.03693032313517364 1108 3 -0.03919275573856851 1108 4 0.13624526896632716 1108 5 0.19916747779773658 1108 6 0.2524241347007532 1108 7 0.315197475493072 1108 8 0.18399846051117663 1108 9 0.3087065773629831 1108 10-11 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-3.1867347435853546 2316 100-101 -3.626914624578294 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2133.0 3 625.0 4 598.0 5 632.0 6 718.0 7 833.0 8 1007.0 9 1269.0 10 1718.0 11 2483.0 12 3582.0 13 5013.0 14 6084.0 15 8320.0 16 11508.0 17 17101.0 18 14514.0 19 7185.0 20 6435.0 21 6378.0 22 6720.0 23 7403.0 24 8311.0 25 9768.0 26 11447.0 27 13618.0 28 16879.0 29 21321.0 30 27412.0 31 36092.0 32 48840.0 33 68953.0 34 100667.0 35 152230.0 36 230067.0 37 322597.0 38 273206.0 39 46634.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.055103125047914 19.126178450561238 14.818463786413396 22.000254637977452 2 15.120221085215926 20.616156391394423 40.6237418646133 23.639880658776352 3 19.44388711097661 25.811842295588782 30.044080818611395 24.700189774823205 4 12.31385502043886 16.7938025348054 38.12489207174903 32.7674503730067 5 13.129351767512759 38.29655432699553 33.84039773871458 14.733696166777136 6 30.350875438052462 36.5775390371015 18.014984645240023 15.05660087960601 7 27.575909905489297 34.02371834531607 20.75255210888998 17.64781964030465 8 26.561128114939507 33.332777773703675 20.045747002144683 20.060347109212135 9 26.851820990082114 16.94806675208217 20.95288503971021 35.24722721812551 10-11 24.606983187141815 26.76478334650224 27.09743989811601 21.530793568239933 12-13 26.295717934852547 24.586219059360552 27.72547335686143 21.392589648925465 14-15 23.202567322896584 25.411376962890074 27.23800800488506 24.148047709328278 16-17 21.39557720229913 29.118707026317857 27.55112176559919 21.934594005783822 18-19 21.117305138968938 27.167288421427386 30.804345427410702 20.91106101219297 20-21 22.11449518311689 25.989535225197326 30.63561656803787 21.26035302364791 22-23 21.666138939902478 26.13514026977392 30.54098103849961 21.65773975182399 24-25 21.63909305352909 26.368388181727898 30.72152194880046 21.27099681594255 26-27 21.37161828668857 26.35407671898865 30.809804881511514 21.464500112811262 28-29 20.91098688468427 26.795649157139174 31.010253234837936 21.283110723338623 30-31 21.008386277340055 26.46661536405469 30.921110735988073 21.603887622617187 32-33 20.834669171042993 26.666708880249175 30.747040439056804 21.751581509651025 34-35 21.695050933044858 26.535339861348007 30.307511216067688 21.462097989539448 36-37 21.653925397418952 26.174826344798806 30.59347719002855 21.57777106775369 38-39 21.37908900152242 26.483958609399536 30.58775772474344 21.5491946643346 40-41 21.399560012970987 26.582621878773278 30.75793281416064 21.259885294095103 42-43 21.200000533221 26.76102900988858 30.19500558548999 21.843964871400427 44-45 21.301178623870147 26.960179536348843 29.83715354636945 21.901488293411557 46-47 21.699484331138528 27.068166071168502 29.30787460644964 21.92447499124333 48-49 21.794986727809277 27.22423753638813 28.923744474086316 22.05703126171627 50-51 21.666546691141676 27.55927790730691 28.960225447342076 21.813949954209342 52-53 21.89200997524186 27.68896121446537 28.648460039545043 21.770568770747733 54-55 21.33448510758558 27.353572033214824 29.068135978681514 22.243806880518086 56-57 21.753860807311064 27.820774690953787 28.466411584604828 21.958952917130322 58-59 21.879523067289078 27.290457113282017 28.596533149636187 22.23348666979272 60-61 21.679602296453375 27.310492107149603 29.19113238666452 21.818773209732502 62-63 21.711346321760168 27.386073544800745 29.135378688404394 21.767201445034694 64-65 21.388209847844816 27.68427840849443 28.932880750394514 21.994630993266245 66-67 21.343349639102385 27.81237856490125 28.513277792848967 22.330994003147396 68-69 21.43006168420407 27.615746202895302 28.87485985407493 22.079332258825694 70-71 21.537466266311906 27.66630704063774 28.556006504598315 22.24022018845204 72-73 21.56216131186703 27.51720685688081 28.70545646995149 22.21517536130067 74-75 21.401757279003704 28.157223280547424 28.382548787754768 22.058470652694105 76-77 21.548701145050632 27.70254012076388 28.467795742536573 22.28096299164891 78-79 21.521918422889648 27.80768433257304 28.505617535538384 22.164779708998925 80-81 21.619294602351324 28.187906040313198 28.162739457535142 22.03005989980033 82-83 21.567455768020732 28.092339255814945 28.254809794490598 22.08539518167372 84-85 21.47045874262639 28.04492205531468 28.095382044442786 22.389237157616144 86-87 21.644323038733397 28.00349152515258 28.22152853878539 22.13065689732863 88-89 21.672522384707012 27.924309595178915 27.930541918875807 22.472626101238266 90-91 21.434782594204624 28.001601142797337 28.231040506179767 22.332575756818272 92-93 21.694680471307862 28.026625807808493 28.235841915682276 22.042851805201373 94-95 21.4161686924469 27.739102613239837 28.24265369989568 22.602074994417578 96-97 21.458025680069934 27.574464170913405 28.28627009098705 22.681240058029612 98-99 21.318252454498364 27.864381423745716 27.855448096367937 22.961918025387984 100-101 21.404005118251206 27.65996035288995 28.18318751829495 22.752847010563894 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 535.0 1 426.0 2 487.5 3 672.0 4 547.0 5 476.0 6 851.0 7 1684.5 8 2765.5 9 3262.0 10 2800.5 11 2293.5 12 2240.0 13 2379.5 14 2568.0 15 2686.5 16 2738.0 17 2884.0 18 3073.0 19 3311.0 20 3624.0 21 4093.0 22 4790.5 23 5720.5 24 6839.0 25 8167.0 26 10224.0 27 12676.0 28 14655.0 29 17011.0 30 20124.0 31 23643.5 32 27910.0 33 31060.0 34 34129.0 35 36736.0 36 39955.0 37 43475.5 38 43611.5 39 42820.5 40 43062.5 41 44932.5 42 46123.0 43 47498.5 44 49701.5 45 51498.0 46 51591.0 47 54054.5 48 67543.5 49 67045.5 50 53810.0 51 48852.0 52 44837.5 53 43419.0 54 42851.0 55 40131.5 56 36699.0 57 32728.0 58 29775.0 59 26837.5 60 22516.5 61 18654.0 62 15531.5 63 11992.5 64 8877.5 65 7148.5 66 5496.0 67 4265.0 68 3117.5 69 2045.5 70 1595.0 71 1335.0 72 1119.0 73 1001.5 74 804.0 75 623.5 76 485.0 77 320.5 78 225.5 79 164.5 80 108.0 81 72.5 82 50.0 83 35.0 84 19.5 85 8.5 86 6.5 87 6.0 88 6.0 89 5.5 90 2.5 91 3.0 92 2.5 93 2.0 94 2.5 95 1.5 96 1.0 97 1.0 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010264456387057986 2 0.005798751335545746 3 0.008264886961007729 4 0.03225972007361082 5 0.04299074266008053 6 0.05252202359092009 7 0.025794445596048314 8 0.022261872943359528 9 0.013663724411343425 10-11 0.03852503760856829 12-13 0.03705868669613143 14-15 0.015929903094200382 16-17 0.018462691033864043 18-19 0.02676090415197261 20-21 0.02256180835726707 22-23 0.011864111927898192 24-25 0.0023994833112603088 26-27 0.0019662432689494195 28-29 0.0010664370272268039 30-31 0.004965597408024805 32-33 0.0010664370272268039 34-35 0.0013330462840335047 36-37 0.005332185136134019 38-39 0.005265532821932344 40-41 0.0034325941813862747 42-43 4.665661994117267E-4 44-45 9.331323988234534E-4 46-47 0.0025661140967644966 48-49 3.665877281092138E-4 50-51 0.0011330893414284791 52-53 5.665446707142396E-4 54-55 0.001932917111848582 56-57 0.0014330247553360177 58-59 0.0018329386405460692 60-61 6.331969849159148E-4 62-63 8.664800846217782E-4 64-65 0.003032680296176223 66-67 0.0023994833112603088 68-69 0.006731883734369199 70-71 0.010231130229957149 72-73 0.0032659633958820864 74-75 0.0032659633958820864 76-77 0.0026660925680670093 78-79 0.03105997841798066 80-81 0.021195435916132727 82-83 0.003399268024285437 84-85 0.008298213118108567 86-87 0.007931625389999353 88-89 0.005332185136134019 90-91 0.009631259402142073 92-93 0.01329713668323421 94-95 0.005198880507730669 96-97 0.003632551123991301 98-99 0.021462045172939426 100-101 0.03945817000739174 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1500323.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.04438474287103 #Duplication Level Percentage of deduplicated Percentage of total 1 76.98126824643887 40.83424010858265 2 13.24126022750692 14.047490039767062 3 4.231851715952371 6.734279117872696 4 1.8349003208778432 3.8932463434184736 5 0.9770369152404877 2.5913161020002162 6 0.5599249305946679 1.7820524067353354 7 0.3848810042169515 1.429104324753458 8 0.2549332111725982 1.081822026973991 9 0.18950246054373515 0.9046837284122364 >10 1.1575035438289412 11.717750685525843 >50 0.11815760063676299 4.3548726302584555 >100 0.06260735093507055 6.146630917213631 >500 0.004282939793177321 1.518604144311335 >1k 0.0016375946268030934 1.8156831263858804 >5k 2.519376348927836E-4 1.1482242977887964 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9898 0.6597246059681815 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7286 0.48562876127340576 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4566 0.30433446664484914 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2914 0.19422484358368164 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2906 0.19369162507006826 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2532 0.16876365955864173 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2089 0.13923668436729958 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1977 0.13177162517671195 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1874 0.1249064368139394 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1861 0.12403995672931763 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1722 0.11477528505528477 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 6.665231420167524E-5 0.0 5 0.0 0.0 0.0 6.665231420167524E-5 0.0 6 0.0 0.0 0.0 6.665231420167524E-5 0.0 7 0.0 0.0 0.0 6.665231420167524E-5 0.0 8 0.0 0.0 0.0 6.665231420167524E-5 0.0 9 0.0 0.0 0.0 6.665231420167524E-5 0.0 10-11 0.0 0.0 0.0 1.3330462840335048E-4 0.0 12-13 0.0 0.0 0.0 1.999569426050257E-4 0.0 14-15 0.0 0.0 0.0 1.999569426050257E-4 0.0 16-17 0.0 0.0 0.0 1.999569426050257E-4 0.0 18-19 0.0 0.0 0.0 1.999569426050257E-4 0.0 20-21 0.0 0.0 0.0 1.999569426050257E-4 0.0 22-23 0.0 0.0 0.0 2.3328309970586334E-4 0.0 24-25 0.0 0.0 0.0 3.3326157100837617E-4 0.0 26-27 0.0 0.0 0.0 5.332185136134019E-4 0.0 28-29 0.0 0.0 0.0 0.0011330893414284791 0.0 30-31 0.0 0.0 0.0 0.0023328309970586333 0.0 32-33 0.0 0.0 0.0 0.004765640465419779 0.0 34-35 0.0 0.0 0.0 0.008798105474621131 0.0 36-37 0.0 0.0 0.0 0.015296706109284467 0.0 38-39 0.0 0.0 0.0 0.02659427336646842 0.0 40-41 0.0 0.0 0.0 0.04049128087751771 0.0 42-43 0.0 0.0 0.0 0.05488818074507956 0.0 44-45 0.0 0.0 0.0 0.07018488685436403 0.0 46-47 0.0 0.0 0.0 0.0896473626012532 0.0 48-49 0.0 0.0 0.0 0.11354221724255377 0.0 50-51 0.0 0.0 0.0 0.13870346585368618 0.0 52-53 0.0 0.0 0.0 0.16639750240448223 0.0 54-55 0.0 0.0 0.0 0.1993237456201098 0.0 56-57 0.0 0.0 0.0 0.24088146352485432 0.0 58-59 0.0 0.0 0.0 0.29067074223350575 0.0 60-61 0.0 0.0 0.0 0.34805838476114814 0.0 62-63 0.0 0.0 0.0 0.40681239972992483 0.0 64-65 0.0 0.0 0.0 0.4681658549525669 0.0 66-67 0.0 0.0 0.0 0.5435496223146615 0.0 68-69 0.0 0.0 0.0 0.6341634434718391 0.0 70-71 0.0 0.0 0.0 0.7373078996989315 0.0 72-73 0.0 0.0 0.0 0.8454512794911495 0.0 74-75 0.0 0.0 0.0 0.9571938842502581 6.665231420167524E-5 76-77 0.0 0.0 0.0 1.0812005148224748 6.665231420167524E-5 78-79 0.0 0.0 0.0 1.2264692336250262 6.665231420167524E-5 80-81 0.0 0.0 0.0 1.3928667360295082 6.665231420167524E-5 82-83 0.0 0.0 0.0 1.5642298358420152 6.665231420167524E-5 84-85 0.0 0.0 0.0 1.7444910196004462 6.665231420167524E-5 86-87 0.0 0.0 0.0 1.9405821279817745 6.665231420167524E-5 88-89 0.0 0.0 0.0 2.164400599071 6.665231420167524E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9730 0.0 25.253681 1 TACATGG 9975 0.0 24.249813 2 GTATCAA 15125 0.0 23.850273 1 GAGTACT 7510 0.0 23.725883 12-13 ACATGGG 9800 0.0 23.616005 3 GTACTTT 7995 0.0 22.255404 14-15 AGTACTT 7765 0.0 21.875885 12-13 ATGGGTA 685 0.0 21.50746 5 CGATCGT 210 0.0 21.484154 24-25 ATGGGCG 155 3.891905E-5 21.4627 5 ATTCGCG 200 0.0 21.37464 80-81 GTACCTG 1025 0.0 21.329514 1 GGTATCA 11300 0.0 21.324383 1 CATGGGG 3630 0.0 20.94676 4 CATTCCG 205 6.8091686E-7 20.860956 9 CGCGGAA 205 0.0 20.850527 82-83 ATCAACG 17450 0.0 20.479809 3 TATCAAC 17560 0.0 20.432709 2 TCAACGC 17510 0.0 20.382494 4 CAACGCA 17595 0.0 20.230682 5 >>END_MODULE