FastQCFastQC Report
Thu 26 May 2016
SRR936422_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936422_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1500323
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91950.6128680290844039No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85180.5677444123698696No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52800.35192421898484527No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT49800.3319285247243427No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA25230.16816378873082663No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23350.15563315366091168No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA20960.13970325056671132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGGT6400.023.747364
TAAGGTG7650.022.969775
ATACGTC1703.1549152E-622.3489655
GGTAAGG7350.021.9710793
TAGCGCA1302.9443012E-421.9206374
AGTACTT99250.021.48448412-13
GAGTACT98650.021.47073612-13
AGGTAAG7450.021.0407352
GTACTTT103300.020.5498714-15
GTACATG92200.020.3934971
GCACCGT3353.45608E-1119.8478786
TACATGG95550.019.338472
TACTTTT113400.018.78240814-15
ACATGGG95300.018.689523
GGTATCA161950.018.0373571
GTATCAA197200.017.9752161
ACTTTTT120450.017.88079616-17
TGCACCG3351.4611942E-817.01195
TATAAGC7350.016.8014133
AAGGTAA9250.016.5937611
Produced by FastQC (version 0.11.5)