##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936422_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1500323 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.812097128418348 31.0 31.0 33.0 28.0 34.0 2 31.01950846584369 31.0 31.0 34.0 28.0 34.0 3 31.238527970310393 31.0 31.0 34.0 28.0 34.0 4 35.150508923745086 35.0 35.0 37.0 32.0 37.0 5 34.90433926561147 35.0 35.0 37.0 32.0 37.0 6 35.07295095789373 36.0 35.0 37.0 32.0 37.0 7 34.99543564952347 36.0 35.0 37.0 32.0 37.0 8 34.97131484353702 36.0 35.0 37.0 32.0 37.0 9 36.45067428813662 38.0 35.0 39.0 32.0 39.0 10-11 36.29521876289306 38.0 35.0 39.0 32.0 39.0 12-13 36.13597305380242 38.0 35.0 39.0 31.0 39.0 14-15 36.97608081726402 38.0 36.0 40.0 31.0 41.0 16-17 36.85792992575598 38.0 36.0 40.0 31.0 41.0 18-19 36.962342108999195 38.5 36.0 40.0 31.0 41.0 20-21 37.09207550640762 39.0 36.0 40.0 31.0 41.0 22-23 36.98032823598652 39.0 36.0 40.0 31.0 41.0 24-25 36.8174486427256 39.0 35.5 40.0 30.5 41.0 26-27 36.49592421098657 38.0 35.0 40.0 30.0 41.0 28-29 36.335682716321756 38.0 35.0 40.0 30.0 41.0 30-31 36.23352271477542 38.0 35.0 40.0 30.0 41.0 32-33 35.96093674495425 38.0 34.5 40.0 29.5 41.0 34-35 35.711406477138596 38.0 34.0 40.0 29.0 41.0 36-37 35.39139005400837 38.0 34.0 40.0 27.0 41.0 38-39 35.149822738170386 38.0 34.0 40.0 27.0 41.0 40-41 34.81238206706156 38.0 33.0 40.0 25.5 41.0 42-43 34.45266119362297 38.0 33.0 40.0 24.5 41.0 44-45 34.029245035902264 37.0 33.0 40.0 24.0 41.0 46-47 33.44180653099366 37.0 32.5 40.0 22.0 41.0 48-49 32.81799252560948 37.0 31.0 40.0 18.0 41.0 50-51 32.52958929510512 37.0 31.0 39.0 16.0 40.0 52-53 32.15482832696692 36.0 31.0 39.0 13.5 40.0 54-55 31.79142224707613 36.0 30.0 39.0 10.5 40.0 56-57 31.447411657356447 35.5 30.0 39.0 8.5 40.0 58-59 30.99400495759913 35.0 29.0 38.5 8.0 40.0 60-61 30.524828320301694 35.0 28.0 38.0 7.0 40.0 62-63 30.127634849295784 34.0 28.0 38.0 4.0 40.0 64-65 29.581766392970046 34.0 27.5 38.0 2.0 40.0 66-67 29.077112728392486 34.0 26.0 37.0 2.0 39.0 68-69 28.543590613487893 33.0 26.0 36.5 2.0 39.0 70-71 27.950348691581745 32.5 25.5 36.0 2.0 39.0 72-73 27.414702034161976 32.0 25.0 35.5 2.0 38.0 74-75 26.9369229159321 32.0 24.0 35.0 2.0 37.5 76-77 25.865712583223747 30.5 23.0 34.0 2.0 36.0 78-79 26.60023774880476 32.0 24.0 35.0 2.0 36.0 80-81 26.362406628439345 32.0 24.0 35.0 2.0 36.0 82-83 25.901176613302603 31.5 22.5 34.5 2.0 36.0 84-85 25.270520747865625 31.0 20.0 34.0 2.0 35.5 86-87 24.698278304071856 31.0 18.5 34.0 2.0 35.0 88-89 24.0561742371476 30.5 15.0 34.0 2.0 35.0 90-91 23.341558451080203 30.0 7.0 34.0 2.0 35.0 92-93 22.58500869479439 29.0 2.0 34.0 2.0 35.0 94-95 21.716952949464883 29.0 2.0 34.0 2.0 35.0 96-97 20.64321849361771 29.0 2.0 33.5 2.0 34.5 98-99 19.340005118897732 26.5 2.0 33.0 2.0 34.0 100-101 16.50327929385872 21.0 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.44197515550603583 1101 2 0.479859134541865 1101 3 0.31459811557858686 1101 4 0.22514901644497343 1101 5 0.2953793696399458 1101 6 0.40706491764171915 1101 7 0.37042356028927514 1101 8 0.3356757524366287 1101 9 0.23852297682812917 1101 10-11 0.31495893958636145 1101 12-13 0.3637036575970143 1101 14-15 0.5260174095596284 1101 16-17 0.5004486885072339 1101 18-19 0.4995831494560079 1101 20-21 0.13791760093599947 1101 22-23 0.3126797564403532 1101 24-25 0.19784976480596583 1101 26-27 0.2432324617423447 1101 28-29 0.3698310255095194 1101 30-31 0.33268783428218995 1101 32-33 0.5896232131726222 1101 34-35 0.7998873251366092 1101 36-37 0.8476418856545749 1101 38-39 0.7804794310956567 1101 40-41 0.6900256570616321 1101 42-43 0.8729253707504085 1101 44-45 0.8306714786639091 1101 46-47 0.9816659823618465 1101 48-49 1.1386854810385572 1101 50-51 1.1485288069342587 1101 52-53 1.23865854490883 1101 54-55 1.1883565939253948 1101 56-57 1.2306378869049759 1101 58-59 1.393261460916161 1101 60-61 1.3126420727384236 1101 62-63 1.0903042438586574 1101 64-65 1.2600460526088462 1101 66-67 1.047245027600514 1101 68-69 1.099476272497487 1101 70-71 0.8826312930378606 1101 72-73 0.8355778718512816 1101 74-75 0.8909654599957442 1101 76-77 1.2102253096586821 1101 78-79 1.234005959115546 1101 80-81 1.344927572722721 1101 82-83 1.025115288063457 1101 84-85 1.2494830449447534 1101 86-87 0.9438119723811695 1101 88-89 1.1116515114054906 1101 90-91 1.024171682980775 1101 92-93 0.9485857265531834 1101 94-95 0.9343472887980546 1101 96-97 0.8101603771002033 1101 98-99 1.0541447790496967 1101 100-101 0.927593763045838 1102 1 0.45492937092975794 1102 2 0.5472104629921297 1102 3 0.22959929696643044 1102 4 0.1592300815867631 1102 5 0.2204699792674134 1102 6 0.20972238222671535 1102 7 0.10875304653910689 1102 8 -0.010345226755276826 1102 9 0.11529357011044539 1102 10-11 0.27172515828663535 1102 12-13 0.2598156381232286 1102 14-15 0.3792020802219156 1102 16-17 0.5051510508442121 1102 18-19 0.341717613328413 1102 20-21 0.5633496222498593 1102 22-23 0.2616989538361594 1102 24-25 0.24601419519213863 1102 26-27 -0.12239266164531415 1102 28-29 0.05830907833166066 1102 30-31 0.1898835830407961 1102 32-33 0.47529307261567766 1102 34-35 0.6338703695574139 1102 36-37 0.6295620764816121 1102 38-39 0.46593460385091134 1102 40-41 0.527264412098198 1102 42-43 0.5656802355927439 1102 44-45 0.3786218804297832 1102 46-47 0.6255768837827063 1102 48-49 0.7778039275480069 1102 50-51 0.5856047059328091 1102 52-53 0.32387421243056735 1102 54-55 0.5672726439410347 1102 56-57 0.6418391985275171 1102 58-59 0.5537019211623004 1102 60-61 0.6208200969189583 1102 62-63 0.700153101422142 1102 64-65 0.6460158028126877 1102 66-67 0.8287929402417369 1102 68-69 0.941931152569385 1102 70-71 0.7863861008476825 1102 72-73 0.7581717420465246 1102 74-75 -0.07566628731135339 1102 76-77 0.360191215494865 1102 78-79 0.597878116028923 1102 80-81 0.7625903237833072 1102 82-83 0.8262950777450868 1102 84-85 1.0001967348169671 1102 86-87 0.8997792657996833 1102 88-89 0.643448392917616 1102 90-91 0.6297852494496681 1102 92-93 1.0484332115758797 1102 94-95 0.9177653979382363 1102 96-97 0.7653148004732557 1102 98-99 0.6331885568334634 1102 100-101 0.6911861472619947 1103 1 0.4969015428220054 1103 2 0.483256643035638 1103 3 0.29930932735661386 1103 4 0.21552274237929225 1103 5 0.17080405820166789 1103 6 0.06844657109585484 1103 7 0.16374179358485463 1103 8 0.3209532156302828 1103 9 0.17453656686210195 1103 10-11 0.3050495398128632 1103 12-13 0.34530048658908186 1103 14-15 0.23666293617344536 1103 16-17 0.126439628484583 1103 18-19 0.35405653563946515 1103 20-21 0.2534328897242162 1103 22-23 0.15979187422064456 1103 24-25 0.16585655982295577 1103 26-27 0.11525964181029735 1103 28-29 0.2531832338900415 1103 30-31 0.27804457267404104 1103 32-33 0.36878516107749704 1103 34-35 0.26761099444577496 1103 36-37 0.238638488146087 1103 38-39 0.4118497595214805 1103 40-41 0.4315318858271979 1103 42-43 0.43945991208676105 1103 44-45 0.49969752622902064 1103 46-47 0.6271925961783822 1103 48-49 0.9308712115028825 1103 50-51 0.5851086483838657 1103 52-53 0.6548533353958064 1103 54-55 0.6642909087611848 1103 56-57 0.2980218053647725 1103 58-59 0.5155717666919237 1103 60-61 0.5983159119230237 1103 62-63 0.6642000535981829 1103 64-65 0.4800347275805308 1103 66-67 0.2457161487783175 1103 68-69 0.2585929202891073 1103 70-71 0.36592687627681997 1103 72-73 0.2715914618852544 1103 74-75 0.1873980760772831 1103 76-77 0.40303391668020083 1103 78-79 0.265716038390746 1103 80-81 -0.04521965264533989 1103 82-83 0.3846849369875791 1103 84-85 0.8038431808450888 1103 86-87 0.7192932860844543 1103 88-89 0.7631237650861245 1103 90-91 0.4004457486659412 1103 92-93 0.3656298941636038 1103 94-95 0.35167458211628855 1103 96-97 0.14453183802885405 1103 98-99 0.3327404755389374 1103 100-101 0.46043634515229215 1104 1 0.3518283238237778 1104 2 0.3505858391685912 1104 3 0.11650460470170287 1104 4 0.09581601205800183 1104 5 0.0133435153614343 1104 6 0.14122989036584954 1104 7 0.08683461551158445 1104 8 0.05177382744427206 1104 9 -0.007696655233118577 1104 10-11 -0.07724299761025577 1104 12-13 0.13444138542627115 1104 14-15 0.32518384548267676 1104 16-17 0.17959705425185035 1104 18-19 0.078010938949582 1104 20-21 -0.03295155233769975 1104 22-23 3.515892503003215E-5 1104 24-25 0.2163851486299393 1104 26-27 0.33766882833732126 1104 28-29 0.2645446781395364 1104 30-31 0.11342846226354197 1104 32-33 0.2046965896451738 1104 34-35 0.14464618601985535 1104 36-37 0.3064541487049368 1104 38-39 0.12290800008712921 1104 40-41 0.4667812004858334 1104 42-43 0.32422512443432083 1104 44-45 0.3759141794010148 1104 46-47 0.6546744899120895 1104 48-49 0.4125743356621925 1104 50-51 0.3232829829564281 1104 52-53 0.2020040999992574 1104 54-55 0.5906818335160118 1104 56-57 0.6170080250493264 1104 58-59 0.5394314229517256 1104 60-61 0.19234973478278405 1104 62-63 0.08704879032605106 1104 64-65 0.18954053379000513 1104 66-67 0.17628604834906625 1104 68-69 0.13403893349857654 1104 70-71 0.1428848398188869 1104 72-73 0.21113838201559432 1104 74-75 0.32431117256048836 1104 76-77 0.6155041769983782 1104 78-79 0.7109697623303823 1104 80-81 0.7445098064697326 1104 82-83 0.5586868450514473 1104 84-85 0.6605832130485894 1104 86-87 0.6324150307949594 1104 88-89 0.7217286869460544 1104 90-91 0.5724893979233805 1104 92-93 0.31600120549164856 1104 94-95 0.1483913081201571 1104 96-97 0.2115033706083267 1104 98-99 0.5700240959785781 1104 100-101 0.5538343654376128 1105 1 0.24630919230378723 1105 2 0.3374650737725844 1105 3 0.12271817409343555 1105 4 0.04014470183383878 1105 5 -0.023717508907381557 1105 6 0.09161530608886892 1105 7 0.11271243176408063 1105 8 0.07096213557673536 1105 9 0.11998878171784355 1105 10-11 0.10161430324927068 1105 12-13 0.08889695614035276 1105 14-15 0.15820506937533452 1105 16-17 0.24416111736240254 1105 18-19 0.09154713665885339 1105 20-21 0.08223136632951622 1105 22-23 0.10896800153668806 1105 24-25 -0.05670700635842252 1105 26-27 -0.03754564831260865 1105 28-29 0.1125430988883025 1105 30-31 0.0855617088822811 1105 32-33 0.1443791304008144 1105 34-35 0.14458973590079438 1105 36-37 0.23027488205620728 1105 38-39 0.1814369708290613 1105 40-41 0.11904204465511015 1105 42-43 0.18705978575389537 1105 44-45 -0.20884685744231746 1105 46-47 0.12151224212919232 1105 48-49 0.2438790703060718 1105 50-51 0.13923091904543128 1105 52-53 0.3530720551258071 1105 54-55 0.3558229723346322 1105 56-57 0.20723492072545113 1105 58-59 0.09181814750624184 1105 60-61 0.08705167139369507 1105 62-63 0.06250110113026608 1105 64-65 0.1134589562694579 1105 66-67 0.20707130668779783 1105 68-69 -0.033544906759079396 1105 70-71 0.015832523065437698 1105 72-73 -0.031935154574224356 1105 74-75 0.00878407717938856 1105 76-77 -0.13865758267690254 1105 78-79 0.023481677699823678 1105 80-81 -0.19011757542106977 1105 82-83 -0.0018229502272966158 1105 84-85 0.21323832918710295 1105 86-87 0.03657016518680578 1105 88-89 0.22722395392469608 1105 90-91 0.027681186357799703 1105 92-93 0.10328634011335325 1105 94-95 0.12846745831668116 1105 96-97 0.09781114071369501 1105 98-99 0.3759242489009367 1105 100-101 0.4100667290007145 1106 1 0.353642980850136 1106 2 0.37061390401695604 1106 3 0.07473597901350004 1106 4 -0.0958204276403194 1106 5 -0.07615128273719307 1106 6 -0.05084462970238235 1106 7 -0.07002967099921165 1106 8 0.14890992464339803 1106 9 0.0069078707431415864 1106 10-11 -0.08704301132407721 1106 12-13 0.10172336165702944 1106 14-15 0.12276872398722816 1106 16-17 0.23877859544975166 1106 18-19 0.09754447512739972 1106 20-21 0.1829254499320072 1106 22-23 0.12616473097764924 1106 24-25 -0.1669699790574981 1106 26-27 0.09794311166900371 1106 28-29 0.19315956843936277 1106 30-31 -0.10187695767371707 1106 32-33 8.283572942744399E-4 1106 34-35 -0.001479936799185566 1106 36-37 0.12851646485835744 1106 38-39 0.03518578252083415 1106 40-41 0.27726035998102105 1106 42-43 0.1739524756792008 1106 44-45 0.28113124909008036 1106 46-47 0.30871289830549387 1106 48-49 0.03731953911503183 1106 50-51 0.2703263387143977 1106 52-53 0.36275899039604553 1106 54-55 0.1625562772578384 1106 56-57 0.17256648574918998 1106 58-59 0.015443319344502271 1106 60-61 -0.023603974840231245 1106 62-63 -0.18982215916206968 1106 64-65 0.18013115616888342 1106 66-67 0.23892773185210103 1106 68-69 0.28099792848333394 1106 70-71 0.01755177752974646 1106 72-73 0.24678860064020824 1106 74-75 0.2574595886488744 1106 76-77 0.3593743417512272 1106 78-79 0.4319404445375348 1106 80-81 0.5396173664555768 1106 82-83 0.5664159333802239 1106 84-85 0.5168691295785202 1106 86-87 0.5762415260342557 1106 88-89 0.56720094451099 1106 90-91 0.23975540932801565 1106 92-93 0.5153714086847536 1106 94-95 0.608098303345944 1106 96-97 0.40105659096656154 1106 98-99 0.45802876283317673 1106 100-101 0.5648604847242993 1107 1 0.6673627922013239 1107 2 0.6759521027912676 1107 3 0.4975969061562182 1107 4 0.279897870721598 1107 5 0.14992933196997882 1107 6 0.33624537431026624 1107 7 0.21617069967228275 1107 8 0.2532815356869875 1107 9 0.42113236224788864 1107 10-11 0.4347202555148115 1107 12-13 0.47533674178887964 1107 14-15 0.7069038316816929 1107 16-17 0.5445675374338279 1107 18-19 0.49831517895739097 1107 20-21 0.6135064646352575 1107 22-23 0.5494205794931375 1107 24-25 0.5183784561367943 1107 26-27 0.4669615209133866 1107 28-29 0.5422956167680226 1107 30-31 0.6587691547159338 1107 32-33 0.6496087770237366 1107 34-35 0.6700176144708223 1107 36-37 0.8635601091978629 1107 38-39 0.9606145141653286 1107 40-41 0.9621613142806993 1107 42-43 0.8566029762452132 1107 44-45 0.8039719861268182 1107 46-47 1.0834349943647297 1107 48-49 1.2575781899592258 1107 50-51 0.9409137040578059 1107 52-53 0.7543412023689626 1107 54-55 0.9625131059540273 1107 56-57 0.9270426539933077 1107 58-59 0.6291522200639186 1107 60-61 0.6118449940325696 1107 62-63 0.6902297623959228 1107 64-65 0.6813237135955994 1107 66-67 0.7716962376066441 1107 68-69 0.7191723441755045 1107 70-71 0.5827165226323778 1107 72-73 0.5870551390374779 1107 74-75 0.876179224197525 1107 76-77 0.8476013713371628 1107 78-79 1.059044230019289 1107 80-81 0.8875130100686697 1107 82-83 0.8612964184647396 1107 84-85 0.9592903546358542 1107 86-87 1.1569544651373427 1107 88-89 1.4450839702140463 1107 90-91 1.6684466373613098 1107 92-93 1.6970562639666404 1107 94-95 1.447536757414543 1107 96-97 1.3387838882435794 1107 98-99 1.8145708102694122 1107 100-101 1.769183813503684 1108 1 0.31120689452466266 1108 2 0.23826748454755986 1108 3 0.13934133255996883 1108 4 0.03203830122036777 1108 5 -0.03497908433967467 1108 6 -0.12209040429782192 1108 7 0.027558507206336458 1108 8 -0.01232746814068264 1108 9 0.07430111228864433 1108 10-11 0.009353301283837823 1108 12-13 0.20556425015171698 1108 14-15 0.054983801947152244 1108 16-17 -0.008305823416996816 1108 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2575.0 10 4155.0 11 6226.0 12 8522.0 13 11525.0 14 14890.0 15 19025.0 16 23389.0 17 27350.0 18 23679.0 19 17982.0 20 17484.0 21 18586.0 22 19695.0 23 22374.0 24 25903.0 25 30550.0 26 36375.0 27 43228.0 28 51562.0 29 59710.0 30 70600.0 31 82047.0 32 95562.0 33 112548.0 34 133789.0 35 157831.0 36 173363.0 37 142774.0 38 43298.0 39 974.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.046305158763815 22.32608136687519 16.40806210817236 20.219551366188632 2 16.992169663729484 23.586996589100988 37.388239230871264 22.032594516298257 3 20.86989037386996 26.48163910627409 31.009990795057725 21.63847972479822 4 14.356818759011256 19.370126922484697 36.498763170682444 29.774291147821604 5 12.84076828789534 39.17883015857252 33.79625587290204 14.184145680630103 6 29.971212865496295 38.52770370113635 16.634284750683687 14.866798682683664 7 26.817558619044036 34.89788532202732 20.535111439336728 17.749444619591912 8 26.601938382601613 34.71432484871591 19.233591699920616 19.45014506876186 9 26.356838197898913 16.968867624582494 20.603344329124536 36.07094984839406 10-11 24.935755436418127 27.690864924617824 26.56763900270709 20.80574063625696 12-13 26.63097434051679 25.135995760911406 27.626593615705957 20.60643628286585 14-15 23.061775051697218 26.23026049399379 27.155094987910932 23.552869466398057 16-17 20.984481341684425 29.683841412815777 27.70996645389026 21.62171079160954 18-19 20.896167025367205 27.32884852128508 31.324721409989714 20.450263043357996 20-21 22.054984160077527 25.986504239420444 31.537642227706968 20.42086937279506 22-23 21.624326471631157 26.150230486006876 31.407417676903677 20.818025365458293 24-25 21.725255161721844 26.49606118149225 31.495018072774993 20.283665584010908 26-27 21.431525555338933 26.415320706048202 31.664292177181906 20.48886156143096 28-29 20.828248317195698 26.814359307962356 31.869370795488706 20.488021579353248 30-31 21.12905021118786 26.51462385099742 31.570135230880286 20.78619070693444 32-33 21.019258783297612 26.692446359054482 31.497460634756326 20.79083422289158 34-35 21.801966451745347 26.40032953046907 31.158792161520587 20.638911856264993 36-37 21.926308864492107 26.120434129691013 31.21801866173784 20.73523834407904 38-39 21.39925869296145 26.46703409865742 31.45909247542029 20.674614732960837 40-41 21.572561361107287 26.627922749009603 31.144650809832974 20.654865080050136 42-43 21.42193176905907 26.83546590436043 30.71062259342101 21.03197973315949 44-45 21.75054405604194 26.74339550957972 30.26994511116073 21.236115323217614 46-47 22.09318950069269 27.32094765843158 29.465739879498116 21.12012296137761 48-49 22.28253279984802 27.38416108545708 29.09310274147237 21.240203373222528 50-51 22.012751954750957 27.667649346572297 29.018961967421596 21.300636731255153 52-53 22.392962205213028 27.904261782397448 28.585992330198067 21.116783682191457 54-55 21.77448679051974 27.60160952785028 28.856582642949252 21.767321038680727 56-57 22.22663990727083 27.862676575019513 28.47486107621029 21.43582244149937 58-59 22.560781544365955 27.39084516782015 28.458523446630917 21.589849841182982 60-61 22.034717373517545 27.539805666693884 29.063110832640398 21.36236612714817 62-63 22.187473338452353 27.46299377186247 29.07065176819384 21.27888112149134 64-65 21.946175914053185 27.73376006314286 28.78942970440783 21.530634318396125 66-67 21.853236404986124 27.834672580215674 28.508511891252553 21.80357912354565 68-69 21.98808721997252 27.738088136442755 28.59750393498993 21.67632070859479 70-71 22.0380641080416 27.840694362702134 28.45570018482835 21.665541344427922 72-73 22.101474149233198 27.560332008507498 28.53595525763452 21.802238584624778 74-75 21.931644052647332 28.334965204159374 28.059057949521538 21.674332793671763 76-77 22.063740635713376 27.944840898358308 28.239012553701244 21.75240591222707 78-79 22.02594675665542 27.915310586176727 28.287130775319753 21.771611881848102 80-81 21.95358355859384 28.312996003639725 28.05564906690487 21.677771370861567 82-83 22.14538615290933 28.10586979352584 28.066340768856197 21.682403284708634 84-85 21.864908795256174 28.170502595113327 27.947102548386958 22.01748606124354 86-87 22.133970673126278 28.046039378906997 27.831300485137255 21.988689462829473 88-89 22.072781566296747 28.087809636005495 27.726025241903518 22.113383555794233 90-91 21.995409977049885 28.05320693270133 27.955473110698886 21.9959099795499 92-93 22.09962040798643 28.166327288966503 27.945704011544397 21.788348291502672 94-95 21.795148249775117 27.936141429782204 27.927409385880686 22.341300934561996 96-97 21.893416380425716 27.74003078270596 27.90224324470251 22.464309592165808 98-99 21.833119195575264 27.93822833917421 27.48785580613284 22.740796659117684 100-101 21.848765543196986 27.988086715274363 27.57243493088135 22.590712810647297 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 837.0 1 625.5 2 504.5 3 591.5 4 493.5 5 458.0 6 771.0 7 1513.5 8 2349.0 9 2825.5 10 2571.5 11 2078.5 12 2064.0 13 2325.0 14 2561.5 15 2766.0 16 3016.0 17 3259.5 18 3547.5 19 3928.0 20 4380.5 21 4873.0 22 5513.5 23 6522.5 24 7640.0 25 8967.5 26 11271.5 27 13864.5 28 15983.5 29 18576.0 30 21339.5 31 24531.5 32 28928.0 33 32144.0 34 34829.0 35 37428.0 36 40282.0 37 43664.5 38 44625.5 39 43697.5 40 43510.0 41 45200.0 42 46650.0 43 47703.0 44 49219.0 45 51017.0 46 51507.0 47 54466.5 48 65417.0 49 65026.0 50 54229.0 51 48645.0 52 44077.0 53 42001.5 54 41409.0 55 38889.0 56 34891.0 57 31100.0 58 28283.0 59 25216.5 60 21461.0 61 17979.0 62 14912.0 63 11539.5 64 8754.0 65 7108.5 66 5471.5 67 4209.0 68 3043.0 69 2043.5 70 1565.5 71 1231.0 72 1009.0 73 888.5 74 746.0 75 639.0 76 483.5 77 317.5 78 241.0 79 168.5 80 108.0 81 81.5 82 60.0 83 38.0 84 19.0 85 12.0 86 5.5 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.976922969253954 2 0.008798105474621131 3 0.0027993971964703603 4 0.007731668447394328 5 0.0 6 0.0 7 0.0 8 0.0 9 0.003465920338487113 10-11 9.997847130251286E-5 12-13 3.332615710083762E-5 14-15 3.332615710083762E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.997847130251286E-4 24-25 0.0 26-27 3.332615710083762E-5 28-29 0.0 30-31 0.0 32-33 0.003099332610377898 34-35 4.332400423108891E-4 36-37 0.0022661786828569582 38-39 0.0 40-41 0.002966027981974548 42-43 0.009231345516932022 44-45 0.0011997416556301544 46-47 9.66458555924291E-4 48-49 0.008364865432310242 50-51 2.6660925680670097E-4 52-53 0.0015663293837393682 54-55 0.008764779317520293 56-57 0.0026660925680670093 58-59 7.665016133192652E-4 60-61 0.004365726580209729 62-63 0.0023328309970586333 64-65 0.015496663051889494 66-67 0.002632766410966172 68-69 9.997847130251286E-5 70-71 7.998277704201028E-4 72-73 0.0 74-75 0.0 76-77 5.665446707142396E-4 78-79 0.009031388574326995 80-81 0.013863681353948451 82-83 0.010631044115167201 84-85 0.006631905263066686 86-87 0.008598148532016105 88-89 0.026427642580964236 90-91 0.022028589843653665 92-93 0.0018662647976469067 94-95 0.006765209891470037 96-97 0.00866480084621778 98-99 0.008031603861301867 100-101 0.00993119481604961 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1500323.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.82579113334176 #Duplication Level Percentage of deduplicated Percentage of total 1 78.52118603754639 43.04986145236789 2 12.544186467992368 13.754898944636833 3 3.7652278021160286 6.192947791447946 4 1.7105311023835994 3.751248837854722 5 0.8986594868098181 2.463485866191559 6 0.5166879939170329 1.6996696821360364 7 0.3416822556986307 1.3113099989431538 8 0.24450446046484603 1.072412038449285 9 0.1929388953514168 0.9520224820231016 >10 1.0901448440819976 11.396773792938736 >50 0.11353241699390386 4.283888853975505 >100 0.05436551505607955 5.422036801556337 >500 0.004642373468040506 1.7274662356205828 >1k 0.0013438449512748832 1.3819194049600196 >5k 3.665031685295136E-4 1.5400578168983001 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9195 0.6128680290844039 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8518 0.5677444123698696 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5280 0.35192421898484527 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4980 0.3319285247243427 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2523 0.16816378873082663 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2335 0.15563315366091168 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2096 0.13970325056671132 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 6.665231420167524E-5 0.0 3 0.0 0.0 0.0 6.665231420167524E-5 0.0 4 0.0 0.0 0.0 6.665231420167524E-5 0.0 5 0.0 0.0 0.0 6.665231420167524E-5 0.0 6 0.0 0.0 0.0 6.665231420167524E-5 0.0 7 0.0 0.0 0.0 6.665231420167524E-5 0.0 8 0.0 0.0 0.0 6.665231420167524E-5 0.0 9 0.0 0.0 0.0 6.665231420167524E-5 0.0 10-11 0.0 0.0 0.0 6.665231420167524E-5 0.0 12-13 0.0 0.0 0.0 6.665231420167524E-5 0.0 14-15 0.0 0.0 0.0 6.665231420167524E-5 0.0 16-17 0.0 0.0 0.0 6.665231420167524E-5 3.332615710083762E-5 18-19 0.0 0.0 0.0 6.665231420167524E-5 6.665231420167524E-5 20-21 0.0 0.0 0.0 1.3330462840335048E-4 6.665231420167524E-5 22-23 0.0 0.0 0.0 1.6663078550418808E-4 6.665231420167524E-5 24-25 0.0 0.0 0.0 2.6660925680670097E-4 6.665231420167524E-5 26-27 0.0 0.0 0.0 5.332185136134019E-4 6.665231420167524E-5 28-29 0.0 0.0 0.0 0.0010997631843276414 6.665231420167524E-5 30-31 0.0 0.0 0.0 0.0023328309970586333 6.665231420167524E-5 32-33 0.0 0.0 0.0 0.00463233583701643 6.665231420167524E-5 34-35 0.0 0.0 0.0 0.008531496217814431 6.665231420167524E-5 36-37 0.0 0.0 0.0 0.014896792224074416 6.665231420167524E-5 38-39 0.0 0.0 0.0 0.026294337952560882 6.665231420167524E-5 40-41 0.0 0.0 0.0 0.04002471467810598 9.997847130251286E-5 42-43 0.0 0.0 0.0 0.053921722189155266 1.3330462840335048E-4 44-45 0.0 0.0 0.0 0.06835194821381796 1.3330462840335048E-4 46-47 0.0 0.0 0.0 0.08698127003318619 1.3330462840335048E-4 48-49 0.0 0.0 0.0 0.11014294921826834 1.3330462840335048E-4 50-51 0.0 0.0 0.0 0.13413778233087142 1.3330462840335048E-4 52-53 0.0 0.0 0.0 0.16083203416864233 1.3330462840335048E-4 54-55 0.0 0.0 0.0 0.19212529568632886 1.3330462840335048E-4 56-57 0.0 0.0 0.0 0.23158346569372062 1.3330462840335048E-4 58-59 0.0 0.0 0.0 0.2794398272905234 1.3330462840335048E-4 60-61 0.0 0.0 0.0 0.3340613987787963 1.3330462840335048E-4 62-63 0.0 0.0 0.0 0.3894494718803884 1.3330462840335048E-4 64-65 0.0 0.0 0.0 0.44820348684916517 1.3330462840335048E-4 66-67 0.0 0.0 0.0 0.5194881368878568 1.3330462840335048E-4 68-69 0.0 0.0 0.0 0.6044365113378919 1.3330462840335048E-4 70-71 0.0 0.0 0.0 0.7019488470149428 1.3330462840335048E-4 72-73 0.0 0.0 0.0 0.8056598479127495 1.3330462840335048E-4 74-75 0.0 0.0 0.0 0.9132033568771525 1.3330462840335048E-4 76-77 0.0 0.0 0.0 1.0327776085549578 1.3330462840335048E-4 78-79 0.0 0.0 0.0 1.1717476836654508 1.3330462840335048E-4 80-81 0.0 0.0 0.0 1.3292137759669085 1.3330462840335048E-4 82-83 0.0 0.0 0.0 1.4901791147639543 1.3330462840335048E-4 84-85 0.0 0.0 0.0 1.6584762081231843 1.3330462840335048E-4 86-87 0.0 0.0 0.0 1.8415034629209843 1.3330462840335048E-4 88-89 0.0 0.0 0.0 2.046992547604749 1.3330462840335048E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGGT 640 0.0 23.74736 4 TAAGGTG 765 0.0 22.96977 5 ATACGTC 170 3.1549152E-6 22.348965 5 GGTAAGG 735 0.0 21.971079 3 TAGCGCA 130 2.9443012E-4 21.920637 4 AGTACTT 9925 0.0 21.484484 12-13 GAGTACT 9865 0.0 21.470736 12-13 AGGTAAG 745 0.0 21.040735 2 GTACTTT 10330 0.0 20.54987 14-15 GTACATG 9220 0.0 20.393497 1 GCACCGT 335 3.45608E-11 19.847878 6 TACATGG 9555 0.0 19.33847 2 TACTTTT 11340 0.0 18.782408 14-15 ACATGGG 9530 0.0 18.68952 3 GGTATCA 16195 0.0 18.037357 1 GTATCAA 19720 0.0 17.975216 1 ACTTTTT 12045 0.0 17.880796 16-17 TGCACCG 335 1.4611942E-8 17.0119 5 TATAAGC 735 0.0 16.801413 3 AAGGTAA 925 0.0 16.593761 1 >>END_MODULE