FastQCFastQC Report
Thu 26 May 2016
SRR936421_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936421_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1522459
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97570.6408711170547121No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73560.48316572071891595No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44410.29169915248949235No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT29990.1969839581886934No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29800.1957359771264776No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA26080.17130182159256832No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA20810.1366867679195302No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC19090.12538925514578717No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18780.1233530755179614No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT18680.12269624337995308No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT16850.11067621525440094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG94400.025.728331
GTATCAA154900.025.5290471
GTATAGG5500.025.0609761
TACATGG97300.024.3272742
ACATGGG95650.023.5046163
GAGTACT79650.023.47818612-13
TCAACGC176450.022.250274
ATCAACG176750.022.2125073
CAACGCA178050.022.1015365
TATCAAC178550.022.01522
GTACTTT85350.021.71534314-15
AACGCAG181500.021.7068966
GGTATCA112950.021.4608361
AGTACTT82650.021.21724912-13
CATGGGG34450.020.6956464
AATTCGC2350.020.21632278-79
CATGGGA53500.019.9896744
ACGCAGA197550.019.896517
CGCAGAG198400.019.786018
CAATTCG2300.019.62301678-79
Produced by FastQC (version 0.11.5)