##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936421_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1522459 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.05568294449966 33.0 31.0 34.0 31.0 34.0 2 32.147465383304244 33.0 31.0 34.0 31.0 34.0 3 32.204444257612195 34.0 31.0 34.0 30.0 34.0 4 35.81355951129061 37.0 35.0 37.0 35.0 37.0 5 35.80407157105709 37.0 35.0 37.0 35.0 37.0 6 35.94743109666664 37.0 35.0 37.0 35.0 37.0 7 35.85521383498669 37.0 35.0 37.0 35.0 37.0 8 35.82603735141636 37.0 35.0 37.0 35.0 37.0 9 37.43996455733783 39.0 37.0 39.0 35.0 39.0 10-11 37.3786276017942 39.0 37.0 39.0 35.0 39.0 12-13 37.29906421125298 39.0 37.0 39.0 34.5 39.0 14-15 38.48620225569293 40.0 38.0 41.0 34.0 41.0 16-17 38.38715886601872 40.0 38.0 41.0 34.0 41.0 18-19 38.37051473964159 40.0 38.0 41.0 34.0 41.0 20-21 38.30608935938505 40.0 38.0 41.0 34.0 41.0 22-23 38.17954112393174 40.0 38.0 41.0 34.0 41.0 24-25 38.323364044614664 40.0 38.0 41.0 34.0 41.0 26-27 38.30063699580744 40.0 38.0 41.0 34.0 41.0 28-29 38.20850479388936 40.0 38.0 41.0 34.0 41.0 30-31 38.01351530648773 40.0 37.5 41.0 34.0 41.0 32-33 37.813030761419526 40.0 37.0 41.0 33.0 41.0 34-35 37.609908050069 40.0 37.0 41.0 33.0 41.0 36-37 37.42740756893946 40.0 37.0 41.0 33.0 41.0 38-39 37.235874660664095 39.0 36.0 41.0 32.5 41.0 40-41 37.04208487716254 39.0 36.0 41.0 32.0 41.0 42-43 36.789626518678006 39.0 36.0 41.0 31.0 41.0 44-45 36.497442952486736 39.0 35.5 40.5 30.5 41.0 46-47 36.129374255727086 39.0 35.0 40.5 30.0 41.0 48-49 35.951536625945266 39.0 35.0 41.0 29.5 41.0 50-51 35.04306716962493 38.0 34.0 40.0 28.0 40.5 52-53 35.17169362196289 38.5 35.0 39.5 28.0 40.5 54-55 35.58354740587431 39.0 35.0 40.0 28.0 41.0 56-57 35.49915629911873 39.0 35.0 41.0 28.0 41.0 58-59 35.267263683291304 38.5 35.0 40.0 27.5 41.0 60-61 34.97115193249868 38.0 35.0 40.0 27.0 41.0 62-63 34.65756680475468 38.0 34.0 40.0 26.0 41.0 64-65 34.32455291078446 37.0 34.0 40.0 26.0 41.0 66-67 33.95575151777486 37.0 34.0 39.5 26.0 41.0 68-69 33.5681194042007 36.0 34.0 39.0 25.0 41.0 70-71 33.135168172016456 36.0 33.0 39.0 23.5 40.5 72-73 32.746278224898006 35.0 33.0 38.0 22.5 40.0 74-75 32.32853101462831 35.0 32.5 37.0 21.5 39.5 76-77 31.90209292992455 35.0 32.0 37.0 20.5 39.0 78-79 31.475225277002533 35.0 32.0 36.0 19.5 39.0 80-81 31.027584979299935 35.0 31.0 36.0 17.5 37.5 82-83 30.559944142994986 34.5 31.0 35.5 12.5 37.0 84-85 30.258101531798232 34.0 31.0 35.0 9.5 37.0 86-87 29.96987110982956 34.0 30.5 35.0 7.0 36.0 88-89 29.597060413449558 34.0 30.0 35.0 2.0 36.0 90-91 29.228224208336645 34.0 29.5 35.0 2.0 35.5 92-93 28.904296601747568 34.0 29.0 35.0 2.0 35.0 94-95 28.50759495001179 34.0 29.0 35.0 2.0 35.0 96-97 27.99814346396192 34.0 29.0 35.0 2.0 35.0 98-99 27.380841782931427 34.0 27.5 35.0 2.0 35.0 100-101 24.8234622410193 31.5 21.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3024662923050201 1101 2 0.29667444476989857 1101 3 0.3019291051576616 1101 4 0.4236162902701608 1101 5 0.3780243643893826 1101 6 0.2047249963773794 1101 7 0.28225912823162247 1101 8 0.27557025415183745 1101 9 0.4791417852348516 1101 10-11 0.35865484702868855 1101 12-13 0.41249547716147816 1101 14-15 0.5622986334363844 1101 16-17 0.6059725696455232 1101 18-19 0.5487077492864074 1101 20-21 0.6117437943315949 1101 22-23 0.6164618336917371 1101 24-25 0.5879480933774985 1101 26-27 0.4585384388972358 1101 28-29 0.47048407296434647 1101 30-31 0.5820320835212911 1101 32-33 0.49655303073244994 1101 34-35 0.48759841893537725 1101 36-37 0.49776241752584127 1101 38-39 0.4765013909229552 1101 40-41 0.3963649489015424 1101 42-43 0.40411791032092736 1101 44-45 0.362816868895095 1101 46-47 0.436821283096684 1101 48-49 0.6829160686628981 1101 50-51 0.5985256829544099 1101 52-53 0.6692043812128006 1101 54-55 0.6430502701655243 1101 56-57 0.5848063245585564 1101 58-59 0.5058782658928891 1101 60-61 0.41546154748461817 1101 62-63 0.4355454497885205 1101 64-65 0.5227082600425916 1101 66-67 0.371296889173955 1101 68-69 0.5113946595899748 1101 70-71 0.5854044015183106 1101 72-73 0.4534694042018472 1101 74-75 0.5394100463865499 1101 76-77 0.5036482508385802 1101 78-79 0.4210797178160952 1101 80-81 0.5448320187580649 1101 82-83 0.7691821269601142 1101 84-85 0.6206184911087576 1101 86-87 0.5174945352622942 1101 88-89 0.6613485841029245 1101 90-91 0.581424052867888 1101 92-93 0.4168993257255842 1101 94-95 0.5561513072032724 1101 96-97 0.4390522153843577 1101 98-99 0.341250427325857 1101 100-101 0.8253911870860797 1102 1 0.2181267922697856 1102 2 0.3411843054906427 1102 3 0.2517541253175324 1102 4 0.24320839543030814 1102 5 0.3058449846087541 1102 6 0.1898525711646215 1102 7 0.23027845039025863 1102 8 0.2832173820554402 1102 9 0.3659386755260172 1102 10-11 0.3804212430703089 1102 12-13 0.3052964855464211 1102 14-15 0.29223263432890434 1102 16-17 0.19684887807978413 1102 18-19 0.31522294943673046 1102 20-21 0.46795655729066965 1102 22-23 0.2330005993596771 1102 24-25 0.12810451830840464 1102 26-27 0.14294934010212756 1102 28-29 0.14973013298205018 1102 30-31 0.18838741394743153 1102 32-33 0.028667742348453373 1102 34-35 -0.1157737205161169 1102 36-37 0.08335785787219407 1102 38-39 0.048791943577406016 1102 40-41 -0.035508000859273636 1102 42-43 0.1864777502946211 1102 44-45 0.02006620146639193 1102 46-47 0.2556383097703474 1102 48-49 0.46470454269776695 1102 50-51 0.5076869540831339 1102 52-53 0.5164816635840666 1102 54-55 0.44433611754552516 1102 56-57 0.4782300576183971 1102 58-59 0.5317503857716943 1102 60-61 0.5250490334901485 1102 62-63 0.586009635682089 1102 64-65 0.5565809122985357 1102 66-67 0.6940295497287252 1102 68-69 0.4952672335551753 1102 70-71 0.4149456959776714 1102 72-73 0.5145974174173276 1102 74-75 0.39606984956358815 1102 76-77 0.5312228815019324 1102 78-79 0.4497433728983431 1102 80-81 0.46724353063086 1102 82-83 0.5127524986063356 1102 84-85 0.4907206605456125 1102 86-87 0.657666931507471 1102 88-89 0.6542366518087697 1102 90-91 0.8983689909790442 1102 92-93 0.8146367228711036 1102 94-95 0.8609401961791505 1102 96-97 0.7541912989127617 1102 98-99 0.7941118761745933 1102 100-101 1.1890238781742433 1103 1 0.19129022753118363 1103 2 0.29804464086684845 1103 3 0.19170011584161983 1103 4 0.2653745958475042 1103 5 0.3153629419509727 1103 6 0.17669883820428822 1103 7 0.21057081306226877 1103 8 0.23042813220502012 1103 9 0.38594611395683387 1103 10-11 0.3483987984138537 1103 12-13 0.36034378426410285 1103 14-15 0.2508408392414978 1103 16-17 0.406367482242068 1103 18-19 0.35849715263525184 1103 20-21 0.4056427201923043 1103 22-23 0.4084946890412837 1103 24-25 0.6005611409374083 1103 26-27 0.3767185223598588 1103 28-29 0.10567877546841942 1103 30-31 0.1383131913443023 1103 32-33 0.2602993459771099 1103 34-35 0.20331846751447813 1103 36-37 0.25918651523543446 1103 38-39 0.27716147188386486 1103 40-41 0.21070082500058618 1103 42-43 0.2931883468244365 1103 44-45 0.18032072516426467 1103 46-47 0.3079180271099773 1103 48-49 0.20941695032377794 1103 50-51 0.2752596000623342 1103 52-53 0.27880730514872454 1103 54-55 0.24446855395503064 1103 56-57 0.24092826754385044 1103 58-59 0.0339377695462133 1103 60-61 0.09401622354206296 1103 62-63 0.2748396998789815 1103 64-65 0.34872398889820744 1103 66-67 0.27127959419760117 1103 68-69 0.3317593666060574 1103 70-71 0.48640039431696636 1103 72-73 0.47815670656206777 1103 74-75 0.360402573808031 1103 76-77 0.3548217883760145 1103 78-79 0.31309907542579296 1103 80-81 0.14578123215573768 1103 82-83 0.23383559881493454 1103 84-85 0.3413558378551649 1103 86-87 0.4926175306245639 1103 88-89 0.5357853007633473 1103 90-91 0.5712682002415086 1103 92-93 0.38193188303992187 1103 94-95 0.30654634743802234 1103 96-97 0.24287437324856143 1103 98-99 0.36781847859046835 1103 100-101 0.9687976528544695 1104 1 0.18659609098040875 1104 2 0.28452332047225326 1104 3 0.22365361723137767 1104 4 0.16671296657460033 1104 5 0.33728597566219776 1104 6 0.19071476808674248 1104 7 0.32233028659567253 1104 8 0.3060977330671264 1104 9 0.38313335489348077 1104 10-11 0.43474405312112196 1104 12-13 0.2504603698365244 1104 14-15 0.33236149084961397 1104 16-17 0.30685059072974497 1104 18-19 0.4266425731430701 1104 20-21 0.5459261699871192 1104 22-23 0.3327727353604004 1104 24-25 0.41327117546035197 1104 26-27 0.15887894203334696 1104 28-29 0.3598791335284588 1104 30-31 0.3966482586916342 1104 32-33 0.18474691570940394 1104 34-35 0.18362815687444822 1104 36-37 0.19712299647451204 1104 38-39 0.23327451940293997 1104 40-41 0.31545964636745083 1104 42-43 0.17891012100994175 1104 44-45 0.4574044172113574 1104 46-47 0.4279623757644515 1104 48-49 0.40933391709120315 1104 50-51 0.3612840898799661 1104 52-53 0.4226794991207399 1104 54-55 0.6212478018959828 1104 56-57 0.5086519403915801 1104 58-59 0.5792824571810442 1104 60-61 0.581966100454764 1104 62-63 0.7877294624597866 1104 64-65 0.5981986325167767 1104 66-67 0.7387267776824302 1104 68-69 0.5855819533737971 1104 70-71 0.6672268641680574 1104 72-73 0.5678334287371243 1104 74-75 0.413592705285069 1104 76-77 0.5102116024786341 1104 78-79 0.5221995576035603 1104 80-81 0.44919872444574693 1104 82-83 0.49588289651201123 1104 84-85 0.5255368768940798 1104 86-87 0.6931853343390522 1104 88-89 0.6663450870175929 1104 90-91 0.7529537549187921 1104 92-93 0.5645262161713802 1104 94-95 0.4594523111387403 1104 96-97 0.6788412251057068 1104 98-99 0.9839760874992898 1104 100-101 1.4541370354036651 1105 1 0.2167648111448841 1105 2 0.3071888510604026 1105 3 0.3104274610729192 1105 4 0.31939788992577434 1105 5 0.3431406240724115 1105 6 0.2612984510027374 1105 7 0.35396796600825553 1105 8 0.3908402518476777 1105 9 0.48535332780682694 1105 10-11 0.450133216168652 1105 12-13 0.3001498529011215 1105 14-15 0.24360078323433498 1105 16-17 0.348582024408266 1105 18-19 0.324565362644222 1105 20-21 0.3908542312769967 1105 22-23 0.24781138368168598 1105 24-25 0.3682582903708962 1105 26-27 0.28850929853297913 1105 28-29 0.2619595744177019 1105 30-31 0.2077332912508396 1105 32-33 0.3035535539185261 1105 34-35 0.17900783021597988 1105 36-37 0.2793313955590264 1105 38-39 0.13171226092238442 1105 40-41 -0.007300121308425389 1105 42-43 0.30830983458199057 1105 44-45 0.11500439692589737 1105 46-47 0.21198698369752833 1105 48-49 0.27347394374712763 1105 50-51 0.4401195098255073 1105 52-53 0.2928063423102927 1105 54-55 0.2498607590960873 1105 56-57 0.42766726876771344 1105 58-59 0.6221509535454643 1105 60-61 0.50370259855179 1105 62-63 0.3871990879324869 1105 64-65 0.5528113000238122 1105 66-67 0.5732630551403162 1105 68-69 0.6546496106008917 1105 70-71 0.7047186709601405 1105 72-73 0.6568360682787073 1105 74-75 0.564239877529829 1105 76-77 0.4230827630467502 1105 78-79 0.7210670799879146 1105 80-81 0.4976168925781863 1105 82-83 0.639821451013038 1105 84-85 0.643037464790396 1105 86-87 0.8395899271900262 1105 88-89 0.7099807142638888 1105 90-91 0.751744963671829 1105 92-93 0.6064082277250584 1105 94-95 0.6195895367335744 1105 96-97 0.9057860503850073 1105 98-99 0.9326180904247003 1105 100-101 1.2107925948534408 1106 1 0.08541754585473882 1106 2 0.17282551224779752 1106 3 0.1959692650988174 1106 4 0.23549297906504307 1106 5 0.30267065160164464 1106 6 0.1646093900233936 1106 7 0.16347108049109949 1106 8 0.25408500470980044 1106 9 0.4141187529695287 1106 10-11 0.29086760705482106 1106 12-13 0.42357145256909945 1106 14-15 0.47546909235196466 1106 16-17 0.5372914124400197 1106 18-19 0.5622345659636849 1106 20-21 0.3752881602011868 1106 22-23 0.4926780598932723 1106 24-25 0.362972821784858 1106 26-27 0.19326719891665078 1106 28-29 0.3544541872776037 1106 30-31 0.30933375665625107 1106 32-33 0.23614316892604137 1106 34-35 0.30033963636230965 1106 36-37 0.25793851401449075 1106 38-39 0.2616339503005207 1106 40-41 0.27132149575788134 1106 42-43 0.18153923703986408 1106 44-45 0.30644550447478025 1106 46-47 0.3337405181870139 1106 48-49 0.3478993003676507 1106 50-51 0.299170720731361 1106 52-53 0.46920622849619775 1106 54-55 0.4661283817045003 1106 56-57 0.3733034587502644 1106 58-59 0.29109764759034107 1106 60-61 0.43606984111535496 1106 62-63 0.481130694360445 1106 64-65 0.4742150555264928 1106 66-67 0.4610871217469281 1106 68-69 0.563807295680185 1106 70-71 0.4099450577069632 1106 72-73 0.4549880390930028 1106 74-75 0.24510606078865038 1106 76-77 0.2895514632644165 1106 78-79 0.4269817882849196 1106 80-81 0.5352746782415991 1106 82-83 0.5852089898194741 1106 84-85 0.5424239923187315 1106 86-87 0.6460963600424279 1106 88-89 0.7028941557447759 1106 90-91 0.8028522044593238 1106 92-93 0.9697109750305835 1106 94-95 0.8794151546915536 1106 96-97 0.8358686743435975 1106 98-99 1.1777908685578957 1106 100-101 1.6107012536050753 1107 1 0.16589921170605493 1107 2 0.22307291775756966 1107 3 0.18110178109148478 1107 4 0.12449323674021429 1107 5 0.14361897092467046 1107 6 0.22797544934843472 1107 7 0.24028106185632936 1107 8 0.22880034833271168 1107 9 0.31926516101496816 1107 10-11 0.41021023661128453 1107 12-13 0.39413803919463675 1107 14-15 0.3549193106289792 1107 16-17 0.38097220891771855 1107 18-19 0.44847265782301804 1107 20-21 0.4894672033673757 1107 22-23 0.44118092979719137 1107 24-25 0.5110979548236614 1107 26-27 0.3070683184965972 1107 28-29 0.34646980463370625 1107 30-31 0.4730448766469877 1107 32-33 0.2839745896125052 1107 34-35 0.46423150241602684 1107 36-37 0.43882069670991797 1107 38-39 0.3563208002775511 1107 40-41 0.35424808820912546 1107 42-43 0.5164906335272761 1107 44-45 0.6495592288519703 1107 46-47 0.6611836709361043 1107 48-49 0.6843991752644882 1107 50-51 0.6303882545932353 1107 52-53 0.5395177540452494 1107 54-55 0.5004645320374195 1107 56-57 0.36307450128906993 1107 58-59 0.6751604381751193 1107 60-61 0.6027096821783218 1107 62-63 0.6602668476809086 1107 64-65 0.723095475574361 1107 66-67 0.6272696827393958 1107 68-69 0.6903749130064156 1107 70-71 0.7466858028736922 1107 72-73 0.615820105834608 1107 74-75 0.5492184071638349 1107 76-77 0.4288398316577542 1107 78-79 0.5899202562006245 1107 80-81 0.7718936812254036 1107 82-83 0.9779808277903399 1107 84-85 0.7767287735002242 1107 86-87 0.5740429129834723 1107 88-89 0.5977184324804377 1107 90-91 0.4864762003663934 1107 92-93 0.7155211393825311 1107 94-95 0.6731308680223194 1107 96-97 0.6845278298412261 1107 98-99 1.0604398066747756 1107 100-101 1.4891502731323705 1108 1 0.04424450565143445 1108 2 0.2268242913061158 1108 3 0.15728881830437302 1108 4 0.1749073561409844 1108 5 0.2954011726082797 1108 6 0.19962389114388657 1108 7 0.06409617922939503 1108 8 0.28330139809487065 1108 9 0.2176860091078865 1108 10-11 0.12842131581146532 1108 12-13 0.329577666499155 1108 14-15 0.29952729967410363 1108 16-17 0.47404234228658737 1108 18-19 0.2159156271297462 1108 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2270.0 3 697.0 4 635.0 5 681.0 6 771.0 7 873.0 8 1096.0 9 1324.0 10 1696.0 11 2560.0 12 3681.0 13 5307.0 14 6177.0 15 8732.0 16 12062.0 17 17777.0 18 14499.0 19 7506.0 20 6808.0 21 6790.0 22 7221.0 23 7850.0 24 8774.0 25 10233.0 26 12168.0 27 14703.0 28 18306.0 29 22489.0 30 29292.0 31 37954.0 32 51247.0 33 71892.0 34 103799.0 35 157114.0 36 235070.0 37 324286.0 38 265517.0 39 42587.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.15336873899049 19.103154511330313 14.737456955713583 22.00601979396562 2 15.230586391142475 20.548162095364358 40.57166845909807 23.649583054395094 3 19.435902253539815 25.788686380021325 29.956910568571583 24.818500797867273 4 12.330073290185897 16.911613468361285 38.07199431710278 32.68631892435005 5 13.146833569694587 38.19608827599985 33.94638301563664 14.71069513866892 6 30.40065816968897 36.56883051341967 17.995710331739602 15.034800985151756 7 27.673761475124365 33.99225901417429 20.763190232426716 17.57078927827464 8 26.602237879684893 33.37100586311599 19.963505831904733 20.063250425294388 9 26.809315357250334 16.897062437494824 21.02644502764355 35.26717717761129 10-11 24.647028905319768 26.758152866744293 27.084116399307135 21.510701828628804 12-13 26.37166775085914 24.56086919382076 27.673684348860284 21.393778706459816 14-15 23.20517676788417 25.4215716840232 27.15243928280332 24.220812265289315 16-17 21.410388671682494 29.09462167756349 27.503630449781514 21.9913592009725 18-19 21.178630968378705 27.165852603938383 30.719494332664603 20.936022095018302 20-21 22.179254024053506 25.973542260814042 30.580080528694957 21.267123186437495 22-23 21.700569889471005 26.087864803166415 30.520323868022135 21.691241439340438 24-25 21.679796163817763 26.350023876789198 30.726077723382634 21.244102236010402 26-27 21.400653862247502 26.364298622054616 30.824014887617018 21.411032628080868 28-29 20.940787377783032 26.722007100653904 31.008476775081366 21.328728746481694 30-31 21.108714972900575 26.47427640165874 30.852947107675792 21.564061517764888 32-33 20.837152630101038 26.68290175690388 30.690659130431925 21.789286482563153 34-35 21.73227472355676 26.535299473059837 30.281455148340687 21.450970655042717 36-37 21.71313393266516 26.130742885069385 30.582607816012224 21.57351536625323 38-39 21.391851617806545 26.471830518695246 30.560834347671577 21.575483515826633 40-41 21.42778322224003 26.618417491874908 30.718863879393922 21.234935406491143 42-43 21.266407039747353 26.787113589295085 30.093481455110577 21.852997915846988 44-45 21.340270887789554 26.958231810348988 29.81302693015186 21.888470371709595 46-47 21.716902349294596 27.081126100770003 29.200036519914846 22.001935030020555 48-49 21.82750769236832 27.268834027772986 28.861857906053434 22.041800373805255 50-51 21.6800140562216 27.544644103365773 28.956506175049224 21.8188356653634 52-53 21.89925408427613 27.644302769793434 28.628161405758185 21.82828174017225 54-55 21.394029762510797 27.355826548474926 29.003405840178402 22.246737848835874 56-57 21.78506699494639 27.774409290281216 28.453818255392083 21.98670545938031 58-59 21.906596779400882 27.249169297730198 28.587390667034956 22.256843255833967 60-61 21.708669765388727 27.286566540633284 29.170465622840506 21.83429807113748 62-63 21.699299266160622 27.42501333417411 29.098419342040614 21.777268057624653 64-65 21.430100909121364 27.636671989196426 28.866163226727288 22.067063874954925 66-67 21.39060932761335 27.79904925370291 28.486918206522276 22.32342321216146 68-69 21.492853518059583 27.6015312834106 28.80793429473757 22.09768090379224 70-71 21.539397836847556 27.677427470413562 28.53294043497553 22.25023425776335 72-73 21.535016114141918 27.506428086064265 28.67601296750799 22.282542832285824 74-75 21.40213512612549 28.155518369934168 28.341639885903273 22.10070661803707 76-77 21.579857490298732 27.706428044753107 28.393719588597254 22.31999487635091 78-79 21.542223883371552 27.81975902094953 28.4869411110155 22.151075984663418 80-81 21.58253768732812 28.194558809377025 28.14240073625653 22.080502767038332 82-83 21.676071339542684 28.083696497888344 28.16386694136785 22.076365221201126 84-85 21.519842577152072 28.03876054798444 28.047825959857963 22.393570915005522 86-87 21.66300855449777 28.00684175903359 28.203207337287356 22.126942349181284 88-89 21.625676401772473 27.969237923953983 27.87586524907481 22.529220425198734 90-91 21.428024835500118 28.000564891036788 28.17466299487493 22.396747278588165 92-93 21.694383587291057 27.980239581930515 28.220675519877027 22.104701310901405 94-95 21.40021742914667 27.772040976526192 28.213468126266555 22.614273468060578 96-97 21.515999785857183 27.58905073312714 28.238934777417917 22.656014703597762 98-99 21.37244081814987 27.812628308090513 27.836179514720328 22.978751359039297 100-101 21.436291731522687 27.682181080855557 28.048287702510116 22.83323948511164 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 597.0 1 457.5 2 492.5 3 649.0 4 512.0 5 456.5 6 844.5 7 1689.0 8 2752.0 9 3307.5 10 2808.5 11 2203.5 12 2170.5 13 2304.5 14 2473.5 15 2644.5 16 2789.5 17 2936.0 18 3063.0 19 3258.0 20 3651.5 21 4149.5 22 4846.5 23 5777.5 24 6740.0 25 8152.5 26 10364.5 27 12829.5 28 14854.5 29 17193.5 30 20471.5 31 24085.5 32 28151.0 33 31340.5 34 34626.5 35 37440.0 36 40353.0 37 43691.5 38 44239.5 39 43628.5 40 43908.5 41 45465.5 42 46584.5 43 48379.0 44 50347.0 45 52330.0 46 52639.5 47 54983.5 48 68703.5 49 68118.0 50 54618.0 51 49546.0 52 45393.5 53 44036.5 54 43867.0 55 41064.5 56 37120.0 57 32979.0 58 30076.5 59 27083.0 60 23040.5 61 19077.5 62 15791.0 63 12389.5 64 9253.5 65 7427.0 66 5659.5 67 4346.0 68 3128.5 69 2123.5 70 1635.0 71 1306.0 72 1144.0 73 1049.5 74 836.0 75 674.0 76 522.0 77 332.5 78 232.5 79 174.5 80 121.0 81 83.0 82 56.5 83 31.0 84 19.5 85 12.5 86 10.0 87 8.0 88 5.0 89 5.0 90 6.0 91 3.5 92 5.5 93 4.5 94 0.0 95 0.5 96 2.0 97 2.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03152794262439908 2 0.02088726198866439 3 0.018128567009029473 4 0.04597824966058199 5 0.051035857123246015 6 0.04354797074995123 7 0.045255734308772846 8 0.03757079829407557 9 0.03842468007348638 10-11 0.04354797074995123 12-13 0.038687412928689706 14-15 0.03320286457632028 16-17 0.04000107720470633 18-19 0.043876386818955386 20-21 0.00949122439422014 22-23 0.015369872029394552 24-25 0.003940992828049885 26-27 0.00788198565609977 28-29 0.004203725683253211 30-31 0.006699687807684804 32-33 0.002397437303730347 34-35 0.005583073173070671 36-37 0.0063712717386806466 38-39 0.00788198565609977 40-41 9.852482070124712E-4 42-43 0.0011822978484149656 44-45 0.0013465058829170442 46-47 1.3136642760166285E-4 48-49 5.583073173070671E-4 50-51 9.852482070124712E-5 52-53 0.0022989124830291 54-55 0.0048605578212615255 56-57 0.011822978484149656 58-59 0.0067325294145852204 60-61 0.007290836731892287 62-63 0.003612576759045728 64-65 0.0 66-67 0.004991924248863188 68-69 0.013563583649871688 70-71 0.006404113345581063 72-73 0.0025288037313320097 74-75 0.004367933717755289 76-77 0.006995262269788547 78-79 0.005648756386871502 80-81 0.010739205456435938 82-83 0.0050904490695644344 84-85 0.012446969015257554 86-87 0.0028572198003361665 88-89 0.004269408897054042 90-91 0.0027258533727345037 92-93 0.014713039891386238 94-95 0.008144718511303096 96-97 0.007257995124991872 98-99 0.016026704167402865 100-101 0.032710240472814046 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1522459.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.86259533850777 #Duplication Level Percentage of deduplicated Percentage of total 1 77.08940824926565 40.75146193165952 2 12.974364299378074 13.717171394648098 3 4.2459875730350625 6.733617686570555 4 1.8959112794729769 4.008911630579699 5 1.0097158523969005 2.6688100256066893 6 0.579061418315907 1.8366413659545695 7 0.3830450228433131 1.4174127827296865 8 0.26455405627997264 1.1188011217831204 9 0.2055188888095935 0.977783566820518 >10 1.1615406969228528 11.678796229530377 >50 0.12112401472466162 4.420519412458549 >100 0.06377787106359081 6.261841223933354 >500 0.004368275254177578 1.623751980692176 >1k 0.0013728865084558103 1.655420785321498 >5k 2.4961572881014734E-4 1.1290588617116415 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9757 0.6408711170547121 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7356 0.48316572071891595 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4441 0.29169915248949235 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2999 0.1969839581886934 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2980 0.1957359771264776 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2608 0.17130182159256832 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2081 0.1366867679195302 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1909 0.12538925514578717 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1878 0.1233530755179614 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1868 0.12269624337995308 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1685 0.11067621525440094 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 6.568321380083142E-5 0.0 5 0.0 0.0 0.0 6.568321380083142E-5 0.0 6 0.0 0.0 0.0 1.3136642760166285E-4 0.0 7 0.0 0.0 0.0 1.3136642760166285E-4 0.0 8 0.0 0.0 0.0 1.3136642760166285E-4 0.0 9 0.0 0.0 0.0 1.3136642760166285E-4 0.0 10-11 0.0 0.0 0.0 1.3136642760166285E-4 0.0 12-13 0.0 0.0 0.0 1.9704964140249424E-4 0.0 14-15 0.0 0.0 0.0 1.9704964140249424E-4 0.0 16-17 0.0 0.0 0.0 1.9704964140249424E-4 0.0 18-19 0.0 0.0 0.0 1.9704964140249424E-4 0.0 20-21 0.0 0.0 0.0 3.940992828049885E-4 0.0 22-23 0.0 0.0 0.0 5.911489242074828E-4 0.0 24-25 0.0 0.0 0.0 8.538817794108084E-4 0.0 26-27 0.0 0.0 0.0 0.0012808226691162126 0.0 28-29 0.0 0.0 0.0 0.002364595696829931 0.0 30-31 0.0 0.0 0.0 0.004203725683253211 0.0 32-33 0.0 0.0 0.0 0.006995262269788546 0.0 34-35 0.0 0.0 0.0 0.011297512773743005 0.0 36-37 0.0 0.0 0.0 0.01793151736762698 0.0 38-39 0.0 0.0 0.0 0.027816841044652107 0.0 40-41 0.0 0.0 0.0 0.03996823559780592 0.0 42-43 0.0 0.0 0.0 0.0524808878268643 0.0 44-45 0.0 0.0 0.0 0.07031388037379004 0.0 46-47 0.0 0.0 0.0 0.09195649932116398 0.0 48-49 0.0 0.0 0.0 0.11655486288957535 0.0 50-51 0.0 0.0 0.0 0.14397760465142245 0.0 52-53 0.0 0.0 0.0 0.17277969390308706 0.0 54-55 0.0 0.0 0.0 0.20627813294151107 0.0 56-57 0.0 0.0 0.0 0.24910358833965315 0.0 58-59 0.0 0.0 0.0 0.2965597103107539 0.0 60-61 0.0 0.0 0.0 0.35193065954485475 0.0 62-63 0.0 0.0 0.0 0.4101916701861922 0.0 64-65 0.0 0.0 0.0 0.46983202831734716 0.0 66-67 0.0 0.0 0.0 0.542182088318963 0.0 68-69 0.0 0.0 0.0 0.6306573773086829 0.0 70-71 0.0 0.0 0.0 0.7352250536796066 0.0 72-73 0.0 0.0 0.0 0.84373372287858 0.0 74-75 0.0 0.0 0.0 0.9521110256499519 0.0 76-77 0.0 0.0 0.0 1.07126037548466 0.0 78-79 0.0 0.0 0.0 1.2188833985020286 0.0 80-81 0.0 0.0 0.0 1.378099508755244 0.0 82-83 0.0 0.0 0.0 1.5466426353681775 0.0 84-85 0.0 0.0 0.0 1.7273371565342646 0.0 86-87 0.0 0.0 0.0 1.9246823723988626 0.0 88-89 0.0 0.0 0.0 2.144327039348843 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9440 0.0 25.72833 1 GTATCAA 15490 0.0 25.529047 1 GTATAGG 550 0.0 25.060976 1 TACATGG 9730 0.0 24.327274 2 ACATGGG 9565 0.0 23.504616 3 GAGTACT 7965 0.0 23.478186 12-13 TCAACGC 17645 0.0 22.25027 4 ATCAACG 17675 0.0 22.212507 3 CAACGCA 17805 0.0 22.101536 5 TATCAAC 17855 0.0 22.0152 2 GTACTTT 8535 0.0 21.715343 14-15 AACGCAG 18150 0.0 21.706896 6 GGTATCA 11295 0.0 21.460836 1 AGTACTT 8265 0.0 21.217249 12-13 CATGGGG 3445 0.0 20.695646 4 AATTCGC 235 0.0 20.216322 78-79 CATGGGA 5350 0.0 19.989674 4 ACGCAGA 19755 0.0 19.89651 7 CGCAGAG 19840 0.0 19.78601 8 CAATTCG 230 0.0 19.623016 78-79 >>END_MODULE