FastQCFastQC Report
Thu 26 May 2016
SRR936421_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936421_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1522459
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94810.6227425500456827No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87590.5753192696814824No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53950.3543609384554855No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT50520.33183159612180035No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA25910.1701852069579542No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23500.15435555243195384No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA22400.1471303989138624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT100900.023.30177312-13
GTACTTT106100.022.00269914-15
GTAAGGT5700.021.6653794
AGTACTT103700.021.57332412-13
GTATCAA197450.020.6950281
GTACATG89950.020.4953461
GTATAGG4950.020.1575981
GGTATCA156200.019.5289441
TACATGG92750.019.5128922
ACTTTTT122700.019.31597516-17
TACTTTT116350.019.00295314-15
TGTACCG1507.727427E-418.9982455
TAAGGTG6600.018.7103945
ACATGGG92200.018.2879583
GGTAAGG6800.018.1606853
TATCAAC230300.017.6765482
TCAACGC232850.017.4608484
ATCAACG233600.017.4251213
CAACGCA234200.017.3190675
AACGCAG237750.017.100986
Produced by FastQC (version 0.11.5)