FastQCFastQC Report
Thu 26 May 2016
SRR936420_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936420_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1449868
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93420.6443345187286015No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69160.47700894150364037No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44010.3035448744299481No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT30340.20926042922528118No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25300.1744986440144896No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA20970.1446338563234722No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC19420.13394322793523272No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA18620.1284254842509801No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18450.1272529637180764No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT18420.12704604832991692No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT15350.10587170694159745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG88150.026.4212441
TACATGG88950.025.7026922
GTATCAA136750.025.2689131
ACATGGG89850.023.9127673
GAGTACT74600.023.3188312-13
GTACACG2052.6519047E-823.185991
GTGTAGC7600.022.5148161
GGTATCA100800.022.4924811
ATCAACG156950.021.9576663
TCAACGC157200.021.9235024
CATGGGA49700.021.7117064
CAACGCA159550.021.6311325
TATCAAC159850.021.5875572
GTACTTT80100.021.35794614-15
AACGCAG162850.021.221996
GTATAGG5600.020.3705481
AGTACTT78400.020.36984612-13
ACGCAGA175550.019.6325477
CATGGGG35200.019.5816734
CGCAGAG177050.019.4923928
Produced by FastQC (version 0.11.5)