##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936420_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1449868 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07747946709632 33.0 31.0 34.0 31.0 34.0 2 32.18539204948313 33.0 31.0 34.0 31.0 34.0 3 32.16537850342238 34.0 31.0 34.0 30.0 34.0 4 35.80489258332483 37.0 35.0 37.0 35.0 37.0 5 35.84600046349047 37.0 35.0 37.0 35.0 37.0 6 35.933038731801794 37.0 36.0 37.0 35.0 37.0 7 35.84419754074164 37.0 35.0 37.0 35.0 37.0 8 35.841608339517805 37.0 35.0 37.0 35.0 37.0 9 37.466696968275734 39.0 37.0 39.0 35.0 39.0 10-11 37.37799785911545 39.0 37.0 39.0 35.0 39.0 12-13 37.313209202492914 39.0 37.0 39.0 35.0 39.0 14-15 38.46289075971053 40.0 38.0 41.0 34.0 41.0 16-17 38.39163737664394 40.0 38.0 41.0 34.0 41.0 18-19 38.31961461319237 40.0 38.0 41.0 34.0 41.0 20-21 38.310675868423886 40.0 38.0 41.0 34.0 41.0 22-23 38.31526697602816 40.0 38.0 41.0 34.0 41.0 24-25 38.429000433142875 40.0 38.0 41.0 34.0 41.0 26-27 38.363968650939256 40.0 38.0 41.0 34.0 41.0 28-29 38.25108251233905 40.0 38.0 41.0 34.0 41.0 30-31 38.04926655392077 40.0 38.0 41.0 34.0 41.0 32-33 37.8562631218842 40.0 37.0 41.0 33.5 41.0 34-35 37.665776125826625 40.0 37.0 41.0 33.0 41.0 36-37 37.47581538457294 40.0 37.0 41.0 33.0 41.0 38-39 37.287900346790195 39.5 36.0 41.0 33.0 41.0 40-41 37.047664683957436 39.0 36.0 41.0 31.5 41.0 42-43 36.77326935969344 39.0 36.0 41.0 31.0 41.0 44-45 36.58668961588228 39.0 36.0 41.0 31.0 41.0 46-47 36.337434511279646 39.0 36.0 41.0 30.0 41.0 48-49 36.04589141908091 39.0 36.0 41.0 29.5 41.0 50-51 35.068728325613094 38.0 34.0 40.0 27.5 40.5 52-53 35.16861672924708 38.5 35.0 39.5 28.0 40.5 54-55 35.60075606882833 39.0 35.0 40.0 28.0 41.0 56-57 35.46695561251093 39.0 35.0 41.0 28.0 41.0 58-59 35.267799551407435 38.5 35.0 40.0 27.5 41.0 60-61 34.99987895449793 38.0 35.0 40.0 27.0 41.0 62-63 34.682844576195905 38.0 34.5 40.0 26.0 41.0 64-65 34.345414892941974 37.0 34.0 40.0 26.0 41.0 66-67 33.99632656214221 37.0 34.0 39.5 26.0 41.0 68-69 33.583026868652865 36.0 34.0 39.0 25.0 41.0 70-71 33.191117053414516 36.0 33.0 39.0 24.0 40.5 72-73 32.78496490715017 35.0 33.0 38.5 23.0 40.0 74-75 32.38018529962727 35.0 33.0 37.0 22.0 39.5 76-77 31.941220511108597 35.0 32.0 37.0 20.5 39.0 78-79 31.500515564175497 35.0 32.0 36.0 19.5 39.0 80-81 31.074040188486123 35.0 31.0 36.0 18.0 37.5 82-83 30.689033415455754 34.5 31.0 35.5 15.0 37.0 84-85 30.3341307622487 34.0 31.0 35.0 10.0 37.0 86-87 30.023164522563434 34.0 31.0 35.0 7.0 36.0 88-89 29.696228898079 34.0 30.0 35.0 3.5 36.0 90-91 29.37890794196437 34.0 30.0 35.0 2.0 36.0 92-93 29.02557301768161 34.0 29.5 35.0 2.0 35.0 94-95 28.610811122115944 34.0 29.0 35.0 2.0 35.0 96-97 28.10687352227927 34.0 29.0 35.0 2.0 35.0 98-99 27.47222988575512 34.0 28.0 35.0 2.0 35.0 100-101 24.8276684498175 31.5 21.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.47687382195120875 1101 2 0.4217673864222675 1101 3 0.5299255456918885 1101 4 0.5089251707751572 1101 5 0.3287109473293981 1101 6 0.33601903612970574 1101 7 0.39488878444991826 1101 8 0.36276402288790877 1101 9 0.4979960006846511 1101 10-11 0.4868291931406006 1101 12-13 0.5532965839170529 1101 14-15 0.7905551424672339 1101 16-17 0.824854552229354 1101 18-19 1.0192408378354116 1101 20-21 0.8939456967677373 1101 22-23 0.6373461527378979 1101 24-25 0.552442525620755 1101 26-27 0.5637921692467316 1101 28-29 0.5737141851278338 1101 30-31 0.44480445020235493 1101 32-33 0.33278066024085007 1101 34-35 0.4567067294073297 1101 36-37 0.5260801110472642 1101 38-39 0.49552539754607494 1101 40-41 0.5220752350219087 1101 42-43 0.6421320547572051 1101 44-45 0.5556274647808834 1101 46-47 0.5992586106634619 1101 48-49 0.6847932084709143 1101 50-51 0.5209631800439851 1101 52-53 0.44027127403842314 1101 54-55 0.5318251165652086 1101 56-57 0.6630398562624649 1101 58-59 0.49584063104590115 1101 60-61 0.4690850002566833 1101 62-63 0.5552673145020535 1101 64-65 0.40779321980084404 1101 66-67 0.44565721536777403 1101 68-69 0.3868708751620744 1101 70-71 0.31652448212746975 1101 72-73 0.4421953718618781 1101 74-75 0.49584310745333227 1101 76-77 0.48295269883541536 1101 78-79 0.41293830033079004 1101 80-81 0.5075974608095173 1101 82-83 0.5136105948743861 1101 84-85 0.543377493932848 1101 86-87 0.5605006964316779 1101 88-89 0.3561062165717175 1101 90-91 0.3172927734770603 1101 92-93 0.5242063133635142 1101 94-95 0.6076093075576772 1101 96-97 0.4496463994494704 1101 98-99 0.5735710109447432 1101 100-101 1.3017648875236212 1102 1 0.09018120430454246 1102 2 0.14804292045207035 1102 3 0.23126078698930286 1102 4 0.1821783162474091 1102 5 0.10519328865483857 1102 6 0.1191755825812919 1102 7 0.1415358377795144 1102 8 0.12237822721351677 1102 9 0.23863363427549444 1102 10-11 0.26638829332872405 1102 12-13 0.2610942196426862 1102 14-15 0.3032235903451195 1102 16-17 0.20386628020438025 1102 18-19 0.3226703365612096 1102 20-21 0.37898885945168814 1102 22-23 0.14987884163301857 1102 24-25 0.3276775187494181 1102 26-27 0.2150210048549397 1102 28-29 0.0021775402092814033 1102 30-31 -0.0388671131273739 1102 32-33 0.17169912479675276 1102 34-35 -0.09010627009899963 1102 36-37 0.0542074042733276 1102 38-39 -0.026417023300098208 1102 40-41 0.13988034249498327 1102 42-43 0.06618174187777726 1102 44-45 0.400130560219111 1102 46-47 0.17578064564725793 1102 48-49 0.339575460402223 1102 50-51 0.21775883442229826 1102 52-53 0.11449224429819793 1102 54-55 0.3769219490779818 1102 56-57 0.21580623740164384 1102 58-59 0.07836274608413163 1102 60-61 0.18422095937554417 1102 62-63 0.20950569660187313 1102 64-65 0.1364929022515824 1102 66-67 0.10399538532333708 1102 68-69 0.2824888954158524 1102 70-71 0.07528010261606966 1102 72-73 0.3117940322842898 1102 74-75 0.21330798356909497 1102 76-77 0.21253231297302833 1102 78-79 0.18060998374217618 1102 80-81 0.3607442673046002 1102 82-83 0.14721494583303496 1102 84-85 0.154989556802267 1102 86-87 0.030222940891242445 1102 88-89 0.1424449268947363 1102 90-91 0.2458561966047874 1102 92-93 0.3726797753176463 1102 94-95 0.4201041373752865 1102 96-97 0.4603540139606679 1102 98-99 0.4447465769963266 1102 100-101 1.088879792325553 1103 1 0.2670162990782856 1103 2 0.31443764020755083 1103 3 0.31473705402824237 1103 4 0.3748052239773614 1103 5 0.3767981532730005 1103 6 0.34474634794032966 1103 7 0.36817549867613053 1103 8 0.31253126322485514 1103 9 0.33385979765643015 1103 10-11 0.2730795793940146 1103 12-13 0.3453018100936234 1103 14-15 0.6585980468241672 1103 16-17 0.6850959774036767 1103 18-19 0.7514804022759165 1103 20-21 0.6697910879248852 1103 22-23 0.467120076938194 1103 24-25 0.3304944313614939 1103 26-27 0.2844490015657257 1103 28-29 0.15142568405975254 1103 30-31 0.26784127526651247 1103 32-33 0.37879788378416634 1103 34-35 0.3352251620917599 1103 36-37 0.12224931691025631 1103 38-39 0.15148696701123043 1103 40-41 0.23360607483757434 1103 42-43 0.4084346356017221 1103 44-45 0.32730148705594786 1103 46-47 0.4836344923288536 1103 48-49 0.7247711080584622 1103 50-51 0.5531678137414033 1103 52-53 0.6494451283772236 1103 54-55 0.7008161890733717 1103 56-57 0.7420389225368353 1103 58-59 0.677092898185478 1103 60-61 0.6900128817071121 1103 62-63 0.7274149170439372 1103 64-65 0.6887783003430812 1103 66-67 0.6369720555596246 1103 68-69 0.7828642517557967 1103 70-71 0.8903099670602259 1103 72-73 0.9606394759554888 1103 74-75 0.7967738102330202 1103 76-77 0.6035472268001634 1103 78-79 0.6113425699423907 1103 80-81 0.8185482487664721 1103 82-83 0.6830174775742641 1103 84-85 0.6777918330028854 1103 86-87 0.5823775857146565 1103 88-89 0.5249573981712743 1103 90-91 0.6198910833664932 1103 92-93 0.5654687574407369 1103 94-95 0.5022659114833523 1103 96-97 0.3881538491021779 1103 98-99 0.5697795138937032 1103 100-101 1.2906216446071745 1104 1 0.07334187054382824 1104 2 0.14139192867055073 1104 3 0.2744984008464115 1104 4 0.26418576831849805 1104 5 0.21129712626809294 1104 6 0.20343792455314968 1104 7 0.1995879182688185 1104 8 0.1610867260215656 1104 9 0.274388173974927 1104 10-11 0.31877147926413585 1104 12-13 0.36315665715733303 1104 14-15 0.3120057931165334 1104 16-17 0.32105538192544714 1104 18-19 0.31550129349261624 1104 20-21 0.38142593405540737 1104 22-23 0.19293427078716974 1104 24-25 0.3613436850332121 1104 26-27 -0.008155589627911297 1104 28-29 -0.08707929621751731 1104 30-31 -0.04699858480973518 1104 32-33 -0.016188976125242505 1104 34-35 0.05808623429226856 1104 36-37 0.11125621877262404 1104 38-39 0.026214187189737004 1104 40-41 0.1176676434377768 1104 42-43 0.21980728413979733 1104 44-45 0.14594322900666157 1104 46-47 0.19290684114060497 1104 48-49 0.16235391546927502 1104 50-51 0.16333527982372686 1104 52-53 0.07362819875768878 1104 54-55 0.1913002391953711 1104 56-57 0.2583199462588155 1104 58-59 0.19330762562720594 1104 60-61 0.2951336063942662 1104 62-63 0.2884820152574221 1104 64-65 0.4077079192049311 1104 66-67 0.3333105875283593 1104 68-69 0.27770942224800876 1104 70-71 0.28111814724915263 1104 72-73 0.4197415524221171 1104 74-75 0.38179266355241026 1104 76-77 0.3639283011758643 1104 78-79 0.25383089927948177 1104 80-81 0.21592009637834764 1104 82-83 0.0806563565326961 1104 84-85 0.2012493031838254 1104 86-87 0.2452700638897518 1104 88-89 0.25995606049696107 1104 90-91 0.4584262410391844 1104 92-93 0.3801265125446278 1104 94-95 0.5749988052807389 1104 96-97 0.625736220132417 1104 98-99 0.3595008043314998 1104 100-101 0.9813228108510543 1105 1 0.2214623729351075 1105 2 0.23465785016246343 1105 3 0.37767908910732473 1105 4 0.3834194600279446 1105 5 0.29798643927931323 1105 6 0.19587867559578598 1105 7 0.3110921696157334 1105 8 0.2761361159111644 1105 9 0.27070138117179 1105 10-11 0.31897106736655445 1105 12-13 0.43135837007366007 1105 14-15 0.6485450282522862 1105 16-17 0.6030505065433758 1105 18-19 0.41573354459197986 1105 20-21 0.3690317019968532 1105 22-23 0.41337305517357237 1105 24-25 0.30595325907335535 1105 26-27 0.24258671995541192 1105 28-29 0.23363574835887135 1105 30-31 0.13402971509845685 1105 32-33 0.3122494919458205 1105 34-35 0.2210713227746055 1105 36-37 0.23990115136651724 1105 38-39 0.1733584097932237 1105 40-41 0.2827639648966809 1105 42-43 0.3490124730866384 1105 44-45 0.4893535553271846 1105 46-47 0.6335437351990336 1105 48-49 0.7291959881599226 1105 50-51 0.7480806287848694 1105 52-53 0.7543434496267025 1105 54-55 0.5849608672188538 1105 56-57 0.6167019589183056 1105 58-59 0.6927513232159868 1105 60-61 0.6144166364201595 1105 62-63 0.7156963085848886 1105 64-65 0.7716417817934129 1105 66-67 0.7764006445682696 1105 68-69 0.654625794661186 1105 70-71 0.7567783695638255 1105 72-73 0.8228407551011756 1105 74-75 0.6786386214205891 1105 76-77 0.7049820782216862 1105 78-79 0.8089235762355678 1105 80-81 0.7397290144079776 1105 82-83 0.7301356395971048 1105 84-85 0.8431280787684052 1105 86-87 0.8299430385260749 1105 88-89 0.879912463991559 1105 90-91 1.0393513946412263 1105 92-93 1.1838750212209526 1105 94-95 1.2772425812607153 1105 96-97 1.3451982089994914 1105 98-99 1.7001655478925919 1105 100-101 1.7783473837393906 1106 1 0.11731446604747475 1106 2 0.08115876758013485 1106 3 0.08800353810406136 1106 4 0.2657136674011795 1106 5 0.24769426569946518 1106 6 0.18883261312296185 1106 7 0.30762735203079217 1106 8 0.3172320063221221 1106 9 0.34763109002984294 1106 10-11 0.5222682443729028 1106 12-13 0.40685373877239783 1106 14-15 0.34224321626027887 1106 16-17 0.2291972786332508 1106 18-19 0.3318038283651177 1106 20-21 0.21916507757806158 1106 22-23 0.16751101415874103 1106 24-25 -0.0036309922917254767 1106 26-27 -0.10637059248978176 1106 28-29 0.17689991306666286 1106 30-31 0.08914109476200593 1106 32-33 0.2192992388269701 1106 34-35 0.15935269366926264 1106 36-37 0.05812165583570561 1106 38-39 0.20383399321927698 1106 40-41 0.23025893567932343 1106 42-43 0.1723848857108976 1106 44-45 0.15250726147351656 1106 46-47 0.4101422157490475 1106 48-49 0.3572431780616938 1106 50-51 0.22485375028929155 1106 52-53 0.38994220282292247 1106 54-55 0.4268673274603998 1106 56-57 0.5653549392361938 1106 58-59 0.5557163876562896 1106 60-61 0.5595082676550973 1106 62-63 0.5768582235929571 1106 64-65 0.33695602497910926 1106 66-67 0.3464880387336393 1106 68-69 0.45743257692002004 1106 70-71 0.29847371320858684 1106 72-73 0.3215880562720841 1106 74-75 0.4843317691579898 1106 76-77 0.48910006771374626 1106 78-79 0.538976558027219 1106 80-81 0.5981010954354851 1106 82-83 0.6499890738340994 1106 84-85 0.7924424103798948 1106 86-87 0.8231773595264649 1106 88-89 0.9411521200151967 1106 90-91 1.00437899085955 1106 92-93 0.9949986183824855 1106 94-95 0.999256184096236 1106 96-97 0.9662925519589542 1106 98-99 1.144735466909296 1106 100-101 1.8162809464885505 1107 1 0.09657181633723155 1107 2 0.16827501473647288 1107 3 0.18621326912368374 1107 4 0.25425219202389826 1107 5 0.2670295912492904 1107 6 0.1695545306975319 1107 7 0.2545808885265899 1107 8 0.35806231978858705 1107 9 0.3712401794591784 1107 10-11 0.40521968429059285 1107 12-13 0.3303000162948493 1107 14-15 0.20399795556262035 1107 16-17 0.29555062681085076 1107 18-19 0.5310772690351442 1107 20-21 0.3690100538513761 1107 22-23 0.3098131379175939 1107 24-25 0.23762930660306125 1107 26-27 0.1997979577482667 1107 28-29 0.1936612450635451 1107 30-31 0.10462672284369035 1107 32-33 0.16222784380034483 1107 34-35 0.2588521473284118 1107 36-37 0.1895211061459463 1107 38-39 0.2221268708506159 1107 40-41 0.2648777860348943 1107 42-43 0.2868053243879345 1107 44-45 0.19606207507510476 1107 46-47 0.04178446257672164 1107 48-49 0.17652605021793732 1107 50-51 0.2062612224251268 1107 52-53 0.2424105107706822 1107 54-55 0.27181322686671194 1107 56-57 0.5096807437357427 1107 58-59 0.5300967795107923 1107 60-61 0.4580232174563861 1107 62-63 0.4455972081856032 1107 64-65 0.4861316071650421 1107 66-67 0.467342098361506 1107 68-69 0.34490096664367087 1107 70-71 0.4497643704297474 1107 72-73 0.473656168580753 1107 74-75 0.35542940412130974 1107 76-77 0.45278358878116975 1107 78-79 0.42681216044685044 1107 80-81 0.4276637813296098 1107 82-83 0.4534627190217435 1107 84-85 0.41493257196126976 1107 86-87 0.5473313100419261 1107 88-89 0.6743705387498231 1107 90-91 0.6191849444560127 1107 92-93 0.7013931816096566 1107 94-95 0.6153288852006789 1107 96-97 0.8585767629033718 1107 98-99 0.8285390505625294 1107 100-101 1.1939965875687086 1108 1 0.06684354326829123 1108 2 0.15600126878921117 1108 3 0.30513236323120196 1108 4 0.24971360684770616 1108 5 0.16739438258360906 1108 6 0.15624846968771067 1108 7 0.3179266216443395 1108 8 0.42712779857271244 1108 9 0.16476756401694814 1108 10-11 0.3327601696467255 1108 12-13 0.2521051512220325 1108 14-15 0.5305414166269387 1108 16-17 0.6441280126289968 1108 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-2.773000004639833 2316 54-55 -2.20789736666503 2316 56-57 -2.8503854171434284 2316 58-59 -3.1224964869036214 2316 60-61 -3.3187722544610416 2316 62-63 -2.7994044026696656 2316 64-65 -3.5947891282272337 2316 66-67 -3.228366288403791 2316 68-69 -3.6634912734558824 2316 70-71 -3.1391021981116154 2316 72-73 -2.12731497710584 2316 74-75 -2.710335604733128 2316 76-77 -3.048832530721132 2316 78-79 -3.3564656878716477 2316 80-81 -4.219988476078743 2316 82-83 -3.6326201231586595 2316 84-85 -3.3355575281223295 2316 86-87 -4.038959995761573 2316 88-89 -5.2473808504812425 2316 90-91 -4.340831100925886 2316 92-93 -3.7805652393731535 2316 94-95 -3.3646722939874927 2316 96-97 -4.865653237749392 2316 98-99 -7.335642898172264 2316 100-101 -8.059520446436132 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2345.0 3 652.0 4 601.0 5 653.0 6 697.0 7 805.0 8 968.0 9 1265.0 10 1738.0 11 2341.0 12 3828.0 13 5055.0 14 5954.0 15 8274.0 16 11667.0 17 17577.0 18 13080.0 19 7264.0 20 6217.0 21 6176.0 22 6707.0 23 7445.0 24 8347.0 25 9563.0 26 11335.0 27 13845.0 28 16677.0 29 21138.0 30 26918.0 31 35056.0 32 47338.0 33 65579.0 34 96331.0 35 147781.0 36 223010.0 37 312573.0 38 261407.0 39 41643.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.10072965295243 19.05310758170981 14.798662164885556 22.047500600452203 2 15.200319014697167 20.547195754565657 40.55170777039497 23.70077746034221 3 19.3965707537278 25.810196682229066 30.002800679889184 24.790431884153946 4 12.304551957826131 16.831606671550713 38.129700376237054 32.73414099438611 5 13.144457198991164 38.191918829574114 33.896158367319906 14.767465604114813 6 30.365765401547172 36.58026562611031 17.96884540052612 15.085123571816395 7 27.49174170339705 34.093961677086455 20.735606130672274 17.678690488844225 8 26.53020946118933 33.411105797464494 20.03769238678655 20.020992354559628 9 26.76392763331341 16.883213683802246 21.082449822423623 35.27040886046072 10-11 24.595974321505654 26.802454673592933 27.086981500235495 21.514589504665913 12-13 26.261277360276647 24.566292525549137 27.775286263492127 21.39714385068209 14-15 23.150000500221303 25.374260405551833 27.2323238177442 24.243415276482665 16-17 21.33734184901411 28.998176985581487 27.577819669235353 22.086661496169047 18-19 21.046988127216185 27.067007709340015 30.89031733666556 20.995686826778243 20-21 22.099914391416792 25.977307172012548 30.66518765939511 21.25759077717555 22-23 21.574504307133672 26.084267787275607 30.72123105109513 21.61999685449559 24-25 21.59025254756847 26.333008753984377 30.839625723103115 21.23711297534404 26-27 21.282219773131402 26.368498483115932 30.973858542583997 21.375423201168665 28-29 20.815365089875705 26.702982521486017 31.137448440453035 21.344203948185243 30-31 21.015371903220533 26.409194587313333 31.008281529230896 21.567151980235238 32-33 20.765819052184682 26.659780445661507 30.845660128780377 21.72874037337343 34-35 21.641418609375425 26.57955992054993 30.362164162554656 21.416857307519994 36-37 21.54781340766016 26.211345877020005 30.66950703297598 21.57133368234386 38-39 21.313609214399083 26.45076914268923 30.655066353689065 21.580555289222623 40-41 21.420683728142052 26.63051725184437 30.709483134411542 21.239315885602032 42-43 21.11395394471614 26.808668101993007 30.2072049795685 21.870172973722354 44-45 21.24610566116338 26.965653948895792 29.90134916381639 21.886891226124437 46-47 21.586867172967377 27.142702448438232 29.338565092012143 21.93186528658225 48-49 21.714187970757145 27.25117658033525 28.9794831702542 22.055152278653402 50-51 21.546694200483014 27.56485396628004 29.003620020919357 21.88483181231759 52-53 21.808582178340743 27.664817069175406 28.685705220830904 21.840895531652947 54-55 21.285395536825842 27.368996423804976 29.10567535598142 22.239932683387764 56-57 21.758684478434684 27.82581795909584 28.448236618412665 21.967260944056815 58-59 21.824195114212653 27.31985232624106 28.6503646148567 22.20558794468959 60-61 21.629943337207415 27.317570293936082 29.222171257513942 21.83031511134256 62-63 21.60525440007725 27.449895074086143 29.163230924951677 21.78161960088493 64-65 21.363621785421277 27.701684197675707 28.909835746153334 22.024858270749682 66-67 21.26489323290535 27.852315548761037 28.511171584320294 22.371619634013317 68-69 21.398611961793772 27.64509427403202 28.86042090858426 22.095872855589953 70-71 21.485176411514555 27.659156958949456 28.616024794554136 22.239641834981853 72-73 21.46663695666571 27.58028598865317 28.74245982251264 22.21061723216848 74-75 21.325392960173506 28.22221823655285 28.46831415843128 21.984074644842366 76-77 21.438646223513995 27.777971329040636 28.457060307392368 22.326322140053005 78-79 21.493632123224614 27.80273327352293 28.584718130521207 22.118916472731247 80-81 21.562482754814855 28.213605761271452 28.185668561337675 22.038242922576018 82-83 21.504680291046437 28.1009902724715 28.313329466141262 22.080999970340798 84-85 21.426162264169164 28.08188045282832 28.06073991203052 22.431217370972004 86-87 21.591462832151084 28.055414065676516 28.22108654800829 22.13203655416411 88-89 21.60531421591382 27.970537580861983 27.98419419428395 22.439954008940255 90-91 21.344541872515798 28.082080994628523 28.216824879508202 22.356552253347473 92-93 21.592008812572125 28.097488734241217 28.2881771785008 22.02232527468586 94-95 21.31767171299461 27.802664135570026 28.275485772583448 22.604178378851916 96-97 21.425413198484954 27.637513821567783 28.2767376202554 22.66033535969186 98-99 21.240378529309805 27.905240047393775 27.909310255026103 22.945071168270317 100-101 21.31601702933155 27.681666151013047 28.217669983750877 22.78464683590453 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 621.0 1 469.0 2 511.5 3 667.0 4 509.0 5 487.5 6 859.5 7 1701.0 8 2826.5 9 3282.5 10 2730.5 11 2153.5 12 2101.5 13 2272.0 14 2458.0 15 2543.5 16 2676.0 17 2852.0 18 2977.0 19 3192.0 20 3473.0 21 3958.0 22 4629.0 23 5489.0 24 6541.5 25 7930.5 26 10116.5 27 12423.5 28 14257.0 29 16596.0 30 19611.0 31 22896.5 32 27171.5 33 30348.5 34 33178.5 35 35770.5 36 38761.0 37 42255.5 38 42432.5 39 41666.5 40 41893.5 41 43232.5 42 44523.0 43 45978.5 44 47923.5 45 49849.0 46 50025.0 47 52066.5 48 64477.0 49 64009.0 50 51714.0 51 47103.5 52 43182.5 53 41834.0 54 41342.0 55 38640.0 56 35232.0 57 31382.0 58 28529.0 59 25760.5 60 21958.0 61 18236.5 62 15136.5 63 11716.0 64 8513.0 65 6794.0 66 5224.5 67 4029.5 68 2912.0 69 1941.5 70 1521.5 71 1248.5 72 1057.0 73 960.5 74 763.5 75 605.0 76 463.0 77 302.5 78 208.5 79 156.5 80 108.0 81 68.5 82 49.5 83 30.5 84 20.5 85 14.0 86 10.0 87 10.0 88 6.5 89 4.0 90 4.5 91 3.5 92 1.5 93 1.5 94 2.5 95 1.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06621292421103163 2 0.027933577401528964 3 0.01517379513169475 4 0.017242949013289488 5 0.019036215710671593 6 0.026692085072572123 7 0.07048917556632742 8 0.053039311164878464 9 0.06304022159258636 10-11 0.054867063760287146 12-13 0.0347617852107916 14-15 0.035141130089083976 16-17 0.042762513552957925 18-19 0.04048644428320371 20-21 0.017036033625130013 22-23 0.012414923289568428 24-25 0.020657052917920804 26-27 0.02417461451663186 28-29 0.006069518052677899 30-31 0.0010345769407973692 32-33 0.007793812954006847 34-35 0.010518198898106585 36-37 0.0037934487829236873 38-39 0.009449136059282638 40-41 0.0035865333947642133 42-43 0.0016208372072492117 44-45 4.828025723721056E-4 46-47 3.4485898026578973E-4 48-49 6.552320625050005E-4 50-51 0.0010000910427707902 52-53 2.069153881594738E-4 54-55 2.069153881594738E-4 56-57 0.002758871842126318 58-59 0.003483075700684476 60-61 0.00434522315134895 62-63 0.0024484987598871067 64-65 5.862602664518425E-4 66-67 4.4831667434552667E-4 68-69 0.0035865333947642133 70-71 0.002310555167780791 72-73 0.020691538815947385 74-75 0.015863513092226326 76-77 0.024312558108738176 78-79 0.01982939136528291 80-81 0.012966697657993694 82-83 0.004483166743455266 84-85 0.0032416744144984234 86-87 0.00134495002303658 88-89 0.0014484077171163167 90-91 0.005552229582279215 92-93 0.00834558732243211 94-95 0.011518289940877375 96-97 0.009725023243495268 98-99 0.021415742674505543 100-101 0.01734640670736922 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1449868.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.45203897886248 #Duplication Level Percentage of deduplicated Percentage of total 1 77.37248894716743 41.357172950956 2 12.945277328316324 13.839029366906978 3 4.200351269531371 6.735520193517161 4 1.8129513922157532 3.876237939339978 5 0.9349450921837131 2.4987360755250005 6 0.5559846381407497 1.7831107529728865 7 0.37565694016348317 1.4055740585809098 8 0.25518059399136067 1.0911938445340394 9 0.19884827217772227 0.9565961035790759 >10 1.1656061157127469 11.86769877518621 >50 0.11584678025678048 4.281791239776473 >100 0.06076065560288916 5.952577128882137 >500 0.004284365102330153 1.5093018452195885 >1k 0.0015579509463018739 1.717213396476101 >5k 2.5965849105031235E-4 1.1282463285475226 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9342 0.6443345187286015 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6916 0.47700894150364037 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4401 0.3035448744299481 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3034 0.20926042922528118 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2530 0.1744986440144896 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2097 0.1446338563234722 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1942 0.13394322793523272 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1862 0.1284254842509801 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1845 0.1272529637180764 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1842 0.12704604832991692 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1535 0.10587170694159745 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 6.897179605315794E-5 0.0 5 0.0 0.0 0.0 6.897179605315794E-5 0.0 6 0.0 0.0 0.0 6.897179605315794E-5 0.0 7 0.0 0.0 0.0 6.897179605315794E-5 0.0 8 0.0 0.0 0.0 6.897179605315794E-5 0.0 9 0.0 0.0 0.0 1.3794359210631587E-4 0.0 10-11 0.0 0.0 0.0 1.3794359210631587E-4 0.0 12-13 0.0 0.0 0.0 1.3794359210631587E-4 0.0 14-15 0.0 0.0 0.0 1.3794359210631587E-4 0.0 16-17 0.0 0.0 0.0 1.3794359210631587E-4 0.0 18-19 0.0 0.0 0.0 1.3794359210631587E-4 0.0 20-21 0.0 0.0 0.0 1.3794359210631587E-4 0.0 22-23 0.0 0.0 0.0 2.7588718421263174E-4 0.0 24-25 0.0 0.0 0.0 3.4485898026578973E-4 0.0 26-27 0.0 0.0 0.0 4.1383077631894767E-4 0.0 28-29 0.0 0.0 0.0 7.586897565847374E-4 0.0 30-31 0.0 0.0 0.0 0.0020691538815947384 0.0 32-33 0.0 0.0 0.0 0.004586624437535003 0.0 34-35 0.0 0.0 0.0 0.0080352142401929 0.0 36-37 0.0 0.0 0.0 0.013828845108658169 0.0 38-39 0.0 0.0 0.0 0.024278072210711595 0.0 40-41 0.0 0.0 0.0 0.034934214700924504 0.0 42-43 0.0 0.0 0.0 0.04555587129311082 0.0 44-45 0.0 0.0 0.0 0.06007443436230057 0.0 46-47 0.0 0.0 0.0 0.07886924878678611 0.0 48-49 0.0 0.0 0.0 0.09900901323430822 0.0 50-51 0.0 0.0 0.0 0.1224939097904085 0.0 52-53 0.0 0.0 0.0 0.14756515765573142 0.0 54-55 0.0 0.0 0.0 0.17677471328424382 0.0 56-57 0.0 0.0 0.0 0.21491611650164016 0.0 58-59 0.0 0.0 0.0 0.26081684677501676 0.0 60-61 0.0 0.0 0.0 0.31102831430171574 0.0 62-63 0.0 0.0 0.0 0.3630330485257969 0.0 64-65 0.0 0.0 0.0 0.4234523418683632 0.0 66-67 0.0 0.0 0.0 0.49476917898732853 0.0 68-69 0.0 0.0 0.0 0.5784664534978357 0.0 70-71 0.0 0.0 0.0 0.6740268769294859 0.0 72-73 0.0 0.0 0.0 0.7741394389006447 0.0 74-75 0.0 0.0 0.0 0.8828734753784482 0.0 76-77 0.0 0.0 0.0 1.0011256197115874 0.0 78-79 0.0 0.0 0.0 1.1410004221073917 0.0 80-81 0.0 0.0 0.0 1.2957041606546251 0.0 82-83 0.0 0.0 0.0 1.454856580047287 0.0 84-85 0.0 0.0 0.0 1.6301483997163881 0.0 86-87 0.0 0.0 0.0 1.8147169259546385 0.0 88-89 0.0 0.0 0.0 2.0234255808114945 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8815 0.0 26.421244 1 TACATGG 8895 0.0 25.702692 2 GTATCAA 13675 0.0 25.268913 1 ACATGGG 8985 0.0 23.912767 3 GAGTACT 7460 0.0 23.31883 12-13 GTACACG 205 2.6519047E-8 23.18599 1 GTGTAGC 760 0.0 22.514816 1 GGTATCA 10080 0.0 22.492481 1 ATCAACG 15695 0.0 21.957666 3 TCAACGC 15720 0.0 21.923502 4 CATGGGA 4970 0.0 21.711706 4 CAACGCA 15955 0.0 21.631132 5 TATCAAC 15985 0.0 21.587557 2 GTACTTT 8010 0.0 21.357946 14-15 AACGCAG 16285 0.0 21.22199 6 GTATAGG 560 0.0 20.370548 1 AGTACTT 7840 0.0 20.369846 12-13 ACGCAGA 17555 0.0 19.632547 7 CATGGGG 3520 0.0 19.581673 4 CGCAGAG 17705 0.0 19.492392 8 >>END_MODULE