FastQCFastQC Report
Thu 26 May 2016
SRR936420_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936420_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1449868
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92250.6362648185903821No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86050.5935023050374241No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53260.3673437857791192No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT50910.3511354137066271No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA24480.16884295673813063No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23790.16408390281046273No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA18970.13083949711284062No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15250.10518198898106586No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC14990.10338872228368376No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGCG1109.4916926E-525.9037135
TAAGGTG8650.024.1568345
GGTAAGG7000.023.7467063
GTAAGGT7200.023.7450684
GAGTACT97450.022.32040412-13
GTACTTT102400.021.19432814-15
AGTACTT99350.021.12870212-13
ACCGTCG1150.003727478220.6485988
GCACCGT3001.6734703E-1020.579066
GGTATCA147100.020.4120961
AGGTAAG7750.020.2250942
GTATCAA184300.019.926791
CGATCGT1950.019.4851524-25
GTACATG85050.018.9927461
CCGACGG2051.5922347E-518.5333759
ACTTTTT119100.018.42186716-17
TACTTTT114400.018.16165714-15
TACATGG87000.018.1257972
AAGGTGT11250.017.729656
ACATGGG86050.017.3857363
Produced by FastQC (version 0.11.5)