##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936420_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1449868 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.10401981421757 31.0 31.0 33.0 30.0 34.0 2 31.264577878813796 31.0 31.0 34.0 28.0 34.0 3 31.28292989430762 31.0 31.0 34.0 28.0 34.0 4 35.11041625858354 35.0 35.0 37.0 32.0 37.0 5 34.899706042205224 35.0 35.0 37.0 32.0 37.0 6 35.04205072461769 36.0 35.0 37.0 32.0 37.0 7 34.97014417864247 36.0 35.0 37.0 32.0 37.0 8 34.940643562034616 36.0 35.0 37.0 32.0 37.0 9 36.399993654594766 38.0 35.0 39.0 32.0 39.0 10-11 36.24037946902753 38.0 35.0 39.0 32.0 39.0 12-13 36.09463999481332 37.5 35.0 39.0 31.0 39.0 14-15 36.90289943636248 38.0 36.0 40.0 31.0 41.0 16-17 36.76275529910309 38.0 36.0 40.0 31.0 41.0 18-19 36.99481435551374 38.5 36.0 40.0 31.0 41.0 20-21 37.04023538694557 39.0 36.0 40.0 31.0 41.0 22-23 36.917133490772954 39.0 36.0 40.0 31.0 41.0 24-25 36.71619692275435 38.5 35.0 40.0 30.0 41.0 26-27 36.38633068665561 38.0 35.0 40.0 30.0 41.0 28-29 36.213164232881894 38.0 35.0 40.0 30.0 41.0 30-31 36.06436172120497 38.0 35.0 40.0 30.0 41.0 32-33 35.788263828155394 38.0 34.0 40.0 29.0 41.0 34-35 35.50974709421823 38.0 34.0 40.0 28.0 41.0 36-37 35.187493275249885 38.0 34.0 40.0 27.0 41.0 38-39 34.90964729202935 38.0 33.0 40.0 26.0 41.0 40-41 34.60709871519339 38.0 33.0 40.0 25.0 41.0 42-43 34.19187126000436 37.0 33.0 40.0 24.0 41.0 44-45 33.75631160905682 37.0 33.0 40.0 23.5 41.0 46-47 33.156728060761395 37.0 31.5 40.0 20.0 41.0 48-49 32.569993613211686 37.0 31.0 39.0 16.5 40.0 50-51 32.1363465501687 36.0 30.5 39.0 13.5 40.0 52-53 31.688258517327093 36.0 30.0 39.0 9.0 40.0 54-55 31.38042670091346 35.5 30.0 39.0 8.5 40.0 56-57 30.970396615416025 35.0 29.0 38.5 7.5 40.0 58-59 30.484865518792056 35.0 28.0 38.0 6.5 40.0 60-61 29.959330780457258 34.0 28.0 38.0 2.0 40.0 62-63 29.497789109077516 34.0 27.0 38.0 2.0 40.0 64-65 28.939447591091053 34.0 26.0 37.5 2.0 39.0 66-67 28.401412059580593 33.0 26.0 37.0 2.0 39.0 68-69 27.925409416581374 33.0 25.0 36.0 2.0 39.0 70-71 27.419722002278828 32.0 24.5 36.0 2.0 39.0 72-73 26.824800257678632 32.0 22.5 35.0 2.0 38.0 74-75 26.203418862958557 31.0 22.0 35.0 2.0 37.5 76-77 25.134005992269643 30.0 21.0 34.0 2.0 36.0 78-79 25.804868788055188 31.0 22.0 34.0 2.0 36.0 80-81 25.491685449985795 31.0 20.0 34.0 2.0 36.0 82-83 24.993216623858174 31.0 19.0 34.0 2.0 36.0 84-85 24.44793526031335 31.0 17.5 34.0 2.0 35.0 86-87 23.827771907511583 30.0 10.5 34.0 2.0 35.0 88-89 23.095032444332865 29.5 4.5 34.0 2.0 35.0 90-91 22.322070698849828 29.0 2.0 34.0 2.0 35.0 92-93 21.387843238143056 29.0 2.0 33.5 2.0 35.0 94-95 20.480547539500147 27.5 2.0 33.0 2.0 34.5 96-97 19.336252679554278 26.0 2.0 33.0 2.0 34.0 98-99 17.997545638637447 24.5 2.0 32.0 2.0 34.0 100-101 14.968227452430153 9.5 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.37516200721397297 1101 2 0.3829699787778402 1101 3 0.37089309568959905 1101 4 0.2925433964914319 1101 5 0.2055850543940707 1101 6 0.20107237835600955 1101 7 0.2709041046525442 1101 8 0.25933165283753823 1101 9 0.2431562538529164 1101 10-11 0.3641203551258627 1101 12-13 0.45210506625505786 1101 14-15 0.7114284839374747 1101 16-17 0.7897044298582685 1101 18-19 0.6487034972356369 1101 20-21 0.5739192799753141 1101 22-23 0.4668460639070844 1101 24-25 0.5554048758936716 1101 26-27 0.6111555408122271 1101 28-29 0.6190079681234266 1101 30-31 0.7010195656843194 1101 32-33 0.802761764241616 1101 34-35 0.8489242182549503 1101 36-37 1.0513131127559987 1101 38-39 0.8757999574343671 1101 40-41 1.0312746663480539 1101 42-43 1.014705139308056 1101 44-45 1.1099826071993633 1101 46-47 1.1464212411361672 1101 48-49 1.211628164289067 1101 50-51 1.2947482515687057 1101 52-53 1.3327152472128745 1101 54-55 1.514100833166463 1101 56-57 1.462111639199854 1101 58-59 1.4008063710962766 1101 60-61 1.5420940500391964 1101 62-63 1.5200169419750011 1101 64-65 1.7636462015248 1101 66-67 1.4595033405907536 1101 68-69 1.325819041427625 1101 70-71 1.2283968943277266 1101 72-73 1.488450154714151 1101 74-75 1.3836391899656029 1101 76-77 1.3726420720062364 1101 78-79 1.5770477910638299 1101 80-81 1.4491338588169143 1101 82-83 1.797868113431793 1101 84-85 1.5803183828524716 1101 86-87 1.5298141420741977 1101 88-89 1.4834314804341702 1101 90-91 1.7226590193178204 1101 92-93 1.6756514769381035 1101 94-95 1.8302717687942192 1101 96-97 2.1998989563056206 1101 98-99 1.8967853057813961 1101 100-101 2.021651483023911 1102 1 0.23513378802492468 1102 2 0.26291734298550296 1102 3 0.1977280100980643 1102 4 0.2180227224330693 1102 5 0.24799289172345595 1102 6 0.09477342840337144 1102 7 0.3388483067099415 1102 8 0.30740434226635216 1102 9 0.23335825788232256 1102 10-11 0.232896289427849 1102 12-13 0.41587693112317226 1102 14-15 0.6434942886846997 1102 16-17 0.5930553751677508 1102 18-19 0.31610166132054474 1102 20-21 0.14974801017852002 1102 22-23 0.40667314632312923 1102 24-25 0.40492454927156984 1102 26-27 0.4804381529885049 1102 28-29 0.5371012715697745 1102 30-31 0.7648718185495937 1102 32-33 0.8705715415568633 1102 34-35 0.6142067770616109 1102 36-37 0.7835348568753417 1102 38-39 0.8085594005223342 1102 40-41 0.8529937686709701 1102 42-43 1.0364671041107911 1102 44-45 1.00447185989119 1102 46-47 1.1102192904763797 1102 48-49 1.1715000438338237 1102 50-51 1.165078909749596 1102 52-53 1.1006589489300467 1102 54-55 1.0173865674657812 1102 56-57 0.8285933467609929 1102 58-59 1.0882504997704388 1102 60-61 1.3522379478897335 1102 62-63 1.3325844879062352 1102 64-65 1.1553694066376075 1102 66-67 0.8689669425067237 1102 68-69 1.1675776039167864 1102 70-71 1.2310967302161302 1102 72-73 1.444380187136197 1102 74-75 1.4227145945740673 1102 76-77 1.1549403681809665 1102 78-79 1.2837998824859973 1102 80-81 1.2025920437159776 1102 82-83 1.4783473393053903 1102 84-85 1.4248580031275893 1102 86-87 1.3764682001670359 1102 88-89 1.2777456140450276 1102 90-91 1.2400995455423178 1102 92-93 1.2173486643588838 1102 94-95 1.3457978265134685 1102 96-97 1.3870175302692473 1102 98-99 1.2831797206501712 1102 100-101 1.5887782959233494 1103 1 0.10086062207566115 1103 2 0.17075866121708216 1103 3 0.13079327437378296 1103 4 0.21237398238747573 1103 5 0.22030601149643303 1103 6 0.19931240630060643 1103 7 0.20436036733173069 1103 8 0.11344206085549047 1103 9 0.4573361203099964 1103 10-11 0.27394983264255046 1103 12-13 0.35746004993166025 1103 14-15 0.3180518902223213 1103 16-17 0.25395348605646006 1103 18-19 0.21639558273534476 1103 20-21 0.10561405917271571 1103 22-23 0.05463911268920896 1103 24-25 0.24645655995853843 1103 26-27 0.312503229337473 1103 28-29 0.16305395579018978 1103 30-31 0.3153977648701556 1103 32-33 0.41937234019584224 1103 34-35 0.3548961997685325 1103 36-37 0.24640987763179822 1103 38-39 0.3464828198744101 1103 40-41 0.42752263431924575 1103 42-43 0.46388611414448633 1103 44-45 0.5761505031594893 1103 46-47 0.5350983064163515 1103 48-49 0.6087126227953732 1103 50-51 0.8306498342000594 1103 52-53 1.0010970469522391 1103 54-55 0.9651469081600759 1103 56-57 0.6901917582330768 1103 58-59 0.8776949823305387 1103 60-61 1.06389318483156 1103 62-63 1.0748195832765752 1103 64-65 1.1287156263766782 1103 66-67 1.0493269067509239 1103 68-69 1.11071434996013 1103 70-71 1.1535033659196294 1103 72-73 1.0708867083932958 1103 74-75 0.9424439203943074 1103 76-77 0.8677191883390449 1103 78-79 0.5372255683937794 1103 80-81 0.6818355838582235 1103 82-83 0.7725203174273325 1103 84-85 1.176489436014375 1103 86-87 1.2741664454880102 1103 88-89 1.265911697542176 1103 90-91 1.4250900646874811 1103 92-93 1.2818343864003054 1103 94-95 1.2601484699885823 1103 96-97 1.4172682683043298 1103 98-99 1.3893339737109436 1103 100-101 1.537212289386682 1104 1 0.0872841509108504 1104 2 0.21412851289132462 1104 3 0.21888777158111594 1104 4 0.2441163056978155 1104 5 0.07191445995871248 1104 6 -0.03610719544505514 1104 7 0.12436207257276521 1104 8 0.07273110961632057 1104 9 0.16903155620551757 1104 10-11 0.19209641690714108 1104 12-13 0.34694260662504917 1104 14-15 0.27851765599806555 1104 16-17 0.43236013300543874 1104 18-19 0.18294231187828558 1104 20-21 0.20578572457890942 1104 22-23 0.28885598242389676 1104 24-25 0.3800950911552192 1104 26-27 0.19924031440248058 1104 28-29 0.3361778801540254 1104 30-31 0.43014051348372817 1104 32-33 0.45470053699407487 1104 34-35 0.48443409623287437 1104 36-37 0.4207712766142251 1104 38-39 0.5871861512373187 1104 40-41 0.7546598669480318 1104 42-43 0.5582069230195259 1104 44-45 0.6966888893771142 1104 46-47 0.8822143846573738 1104 48-49 1.0404197335497756 1104 50-51 0.9831242295695972 1104 52-53 0.9815171538923337 1104 54-55 1.0731975927572854 1104 56-57 0.9360594859087712 1104 58-59 1.0299328929940366 1104 60-61 1.046517181533865 1104 62-63 0.9512072315578557 1104 64-65 1.156094311736549 1104 66-67 1.061610180853986 1104 68-69 1.0596235831353944 1104 70-71 1.1473319375016509 1104 72-73 1.311687050515669 1104 74-75 1.3249677509698863 1104 76-77 1.1263253686293453 1104 78-79 0.883981800495885 1104 80-81 1.1854291779255988 1104 82-83 1.2204883112616152 1104 84-85 1.7549775182929217 1104 86-87 1.9064965063026769 1104 88-89 1.8117539808733412 1104 90-91 1.8891799711711208 1104 92-93 1.8302551754368146 1104 94-95 1.8139026688928652 1104 96-97 2.1084492853463352 1104 98-99 2.2911966809959132 1104 100-101 2.4479330189075927 1105 1 -0.027717447856897337 1105 2 0.0943811525772098 1105 3 0.07032202096825557 1105 4 0.17969765547037753 1105 5 0.033048244156013595 1105 6 0.02626847027839574 1105 7 0.02827991310169864 1105 8 0.1357731004775573 1105 9 0.19348651393272576 1105 10-11 0.22956173396169532 1105 12-13 0.18112151045487224 1105 14-15 0.3073965587964196 1105 16-17 0.1939565283858613 1105 18-19 0.32311178125930695 1105 20-21 0.28973908181927044 1105 22-23 0.3000578431832679 1105 24-25 0.3351351634951598 1105 26-27 0.3586011855074176 1105 28-29 0.2844029041355469 1105 30-31 0.135330491788622 1105 32-33 0.28761452328937054 1105 34-35 0.44574374488217927 1105 36-37 0.4456178464838203 1105 38-39 0.39836256924115787 1105 40-41 0.6819386267168426 1105 42-43 0.6818666227262469 1105 44-45 0.9303108031536809 1105 46-47 0.8251194659882373 1105 48-49 0.9003804004250284 1105 50-51 1.0353685212811001 1105 52-53 1.0270096603131904 1105 54-55 0.7012808937141379 1105 56-57 0.6899652927019844 1105 58-59 0.8053612080303587 1105 60-61 0.8528240624239487 1105 62-63 0.9242449590384361 1105 64-65 0.8384415090794981 1105 66-67 0.8368547235548363 1105 68-69 0.8430802909115975 1105 70-71 0.9795115220970949 1105 72-73 1.0800354030972485 1105 74-75 1.1089092463850285 1105 76-77 0.9027198207333598 1105 78-79 0.9971644141545148 1105 80-81 1.0917804117273278 1105 82-83 1.4656901170096113 1105 84-85 1.5997555646331207 1105 86-87 1.8492754053190055 1105 88-89 1.487993197786583 1105 90-91 1.7487082831130571 1105 92-93 1.5977290192526787 1105 94-95 1.6555005427036704 1105 96-97 2.0031809158487626 1105 98-99 2.007460274819511 1105 100-101 2.3335356855834295 1106 1 0.16215337678737285 1106 2 0.22399274225202248 1106 3 0.10721473836676765 1106 4 0.2515126686974014 1106 5 0.30979962765133706 1106 6 0.004440561622246264 1106 7 0.03532965998284254 1106 8 0.17459128966455495 1106 9 0.1988355379329647 1106 10-11 0.205069629648726 1106 12-13 0.31091179393324353 1106 14-15 0.47015013819910934 1106 16-17 0.25658299570147847 1106 18-19 0.35016676000027047 1106 20-21 0.15959472670125763 1106 22-23 0.07462068813599387 1106 24-25 0.12217194125901898 1106 26-27 0.05374329313635684 1106 28-29 -0.15585736246823956 1106 30-31 0.09164519920975778 1106 32-33 0.1359027471835006 1106 34-35 0.1772399094131174 1106 36-37 -0.07133632684548985 1106 38-39 0.3012651461163429 1106 40-41 0.35329456266708803 1106 42-43 0.5366298258034661 1106 44-45 0.15714803336287986 1106 46-47 0.32375623490951 1106 48-49 -0.17220318037773552 1106 50-51 0.18407787304629863 1106 52-53 -0.1859904060061588 1106 54-55 -0.03348923605071619 1106 56-57 -0.03802773639747414 1106 58-59 0.021697506084255025 1106 60-61 0.08518753667362233 1106 62-63 0.18598974744287133 1106 64-65 0.31348148490977934 1106 66-67 0.41140283087428386 1106 68-69 0.23737056333674644 1106 70-71 0.1293670932909201 1106 72-73 0.4805799586470769 1106 74-75 0.4664438614012063 1106 76-77 0.2850700311130723 1106 78-79 -0.16990802868189192 1106 80-81 0.3154328393839734 1106 82-83 0.7305909695816979 1106 84-85 0.9333231035220493 1106 86-87 1.0081330913632023 1106 88-89 1.1036651970202271 1106 90-91 1.211194742912479 1106 92-93 1.170472134935732 1106 94-95 1.1537581860087371 1106 96-97 1.4407935666331717 1106 98-99 1.356221576742037 1106 100-101 1.7116392469325117 1107 1 0.006789696031109571 1107 2 0.0813537796999313 1107 3 0.08930503920080568 1107 4 0.10399168539292702 1107 5 0.04779784727334402 1107 6 -0.014367504128685482 1107 7 0.053930560140713624 1107 8 0.08665105304406495 1107 9 0.13796623683646203 1107 10-11 0.1223673336153226 1107 12-13 -0.11691572435535136 1107 14-15 -0.08420396075906922 1107 16-17 0.19354040292283514 1107 18-19 0.04337204007490669 1107 20-21 0.17273085552398726 1107 22-23 0.04210094704625078 1107 24-25 0.07271874939019085 1107 26-27 0.13031668076888536 1107 28-29 0.17361731698973415 1107 30-31 0.14762663841872836 1107 32-33 0.3753809157461987 1107 34-35 0.28093641337690656 1107 36-37 0.322834376045563 1107 38-39 0.34310928593910006 1107 40-41 0.18045693142651942 1107 42-43 0.17739748336030203 1107 44-45 0.4299932970200402 1107 46-47 0.09338809542009585 1107 48-49 0.23515213275773306 1107 50-51 0.48699213219176585 1107 52-53 0.6434357920301323 1107 54-55 0.4922945079922947 1107 56-57 0.21797558759783442 1107 58-59 0.4977819517644271 1107 60-61 0.6674365606199366 1107 62-63 0.8549155704782798 1107 64-65 0.8494580097770488 1107 66-67 1.0809351584420277 1107 68-69 0.9223221247500497 1107 70-71 0.9560689858965858 1107 72-73 1.057446765968276 1107 74-75 0.8609994950981665 1107 76-77 0.5453979368636297 1107 78-79 0.6300527578308497 1107 80-81 0.9976807340441596 1107 82-83 1.022162912506797 1107 84-85 1.156808049359153 1107 86-87 1.0990998148057045 1107 88-89 1.0580369751474343 1107 90-91 1.0982127811166187 1107 92-93 1.2808242229857818 1107 94-95 1.4092071862789837 1107 96-97 1.8453466925361148 1107 98-99 1.5921454145703215 1107 100-101 1.691261668168698 1108 1 0.06213138631498438 1108 2 0.0991936244205398 1108 3 0.05827796973920485 1108 4 0.019897619884133633 1108 5 0.031172773300767176 1108 6 0.06876866903136403 1108 7 0.05479242318131128 1108 8 -0.01583982717257726 1108 9 0.25228672745019765 1108 10-11 0.17048761992671047 1108 12-13 0.19144038592951063 1108 14-15 0.12486864820118626 1108 16-17 0.24770633436510536 1108 18-19 0.29667214726762126 1108 20-21 0.1744197709809825 1108 22-23 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8 1581.0 9 2818.0 10 4411.0 11 6538.0 12 9081.0 13 12057.0 14 15552.0 15 20358.0 16 24071.0 17 29104.0 18 25287.0 19 19532.0 20 19143.0 21 20113.0 22 21477.0 23 24186.0 24 27545.0 25 32353.0 26 38515.0 27 45119.0 28 52504.0 29 60979.0 30 71121.0 31 81878.0 32 94566.0 33 109416.0 34 126982.0 35 146983.0 36 152733.0 37 118213.0 38 33746.0 39 691.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.98183446202538 22.5833377598021 16.467015679357182 19.967812098815337 2 17.405292056397098 23.69581712330657 37.09626686532572 21.802623954970617 3 21.114176731481546 26.514067748677377 30.91793870751912 21.453816812321953 4 14.572499013703316 19.465220282122235 36.38731250017243 29.574968204002015 5 12.843901914152811 39.26648053015798 33.78763884367292 14.101978712016288 6 29.938035738425846 38.636137910485644 16.461429592211154 14.96439675887736 7 26.70815550105251 35.038293141168715 20.528213602893505 17.725337754885274 8 26.67953220569045 34.692330612166074 19.180504708014798 19.447632474128678 9 26.357502889918255 17.00865182209691 20.57070022926225 36.06314505872259 10-11 24.961134392924045 27.77621824883369 26.44554538758011 20.817101970662158 12-13 26.71346208626092 25.229143287924256 27.558614401282334 20.49878022453249 14-15 23.131262447301037 26.311322625421376 27.087982481097345 23.469432446180242 16-17 20.966440370047735 29.740727942631977 27.71847348756817 21.574358199752115 18-19 20.875951174343594 27.335125469659843 31.43116282518394 20.357760530812616 20-21 21.969068908342003 25.975468111579815 31.651191694692205 20.40427128538598 22-23 21.605077705376527 26.099591859256105 31.556469815345196 20.738860620022173 24-25 21.67293850198777 26.5320705057288 31.630741557162445 20.16424943512099 26-27 21.409305868854574 26.418622359508596 31.834774460917814 20.337297310719016 28-29 20.820068842577697 26.854743954375326 31.969655742278665 20.35553146076832 30-31 21.10470239111355 26.475956954093448 31.7819287890623 20.637411865730705 32-33 20.96301530920708 26.692146948246542 31.62110531585306 20.72373242669332 34-35 21.716615230009626 26.385884250105963 31.372049979256357 20.52545054062806 36-37 21.849357349528056 26.1313372716582 31.413210286545894 20.606095092267847 38-39 21.378684597679904 26.470959469684168 31.659106130069738 20.49124980256619 40-41 21.427667401334542 26.5958851931719 31.36685814472624 20.609589260767322 42-43 21.339114876264496 26.868068804637375 30.855691805549235 20.937124513548895 44-45 21.773705452427354 26.74749394847226 30.349579833091983 21.129220766008398 46-47 22.1165763709035 27.270573467184878 29.58089201408407 21.031958147827556 48-49 22.28420536791055 27.451785745692764 29.15447149424788 21.10953739214881 50-51 21.989457657337653 27.66187255042271 29.120109251362624 21.228560540877012 52-53 22.401679605232076 27.918643431851738 28.588119786366246 21.091557176549948 54-55 21.825703646123955 27.672275787482903 28.854232981409684 21.64778758498346 56-57 22.27513570601273 27.950678823239684 28.443387017889254 21.33079845285833 58-59 22.49434717893615 27.439785166928537 28.5261616525727 21.53970600156261 60-61 22.028638976022428 27.67178798023956 29.066242722806805 21.233330320931206 62-63 22.12033605190745 27.58128302074215 29.12840761642237 21.169973310928032 64-65 21.973055810991944 27.830060796543023 28.75615088138776 21.44073251107728 66-67 21.86096702249208 27.95371706103131 28.488782929484767 21.696532986991848 68-69 21.97817773499939 27.77708996692415 28.571655195935854 21.6730771021406 70-71 22.034243164540722 27.884645891413413 28.54315502260209 21.537955921443775 72-73 22.17143980398209 27.57679581065166 28.566334509877628 21.68542987548862 74-75 21.924082866846366 28.47413238941569 28.101885444227104 21.499899299510844 76-77 22.03743874302938 27.904037355974992 28.313532229529564 21.744991671466064 78-79 21.985170336949064 27.98977326459867 28.29072595956694 21.734330438885323 80-81 21.9934783448489 28.33294932782941 28.048980706827663 21.62459162049403 82-83 22.11622071415637 28.214885012373998 28.078193310281197 21.59070096318844 84-85 21.86385702217376 28.229572809900905 27.99497200209592 21.911598165829417 86-87 22.137872426135583 28.202159455948987 27.77255836828843 21.887409749626997 88-89 22.074927397136456 28.12923689198336 27.722357266116664 22.07347844476352 90-91 21.99962256691071 28.07708881097192 27.954233305399605 21.969055316717768 92-93 22.10533723174826 28.120330215429277 27.900354472437666 21.873978080384802 94-95 21.86067577897546 27.997651552155688 27.891474665187506 22.25019800368135 96-97 21.889019419100308 27.867901125952454 27.849960409669354 22.39311904527788 98-99 21.823882944238903 28.001687879596084 27.505467839717685 22.668961336447328 100-101 21.920155961561687 28.042440867449926 27.476010558459706 22.56139261252868 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 860.0 1 619.0 2 521.5 3 655.5 4 519.5 5 457.5 6 809.0 7 1562.0 8 2365.5 9 2830.5 10 2567.0 11 2079.5 12 2071.0 13 2276.5 14 2487.5 15 2724.5 16 3069.0 17 3364.0 18 3537.5 19 3851.0 20 4294.5 21 4814.5 22 5531.5 23 6397.0 24 7376.0 25 8835.5 26 11185.5 27 13606.5 28 15477.0 29 17819.0 30 20756.5 31 24219.5 32 28345.0 33 31373.0 34 34140.0 35 36519.0 36 39174.0 37 42452.5 38 43148.0 39 42397.5 40 42239.5 41 43456.5 42 44751.5 43 45926.0 44 47355.0 45 49004.0 46 49611.0 47 52232.5 48 61730.0 49 61512.5 50 52161.0 51 46926.0 52 42600.0 53 40734.5 54 39992.5 55 37316.5 56 33651.5 57 29873.5 58 27118.0 59 24489.5 60 20869.5 61 17326.0 62 14316.0 63 11146.5 64 8570.5 65 6917.5 66 5154.0 67 3915.5 68 2815.0 69 1975.5 70 1542.5 71 1234.0 72 1005.0 73 866.5 74 734.0 75 595.0 76 460.5 77 312.5 78 231.5 79 181.0 80 121.5 81 78.5 82 65.5 83 41.5 84 22.5 85 12.5 86 6.5 87 4.0 88 3.0 89 1.0 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017587807993555277 2 0.010207825815867376 3 0.006759236013209479 4 0.0 5 0.002482984657913686 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 7.586897565847374E-4 14-15 3.793448782923687E-4 16-17 6.897179605315794E-5 18-19 0.0010690628388239482 20-21 0.0 22-23 3.448589802657897E-5 24-25 0.0 26-27 0.00134495002303658 28-29 0.0026554141480465807 30-31 0.0014139218190897377 32-33 0.008242129628352374 34-35 0.0018277525954086854 36-37 8.966333486910533E-4 38-39 0.0010345769407973692 40-41 4.4831667434552667E-4 42-43 0.005655687276358951 44-45 0.0018967243914618436 46-47 2.069153881594738E-4 48-49 7.931756546113163E-4 50-51 3.448589802657897E-5 52-53 2.414012861860528E-4 54-55 0.0018622384934352644 56-57 0.015656597704066855 58-59 0.025554050437695014 60-61 0.02103639779621317 62-63 0.014553048967216325 64-65 0.019243131098831065 66-67 0.0032071885164718446 68-69 0.001275978226983422 70-71 1.3794359210631587E-4 72-73 3.448589802657897E-5 74-75 0.0016553231052757905 76-77 0.002276069269754212 78-79 0.009552593753362376 80-81 0.016277343868545274 82-83 0.017622293891581854 84-85 0.026519655582439232 86-87 0.01041474120402685 88-89 0.037589628848971074 90-91 0.041693450714133974 92-93 0.026899000460731598 94-95 0.027588718421263175 96-97 0.04486615333257924 98-99 0.04914240468787503 100-101 0.054694634270154245 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1449868.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.836991046788874 #Duplication Level Percentage of deduplicated Percentage of total 1 78.71406212344884 43.164423199199476 2 12.300323782780433 13.490254902978698 3 3.8857928867772222 6.392555692256353 4 1.6571965364509702 3.6350268652852553 5 0.8634084744638054 2.367336139194667 6 0.534340443073997 1.7580973255671581 7 0.35598094985164846 1.366464691188957 8 0.24728801927831429 1.084842471931446 9 0.17543623600235364 0.8658355772649066 >10 1.0961573867097172 11.578373982026445 >50 0.10733405083090462 4.05468171161444 >100 0.05661345195019669 5.61331950730095 >500 0.004422875903234169 1.6588851947932401 >1k 0.0011373109465459292 1.0124888918135273 >5k 5.054715317981907E-4 1.957413847584471 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9225 0.6362648185903821 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8605 0.5935023050374241 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5326 0.3673437857791192 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5091 0.3511354137066271 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2448 0.16884295673813063 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2379 0.16408390281046273 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1897 0.13083949711284062 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1525 0.10518198898106586 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1499 0.10338872228368376 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 6.897179605315794E-5 0.0 3 0.0 0.0 0.0 6.897179605315794E-5 0.0 4 0.0 0.0 0.0 6.897179605315794E-5 0.0 5 0.0 0.0 0.0 6.897179605315794E-5 0.0 6 0.0 0.0 0.0 6.897179605315794E-5 0.0 7 0.0 0.0 0.0 6.897179605315794E-5 0.0 8 0.0 0.0 0.0 6.897179605315794E-5 0.0 9 0.0 0.0 0.0 6.897179605315794E-5 0.0 10-11 0.0 0.0 0.0 6.897179605315794E-5 0.0 12-13 0.0 0.0 0.0 6.897179605315794E-5 0.0 14-15 0.0 0.0 0.0 6.897179605315794E-5 0.0 16-17 0.0 0.0 0.0 6.897179605315794E-5 0.0 18-19 0.0 0.0 0.0 6.897179605315794E-5 0.0 20-21 0.0 0.0 0.0 6.897179605315794E-5 6.897179605315794E-5 22-23 0.0 0.0 0.0 2.0691538815947384E-4 6.897179605315794E-5 24-25 0.0 0.0 0.0 2.7588718421263174E-4 6.897179605315794E-5 26-27 0.0 0.0 0.0 3.7934487829236873E-4 6.897179605315794E-5 28-29 0.0 0.0 0.0 7.586897565847374E-4 6.897179605315794E-5 30-31 0.0 0.0 0.0 0.002034667983568159 6.897179605315794E-5 32-33 0.0 0.0 0.0 0.004448680845428687 6.897179605315794E-5 34-35 0.0 0.0 0.0 0.008000728342166322 6.897179605315794E-5 36-37 0.0 0.0 0.0 0.013690901516551852 6.897179605315794E-5 38-39 0.0 0.0 0.0 0.023933213230445807 6.897179605315794E-5 40-41 0.0 0.0 0.0 0.034485898026578976 6.897179605315794E-5 42-43 0.0 0.0 0.0 0.044728209740472924 6.897179605315794E-5 44-45 0.0 0.0 0.0 0.0590398574215032 6.897179605315794E-5 46-47 0.0 0.0 0.0 0.07714495388545715 6.897179605315794E-5 48-49 0.0 0.0 0.0 0.09631911318823506 6.897179605315794E-5 50-51 0.0 0.0 0.0 0.11890737639564429 6.897179605315794E-5 52-53 0.0 0.0 0.0 0.14273713193201037 6.897179605315794E-5 54-55 0.0 0.0 0.0 0.1704293080473533 6.897179605315794E-5 56-57 0.0 0.0 0.0 0.20626015609696885 6.897179605315794E-5 58-59 0.0 0.0 0.0 0.24929855683413937 6.897179605315794E-5 60-61 0.0 0.0 0.0 0.29726844098911076 6.897179605315794E-5 62-63 0.0 0.0 0.0 0.3468246764533047 6.897179605315794E-5 64-65 0.0 0.0 0.0 0.4040712671774258 6.897179605315794E-5 66-67 0.0 0.0 0.0 0.47090493755293583 6.897179605315794E-5 68-69 0.0 0.0 0.0 0.5486016658068182 6.897179605315794E-5 70-71 0.0 0.0 0.0 0.6379201416956578 6.897179605315794E-5 72-73 0.0 0.0 0.0 0.7317907561240058 6.897179605315794E-5 74-75 0.0 0.0 0.0 0.8335586412004403 6.897179605315794E-5 76-77 0.0 0.0 0.0 0.9453274367045827 6.897179605315794E-5 78-79 0.0 0.0 0.0 1.07620141971545 6.897179605315794E-5 80-81 0.0 0.0 0.0 1.2209732196310286 6.897179605315794E-5 82-83 0.0 0.0 0.0 1.3660553926288461 6.897179605315794E-5 84-85 0.0 0.0 0.0 1.5245180940609766 6.897179605315794E-5 86-87 0.0 0.0 0.0 1.6899814327925027 6.897179605315794E-5 88-89 0.0 0.0 0.0 1.8783089219156501 6.897179605315794E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATATGCG 110 9.4916926E-5 25.903713 5 TAAGGTG 865 0.0 24.156834 5 GGTAAGG 700 0.0 23.746706 3 GTAAGGT 720 0.0 23.745068 4 GAGTACT 9745 0.0 22.320404 12-13 GTACTTT 10240 0.0 21.194328 14-15 AGTACTT 9935 0.0 21.128702 12-13 ACCGTCG 115 0.0037274782 20.648598 8 GCACCGT 300 1.6734703E-10 20.57906 6 GGTATCA 14710 0.0 20.412096 1 AGGTAAG 775 0.0 20.225094 2 GTATCAA 18430 0.0 19.92679 1 CGATCGT 195 0.0 19.48515 24-25 GTACATG 8505 0.0 18.992746 1 CCGACGG 205 1.5922347E-5 18.533375 9 ACTTTTT 11910 0.0 18.421867 16-17 TACTTTT 11440 0.0 18.161657 14-15 TACATGG 8700 0.0 18.125797 2 AAGGTGT 1125 0.0 17.72965 6 ACATGGG 8605 0.0 17.385736 3 >>END_MODULE