FastQCFastQC Report
Thu 26 May 2016
SRR936419_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936419_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1487246
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT125560.8442450004908401No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117690.7913284016228653No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89150.5994300875578081No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT79400.5338726747289958No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57130.3841328199907749No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT54510.3665163664921607No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC48930.32899735484244036No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT37430.2516732268905077No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT33630.2261226454803039No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC30290.20366502918817736No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC29460.1980842443012118No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC26260.17656796521893486No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG23720.15948941869737757No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA23020.1547827326481295No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20450.13750247101017585No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA19430.13064415705270008No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17920.12049116286075066No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17070.11477590122952086No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA15350.10321090122279704No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15230.10240404075721166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG118050.024.91191
TACATGG118150.024.409922
GAGTACT88400.023.53947312-13
AGTACTT90950.022.95784212-13
ACATGGG120200.022.9263523
GTACTTT97100.021.48147214-15
GTGTAGG5400.021.115421
TACCTGG10400.021.0153142
GTACCTG10900.020.9216981
CATGGGG39100.020.900814
GTACACG2056.8186273E-720.8579121
ATGGGAT29950.020.6239835
CATGGGA77900.020.0054264
TACTTTT103750.019.944314-15
ACTTTTT108850.019.5121516-17
AGAGTAC170100.019.17554310-11
ATGGGAG27700.019.04015
GTATCAA273950.018.140221
CTGTACG1600.001189072617.8173334
ATGGGGG20400.017.7014755
Produced by FastQC (version 0.11.5)