##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936419_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1487246 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.75808171613842 31.0 31.0 34.0 30.0 34.0 2 31.823412535653148 33.0 31.0 34.0 30.0 34.0 3 31.83270218914692 33.0 31.0 34.0 30.0 34.0 4 35.48084983923305 37.0 35.0 37.0 33.0 37.0 5 35.51189984709994 37.0 35.0 37.0 33.0 37.0 6 35.54359130903697 37.0 35.0 37.0 33.0 37.0 7 35.48439397382813 37.0 35.0 37.0 33.0 37.0 8 35.45470419822948 37.0 35.0 37.0 33.0 37.0 9 37.00078937848883 39.0 37.0 39.0 33.0 39.0 10-11 36.88441253161884 39.0 37.0 39.0 33.0 39.0 12-13 36.788876554383066 39.0 37.0 39.0 33.0 39.0 14-15 37.86832810442927 40.0 37.5 41.0 33.0 41.0 16-17 37.80853470105147 40.0 37.0 41.0 33.0 41.0 18-19 37.834197906735 40.0 37.5 41.0 33.0 41.0 20-21 37.72138771931476 40.0 37.0 41.0 32.5 41.0 22-23 37.818780148005104 40.0 37.0 41.0 33.0 41.0 24-25 37.83134397402985 40.0 37.0 41.0 33.0 41.0 26-27 37.72444269475258 40.0 37.0 41.0 33.0 41.0 28-29 37.593872499909224 40.0 37.0 41.0 32.5 41.0 30-31 37.351153070843694 39.5 36.5 41.0 32.0 41.0 32-33 37.093860397002246 39.0 36.0 41.0 31.5 41.0 34-35 36.85248506299563 39.0 35.0 41.0 31.0 41.0 36-37 36.62013345472101 39.0 35.0 41.0 30.5 41.0 38-39 36.39540835880547 39.0 35.0 40.0 30.0 41.0 40-41 36.1847834857179 39.0 35.0 40.0 30.0 41.0 42-43 35.89466873671202 38.0 35.0 40.0 29.5 41.0 44-45 35.539904292901106 38.0 35.0 40.0 28.0 41.0 46-47 35.17311897292042 38.0 35.0 40.0 26.5 41.0 48-49 34.89974321665683 38.0 34.5 40.0 24.5 41.0 50-51 33.891421795721755 37.5 33.0 39.5 23.5 40.5 52-53 33.98668680231784 37.5 33.5 39.5 23.5 40.5 54-55 34.40840755328977 38.0 34.0 40.0 23.5 41.0 56-57 34.265243611346065 38.0 34.0 40.0 23.0 41.0 58-59 34.01986591323829 38.0 34.0 40.0 22.5 41.0 60-61 33.68900067641802 37.0 33.0 40.0 22.0 41.0 62-63 33.353410262996164 37.0 33.0 40.0 20.5 41.0 64-65 32.999865187063875 36.0 33.0 39.0 19.5 41.0 66-67 32.512147620501246 36.0 32.0 39.0 18.0 41.0 68-69 32.11042322520955 35.0 32.0 39.0 14.5 40.0 70-71 31.678303051411802 35.0 31.0 38.0 12.0 40.0 72-73 31.28994631688369 35.0 31.0 37.0 8.0 39.5 74-75 30.880707361122504 35.0 31.0 37.0 7.0 39.0 76-77 30.434898799526103 35.0 30.0 36.0 4.0 39.0 78-79 29.950131652732637 34.0 30.0 36.0 2.0 37.5 80-81 29.477488256818308 34.0 29.0 35.0 2.0 37.0 82-83 29.063864014426663 34.0 29.0 35.0 2.0 37.0 84-85 28.70007887061051 34.0 29.0 35.0 2.0 36.0 86-87 28.391304800954245 34.0 29.0 35.0 2.0 36.0 88-89 27.97296143341451 34.0 27.5 35.0 2.0 35.5 90-91 27.54267316906551 34.0 26.5 35.0 2.0 35.0 92-93 27.091125140023912 33.0 25.5 35.0 2.0 35.0 94-95 26.597862424911547 33.0 25.0 35.0 2.0 35.0 96-97 26.014673429950392 33.0 24.0 35.0 2.0 35.0 98-99 25.277474607428765 33.0 19.0 35.0 2.0 35.0 100-101 22.368712035534138 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3311420473167104 1101 2 0.30935002252292776 1101 3 0.4024701952780063 1101 4 0.29134796884586933 1101 5 0.16911376787409438 1101 6 0.32002367639421436 1101 7 0.4137227462484958 1101 8 0.2888492888081444 1101 9 0.4133575467123407 1101 10-11 0.5735237499929227 1101 12-13 0.6002943551162474 1101 14-15 0.6143629413730878 1101 16-17 0.6863898566862616 1101 18-19 0.744498368121647 1101 20-21 0.7592153033181503 1101 22-23 0.6091101704398056 1101 24-25 0.5767437574113217 1101 26-27 0.5306021228618789 1101 28-29 0.5957906985845582 1101 30-31 0.6239872992949032 1101 32-33 0.5042702732886539 1101 34-35 0.5371599911369671 1101 36-37 0.48153762881344164 1101 38-39 0.5132284890329757 1101 40-41 0.5578489354542597 1101 42-43 0.6216938949704982 1101 44-45 0.5771900482775862 1101 46-47 0.43576693638079433 1101 48-49 0.5841970899289421 1101 50-51 0.4083382669008131 1101 52-53 0.5507069850247319 1101 54-55 0.445167449384094 1101 56-57 0.49671495103121543 1101 58-59 0.5651741579447318 1101 60-61 0.6463887105009292 1101 62-63 0.6609384728571115 1101 64-65 0.567814421551688 1101 66-67 0.7846792931389857 1101 68-69 0.6617409354217827 1101 70-71 0.5333041949712403 1101 72-73 0.3970321070448719 1101 74-75 0.39218541647442606 1101 76-77 0.3391236320731217 1101 78-79 0.32264998967366054 1101 80-81 0.42046616689485106 1101 82-83 0.27903985470661397 1101 84-85 0.39166423571408515 1101 86-87 0.3082957262434505 1101 88-89 0.2154498552788091 1101 90-91 0.3493867712283141 1101 92-93 0.23163097880775751 1101 94-95 0.4991050972758124 1101 96-97 0.44625068347607666 1101 98-99 0.5951800199407593 1101 100-101 0.7463638358947691 1102 1 0.17857725700082128 1102 2 0.26117262421273324 1102 3 0.17649841887588025 1102 4 0.22889739899691364 1102 5 0.10991916442454652 1102 6 0.277582055674344 1102 7 0.23049465500749022 1102 8 0.3203699012594612 1102 9 0.24356944303780637 1102 10-11 0.4163568637759667 1102 12-13 0.3038499105325414 1102 14-15 0.4316465422820386 1102 16-17 0.24205259359063547 1102 18-19 0.20026441482507096 1102 20-21 0.2375255723811165 1102 22-23 0.14289830294206496 1102 24-25 0.04393858772255044 1102 26-27 -0.09770068859350545 1102 28-29 -0.2874042094361968 1102 30-31 -0.05046854602804984 1102 32-33 0.03257017063688039 1102 34-35 0.0945365677316019 1102 36-37 -0.4276512717597285 1102 38-39 -0.315559724938943 1102 40-41 -0.43488773078208 1102 42-43 -0.44133808625888804 1102 44-45 -0.39203415999396185 1102 46-47 -0.23525370928508949 1102 48-49 -0.15955105350278842 1102 50-51 -0.10181551648764753 1102 52-53 0.0032941481633415037 1102 54-55 0.06624922863138494 1102 56-57 -0.07228640637870143 1102 58-59 -0.2342094613888932 1102 60-61 -0.34373387627366014 1102 62-63 -0.27301266163927096 1102 64-65 -0.30955916573302744 1102 66-67 -0.14851323346086076 1102 68-69 -0.1931399747322189 1102 70-71 -0.3081431511858348 1102 72-73 -0.41422743900616865 1102 74-75 -0.34089552802217327 1102 76-77 -0.22165759305513077 1102 78-79 -0.2933469859217901 1102 80-81 -0.0657905277179971 1102 82-83 0.046966313922276015 1102 84-85 -0.3343949639028807 1102 86-87 -0.3089190641351074 1102 88-89 -0.5252892375000933 1102 90-91 -0.6166088883187406 1102 92-93 -0.6629218968833257 1102 94-95 -0.5453297100735028 1102 96-97 -0.5062622169316455 1102 98-99 -0.7154927878672943 1102 100-101 -0.484383159441407 1103 1 0.34587342953845024 1103 2 0.2687389546231458 1103 3 0.39651402773931466 1103 4 0.43952058883818523 1103 5 0.4421924414889702 1103 6 0.3987330335189867 1103 7 0.5336747270250655 1103 8 0.5201462537990977 1103 9 0.44848562202159314 1103 10-11 0.49613160961572333 1103 12-13 0.28965336209812165 1103 14-15 0.5336497227734753 1103 16-17 0.631937191888241 1103 18-19 0.6576489590820742 1103 20-21 0.747859104562373 1103 22-23 0.44842825418291454 1103 24-25 0.5261053483284641 1103 26-27 0.5296852849165887 1103 28-29 0.5876626154054563 1103 30-31 0.5059212961443436 1103 32-33 0.43334370951594536 1103 34-35 0.591557075093867 1103 36-37 0.7117347817627433 1103 38-39 0.8246558115907376 1103 40-41 0.6782852401382655 1103 42-43 0.8300841754389623 1103 44-45 0.9473279543456385 1103 46-47 1.1644214340677124 1103 48-49 1.0597628340301597 1103 50-51 1.0358330104944287 1103 52-53 1.149214598452275 1103 54-55 1.168474217589356 1103 56-57 1.0498886006627046 1103 58-59 0.9658570972825942 1103 60-61 1.060347109143109 1103 62-63 1.1988844476237404 1103 64-65 1.0263417686371525 1103 66-67 0.9860791761404926 1103 68-69 0.8678894552990641 1103 70-71 1.0302843026046027 1103 72-73 0.8869537757686281 1103 74-75 0.9760924839964567 1103 76-77 0.9964362672542535 1103 78-79 0.8289001290356488 1103 80-81 0.8411550938796601 1103 82-83 0.6435770482905845 1103 84-85 0.8248278643986531 1103 86-87 0.9766981328446818 1103 88-89 0.767607310063223 1103 90-91 0.8846943775729876 1103 92-93 0.9974362681363615 1103 94-95 1.0873733753387285 1103 96-97 1.1784785865666976 1103 98-99 1.1535584300466972 1103 100-101 0.9720313962270346 1104 1 0.27875627631959077 1104 2 0.31047090677606093 1104 3 0.22516550676447622 1104 4 0.3796189010998319 1104 5 0.309016728357733 1104 6 0.32333313904437944 1104 7 0.359913815499425 1104 8 0.482020197710348 1104 9 0.4269768093158035 1104 10-11 0.5724009197601916 1104 12-13 0.5639226989578745 1104 14-15 0.35009047828184237 1104 16-17 0.3461113221386327 1104 18-19 0.4017276823584339 1104 20-21 0.07916224294089602 1104 22-23 0.11292947974677503 1104 24-25 0.2635675294981965 1104 26-27 0.2999114298336707 1104 28-29 0.2977781924222924 1104 30-31 0.304045548951116 1104 32-33 0.21265355343187053 1104 34-35 0.22544324792843184 1104 36-37 -0.0328029435640218 1104 38-39 -0.050193580970393725 1104 40-41 0.03019137597215149 1104 42-43 0.1367882101154123 1104 44-45 0.03531191849371851 1104 46-47 0.08519741301606132 1104 48-49 0.36268882600221275 1104 50-51 0.36281077669558215 1104 52-53 0.36224080615028953 1104 54-55 0.3549933490753361 1104 56-57 0.4226996167119168 1104 58-59 0.29569490208734095 1104 60-61 0.4465917410398319 1104 62-63 0.5271685914321367 1104 64-65 0.30776278822613534 1104 66-67 0.3885388193578123 1104 68-69 0.5048839519496369 1104 70-71 0.49807418018179206 1104 72-73 0.2630053563118864 1104 74-75 0.43487112779483894 1104 76-77 0.20118157037259365 1104 78-79 0.3510217236712201 1104 80-81 0.5512716727198175 1104 82-83 0.49520835059411183 1104 84-85 0.24283831007645418 1104 86-87 0.4127611007796581 1104 88-89 0.39748067959577327 1104 90-91 0.37611609843829896 1104 92-93 0.04549089422250674 1104 94-95 0.19386068223727904 1104 96-97 0.2847311328676341 1104 98-99 0.43954576032113124 1104 100-101 0.4920418151895447 1105 1 0.4113468835681715 1105 2 0.33250866979710025 1105 3 0.3579427956142496 1105 4 0.3231850530586584 1105 5 0.2730271825274002 1105 6 0.46221491625322386 1105 7 0.532666302365044 1105 8 0.6207379617981417 1105 9 0.5668066623707091 1105 10-11 0.4997531079352413 1105 12-13 0.48345559492288004 1105 14-15 0.4542694792685893 1105 16-17 0.3854171332701952 1105 18-19 0.2944511002318606 1105 20-21 0.42499924323424665 1105 22-23 0.23960228023747732 1105 24-25 0.2976602569163802 1105 26-27 0.16395321984354183 1105 28-29 0.20030772811847442 1105 30-31 0.20502096031735562 1105 32-33 0.08743753615049599 1105 34-35 0.23563406319365043 1105 36-37 0.3484582239960474 1105 38-39 0.18577396309979832 1105 40-41 0.20855297823366215 1105 42-43 0.21067078738825984 1105 44-45 0.2836564527231289 1105 46-47 0.5352527507764222 1105 48-49 0.453897456025949 1105 50-51 0.42163481595204644 1105 52-53 0.5465615518099369 1105 54-55 0.3695558474085274 1105 56-57 0.2368947523227618 1105 58-59 0.31613653741816705 1105 60-61 0.30316360012189136 1105 62-63 0.2799121442496926 1105 64-65 0.19209820172588365 1105 66-67 0.3331557220433652 1105 68-69 0.3687323931582682 1105 70-71 0.34916826770879794 1105 72-73 0.19717142293054835 1105 74-75 0.249441626768089 1105 76-77 0.33606169411583053 1105 78-79 0.41389141991358613 1105 80-81 0.6012390034396091 1105 82-83 0.4346081692462711 1105 84-85 0.30527463831604607 1105 86-87 0.18135525497866212 1105 88-89 0.179545244760984 1105 90-91 0.12926701717846 1105 92-93 0.014069713861410094 1105 94-95 0.11109006058755 1105 96-97 0.2555221294806955 1105 98-99 0.44395878002937295 1105 100-101 0.33190300408465845 1106 1 0.12184154387913182 1106 2 0.12537909415864235 1106 3 0.24204327174841467 1106 4 0.11084933157879107 1106 5 0.12076551125978341 1106 6 0.23817870395963325 1106 7 0.25527975291736027 1106 8 0.25420572182 1106 9 0.19056790480496488 1106 10-11 0.2632848055852932 1106 12-13 0.31599378555868185 1106 14-15 0.36140241897230396 1106 16-17 0.30983372280121557 1106 18-19 0.21118215986484046 1106 20-21 0.07339037052911834 1106 22-23 0.23074102652172002 1106 24-25 0.2199641115235238 1106 26-27 0.09965386968580248 1106 28-29 0.026970867613691496 1106 30-31 0.28126410136207625 1106 32-33 0.266538671505522 1106 34-35 0.10836195992568065 1106 36-37 0.09819113760277531 1106 38-39 0.13632390281846796 1106 40-41 0.19780444992726132 1106 42-43 0.3126386636232823 1106 44-45 0.12422218655828487 1106 46-47 -0.009447861007728875 1106 48-49 0.01771377398515739 1106 50-51 -0.15596788318362087 1106 52-53 -0.13293069860700513 1106 54-55 0.131409929888612 1106 56-57 0.2817611887378533 1106 58-59 0.17629557275376584 1106 60-61 0.22044888101115134 1106 62-63 0.2554779868223136 1106 64-65 0.13428996839703444 1106 66-67 0.30671196037956605 1106 68-69 0.3777810490952618 1106 70-71 0.195860677969808 1106 72-73 0.03069266819197125 1106 74-75 0.2007449132219854 1106 76-77 0.13474923293849983 1106 78-79 0.15122979186910612 1106 80-81 0.46791056329210434 1106 82-83 0.229531522927104 1106 84-85 0.2581293025415583 1106 86-87 0.1472499196391226 1106 88-89 0.17093986388763582 1106 90-91 0.1380618391004802 1106 92-93 0.26233280279370774 1106 94-95 0.1661810466596414 1106 96-97 0.024597908618599007 1106 98-99 -0.22461045243365163 1106 100-101 -0.17693582252938 1107 1 0.2331482697476268 1107 2 0.113596595529593 1107 3 0.13968660182414894 1107 4 0.18792268064527207 1107 5 0.18129761290094137 1107 6 0.30111536937023686 1107 7 0.3785323679315198 1107 8 0.42659599232321455 1107 9 0.4542961972685404 1107 10-11 0.394737071556456 1107 12-13 0.35269810352752273 1107 14-15 0.2012848186479701 1107 16-17 0.267614123904508 1107 18-19 0.2497465767462188 1107 20-21 0.14472139392902506 1107 22-23 0.19014818445601378 1107 24-25 0.13331046163467875 1107 26-27 0.08895315243547941 1107 28-29 0.15128841435954854 1107 30-31 0.2008660956794941 1107 32-33 0.0010369111429113786 1107 34-35 0.22490977701135506 1107 36-37 0.22858544997889396 1107 38-39 0.12070462042280639 1107 40-41 0.22476984211385798 1107 42-43 0.21561584307406179 1107 44-45 0.21751970332879011 1107 46-47 0.4322889529520779 1107 48-49 0.5478511547739089 1107 50-51 0.7281630511684511 1107 52-53 0.8281803160583152 1107 54-55 0.78613836634009 1107 56-57 0.57342143041285 1107 58-59 0.49839631866550604 1107 60-61 0.6733627976962779 1107 62-63 0.695688631237914 1107 64-65 0.7517920882187923 1107 66-67 0.7092052656233463 1107 68-69 0.8062973576814016 1107 70-71 0.5317369464163413 1107 72-73 0.3735894527006458 1107 74-75 0.5228676147343982 1107 76-77 0.5548325484860932 1107 78-79 0.4239030343232706 1107 80-81 0.5038512007942479 1107 82-83 0.7975572923347478 1107 84-85 0.5401096772981298 1107 86-87 0.5987202130306208 1107 88-89 0.45761312063847015 1107 90-91 0.4654613735055868 1107 92-93 0.6569365586651266 1107 94-95 0.9296162573840476 1107 96-97 0.7833594697741368 1107 98-99 0.8313620326033515 1107 100-101 0.8090447605501012 1108 1 0.14137771650638697 1108 2 0.15952648781262724 1108 3 0.12571311687719344 1108 4 0.2002390626031172 1108 5 0.025010001370567636 1108 6 0.10254897248195505 1108 7 0.09120820458058176 1108 8 0.08581594191805664 1108 9 0.13691231919084856 1108 10-11 0.23246534039147093 1108 12-13 -0.11486262119945678 1108 14-15 0.10197966367704225 1108 16-17 0.06926869750174092 1108 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8678.0 15 11214.0 16 15063.0 17 21327.0 18 15629.0 19 10020.0 20 9492.0 21 9874.0 22 10617.0 23 11643.0 24 13234.0 25 15517.0 26 18234.0 27 21363.0 28 25454.0 29 31341.0 30 38797.0 31 48658.0 32 63124.0 33 82651.0 34 112598.0 35 157780.0 36 213868.0 37 267714.0 38 194700.0 39 26811.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.37148265578191 18.293922042640183 14.344342493250265 20.990252808327643 2 15.506428705920328 21.018707803211665 39.65314576216478 23.82171772870323 3 20.488596413364213 26.293881415610233 29.562241607019413 23.65528056400614 4 12.293762331892848 16.37170998212506 38.063467469357725 33.27106021662436 5 12.866704958629882 38.87970449724311 32.99009565368282 15.263494890444182 6 30.064555794651092 36.316115539871966 17.6369767412138 15.982351924263144 7 27.8228786257843 34.31222308815253 20.375534323452538 17.48936396261064 8 27.072062498823037 31.792967268285988 19.751949725464666 21.38302050742631 9 27.438316922851342 16.892449519187114 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21.97615220577136 34-35 22.566348326486786 25.498177671406637 30.486594176953304 21.448879825153277 36-37 22.250462433459003 25.079257497607987 31.079361045049186 21.590919023883824 38-39 21.80340159153334 25.797296343238674 30.86068536118797 21.53861670404002 40-41 21.727065386073956 26.132038934178215 30.527027463173095 21.613868216574726 42-43 21.356182473957844 26.270000618610496 29.62905559694568 22.744761310485988 44-45 21.856056416347588 25.71662659772859 29.6641706368591 22.76314634906472 46-47 22.75330185428304 26.418599647331536 29.174422963457292 21.65367553492813 48-49 22.447232758151177 26.486328021252803 28.73048649208365 22.335952728512364 50-51 21.65799429731619 26.975859579900273 29.37705861607207 21.989087506711464 52-53 22.500702663666843 27.35530171503727 27.94711934792812 22.196876273367767 54-55 21.571094285829556 26.740239917158647 28.59159617531166 23.097069621700133 56-57 22.523474770295213 26.772735983217178 27.970697300695583 22.733091945792022 58-59 23.34552538157166 26.244215949539107 27.89173945304896 22.51851921584027 60-61 22.332866013764075 26.61015125688792 29.316671205381905 21.740311523966096 62-63 22.012377322765182 26.853875242395965 29.00151690787474 22.132230526964115 64-65 21.67134200624514 27.623860638123336 28.488518298373627 22.216279057257893 66-67 22.124921277356226 26.97104183555819 27.76073965156593 23.143297235519658 68-69 21.871335116524588 26.972836098086955 28.3642768473367 22.79155193805176 70-71 22.571980376051677 26.890264780690337 27.970853337692418 22.566901505565568 72-73 22.31709957728843 26.528277830304773 28.713486825736044 22.441135766670747 74-75 21.655907874789992 28.161356320694946 27.815254572430497 22.36748123208456 76-77 22.008643579458788 26.95755015186121 27.91835139795707 23.115454870722935 78-79 22.109234358476197 27.139466609978392 28.10136213312954 22.649936898415866 80-81 22.609951918272838 27.304741791603682 27.593010099147747 22.492296190975736 82-83 22.243318447881354 26.992491799099206 28.32464837786468 22.43954137515476 84-85 21.93517409223252 27.45476083438382 27.92466241900292 22.68540265438074 86-87 22.01489940130029 27.698393884217765 27.548256608646092 22.73845010583585 88-89 21.82813010688908 27.63708823762813 26.985154966972928 23.54962668850986 90-91 22.04277117732897 26.966679457640296 27.490279812917716 23.500269552113014 92-93 22.65364234981112 27.328535467153042 27.611403776016186 22.406418407019654 94-95 22.135923113546315 27.03305009293405 27.756449569490734 23.0745772240289 96-97 21.34410091769032 27.098058230228233 28.4012242147581 23.156616637323353 98-99 21.78650556519218 27.633228423633373 27.109419908894385 23.470846102280063 100-101 21.780390513007962 26.902463255046595 27.49016047037308 23.82698576157236 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 733.0 1 621.5 2 995.5 3 1302.0 4 904.5 5 798.5 6 1329.0 7 2549.5 8 4044.5 9 4612.0 10 3725.5 11 2784.0 12 2640.5 13 2710.0 14 2910.0 15 3071.0 16 3109.0 17 3215.5 18 3331.0 19 3436.0 20 3652.5 21 4097.0 22 4681.5 23 5299.5 24 6009.0 25 7045.0 26 8399.5 27 10082.5 28 11743.5 29 13787.0 30 16604.5 31 19560.0 32 23203.5 33 25980.0 34 27702.0 35 29311.0 36 32140.0 37 35312.5 38 36347.5 39 36801.0 40 37519.5 41 39336.0 42 40717.5 43 42612.0 44 45152.5 45 47468.0 46 49763.5 47 59143.5 48 80828.0 49 79909.0 50 59268.0 51 50071.5 52 46503.0 53 45957.5 54 45685.5 55 42640.5 56 38812.0 57 35603.0 58 33635.5 59 30662.5 60 25950.0 61 22249.5 62 19350.5 63 14949.5 64 10695.5 65 8369.5 66 6338.5 67 4904.0 68 3718.0 69 2686.0 70 2125.5 71 1708.5 72 1470.0 73 1345.5 74 1105.0 75 869.0 76 636.5 77 414.0 78 264.0 79 174.0 80 124.0 81 92.0 82 60.5 83 37.0 84 29.0 85 20.0 86 11.5 87 10.5 88 7.5 89 7.5 90 8.5 91 3.5 92 1.0 93 2.0 94 1.5 95 1.0 96 1.0 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010152994191949415 2 0.011161569773931146 3 0.011430523262459608 4 0.016675116288764603 5 0.0285090697840169 6 0.04202398258257208 7 0.005715261631229804 8 0.02487819768888267 9 0.03314851746113286 10-11 0.039099113394825066 12-13 0.027870305248761804 14-15 0.026592776178251613 16-17 0.025449723852005654 18-19 0.02033960756996489 20-21 0.0493193459589066 22-23 0.010724520355072396 24-25 0.00245420058282221 26-27 0.00100857558198173 28-29 6.723837213211533E-5 30-31 0.00201715116396346 32-33 0.001512863372972595 34-35 3.361918606605767E-5 36-37 3.3619186066057667E-4 38-39 6.723837213211533E-5 40-41 0.001210290698378076 42-43 0.002824011629548844 44-45 0.0011766715123120183 46-47 9.07718023783557E-4 48-49 4.706686049248073E-4 50-51 0.0034627761648039395 52-53 0.003227441862341536 54-55 0.004437732560719612 56-57 7.396220934532687E-4 58-59 0.005009258723842593 60-61 0.0027567732574167287 62-63 6.723837213211533E-4 64-65 6.723837213211533E-4 66-67 0.014893299427263548 68-69 0.03936806688335352 70-71 0.046730668631820155 72-73 0.01210290698378076 74-75 0.026895348852846133 76-77 0.02434029071182575 78-79 0.05069773258761496 80-81 0.07601297969535638 82-83 0.04515056688671545 84-85 0.015632921520716814 86-87 0.018389694778133545 88-89 0.019095697685520756 90-91 0.034997572694766034 92-93 0.022524854664258635 94-95 0.011430523262459608 96-97 0.016944069777293062 98-99 0.04905039247037813 100-101 0.06868399713295581 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1487246.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.24828527531253 #Duplication Level Percentage of deduplicated Percentage of total 1 76.74590528632915 39.33096047825912 2 12.986137586570724 13.3103456732207 3 4.276158670444543 6.5743739827632925 4 1.9571315203178479 4.011985378982208 5 1.0324567086277383 2.6455817969082283 6 0.6619854596273602 2.0355371809855107 7 0.40609254197263794 1.4568082507433404 8 0.3086616033099794 1.265470231997214 9 0.22172890724015443 1.0226903662824072 >10 1.2449203505851871 11.918061500710001 >50 0.10115180090095276 3.604912663585768 >100 0.050050418030624426 4.802854318034401 >500 0.003678208434612645 1.2486902787324263 >1k 0.0031527500868108386 3.247819986892843 >5k 5.254583478018065E-4 1.886297871458047 >10k+ 2.6272917390090324E-4 1.6376100404445908 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12556 0.8442450004908401 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11769 0.7913284016228653 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8915 0.5994300875578081 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7940 0.5338726747289958 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5713 0.3841328199907749 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5451 0.3665163664921607 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4893 0.32899735484244036 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3743 0.2516732268905077 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3363 0.2261226454803039 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3029 0.20366502918817736 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2946 0.1980842443012118 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2626 0.17656796521893486 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2372 0.15948941869737757 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2302 0.1547827326481295 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2045 0.13750247101017585 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1943 0.13064415705270008 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1792 0.12049116286075066 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1707 0.11477590122952086 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1535 0.10321090122279704 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1523 0.10240404075721166 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 6.723837213211533E-5 0.0 22-23 0.0 0.0 0.0 2.6895348852846133E-4 0.0 24-25 0.0 0.0 0.0 3.3619186066057667E-4 0.0 26-27 0.0 0.0 0.0 7.06002907387211E-4 0.0 28-29 0.0 0.0 0.0 0.0019499127918313447 0.0 30-31 0.0 0.0 0.0 0.004807543607446246 0.0 32-33 0.0 0.0 0.0 0.009547848842760377 0.0 34-35 0.0 0.0 0.0 0.01499415698546172 0.0 36-37 0.0 0.0 0.0 0.01983531977897402 0.0 38-39 0.0 0.0 0.0 0.027500494202035174 0.0 40-41 0.0 0.0 0.0 0.03960340118581593 0.0 42-43 0.0 0.0 0.0 0.053824316891758324 0.0 44-45 0.0 0.0 0.0 0.0714743895764386 0.0 46-47 0.0 0.0 0.0 0.09480610470628262 0.0 48-49 0.0 0.0 0.0 0.12129802332633606 0.0 50-51 0.0 0.0 0.0 0.15172338671611824 0.0 52-53 0.0 0.0 0.0 0.19428527627574727 0.0 54-55 0.0 0.0 0.0 0.23859536351081123 0.0 56-57 0.0 0.0 0.0 0.29386530540341005 0.0 58-59 0.0 0.0 0.0 0.3615743461404502 0.0 60-61 0.0 0.0 0.0 0.4425629653735831 0.0 62-63 0.0 0.0 0.0 0.5227111049550646 0.0 64-65 0.0 0.0 0.0 0.6055151602357647 0.0 66-67 0.0 0.0 0.0 0.7126595062282903 0.0 68-69 0.0 0.0 0.0 0.8419588958383482 0.0 70-71 0.0 0.0 0.0 0.9871601604576512 0.0 72-73 0.0 0.0 0.0 1.1197206111161166 0.0 74-75 0.0 0.0 0.0 1.27446972457818 0.0 76-77 0.0 0.0 0.0 1.430630843855018 0.0 78-79 0.0 0.0 0.0 1.6063583294223014 0.0 80-81 0.0 0.0 0.0 1.8119396522162439 0.0 82-83 0.0 0.0 0.0 2.042768983745796 0.0 84-85 0.0 0.0 0.0 2.2773636641147466 0.0 86-87 0.0 0.0 0.0 2.536433111939787 0.0 88-89 0.0 0.0 0.0 2.8394764551392306 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11805 0.0 24.9119 1 TACATGG 11815 0.0 24.40992 2 GAGTACT 8840 0.0 23.539473 12-13 AGTACTT 9095 0.0 22.957842 12-13 ACATGGG 12020 0.0 22.926352 3 GTACTTT 9710 0.0 21.481472 14-15 GTGTAGG 540 0.0 21.11542 1 TACCTGG 1040 0.0 21.015314 2 GTACCTG 1090 0.0 20.921698 1 CATGGGG 3910 0.0 20.90081 4 GTACACG 205 6.8186273E-7 20.857912 1 ATGGGAT 2995 0.0 20.623983 5 CATGGGA 7790 0.0 20.005426 4 TACTTTT 10375 0.0 19.9443 14-15 ACTTTTT 10885 0.0 19.51215 16-17 AGAGTAC 17010 0.0 19.175543 10-11 ATGGGAG 2770 0.0 19.0401 5 GTATCAA 27395 0.0 18.14022 1 CTGTACG 160 0.0011890726 17.817333 4 ATGGGGG 2040 0.0 17.701475 5 >>END_MODULE