FastQCFastQC Report
Thu 26 May 2016
SRR936419_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936419_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1487246
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT143750.9665515993991579No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111750.7513888085763888No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92890.6245772387352193No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT64580.43422540722920083No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63590.4275688083881214No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT47380.3185754071619625No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC42650.28677165714347186No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT37530.25234561061182886No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC36210.24347014549038962No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC34260.23035866292462712No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT30960.20817000012102907No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG27270.1833590408042785No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA26330.17703863382385968No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24110.16211171521053006No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC23320.15679988381209295No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA22310.1500088082267493No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21980.1477899419463895No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18580.12492889542147027No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17760.11941534890663683No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC17030.11450694774099242No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA15240.10247127912934376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTG6900.025.4717625
GTACATG107200.023.5790021
GTAAGGT6200.022.985324
TACATGG108850.022.6962592
GAGTACT102600.022.54544312-13
GGTAAGG6400.022.2685243
AGGTAAG6000.022.1710452
AGTACTT104850.022.15223712-13
ACATGGG108750.021.4050883
GTACTTT108000.021.3302114-15
TACCTGG10950.020.3921552
GTAAGCG2100.020.35568694-95
TACTTTT115550.019.8542914-15
CCTATAC3153.2014214E-1019.6057283
CATGGGA68950.019.221664
ACTTTTT121050.019.08920916-17
AGAGTAC180300.018.96766910-11
GTACCTG12800.018.5595991
ATGGGAG24750.018.0409345
GTGTAGC7650.018.0113051
Produced by FastQC (version 0.11.5)