##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936419_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1487246 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.627258032632128 31.0 31.0 34.0 30.0 34.0 2 31.73618755740476 31.0 31.0 34.0 30.0 34.0 3 31.832154868797765 31.0 31.0 34.0 30.0 34.0 4 35.63197009775114 37.0 35.0 37.0 33.0 37.0 5 35.491778764239406 37.0 35.0 37.0 33.0 37.0 6 35.56580552242198 37.0 35.0 37.0 33.0 37.0 7 35.48274797847834 37.0 35.0 37.0 33.0 37.0 8 35.454951635438924 37.0 35.0 37.0 33.0 37.0 9 36.97697421946336 39.0 37.0 39.0 33.0 39.0 10-11 36.857741758928924 39.0 37.0 39.0 33.0 39.0 12-13 36.71828500463272 39.0 35.0 39.0 32.0 39.0 14-15 37.629627176674205 39.0 36.5 41.0 32.0 41.0 16-17 37.631536074059035 39.0 36.5 40.5 32.5 41.0 18-19 37.84262959860037 39.5 37.0 41.0 33.0 41.0 20-21 37.864920799921464 40.0 37.0 41.0 33.0 41.0 22-23 37.74408772993842 39.5 37.0 41.0 32.0 41.0 24-25 37.58437844176417 39.0 36.0 41.0 32.0 41.0 26-27 37.31684200192839 39.0 36.0 41.0 31.5 41.0 28-29 37.290209218918726 39.0 36.0 41.0 32.0 41.0 30-31 37.10472914366554 39.0 36.0 40.5 31.0 41.0 32-33 36.822357901786255 39.0 35.0 40.0 31.0 41.0 34-35 36.56452832954333 38.5 35.0 40.0 30.0 41.0 36-37 36.31745588826596 38.0 35.0 40.0 30.0 41.0 38-39 36.05551200003228 38.0 35.0 40.0 30.0 41.0 40-41 35.79815813927219 38.0 35.0 40.0 29.5 41.0 42-43 35.44143974836712 38.0 34.5 40.0 27.5 41.0 44-45 35.0316874276347 38.0 34.0 40.0 26.5 41.0 46-47 34.49318337383325 38.0 33.5 40.0 24.5 41.0 48-49 33.95411821581635 38.0 33.0 40.0 23.0 41.0 50-51 33.56810171283029 37.5 33.0 40.0 21.5 41.0 52-53 33.26261358242012 37.0 32.5 40.0 20.0 41.0 54-55 32.894581999211965 37.0 32.0 40.0 18.5 41.0 56-57 32.48865587804573 36.0 31.0 39.0 16.5 40.0 58-59 32.07263794960618 36.0 31.0 39.0 13.5 40.0 60-61 31.59590477970692 35.0 30.0 39.0 10.5 40.0 62-63 31.238357675865323 35.0 30.0 38.0 8.0 40.0 64-65 30.886008434381402 35.0 29.5 38.0 7.5 40.0 66-67 30.352141811105895 34.0 29.0 37.5 6.5 40.0 68-69 29.84924787156933 34.0 28.0 37.0 2.0 39.0 70-71 29.276855342021427 34.0 27.5 36.0 2.0 39.0 72-73 28.65081869441908 33.0 26.5 36.0 2.0 38.5 74-75 28.07565896966608 33.0 26.0 35.0 2.0 38.0 76-77 26.854625260380597 31.5 25.0 34.0 2.0 36.0 78-79 27.60605172244538 32.5 26.0 35.0 2.0 36.0 80-81 27.3910573637448 33.0 26.0 35.0 2.0 36.0 82-83 26.916237125532696 32.0 25.0 35.0 2.0 36.0 84-85 26.39818261403964 32.0 24.5 34.5 2.0 35.5 86-87 25.930776751122544 32.0 23.5 34.0 2.0 35.0 88-89 25.309844168348747 31.0 20.5 34.0 2.0 35.0 90-91 24.586950309498228 31.0 18.5 34.0 2.0 35.0 92-93 23.87095544382032 31.0 12.0 34.0 2.0 35.0 94-95 22.96491266407844 30.0 2.0 34.0 2.0 35.0 96-97 21.938854096766775 29.0 2.0 34.0 2.0 35.0 98-99 20.639499114470638 29.0 2.0 34.0 2.0 35.0 100-101 16.960181772215222 22.0 2.0 31.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2972093287842199 1101 2 0.40660939672426366 1101 3 0.2572922817763903 1101 4 0.16781754148676242 1101 5 0.33530004283454673 1101 6 0.22984719307721235 1101 7 0.20276209867979844 1101 8 0.19664794623935933 1101 9 0.32922602249537647 1101 10-11 0.20523377110336583 1101 12-13 0.3434113563205017 1101 14-15 0.3399779440576367 1101 16-17 0.43136842451819035 1101 18-19 0.4211181670994719 1101 20-21 0.28469297791566817 1101 22-23 0.3138288673641725 1101 24-25 0.41676643030093175 1101 26-27 0.33081655200330573 1101 28-29 0.35044148354609916 1101 30-31 0.4653527514121478 1101 32-33 0.5020403994089051 1101 34-35 0.5317715228876878 1101 36-37 0.47525898567963765 1101 38-39 0.45634780446911805 1101 40-41 0.5137768416405564 1101 42-43 0.6321239589783616 1101 44-45 0.5589770643435088 1101 46-47 0.7068365227244016 1101 48-49 0.7847427518292065 1101 50-51 0.8602898002845123 1101 52-53 0.7696157207379812 1101 54-55 1.0030151063862718 1101 56-57 1.0494828287831126 1101 58-59 0.9453543836847196 1101 60-61 0.7312636080995212 1101 62-63 0.7112874245566339 1101 64-65 0.8075453768881822 1101 66-67 0.7568912450835477 1101 68-69 0.8103212011895877 1101 70-71 0.6303602255396861 1101 72-73 0.991339745493196 1101 74-75 1.0554918517264262 1101 76-77 1.056476851039708 1101 78-79 0.9702698179890525 1101 80-81 1.1428096640051955 1101 82-83 1.194663923637325 1101 84-85 1.0075020326817743 1101 86-87 0.9648086579383488 1101 88-89 0.9431143769431678 1101 90-91 0.8898151809585322 1101 92-93 1.0636580148152142 1101 94-95 0.9087871543807715 1101 96-97 0.9620534149352125 1101 98-99 0.3950043156443179 1101 100-101 0.43788941237548684 1102 1 0.29963649803932313 1102 2 0.40642044977605707 1102 3 0.28590254720454666 1102 4 0.30920814089040505 1102 5 0.12145157891370673 1102 6 0.1031170594544335 1102 7 0.20163311716338939 1102 8 0.18708936140500043 1102 9 0.2540834192513799 1102 10-11 0.3333095642105306 1102 12-13 0.40791182821785554 1102 14-15 0.39307893009122097 1102 16-17 0.474882624680383 1102 18-19 0.32889826919976173 1102 20-21 0.415215210986581 1102 22-23 0.3288210086751775 1102 24-25 0.2937328259451917 1102 26-27 0.3483051117826079 1102 28-29 0.3434623535668919 1102 30-31 0.3221585390169821 1102 32-33 0.32664389282803796 1102 34-35 0.39038437027746653 1102 36-37 0.43412683311327527 1102 38-39 0.49660588155946783 1102 40-41 0.52166999856491 1102 42-43 0.598180250755064 1102 44-45 0.8216770880323807 1102 46-47 0.524312987392257 1102 48-49 0.6746703440998587 1102 50-51 0.5270000741103189 1102 52-53 0.8320150587029644 1102 54-55 0.8380092719050509 1102 56-57 0.6936307062477312 1102 58-59 0.7038736036314965 1102 60-61 0.5953205090186238 1102 62-63 0.4945041727138566 1102 64-65 0.44990045175880056 1102 66-67 0.5827617484281866 1102 68-69 0.6159997620091993 1102 70-71 0.2825745396340622 1102 72-73 0.10157947937567613 1102 74-75 0.4762051060885 1102 76-77 0.5253582600387219 1102 78-79 0.5117474332592273 1102 80-81 0.6536246050399619 1102 82-83 0.6840174054626935 1102 84-85 0.3704312054638841 1102 86-87 0.6875238412513589 1102 88-89 0.42988668051667744 1102 90-91 0.4710532816876203 1102 92-93 0.49982120732301283 1102 94-95 0.6671921601275201 1102 96-97 0.3827010235499415 1102 98-99 0.29688679753612135 1102 100-101 0.10769302662039237 1103 1 0.2833052649229799 1103 2 0.3758805695816676 1103 3 0.2605405638529241 1103 4 0.17032474568191702 1103 5 0.2921253701220863 1103 6 0.329273939824013 1103 7 0.2774119229853156 1103 8 0.34193167413654635 1103 9 0.05548628117746546 1103 10-11 0.4452628305378141 1103 12-13 0.310562915272925 1103 14-15 0.3396097004909109 1103 16-17 0.42307687792144577 1103 18-19 0.410198089397781 1103 20-21 0.3542175600592259 1103 22-23 0.4270640920419311 1103 24-25 0.44982178291628827 1103 26-27 0.33708111943024477 1103 28-29 0.48119024765117047 1103 30-31 0.5433252934929129 1103 32-33 0.5072668019807764 1103 34-35 0.40778689795835277 1103 36-37 0.5941259103900549 1103 38-39 0.43541481364930235 1103 40-41 0.49172682347430197 1103 42-43 0.7131667276977893 1103 44-45 0.7285204357625616 1103 46-47 0.5942810566519938 1103 48-49 0.7293671827404111 1103 50-51 0.5850287178910776 1103 52-53 0.56148809663366 1103 54-55 0.5648967490345811 1103 56-57 0.6342645977425718 1103 58-59 0.644982729848099 1103 60-61 0.6316925807032483 1103 62-63 0.7604320370516007 1103 64-65 0.6431670887649723 1103 66-67 0.32505467046356173 1103 68-69 0.487886740674611 1103 70-71 0.4909557701532279 1103 72-73 0.8613605874888322 1103 74-75 0.6915407982809398 1103 76-77 0.5223502146020209 1103 78-79 0.32246560551874737 1103 80-81 0.706949134862473 1103 82-83 0.7426992146841833 1103 84-85 0.7645917870505237 1103 86-87 0.9166487608760363 1103 88-89 0.755121773293915 1103 90-91 0.8879457628161553 1103 92-93 0.9814478844773227 1103 94-95 1.0536155784557089 1103 96-97 1.1942078660588322 1103 98-99 1.164959477153392 1103 100-101 0.8089020198809642 1104 1 0.2353012309144198 1104 2 0.35751881786019624 1104 3 0.25535278876894196 1104 4 0.25153488146236924 1104 5 0.15917072600312565 1104 6 0.13533096360729502 1104 7 0.19299328105700653 1104 8 0.26969353405493024 1104 9 0.27295607620441587 1104 10-11 0.2968258418851306 1104 12-13 0.13129698461244743 1104 14-15 0.24778975485652666 1104 16-17 0.06880777482046341 1104 18-19 0.21664565730470287 1104 20-21 0.2644243029980373 1104 22-23 0.18655920648355107 1104 24-25 0.28371396669076177 1104 26-27 0.292778488642206 1104 28-29 0.38655367575699273 1104 30-31 0.41876275634196247 1104 32-33 0.6558642493349538 1104 34-35 0.502291229174503 1104 36-37 0.5659254188287477 1104 38-39 0.620306316183914 1104 40-41 0.47954381706932026 1104 42-43 0.359734814949924 1104 44-45 0.7218302596420187 1104 46-47 0.47034616181004907 1104 48-49 0.4356482810059248 1104 50-51 0.6796646219030436 1104 52-53 0.8948297784946462 1104 54-55 0.6673654086617731 1104 56-57 0.8188044602924123 1104 58-59 0.9474158247474769 1104 60-61 0.8114295352939358 1104 62-63 0.7655413359756444 1104 64-65 0.8255852564969111 1104 66-67 0.9396881367054526 1104 68-69 0.9138414782970834 1104 70-71 0.9977618238376031 1104 72-73 0.9706533336201275 1104 74-75 1.1579483266492332 1104 76-77 0.9583878030129327 1104 78-79 1.116287695055032 1104 80-81 1.0322708871182442 1104 82-83 0.8518692374589669 1104 84-85 0.848953992931893 1104 86-87 0.8424538928829541 1104 88-89 0.5793874368015004 1104 90-91 0.6122802181916072 1104 92-93 0.5925629472248559 1104 94-95 0.6959968790895239 1104 96-97 1.0828468556866184 1104 98-99 1.008752012901784 1104 100-101 0.8608655935851068 1105 1 0.21044590915534656 1105 2 0.3212692512282018 1105 3 0.28654861776859164 1105 4 0.22756363042895345 1105 5 0.18930380331597263 1105 6 0.2512002331488645 1105 7 0.20015385390283313 1105 8 0.20311487442882736 1105 9 0.3548266258483821 1105 10-11 0.2374584258251815 1105 12-13 0.22124284694810825 1105 14-15 0.3510793513575763 1105 16-17 0.4570161648249993 1105 18-19 0.2459886714124977 1105 20-21 0.3173778726169445 1105 22-23 0.14812479839757486 1105 24-25 0.17574732935977266 1105 26-27 0.4404283006271683 1105 28-29 0.40772270261246746 1105 30-31 0.3047669700123947 1105 32-33 0.4221275503990398 1105 34-35 0.33690866523605223 1105 36-37 0.3330834581385673 1105 38-39 0.3262327903018445 1105 40-41 0.44962475687783154 1105 42-43 0.6521728766615311 1105 44-45 0.41645782984764423 1105 46-47 0.3582345262125344 1105 48-49 0.2864036747707601 1105 50-51 0.44545121816408084 1105 52-53 0.46947575017238563 1105 54-55 0.4822519329170447 1105 56-57 0.7653641850703963 1105 58-59 0.8243453124398279 1105 60-61 0.7445948551861967 1105 62-63 0.7374490885958522 1105 64-65 0.4921939185232027 1105 66-67 0.5668389754392216 1105 68-69 0.8302967947223969 1105 70-71 0.6281375872320076 1105 72-73 0.6756544003721316 1105 74-75 1.0086017122534159 1105 76-77 0.6295554964284023 1105 78-79 0.7314975785123785 1105 80-81 0.8218953904118429 1105 82-83 0.7122427340053576 1105 84-85 1.0795377729676296 1105 86-87 0.8913771400760631 1105 88-89 0.9423444733644253 1105 90-91 0.9207802315260061 1105 92-93 0.7873771990316669 1105 94-95 0.9804909719502888 1105 96-97 1.191328867580907 1105 98-99 1.4538102842301548 1105 100-101 1.023862633348287 1106 1 0.1041475634599216 1106 2 0.18003967518197683 1106 3 0.21411791411487613 1106 4 0.06829043163104842 1106 5 0.05988583417838811 1106 6 0.06924405978328707 1106 7 0.10180183811415588 1106 8 0.01611294297964605 1106 9 0.22344594550526153 1106 10-11 0.04369421349897351 1106 12-13 0.029334477289665983 1106 14-15 0.2284338572341511 1106 16-17 0.2792263353117548 1106 18-19 0.21265044489333462 1106 20-21 0.07178496800824519 1106 22-23 0.20464245396350123 1106 24-25 0.08955250172903817 1106 26-27 -0.027571793158898572 1106 28-29 0.0960531000343039 1106 30-31 0.10236526528081669 1106 32-33 0.2431427981097869 1106 34-35 0.11960432131033372 1106 36-37 0.26216352561015555 1106 38-39 0.4463926139840453 1106 40-41 0.194260601910635 1106 42-43 0.35125452082633757 1106 44-45 0.3572927568644104 1106 46-47 0.45148063850842846 1106 48-49 0.3912237006774646 1106 50-51 0.5156755707344303 1106 52-53 0.525715785403321 1106 54-55 0.4219979561841427 1106 56-57 0.4994823990031989 1106 58-59 0.5731031568283633 1106 60-61 0.6306167562704452 1106 62-63 0.6149949044462382 1106 64-65 0.5725536220505134 1106 66-67 0.20042940491142147 1106 68-69 0.23609821021232236 1106 70-71 0.005792650252164577 1106 72-73 0.17969773496953678 1106 74-75 0.2359496599017632 1106 76-77 0.1044617783275612 1106 78-79 0.17476091890005563 1106 80-81 0.10485133678796288 1106 82-83 0.20669015740321228 1106 84-85 0.44013634551658853 1106 86-87 0.2333751501804926 1106 88-89 0.1972605960039715 1106 90-91 0.44076889564550115 1106 92-93 0.33832936542173186 1106 94-95 0.2316932005245924 1106 96-97 0.42199296804344755 1106 98-99 0.5208073428817848 1106 100-101 0.5472755081357406 1107 1 0.15314837939131465 1107 2 0.23584570613018485 1107 3 0.05484620011091579 1107 4 0.07619342668133555 1107 5 -0.06115760489243627 1107 6 -0.02923855291276567 1107 7 0.018260266971374506 1107 8 -0.010770243353285025 1107 9 -0.0363208077243371 1107 10-11 0.11086422507587201 1107 12-13 0.18098708726594737 1107 14-15 0.26058587713239945 1107 16-17 0.1566620028488117 1107 18-19 0.16731604407529233 1107 20-21 0.20081837353976084 1107 22-23 0.0958613029658153 1107 24-25 0.3036858154555091 1107 26-27 0.14881848142908183 1107 28-29 0.036390712497187394 1107 30-31 0.24727648466083707 1107 32-33 0.20104948646654464 1107 34-35 0.23666545693568963 1107 36-37 0.33817007076193306 1107 38-39 0.3749848310810293 1107 40-41 0.4353642435788956 1107 42-43 0.5243230321719423 1107 44-45 0.500455357319801 1107 46-47 0.4737116202777898 1107 48-49 0.6424990827985155 1107 50-51 0.28462197762380015 1107 52-53 0.385718195413439 1107 54-55 0.527128009929406 1107 56-57 0.3228177645060839 1107 58-59 0.3149420558213727 1107 60-61 0.39956009958762095 1107 62-63 0.0678114676152255 1107 64-65 0.13619439846863202 1107 66-67 0.26811103304345707 1107 68-69 0.3077589080191778 1107 70-71 0.1882247997871609 1107 72-73 0.12225541607686452 1107 74-75 0.1125169749224284 1107 76-77 -0.046795630836676594 1107 78-79 0.0037555532235664657 1107 80-81 0.1526202563034964 1107 82-83 0.14130467662491597 1107 84-85 0.11642211825331472 1107 86-87 0.3762652629097367 1107 88-89 0.46372142465940414 1107 90-91 0.2659236826302198 1107 92-93 0.292604188951465 1107 94-95 0.5626219700884754 1107 96-97 0.8598092605968901 1107 98-99 0.49790543880359195 1107 100-101 0.4913540826351799 1108 1 0.01866166677871206 1108 2 0.09132679295366586 1108 3 -0.11635147397272405 1108 4 0.018460759514880465 1108 5 0.033099481093536554 1108 6 -0.018340386932663932 1108 7 0.06760237916817147 1108 8 -0.010053231014772734 1108 9 0.06068488411001027 1108 10-11 -0.025198439202860357 1108 12-13 -0.11674815847501208 1108 14-15 -0.11481118469788498 1108 16-17 0.08918748822858191 1108 18-19 0.024970651397744348 1108 20-21 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-1.4442210995390248 2316 80-81 -1.2084508989627771 2316 82-83 -1.283160077854557 2316 84-85 -1.172961274218391 2316 86-87 -1.5777711891005488 2316 88-89 -1.6753667783099644 2316 90-91 -1.9746877171490773 2316 92-93 -1.422988246918706 2316 94-95 -1.5709637688285873 2316 96-97 -1.2940702333193101 2316 98-99 -1.1543098639407745 2316 100-101 -1.3232229817237808 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 17.0 4 43.0 5 108.0 6 216.0 7 447.0 8 966.0 9 1818.0 10 3056.0 11 4606.0 12 6576.0 13 9276.0 14 11518.0 15 15212.0 16 19410.0 17 24616.0 18 21390.0 19 15044.0 20 14226.0 21 14820.0 22 16029.0 23 17815.0 24 20579.0 25 23769.0 26 28747.0 27 34498.0 28 41914.0 29 49865.0 30 58914.0 31 71099.0 32 86235.0 33 105292.0 34 133238.0 35 168745.0 36 203957.0 37 194550.0 38 67179.0 39 1452.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.34303121185936 19.76349863324132 15.301846889405182 20.591623265494135 2 16.088082431190223 22.518890705241787 37.910116756897786 23.4829101066702 3 20.971042217911258 26.370443091385287 29.99216630299933 22.666348387704122 4 13.060407747758774 17.458814733032867 37.21799329363124 32.26278422557712 5 12.83570608218058 39.29492645061338 32.982710716504705 14.886656750701336 6 30.109948186110437 37.205479120468304 16.86862832376083 15.815944369660434 7 27.576730904095697 34.58136968874622 20.2155935512892 17.62630585586888 8 27.282654375153392 32.45578114651309 19.2001102739703 21.061454204363216 9 26.977783097080106 17.019511230825294 20.638952802697066 35.36375286939753 10-11 25.42245869210608 26.739523925429953 26.034193401764067 21.8038239806999 12-13 27.789081295226207 24.540425726476993 26.718310219022275 20.952182759274525 14-15 23.659925882868304 25.491539532442804 27.431104787692373 23.417429796996515 16-17 21.236337082346154 28.870861620712596 27.66741875541919 22.225382541522055 18-19 21.03073623680717 27.24529045328578 30.954328499813577 20.769644810093475 20-21 22.817780434448608 25.064053148059678 30.730819439882495 21.387346977609216 22-23 21.970189797561595 25.37646012671101 31.045374241255207 21.60797583447219 24-25 22.48821811663342 25.56902344677157 31.047052898622347 20.89570553797266 26-27 21.888458189471134 25.190200673056555 31.960033484731838 20.96130765274047 28-29 20.99408638119476 26.827220800876788 31.265460633587605 20.913232184340842 30-31 21.465927398207143 25.516495028361337 31.118564908326583 21.899012665104934 32-33 21.49649155778465 25.850617789084644 31.082434523703462 21.570456129427242 34-35 22.515581772746664 25.451433516368805 30.900843142086877 21.132141568797657 36-37 22.31091797073959 25.10073246834807 31.38687554694747 21.201474013964873 38-39 21.819336540310683 25.833796179868664 31.263490351498884 21.08337692832177 40-41 21.762614174996664 26.089102303553783 30.752084978572803 21.39619854287675 42-43 21.454948524798937 26.342050038292896 29.843210928301843 22.35979050860632 44-45 22.067558071901907 25.710193556195847 29.779479748380304 22.442768623521935 46-47 22.936992266242438 26.613115785821577 29.12067001693062 21.329221931005364 48-49 22.672581051309436 26.64902302716845 28.749080079867056 21.929315841655058 50-51 21.808851483058113 27.12409840838444 29.263039837120125 21.80401027143732 52-53 22.657663851493894 27.51804771057231 27.88231342854559 21.941975009388205 54-55 21.806086833153696 26.84497422411805 28.467977324485645 22.880961618242615 56-57 22.772993626461417 26.8110586744815 27.96401822086954 22.451929478187544 58-59 23.63359337072744 26.327777561117305 27.773997426073578 22.26463164208168 60-61 22.435414900909727 26.84509763115967 29.218443058772348 21.501044409158258 62-63 22.16530856087405 26.913221309496834 28.95986062802111 21.96160950160801 64-65 21.856656038235457 27.70840085368133 28.411766762640816 22.023176345442394 66-67 22.327003110815706 27.041100891892683 27.77424212319476 22.857653874096854 68-69 22.174347760893962 26.99144647440229 28.176507490806692 22.657698273897058 70-71 22.850729011766866 27.03212009000901 27.85962333523514 22.25752756298899 72-73 22.587502538303163 26.5686377347174 28.568686147386995 22.275173579592447 74-75 21.899418650978436 28.39522319451808 27.50862475031848 22.196733404185 76-77 22.217130723355556 27.141357229235453 27.708082517246 22.933429530162986 78-79 22.32692983995637 27.28955315410545 27.885791479984967 22.49772552595321 80-81 22.733076746184476 27.412362982059076 27.53201623976106 22.322544031995395 82-83 22.476936525013446 27.15135825712749 28.0749058633674 22.29679935449166 84-85 21.995650943136148 27.67134497340338 27.797486449541054 22.535517633919415 86-87 22.159660336926457 27.844189079290242 27.195671709682074 22.80047887410123 88-89 21.86804696845303 27.856571797814837 26.796096855120087 23.479284378612046 90-91 22.259635735460197 27.189892501244845 27.08986785651725 23.460603906777703 92-93 22.650220194411208 27.65513376234738 27.28739783291986 22.40724821032155 94-95 22.162899061348536 27.362396116295955 27.348410478470186 23.126294343885316 96-97 21.414348087586195 27.383612703635958 27.96875562071712 23.233283588060733 98-99 21.868392732690957 27.965678603018823 26.662186318172203 23.503742346118013 100-101 21.602738256892795 27.551070837705293 26.918751620049036 23.927439285352875 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1408.0 1 1023.5 2 1063.0 3 1378.5 4 1041.5 5 855.0 6 1297.0 7 2454.0 8 3593.5 9 4013.0 10 3516.5 11 2806.0 12 2648.0 13 2760.5 14 2865.5 15 3006.5 16 3146.0 17 3324.5 18 3604.5 19 3846.0 20 4153.5 21 4553.5 22 5023.5 23 5682.0 24 6482.0 25 7380.5 26 8723.0 27 10419.5 28 12116.5 29 14232.0 30 17063.5 31 19977.5 32 23396.5 33 26003.0 34 27626.5 35 29404.0 36 32315.5 37 35416.0 38 36567.0 39 37056.0 40 37698.0 41 39395.5 42 41042.0 43 43002.0 44 45119.0 45 47365.5 46 49782.0 47 59376.5 48 80507.5 49 79185.0 50 58963.0 51 49425.5 52 45795.0 53 45389.5 54 45238.5 55 42438.0 56 38421.0 57 35089.0 58 33249.0 59 30466.5 60 25643.5 61 21938.5 62 18980.0 63 14671.0 64 10616.0 65 8221.0 66 6161.5 67 4728.5 68 3672.0 69 2665.0 70 2093.5 71 1729.5 72 1448.5 73 1327.0 74 1093.5 75 868.0 76 661.0 77 423.5 78 269.5 79 176.5 80 126.5 81 91.0 82 58.0 83 36.5 84 23.5 85 14.0 86 9.5 87 4.5 88 1.5 89 2.0 90 2.5 91 1.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010152994191949415 2 0.009346133726364031 3 0.005446308142701342 4 2.01715116396346E-4 5 0.005513546514833457 6 0.0 7 2.01715116396346E-4 8 0.0027567732574167287 9 0.0 10-11 0.0 12-13 0.0 14-15 3.6981104672663434E-4 16-17 0.0036644912812002855 18-19 0.0018490552336331715 20-21 3.02572674594519E-4 22-23 2.3533430246240364E-4 24-25 3.3619186066057667E-4 26-27 6.723837213211533E-5 28-29 6.723837213211533E-5 30-31 6.723837213211533E-4 32-33 0.0031938226762754786 34-35 7.732412795193263E-4 36-37 9.749563959156724E-4 38-39 0.004841162793512304 40-41 3.361918606605767E-5 42-43 0.0016809593033028833 44-45 0.005446308142701342 46-47 0.0 48-49 0.0016473401172368257 50-51 0.00100857558198173 52-53 5.04287790990865E-4 54-55 2.01715116396346E-4 56-57 0.010758139541138453 58-59 0.001613720931170768 60-61 0.0027903924434827866 62-63 2.01715116396346E-4 64-65 0.0026895348852846133 66-67 0.0010421947680477877 68-69 0.0015464825590386527 70-71 0.004135159886125093 72-73 0.0021516279082276907 74-75 0.006824694771409705 76-77 0.0011766715123120183 78-79 0.005984215119758264 80-81 0.0021516279082276907 82-83 0.0030929651180773053 84-85 0.001815436047567114 86-87 5.04287790990865E-4 88-89 0.0023533430246240367 90-91 0.007564316864862975 92-93 0.002521438954954325 94-95 4.03430232792692E-4 96-97 1.6809593033028833E-4 98-99 7.06002907387211E-4 100-101 0.001512863372972595 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1487246.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.357460218584116 #Duplication Level Percentage of deduplicated Percentage of total 1 76.26650470550136 38.40587476717743 2 13.178576556175264 13.272792893303222 3 4.3910901445850214 6.633724418164709 4 1.9730283974355662 3.974267961359932 5 1.0955482791733493 2.7584514443005115 6 0.6500132239485049 1.9639809039924265 7 0.4350401507868946 1.5335261960716515 8 0.3053032114725956 1.2299435461069765 9 0.23190154736390609 1.05101756514054 >10 1.303945676043412 12.228155578156361 >50 0.10828847999561411 3.7232332332663662 >100 0.05299745178087162 4.992823730104862 >500 0.0036134266365433015 1.224813091027727 >1k 0.0034795960203750313 3.795680538118885 >5k 4.0149184850481136E-4 1.4898051166644068 >10k+ 2.6766123233654083E-4 1.721909017044042 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 14375 0.9665515993991579 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11175 0.7513888085763888 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9289 0.6245772387352193 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6458 0.43422540722920083 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6359 0.4275688083881214 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4738 0.3185754071619625 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4265 0.28677165714347186 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3753 0.25234561061182886 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3621 0.24347014549038962 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3426 0.23035866292462712 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3096 0.20817000012102907 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2727 0.1833590408042785 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2633 0.17703863382385968 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2411 0.16211171521053006 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2332 0.15679988381209295 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2231 0.1500088082267493 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2198 0.1477899419463895 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1858 0.12492889542147027 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1776 0.11941534890663683 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1703 0.11450694774099242 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1524 0.10247127912934376 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.361918606605767E-5 0.0 12-13 0.0 0.0 0.0 6.723837213211533E-5 0.0 14-15 0.0 0.0 0.0 6.723837213211533E-5 0.0 16-17 0.0 0.0 0.0 6.723837213211533E-5 0.0 18-19 0.0 0.0 0.0 6.723837213211533E-5 0.0 20-21 0.0 0.0 0.0 2.01715116396346E-4 0.0 22-23 0.0 0.0 0.0 3.3619186066057667E-4 0.0 24-25 0.0 0.0 0.0 4.03430232792692E-4 0.0 26-27 0.0 0.0 0.0 8.404796516514417E-4 0.0 28-29 0.0 0.0 0.0 0.0020843895360955753 0.0 30-31 0.0 0.0 0.0 0.004942020351710477 0.0 32-33 0.0 0.0 0.0 0.009581468028826435 0.0 34-35 0.0 0.0 0.0 0.015229491287924123 0.0 36-37 0.0 0.0 0.0 0.020272369197832773 0.0 38-39 0.0 0.0 0.0 0.02797116280695998 0.0 40-41 0.0 0.0 0.0 0.04004045060467468 0.0 42-43 0.0 0.0 0.0 0.05409327038028679 0.0 44-45 0.0 0.0 0.0 0.07157524713463678 0.0 46-47 0.0 0.0 0.0 0.0949069622644808 0.0 48-49 0.0 0.0 0.0 0.1210290698378076 0.0 50-51 0.0 0.0 0.0 0.1512863372972595 0.0 52-53 0.0 0.0 0.0 0.1936128925544261 0.0 54-55 0.0 0.0 0.0 0.2371497385099708 0.0 56-57 0.0 0.0 0.0 0.2923188228443714 0.0 58-59 0.0 0.0 0.0 0.3600614827674776 0.0 60-61 0.0 0.0 0.0 0.43983981130223243 0.0 62-63 0.0 0.0 0.0 0.5188448985574681 0.0 64-65 0.0 0.0 0.0 0.60043866313979 0.0 66-67 0.0 0.0 0.0 0.7057003347126165 0.0 68-69 0.0 0.0 0.0 0.8328144772283805 0.0 70-71 0.0 0.0 0.0 0.9757296371951916 0.0 72-73 0.0 0.0 0.0 1.1066427477364202 0.0 74-75 0.0 0.0 0.0 1.2585006111968027 0.0 76-77 0.0 0.0 0.0 1.4128799136121395 0.0 78-79 0.0 0.0 0.0 1.5857833875498741 0.0 80-81 0.0 0.0 0.0 1.7875993615044181 0.0 82-83 0.0 0.0 0.0 2.0128815273330707 0.0 84-85 0.0 0.0 0.0 2.241189419907668 0.0 86-87 0.0 0.0 0.0 2.4913497834252034 0.0 88-89 0.0 0.0 0.0 2.7817186934777434 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGGTG 690 0.0 25.471762 5 GTACATG 10720 0.0 23.579002 1 GTAAGGT 620 0.0 22.98532 4 TACATGG 10885 0.0 22.696259 2 GAGTACT 10260 0.0 22.545443 12-13 GGTAAGG 640 0.0 22.268524 3 AGGTAAG 600 0.0 22.171045 2 AGTACTT 10485 0.0 22.152237 12-13 ACATGGG 10875 0.0 21.405088 3 GTACTTT 10800 0.0 21.33021 14-15 TACCTGG 1095 0.0 20.392155 2 GTAAGCG 210 0.0 20.355686 94-95 TACTTTT 11555 0.0 19.85429 14-15 CCTATAC 315 3.2014214E-10 19.605728 3 CATGGGA 6895 0.0 19.22166 4 ACTTTTT 12105 0.0 19.089209 16-17 AGAGTAC 18030 0.0 18.967669 10-11 GTACCTG 1280 0.0 18.559599 1 ATGGGAG 2475 0.0 18.040934 5 GTGTAGC 765 0.0 18.011305 1 >>END_MODULE